CN105806814B - The automated monitor and its method of multi-cultur es water quality biological toxicity - Google Patents
The automated monitor and its method of multi-cultur es water quality biological toxicity Download PDFInfo
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- CN105806814B CN105806814B CN201610132495.2A CN201610132495A CN105806814B CN 105806814 B CN105806814 B CN 105806814B CN 201610132495 A CN201610132495 A CN 201610132495A CN 105806814 B CN105806814 B CN 105806814B
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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Abstract
The invention discloses a kind of automated monitors of multi-cultur es water quality biological toxicity, the equipment includes PLC control unit, man-machine interaction unit, flow path control unit, recovery storage unit, sample dosing unit, reaction detection unit, the PLC control unit, man-machine interaction unit, flow path control unit, recovery storage unit, sample dosing unit, reaction detection unit is arranged in frame, the PLC control unit and man-machine interaction unit, flow path control unit, reaction detection unit connects, the flow path control unit and recovery storage unit, sample dosing unit, reaction detection unit connects；The invention also discloses a kind of monitoring methods of multi-cultur es water quality biological toxicity, the present invention has many advantages, such as that adaptation multi-cultur es, whole detection, high certainty of measurement, time of measuring is short, bacterium solution dosage is few, small without cross jamming, maintenance, meets the needs of online environment monitoring.
Description
Technical field
The present invention relates to water quality monitorings in environmental monitoring, and in particular to a kind of multi-cultur es water quality biological toxicity
Automated monitor.
Background technology
While along with Chinese society rapid economic development, also getting worse, various pollutants are directly arranged for environmental pollution
It is put into the environment waters such as rivers and lakes, is more than water body self-purification ability, water pollution is constantly aggravated.Traditional water quality monitoring method
It is physico-chemical method, can accurately determines pollutant kind and concentration, but measured one by one to the numerous pollutants of quantity
It needs to consume plenty of time and energy, can not also measure the comprehensive effect of multiple pollutant interaction.
Biological detecting method can make up the deficiency of physico-chemical method, can not only accomplish to cover all referring to mark, can also reflect more
The comprehensive toxicity of kind pollutant interaction, becomes the new direction developed in water quality monitoring field.It is raw used in biological detecting method
Object includes fish, shellfish, water flea, algae, photobacteria etc., and wherein Luminous bacteria is because of rapid reaction, high sensitivity, adaptability
By force, the advantages that repeatability is high, easy to operate and receive significant attention.
Domestic and foreign scholars have done a lot of research and application to photobacteria detection water quality biological toxicity, and have developed test
Instrument.Such as the ToxScreen of BioFix Lumi-10, the CheckLight companies of Bake Mann, MicroLAN companies
The Microtox of iTOXcontrol and TOXmini, SDI company, the Eclox and LUMIStox300 of Hach companies, the country has poly-
The TOX-2000 of light science and technology, power close the LFTOX-2010, the LumiFox 8000 of the bright stone in Shenzhen of science and technology.But this quasi-instrument is being applied
In there is also some problems, can only such as use single culture, fresh water and seawater cannot be adapted to simultaneously and measured；It only reacts and ties in bacterium solution
Fluorescence intensity is measured when beam, cannot monitor the fluorescence intensity change of reaction process；It is low to additionally, there may be the degree of automation, failure rate
Height, time of measuring is long, poor repeatability, there are cross jamming, the problems such as operating cost is high, and manual maintenance amount is big.
Invention content
In view of this, the main purpose of the present invention is to provide a kind of automatic monitorings of multi-cultur es water quality biological toxicity
Equipment.
In order to achieve the above objectives, the technical proposal of the invention is realized in this way：
The embodiment of the present invention provides a kind of automated monitor of multi-cultur es water quality biological toxicity, which includes PLC controls
Unit processed, man-machine interaction unit, flow path control unit, recovery storage unit, sampling dosing unit, reaction detection unit；
The man-machine interaction unit, for interface display and to the recovery storage unit, sampling dosing unit, anti-
The parameter of detection unit is answered to be configured；
The PLC control unit, supplemental characteristic for receiving man-machine interaction unit setting and is transferred to flow path
Control unit, and receive the testing result of reaction detection unit feedback and be output to the man-machine interaction unit and shown
Show；
The flow path control unit, recovery storage unit, the sampling for receiving the PLC control unit transmission are quantitative single
Member, the supplemental characteristic of reaction detection unit and be issued to successively respectively recovery storage unit, sampling dosing unit, reaction detection
Unit；The recovery storage unit, the feedback of sampling dosing unit, reaction detection unit are received simultaneously；
The recovery storage unit, for receiving to flow path control unit and feedback parameter data and realizing answering for bacterium solution
Soviet Union and preservation；
The sampling dosing unit, for being received to flow path control unit with feedback parameter data and for quantitatively injecting
Reagent；
The reaction detection unit, for receiving with feedback parameter data to flow path control unit and being reacted for bacterium solution
And fluoroscopic examination, it is whole during the reaction to detect fluorescence intensity change curve and feed back to the PLC control unit.
The embodiment of the present invention also provides a kind of automated monitor of multi-cultur es water quality biological toxicity, which includes PLC
Control unit, man-machine interaction unit, flow path control unit, recovery storage unit, sampling dosing unit, reaction detection unit, institute
State PLC control unit, man-machine interaction unit, flow path control unit, recovery storage unit, sampling dosing unit, reaction detection list
Member is arranged in frame, and the PLC control unit is connect with man-machine interaction unit, flow path control unit, reaction detection unit,
The flow path control unit is connect with recovery storage unit, sampling dosing unit, reaction detection unit；The man-machine interaction unit
It is arranged at the top of frame, the reaction detection unit and recovery storage unit are arranged at the middle part of frame, and the sampling is quantitative
Unit is arranged at the middle part and lower part of frame.
Preferably, the recovery storage unit is respectively used to control first including the first bacterium solution pond, the second bacterium solution pond, two pieces
The Programmable Temperature control module of temperature in bacterium solution pond, the second bacterium solution pond, first bacterium solution pond, the second bacterium solution pond are separately connected
There are the first mixing pump, the second mixing pump.
Preferably, the sampling dosing unit includes water sample injection unit, bacterium solution injection unit, positive injection unit, institute
It includes multi-channel synchronous peristaltic pump to state water sample injection unit, and the bacterium solution injection unit includes bacterium solution multiposition valve, the positive note
It includes positive reagent multiposition valve to enter unit, and water sample, pure water, salt are drawn in one end of the multi-channel synchronous peristaltic pump by pipeline
Water, the other end are connect with reaction detection unit for conveying water sample, pure water, brine；It is connected with and is used on the bacterium solution multiposition valve
Quantitative selection and inject the measurement bacterium solution quantitative loop of bacterium solution, reference bacterium solution quantitative loop, bacterium solution constant displacement pump, bacterium solution injection pump, with it is anti-
The pipeline for answering detection unit to be connected to, and the first bacterium solution pond of the recovery storage unit, the second bacterium solution pond are through bacterium solution selector valve
It is connect with the bacterium solution multiposition valve；The sun for quantitative selection and injection positive reagent is connected on the positive reagent multiposition valve
Property reagent quantitative ring, positive reagent constant displacement pump, positive reagent injection pump, the pipeline being connected to reaction detection unit.
Preferably, it is provided with water sample selector valve on the pipeline of the water sample, pure water and the connection of multi-channel synchronous peristaltic pump.
Preferably, the reaction detection unit includes reference cell, measuring cell, third mixing pump, the 4th mixing pump, two light
Electric multiplier tube, Programmable Temperature control module；The reference cell, measuring cell lower end are separately connected third mixing pump, the 4th stirring
Pump, the reference cell, measuring cell include injection port, leakage fluid dram, sealing piston, expand O circles, piston rod, driving motor, described
Sealing piston and piston rod are located at the inner wall of measuring cell or reference cell, and the expansion O is enclosed between piston rod and sealing piston,
The piston rod is cylindrical hollow structure, and for connecting sealing piston and driving motor, the photomultiplier setting is measuring
Outside pond or reference cell, for receiving the fluorescent radiation in measuring cell or reference cell；It is provided with outside the measuring cell and reference cell
Programmable Temperature control module for providing stationary temperature environment for measuring cell, reference cell and photomultiplier；It is described to take
The bacterium solution multiposition valve of sample dosing unit is connect with the reference cell, measuring cell respectively, the positive reagent of the sampling dosing unit
Multiposition valve is connect with the measuring cell.
The embodiment of the present invention also provides a kind of monitoring method of multi-cultur es water quality biological toxicity, and this method passes through following steps
It realizes：
Step S1：Prepare before test, prepare required pure water, brine, positive reagent and bacterium solution, is arranged according to strain used
Corresponding recovery preservation condition and reaction detection temperature；
Step S2：Inject water sample and pure water, multi-channel synchronous peristaltic pump extracts water sample, pure water and brine simultaneously, and by water
It is injected into the measuring cell of reaction detection unit after sample and brine mixing, reaction detection unit is injected into after pure water and brine are mixed
Reference cell, the synchronous transhipment simultaneously of four channels of the multi-channel synchronous peristaltic pump, it is ensured that water sample/brine ratio and pure water/
Brine ratio is accurately consistent, and water sample is injected into reaction detection unit simultaneously with pure water；
Step S3：Bacterium solution is injected, bacterium solution multiposition valve rotates to sample position, and bacterium solution constant displacement pump extracts bacterium solution to reference bacterium solution
Quantitative loop and measurement bacterium solution quantitative loop, the bacterium solution multiposition valve rotate to injection phase, and bacterium solution injection pump simultaneously will be in quantitative loop
Bacterium solution be injected into the measuring cell and reference cell, the pipeline of the bacterium solution injection pump is divided into two with three-way interface, ensure two
Bacterium solution in a quantitative loop injects measuring cell and reference cell again simultaneously, the bacterium solution in described two quantitative loops be injected into measuring cell and
After reference cell, bacterium solution extra in the bacterium solution multiposition valve and pipeline is exhausted, ensures do not have in pipeline when next time extracts bacterium solution
Last time remaining bacterium solution；
Step S4：Reaction detection, reaction time are arranged in 1-99min, you can the setting reaction time by hand, also can be by
PLC control unit sets the reaction time automatically according to the variation tendency of fluorescence intensity in reaction process, initial in the reaction time
Stage, the 4th mixing pump and third mixing pump blow to measuring cell and reference cell stir respectively, to ensure that bacterium solution is fully reacted；Two
A photomultiplier is operated in single photon detection pattern, while detecting the fluorescence intensity in measuring cell and reference cell, is noted from bacterium solution
Enter and terminate up to reaction, complete monitoring detection fluorescence intensity is simultaneously sent to PLC control unit, and the PLC control unit is according to just
Beginning fluorescence intensity terminates fluorescence intensity change trend in fluorescence intensity and reaction process, comprehensive judgement water quality biological toxicity；
Step S5：Drain is cleaned, and after reaction detection process, the 4th pond mixing pump and the reversion of third mixing pump pass through
The hollow structure and leakage fluid dram of piston rod empty the measuring cell and reference cell, and driving motor drives piston rod to move up and down,
The piston rod head sealing piston cleaning measuring cell and reference cell inner wall；
Step S6：Testing procedure is S2-S5, water sample selector valve is opened during step S2 so that measuring cell and reference
Chi Jun injects pure water；Negative inhibiting rate is calculated, is determined as qualification less than 5%；
Step S7：Measuring process is S2-S5, water sample selector valve is opened during step S2 so that measuring cell and reference
Chi Jun injects pure water, increases to measuring cell in S3 steps and injects positive reagent；Positive inhibiting rate is calculated, is judged to closing more than 90%
Lattice.
Compared with prior art, beneficial effects of the present invention：
Multi-cultur es, whole detection, high certainty of measurement, time of measuring is short, bacterium solution dosage is few, without intersection with adapting to by the present invention
Interference, the advantages that maintenance is small, meets the needs of online environment monitoring.
Description of the drawings
Fig. 1 provides a kind of composition frame chart of the automated monitor of multi-cultur es water quality biological toxicity for the embodiment of the present invention；
Fig. 2 provides a kind of front view of the automated monitor of multi-cultur es water quality biological toxicity for the embodiment of the present invention
Fig. 3 provides a kind of flow circuit diagram of the automated monitor of multi-cultur es water quality biological toxicity for the embodiment of the present invention
Fig. 4 provides a kind of reaction detection of the automated monitor of multi-cultur es water quality biological toxicity for the embodiment of the present invention
The structure chart of unit；
Fig. 5 is the side view of Fig. 4；
The positions Fig. 6 embodiment of the present invention also provides a kind of the step of monitoring method of multi-cultur es water quality biological toxicity and schemes.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
The embodiment of the present invention provides a kind of automated monitor of multi-cultur es water quality biological toxicity, as shown in Figure 1, the equipment
Including PLC control unit, man-machine interaction unit, flow path control unit, recovery storage unit, sampling dosing unit, reaction detection
Unit；
The man-machine interaction unit, for interface display and to the recovery storage unit, sampling dosing unit, anti-
The parameter of detection unit is answered to be configured；
The PLC control unit, supplemental characteristic for receiving man-machine interaction unit setting and is transferred to flow path
Control unit, and receive the testing result of reaction detection unit feedback and be output to the man-machine interaction unit and shown
Show；
The flow path control unit, recovery storage unit, the sampling for receiving the PLC control unit transmission are quantitative single
Member, the supplemental characteristic of reaction detection unit and be issued to successively respectively recovery storage unit, sampling dosing unit, reaction detection
Unit；The recovery storage unit, the feedback of sampling dosing unit, reaction detection unit are received simultaneously；
The recovery storage unit, for receiving to flow path control unit and feedback parameter data and realizing answering for bacterium solution
Soviet Union and preservation；
Specifically, the recovery storage unit is used for the recovery and preservation of bacterium solution, has Programmable Temperature control function, can
The requirement for meeting different strain recovery and preserving；
The sampling dosing unit, for being received to flow path control unit with feedback parameter data and for quantitatively injecting
Reagent；
Specifically, the sampling dosing unit reaches 0.5 μ L, has for quantitatively reagents, the quantitative accuracy such as injection bacterium solution
Simultaneous implantation function, deviation caused by solution reagent injection length is inconsistent.
The reaction detection unit, for receiving with feedback parameter data to flow path control unit and being reacted for bacterium solution
And fluoroscopic examination, it is whole during the reaction to detect fluorescence intensity change curve and feed back to the PLC control unit.
Specifically, the reaction detection unit integrates bacterium solution reaction and fluoroscopic examination, has Programmable Temperature control
Function, and whole detection fluorescence intensity change curve during the reaction.
The embodiment of the present invention also provides a kind of automated monitor of multi-cultur es water quality biological toxicity, such as the institute of Fig. 2,3,4,5
Show, which includes PLC control unit, man-machine interaction unit 1, flow path control unit, recovery storage unit 5, the quantitative list of sampling
Member, reaction detection unit 9, the PLC control unit, flow path control unit, recovery storage unit 5, take man-machine interaction unit 1
Sample dosing unit, reaction detection unit 9 are arranged in frame, the PLC control unit and man-machine interaction unit 1, flow path control
Unit processed, reaction detection unit 9 connect, and the flow path control unit and recovery storage unit 5 sample dosing unit, react inspection
Unit 9 is surveyed to connect；The man-machine interaction unit 1 is arranged in the top of frame, the reaction detection unit 9 and recovery storage unit
5 are arranged at the middle part of frame, and the sampling dosing unit is arranged at the middle part and lower part of frame.
As shown in figure 3, the recovery storage unit 5 is respectively used to including the first bacterium solution pond 26,27, two pieces of the second bacterium solution pond
Control the first bacterium solution pond 26, in the second bacterium solution pond 27 temperature Programmable Temperature control module 35, first bacterium solution pond 26,
Two bacterium solution ponds 27 are connected separately with the first mixing pump 24, the second mixing pump 25.
First mixing pump 24, the second mixing pump 25 are piezoelectric pump, for carrying out air blowing stirring to bacterium solution, extend bacterium solution
Service life.
The Programmable Temperature control module 35 plays bacterium solution in the work of recovery and preservation for controlling bacterium solution pond temperature
With heating refrigeration part is semiconductor chilling plate, and control mode is PID control, and the way of output exports for PwM, can be in (0-
DEG C 45) temperature curve is arbitrarily set in, temperature accuracy reaches 0.1 DEG C, meets the recovery of different bacterium solutions and preserves and requires.Research is not
Same strain (such as Fermi operator, bright bacillus, Qinghai Vibrion) is recovered and preserves required optimum temperature environment, and setting is recovered
The temperature curve parameter of storage unit, including recovery temperature value, recovery temperature rate-of-rise, recovery temperature fall off rate, preservation
Temperature curve etc., to adapt to different strain requirement.
As shown in figure 3, the sampling dosing unit includes water sample injection unit, bacterium solution injection unit, positive injection unit,
The water sample injection unit includes multi-channel synchronous peristaltic pump 3, and the bacterium solution injection unit includes bacterium solution multiposition valve 4, the sun
Property injection unit include positive reagent multiposition valve 8, one end of the multi-channel synchronous peristaltic pump 3 by pipeline draw water sample 13,
Pure water 11, brine 6, the other end are connect with reaction detection unit 9 for conveying water sample 13, pure water 11, brine 6；The bacterium solution is more
It is fixed for measurement bacterium solution quantitative loop 20, reference bacterium solution quantitative loop 21, the bacterium solution of quantitative selection and injection bacterium solution to be connected on the valve 4 of position
Amount pump 2, bacterium solution injection pump 22, the pipeline being connected to reaction detection unit 9, and the first bacterium solution of the recovery storage unit 5
Pond 26, the second bacterium solution pond 27 are connect through bacterium solution selector valve with the bacterium solution multiposition valve 4；It is connected on the positive reagent multiposition valve 8
It is useful for quantitative selection and injects the positive reagent quantitative loop 18 of positive reagent 10, positive reagent constant displacement pump 7, positive reagent injection
Pump 19, the pipeline being connected to reaction detection unit 9.
It is provided with water sample selector valve 12 on the pipeline that the water sample 13, pure water 11 are connect with multi-channel synchronous peristaltic pump 3.
Specifically, the water sample injection unit includes multi-channel synchronous peristaltic pump 3 and water sample selector valve 12, is used for water sample
13, the mixing and injection of pure water 11 and brine 6.In water sample detection, pure water 11 is injected to the reference cell 14 of reaction detection unit 9
With the mixed liquor of brine 6, the mixed liquor of water sample 13 and brine 6 is injected to the measuring cell 15 of reaction detection unit 9.It is verified in feminine gender
When with positive verification, the mixed liquor of pure water 11 and brine 6 is injected to reference cell 14 and measuring cell 15.The multi-channel synchronous is compacted
Dynamic pump 3 synchronizes peristaltic pump for four-way, and entrance is separately connected water sample 13, pure water 11 and brine 6, exports water sample 13 and salt
Water 6 is injected into measuring cell 15 after mixing, and reference cell 14 is injected into after pure water 11 and brine 6 are mixed.Multi-channel synchronous peristaltic pump 3
The synchronous transhipment simultaneously of each channel, it can be achieved that water sample/brine ratio and pure water/brine ratio are accurately consistent, and ensure water sample 13
It is injected into reaction detection unit 9 simultaneously with pure water 11.The water sample selector valve 12 is for switching water sample 13 and pure water 11, in feminine gender
When verification and positive verification, the injection pure water 11 into measuring cell 15 is realized.
The bacterium solution injection unit includes bacterium solution multiposition valve 4, reference bacterium solution quantitative loop 21, measures bacterium solution quantitative loop 20, bacterium
Liquid constant displacement pump 2, bacterium solution injection pump 22, bacterium solution selector valve 23.The bacterium solution multiposition valve 4 is ten valves, valve body and spool using resistance to
Corrosive Materia (such as stainless steel, PAKE, PEEK, PPS, PTEF).The reference bacterium solution quantitative loop 21 and measurement bacterium solution are quantitative
Ring 20 is the PTFE tube of regular length, and the length of pipe can be adjusted according to bacterium solution dosage.The bacterium solution constant displacement pump 2 is wriggled for single channel
Pump, for providing the power for extracting bacterium solution.The bacterium solution injection pump 22 is piezoelectric pump, for the bacterium solution in quantitative loop to be injected into
Reaction detection unit 9, bacterium solution injection pump 22 divide for two-way, the reaction that can be simultaneously injected separately into the bacterium solution in two quantitative loops
The measuring cell 15 and reference cell 14 of detection unit 9.Bacterium solution only 10 μ L needed for measuring every time, cost only 3-4 members.
It is described the positive injection unit include positive reagent multiposition valve 8, positive reagent quantitative loop 18, positive reagent constant displacement pump 7,
Positive reagent injection pump 19.The positive reagent multiposition valve 8 is six valves, and valve body and spool use corrosion resistant material (if not
Become rusty steel, PAKE, PEEK, PPS, PTEF etc.).The positive reagent quantitative loop 18 is the PTFE tube of regular length, can be according to the positive
Reagent dosage adjusts the length of pipe.The positive reagent constant displacement pump 7 is single channel peristaltic pump, and positive reagent is extracted for providing
Power.The positive reagent injection pump 19 is piezoelectric pump, for the positive reagent in quantitative loop to be injected into reaction detection unit 9
Measuring cell 15.
As shown in Figure 4,5, the reaction detection unit 9 includes reference cell 14, measuring cell 15, third mixing pump the 16, the 4th
Mixing pump 17, two photomultipliers 29, Programmable Temperature control modules 35；The reference cell 14,15 lower end of measuring cell difference
Connect third mixing pump 16, the 4th mixing pump 17, the reference cell 14, measuring cell 15 include injection port 28, leakage fluid dram 33, close
Seal piston 31, expansion O circles 30, piston rod 32, driving motor 34, the sealing piston 31 and piston rod 32 be located at measuring cell 15 or
The inner wall of reference cell 14, for the expansion O circles 30 between piston rod 32 and sealing piston 31, the piston rod 32 is in cylinder
Hollow structure, for connecting sealing piston 31 and driving motor 34, the photomultiplier 29 is arranged in measuring cell 15 or reference cell
Outside 14, for receiving the fluorescent radiation in measuring cell 15 or reference cell 14；It is provided with outside the measuring cell 15 and reference cell 14
Programmable Temperature control module 35 for providing stationary temperature environment for measuring cell, reference cell and photomultiplier；It is described
The bacterium solution multiposition valve 4 of sampling dosing unit is connect with the reference cell 14, measuring cell 15 respectively, the sun of the sampling dosing unit
Property reagent multiposition valve 8 is connect with the measuring cell 15.
Specifically, the third mixing pump 16, the 4th mixing pump 17 are piezoelectric pump, you can for dividing reference cell 14 and measuring
Pond 15, which is blown, stirs, and ensures the abundant reaction of bacterium solution, it can also be used to drain.
The Programmable Temperature control module 35, heating refrigeration part are semiconductor chilling plate, and control mode is controlled for PID
System, the way of output export for PWM, corresponding temperature value can be arranged according to different strain, be measuring cell, reference cell and photomultiplier transit
Pipe provides stationary temperature environment, and temperature accuracy reaches 0.1 DEG C.
The sealing piston 31 is PTFE material, is located at measuring cell 15 (or reference cell 14) inner wall, is used for plugging meter pond
15 (or reference cells 14) and cleaning measuring cell 15 (or reference cell 14).
It is fluorubber, nitrile rubber or silicon rubber that the expansion O, which encloses 30 materials, position piston rod 32 and sealing piston 31 it
Between.Because 31 material of sealing piston be PTFE, measuring cell 15 (or reference cell 14) material be glass, the two coefficient of thermal expansion, therefore
When variation of ambient temperature is larger, there are the phenomenon of leakage between sealing piston 31 and measuring cell 15 (or reference cell 14), utilization is swollen
Swollen O encloses 30 shrinkages of itself, it is ensured that sealing piston 31 and measuring cell 15 (or reference cell 14) tight fit at a temperature of not.
The piston rod 32 is stainless steel material, is cylindrical hollow structure, and hollow space is used for drain, piston rod connection
Sealing piston 31 and driving motor 34.It drives sealing piston 31 to move up and down under the action of driving motor 34, reaches cleaning and survey
Measure the effect in pond 15 and 14 inner wall of reference cell.
The photomultiplier 29 is operated in single photon detection pattern, and photomultiplier 29 receives (or the ginseng of measuring cell 15
Than pond 14) in fluorescent radiation, set enhanced processing before menstruation, level screens removal noise, and fluorescence intensity signals are converted into arteries and veins
Signal is rushed, pulse signal is converted to Transistor-Transistor Logic level by voltage comparator, is counted to TTL using FPGA and is sent to PLC controls system
System.Photomultiplier 29 is when bacterium solution is injected into measuring cell 15 and reference cell 14, until reaction terminates whole detection fluorescence intensity.
The bacterium solution reaction time can be arranged in (1-99) min, you can the setting reaction time by hand, also can be by PLC control system according to anti-
The variation tendency of fluorescence intensity sets the reaction time automatically during answering.
The flow path control unit is by designated program driving bacterium solution multiposition valve 4, positive reagent multiposition valve 8, multi-channel synchronous
Peristaltic pump 3, bacterium solution constant displacement pump 2, positive reagent constant displacement pump 7, positive reagent injection pump 19, bacterium solution injection pump 22, third mixing pump
16, the flow paths device such as the 4th mixing pump 17, the first mixing pump 25, the second mixing pump 26, Programmable Temperature control module 35.
The embodiment of the present invention also provides a kind of monitoring method of multi-cultur es water quality biological toxicity, as shown in fig. 6, this method is logical
Cross following steps realization：
Step S1：Prepare before test, required pure water 11, brine 6, positive reagent 10 and bacterium solution is prepared, according to bacteria used thereby
Corresponding recovery preservation condition and reaction detection temperature is arranged in kind；
Step S2：Injection water sample and pure water, multi-channel synchronous peristaltic pump 3 extract water sample 13, pure water 11 and brine 6 simultaneously,
And the measuring cell 15 of reaction detection unit 9 is injected into after mixing water sample 13 and brine 6, it is noted after pure water 11 and brine 6 are mixed
Enter the reference cell 14 to reaction detection unit 9, four channels synchronous transhipment simultaneously of the multi-channel synchronous peristaltic pump 3 can be protected
It demonstrate,proves water sample/brine ratio and pure water/brine ratio is accurately consistent, and water sample is injected into reaction detection unit 9 simultaneously with pure water；
Step S3：Bacterium solution is injected, bacterium solution multiposition valve 4 rotates to sample position, and bacterium solution constant displacement pump 2 extracts bacterium solution to reference bacterium
Liquid quantitative loop 21 and measurement bacterium solution quantitative loop 20, the bacterium solution multiposition valve 4 rotate to injection phase, and bacterium solution injection pump 22 simultaneously will
Bacterium solution in quantitative loop is injected into the measuring cell 15 and reference cell 14, the pipeline three-way interface one of the bacterium solution injection pump 22
It is divided into two, ensures the bacterium solution in two quantitative loops while injects measuring cell 15 and reference cell 14 again, in described two quantitative loop
After bacterium solution is injected into measuring cell 15 and reference cell 14, bacterium solution extra in the bacterium solution multiposition valve 4 and pipeline is exhausted, under guarantee
Without last time remaining bacterium solution in pipeline when secondary extraction bacterium solution；
Step S4：Reaction detection, reaction time are arranged in 1-99min, you can the setting reaction time by hand, also can be by
PLC control unit sets the reaction time automatically according to the variation tendency of fluorescence intensity in reaction process, initial in the reaction time
Stage, the 4th mixing pump 17 and third mixing pump 16 blow to measuring cell 15 and reference cell 14 stir respectively, to ensure that bacterium solution is filled
Divide reaction；Two photomultipliers 29 are operated in single photon detection pattern, while detecting glimmering in measuring cell 15 and reference cell 14
Luminous intensity is injected from bacterium solution and is terminated up to reaction, and complete monitoring detection fluorescence intensity is simultaneously sent to PLC control unit, the PLC
Control unit is according to fluorescence intensity change trend, comprehensive judgement water in initial fluorescent intensity, end fluorescence intensity and reaction process
Matter bio-toxicity；
Step S5：Drain is cleaned, and after reaction detection process, the 4th pond mixing pump 17 and third mixing pump 16 invert,
By the hollow structure and leakage fluid dram of piston rod 32, the measuring cell 15 and reference cell 14 are emptied, driving motor 34, which drives, lives
Stopper rod 32 moves up and down, and 32 head seal piston 31 of the piston rod cleans measuring cell 15 and 14 inner wall of reference cell；
Step S6：Testing procedure is S2-S5, water sample selector valve 12 is opened during step S2 so that 15 He of measuring cell
Reference cell 14 injects pure water 11；Negative inhibiting rate is calculated, is determined as qualification less than 5%；
Step S7：Measuring process is S2-S5, water sample selector valve 12 is opened during step S2 so that 15 He of measuring cell
Reference cell 14 injects pure water, increases to measuring cell 15 in S3 steps and injects positive reagent 9；Positive inhibiting rate is calculated, is more than
90% is determined as qualification.
As shown in fig. 6, the present invention is divided into water sample detection, negative verification and positive verification；Water sample detection is for measuring water sample
Bio-toxicity；Feminine gender verification, for judging bacterium solution activity, avoids reporting by mistake for negative verification；Positive verification, for judging bacterium
Liquid sensitivity avoids failing to report.Feminine gender verification and positive verification generally carry out primary daily.
The foregoing is only a preferred embodiment of the present invention, is not intended to limit the scope of the present invention.
Claims (6)
1. a kind of automated monitor of multi-cultur es water quality biological toxicity, which is characterized in that the equipment include PLC control unit,
Man-machine interaction unit, flow path control unit, recovery storage unit, sampling dosing unit, reaction detection unit；
The man-machine interaction unit is examined for interface display and to the recovery storage unit, sampling dosing unit, reaction
The parameter for surveying unit is configured；
The PLC control unit, supplemental characteristic for receiving man-machine interaction unit setting and is transferred to flow path control
Unit, and receive the testing result of reaction detection unit feedback and be output to the man-machine interaction unit and shown；
The flow path control unit, for receive the recovery storage unit of PLC control unit transmission, sampling dosing unit,
The supplemental characteristic of reaction detection unit and be issued to successively respectively recovery storage unit, sampling dosing unit, reaction detection list
Member；The recovery storage unit, the feedback of sampling dosing unit, reaction detection unit are received simultaneously；
The recovery storage unit can carry out Programmable Temperature control, for being received and feedback parameter number to flow path control unit
According to and realize the recovery and preservation of bacterium solution；
The sampling dosing unit can synchronize injection and quantitative accuracy is 0.5 μ L, for being received to flow path control unit
With feedback parameter data and for quantitatively injecting reagent；
The reaction detection unit integrate bacterium solution reaction and fluoroscopic examination, Programmable Temperature control can be carried out, be used for
Flow path control unit is received with feedback parameter data and for bacterium solution reaction and fluoroscopic examination, during the reaction whole detection
Fluorescence intensity change curve and feed back to the PLC control unit.
2. a kind of automated monitor of multi-cultur es water quality biological toxicity, which is characterized in that the equipment include PLC control unit,
Man-machine interaction unit, flow path control unit, recovery storage unit, sampling dosing unit, reaction detection unit, the PLC controls
Unit, man-machine interaction unit, flow path control unit, recovery storage unit, sampling dosing unit, reaction detection unit are arranged at
In frame, the PLC control unit is connect with man-machine interaction unit, flow path control unit, reaction detection unit, the flow path control
Unit processed is connect with recovery storage unit, sampling dosing unit, reaction detection unit；The man-machine interaction unit is arranged in frame
Top, the reaction detection unit and recovery storage unit be arranged at the middle part of frame, and the sampling dosing unit setting exists
The middle part and lower part of frame；The sampling dosing unit includes water sample injection unit, bacterium solution injection unit, positive injection unit,
The water sample injection unit includes multi-channel synchronous peristaltic pump, and the bacterium solution injection unit includes bacterium solution multiposition valve, the positive
Injection unit includes positive reagent multiposition valve, and water sample, pure water, salt are drawn in one end of the multi-channel synchronous peristaltic pump by pipeline
Water, the other end are connect with reaction detection unit for conveying water sample, pure water, brine；It is connected with and is used on the bacterium solution multiposition valve
Quantitative selection and inject the measurement bacterium solution quantitative loop of bacterium solution, reference bacterium solution quantitative loop, bacterium solution constant displacement pump, bacterium solution injection pump, with it is anti-
The pipeline for answering detection unit to be connected to, and the first bacterium solution pond of the recovery storage unit, the second bacterium solution pond are through bacterium solution selector valve
It is connect with the bacterium solution multiposition valve；The sun for quantitative selection and injection positive reagent is connected on the positive reagent multiposition valve
Property reagent quantitative ring, positive reagent constant displacement pump, positive reagent injection pump, the pipeline being connected to reaction detection unit.
3. the automated monitor of multi-cultur es water quality biological toxicity according to claim 2, it is characterised in that：The recovery
Storage unit include the first bacterium solution pond, the second bacterium solution pond, two pieces be respectively used to the first bacterium solution pond of control, temperature in the second bacterium solution pond
Programmable Temperature control module, first bacterium solution pond, the second bacterium solution pond be connected separately with the first mixing pump, second stirring
Pump.
4. the automated monitor of multi-cultur es water quality biological toxicity according to claim 2, it is characterised in that：The water
It is provided with water sample selector valve on the pipeline of sample, pure water and the connection of multi-channel synchronous peristaltic pump.
5. the automated monitor of multi-cultur es water quality biological toxicity according to claim 4, it is characterised in that：The reaction
Detection unit includes reference cell, measuring cell, third mixing pump, the 4th mixing pump, two photomultipliers, Programmable Temperature control
Module；The reference cell, measuring cell lower end are separately connected third mixing pump, the 4th mixing pump, and the reference cell, measuring cell wrap
Injection port, leakage fluid dram, sealing piston, expansion O circles, piston rod, driving motor are included, the sealing piston and piston rod, which are located at, to be measured
The inner wall of pond or reference cell, for the expansion O circles between piston rod and sealing piston, the piston rod is cylindrical hollow knot
Structure, for connecting sealing piston and driving motor, the photomultiplier is arranged outside measuring cell or reference cell, for receiving
Fluorescent radiation in measuring cell or reference cell；It is provided with outside the measuring cell and reference cell for being measuring cell, reference cell and light
Electric multiplier tube provides the Programmable Temperature control module of stationary temperature environment；The bacterium solution multiposition valve point of the sampling dosing unit
It is not connect with the reference cell, measuring cell, the positive reagent multiposition valve of the sampling dosing unit is connect with the measuring cell.
6. a kind of monitoring method of multi-cultur es water quality biological toxicity, which is characterized in that this method is realized by following steps：
Step S1：Prepare before test, prepare required pure water, brine, positive reagent and bacterium solution, is arranged according to strain used and is corresponded to
Recovery preservation condition and reaction detection temperature；
Step S2：Inject water sample and pure water, multi-channel synchronous peristaltic pump extracts water sample, pure water and brine simultaneously, and by water sample with
It is injected into the measuring cell of reaction detection unit after brine mixing, reaction detection unit is injected into after pure water and brine 6 are mixed
Reference cell, four channels synchronous transhipment simultaneously of the multi-channel synchronous peristaltic pump, it is ensured that water sample/brine ratio and pure water/salt
Water is more consistent than accurate, and water sample is injected into reaction detection unit simultaneously with pure water；
Step S3：Bacterium solution is injected, bacterium solution multiposition valve rotates to sample position, and it is quantitative to reference bacterium solution that bacterium solution constant displacement pump extracts bacterium solution
Ring and measurement bacterium solution quantitative loop, the bacterium solution multiposition valve rotate to injection phase, and bacterium solution injection pump is simultaneously by the bacterium in quantitative loop
Liquid is injected into the measuring cell and reference cell, and the pipeline of the bacterium solution injection pump is divided into two with three-way interface, ensures that two are determined
It measures the bacterium solution in ring and injects measuring cell and reference cell again simultaneously, the bacterium solution in described two quantitative loops is injected into measuring cell and reference
Chi Hou exhausts bacterium solution extra in the bacterium solution multiposition valve and pipeline, ensure next time extract bacterium solution when pipeline in without last time
Remaining bacterium solution；
Step S4：Reaction detection, reaction time are arranged in 1-99min, you can by hand the setting reaction time, can also be controlled by PLC
Unit processed sets the reaction time automatically according to the variation tendency of fluorescence intensity in reaction process, in the starting stage in reaction time,
4th mixing pump and third mixing pump are blown to measuring cell and reference cell respectively to be stirred, to ensure that bacterium solution is fully reacted；Two light
Electric multiplier tube is operated in single photon detection pattern, while detecting the fluorescence intensity in measuring cell and reference cell, when being injected from bacterium solution
Terminate to reaction, complete monitoring detection fluorescence intensity is simultaneously sent to PLC control unit, and the PLC control unit is according to initial glimmering
Luminous intensity terminates fluorescence intensity change trend in fluorescence intensity and reaction process, comprehensive judgement water quality biological toxicity；
Step S5：Drain is cleaned, and after reaction detection process, the 4th pond mixing pump and the reversion of third mixing pump pass through piston
The hollow structure and leakage fluid dram of bar empty the measuring cell and reference cell, and driving motor drives piston rod to move up and down, described
Piston rod head sealing piston cleans measuring cell and reference cell inner wall；
Step S6：Testing procedure is S2-S5, water sample selector valve is opened during step S2 so that measuring cell and reference cell are equal
Inject pure water；Negative inhibiting rate is calculated, is determined as qualification less than 5%；
Step S7：Measuring process is S2-S5, water sample selector valve is opened during step S2 so that measuring cell and reference cell are equal
Pure water is injected, increases to measuring cell in S3 steps and injects positive examination；Positive inhibiting rate is calculated, is determined as qualification more than 90%.
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| CN106404506A (en) * | 2016-08-25 | 2017-02-15 | 深圳市有为环境科技有限公司 | Residual chlorine treating unit, and water quality biotoxicity detector |
| CN106645611A (en) * | 2016-10-14 | 2017-05-10 | 武汉联宇技术股份有限公司 | Fish and photogenic bacterium-based comprehensive toxicity online analyzer |
| CN107632001B (en) * | 2017-08-08 | 2019-12-31 | 西安电子科技大学 | Sea surface oil spill monitoring method based on non-uniform pulse modulation |
| CN108279230B (en) * | 2018-02-02 | 2020-06-09 | 哈尔滨工业大学 | Micro-flow control type water quality toxicity analysis and detection device and detection and analysis method thereof |
| CN112098617A (en) * | 2020-09-16 | 2020-12-18 | 杭州绿洁环境科技股份有限公司 | Water quality biological comprehensive toxicity monitoring device and water quality biological comprehensive toxicity monitoring method |
| CN113917097A (en) * | 2021-09-27 | 2022-01-11 | 聚光科技(杭州)股份有限公司 | Method for detecting comprehensive toxicity of water sample |
| CN116751667A (en) * | 2023-08-23 | 2023-09-15 | 上海科泽智慧环境科技有限公司 | Multi-strain switchable monitoring and analyzing device and method |
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