CN111707659A - Luminous bacteria-based water quality comprehensive biotoxicity analyzer - Google Patents
Luminous bacteria-based water quality comprehensive biotoxicity analyzer Download PDFInfo
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- CN111707659A CN111707659A CN202010646641.XA CN202010646641A CN111707659A CN 111707659 A CN111707659 A CN 111707659A CN 202010646641 A CN202010646641 A CN 202010646641A CN 111707659 A CN111707659 A CN 111707659A
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Abstract
The invention discloses a luminous bacteria-based water quality comprehensive biotoxicity analyzer, which comprises a reagent storage unit, a sample channel, a reference channel, a photoelectric detection unit and a driving mechanism, wherein the reagent storage unit is arranged in the sample channel; wherein, the reagent storage unit stores salt solution and bacteria solution cultured with luminous bacteria; the sample channel and the reference channel are respectively provided with a multi-channel selection valve and an injector, under the control of the driving mechanism, the sample/reference water sample to be detected and various reagents can be subjected to parallel, automatic and equivalent sample introduction, mixing and reaction, and a mixed liquid is formed and automatically pushed to the photoelectric detection unit for light intensity detection, so that the judgment of water quality comprehensive biotoxicity is realized. The analyzer combines a luminous bacteria detection method with a flow injection technology, adopts a modern photoelectric detection means, and can quickly and effectively monitor the comprehensive biotoxicity of the environmental water body.
Description
Technical Field
The invention belongs to the technical field of water quality monitoring, and particularly relates to a measuring instrument for analyzing biotoxicity in a water body by utilizing luminous bacteria.
Background
With the continuous expansion of the activity range of human production and life, the types and the pollution degree of pollutants in the environmental water body are also greatly changed. The traditional water quality monitoring technology mainly analyzes conventional chemical, physical or biological parameters (such as COD, dissolved oxygen, conductivity, total number of bacteria and the like), although the indexes can reflect the pollution degree of water quality, the traditional water quality monitoring technology cannot carry out comprehensive toxicity effect evaluation on environmental water and reveal the comprehensive harm degree of pollutants to human beings, so that the online monitoring of the comprehensive toxicity of the water quality is enhanced, and important basis can be provided for early warning and evaluation decisions of the water body. Particularly, since new coronary pneumonia occurs in 2020, the strengthening of the early warning and monitoring of water quality in drinking water sources is clearly pointed out in the emergency monitoring scheme for the epidemic situation of pneumonia caused by the infection of novel coronavirus issued by the department of ecological environment, and in the epidemic situation prevention and control period, the monitoring of the epidemic situation prevention and control characteristic indexes such as residual chlorine, biotoxicity and the like is increased on the basis of the conventional monitoring of the drinking water sources. Therefore, the development of a water quality comprehensive biotoxicity analyzer which is efficient, rapid and simple to operate is urgent.
The existing water quality comprehensive toxicity analysis technology mainly adopts luminous bacteria as a detection living body, and the toxicity of pollutants is represented by utilizing the characteristic that the luminous intensity of the luminous bacteria is changed after the luminous bacteria is contacted with toxic substances, so that the chemical toxic substances are quickly and accurately monitored. Luminescent bacteria are a class of bacteria that are capable of emitting blue-green fluorescence under normal physiological conditions at wavelengths between 450nm and 490nm that are visible to the naked eye. The use of luminescent bacteria to produce biosensors is one of the hot spots in research.
However, different from common chemical reagents, luminescent bacteria have high requirements on culture, revival, storage and the like. Especially, when the water quality is used for on-line monitoring and analysis, the function of the biological analysis method can be exerted to the maximum extent only by effectively combining with hardware technologies such as photoelectric detection, flow analysis, signal acquisition and analysis and the like.
Disclosure of Invention
The invention aims to provide a water quality comprehensive biotoxicity analyzer based on luminous bacteria, which can quickly and effectively monitor the comprehensive biotoxicity of an environmental water body by combining a luminous bacteria detection method with a flow injection technology and adopting a modern photoelectric detection means.
In order to solve the technical problems, the invention adopts the following technical scheme:
a water quality comprehensive biotoxicity analyzer based on luminous bacteria comprises a reagent storage unit, a sample channel, a reference channel, a photoelectric detection unit and a driving mechanism; wherein, a salt solution and a bacterium solution cultured with luminous bacteria are stored in the reagent storage unit; the sample channel comprises a first multi-channel selection valve and a first syringe; a first selection channel of the first multi-channel selection valve is communicated with the bacterial liquid, a second selection channel is communicated with the salt liquid, a fifth selection channel is used for being communicated with a water sample to be detected, and a common channel is communicated with the first injector; the reference channel comprises a second multi-channel selection valve and a second injector; a first selection channel of the second multi-channel selection valve is communicated with the bacterial liquid, a second selection channel is communicated with the salt liquid, a fifth selection channel is used for being communicated with sterile pure water, and a common channel is communicated with the second injector; the photoelectric detection unit comprises a sample detection cell and a reference detection cell; the sample detection cell is communicated with a third selection channel of the first multi-channel selection valve, and the reference detection cell is communicated with a third selection channel of the second multi-channel selection valve; a first photomultiplier is arranged on the sample detection pool and is used for detecting the luminous intensity of the mixed liquid in the sample detection pool; a second photomultiplier is arranged on the reference detection pool and is used for detecting the luminous intensity of the mixed liquid in the reference detection pool; the driving mechanism is connected with the pull rods of the first injector and the second injector; in the water quality analysis process, the driving mechanism drives the pull rod to synchronously control the first injector and the second injector to correspondingly extract equal amounts of bacteria liquid, equal amounts of salt liquid, equal amounts of water samples to be detected and sterile pure water through the first multi-channel selection valve and the second multi-channel selection valve one by one, and after the first injector and the second injector are mixed, the pull rod is pushed to correspondingly push mixed liquid to the sample detection pool and the reference detection pool through third selection channels of the first multi-channel selection valve and the second multi-channel selection valve respectively.
In some embodiments of the present application, the analyzer further comprises a signal acquisition module and a control module; the signal acquisition module is connected with the first photomultiplier and the second photomultiplier and is used for converting detection signals which are output by the first photomultiplier and the second photomultiplier and reflect the luminous intensity of the mixed liquid into light intensity data; the control module is used for controlling the driving mechanism, the first multi-channel selection valve and the second multi-channel selection valve and receiving the light intensity data output by the signal acquisition module so as to be used for analyzing the comprehensive biological toxicity of the water sample to be detected. The signal acquisition module and the control module are integrated in the analyzer, so that the analyzer has the capabilities of automatically acquiring detection data and automatically generating a detection result, the automation degree of the analyzer is improved, and the online monitoring of the water quality comprehensive biotoxicity can be realized.
In some embodiments of the present application, the control module preferably performs the following water quality detection process in the water quality analysis process:
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the first selection channel of the first multi-channel selection valve, and controlling the driving mechanism to drive the first injector and the second injector to respectively extract 0.5-2.5mL of bacterial liquid;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with a fifth selection channel of the first multi-channel selection valve and controlling the driving mechanism to drive the first injector and the second injector to correspondingly extract 2-8mL of water sample to be detected and sterile pure water;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the second selection channel of the first multi-channel selection valve and controlling the driving mechanism to drive the first injector and the second injector to respectively extract quantitative salt liquid, wherein the extraction volume of the salt liquid is 1/10 of the extraction volume of the water sample to be detected;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with a third selection channel of the first multi-channel selection valve and controlling the driving mechanism to push and pull the pull rods of the first injector and the second injector for multiple times, so that the liquid in the injectors is correspondingly pushed to the sample detection pool and the reference detection pool after being fully mixed;
after the mixed liquid is pushed to the sample detection pool and the reference detection pool, the first photomultiplier and the second photomultiplier are started and timing is started, the signal acquisition module acquires the luminous intensity of the mixed liquid detected when the timing reaches t by the first photomultiplier and the second photomultiplier, and the formula H (%) =100 × (1-I)st/Ict) Calculating the relative luminescence inhibition rate after the reaction time t, and judging the total volume of the water sample to be detected according to the relative luminescence inhibition rateSynbiotics; wherein, IstThe luminous intensity of the mixed liquid in the sample detection pool is obtained; i isctThe luminous intensity of the mixed liquid in the detection pool is taken as reference.
In some embodiments of the present application, to ensure parallelism of the measurements, the control module periodically initiates a negative detection process: replacing the water sample to be detected with sterile pure water, repeatedly executing the water quality detection process, and calculating the relative luminescence inhibition rate H (%); if the relative luminescence inhibition rate H (%) is within +/-10%, judging that the luminescent bacteria in the bacterial liquid are normal and the parallelism of an analyzer meets the requirement; otherwise, replacing the bacteria liquid or detecting whether the analyzer has a fault.
In some embodiments of the present application, to achieve a positive detection, a positive quality control sample is also stored in the reagent storage unit; the first multi-channel selection valve and the second multi-channel selection valve are respectively communicated with the positive property control sample through a fourth selection channel of the first multi-channel selection valve and the second multi-channel selection valve; the control module periodically initiates the following positive detection process:
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the first selection channel of the first multi-channel selection valve, and controlling the driving mechanism to drive the first injector and the second injector to respectively extract 0.5-2.5mL of bacterial liquid;
controlling a common channel of the first multi-channel selection valve to be communicated with a fourth selection channel of the first multi-channel selection valve, controlling a common channel of the second multi-channel selection valve to be communicated with a fifth selection channel of the second multi-channel selection valve, and controlling the driving mechanism to drive the first injector and the second injector to correspondingly extract 2-8mL of positive quality control sample and sterile pure water;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the second selection channel of the first multi-channel selection valve and controlling the driving mechanism to drive the first injector and the second injector to respectively extract quantitative salt liquid, wherein the extraction volume of the salt liquid is 1/10 of the extraction volume of the sterile pure water;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with a third selection channel of the first multi-channel selection valve and controlling the driving mechanism to push and pull the pull rods of the first injector and the second injector for multiple times, so that the liquid in the injectors is correspondingly pushed to the sample detection pool and the reference detection pool after being fully mixed;
calculating relative luminescence inhibition rate H (%); if the relative luminescence inhibition rate H (%) is more than 20%, judging that the luminescent bacteria in the bacterial liquid are normal; otherwise, replacing the bacterial liquid.
In some embodiments of the present application, in order to provide the analyzer of the present invention with an automatic pipeline cleaning function, an electromagnetic valve and a waste liquid pool are further disposed in the analyzer; the electromagnetic valve comprises two input ports and an output port, the two input ports are respectively used for communicating the water sample to be detected and the sterile pure water, and the output port is communicated with a fifth selection channel of the first multi-channel selection valve; the waste liquid pool is respectively communicated with the liquid outlets of the sample detection pool and the reference detection pool and is used for collecting waste liquid discharged by the sample detection pool and the reference detection pool; when entering the water quality analysis process, the control module controls the electromagnetic valve to communicate the input port of the electromagnetic valve, which is communicated with a water sample to be detected, with the output port of the electromagnetic valve, and executes the following channel cleaning process after the water quality analysis is finished:
controlling the electromagnetic valve to communicate an input port of the electromagnetic valve, which is communicated with the sterile pure water, with an output port of the electromagnetic valve;
controlling the first multichannel selection valve and the second multichannel selection valve to communicate the common channel of the first multichannel selection valve and the second multichannel selection valve with the fifth selection channel of the second multichannel selection valve; the driving mechanism is controlled to drive the first injector and the second injector to respectively extract enough sterile pure water;
and controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the third selection channel of the first multi-channel selection valve and the second multi-channel selection valve, controlling the driving mechanism to drive the first injector and the second injector to correspondingly push sterile pure water in the injectors to the sample detection pool and the reference detection pool for cleaning, and discharging the cleaned waste liquid to the waste liquid pool.
In some embodiments of the present application, the drive mechanism includes a motor, a linkage, and a transmission assembly; the motor runs under the control action of the control module; the connecting rod is connected with the pull rod of the first injector and the pull rod of the second injector; the transmission assembly is arranged on a rotating shaft of the motor, converts the circular motion of the rotating shaft into linear motion, and drives the connecting rod to carry the pull rod to do reciprocating motion. Two injectors are driven by one motor in parallel, and the two channels detect the water sample to be detected and the reference water sample simultaneously, so that errors can be reduced, interference is eliminated, and the reliability of measurement is improved.
In some embodiments of the present application, the reagent storage unit includes a temperature-controlled refrigerator, in which a plurality of reagent bottles for holding different reagents are placed, for holding the bacteria solution, the salt solution and the positive quality control sample. The bacterial liquid directly used for detection is stored in a temperature-controlled refrigerator, so that the biological activity of the luminous bacteria can be ensured to the maximum extent while the bacterial culture procedure is reduced.
In some embodiments of this application, it is preferable to install the reagent bottle that is used for holding the fungus liquid on miniature magnetic stirring device to place the magneton in the bottle for homogenize the fungus liquid, can guarantee the homogeneity of fungus liquid from this.
In some embodiments of the present application, the sample channel, the reference channel and the photodetecting unit are preferably built into a temperature-controlled box to ensure that the environmental temperature during the reaction and detection process meets the survival requirements of the luminescent bacteria.
In some embodiments of the present application, the sample detection cell and the reference detection cell are preferably designed to be hollow and in a horizontal cylindrical shape, and a reflecting mirror is installed on one of the circular end faces of the horizontal cylindrical detection cell for effectively enhancing the luminous intensity of the mixed solution so as to improve the detection sensitivity; the other circular end face of the transverse cylindrical detection cell is used as a light intensity emergent window to be in seamless connection with the circular incident window of the photomultiplier, so that the stability and the effectiveness of detection can be guaranteed.
In some embodiments of the present application, the liquid outlets of the sample detection cell and the reference detection cell are both disposed at the top of the detection cell, and the liquid inlet is disposed at the bottom of the detection cell and correspondingly communicates with the third selection channels of the first multi-channel selection valve and the second multi-channel selection valve. The mixed liquid enters and exits the detection pool in a downward-entering and upward-exiting mode, so that bubbles in the detection pool can be effectively reduced, the interference influence of the bubbles on a detection result is reduced, and the accuracy of water quality monitoring is improved.
Compared with the prior art, the invention has the advantages and positive effects that: the analyzer combines the luminous bacteria detection method with the flow injection technology, adopts a photoelectric detection means, and realizes the online, automatic and efficient monitoring of the comprehensive biotoxicity of the water quality. The automatic sample introduction and detection of the injector and the multi-channel selection valve are controlled by designing the driving mechanism, so that the automation degree of the analyzer is improved, the operation is simplified, the real-time online monitoring on the comprehensive biotoxicity such as heavy metal, organic pollutants, pesticide residues, antibiotics and the like in the environmental water body can be realized, and the device is particularly suitable for being applied to water source places and water plant inlets and outlets and is also suitable for laboratory detection.
Other features and advantages of the present invention will become more apparent from the detailed description of the embodiments of the present invention when taken in conjunction with the accompanying drawings.
Drawings
Fig. 1 is a schematic overall architecture diagram of an embodiment of the water quality comprehensive biotoxicity analyzer based on luminous bacteria.
Detailed Description
The following detailed description of embodiments of the invention refers to the accompanying drawings.
It should be noted that in the description of the present invention, the terms "top", "bottom", "inner", "outer", and the like, which indicate directions or positional relationships, are based on the directions or positional relationships shown in the drawings, which are for convenience of description only, and do not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," "fourth," and "fifth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that in the description of the present invention, the terms "mounted," "connected," and "connected" are to be construed broadly unless otherwise specifically indicated and limited. For example, it may be a fixed connection, a detachable connection or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
Referring to fig. 1, the water quality comprehensive biotoxicity analyzer of the present embodiment mainly includes a reagent storage unit 100, a sample introduction and reaction unit 200, a photoelectric detection unit 300, and the like. The reagent storage unit 100 is used for storing various reagents required to be used in the water quality detection process, such as a saline solution, a bacteria solution cultured with luminescent bacteria, and the like. The sample introduction and reaction unit 200 is used for controlling the sampling, mixing and reaction of a water sample to be tested, a reference water sample and various reagents. The photoelectric detection unit 300 is used for detecting the luminous intensity of the mixed solution, and the detected luminous intensity can be used for analyzing the comprehensive biological toxicity of the water sample to be detected.
In a preferred embodiment, the reagent storage unit 100 mainly includes a temperature-controlled refrigerator 101 and reagent bottles for containing different reagents, such as a reagent bottle 102 for containing saline solution, a reagent bottle 103 for containing bacteria solution, a reagent bottle 104 for containing positive control samples, and the like. The temperature-controlled refrigerating box 101 is preferably controlled by semiconductor refrigeration technology, and the reagent bottles 102, 103 and 104 are arranged in the temperature-controlled refrigerating box and used for providing constant low-temperature storage environment for various reagents in the reagent bottles.
In the embodiment, the temperature in the temperature-controlled refrigerator 101 is preferably controlled to be 4 ℃ ± 3 ℃, and the bacteria liquid directly used for detection is stored in the temperature-controlled refrigerator 101, so that the biological activity of the luminescent bacteria can be ensured to the maximum extent while the culture program of the luminescent bacteria is reduced.
In this embodiment, the reagent bottle 103 for containing the bacteria solution is preferably made of teflon, and the other reagent bottles 102 and 104 may be made of PP. This implementationThe bacterial liquid can be Vibrio fischeri bacterial liquid or photorhabdus brightens bacterial liquid, the salt liquid can be sodium chloride solution with concentration of 22%, and the positive quality control sample can be Zn2+The concentration is 2.0mg/L zinc sulfate solution.
In order to ensure the homogeneity of the bacterial liquid, it is preferable to install a micro-magnetic stirring device 105 on the bottom of the reagent bottle 103 containing the bacterial liquid, and to place a magnet 106 inside the reagent bottle 103. The magnetons 106 move under the magnetic force of the micro magnetic stirring device 105 to stir the bacteria liquid, so that the luminescent bacteria are uniformly distributed in the bacteria liquid.
In addition, the analyzer needs to use sterile pure water as a reference water sample and a pipeline cleaning solution in the working process. The sterile pure water is not required to be stored in the temperature-controlled refrigerating box 101, and is only required to be placed in a normal-temperature environment together with a water sample to be detected.
In some embodiments, the sample and reaction unit 200 comprises a sample channel and a reference channel controlled in parallel. The sample channel is used for automatic sample introduction, mixing and reaction of a water sample to be detected and various reagents; the reference channel is used for automatic sample introduction, mixing and reaction of a reference water sample and various reagents.
As a preferred embodiment, a first multi-channel selection valve 210 and a first syringe 211 are provided in the sample channel. The first multi-channel selection valve 210 is preferably a five-channel selection valve, and comprises five selection channels 1-5 and a common channel c. The first selection channel 1 is preferably communicated with a bacteria liquid reagent bottle 103 through a pipeline and is used for sampling bacteria liquid; the second selection channel 2 is preferably communicated with a saline solution reagent bottle 102 through a pipeline and is used for sampling saline solution; the third selection channel 3 is preferably used as an output channel, is communicated with the photoelectric detection unit 300 through a pipeline, and is used for injecting the mixed liquid into the photoelectric detection unit 300; the fourth selection channel 4 is preferably communicated with a positive quality control sample reagent bottle 104 through a pipeline for sampling positive quality control samples; the fifth selection channel 5 is used for sampling a water sample to be detected; a common channel c is in communication with the first injector 211 via a conduit, and the common channel c may be selectively in communication with the five selection channels 1-5 of the first multi-channel selection valve 210.
In order to provide the analyzer with a self-cleaning function, the present embodiment preferably adds a solenoid valve 213, such as a two-position three-way solenoid valve including two input ports and one output port, to the sample channel as shown in fig. 1. An output port of the two-position three-way electromagnetic valve 213 is communicated with the fifth selection channel 5 of the first multi-channel selection valve 210 through a pipeline, two input ports are respectively communicated with the water sample to be detected and the sterile pure water in a one-to-one correspondence manner, the electromagnetic valve 213 is controlled to select one input port to be communicated with the output port thereof according to different working modes, the water sample to be detected is further provided for the fifth selection channel 5 of the first multi-channel selection valve 210 in the water quality analysis process, and the sterile pure water is provided for the fifth selection channel 5 of the first multi-channel selection valve 210 in the cleaning process.
Likewise, the present embodiment also provides a multi-channel selection valve and an injector in the reference channel, which are defined as a second multi-channel selection valve 220 and a second injector 221, respectively, for the sake of distinction. Wherein, the second multi-channel selection valve 220 also preferably adopts a five-channel selection valve, which comprises five selection channels 1 ' to 5 ' and a common channel c '. The first selection channel 1' is preferably communicated with a bacteria liquid reagent bottle 103 through a pipeline and is used for sampling bacteria liquid; the second selection channel 2' is preferably communicated with a saline solution reagent bottle 102 through a pipeline and is used for sampling saline solution; the third selection channel 3' is preferably used as an output channel, is communicated with the photoelectric detection unit 300 through a pipeline, and is used for injecting the mixed liquid into the photoelectric detection unit 300; the fourth selection channel 4' is preferably communicated with a positive quality control sample reagent bottle 104 through a pipeline for sampling the positive quality control sample; the fifth selection channel 5' is used for sampling a reference water sample (such as sterile pure water); the common channel c 'is communicated with the second injector 221 through a pipe, and the common channel c' may be selectively communicated with the five selection channels 1 'to 5' of the second multi-channel selection valve 220.
In order to realize parallel control of sample introduction and reaction of a water sample to be detected and a reference water sample, in this embodiment, the pull rod 212 of the first injector 211 and the pull rod 222 of the second injector 221 are preferably connected to the same connecting rod 223, and a driving mechanism is designed to push and pull the connecting rod 223 so as to control the pull rods 212 and 222 of the two injectors 211 and 221 to be synchronously pushed and pulled, thereby realizing synchronous and equivalent sampling.
As a preferred embodiment, the driving mechanism is preferably implemented by using a motor and transmission assembly (not shown in the figures) in cooperation with the connecting rod 223. Specifically, the transmission assembly may be mounted on a rotating shaft of the motor, so as to convert a circular motion of the rotating shaft of the motor into a linear motion, and further drive the connecting rod 223 connected thereto to perform a linear motion, thereby implementing a synchronous pushing of the two pull rods 212 and 222. Two injectors are driven by one motor in parallel, and the two channels detect the water sample to be detected and the reference water sample simultaneously, so that errors can be reduced, interference is eliminated, and the reliability of measurement is improved.
In some embodiments, the photo detection unit 300 comprises two detection cells, respectively defined as a sample detection cell 310 and a reference detection cell 320, on each of which a photo multiplier tube, such as a first photo multiplier tube 315 and a second photo multiplier tube 325, is mounted for detecting the intensity of the luminescence of the mixed solution in the detection cell.
As a preferred embodiment, the sample detection cell 310 and the reference detection cell 320 are preferably designed in a hollow cylindrical shape and are disposed in a lateral direction, as shown in FIG. 1. The liquid inlet 311 of the sample detection cell 310 is arranged at the bottom and is connected with the third selection channel 3 of the first multi-channel selection valve 210 through a pipeline; the liquid outlet 312 of the sample detection cell 310 is arranged at the top and is connected with the waste liquid cell 330 through a pipeline; a reflecting mirror 313 is provided on one of the circular end faces of the sample detection cell 310 for reflecting the light irradiated on the reflecting mirror 313 to the other circular end face of the sample detection cell 310; the other circular end face of the sample detection cell 310 is used as a light intensity exit port 314 to be seamlessly connected with the circular entrance window of the first photomultiplier 315.
Similarly, the liquid inlet 321 of the reference detection cell 320 is arranged at the bottom and is connected with the third selection channel 3' of the second multi-channel selection valve 220 through a pipeline; a liquid outlet 322 of the reference detection cell 320 is arranged at the top and is connected with a waste liquid pool 330 through a pipeline; a reflecting mirror 323 is provided on one of the circular end faces of the reference detection cell 320 for reflecting the light irradiated onto the reflecting mirror 323 to the other circular end face of the reference detection cell 320; the other circular end face of the reference detection cell 320 is used as a light intensity exit port 324 to be seamlessly connected with the circular entrance window of the second photomultiplier 325.
This embodiment will detect the inlet setting in bottom in pond, and the liquid outlet setting makes mixed liquid adopt to advance down and go up the mode business turn over detection pond of going out at the top, can effectively reduce the bubble from this, prevents that the bubble from disturbing, improves the measuring degree of accuracy. The detection cell is designed into a cylindrical shape, so that the light intensity emergent port of the detection cell can be in seamless connection with the incident window of the photomultiplier, and the stability and the effectiveness of detection can be ensured. The reflector is additionally arranged on the surface of the detection pool opposite to the light intensity emergent port of the detection pool, so that the light intensity of the luminous bacteria in the mixed liquid can be effectively enhanced, and the measurement sensitivity is improved.
In this embodiment, the sample detecting cell 310, the reference detecting cell 320, the first photomultiplier 315 and the second photomultiplier 325 are preferably disposed in a dark room 340, and the dark room 340 and the sample feeding and reacting unit 200 are preferably disposed in an intelligent micro temperature control chamber 230. By setting the temperature of the intelligent micro temperature control box 230, the reaction temperature can be controlled within 15 ℃ +/-1 ℃.
In order to improve the automation degree of the analyzer, the present embodiment further provides a signal acquisition module and a control module in the analyzer, as shown in fig. 1. The signal acquisition module is connected with the two photomultiplier tubes 315 and 325 and is used for acquiring detection signals which are detected and output by the two photomultiplier tubes 315 and 325 and reflect the luminous intensity of mixed liquid in the detection pool, converting the acquired detection signals into light intensity data and sending the light intensity data to the control module, and carrying out comprehensive biological toxicity analysis on a water sample to be detected in the control module.
Meanwhile, the control module is used as a control core of the whole analyzer and is also responsible for controlling the working states of the motor, the multi-channel selection valves 210 and 220 and the electromagnetic valve 213 so as to realize the automatic execution of a water quality detection process, a cleaning process, a negative detection process and a positive detection process and simplify the operation of personnel.
The control module can be connected to the upper computer 400 through a wired or wireless communication module, on one hand, the control module receives an instruction sent by the upper computer 400 and controls the analyzer to enter a corresponding working mode; on the other hand, the detection result generated by the control module is uploaded to the upper computer 400 so as to facilitate observation of water quality monitoring personnel.
The specific operation of the water quality comprehensive biotoxicity analyzer of the embodiment is described in detail below with reference to fig. 1.
1. Water quality detection process
1.1, a preparation stage;
controlling the electromagnetic valve 213 to communicate the input port (e.g. the normally closed port of the electromagnetic valve 213) of the water sample to be detected with the output port thereof; and starting the magnetic stirring device 105 and continuously stirring the bacteria liquid for 1 minute to uniformly mix the bacteria liquid.
1.2, respectively sampling equivalent bacteria liquid from the control sample channel and the reference channel;
in this embodiment, the control module first controls the two multi-channel selector valves 210 and 220 to correspondingly communicate the common channels c and c 'with the first selector channels 1 and 1', and starts the motors to pull the pull rods 212 and 222 of the syringes 211 and 221 outward, so that the two syringes 211 and 221 respectively suck 0.5-2.5mL of bacteria solution.
1.3, controlling the sample channel and the reference channel to sample a water sample to be detected and sterile pure water in equal amount respectively;
after the bacteria liquid is sampled, the control module switches the common channels c and c 'of the two multi-channel selection valves 210 and 220 to be correspondingly communicated with the fifth selection channels 5 and 5', and starts the motor to continuously pull the pull rods 212 and 222 of the injectors 211 and 221 outwards, so that the two injectors 211 and 221 respectively suck 2-8mL of water samples to be detected or sterile pure water.
1.4, respectively sampling salt solution with the same amount in the control sample channel and the reference channel;
after sampling the water sample, the control module switches the common channels c and c 'of the two multi-channel selection valves 210 and 220 to be correspondingly communicated with the second selection channels 2 and 2', and starts the motors to continuously pull the pull rods 212 and 222 of the injectors 211 and 221 outwards, so that the two injectors 211 and 221 respectively suck one tenth of the volume of the saline solution of the water sample to be detected.
1.5, correspondingly pushing the mixed liquid obtained by sampling the sample channel and the reference channel to a sample detection pool and a reference detection pool;
in this embodiment, the control module controls the motor to drive the pull rods 212 and 222 of the two syringes 211 and 221 to push and pull back and forth 2-10 times, so that the mixed liquid in the two syringes 211 and 221 can be fully mixed. Then, the control module controls the two multi-channel selection valves 210 and 220 to switch the common channels c and c 'to be correspondingly communicated with the third selection channels 3 and 3', and starts the motors to push the pull rods 212 and 222 of the two injectors 211 and 221, so as to correspondingly push the mixed liquid in the two injectors 211 and 221 to the sample detection cell 310 and the reference detection cell 320.
1.6, starting a photomultiplier, and measuring the luminous intensity of the mixed liquid in the sample detection pool and the reference detection pool;
in this embodiment, after the driving motor pushes the mixed liquid in the syringes 211, 221 to the detection cells 310, 320, the control module starts the first photomultiplier 315 and the second photomultiplier 325 to detect the luminous intensity of the mixed liquid in the sample detection cell 310 and the reference detection cell 320, respectively, and starts timing. When the timing reaches the time t, the signal acquisition module acquires detection signals output by the first photomultiplier 315 and the second photomultiplier 325, and converts the detection signals into corresponding light intensity data IstAnd Ict. Wherein t may be set to 5mim, 15mim, or 30 mim; i isstThe luminous intensity of the mixed solution in the sample detection cell 310; i isctThe luminescence intensity of the mixed solution in the detection cell 320 is referred to.
1.7, calculating the relative luminescence inhibition rate, and judging the comprehensive biological toxicity of the water sample to be detected;
in this embodiment, the formula may be utilized:
H（%）=100×（1-Ist/Ict）
and (3) calculating the relative luminescence inhibition rate after the reaction time t, and further judging the comprehensive biological toxicity of the water sample to be detected according to the relative luminescence inhibition rate H (%).
Regarding the relative luminescence inhibition, the calculation methods described in national Standard GBT 15441-1995 and International Standard ISO11348-3The method needs to measure the initial luminous intensity and calculate the correction factor, and is complicated. This example is simplified on the basis of the related documents, that is, a certain volume of bacteria liquid in the same growth state is taken simultaneously to react with a water sample to be tested and a blank reference sample (sterile pure water) in the same volume, and the luminous intensity I at the reaction time t is recordedstAnd IctUsing the formula H (%) =100 (1-I)st/Ict) The calculation is performed so that the calculation process of the relative light emission suppression ratio is simplified.
The determination of the comprehensive biotoxicity of water quality by using the relative luminescence inhibition rate is the prior art, and the embodiment is not described herein.
2. Channel cleaning process
2.1, a preparation stage;
the electromagnetic valve 213 is controlled to communicate its input port (e.g., the normally open port of the electromagnetic valve 213) for communicating sterile pure water with its output port.
2.2, controlling the sample channel and the reference channel to respectively extract sufficient sterile pure water;
in this embodiment, the first multi-channel selector valve 210 and the second multi-channel selector valve 220 may be first controlled to communicate their common channels c, c 'with their own fifth selector channels 5, 5', respectively; then, the motor is activated to pull the pull rods 212, 222 of the syringes 211, 221 outward, so that both syringes 211, 221 respectively suck a sufficient amount of sterile pure water, for example, to fill the entire syringes.
2.3, correspondingly pushing sterile pure water in the sample channel and the reference channel to the sample detection pool and the reference detection pool;
in the present embodiment, the control module controls the two multi-channel selection valves 210, 220 to switch the common channels c, c 'to be correspondingly communicated with the third selection channels 3, 3' of the two multi-channel selection valves, and starts the motors to push the pull rods 212, 222 of the two syringes 211, 221, so as to correspondingly push the sterile pure water in the two syringes 211, 221 to the sample detection cell 310 and the reference detection cell 320 for washing.
2.4, discharging the cleaned waste liquid;
in this embodiment, a suction pump may be disposed in the waste liquid tank 330 to draw the waste liquid after washing in the sample detection tank 310 and the reference detection tank 320 and collect the waste liquid in the waste liquid tank 330.
3. Negative detection process
To ensure the parallelism of the measurements, it is necessary to periodically initiate a negative test on the analyzer, preferably once every 10 samples tested.
3.1, a preparation stage;
the control electromagnetic valve 213 communicates the input port of the sterile pure water with the output port thereof; and starting the magnetic stirring device 105 and continuously stirring the bacteria liquid for 1 minute to uniformly mix the bacteria liquid.
3.2, respectively sampling equivalent bacteria liquid from the control sample channel and the reference channel;
in this embodiment, the control module first controls the two multi-channel selector valves 210 and 220 to correspondingly communicate the common channels c and c 'with the first selector channels 1 and 1', and starts the motors to pull the pull rods 212 and 222 of the syringes 211 and 221 outward, so that the two syringes 211 and 221 respectively suck 0.5-2.5mL of bacteria solution.
3.3, controlling the sample channel and the reference channel to respectively extract the same amount of sterile pure water;
after sampling the bacteria liquid, the control module switches the common channels c and c 'of the two multi-channel selection valves 210 and 220 to be correspondingly communicated with the fifth selection channels 5 and 5', starts the motor to continuously pull the pull rods 212 and 222 of the injectors 211 and 221 outwards, so that the two injectors 211 and 221 respectively suck 2-8mL of sterile pure water.
3.4, respectively sampling the same amount of salt solution from the control sample channel and the reference channel;
after the sterile pure water is extracted, the control module switches the common channels c and c 'of the two multi-channel selection valves 210 and 220 to be correspondingly communicated with the second selection channels 2 and 2', and starts the motors to continuously pull the pull rods 212 and 222 of the injectors 211 and 221 outwards, so that the two injectors 211 and 221 respectively suck the saline solution with the volume of one tenth of the volume of the sterile pure water.
3.5, correspondingly pushing the mixed liquid obtained by sampling the sample channel and the reference channel to the sample detection pool and the reference detection pool;
in this embodiment, the control module controls the motor to drive the pull rods 212 and 222 of the two syringes 211 and 221 to push and pull back and forth 2-10 times, so that the mixed liquid in the two syringes 211 and 221 can be fully mixed. Then, the control module controls the two multi-channel selection valves 210 and 220 to switch the common channels c and c 'to be correspondingly communicated with the third selection channels 3 and 3', and starts the motors to push the pull rods 212 and 222 of the two injectors 211 and 221, so as to correspondingly push the mixed liquid in the two injectors 211 and 221 to the sample detection cell 310 and the reference detection cell 320.
3.6, starting the photomultiplier, and measuring the luminous intensity of the mixed liquid in the sample detection pool and the reference detection pool;
in this embodiment, after the driving motor pushes the mixed liquid in the syringes 211, 221 to the detection cells 310, 320, the control module starts the first photomultiplier 315 and the second photomultiplier 325 to detect the luminous intensity of the mixed liquid in the sample detection cell 310 and the reference detection cell 320, respectively, and starts timing. When the timing reaches the time t, the signal acquisition module acquires detection signals output by the first photomultiplier 315 and the second photomultiplier 325, and converts the detection signals into corresponding light intensity data Ist’And Ict’。
3.7, calculating the relative luminescence inhibition rate;
using the formula:
H（%）=100×（1-Ist’/Ict’）
calculating the relative luminescence inhibition rate after the reaction time t, and if the relative luminescence inhibition rate H (%) is within +/-10%, judging that the luminescent bacteria in the bacterial liquid are normal and the parallelism of the analyzer meets the requirement; otherwise, the bacteria liquid needs to be replaced or whether the analyzer has a fault is detected.
4. Positive detection procedure
In order to ensure the effectiveness of the bacterial fluid, the analyzer needs to be periodically activated for negative detection, and preferably, positive detection is performed every 10 samples.
4.1, a preparation stage;
and starting the magnetic stirring device 105 and continuously stirring the bacteria liquid for 1 minute to uniformly mix the bacteria liquid.
4.2, respectively sampling equivalent bacteria liquid from the control sample channel and the reference channel;
in this embodiment, the control module first controls the two multi-channel selector valves 210 and 220 to correspondingly communicate the common channels c and c 'with the first selector channels 1 and 1', and starts the motors to pull the pull rods 212 and 222 of the syringes 211 and 221 outward, so that the two syringes 211 and 221 respectively suck 0.5-2.5mL of bacteria solution.
4.3, respectively sampling the same amount of positive property control sample and sterile pure water by the control sample channel and the reference channel;
after the bacteria liquid is extracted, the control module controls the first multi-channel selection valve 210 to communicate the common channel c with the fourth selection channel 4 of the first multi-channel selection valve, and controls the second multi-channel selection valve 220 to communicate the common channel c 'with the fifth selection channel 5' of the second multi-channel selection valve; then, the motor is started to pull the pull rods 212 and 222 of the syringes 211 and 221 outwards continuously, so that the first syringe 211 extracts 2-8mL of the positive quality control sample, and the second syringe 221 extracts 2-8mL of sterile pure water.
4.4, respectively sampling the same amount of salt solution from the control sample channel and the reference channel;
after the positive quality control sample is extracted, the control module switches the common channels c and c 'of the two multi-channel selection valves 210 and 220 to be correspondingly communicated with the second selection channels 2 and 2', and starts the motor to continuously pull the pull rods 212 and 222 of the injectors 211 and 221 outwards so that the two injectors 211 and 221 respectively suck the saline solution with one tenth volume of the positive quality control sample.
4.5, correspondingly pushing the mixed liquid obtained by sampling the sample channel and the reference channel to the sample detection pool and the reference detection pool;
in this embodiment, the control module controls the motor to drive the pull rods 212 and 222 of the two syringes 211 and 221 to push and pull back and forth 2-10 times, so that the mixed liquid in the two syringes 211 and 221 can be fully mixed. Then, the control module controls the two multi-channel selection valves 210 and 220 to switch the common channels c and c 'to be correspondingly communicated with the third selection channels 3 and 3', and starts the motors to push the pull rods 212 and 222 of the two injectors 211 and 221, so as to correspondingly push the mixed liquid in the two injectors 211 and 221 to the sample detection cell 310 and the reference detection cell 320.
4.6, starting the photomultiplier, and measuring the luminous intensity of the mixed liquid in the sample detection pool and the reference detection pool;
in this embodiment, after the driving motor pushes the mixed liquid in the syringes 211, 221 to the detection cells 310, 320, the control module starts the first photomultiplier 315 and the second photomultiplier 325 to detect the luminous intensity of the mixed liquid in the sample detection cell 310 and the reference detection cell 320, respectively, and starts timing. When the timing reaches the time t, the signal acquisition module acquires detection signals output by the first photomultiplier 315 and the second photomultiplier 325, and converts the detection signals into corresponding light intensity data Ist’’And Ict’’。
4.7, calculating the relative luminescence inhibition rate;
using the formula:
H（%）=100×（1-Ist’’/Ict’’）
calculating the relative luminescence inhibition rate after the reaction time t, and if the relative luminescence inhibition rate H (%) is more than 20%, judging that the luminescent bacteria in the bacterial liquid are normal; otherwise, the bacteria liquid needs to be replaced.
Of course, in the positive detection process, the 4.3 step can be replaced by: the first multi-channel selection valve 210 is controlled to communicate the common channel c with the fifth selection channel 5 of the first multi-channel selection valve, and the electromagnetic valve 213 is controlled to communicate the input port of the first multi-channel selection valve, which is communicated with the sterile pure water, with the output port of the first multi-channel selection valve; controlling the second multi-channel selector valve 220 to communicate its common channel c 'with its own fourth selector channel 4'; then, the motor is started to pull the pull rods 212 and 222 of the syringes 211 and 221 outwards continuously, so that the first syringe 211 extracts 2-8mL of sterile pure water, and the second syringe 221 extracts 2-8mL of positive quality control sample. Thus, after the relative luminescence inhibition rate is calculated, if the relative luminescence inhibition rate H (%) is less than-20%, the luminescent bacteria in the bacterial solution is judged to be normal; otherwise, the bacteria liquid needs to be replaced.
The embodiment combines the luminous bacteria detection method with the flow injection, and the obtained analyzer is small in size, small in bacterial liquid consumption, high in automation degree and capable of improving monitoring efficiency. Negative detection and positive detection are specially set in the process, so that the missing report and the false report can be avoided.
Of course, the above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A water quality comprehensive biotoxicity analyzer based on luminous bacteria is characterized by comprising:
a reagent storage unit which stores a salt solution and a bacterial solution in which luminous bacteria are cultured;
a sample channel comprising a first multi-channel selection valve and a first syringe; a first selection channel of the first multi-channel selection valve is communicated with the bacterial liquid, a second selection channel is communicated with the salt liquid, a fifth selection channel is used for being communicated with a water sample to be detected, and a common channel is communicated with the first injector;
a reference channel comprising a second multi-channel selection valve and a second injector; a first selection channel of the second multi-channel selection valve is communicated with the bacterial liquid, a second selection channel is communicated with the salt liquid, a fifth selection channel is used for being communicated with sterile pure water, and a common channel is communicated with the second injector;
a photoelectric detection unit comprising a sample detection cell and a reference detection cell; the sample detection cell is communicated with a third selection channel of the first multi-channel selection valve, and the reference detection cell is communicated with a third selection channel of the second multi-channel selection valve; a first photomultiplier is arranged on the sample detection pool and is used for detecting the luminous intensity of the mixed liquid in the sample detection pool; a second photomultiplier is arranged on the reference detection pool and is used for detecting the luminous intensity of the mixed liquid in the reference detection pool;
a drive mechanism connecting the pull rods of the first and second syringes; in the water quality analysis process, the driving mechanism drives the pull rod to synchronously control the first injector and the second injector to correspondingly extract equal amounts of bacteria liquid, equal amounts of salt liquid, equal amounts of water samples to be detected and sterile pure water through the first multi-channel selection valve and the second multi-channel selection valve one by one, and after the first injector and the second injector are mixed, the pull rod is pushed to correspondingly push mixed liquid to the sample detection pool and the reference detection pool through third selection channels of the first multi-channel selection valve and the second multi-channel selection valve respectively.
2. The comprehensive water quality biotoxicity analyzer based on luminous bacteria as claimed in claim 1, further comprising:
the signal acquisition module is connected with the first photomultiplier and the second photomultiplier and is used for converting detection signals which are output by the first photomultiplier and the second photomultiplier and reflect the luminous intensity of the mixed liquid into light intensity data;
and the control module is used for controlling the driving mechanism, the first multi-channel selection valve and the second multi-channel selection valve and receiving the light intensity data output by the signal acquisition module so as to be used for analyzing the comprehensive biological toxicity of the water sample to be detected.
3. The comprehensive water quality biotoxicity analyzer based on luminous bacteria of claim 2, wherein in the water quality analysis process, the control module executes the following water quality detection process:
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the first selection channel of the first multi-channel selection valve, and controlling the driving mechanism to drive the first injector and the second injector to respectively extract 0.5-2.5mL of bacterial liquid;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with a fifth selection channel of the first multi-channel selection valve and controlling the driving mechanism to drive the first injector and the second injector to correspondingly extract 2-8mL of water sample to be detected and sterile pure water;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the second selection channel of the first multi-channel selection valve and controlling the driving mechanism to drive the first injector and the second injector to respectively extract quantitative salt liquid, wherein the extraction volume of the salt liquid is 1/10 of the extraction volume of the water sample to be detected;
and controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the third selection channel of the first multi-channel selection valve and controlling the driving mechanism to push and pull the pull rods of the first injector and the second injector for multiple times, so that the liquid in the injectors is correspondingly pushed to the sample detection pool and the reference detection pool after being fully mixed.
4. The water quality comprehensive biotoxicity analyzer based on luminous bacteria as claimed in claim 3, wherein in the water quality detection process, after the mixed liquid is pushed to the sample detection pool and the reference detection pool, the control module starts the first photomultiplier and the second photomultiplier and starts timing, the signal acquisition module acquires the luminous intensity of the mixed liquid detected by the first photomultiplier and the second photomultiplier when the timing reaches t, and the formula H (%) =100 × (1-I) is utilizedst/Ict) Calculating the relative luminescence inhibition rate after the reaction time t, and judging the comprehensive biological toxicity of the water sample to be detected according to the relative luminescence inhibition rate; wherein, IstThe luminous intensity of the mixed liquid in the sample detection pool is obtained; i isctThe luminous intensity of the mixed liquid in the detection pool is taken as reference.
5. The photobacteria-based water quality synthetic biotoxicity analyzer according to claim 4, wherein the control module periodically initiates a negative detection process:
replacing the water sample to be detected with sterile pure water, repeatedly executing the water quality detection process, and calculating the relative luminescence inhibition rate H (%);
if the relative luminescence inhibition rate H (%) is within +/-10%, judging that the luminescent bacteria in the bacterial liquid are normal and the parallelism of an analyzer meets the requirement; otherwise, replacing the bacteria liquid or detecting whether the analyzer has a fault.
6. The comprehensive water quality biotoxicity analyzer based on luminous bacteria as claimed in claim 4, wherein,
a positive property control sample is also stored in the reagent storage unit;
the first multi-channel selection valve and the second multi-channel selection valve are respectively communicated with the positive property control sample through a fourth selection channel of the first multi-channel selection valve and the second multi-channel selection valve;
the control module periodically initiates a positive detection process:
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the first selection channel of the first multi-channel selection valve, and controlling the driving mechanism to drive the first injector and the second injector to respectively extract 0.5-2.5mL of bacterial liquid;
controlling a common channel of the first multi-channel selection valve to be communicated with a fourth selection channel of the first multi-channel selection valve, controlling a common channel of the second multi-channel selection valve to be communicated with a fifth selection channel of the second multi-channel selection valve, and controlling the driving mechanism to drive the first injector and the second injector to correspondingly extract 2-8mL of positive quality control sample and sterile pure water;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the second selection channel of the first multi-channel selection valve and controlling the driving mechanism to drive the first injector and the second injector to respectively extract quantitative salt liquid, wherein the extraction volume of the salt liquid is 1/10 of the extraction volume of the sterile pure water;
controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with a third selection channel of the first multi-channel selection valve and controlling the driving mechanism to push and pull the pull rods of the first injector and the second injector for multiple times, so that the liquid in the injectors is correspondingly pushed to the sample detection pool and the reference detection pool after being fully mixed;
calculating relative luminescence inhibition rate H (%); if the relative luminescence inhibition rate H (%) is more than 20%, judging that the luminescent bacteria in the bacterial liquid are normal; otherwise, replacing the bacterial liquid.
7. The apparatus for comprehensive water quality biotoxicity analysis based on luminescent bacteria according to any one of claims 2 to 6, further comprising:
the electromagnetic valve comprises two input ports and an output port, the two input ports are respectively used for communicating the water sample to be detected and the sterile pure water, and the output port is communicated with a fifth selection channel of the first multi-channel selection valve;
the waste liquid pool is respectively communicated with the liquid outlets of the sample detection pool and the reference detection pool and is used for collecting waste liquid discharged by the sample detection pool and the reference detection pool;
when the control module enters a water quality analysis process, the electromagnetic valve is controlled to communicate the input port of the electromagnetic valve, which is communicated with a water sample to be detected, with the output port of the electromagnetic valve, and the following channel cleaning process is executed after the water quality analysis is finished:
controlling the electromagnetic valve to communicate an input port of the electromagnetic valve, which is communicated with the sterile pure water, with an output port of the electromagnetic valve;
controlling the first multichannel selection valve and the second multichannel selection valve to communicate the common channel of the first multichannel selection valve and the second multichannel selection valve with the fifth selection channel of the second multichannel selection valve; the driving mechanism is controlled to drive the first injector and the second injector to respectively extract enough sterile pure water;
and controlling the first multi-channel selection valve and the second multi-channel selection valve to communicate the common channel with the third selection channel of the first multi-channel selection valve and the second multi-channel selection valve, controlling the driving mechanism to drive the first injector and the second injector to correspondingly push sterile pure water in the injectors to the sample detection pool and the reference detection pool for cleaning, and discharging the cleaned waste liquid to the waste liquid pool.
8. The photobacteria-based water quality synthetic biotoxicity analyzer according to any one of claims 2 to 6, wherein the driving mechanism includes:
the motor runs under the control action of the control module;
a connecting rod connecting the pull rod of the first syringe and the pull rod of the second syringe;
and the transmission assembly is arranged on a rotating shaft of the motor, converts the circular motion of the rotating shaft into linear motion and drives the connecting rod to carry the pull rod to do reciprocating motion.
9. The comprehensive water quality biotoxicity analyzer based on luminous bacteria according to any one of claims 1 to 6,
the reagent storage unit comprises a temperature-controlled refrigerating box, wherein a plurality of reagent bottles for containing different reagents are placed in the temperature-controlled refrigerating box and are used for containing the bacteria liquid and the salt liquid; the reagent bottle for containing the bacteria liquid is arranged on the miniature magnetic stirring device, and magnetons are placed in the bottle for uniformly stirring the bacteria liquid;
the sample channel, the reference channel and the photoelectric detection unit are arranged in the temperature control box.
10. The water quality comprehensive biotoxicity analyzer based on luminous bacteria as claimed in any one of claims 1 to 6, wherein the sample detection cell and the reference detection cell are both hollow horizontal cylinders, a reflector is mounted on one circular end face of the horizontal cylinder detection cell, and the other circular end face is used as a light intensity exit window to be in seamless connection with a circular entrance window of the photomultiplier; the top surface of the horizontal cylindrical detection pool is provided with a liquid outlet, and the bottom surface is provided with a liquid inlet.
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| CN202010646641.XA CN111707659A (en) | 2020-07-07 | 2020-07-07 | Luminous bacteria-based water quality comprehensive biotoxicity analyzer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113267488A (en) * | 2021-06-02 | 2021-08-17 | 上海泽安实业有限公司 | Automatic detection equipment and detection method for toxicity of water sample |
| CN113917097A (en) * | 2021-09-27 | 2022-01-11 | 聚光科技(杭州)股份有限公司 | Method for detecting comprehensive toxicity of water sample |
| CN115046922A (en) * | 2022-03-31 | 2022-09-13 | 重庆大学 | Portable water quality comprehensive toxicity analyzer based on luminous bacteria and detection method |
-
2020
- 2020-07-07 CN CN202010646641.XA patent/CN111707659A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113267488A (en) * | 2021-06-02 | 2021-08-17 | 上海泽安实业有限公司 | Automatic detection equipment and detection method for toxicity of water sample |
| CN113917097A (en) * | 2021-09-27 | 2022-01-11 | 聚光科技(杭州)股份有限公司 | Method for detecting comprehensive toxicity of water sample |
| CN115046922A (en) * | 2022-03-31 | 2022-09-13 | 重庆大学 | Portable water quality comprehensive toxicity analyzer based on luminous bacteria and detection method |
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