CN101477131A - On-line automatic analyzer for synthetic biological toxicity in water and its use method - Google Patents
On-line automatic analyzer for synthetic biological toxicity in water and its use method Download PDFInfo
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- CN101477131A CN101477131A CNA2009100100691A CN200910010069A CN101477131A CN 101477131 A CN101477131 A CN 101477131A CN A2009100100691 A CNA2009100100691 A CN A2009100100691A CN 200910010069 A CN200910010069 A CN 200910010069A CN 101477131 A CN101477131 A CN 101477131A
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Abstract
The invention relates to a water quality integrated bio-toxicity on-line automatic analyzer and a method for using the same. The analyzer is characterized in that the interior of the analyzer is provided with two temperature control areas, wherein one temperature area at a temperature of between 0 and 4 DEG C mainly comprises a freezing dry powder storing device, a sterilizing device, a freezing dry powder definite quantity control device and a nutrient solution storing device; and the other temperature area at a temperature of between 20 and 25 DEG C mainly comprises a photogenic bacterium activating reaction storing kettle, a buffering liquid storing device, a regulation and control device for water to be tested, a clear water flow rate transmission and control pipeline, a delicate testing device and a rotary long shaft fixing round plate in a dark box, wherein a test tube is assembled on the round plate; the dark box cover is connected with the photogenic bacterium activating reaction storing kettle, the buffering liquid storing device, the control device for water to be tested and an analyzer externally connected washing pipe through the flow rate transmission and control pipeline; and the interior of the dark box is provided with a photoelectric probe. The analyzer has the advantages of on-line test achievement for water quality integrated bio-toxicity, simple operation and high automation degree in the aspect of maintenance, and can obtain reasonable cost performance and be suitable for the on-line monitoring of abominable environment. The analyzer can be used as a single machine, and can also be used as a front-end product of a monitoring network system.
Description
Technical field
The invention belongs to a kind of fwaater resources protection and water quality environment monitoring field, particularly a kind of on-line automatic analyzer for synthetic biological toxicity in water and using method thereof.
Background technology
For the needs of the monitoring that conforms, solve that the bio-toxicity monitoring exists loaded down with trivial details, time-consuming, expense is high and the problem of aspects such as repeated and sensitivity.External always in the method for testing of constantly studying the improvement bio-toxicity, make its quick, sensitive, economy and facility.Begin one's study with the pollution of photobacteria monitoring of environmental from middle nineteen sixties, develop into the measuring technology of comparative maturity to the seventies.Scholar's research application with countries such as the U.S., Britain, the Soviet Union, Japan, Australia is more, and the project that has has been applied for patent, and the Beckmann apparatus company research in this respect of the U.S. has obtained breakthrough progress since the eighties.They have developed special testing tool and the photobacteria test is supporting, are doing many successful researchs aspect the bio-toxicity of using photobacteria method mensuration noxious material.Make this method obtain the popularization of very fast development.In recent years, Chinese scholar is done a lot of work to research and the application of photobacteria, development and production special testing tool, set up photobacteria method bio-toxicity test macro (LB system), for photogen toxicity monitoring lays the foundation applying of China.
But there are following problems in the above-mentioned testing tool that in the past utilized photobacteria to make: supporting as special testing tool and photobacteria test that Beckman company produces, to use but belong in the laboratory, and price is high and too harsh to environment requirement.What the on-line automatic analysis of North America instrument production was adopted is COD and BOD method, also only rests on the bottom that uses in the laboratory.Biology department of domestic East China Normal University and Nanjing Transistor Factory once produced the testing tool that utilizes photobacteria to make, but it is to belong to manual sampling, the manual read fetches data, the intermittence test mode, non-online can only be in the laboratory together with the freezing equipment work in combination.Public Health College, Harbin Medical Univ Hygiene Toxicology teaching and research room once reported and produced acute toxicity photobacteria sensor and acute toxicity high speed tester, design a kind of acute toxicity bacterial luminescence sensor and flow type microcomputer fast measuring environmental contaminants determination of acute toxicity instrument of forming with photobacterium phosphoreum and silicon photo diode, the purpose of qualitative test can only be realized, the toxic grade of online test function and reduced concentration analytical test judgement sewage can not be realized.The CWDZF-I type municipal effluent bio-toxicity on-line automatic analyzer (patent No. is 2004100213555) of Shenyang seven color scientific and technological engineering corporatioies development and production has on-the-spot on-line testing and to advantages such as anti-adverse environments, be specially adapted to the detection of municipal effluent biological synthesis toxicity, but it exists following deficiency and shortcoming:
1, CWDZF-I type in-line analyzer is not owing to there is online activating apparatus, therefore detecting needed photogen each time all is after activating well in advance, be re-introduced in the instrument, because the life-span of bacterium is limited, MaLS is 117 hours, so it is exactly 5 days that follow-on test does not need the time of human intervention the longest, show according to photogen life curve result of detection, the best fluorescent lifetime of photogen is that activation begins 20 hours to 36 hours during this period of time, and CWDZF-I type analysis instrument does not solve best life cycle and prolongs the problem of continuous detecting as far as possible.
2, CWDZF-I type in-line analyzer pipeline uses medical catheter and containers for medical use bottle, utilizes the principle of hanging salt solution to realize the injection of photogen, can appear at pipe interior and other bacteriums occur and strive problem of resource with photogen.Thereby influence the accuracy of testing result.
3, CWDZF-I type in-line analyzer only had a warm area originally, can not finely guarantee that the activity of photogen is in optimum condition, so the luminous intensity deficiency of the photogen in the test process, influence detects quality.
4, the original test cell of CWDZF-I type in-line analyzer adopts the structures such as motor that move up and down photomultiplier, makes test cell rotate with photomultiplier and moves up and down common existence, and the test duration is long, and the data that each test tube is measured are few.
5, after the test cell of CWDZF-I type in-line analyzer rotates, be difficult for accurately in vitro injecting liquid, particularly the head when photomultiplier is directly not corresponding with test tube, because moving up and down of photomultiplier motor can damage photomultiplier.The reliability that influence detects.
Summary of the invention
Purpose of the present invention is intended to solve the above problems, a kind of on-line automatic analyzer for synthetic biological toxicity in water device and using method thereof are provided, realize test freezing preservation and the online activation of luminous bacillus in instrument, the activity that guarantees photogen is in optimum condition, do not need human intervention can detect toxicity automatically and extend to 20 days, the test duration is short, and data acquisition is many, the reliability height that detects, simple in structure, cost is low.
On-line automatic analyzer device provided by the invention: it comprises Instrument shell, the guidance panel that connects electric control gear, the damping fluid hold-up vessel of connection traffic pilot piping, the purge pipe of band flowrate control valve, the fluid cylinder to be measured of connection traffic pilot piping, and be provided with the rotation major axis, the precise testing device of camera bellows and test tube, it is characterized in that: the housing inner chamber is provided with 0 ℃～4 ℃ warm areas and 20 ℃～25 ℃ warm areas, in 0 ℃～4 ℃ warm areas condenser pipe is housed, the external gas gas compressor, in 20 ℃～25 ℃ warm areas electrothermal piece is housed, in two warm areas the temperature acquisition element is set, the temperature acquisition element connects the singlechip CPU of electric control gear; Buffering liquid groove, two cover freeze-dried powders storage quantitative control devices, nutrient solution reservoir are set in 0 ℃～4 ℃ warm areas; Two photogen bacterium activation reaction kettles, activation photogen hold-up vessel, damping fluid hold-up vessel, fluid cylinder to be measured and precise testing device are set in 20 ℃～25 ℃ warm areas; Wherein, buffering liquid groove connects the damping fluid hold-up vessel by the flow control pipeline, the damping fluid hold-up vessel connects two photogen bacterium activation reaction kettles by the flow control efferent duct, two cover freeze-dried powders store quantitative control device and connect corresponding photogen bacterium activation reaction kettles by pipeline respectively, two photogen bacterium activation reaction kettles are by flow control pipeline taking over a job photogen hold-up vessel altogether, and the nutrient solution reservoir connects the photogen bacterium activation reaction kettles by the flow control pipeline; Assembling rotation major axis in the camera bellows of precise testing device, the rotation major axis connects positioning motor, and with several test tubes of assembling on the affixed disk of rotation major axis, each test tube lower end is the delivery pipe of connecting band solenoid valve respectively; Each flow control pipeline passes the camera bellows lid and connects photogen activation hold-up vessel, damping fluid hold-up vessel, fluid cylinder to be measured and clear water pipe respectively; On the annulus of chamber wall and test tube group location, photoelectricity Hall switch and supporting magnet are set respectively, photomultiplier are set at the side of test tube.
The price fixing that above-mentioned freeze-dried powder stores, quantitative control device is provided with the band small opening, the rotating disk that the top assembling of price fixing is driven by positioning motor, rotating disk are provided with a plurality of freeze-dried powders and store hole.
At Instrument shell inner chamber top assembling ultraviolet light.
The using method of on-line automatic analyzer device provided by the invention is as follows:
Photogen activation method: start-up control device, the warm area that stores active photogen freeze-dried powder is controlled at 0 ℃～4 ℃, the activity that keeps the photogen freeze-dried powder, the quantitative control transmission of online activation institute consumption and the storage that realizes the employed volume storage of continuous online activation, nutrient solution; Another warm area in the instrument is remained on 20 ℃～25 ℃, guarantee online activation and the online precision measurement of water quality toxicity of photogen; The method of online activation is to control freeze-dried powder with certain value by the first cover freeze-dried powder storage, quantitative control device, be injected in first photogen bacterium activation reaction kettles by pipeline, by control system control the nutrient solution of certain value and the damping fluid of certain value are injected in this photogen bacterium activation reaction kettles simultaneously, regularly rotate by control system control stirring motor, under 20 ℃～25 ℃ of suitable temperature, process soak time 20 hours, finish activation, the photogen liquid that activation is good is injected in the photogen hold-up vessel, uses in order to test.After time, begin to start and overlap activating apparatus for the second time through a stage activation at the first cover activating apparatus, two cover activating apparatus alternately carry out continuously, and active photogen is injected in the activation photogen hold-up vessel;
The method of test: rotate by control system control rotation major axis, and control is with photogen, damping fluid, three kinds of liquid of water to be measured according to set different concentration proportionings respectively the test tube in camera bellows inject successively, after three kinds of liquid mixing in the test tube are even, the control photodetector detects, rotate by control system control rotation major axis, photodetector is tested the variation of light intensity in each test tube successively, with data storage, be uploaded to host computer, and on guidance panel, show, after detection finishes, open by the terminal solenoid valve of control system control test tube, discharge test liquid in waste liquid tank, open conduit control valve clearly, the rotation major axis rotates, inject the clear water washing successively, bleed off the liquid in it at last.
The present invention compares with prior art has following advantage:
1, realizes the online activation of photogen, thereby solved the problem of grasping the best life cycle of photogen and prolonging continuous detecting as far as possible.Improved the accuracy of testing result.
2, the present invention's sterilization before changing freeze-dried powder, and add malleation at activation flow process pipeline realizes killing the purpose of removing other bacteriums, guarantees that luminous bacillus activates smoothly and keeps better survival condition.
3, the design that two warm areas are set had both solved permanent preservation, online activation, can guarantee that also the activity of photogen is in optimum condition, did not need human intervention can detect toxicity automatically and extended to 20 days.
4, the present invention designs photomultiplier and fixes, and just rotates the test tube group, and the test duration is short, data acquisition is many, reduces the possibility that main devices breaks down.
5, the present invention is provided with locating device, can guarantee still can to realize under the higher situation of detection speed the corresponding one by one of each test tube of test cell and damping fluid, photogen, water to be measured, clear water pipe adapter, with the position of photomultiplier also is corresponding one by one, guarantees the reliability that detects.
6, can realize the online detection of synthetic biological toxicity in water, simple to operate, safeguard the aspect, the automaticity height can access the rational ratio of performance to price so continuity is good.
7, can be applicable to online auto monitoring, utilize it to can be used as unit and use, also can serve as the front end product of monitoring network system than rugged surroundings.Be applicable to on-line monitoring or the analytical instrument of places such as fwaater resources protection, monitoring water environment, biology, environmental protection, petrochemical complex with the band metering.
Description of drawings
Fig. 1 is an instrument general structure synoptic diagram of the present invention.
Fig. 2 is that freeze-dried powder stores, the quantitative control device structural representation.
Fig. 3 is the structural representation of precise testing device.
Fig. 4 is the cut-open view of A-A of Fig. 3.
Fig. 5 is an instrument front panel synoptic diagram of the present invention.
Embodiment
See Fig. 1-4, on-line automatic analyzer implement body structure provided by the invention is as follows:
Setting in 0 ℃～4 ℃ warm areas: buffering liquid groove 27 and uviol lamp 26 are installed on the top of Instrument shell inner chamber; Buffering liquid groove 27 connects damping fluid hold-up vessel 25 by the flow control pipeline 29 of charged magnet valve, buffering liquid groove 27 storage damping fluids can use about 10 days for system, and damping fluid hold-up vessel 25 will cushion drop by damping fluid flow control pipeline 9 and advance part of detecting in vitro.Top in Instrument shell is provided with two cover freeze-dried powders storages, quantitative control device, see Fig. 2, the price fixing 34 that every cover stores, quantitative control device is provided with the band small opening, a small opening that is communicated with freeze-dried powder pipeline 19 (36) is arranged on the price fixing 34, and freeze-dried powder pipeline 19 (36) other ends are connected to photogen bacterium activation reaction kettles 15 (37).The rotating disk 33 that the top assembling of price fixing 34 is driven by positioning motor 21, rotational positioning motor 21 can be controlled the location of rotating disk 33.The hole 23 that 8 storage freeze-dried powders are arranged on the rotating disk 33.On rotating disk 33, cover 31 by the web member fixing seal.Storage hole 23 during original state on the rotating disk 33 and the small opening on the price fixing 33 stagger, positioning motor 21 can clockwise rotate certain angle and made small opening on the next one storage aligned price fixing 34 on the rotating disk 33 each online when activation, and a freeze-dried powder is slipped in the photogen bacterium activation reaction kettles 15 (37) by freeze-dried powder pipeline 19 (36).Nutrient solution reservoir 30 also is set in this warm area, and nutrient solution reservoir 30 connects interior two the photogen bacterium activation reaction kettles 15,37 of 20 ℃～25 ℃ warm areas by the flow control pipeline 20 of charged magnet valve.Control system can be opened flow control solenoid valve quantitative nutrient solution of interpolation in activation still 15 (37) before each activation.Damping fluid hold-up vessel 25 connects two photogen bacterium activation reaction kettles 15 and 37 by the flow control pipeline 17 of charged magnet valve, and control system is opened the flow control solenoid valve to the quantitative damping fluid of bacterium activation reaction kettles 15 (37) interpolations before each activation.
20 ℃～25 ℃ settings that warm area is interior: two photogen bacterium activation reaction kettles 15 and 37 are installed in the middle part in Instrument shell, assemble the stirrer 16 that is driven by motor 35 in the photogen bacterium activation reaction kettles 15 and 37 respectively.Bacterium liquid in the stir-activating reactor mixes it.Two photogen bacterium activation reaction kettles 15 are connected a photogen hold-up vessel 12 by the flow control pipeline 13,14 of charged magnet valve respectively with 37 lower end.After bacterium liquid activated, system can open activation still 15 (37) downside flow solenoid valves and the good bacterium liquid of activation is discharged in the activation back photogen storage tank 12 is used for test.This warm area also is provided with fluid cylinder 40 to be measured, and what fluid cylinder 40 to be measured was provided with charged magnet valve treats liquid-measuring tube 45, by treating liquid-measuring tube 45 drop to be measured is advanced in the test tube of part of detecting.The clear water pipe 44 of charged magnet valve also is set simultaneously.Precise testing device is installed in bottom in Instrument shell, precise testing device structure: see Fig. 1, Fig. 3, Fig. 4, assembling rotation major axis 46 in the camera bellows 51, rotation major axis 46 connects positioning motor 42, rotation major axis 46 affixed disks 49, be uniformly distributed along the circumference on the disk 49 and assemble six test tubes, each test tube lower end is the delivery pipe 2 of connecting band solenoid valve 4 respectively, and the solenoid valve 4 and the delivery pipe 2 of each test tube lower end are fixedly connected on the annulus 3.Each flow control pipeline passes camera bellows lid 43 and connects photogen activation hold-up vessel 12, damping fluid reservoir 25, fluid cylinder to be measured 40 and clear water pipe 44 respectively; On the annulus of chamber wall and test tube group location, photoelectricity Hall switch 52 and supporting magnet 5 are set respectively, photomultiplier 50 are set at the side of test tube.
Pressurized air input pipe 39 is set in system, in damping fluid hold-up vessel 25, nutrient solution reservoir 30, two photogen bacterium activation reaction kettles 15 and 37, photogen storage tank 12 and connected traffic transport controlling plumbing fixtures, apply the pressure of 1～2Kg, so that the intrusion that prevents extraneous bacterium in the reactivation process to be provided.
Activated partial mainly comprises: freeze-dried powder storage, quantitative control device, photogen bacterium activation reaction kettles 15 and 37, nutrient solution reservoir 30, damping fluid hold-up vessel 25, activation back photogen storage tank 12, temperature acquisition parts and corresponding pipeline and solenoid valve.
Part of detecting mainly comprises:, rotation major axis 46, test be with test tube group, photomultiplier 50, fluid cylinder to be measured 40 and photoelectricity Hall switch.
See Fig. 5, instrument front panel of the present invention is provided with power switch 58, UV-lamp switch 59, LCDs 60, button 61 and liquid run-down pipe to be measured outlet 55, liquid input pipe interface 56 to be measured, pressurized air input pipe interface 57, clear water input pipe interface 62.
The system testing principle is as follows: system changes the position of test tube by the rotation of control motor 42.Low level signal of photoelectricity Hall switch 52 outputs when test tube disk 49 is in initial position, otherwise output is high level signal.Before each test beginning and when testing, detection all can be done to the output signal of photoelectricity Hall switch 52 by system.If signal is high level then adopts rational location algorithm rotary electric machine 42 to come to make it get back to initial position for test tube dish location.Thereby being used in combination, photoelectricity Hall switch 52 and stepper motor can locate the location that test tube disk 49 is realized test tube well.Photomultiplier 50 is positioned at a side of No. 4 test tubes 48, and it can gather the light signal that photogen sends, and light signal is converted to faint electric signal for the control system acquisition process.When test tube disk 49 was in initial position, No. 1 test tube 6 was aimed at 43, No. 4 test tubes of 8, No. 2 test tubes of interpolation bacterium liquid pipe, 7 aligning damping fluid pipes, 9, No. 3 test tubes, 47 aligning clear water pipes, 48 alignings and is treated liquid-measuring tube 45, and No. 4 test tubes have also been aimed at photomultiplier 50 simultaneously.Add bacterium liquid pipe 8 and link to each other with activation back photogen storage tank 12, system adds quantitative bacterium liquid by opening and closing interpolation bacterium flow quantity control electromagnetic valve in test tube.Damping fluid pipe 9 links to each other with damping fluid storage tank 25, adds quantitative damping fluid by the Control Flow solenoid valve in test tube.Clear water pipe 44 links to each other with water pump pipe or running water pipe and supplies water at any time, and system adds clear water to test tube by switch flow control solenoid valve, is used to wash test tube.Treat that liquid-measuring tube 45 links to each other with fluid cylinder 40 to be measured, system can come to add quantitative liquid to be measured by the switching of flow control solenoid valve in test tube.Fluid cylinder to be measured links to each other with water pump, and water pump can send liquid to be measured always in fluid cylinder to be measured be up-to-date to keep the liquid to be measured in the fluid cylinder to be measured.The upside of fluid cylinder 40 to be measured has overflow vent to link to each other with run-down pipe 41, and it is excessive with the liquid to be measured that prevents fluid cylinder to be measured that liquid to be measured can flow to other place by run-down pipe 41.There is wastewater trough 1 in delivery pipe 2 bottoms, and the waste liquid that tests in the test tube all will be discharged in the wastewater trough 1 with the liquid that cleans test tube.
The using method of on-line automatic analyzer device provided by the invention is as follows:
The online reactivation process of system is as follows:
1, uviol lamp 26 is opened about half an hour before each activation and is used for sterilization processing is carried out in activated partial container, equipment and housing upside space, is beneficial to the growth of photogen.
2, online activation begins front opening flow control solenoid valve and add a certain amount of damping fluid in buffering flow container 25.In test tube, add quantitative damping fluid by the flow control solenoid valve during test.Damping fluid mainly is a liquid volume in the adding container, and to reach the purpose of variable concentrations, damping fluid is to the not influence of luminous intensity of luminous bacillus.
3, start-up control device is controlled at 0 ℃～4 ℃ with the warm area that stores active photogen freeze-dried powder, and another warm area in the instrument is remained on 20 ℃～25 ℃.
4, control freeze-dried powder with certain value by the freeze-dried powder quantitative control device, be injected in first photogen bacterium activation reaction kettles 15 by freeze-dried powder pipeline 19, simultaneously the nutrient solution of certain value and the damping fluid of certain value are injected in this photogen bacterium activation reaction kettles, the control stirring motor regularly rotates, under 20 ℃～25 ℃ of suitable temperature, the bacterium liquid activation in the beginning bacterium activation reaction kettles.
5, control freeze-dried powder with certain value by the freeze-dried powder quantitative control device after 16 hours, be injected in second bacterium activation reaction kettles 37 by freeze-dried powder pipeline 19, simultaneously the nutrient solution of certain value and the damping fluid of certain value are injected in this photogen bacterium activation reaction kettles, the control stirring motor regularly rotates, under 20 ℃～25 ℃ of suitable temperature, the bacterium liquid activation in the beginning bacterium activation reaction kettles.
6,, after bacterium liquid in first bacterium activation reaction kettles 15 activates 20 hours, it is discharged in the activation back photogen storage tank 12 in order to the test use again through 4 hours.
7, repeat to do the activation of bacterium liquid through in first bacterium activation reaction kettles 15, adding raw material after 12 hours again.
8, after 4 hours after bacterium liquid activation in second bacterium activation reaction kettles 37 20 hours, wherein bacterium liquid is discharged in the activation back photogen storage tank 12.
9, the bacterium liquid that repeated to do in the bacterium activation reaction kettles 37 to the quantitative raw materials such as freeze-dried powder, damping fluid and nutrient solution of second activation still, 37 interpolations after 12 hours activates.
Two bacterium activation reaction kettles alternately repeat above-mentioned activation procedure, are the cycle alternately two activation stills to be done the operation of adding raw material and discharging bacterium liquid to get final product with 32 hours.
The test process of system is as follows:
1, adds a certain amount of bacterium liquid, damping fluid and liquid to be measured to first test tube earlier.Motor 42 changes 60 degree clockwise, adds three kinds of quantitative liquid to second test tube.Motor successively rotates 60 degree 5 times, corresponding three kinds of liquid that add corresponding amount in the test tube of correspondence 5 times.Thereby make three kinds of liquid that all added corresponding amount in 6 test tubes.Liquid has added rear motor and has rotated counterclockwise 300 degree.
2, reorientate and make test tube disk 49 get back to initial position.The photogen luminous intensity of gathering in the test tube by photomultiplier 50 clockwise rotates positioning motor 60 degree 5 times then earlier.The moving photogen luminous intensity of once gathering corresponding test tube of revolution.After light intensity in 6 test tubes had all been gathered, motor rotated counterclockwise 300 degree.Reorientate test tube disk 49 and make it get back to initial position, gather light intensity in each test tube once more according to top mode then.Repeat collecting flowchart 10 times.
3, bleed off waste liquid in the test tube, in test tube, add clear water and clean test tube 2 times.
Show after the class of pollution of water quality toxicity is calculated by the special software bag and be stored in the corresponding database.Measurement data shows and is stored in the program of single-chip microcomputer at working site LED, and realizes serial communication by 485/232 change-over circuit, and field data is sent to remote control room, both can realize single-set operation, also can realize the networking operation.
The corresponding relation of luminous bacillus luminous intensity parameter and measured matter toxicity index, be to set up relevant toxicity analytical database, and the photobacteria toxicity test method of percentiles grade classification principle that adopts U.S. BULICH.A.A to propose is carried out the toxic grade division by testing.The metric of toxicity intensity is as follows:
(1) relative light intensity: relative light intensity=sample light intensity/contrast light intensity * 100%
(2) press down the light rate relatively: press down light rate=contrast light intensity-sample light intensity/contrast light intensity * 100% relatively
(3) EC50:EC50 makes the toxic concentration of luminous intensity minimizing 50% or the dilute concentration (or extension rate) of sewage.
Photobacteria test method of percentiles grade classification
| EC50 or LC50 (percentage concentration) | The toxicity rank | Grade |
| ＜25% 25-75% 75-100%〉100% or can not ask EC50 | Strong malicious little poison is nontoxic | I II III IV |
Claims (5)
1, a kind of on-line automatic analyzer for synthetic biological toxicity in water, it comprises Instrument shell, the guidance panel that connects electric control gear, the buffering flow container of connection traffic pilot piping, the purge pipe of band flowrate control valve, the fluid cylinder to be measured of connection traffic pilot piping, and be provided with the rotation major axis, the precise testing device of camera bellows and test tube, it is characterized in that: the housing inner chamber is provided with 0 ℃～4 ℃ warm areas and 20 ℃～25 ℃ warm areas, in 0 ℃～4 ℃ warm areas condenser pipe is housed, the external gas gas compressor, in 20 ℃～25 ℃ warm areas electrothermal piece is housed, in two warm areas the temperature acquisition element is set, the temperature acquisition element connects the singlechip CPU of electric control gear; Buffering liquid groove, two cover freeze-dried powders storage quantitative control devices, nutrient solution reservoir are set in 0 ℃～4 ℃ warm areas; Two photogen bacterium activation reaction kettles, activation photogen hold-up vessel, damping fluid hold-up vessel, fluid cylinder to be measured and precise testing device are set in 20 ℃～25 ℃ warm areas; Wherein, buffering liquid groove connects the damping fluid hold-up vessel by the flow control pipeline, the damping fluid hold-up vessel connects two photogen bacterium activation reaction kettles by the flow control efferent duct, two cover freeze-dried powders store quantitative control device and connect corresponding photogen bacterium activation reaction kettles by pipeline respectively, two photogen bacterium activation reaction kettles are by flow control pipeline taking over a job photogen hold-up vessel altogether, and the nutrient solution reservoir connects the photogen bacterium activation reaction kettles by the flow control pipeline; Assembling rotation major axis in the camera bellows of precise testing device, the rotation major axis connects positioning motor, and with several test tubes of assembling on the affixed disk of rotation major axis, each test tube lower end is the delivery pipe of connecting band solenoid valve respectively; Cover respectively by flow control pipeline connection photogen activation hold-up vessel, damping fluid hold-up vessel, water regulation device to be measured and the external purge pipe of analyser at camera bellows; On chamber wall and test tube group positioning disc, photoelectricity Hall switch and supporting magnet are set respectively, photomultiplier are set at the side of test tube.
2, on-line automatic analyzer for synthetic biological toxicity in water according to claim 1, it is characterized in that: freeze-dried powder stores the price fixing that quantitative control device is provided with the band small opening, the rotating disk that the top assembling of price fixing is driven by positioning motor, rotating disk are provided with a plurality of freeze-dried powders and store hole.
3, on-line automatic analyzer for synthetic biological toxicity in water according to claim 1 is characterized in that: at Instrument shell inner chamber top assembling ultraviolet germicidal lamp.
4, a kind of using method as on-line automatic analyzer for synthetic biological toxicity in water is characterized in that:
The photogen activation method: the start-up control device, the warm area that stores active photogen freeze-dried powder is controlled at 0 ℃～4 ℃, keep the activity of photogen freeze-dried powder, the quantitative control transmission of online activation institute consumption, the storage of nutrient solution; Another warm area in the instrument is remained on 20 ℃～25 ℃, guarantee online activation and the online precision measurement of water quality toxicity of photogen; The method of online activation is to store quantitative control device by the first cover freeze-dried powder to control freeze-dried powder with certain value, be injected in first photogen bacterium activation reaction kettles by pipeline, by control system control the nutrient solution of certain value and the damping fluid of certain value are injected in this photogen bacterium activation reaction kettles simultaneously, regularly rotate by control system control stirring motor, under 20 ℃～25 ℃ of suitable temperature, process soak time 20 hours, finish activation, the photogen liquid that activation is good is injected in the photogen hold-up vessel, uses in order to test.After time, begin to start and overlap activating apparatus for the second time through a stage activation at the first cover activating apparatus, two cover activating apparatus alternately carry out continuously, and active photogen is injected in the activation photogen hold-up vessel;
The method of test: rotate by control system control rotation major axis, and control is with photogen, damping fluid, three kinds of liquid of water to be measured according to set different concentration proportionings respectively the test tube in camera bellows inject successively, after three kinds of liquid mixing in the test tube are even, the control photodetector detects, rotate by control system control rotation major axis, photodetector is tested the variation of light intensity in each test tube successively, with data storage, be uploaded to host computer, and on guidance panel, show, after detection finishes, open by the terminal solenoid valve of control system control test tube, discharge test liquid in waste liquid tank, open conduit control valve clearly, the rotation major axis rotates, inject the clear water washing successively, bleed off the liquid in it at last.
5, the using method of on-line automatic analyzer for synthetic biological toxicity in water according to claim 4, it is characterized in that: in damping fluid hold-up vessel, nutrient solution reservoir, two photogen bacterium activation reaction kettles, photogen storage tank and connected traffic transport controlling plumbing fixtures, apply the pressure of 1～2Kg, so that the intrusion that prevents extraneous bacterium in the reactivation process to be provided.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101936910A (en) * | 2010-08-04 | 2011-01-05 | 聚光科技(杭州)股份有限公司 | Method and device for analyzing water toxicity |
| CN104048954A (en) * | 2014-06-23 | 2014-09-17 | 宇星科技发展(深圳)有限公司 | Water toxicity detector and water toxicity detection system |
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2009
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| CN101936910A (en) * | 2010-08-04 | 2011-01-05 | 聚光科技(杭州)股份有限公司 | Method and device for analyzing water toxicity |
| CN101936910B (en) * | 2010-08-04 | 2013-02-20 | 聚光科技(杭州)股份有限公司 | Method for analyzing water toxicity |
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