CN1117080A - Bacillus thuringiensis culture medium - Google Patents
Bacillus thuringiensis culture medium Download PDFInfo
- Publication number
- CN1117080A CN1117080A CN 95100402 CN95100402A CN1117080A CN 1117080 A CN1117080 A CN 1117080A CN 95100402 CN95100402 CN 95100402 CN 95100402 A CN95100402 A CN 95100402A CN 1117080 A CN1117080 A CN 1117080A
- Authority
- CN
- China
- Prior art keywords
- powder
- culture medium
- bacillus thuringiensis
- substratum
- tea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The said culture medium is used for fermentation prodn of bacillus thuringiensis. Its main effective components are carbon source material, nitrogen source material, and mineral elements. It contains 0.5-2.5% of tea cake powder. Compared with the available techn, the said culture medium has the advantage that the fermentation liquid for producing bacillus thuringiensis has the associated matter to cause insects refusing eating, so that the cultured bacillus thuringiensis has higher insect-killing rate. The said culture medium shortens the fermentation period by 8-12 hr.
Description
The present invention relates to a kind of bacteria culture medium, particularly bacillus thuringiensis culture medium, be used for the fermentative production Tribactur, its effective constituent is mainly carbon source material, nitrogen source and mineral element.
Tribactur acts on the insect, be that a kind of people is held has no side effect, harmless boilogical agricultural chemicals free from environmental pollution, but, it also exist drug effect slowly, before insect death, do not influence weak point such as its appetite, metainfective insect when its dead peak generally costs three days during this period, is got the insect that food restricted and can continue to produce harm to plant.
In the prior art, once used the agricultural chemicals and the Tribactur that can produce the insect antifeedant material to use with, used inconvenience but exist, weak points such as use cost raising, and also some agricultural chemicals of using with can produce Tribactur and subtract the effect effect.
In the prior art, the nitrogen source of the substratum of Su Yun bacillus adopts soybean cake powder, cottonseed meal, fish meal etc. more, and these materials also have been simple effect as nitrogen source.Weak points such as in addition, carbon source material of the prior art and mineral element are combined and used in the substratum, and in fermentative production Tribactur process, it is low to exist fermentation rate, and fermentation period is long.
In the prior art, people mix withered powder of tea and Tribactur before sprinkling, to play dispersion agent and adhesive agent effect, in addition, and even do not see the application of the withered powder of tea in the Tribactur bacteria preparation is produced.
The objective of the invention is to overcome prior art can weak point, and provide a kind of raising fermentation rate, shorten fermentation period, fermented liquid can produce the bacillus thuringiensis culture medium of insect antifeedant material.
The objective of the invention is to realize by following approach.
The bacillus thuringiensis culture medium that is used for fermentative production, its effective constituent is mainly carbon source material, nitrogen source and mineral element have the withered powder of tea in the nitrogen source of being made up of at least a raw material, and the content of the withered powder of said tea in substratum is 0.5～2.5%.
Here, the withered powder of tea is not only as nitrogen source, and can produce a kind of insect antifeedant material and Tribactur coexists as in the fermented liquid.
Also find in contriver's research, as nitrogen source, the withered powder of tea has specificity in its ferment effect, but, using with substratum combination of active principles such as carbon source material and mineral elements, but in contrast, have compatibleness widely on this problem, do not see that it repels certain as the raw material of carbon source material or mineral element and can not coexist as in the same substratum.Like this, the withered powder of tea can wide spectrum be applied in the bacillus thuringiensis culture medium of all kinds and as nitrogen source.
Purpose of the present invention can also reach by following measure.
The content of the withered powder of said tea in substratum is 1.34%.
The raw material of said nitrogen source and the content in substratum are analysis for soybean powder 2%, fish meal 0.2%, peptone 0.1%, the withered powder 1.34% of tea.
The concrete raw material of composition substratum effective constituent and the content in substratum are analysis for soybean powder 2%, Semen Maydis powder 1%, fish meal 0.2%, peptone 0.1%, yeast powder 0.1%, MgSO
47H
2O0.01%, CaCO
30.05%, MnSO
4H
2O0.001%, the withered powder 1.34% of tea.
Form the concrete raw material of substratum effective constituent and the content in the substratum thereof and be soybean cake powder 0.5%, cottonseed meal 1.5%, Semen Maydis powder 1%, yeast powder 0.2%, MgSO
47H
2O0.005%, CaCO
30.02%, peptone 0.05%, the withered powder 2% of tea, MnSO
4H
2O0.0005%.
In sum, the present invention has following advantage compared to existing technology: owing to the withered powder of tea is got involved use in substratum as nitrogen source, association has the insect antifeedant material in the fermented liquid of fermentative production Tribactur, insect antifeedant in 4～7 hours after making it to use, wait for and infecting, thereby improve insecticidal rate 78%, having remedied Tribactur did not influence the weak point of aspects such as its appetite in the past in desinsection speed and at insect death, improved fermentation rate, shorten fermentation period 8～12 hours, thereby raising output reduces production cost; Easy to use, because remix does not use external insect antifeedant material, so reduced the desinsection cost.
Below we carry out more detailed description to the present invention in conjunction with the embodiments.
Embodiment 1:
Under standard state, the preparation bacillus thuringiensis culture medium.
Analysis for soybean powder 20 grams, Semen Maydis powder 10 grams, fish meal 2 grams, peptone 1 gram, yeast powder 1 gram, MgSO
47H
2The O0.1 gram, CaCO
30.5 gram, MnSO
4H
2The O0.001 gram, withered powder 13.4 grams of tea.Above all raw materials are mixed, add 1 liter in water (1000 gram), stir, a kind of bacillus thuringiensis culture medium of the present invention.
It is same as the prior art that the present invention does not state part.
Most preferred embodiment:
Under standard state, the preparation bacillus thuringiensis culture medium.
Analysis for soybean powder 5 grams, cottonseed meal 15 grams, Semen Maydis powder 10 grams, fish meal 2 grams, peptone 1 gram, yeast powder 2 grams, withered powder 20 grams of tea, MgSO
47H
2The O0.005 gram, CaCO
30.2 gram, MnSO
4H
2The O0.005 gram, peptone 0.5 gram.Above all raw materials are mixed, add 1 liter in water (1000 gram), stir, a kind of bacillus thuringiensis culture medium of the present invention.
It is same as the prior art that the present invention does not state part.
Claims (5)
1, the bacillus thuringiensis culture medium that is used for fermentative production, its effective constituent is mainly carbon source material, and nitrogen source and mineral element is characterized in that, have the withered powder of tea in the nitrogen source of being made up of at least a raw material, the content of the withered powder of said tea in substratum is 0.5～2.5%.
2, bacillus thuringiensis culture medium according to claim 1 is characterized in that, the content of the withered powder of said tea in substratum is 1.34%.
3, bacillus thuringiensis culture medium according to claim 1 is characterized in that, the raw material of said nitrogen source and the content in substratum are analysis for soybean powder 2%, fish meal 0.2%, peptone 0.1%, the withered powder 1.34% of tea.
4, bacillus thuringiensis culture medium according to claim 1 is characterized in that, the concrete raw material of composition substratum effective constituent and the content in substratum are, analysis for soybean powder 2%, Semen Maydis powder 1%, fish meal 0.2%, peptone 0.1%, yeast powder 0.1%, MgSO
47H
2O0.01%, CaCO
30.05%, MnSO
4H
2O0.001%, the withered powder 1.34% of tea.
5, bacillus thuringiensis culture medium according to claim 1 is characterized in that, forms the concrete raw material of substratum effective constituent and the content in the substratum thereof and is soybean cake powder 0.5%, cottonseed meal 1.5%, Semen Maydis powder 1%, yeast powder 0.2%, MgSO
47H
2O0.005%, CaCO
30.02%, peptone 0.05%, the withered powder 2% of tea, MnSO
4H
2O0.0005%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95100402 CN1117080A (en) | 1995-01-11 | 1995-01-11 | Bacillus thuringiensis culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95100402 CN1117080A (en) | 1995-01-11 | 1995-01-11 | Bacillus thuringiensis culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1117080A true CN1117080A (en) | 1996-02-21 |
Family
ID=5073433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95100402 Pending CN1117080A (en) | 1995-01-11 | 1995-01-11 | Bacillus thuringiensis culture medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1117080A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060801C (en) * | 1996-05-21 | 2001-01-17 | 北京市卫生防疫站 | Method of bacteria culture preservation |
| US7470715B2 (en) | 2005-12-22 | 2008-12-30 | Pfizer Inc. | Estrogen modulators |
| CN102796683A (en) * | 2012-08-07 | 2012-11-28 | 福建农林大学 | Glucoside fermentation medium for bacillus thuringiensis |
-
1995
- 1995-01-11 CN CN 95100402 patent/CN1117080A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1060801C (en) * | 1996-05-21 | 2001-01-17 | 北京市卫生防疫站 | Method of bacteria culture preservation |
| US7470715B2 (en) | 2005-12-22 | 2008-12-30 | Pfizer Inc. | Estrogen modulators |
| CN102796683A (en) * | 2012-08-07 | 2012-11-28 | 福建农林大学 | Glucoside fermentation medium for bacillus thuringiensis |
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| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| AD01 | Patent right deemed abandoned | ||
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |