CN212780531U - Micro-fluidic chemiluminescence detection device - Google Patents
Micro-fluidic chemiluminescence detection device Download PDFInfo
- Publication number
- CN212780531U CN212780531U CN202021874014.3U CN202021874014U CN212780531U CN 212780531 U CN212780531 U CN 212780531U CN 202021874014 U CN202021874014 U CN 202021874014U CN 212780531 U CN212780531 U CN 212780531U
- Authority
- CN
- China
- Prior art keywords
- micro
- reaction
- water sample
- chemiluminescence
- microfluidic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002038 chemiluminescence detection Methods 0.000 title claims abstract description 34
- 238000006243 chemical reaction Methods 0.000 claims abstract description 75
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 71
- 238000001514 detection method Methods 0.000 claims abstract description 46
- 238000004020 luminiscence type Methods 0.000 claims abstract description 12
- 238000012544 monitoring process Methods 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 17
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 claims description 16
- 239000000725 suspension Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 10
- 230000002572 peristaltic effect Effects 0.000 claims description 9
- 238000005842 biochemical reaction Methods 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000002699 waste material Substances 0.000 claims description 5
- 238000005452 bending Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 description 24
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
- 229910052760 oxygen Inorganic materials 0.000 description 12
- 239000001301 oxygen Substances 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 239000005416 organic matter Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 239000012530 fluid Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 239000010802 sludge Substances 0.000 description 5
- 238000005273 aeration Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- RVJVDCVIJCBUTH-UHFFFAOYSA-M sodium;5-amino-2h-phthalazin-3-ide-1,4-dione Chemical class [Na+].O=C1N[N-]C(=O)C2=C1C(N)=CC=C2 RVJVDCVIJCBUTH-UHFFFAOYSA-M 0.000 description 4
- 238000003113 dilution method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000036284 oxygen consumption Effects 0.000 description 2
- -1 potassium ferricyanide Chemical compound 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 229930003448 Vitamin K Natural products 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000004053 quinones Chemical class 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000010223 real-time analysis Methods 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model provides a micro-fluidic chemiluminescence detection device, wherein the device comprises a chemiluminescence detection system and a micro-fluidic system arranged in the chemiluminescence detection system; the system comprises a micro-fluidic system, a water sample to be detected and a reaction detection object, wherein the micro-fluidic system comprises a micro-fluidic chip, the water sample to be detected is connected with the micro-fluidic chip, and the reaction detection object is connected with the micro-fluidic chip and corresponds to the water sample to be detected; the water sample to be detected and the reaction detection object are injected into the micro-fluidic chip, and react in the micro-fluidic chip to generate a luminous signal; the chemiluminescence detection system is used for monitoring a luminescence signal in real time so as to realize online BOD detection of a water sample to be detected. Utilize above-mentioned utility model can realize the online real-time of water sampleBOD5Monitoring, fast detection speed and high quality.
Description
Technical Field
The utility model relates to a water biochemical oxygen demand measures technical field, more specifically relates to a micro-fluidic chemiluminescence detection device.
Background
Organic substances are one of the important pollutants in water body and can be catalyzed by microorganismsIs oxidized by oxygen, leads to the anoxic condition of the water body, and further leads to the death of aquatic organisms to cause the putrefaction of the water body. Therefore, Biochemical Oxygen Demand (BOD) is an important index for evaluating organic matter pollution in water, and since oxygen consumption in natural water is a slow process, measuring the total BOD amount requires tens of days or even hundreds of days, and operation is inconvenient. Thus researchers have measured water quality as BOD over a period of time, including five days of biochemical oxygen demand BOD5Seven day chemical oxygen demand BOD7And correspondingly BOD10And BOD20And the like. The standard widely used at present is BOD5。
Existing BOD5The measuring method mainly comprises an inoculation dilution method, a temperature rising method, an activated sludge aeration degradation method, a biochemical sensor method and the like. Among them, the inoculation dilution method, which is a standard measurement method, requires a constant temperature (20 ℃) and airtightness at all times for 5-day measurement although its accuracy is high, and is not convenient for rapid real-time detection. In addition, although the temperature rise method can accelerate the reaction process to a certain extent, BOD at 20 ℃ can be estimated from BOD of 2-3 days5But still for a longer time. The activated sludge aeration degradation method mainly utilizes excessive activated sludge and continuously introduced oxygen to rapidly decompose organic matters in a sample, then measures the chemical oxygen demand (the chemical oxygen demand of the sample without activated sludge aeration: CODi, the chemical oxygen demand of the sample after activated sludge aeration: CODf) before and after the reaction of the sample, and calculates the biochemical oxygen demand (BOD ═ CODi-CODf), but the method is complex to operate and takes about 2 hours.
In addition, the biochemical sensor method is a relatively simple and fast method, which mainly converts the oxidation process of organic matters into photoelectric signals through the design of a series of chemical systems: typical methods include bioluminescence, microbial fuel cell, and biochemical reactor. At present, although these methods lay an important foundation for rapidly estimating the BOD of water quality, further development is still needed to realize convenient and rapid analysis, and provide a powerful tool for real-time monitoring of water samples.
However, the above-mentioned biochemical sensor methods require a large-scale mass spectrometer and are inconvenient to operate; the fluorescence analysis needs an external laser system, the structure is complex, and the stability (light intensity, wavelength and the like) of a light source influences the analysis process; in addition, electrochemical analysis requires an external electrical device, and instability such as electrode contamination can also affect analysis results.
SUMMERY OF THE UTILITY MODEL
In view of the above problem, the utility model aims at providing a micro-fluidic chemiluminescence detection device to solve present water sample BOD and detect cycle length, with high costs, the poor scheduling problem of effect that exists.
The utility model provides a micro-fluidic chemiluminescence detection device, which comprises a chemiluminescence detection system and a micro-fluidic system arranged in the chemiluminescence detection system; the system comprises a micro-fluidic system, a water sample to be detected and a reaction detection object, wherein the micro-fluidic system comprises a micro-fluidic chip, the water sample to be detected is connected with the micro-fluidic chip, and the reaction detection object is connected with the micro-fluidic chip and corresponds to the water sample to be detected; the water sample to be detected and the reaction detection object are injected into the micro-fluidic chip, and react in the micro-fluidic chip to generate a luminous signal; the chemiluminescence detection system is used for monitoring a luminescence signal in real time so as to realize online BOD detection of a water sample to be detected.
In addition, it is a preferred feature that the reaction detector comprises a yeast suspension and a luminol reaction system solution.
In addition, the preferable structural characteristics are that the microfluidic chip comprises a reaction area and a chemiluminescence area which are mutually communicated; simultaneously injecting a water sample to be detected and the yeast suspension into a reaction area, mixing and fully reacting the water sample and the yeast suspension, and inputting the mixture into a chemiluminescence area; and injecting the luminol reaction system solution into the chemiluminescence area and carrying out chemical reaction with the mixed solution of the water sample to be detected and the yeast suspension to generate a luminescence signal.
In addition, the preferred structural feature is that the reaction zone comprises a pipeline of tesla-like valve construction; the pipeline comprises at least two mixing parts and a bending part for connecting the two adjacent mixing parts; the mixing part comprises regularly distributed shunting nodes and collision mixing nodes.
In addition, the preferable structural characteristics are that the chemiluminescence detection system comprises a darkroom, a photomultiplier and a miniature temperature control device; the micro-fluidic chip is placed in a dark room, the photomultiplier is arranged below the chemiluminescence area, and the micro temperature control device is arranged below the reaction area.
In addition, the preferable structural characteristics are that the chemiluminescence detection system comprises a darkroom, a photomultiplier and a miniature temperature control device; the micro-fluidic chip is placed in a dark room, the photomultiplier is arranged below the chemiluminescence area, and the micro temperature control device is arranged below the reaction area.
Furthermore, a preferred structural feature is that the chemiluminescent region comprises a helical channel; and mixing the solution of the luminol reaction system and the mixed solution in the spiral channel and carrying out biochemical reaction.
In addition, the preferable structural feature is that the microfluidic chip includes three inlets and one outlet; and the reaction detection substance and the water sample to be detected are respectively injected into the micro-fluidic chip from the corresponding inlets under the action of the peristaltic pump, and the waste liquid after detection is discharged through the outlet.
In addition, the preferable structural feature is that the reaction detector is injected into the microfluidic chip by a peristaltic pump at a preset flow rate.
By utilizing the micro-fluidic chemiluminescence detection device, a water sample to be detected and a reaction detection object are injected into the micro-fluidic chip, a reaction is carried out in the micro-fluidic chip and a luminescence signal is generated, then the luminescence signal is monitored in real time through the chemiluminescence detection system, and finally, the online BOD detection of the water sample to be detected is realized, so that the rapid and efficient BOD detection can be realized.
To the accomplishment of the foregoing and related ends, one or more aspects of the invention comprise the features hereinafter fully described. The following description and the annexed drawings set forth in detail certain illustrative aspects of the invention. These aspects are indicative, however, of but a few of the various ways in which the principles of the invention may be employed. Moreover, the present invention is intended to include all such aspects and their equivalents.
Drawings
Other objects and results of the invention will be more apparent and readily appreciated by reference to the following description taken in conjunction with the accompanying drawings, and as the invention is more fully understood. In the drawings:
fig. 1 is a schematic structural diagram of a microfluidic chemiluminescence detection apparatus according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a microfluidic chip according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a reaction region according to an embodiment of the present invention.
Wherein the reference numerals include: the kit comprises a microfluidic system 1, a chemiluminescence detection system 2, a microfluidic chip 3, a reaction area 4, a chemiluminescence area 5, a peristaltic pump 6, a water sample to be detected 7, a yeast suspension 8, a luminol reaction system solution 9 and waste liquid 10.
The same reference numbers in all figures indicate similar or corresponding features or functions.
Detailed Description
In the following description, for purposes of explanation, numerous specific details are set forth in order to provide a thorough understanding of one or more embodiments. It may be evident, however, that such embodiment(s) may be practiced without these specific details. In other instances, well-known structures and devices are shown in block diagram form in order to facilitate describing one or more embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
For the purpose of describing the present invention in detail, specific embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Fig. 1 shows a schematic structure of a microfluidic chemiluminescent detection device according to an embodiment of the present invention.
As shown in fig. 1, the microfluidic chemiluminescence detection apparatus according to the embodiment of the present invention includes a chemiluminescence detection system 2 and a microfluidic system 1 disposed in the chemiluminescence detection system 2; the microfluidic system 1 comprises a microfluidic chip 3, a water sample 7 to be detected and a reaction detector, wherein the water sample 7 to be detected is connected with the microfluidic chip 3, and the reaction detector is connected with the microfluidic chip 3 and corresponds to the water sample 7 to be detected; the water sample 7 to be detected and the reaction detection object are both injected into the micro-fluidic chip 3 and react in the micro-fluidic chip 3 to generate a luminous signal; the chemiluminescence detection system 2 is used for monitoring a luminescence signal in real time so as to realize online BOD detection of a water sample 7 to be detected, and has high detection speed and high accuracy.
Specifically, the reaction detector comprises mainly yeast suspension 8 and luminol reaction system solution 9. Wherein yeast suspension 8 may include excess active saccharomyces cerevisiae and quinones for acting as redox mediators; wherein the quinone substances include 1, 2-naphthoquinone-4-sodium sulfonate or vitamin K, and the solution can be physiological saline solution; the luminol reaction system solution 9 comprises excess luminol sodium salt and ferricyanide; the preparation process of the luminol reaction system solution 9 can adopt the following steps: and (3) preparing the luminol sodium salt stock solution, placing the luminol sodium salt stock solution at 4 ℃ in a dark place for 2 days to ensure that the property of the reagent is stable, and diluting and mixing the luminol sodium salt stock solution with a potassium ferricyanide solution when the reagent is used.
In addition, the yeast in the yeast suspension 8 is a preferred microbial strain, and a more targeted combination of strains can be selected according to the specific situation of the local water sample to be detected in the actual operation. In the detection process, the yeast suspension 8 is continuously stirred, and the yeast is uniformly dispersed in the water phase, so that the detection precision of BOD of the water sample to be detected is improved.
Further, the microfluidic chip 3 comprises a reaction region 4 and a chemiluminescence region 5 which are mutually communicated; wherein, a water sample 7 to be tested and a yeast suspension 8 are simultaneously injected into the reaction area 4, mixed and fully reacted, and then input into the chemiluminescence area 5; the luminol reaction system solution 9 is injected into the chemiluminescence region 5 and reacts with the mixed solution to generate a corresponding luminescence signal for the chemiluminescence detection system 2 to collect.
Specifically, fig. 2 and fig. 3 respectively show schematic structures of a microfluidic chip and a reaction region according to an embodiment of the present invention.
As shown in fig. 1 to 3, the microfluidic chip 3 includes a reaction region 4 and a chemiluminescence region 5 connected to each other, the reaction region 4 includes two inlets, and the chemiluminescence region 5 includes one inlet and one outlet; the reaction region 4 further includes a pipeline (hereinafter, referred to as a pipeline or a channel) of a tesla-like valve structure, the pipeline includes at least two mixing portions and a bending portion connecting two adjacent mixing portions, and the mixing portions include regularly distributed flow dividing nodes and colliding mixing nodes; as shown in FIG. 3, the reaction area 4 in the microfluidic chip adopts a pipeline structure similar to a Tesla valve, and the liquid flow (the direction is shown by a dotted line in FIG. 3) can realize the process of multiple shunting-colliding and converging in the mixing part, so that continuous stirring is realized, and the reactants can be ensured to fully react under the action of excessive yeast cells.
In addition, the chemiluminescence region 5 can be arranged in a spiral or other channel structure form which can realize rapid mixing of liquid under the condition of saving space.
Therefore, the micro-fluidic chip 3 comprises three inlets and one outlet, the reaction detection object and the water sample 7 to be detected are respectively injected into the micro-fluidic chip 3 from the two inlets of the reaction area 4 under the action of the peristaltic pump 6, and the reaction detection object and the water sample are subjected to constant-temperature biochemical reaction in the next pipeline to form a mixed solution; after reacting for a certain time, the mixed solution meets the luminol reaction system solution 9 at the inlet of the chemiluminescence region 5, rapid mixing and reaction are completed in the spiral channel, a luminescence signal is generated, and the waste liquid 10 after reaction and detection is discharged from the outlet of the chemiluminescence region 5.
It should be noted that the structure, length, size and flow rate of the above-mentioned pipe or spiral channel can be set and adjusted according to the water sample to be detected or the detection requirement, and are not limited to the specific size and structure shown in the drawings.
In one embodiment of the present invention, the chemiluminescence detection system 2 comprises a darkroom, a photomultiplier tube (not shown in the figure), and a micro temperature control device (not shown in the figure); wherein, the micro-fluidic chip 3 is placed in a darkroom; the micro temperature control device is arranged below the reaction area 4 and used for regulating and controlling the temperature of biochemical reaction, for example, 20 ℃, and the temperature can be optimized and adjusted according to experimental requirements so as to assist the microfluidic system 1 to realize the biochemical reaction; the photomultiplier is arranged below the chemiluminescence region 5 and is used for dynamically and accurately recording luminescence information, namely luminescence signals, in the reaction process in real time.
In addition, in order to ensure the detection precision of the water sample 7 to be detected, the method further comprises the steps of pretreating and evaluating the water sample 7 to be detected before the water sample 7 to be detected is injected into the microfluidic chip 3, and if the BOD of the water sample 7 to be detected is expected to be too high, certain dilution is carried out. If necessary, the method can remove particles and highly toxic substances in the water sample 7 to be detected by adopting modes of precipitation, filtration and the like so as to avoid the situations of channel blockage, yeast inactivation, death and the like.
The utility model discloses an among the micro-fluidic chemiluminescence detection device, because can contain some metal ions (like iron ion, copper ion) usually in the water sample 7 that awaits measuring, these ions can accelerate luminol chemiluminescence reaction, nevertheless can not influence luminous total amount. In the detection process, as long as it is ensured that in the monitored time window (corresponding to the path length of each channel of the chemiluminescence region 5 of the microfluidic chip 3 at a certain flow rate), the active oxygen species generated by the oxidation of the organic substance by the microorganism can sufficiently react with the luminol reaction system solution to emit light, and the measured total amount of light emission can be used for measuring the content of the organic substance. In addition, too high a concentration of metal ions may significantly reduce the metabolic activity of the microorganism, and the adverse effects thereof may be avoided by precipitation filtration or addition of a masking agent.
Further, in order to ensure that the organic substances in the water sample 7 to be tested can be sufficiently oxidized in the biochemical reaction region (including the reaction region and the chemiluminescence region), the concentration of the organic substances in the water sample cannot be too high. When the BOD of the water sample to be measured is predicted to be too high, a certain dilution measure needs to be adopted in advance, and the dilution operation can be regulated and controlled within a certain range on the micro-fluidic chip 3: for example, the sample injection volume ratio of the water sample 7 to be detected and the reaction detection object is controlled by adjusting the flow rate ratio of sample injection of the microfluidic chip 3, so that water sample dilution of different degrees is realized.
It should be noted that theoretical BOD refers to oxygen consumption for complete reaction of organic matter, and the corresponding oxidation process usually requires several tens or hundreds of days, which reflects the absolute content of organic matter in water. Biochemical oxygen demand BOD for five days measured by standard inoculation dilution method5Only an approximate estimate of the theoretical BOD, and correspondingly BOD7，BOD10And BOD20Etc., currently BOD is generally adopted5As a water quality measure. The utility model provides an among the micro-fluidic chemiluminescence detection device, adopt modes such as excessive high activity microorganism, regulation and control reaction temperature, dilution sample to let the organic matter react as fast as possible completely and then the ration turns into luminous signal, the BOD that measuresCLThe theoretical BOD can also be estimated efficiently.
Because the BOD in a certain organic matter concentration interval is generated when the organic matter composition of the water sample 7 to be tested does not change greatlyCLAnd BOD5Has stable quantitative relation and can be determined according to the known BOD5The standard water sample is measured to prepare a standard curve, so that the BOD is measured in real time in the actual sample detectionCLConverted to BOD5And then whether the water body to be detected has abnormal pollution condition or not is determined in real time. Wherein, the standard water sample needs to be obtained from the water sample to be measured to ensure that the composition of the sample organic matter for making the standard curve is as close as possible to the composition in actual measurement.
Specifically, the steps of BOD detection by using the microfluidic chemiluminescence detection device are as follows:
1. the microfluidic control system 1 is formed by assembling and connecting a microfluidic chip 3, three peristaltic pumps, a water sample 7 to be detected, a yeast suspension 8, a luminol reaction system solution 9 and a waste liquid 10 according to a structure shown in figure 1. The micro-fluidic chip 3 is arranged at a specific position in the darkroom, so that the reaction area 4 of the micro-fluidic chip is arranged above a temperature control device, such as a micro temperature control device, the chemiluminescence area 5 is aligned with a chemiluminescence detector, such as a photomultiplier, and the micro-fluidic chip 3 is connected with other external devices through pipelines.
2. According to the known BOD5And (3) carrying out a pre-experiment on the standard water sample of the value to optimize experiment detail parameters such as the concentration of various reaction detection reagent, the flow rate of the fluid and the like, and making a standard curve. And if the BOD value of the water sample to be measured is estimated to be too high, diluting the sample.
3. And simultaneously injecting the water sample to be detected and the yeast suspension into the micro-fluidic chip according to a set flow rate by using a peristaltic pump to initiate a biochemical reaction to form a mixed solution. After the mixed solution passes through the reaction area (which can be estimated by flow rate and time), injecting the mixed solution into a luminol reaction system to further initiate a chemiluminescence reaction.
4. After the liquid is filled in the spiral channel of the chemiluminescence area, the chemiluminescence signal tends to be stable, and then the data can be formally recorded. The secondary flow generated by the fluid in the spiral channel can promote the solution to be quickly mixed and reacted, and then reliable chemiluminescence data is generated.
Utilize above-mentioned according to the utility model discloses a micro-fluidic chemiluminescence detection device has following advantage:
1. the utility model adopts the micro-fluidic chip chemiluminescence detection method to determine BODCLAnd then estimate the corresponding BOD5The reagent consumption can be greatly reduced, the analysis speed is improved, the portable online detection scheme is realized, and the method can be used for real-time monitoring of the BOD of a water sample.
2. The utility model discloses adopt class tesla valve structure among the micro-fluidic chip, utilize fluid self to flow and produce the clash mixing action, need not realize the fluid stirring with the help of factors such as external electricity, magnetism, machinery, saved the cost, simplified the operation.
3. The micro-fluidic chip avoids structures such as grooves and pockets, and can avoid long-term retention of fluid in corners, so that the fluid basically flows in the pipeline according to the injection sequence, and the reliability of online real-time analysis is ensured.
4. By utilizing the property of 'time-space conversion' in the micro-fluidic, under the set pipeline length, the biochemical reaction time can be regulated and controlled only by adjusting the flow rate of the peristaltic pump, the frequent operations of reaction initiation, termination, timing and the like in the conventional test tube method can be avoided, the online analysis is convenient, and the errors caused by manual operation are reduced.
The microfluidic chemiluminescent detection device according to the present invention is described above by way of example with reference to the accompanying drawings. However, it will be appreciated by those skilled in the art that various modifications may be made to the microfluidic chemiluminescent detection device described above without departing from the scope of the present invention. Therefore, the scope of the present invention should be determined by the content of the appended claims.
Claims (8)
1. A microfluidic chemiluminescence detection device is characterized by comprising a chemiluminescence detection system and a microfluidic system arranged in the chemiluminescence detection system; wherein,
the micro-fluidic system comprises a micro-fluidic chip, a water sample to be detected and a reaction detection object, wherein the water sample to be detected is connected with the micro-fluidic chip, and the reaction detection object is connected with the micro-fluidic chip and corresponds to the water sample to be detected;
the water sample to be detected and the reaction detection object are both injected into the micro-fluidic chip, and react in the micro-fluidic chip to generate a light-emitting signal;
the chemiluminescence detection system is used for monitoring the luminescence signal in real time so as to realize online BOD detection of the water sample to be detected.
2. The microfluidic chemiluminescent detection device of claim 1 wherein,
the reaction detector comprises a yeast suspension and a luminol reaction system solution.
3. The microfluidic chemiluminescent detection device of claim 2 wherein the first and second electrodes are electrically connected,
the microfluidic chip comprises a reaction area and a chemiluminescence area which are mutually communicated;
injecting the water sample to be detected and the yeast suspension liquid into the reaction area at the same time, mixing and fully reacting the water sample and the yeast suspension liquid, and inputting the mixture into the chemiluminescence area;
and injecting the luminol reaction system solution into the chemiluminescence area and carrying out chemical reaction with the mixed solution of the water sample to be detected and the yeast suspension to generate the luminescence signal.
4. The microfluidic chemiluminescent detection device of claim 3 wherein,
the reaction zone comprises a pipeline of a Tesla-like valve structure;
the pipeline comprises at least two mixing parts and a bending part for connecting the two adjacent mixing parts;
the mixing part comprises regularly distributed shunting nodes and collision mixing nodes.
5. The microfluidic chemiluminescent detection device of claim 3 wherein,
the chemiluminescence detection system comprises a darkroom, a photomultiplier and a micro temperature control device;
the micro-fluidic chip is placed in the dark room, the photomultiplier is arranged below the chemiluminescence area, and the micro temperature control device is arranged below the reaction area.
6. The microfluidic chemiluminescent detection device of claim 3 wherein,
the chemiluminescent region comprises a helical channel;
and mixing the luminol reaction system solution and the mixed solution in the spiral channel to perform biochemical reaction.
7. The microfluidic chemiluminescent detection device of claim 1 wherein,
the microfluidic chip comprises three inlets and one outlet;
and the reaction detection object and the water sample to be detected are respectively injected into the microfluidic chip from corresponding inlets under the action of a peristaltic pump, and waste liquid after detection is discharged through the outlets.
8. The microfluidic chemiluminescent detection device of claim 1 wherein the reactive detector is injected into the microfluidic chip at a predetermined flow rate by a peristaltic pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021874014.3U CN212780531U (en) | 2020-09-01 | 2020-09-01 | Micro-fluidic chemiluminescence detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021874014.3U CN212780531U (en) | 2020-09-01 | 2020-09-01 | Micro-fluidic chemiluminescence detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212780531U true CN212780531U (en) | 2021-03-23 |
Family
ID=75056087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021874014.3U Active CN212780531U (en) | 2020-09-01 | 2020-09-01 | Micro-fluidic chemiluminescence detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212780531U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2781351C1 (en) * | 2021-12-27 | 2022-10-11 | федеральное государственное автономное образовательное учреждение высшего образования "Национальный исследовательский университет ИТМО" (Университет ИТМО) | Chemiluminescent analysis device |
-
2020
- 2020-09-01 CN CN202021874014.3U patent/CN212780531U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2781351C1 (en) * | 2021-12-27 | 2022-10-11 | федеральное государственное автономное образовательное учреждение высшего образования "Национальный исследовательский университет ИТМО" (Университет ИТМО) | Chemiluminescent analysis device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112067603A (en) | Microfluidic chemiluminescence detection device and method | |
| CN101004386A (en) | Method for measuring chemical oxygen demand and total organic carbon by mode of oxidizing floating injected ozone | |
| US10302552B2 (en) | Apparatus, composition and method for determination of chemical oxidation demand | |
| CN107505369B (en) | Bioelectrochemical system and online biochemical oxygen demand monitoring device and method thereof | |
| CN111707659A (en) | Luminous bacteria-based water quality comprehensive biotoxicity analyzer | |
| CN103528915B (en) | Intelligent pressure-sensitive type biochemical oxygen demand (BOD) measuring instrument | |
| Wang et al. | Computer applications to fermentation processes | |
| Wang et al. | Design, optimization and application of a highly sensitive microbial electrolytic cell-based BOD biosensor | |
| CN103336045B (en) | Device for on-line detection and automatic alarm of toxic substances and detection method of toxic substances | |
| CN212780531U (en) | Micro-fluidic chemiluminescence detection device | |
| US9518922B2 (en) | Arrangement for in situ measurement of at least the oxygen content within a solids heap | |
| CN108426938A (en) | A kind of direct bioelectrode analytical equipment and analysis method for zymotic fluid detection | |
| CN114034687B (en) | Method and device for high-precision detection of calcium ions | |
| CN212568486U (en) | Luminous bacteria-based water quality comprehensive biotoxicity analyzer | |
| Worsfold | The bio-analytical potential of flow injection analysis | |
| CN102692441B (en) | Detection device and method of reduction activity of deposit sediment microorganism | |
| Easty et al. | Continuous electrochemical determination of cyanide. Application to cyanogenic glycosides in Sudan grass | |
| CN211179536U (en) | ATP on-line measuring instrument | |
| CN211122516U (en) | Wide-spectrum five-day biochemical oxygen demand online monitoring device | |
| Yaping et al. | Spectrophotometric determination of urinary iodine by flow-injection analysis with on-line catalytic digestion | |
| JP2595141B2 (en) | Phosphate ion concentration measurement device | |
| CN208224158U (en) | A kind of direct bioelectrode analytical equipment for fermentation liquid detection | |
| JP3074361B2 (en) | Quantitative analyzer | |
| CN218157690U (en) | Device based on dissolved oxygen survey BOD content in aquatic | |
| CN114965446B (en) | Method for measuring chemical oxygen demand in sewage and wastewater by using chemiluminescence quenching method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |