CN105806814A - Automatic monitoring equipment and monitoring method for multi-strain water biotoxicity - Google Patents
Automatic monitoring equipment and monitoring method for multi-strain water biotoxicity Download PDFInfo
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Abstract
The invention discloses automatic monitoring equipment for multi-strain water biotoxicity. The automatic monitoring equipment comprises a PCL unit, a man-machine interaction unit, a flow path control unit, an anabiosis preservation unit, a sampling quantifying unit and a reaction detection unit, wherein the PCL unit, the man-machine interaction unit, the flow path control unit, the anabiosis preservation unit, the sampling quantifying unit and the reaction detection unit are all arranged inside a frame; the PCL unit is connected with the man-machine interaction unit, the flow path control unit and the reaction detection unit; the flow path control unit is connected with the anabiosis preservation unit, the sampling quantifying unit and the reaction detection unit. The invention further discloses a monitoring method for the multi-strain water biotoxicity. The automatic monitoring equipment has the advantages of multi-strain applicability, whole-journey detection, high measurement precision, short measurement time, small bacterial liquid amount, no cross interference, small maintenance amount and the like, and requirements of on-line environment monitoring can be met.
Description
Technical field
The present invention relates to water quality monitoring in environmental monitoring field, be specifically related to the automated monitor of a kind of multi-cultur es water quality biological toxicity.
Background technology
While developing rapidly along with Chinese society economy, environmental pollution is also day by day serious, and various pollutant are directly discharged in the environment waters such as rivers and lakes, exceed water body self-purification ability, and water pollution is constantly aggravated.Traditional water quality monitoring method is physico-chemical method, it is possible to determines pollutant kind and concentration accurately, but carries out the pollutant that quantity is various measuring one by one needing consumption plenty of time and energy, also cannot measure the comprehensive effect that multiple pollutant interacts.
Biological detecting method can make up the deficiency of physico-chemical method, can not only accomplish to cover all referring to mark, it is also possible to the comprehensive toxicity that reflection multiple pollutant interacts, become the new direction in the development of water quality monitoring field.Biology used by biological detecting method includes Fish, shellfish, water flea, algae, photobacteria etc., and wherein Luminous bacteria receives significant attention because reacting the advantages such as quick, highly sensitive, strong adaptability, repeatability is high, easy and simple to handle.
Photobacteria is detected water quality biological toxicity and has done a lot of research and application by Chinese scholars, and have developed test instrunment.BioFixLumi-10 such as Beckman company, the ToxScreen of CheckLight company, iTOXcontrol and the TOXmini of MicroLAN company, the Microtox of SDI company, Eclox and the LUMIStox300 of Hach company, the domestic TOX-2000 having optically focused science and technology, power closes the LFTOX-2010, the LumiFox8000 of the bright stone in Shenzhen of science and technology.But this quasi-instrument there is also some problems in the application, as single culture can only be used, it is impossible to simultaneous adaptation fresh water and sea water are measured；Only measure fluorescence intensity when bacterium solution reaction terminates, it is impossible to the fluorescence intensity change of monitoring course of reaction；Additionally, there may be automaticity low, fault rate is high, and the time of measuring is long, and poor repeatability exists cross interference, and operating cost is high, the problems such as manual maintenance amount is big.
Summary of the invention
In view of this, present invention is primarily targeted at the automated monitor that a kind of multi-cultur es water quality biological toxicity is provided.
For reaching above-mentioned purpose, the technical scheme is that and be achieved in that:
The embodiment of the present invention provides the automated monitor of a kind of multi-cultur es water quality biological toxicity, and this equipment includes PCL control unit, man-machine interaction unit, stream control unit, recovery storage unit, sampling dosing unit, reaction detection unit；
Described man-machine interaction unit, for interface display and to described recovery storage unit, sampling dosing unit, reaction detection unit parameter be configured；
Described PCL control unit, for receiving the supplemental characteristic of described man-machine interaction unit setting and being transferred to stream control unit, and the testing result of reception reaction detection unit feedback and output display to described man-machine interaction unit；
Described stream control unit, for receive described PCL control unit transmission recovery storage unit, sampling dosing unit, reaction detection unit supplemental characteristic and be issued to successively respectively recovery storage unit, sampling dosing unit, reaction detection unit；Receive the feedback of described recovery storage unit, sampling dosing unit, reaction detection unit simultaneously；
Described recovery storage unit, for receiving and feedback parameter data and the recovery and the preservation that realize bacterium solution to stream control unit；
Described sampling dosing unit, for receiving to stream control unit and feedback parameter data and for quantitatively injecting reagent；
Described reaction detection unit, for receiving to stream control unit and feedback parameter data and for bacterium solution reaction and fluoroscopic examination, omnidistance detection fluorescence intensity change curve and feed back to described PCL control unit in course of reaction.
The embodiment of the present invention also provides for the automated monitor of a kind of multi-cultur es water quality biological toxicity, this equipment includes PCL control unit, man-machine interaction unit, stream control unit, recovery storage unit, sampling dosing unit, reaction detection unit, described PCL control unit, man-machine interaction unit, stream control unit, recovery storage unit, sampling dosing unit, reaction detection unit is arranged in framework, described PCL control unit and man-machine interaction unit, stream control unit, reaction detection unit connects, described stream control unit and recovery storage unit, sampling dosing unit, reaction detection unit connects；Described man-machine interaction unit is arranged on the top of framework, described reaction detection unit and recovery storage unit, is arranged on the middle part of framework, and described sampling dosing unit is arranged on middle part and the bottom of framework.
Preferably, described recovery storage unit include the first bacterium solution pond, the second bacterium solution pond, two pieces be respectively used to control the Programmable Temperature of temperature in the first bacterium solution pond, the second bacterium solution pond and control module, described first bacterium solution pond, the second bacterium solution pond are connected to the first mixing pump, the second mixing pump.
Preferably, described sampling dosing unit includes water sample injection unit, bacterium solution injection unit, positive injection unit, described water sample injection unit includes multi-channel synchronous peristaltic pump, described bacterium solution injection unit includes bacterium solution multiposition valve, described positive injection unit includes positive reagent multiposition valve, water sample, pure water, saline are drawn by pipeline in one end of described multi-channel synchronous peristaltic pump, and the other end is connected with reaction detection unit and is used for carrying water sample, pure water, saline；Described bacterium solution multiposition valve is connected to for quantitative selection and the pipeline injecting the measurement bacterium solution quantitative loop of bacterium solution, reference bacterium solution quantitative loop, bacterium solution dosing pump, bacterium solution injection pump connect with reaction detection unit, and the first bacterium solution pond of described recovery storage unit, the second bacterium solution pond are connected with described bacterium solution multiposition valve through bacterium solution selector valve；Described positive reagent multiposition valve is connected to the pipeline connected with reaction detection unit with the injection positive reagent quantitative loop of positive reagent, positive reagent dosing pump, positive reagent injection pump for quantitative selection.
Preferably, the pipeline that described water sample, pure water are connected with multi-channel synchronous peristaltic pump is provided with water sample selector valve.
Preferably, described reaction detection unit includes reference cell, measuring cell, the 3rd mixing pump, the 4th mixing pump, two photomultiplier tubes, Programmable Temperature control modules；Described reference cell, measuring cell lower end connect the 3rd mixing pump, the 4th mixing pump respectively, described reference cell, measuring cell all include injection port, leakage fluid dram, sealing piston, expand O-ring, piston rod, drive motor, described sealing piston and piston rod are positioned at the inwall of measuring cell or reference cell, described expansion O-ring is at piston rod and seals between piston, described piston rod is cylindrical hollow structure, for connecting sealing piston and drive motor, described photomultiplier tube is arranged on outside measuring cell or reference cell, for receiving the fluorescent radiation in measuring cell or reference cell；Described measuring cell and reference cell are outside equipped with for providing the Programmable Temperature of stationary temperature environment to control module for measuring cell, reference cell and photomultiplier tube；The bacterium solution multiposition valve of described sampling dosing unit is connected with described reference cell, measuring cell respectively, and the positive reagent multiposition valve of described sampling dosing unit is connected with described measuring cell.
The embodiment of the present invention also provides for the monitoring method of a kind of multi-cultur es water quality biological toxicity, and the method is realized by following steps:
Step S1: prepare before test, the required pure water of preparation, saline, positive reagent and bacterium solution, recovery preservation condition and the reaction detection temperature of correspondence are set according to strain used；
Step S2: inject water sample and pure water, multi-channel synchronous peristaltic pump extracts water sample, pure water and saline simultaneously, and after water sample and saline being mixed, it is injected into the measuring cell of reaction detection unit, the reference cell of reaction detection unit it is injected into after pure water and saline being mixed, four passages of described multi-channel synchronous peristaltic pump synchronize transhipment simultaneously, can ensure that water sample/brine ratio is accurately consistent with pure water/brine ratio, and water sample and pure water are simultaneously injected into reaction detection unit；
Step S3: inject bacterium solution, bacterium solution multiposition valve rotates to sample position, bacterium solution dosing pump extracts bacterium solution and to reference bacterium solution quantitative loop and measures bacterium solution quantitative loop, described bacterium solution multiposition valve rotates to injection phase, bacterium solution in quantitative loop is injected into described measuring cell and reference cell by bacterium solution injection pump simultaneously, the pipeline three-way interface of described bacterium solution injection pump is divided into two, the bacterium solution ensured in two quantitative loop is injected simultaneously into measuring cell and reference cell again, after bacterium solution in said two quantitative loop is injected into measuring cell and reference cell, unnecessary bacterium solution in described bacterium solution multiposition valve and pipeline is drained, ensure the bacterium solution not having to remain last time when next time extracts bacterium solution in pipeline；
Step S4: reaction detection, response time is arranged in 1-99min, response time can be set by hand, also the response time can be automatically set by PLC control unit according to the variation tendency of fluorescence intensity in course of reaction, starting stage in the response time, 4th mixing pump and the 3rd mixing pump are blown to measuring cell and reference cell respectively and are stirred, to ensure that bacterium solution is fully reacted；Two photomultiplier tubes are operated in single photon detection pattern, detect the fluorescence intensity in measuring cell and reference cell simultaneously, inject from bacterium solution and terminate up to reaction, complete monitoring detection fluorescence intensity is also sent to PLC control unit, described PLC control unit is according to fluorescence intensity change trend, synthetic determination water quality biological toxicity in initial fluorescent intensity, end fluorescence intensity and course of reaction；
Step S5: discharge opeing is cleaned, after reaction detection process terminates, 4th pond mixing pump and the reversion of the 3rd mixing pump, by the hollow structure of piston rod and leakage fluid dram, described measuring cell and reference cell are emptied, drive motor drives piston rod to move up and down, and described piston rod head seals piston and cleans measuring cell and reference cell inwall；
Step S6: testing procedure is S2-S5, opens water sample selector valve in step S2 process so that measuring cell and reference cell all inject pure water；Calculating negative suppression ratio, it is qualified to be judged to less than 5%；
Step S7: measuring process is S2-S5, opens water sample selector valve in step S2 process so that measuring cell and reference cell all inject pure water, increases in S3 step and injects positive reagent to measuring cell；Calculating positive suppression ratio, it is qualified to be judged to more than 90%.
Compared with prior art, beneficial effects of the present invention:
The present invention has adaptation multi-cultur es, omnidistance detection, certainty of measurement is high, the measurement time is short, bacterium solution consumption is few, without advantages such as cross interference, maintenance are little, meet the demand of online environment monitoring.
Accompanying drawing explanation
Fig. 1 provides the composition frame chart of the automated monitor of a kind of multi-cultur es water quality biological toxicity for the embodiment of the present invention；
Fig. 2 provides the front view of the automated monitor of a kind of multi-cultur es water quality biological toxicity for the embodiment of the present invention
Fig. 3 provides the flow circuit diagram of the automated monitor of a kind of multi-cultur es water quality biological toxicity for the embodiment of the present invention
Fig. 4 be the embodiment of the present invention provide the automated monitor of a kind of multi-cultur es water quality biological toxicity the structure chart of reaction detection unit；
Fig. 5 is the side view of Fig. 4；
Fig. 6 position embodiment of the present invention also provides for the block diagram of the monitoring method of a kind of multi-cultur es water quality biological toxicity.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein is only in order to explain the present invention, is not intended to limit the present invention.
The embodiment of the present invention provides the automated monitor of a kind of multi-cultur es water quality biological toxicity, as it is shown in figure 1, this equipment includes PCL control unit, man-machine interaction unit, stream control unit, recovery storage unit, sampling dosing unit, reaction detection unit；
Described man-machine interaction unit, for interface display and to described recovery storage unit, sampling dosing unit, reaction detection unit parameter be configured；
Described PCL control unit, for receiving the supplemental characteristic of described man-machine interaction unit setting and being transferred to stream control unit, and the testing result of reception reaction detection unit feedback and output display to described man-machine interaction unit；
Described stream control unit, for receive described PCL control unit transmission recovery storage unit, sampling dosing unit, reaction detection unit supplemental characteristic and be issued to successively respectively recovery storage unit, sampling dosing unit, reaction detection unit；Receive the feedback of described recovery storage unit, sampling dosing unit, reaction detection unit simultaneously；
Described recovery storage unit, for receiving and feedback parameter data and the recovery and the preservation that realize bacterium solution to stream control unit；
Specifically, described recovery storage unit is used for recovery and the preservation of bacterium solution, has Programmable Temperature and controls function, can meet different strain recovery and the requirement preserved；
Described sampling dosing unit, for receiving to stream control unit and feedback parameter data and for quantitatively injecting reagent；
Specifically, described sampling dosing unit is for quantitatively injecting the reagent such as bacterium solution, and quantitative accuracy reaches 0.5 μ L, has simultaneous implantation function, solves the inconsistent deviation caused of reagent injection length.
Described reaction detection unit, for receiving to stream control unit and feedback parameter data and for bacterium solution reaction and fluoroscopic examination, omnidistance detection fluorescence intensity change curve and feed back to described PCL control unit in course of reaction.
Specifically, described reaction detection unit integrates bacterium solution reaction and fluoroscopic examination, has Programmable Temperature and controls function, and whole process detects fluorescence intensity change curve in course of reaction.
The embodiment of the present invention also provides for the automated monitor of a kind of multi-cultur es water quality biological toxicity, such as Fig. 2, 3, 4, shown in 5, this equipment includes PCL control unit, man-machine interaction unit 1, stream control unit, recovery storage unit 5, sampling dosing unit, reaction detection unit 9, described PCL control unit, man-machine interaction unit 1, stream control unit, recovery storage unit 5, sampling dosing unit, reaction detection unit 9 is arranged in framework, described PCL control unit and man-machine interaction unit 1, stream control unit, reaction detection unit 9 connects, described stream control unit and recovery storage unit 5, sampling dosing unit, reaction detection unit 9 connects；Described man-machine interaction unit 1 is arranged on the top of framework, and described reaction detection unit 9 and recovery storage unit 5 are arranged on the middle part of framework, and described sampling dosing unit is arranged on middle part and the bottom of framework.
As shown in Figure 3, described recovery storage unit 5 includes 27, two pieces of the 26, second bacterium solution pond, the first bacterium solution pond and is respectively used to control the Programmable Temperature control module 35 of temperature in the 26, second bacterium solution pond 27, the first bacterium solution pond, and the 26, second bacterium solution pond 27, described first bacterium solution pond is connected to first mixing pump the 24, second mixing pump 25.
Described first mixing pump the 24, second mixing pump 25 is piezoelectric pump, for bacterium solution carries out stirring of blowing, extends the bacterium solution life-span.
Described Programmable Temperature controls module 35 and is used for controlling bacterium solution pond temperature, bacterium solution is played recovery and the effect preserved, its heating and cooling parts are semiconductor chilling plate, control mode is PID control, the way of output is PWM output, can arbitrarily arranging temperature curve in (0-45) DEG C, temperature accuracy reaches 0.1 DEG C, meets the recovery of different bacterium solution and preserves requirement.Optimum temperature environment required for (such as Fermi operator, bright bacillus, the Qinghai Vibrion etc.) recovery of research different strain and preservation; the temperature curve parameter of recovery storage unit is set; including recovery temperature value, recovery temperature rate-of-rise, recovery temperature fall off rate, storage temperature curve etc., to adapt to different strain requirement.
As shown in Figure 3, described sampling dosing unit includes water sample injection unit, bacterium solution injection unit, positive injection unit, described water sample injection unit includes multi-channel synchronous peristaltic pump 3, described bacterium solution injection unit includes bacterium solution multiposition valve 4, described positive injection unit includes positive reagent multiposition valve 8, water sample 13, pure water 11, saline 6 are drawn by pipeline in one end of described multi-channel synchronous peristaltic pump 3, and the other end is connected with reaction detection unit 9 and is used for carrying water sample 13, pure water 11, saline 6；Described bacterium solution multiposition valve 4 is connected to the pipeline connected with reaction detection unit 9 with the injection measurement bacterium solution quantitative loop 20 of bacterium solution, reference bacterium solution quantitative loop 21, bacterium solution dosing pump 2, bacterium solution injection pump 22 for quantitative selection, and 26, the second bacterium solution pond 27, the first bacterium solution pond of described recovery storage unit 5 is connected with described bacterium solution multiposition valve 4 through bacterium solution selector valve；Described positive reagent multiposition valve 8 is connected to the pipeline connected with reaction detection unit 9 with the injection positive reagent quantitative loop 18 of positive reagent 10, positive reagent dosing pump 7, positive reagent injection pump 19 for quantitative selection.
The pipeline that described water sample 13, pure water 11 are connected with multi-channel synchronous peristaltic pump 3 is provided with water sample selector valve 12.
Specifically, described water sample injection unit includes multi-channel synchronous peristaltic pump 3 and water sample selector valve 12, for mixing and the injection of water sample 13, pure water 11 and saline 6.When water sample detection, inject pure water 11 and the mixed liquor of saline 6 to the reference cell 14 of reaction detection unit 9, inject water sample 13 and the mixed liquor of saline 6 to the measuring cell 15 of reaction detection unit 9.When feminine gender checking and positive verification, all inject the mixed liquor of pure water 11 and saline 6 to reference cell 14 and measuring cell 15.Described multi-channel synchronous peristaltic pump 3 synchronizes peristaltic pump for four-way, and its entrance connects water sample 13, pure water 11 and saline 6 respectively, and its outlet is injected into measuring cell 15 after water sample 13 and saline 6 being mixed, and is injected into reference cell 14 after pure water 11 and saline 6 being mixed.Each passage of multi-channel synchronous peristaltic pump 3 synchronizes transhipment simultaneously, it may be achieved water sample/brine ratio is accurately consistent with pure water/brine ratio, and ensures that water sample 13 and pure water 11 are simultaneously injected into reaction detection unit 9.Described water sample selector valve 12 is used for switching water sample 13 and pure water 11, when feminine gender checking and positive verification, it is achieved inject pure water 11 in measuring cell 15.
Described bacterium solution injection unit includes bacterium solution multiposition valve 4, reference bacterium solution quantitative loop 21, measures bacterium solution quantitative loop 20, bacterium solution dosing pump 2, bacterium solution injection pump 22, bacterium solution selector valve 23.Described bacterium solution multiposition valve 4 is ten valves, and valve body and spool adopt corrosion resistant material (such as rustless steel, PAKE, PEEK, PPS, PTEF etc.).Described reference bacterium solution quantitative loop 21 and measurement bacterium solution quantitative loop 20 are the PTFE tube of regular length, can adjust the length of pipe according to bacterium solution consumption.Described bacterium solution dosing pump 2 is single channel peristaltic pump, for providing the power extracting bacterium solution.Described bacterium solution injection pump 22 is piezoelectric pump, and for the bacterium solution in quantitative loop is injected into reaction detection unit 9, bacterium solution injection pump 22 is divided into two-way, the measuring cell 15 of the reaction detection unit 9 that can simultaneously the bacterium solution in two quantitative loop be injected separately into and reference cell 14.Measure required bacterium solution only 10 μ L, cost only 3-4 unit every time.
Described positive injection unit includes positive reagent multiposition valve 8, positive reagent quantitative loop 18, positive reagent dosing pump 7, positive reagent injection pump 19.Described positive reagent multiposition valve 8 is six valves, and valve body and spool adopt corrosion resistant material (such as rustless steel, PAKE, PEEK, PPS, PTEF etc.).Described positive reagent quantitative loop 18 is the PTFE tube of regular length, can adjust the length of pipe according to positive reagent consumption.Described positive reagent dosing pump 7 is single channel peristaltic pump, for providing the power extracting positive reagent.Described positive reagent injection pump 19 is piezoelectric pump, for the positive reagent in quantitative loop is injected into the measuring cell 15 of reaction detection unit 9.
As shown in Figure 4,5, described reaction detection unit 9 includes reference cell 14, measuring cell the 15, the 3rd mixing pump the 16, the 4th 17, two photomultiplier tubes 29 of mixing pump, Programmable Temperature control module 35；Described reference cell 14, measuring cell 15 lower end connects the 3rd mixing pump 16 respectively, 4th mixing pump 17, described reference cell 14, measuring cell 15 all includes injection port 28, leakage fluid dram 33, seal piston 31, expand O-ring 30, piston rod 32, drive motor 34, described sealing piston 31 and piston rod 32 are positioned at measuring cell 15 or the inwall of reference cell 14, described expansion O-ring 30 is at piston rod 32 and seals between piston 31, described piston rod 32 is cylindrical hollow structure, for connecting sealing piston 31 and drive motor 34, described photomultiplier tube 29 is arranged on outside measuring cell 15 or reference cell 14, for receiving the fluorescent radiation in measuring cell 15 or reference cell 14；Described measuring cell 15 and reference cell 14 are outside equipped with for providing the Programmable Temperature of stationary temperature environment to control module 35 for measuring cell, reference cell and photomultiplier tube；The bacterium solution multiposition valve 4 of described sampling dosing unit is connected with described reference cell 14, measuring cell 15 respectively, and the positive reagent multiposition valve 8 of described sampling dosing unit is connected with described measuring cell 15.
Specifically, described 3rd mixing pump the 16, the 4th mixing pump 17 is piezoelectric pump, namely can be used for point reference cell 14 and measuring cell 15 is blown and stirred, it is ensured that the abundant reaction of bacterium solution, it is also possible in discharge opeing.
Described Programmable Temperature controls module 35, its heating and cooling parts are semiconductor chilling plate, control mode is PID control, the way of output is PWM output, the temperature value of correspondence can be set according to different strain, thering is provided stationary temperature environment for measuring cell, reference cell and photomultiplier tube, temperature accuracy reaches 0.1 DEG C.
Described sealing piston 31 is PTFE material, is positioned at measuring cell 15 (or reference cell 14) inwall, for plugging meter pond 15 (or reference cell 14) and cleaning measuring cell 15 (or reference cell 14).
Described expansion O-ring 30 material is fluorubber, nitrile rubber or silicone rubber, between position piston rod 32 and sealing piston 31.It is PTFE because sealing piston 31 material, measuring cell 15 (or reference cell 14) material is glass, both thermal coefficient of expansions, therefore when variation of ambient temperature is bigger, seal and there is the phenomenon of leakage between piston 31 and measuring cell 15 (or reference cell 14), utilize the contractility expanding O-ring 30 self, it is ensured that in not temperature lower seal piston 31 and measuring cell 15 (or reference cell 14) tight fit.
Described piston rod 32 is stainless steel material, and for cylindrical hollow structure, its hollow space is used for discharge opeing, and piston rod connects sealing piston 31 and drive motor 34.Under the effect of drive motor 34, band movable sealing piston 31 moves up and down, and reaches to clean the effect of measuring cell 15 and reference cell 14 inwall.
Described photomultiplier tube 29 is operated in single photon detection pattern, photomultiplier tube 29 receives the fluorescent radiation in measuring cell 15 (or reference cell 14), premenstrual put processing and amplifying, level is screened and is removed noise, and convert fluorescence intensity signals to pulse signal, pulse signal is converted to Transistor-Transistor Logic level by voltage comparator, uses FPGA TTL is counted and is sent to PLC control system.When photomultiplier tube 29 is injected into measuring cell 15 and reference cell 14 in bacterium solution, until reaction terminates whole process detection fluorescence intensity.The bacterium solution response time can be arranged in (1-99) min, can arrange the response time by hand, it is possible to automatically set the response time by PLC control system according to the variation tendency of fluorescence intensity in course of reaction.
Described stream control unit drives bacterium solution multiposition valve 4, positive reagent multiposition valve 8, multi-channel synchronous peristaltic pump 3, bacterium solution dosing pump 2, positive reagent dosing pump 7, positive reagent injection pump 19, bacterium solution injection pump 22, the 3rd mixing pump 16, the 4th mixing pump the 17, first mixing pump the 25, second mixing pump 26, Programmable Temperature to control the stream devices such as module 35 by designated program.
The embodiment of the present invention also provides for the monitoring method of a kind of multi-cultur es water quality biological toxicity, and as shown in Figure 6, the method is realized by following steps:
Step S1: prepare before test, the required pure water 11 of preparation, saline 6, positive reagent 10 and bacterium solution, recovery preservation condition and the reaction detection temperature of correspondence are set according to strain used；
Step S2: inject water sample and pure water, multi-channel synchronous peristaltic pump 3 extracts water sample 13, pure water 11 and saline 6 simultaneously, and after water sample 13 and saline 6 being mixed, it is injected into the measuring cell 15 of reaction detection unit 9, the reference cell 14 of reaction detection unit 9 it is injected into after pure water 11 and saline 6 being mixed, four passages of described multi-channel synchronous peristaltic pump 3 synchronize transhipment simultaneously, can ensure that water sample/brine ratio is accurately consistent with pure water/brine ratio, and water sample and pure water are simultaneously injected into reaction detection unit 9；
Step S3: inject bacterium solution, bacterium solution multiposition valve 4 rotates to sample position, bacterium solution dosing pump 2 extracts bacterium solution and to reference bacterium solution quantitative loop 21 and measures bacterium solution quantitative loop 20, described bacterium solution multiposition valve 4 rotates to injection phase, bacterium solution in quantitative loop is injected into described measuring cell 15 and reference cell 14 by bacterium solution injection pump 22 simultaneously, the pipeline three-way interface of described bacterium solution injection pump 22 is divided into two, the bacterium solution ensured in two quantitative loop is injected simultaneously into measuring cell 15 and reference cell 14 again, after bacterium solution in said two quantitative loop is injected into measuring cell 15 and reference cell 14, unnecessary bacterium solution in described bacterium solution multiposition valve 4 and pipeline is drained, ensure the bacterium solution not having to remain last time when next time extracts bacterium solution in pipeline；
Step S4: reaction detection, response time is arranged in 1-99min, response time can be set by hand, also the response time can be automatically set by PLC control unit according to the variation tendency of fluorescence intensity in course of reaction, starting stage in the response time, 4th mixing pump 17 and the 3rd mixing pump 16 are blown to measuring cell 15 and reference cell 14 respectively and are stirred, to ensure that bacterium solution is fully reacted；Two photomultiplier tubes 29 are operated in single photon detection pattern, detect the fluorescence intensity in measuring cell 15 and reference cell 14 simultaneously, inject from bacterium solution and terminate up to reaction, complete monitoring detection fluorescence intensity is also sent to PLC control unit, described PLC control unit is according to fluorescence intensity change trend, synthetic determination water quality biological toxicity in initial fluorescent intensity, end fluorescence intensity and course of reaction；
Step S5: discharge opeing is cleaned, after reaction detection process terminates, 4th pond mixing pump 17 and the 3rd mixing pump 16 are reversed, by the hollow structure of piston rod 32 and leakage fluid dram, described measuring cell 15 and reference cell 14 are emptied, drive motor 34 drives piston rod 32 to move up and down, and described piston rod 32 head seal piston 31 cleans measuring cell 15 and reference cell 14 inwall；
Step S6: testing procedure is S2-S5, opens water sample selector valve 12 in step S2 process so that measuring cell 15 and reference cell 14 all inject pure water 11；Calculating negative suppression ratio, it is qualified to be judged to less than 5%；
Step S7: measuring process is S2-S5, opens water sample selector valve 12 in step S2 process so that measuring cell 15 and reference cell 14 all inject pure water, increases in S3 step and injects positive reagent 9 to measuring cell 15；Calculating positive suppression ratio, it is qualified to be judged to more than 90%.
As shown in Figure 6, the present invention is divided into water sample detection, negative checking and positive verification；Water sample detection is for measuring the bio-toxicity of water sample；Negative checking, for feminine gender checking, is used for judging bacterium solution activity, it is to avoid wrong report；Positive verification, is used for judging bacterium solution sensitivity, it is to avoid fail to report.Negative checking and positive verification carry out once general every day.
The above, be only presently preferred embodiments of the present invention, is not intended to limit protection scope of the present invention.
Claims (7)
1. the automated monitor of a multi-cultur es water quality biological toxicity, it is characterised in that this equipment includes PCL control unit, man-machine interaction unit, stream control unit, recovery storage unit, sampling dosing unit, reaction detection unit；
Described man-machine interaction unit, for interface display and to described recovery storage unit, sampling dosing unit, reaction detection unit parameter be configured；
Described PCL control unit, for receiving the supplemental characteristic of described man-machine interaction unit setting and being transferred to stream control unit, and the testing result of reception reaction detection unit feedback and output display to described man-machine interaction unit；
Described stream control unit, for receive described PCL control unit transmission recovery storage unit, sampling dosing unit, reaction detection unit supplemental characteristic and be issued to successively respectively recovery storage unit, sampling dosing unit, reaction detection unit；Receive the feedback of described recovery storage unit, sampling dosing unit, reaction detection unit simultaneously；
Described recovery storage unit, for receiving and feedback parameter data and the recovery and the preservation that realize bacterium solution to stream control unit；
Described sampling dosing unit, for receiving to stream control unit and feedback parameter data and for quantitatively injecting reagent；
Described reaction detection unit, for receiving to stream control unit and feedback parameter data and for bacterium solution reaction and fluoroscopic examination, omnidistance detection fluorescence intensity change curve and feed back to described PCL control unit in course of reaction.
2. the automated monitor of a multi-cultur es water quality biological toxicity, it is characterized in that, this equipment includes PCL control unit, man-machine interaction unit, stream control unit, recovery storage unit, sampling dosing unit, reaction detection unit, described PCL control unit, man-machine interaction unit, stream control unit, recovery storage unit, sampling dosing unit, reaction detection unit is arranged in framework, described PCL control unit and man-machine interaction unit, stream control unit, reaction detection unit connects, described stream control unit and recovery storage unit, sampling dosing unit, reaction detection unit connects；Described man-machine interaction unit is arranged on the top of framework, and described reaction detection unit and recovery storage unit are arranged on the middle part of framework, and described sampling dosing unit is arranged on middle part and the bottom of framework.
3. the automated monitor of multi-cultur es water quality biological toxicity according to claim 2, it is characterized in that: described recovery storage unit include the first bacterium solution pond, the second bacterium solution pond, two pieces be respectively used to control the Programmable Temperature of temperature in the first bacterium solution pond, the second bacterium solution pond and control module, described first bacterium solution pond, the second bacterium solution pond are connected to the first mixing pump, the second mixing pump.
4. the automated monitor of the multi-cultur es water quality biological toxicity according to Claims 2 or 3, it is characterized in that: described sampling dosing unit includes water sample injection unit, bacterium solution injection unit, positive injection unit, described water sample injection unit includes multi-channel synchronous peristaltic pump, described bacterium solution injection unit includes bacterium solution multiposition valve, described positive injection unit includes positive reagent multiposition valve, water sample, pure water, saline are drawn by pipeline in one end of described multi-channel synchronous peristaltic pump, and the other end is connected with reaction detection unit and is used for carrying water sample, pure water, saline；Described bacterium solution multiposition valve is connected to for quantitative selection and the pipeline injecting the measurement bacterium solution quantitative loop of bacterium solution, reference bacterium solution quantitative loop, bacterium solution dosing pump, bacterium solution injection pump connect with reaction detection unit, and the first bacterium solution pond of described recovery storage unit, the second bacterium solution pond are connected with described bacterium solution multiposition valve through bacterium solution selector valve；Described positive reagent multiposition valve is connected to the pipeline connected with reaction detection unit with the injection positive reagent quantitative loop of positive reagent, positive reagent dosing pump, positive reagent injection pump for quantitative selection.
5. the automated monitor of multi-cultur es water quality biological toxicity according to claim 4, it is characterised in that: the pipeline that described water sample, pure water are connected with multi-channel synchronous peristaltic pump is provided with water sample selector valve.
6. the automated monitor of multi-cultur es water quality biological toxicity according to claim 5, it is characterised in that: described reaction detection unit includes reference cell, measuring cell, the 3rd mixing pump, the 4th mixing pump, two photomultiplier tubes, Programmable Temperature control modules；Described reference cell, measuring cell lower end connect the 3rd mixing pump, the 4th mixing pump respectively, described reference cell, measuring cell all include injection port, leakage fluid dram, sealing piston, expand O-ring, piston rod, drive motor, described sealing piston and piston rod are positioned at the inwall of measuring cell or reference cell, described expansion O-ring is at piston rod and seals between piston, described piston rod is cylindrical hollow structure, for connecting sealing piston and drive motor, described photomultiplier tube is arranged on outside measuring cell or reference cell, for receiving the fluorescent radiation in measuring cell or reference cell；Described measuring cell and reference cell are outside equipped with for providing the Programmable Temperature of stationary temperature environment to control module for measuring cell, reference cell and photomultiplier tube；The bacterium solution multiposition valve of described sampling dosing unit is connected with described reference cell, measuring cell respectively, and the positive reagent multiposition valve of described sampling dosing unit is connected with described measuring cell.
7. the monitoring method of a multi-cultur es water quality biological toxicity, it is characterised in that the method is realized by following steps:
Step S1: prepare before test, the required pure water of preparation, saline, positive reagent and bacterium solution, recovery preservation condition and the reaction detection temperature of correspondence are set according to strain used；
Step S2: inject water sample and pure water, multi-channel synchronous peristaltic pump extracts water sample, pure water and saline simultaneously, and after water sample and saline being mixed, it is injected into the measuring cell of reaction detection unit, the reference cell of reaction detection unit it is injected into after pure water and saline 6 being mixed, four passages of described multi-channel synchronous peristaltic pump synchronize transhipment simultaneously, can ensure that water sample/brine ratio is accurately consistent with pure water/brine ratio, and water sample and pure water are simultaneously injected into reaction detection unit；
Step S3: inject bacterium solution, bacterium solution multiposition valve rotates to sample position, bacterium solution dosing pump extracts bacterium solution and to reference bacterium solution quantitative loop and measures bacterium solution quantitative loop, described bacterium solution multiposition valve rotates to injection phase, bacterium solution in quantitative loop is injected into described measuring cell and reference cell by bacterium solution injection pump simultaneously, the pipeline three-way interface of described bacterium solution injection pump is divided into two, the bacterium solution ensured in two quantitative loop is injected simultaneously into measuring cell and reference cell again, after bacterium solution in said two quantitative loop is injected into measuring cell and reference cell, unnecessary bacterium solution in described bacterium solution multiposition valve and pipeline is drained, ensure the bacterium solution not having to remain last time when next time extracts bacterium solution in pipeline；
Step S4: reaction detection, response time is arranged in 1-99min, response time can be set by hand, also the response time can be automatically set by PLC control unit according to the variation tendency of fluorescence intensity in course of reaction, starting stage in the response time, 4th mixing pump and the 3rd mixing pump are blown to measuring cell and reference cell respectively and are stirred, to ensure that bacterium solution is fully reacted；Two photomultiplier tubes are operated in single photon detection pattern, detect the fluorescence intensity in measuring cell and reference cell simultaneously, inject from bacterium solution and terminate up to reaction, complete monitoring detection fluorescence intensity is also sent to PLC control unit, described PLC control unit is according to fluorescence intensity change trend, synthetic determination water quality biological toxicity in initial fluorescent intensity, end fluorescence intensity and course of reaction；
Step S5: discharge opeing is cleaned, after reaction detection process terminates, 4th pond mixing pump and the reversion of the 3rd mixing pump, by the hollow structure of piston rod and leakage fluid dram, described measuring cell and reference cell are emptied, drive motor drives piston rod to move up and down, and described piston rod head seals piston and cleans measuring cell and reference cell inwall；
Step S6: testing procedure is S2-S5, opens water sample selector valve in step S2 process so that measuring cell and reference cell all inject pure water；Calculating negative suppression ratio, it is qualified to be judged to less than 5%；
Step S7: measuring process is S2-S5, opens water sample selector valve in step S2 process so that measuring cell and reference cell all inject pure water, increases in S3 step and injects positive examination to measuring cell；Calculating positive suppression ratio, it is qualified to be judged to more than 90%.
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