CN111700972A

CN111700972A - Acne-removing traditional Chinese medicine composition and preparation method thereof

Info

Publication number: CN111700972A
Application number: CN202010734644.9A
Authority: CN
Inventors: 王俊赫; 曹桂云; 黄蒙蒙; 孟兆青; 李樱; 段迁远; 李家昌
Original assignee: Shandong Hongjitang Pharmaceutical Group Co ltd
Current assignee: Shandong Hongjitang Pharmaceutical Group Co ltd
Priority date: 2020-07-28
Filing date: 2020-07-28
Publication date: 2020-09-25

Abstract

The invention provides a traditional Chinese medicine composition with an acne removing function and application thereof in the field of cosmetics. The traditional Chinese medicine composition comprises the following traditional Chinese medicine components in parts by weight: 8-16 parts of mung bean, 1-5 parts of mint, 3-10 parts of rheum officinale, 2-8 parts of honeysuckle, 1-6 parts of fructus forsythiae, 3-10 parts of rhizoma bletillae, 1-8 parts of dandelion, 3-9 parts of wild chrysanthemum flower and 1-6 parts of liquorice. The invention is characterized in that natural herbaceous plants are used as main raw materials, and the invention has the effects of bacteriostasis, anti-inflammation, oil control and acne removal.

Description

Acne-removing traditional Chinese medicine composition and preparation method thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a traditional Chinese medicine composition with antibacterial, anti-inflammatory, oil control and acne removal effects and a preparation method thereof.

Background

Acne is commonly found in adolescent men and women, and is also called as "comedo" or "acne". Research shows that the incidence rate of whelk is 9.4 percent in the global scope, and the whelk becomes the eighth global pandemic. Wherein, the incidence rates of African Americans, Hispanic Americans and Asians are respectively 37%, 32% and 30%. An epidemiological study involving 83008 Chinese people showed that the overall prevalence of whelks in China was 39.2%. The common part of the traditional Chinese medicine composition is the face, the traditional Chinese medicine composition is easy to attack repeatedly, the appearance of a patient is greatly influenced, in addition, the patient can be caused to generate negative emotions of anxiety and depression, and the learning, social contact and the like of the patient are influenced.

At present, the pathogenesis of whelk is related to factors such as high androgen level of the body, vigorous sebaceous gland secretion, microbial infection of propionibacterium acnes and the like, secondary inflammation and the like. When the expression of androgen is increased, the sebaceous gland can secrete more grease, meanwhile, the hair follicle of a patient is hyperkeratotic, and the cutin is accumulated in the infundibulum of the hair follicle and is combined with the grease to form a mixture to block the conduit of the sebaceous gland, so that the blockage of a grease plug is formed. In addition, the redundant grease can also be used as a culture medium for some bacteria and fungi, and a large number of anaerobic bacteria such as propionibacterium acnes parasitize in the hair follicle duct to breed in a large quantity, so that fatty acid is generated, the skin function is damaged, and the inflammatory reaction is caused. According to related research, TNF-α、IgG、IL-1βIL-2, IL-4, IL-8, IL-10 are elevated, IFN-γDecreased and positively correlated with skin lesion severity.

The current acne medications are divided into systemic and topical medications. Systemic administration mainly includes antibiotic, antiandrogen and isotretinoin. The local external treatment mainly comprises the following steps: topical antibacterial therapy, retinoids, hydroxy acids, antiandrogens, and hydrogen peroxide. The above acne-removing products have the following problems: 1. easily cause skin allergy and has great skin irritation; 2. the blockage is easy to cause, and the excretion of the skin is difficult to discharge after the external medicine is applied for a long time, so that the inflammation of hair follicles, such as face blisters, pimples, even melanosis or black spots, is caused. 3. Hormone products easily cause osteoporosis, cause resistance reduction of the body, electrolyte imbalance and other serious side effects; 4. heavy metals are added, so that the health-care food has harm to the nervous system, the bone hematopoiesis effect, the digestive system, the reproductive system and the like of human beings; 5. generally, the traditional Chinese medicine is used for treating a certain factor of whelk, and has limited curative effect and easy relapse.

In conclusion, the need for an acne-removing preparation which can make up for the defects of the prior art and has good effect is needed in the field.

Disclosure of Invention

The invention aims to provide an acne-removing traditional Chinese medicine composition and a preparation method thereof, the traditional Chinese medicine composition treats acne from multiple links of bacteriostasis, oil control, anti-inflammation and the like, has outstanding curative effect, high effective rate and small toxic and side effects, and the preparation method has the advantages of no damage to the medicine effect, large medicine-carrying amount, stable medicine effect, high effective component, simple and convenient preparation and use and the like.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the acne-removing traditional Chinese medicine composition comprises the following components in parts by weight: 8-16 parts of mung bean, 1-5 parts of mint, 3-10 parts of rhubarb, 2-8 parts of honeysuckle, 1-6 parts of fructus forsythiae, 3-10 parts of rhizoma bletillae, 1-8 parts of dandelion, 3-9 parts of wild chrysanthemum flower and 1-6 parts of liquorice.

The preparation method comprises the following steps:

(1) extracting mung bean, mint, rhubarb, honeysuckle, forsythia, bletilla, dandelion, wild chrysanthemum and liquorice with water in a refluxing way for 2 times, wherein the mass volume ratio g/ml of the extracted raw material medicines to the water for the first time is 1: 8-1: 15, extracting for 1-2 hours, and filtering by using a sieve with 80-200 meshes to obtain a first filtrate; the mass volume ratio g/ml of the bulk drugs and water extracted for the second time is 1: 6-1: 12, extracting for 0.5 to 1 hour, and filtering by using a sieve with 80 to 200 meshes to obtain a second filtrate;

(2) mixing the two filtrates, and centrifuging to obtain extractive solution;

(3) concentrating the extractive solution to 0.2-5 g/ml;

(4) directly packaging the extractive solution or drying, pulverizing and packaging.

Or the preparation method comprises the following steps:

mixing semen Phaseoli Radiati, herba Menthae, radix et rhizoma Rhei, flos Lonicerae, fructus forsythiae, rhizoma Bletillae, herba Taraxaci, flos Chrysanthemi Indici, and Glycyrrhrizae radix, pulverizing, and sieving.

The pharmacology of the drugs related to the traditional Chinese medicine extract is as follows:

mung bean: mung bean of genus Phaseolus of family Leguminosae (Phaseolus)Phaseolus radiatusL.) of the seed. Sweet in nature and cool; has effects of refreshing, removing toxic substances, promoting urination, and improving eyesight.

Mint: is dried aerial parts of Mentha haplocalyx of Labiatae. Pungent in flavor and cool in nature. It enters lung and liver meridians. Has effects in dispelling pathogenic wind and heat, refreshing mind, relieving sore throat, promoting eruption, dispersing stagnated liver qi, and promoting qi circulation.

Rhubarb: is prepared from Rheum palmatum (Rumex palmatum) of PolygonaceaeRheum palmatumL. and Tang Gu Da Huang (radix Et rhizoma Rhei)Rheum tanguticumMaxim, ex Balf.) or rhubarb (rhubarb horsetails)Rheum officinaleBaill) dried roots and rhizomes. Bitter and cold in nature. It enters spleen, stomach, large intestine, liver and pericardium meridians. Has the effects of purging, eliminating pathogenic accumulation, clearing away heat, purging pathogenic fire, cooling blood, removing toxic substances, removing blood stasis, dredging channels, promoting diuresis, and eliminating jaundice.

Honeysuckle flower: is prepared from Lonicera japonica Thunb of Caprifoliaceae familyLonicera japonicaThunb.) or a flower with an initial bloom. Sweet in nature and cold in nature. Has effects of clearing away heat and toxic materials, and dispelling pathogenic wind and heat.

Fructus forsythiae: is prepared from fructus forsythiae (Forsythia suspensa (Thunb.) Vahl, Forsythia of Oleaceae)Forsythia suspensa(Thunb.) Vahl). Bitter in property and slightly cold in nature. Has the effects of clearing away heat and toxic materials, relieving swelling, dispersing pathogenic accumulation, and dispelling pathogenic wind and heat.

And (3) common bletilla pseudobulb: is bletilla striata of Orchidaceae (Bletilla striata(Thunb.) Reichb. f.) dried tubers. Bitter, sweet, astringent and slightly cold in nature. Has effects of astringing, stopping bleeding, eliminating swelling, and promoting granulation.

Dandelion: is prepared from herba Taraxaci (Compositae)Taraxacum mongolicumHand. -Mazz.), Taraxacum alkalium (A.) (Taraxacum borealisinenseKitam.) or dried whole herbs of several plants of the same genus. Bitter, sweet and cold in nature. Has the effects of clearing away heat and toxic materials, relieving swelling, dispersing pathogenic accumulation, inducing diuresis, and treating stranguria.

Wild chrysanthemum flower: is prepared from wild chrysanthemum flower of CompositaeChrysanthemum indicumL.) dried capitula. Bitter and pungent in property, slightly cold in nature. Has the effects of clearing away heat and toxic materials, purging pathogenic fire, and calming liver.

Licorice root: is prepared from Glycyrrhrizae radix (Glycyrrhiza uralensis Fisch. of Leguminosae)Glycyrrhiza uralensisFisch, liquorice root (a mixture of fruit of swelling and fruit of Chinese licorice)Glycyrrhiza inflataBat.) or Glycyrrhiza glabra (Glycyrrhiza glabraL.) dried roots and rhizomes. Sweet and neutral in nature. Has the effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, eliminating phlegm and relieving cough, relieving spasm and pain, and harmonizing the medicines.

The main purpose of adding the liquorice in the composition is to match and harmonize the ingredients with other medicinal flavors so as to achieve the purpose of improving the overall curative effect. The traditional Chinese medicine composition has the total effective rate of acne removal up to 100 percent and high safety.

According to different using ways, the acne-removing traditional Chinese medicine composition can be applied to the face as powder, and the extract can also be prepared into other external skin care preparations, such as facial masks, facial cleansers, essence solutions, face creams, perfumed soaps and the like, and is not limited to the formulations.

Compared with the prior art, the invention has the beneficial effects that:

(1) the traditional Chinese medicine components have the effects of clearing away heat and toxic materials, reducing swelling, resolving hard mass, removing toxicity and promoting granulation, the compatibility is more reasonable, the effect of treating the acne is achieved from multiple links of bacteriostasis, oil control, anti-inflammation and the like, and the effective rate and the safety are higher;

(2) the traditional Chinese medicine composition and the skin care preparation prepared from the same have the advantages of simple method, low production cost, convenient use and high consumer acceptance.

Drawings

FIG. 1 the effect of the mask sample on inhibiting the secretion of lipids.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The used medicinal materials, reagents or instruments are not indicated by manufacturers, and all the medicinal materials, the reagents or the instruments are conventional products which can be obtained by commercial purchase.

While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.

Example 1

Taking 160g of mung bean, 50g of mint, 100g of rhubarb, 80g of honeysuckle, 10g of fructus forsythiae, 100g of rhizoma bletillae, 60g of dandelion, 90g of wild chrysanthemum and 10g of liquorice, uniformly mixing, crushing and sieving by a 200-mesh sieve.

When in use, the face is cleaned firstly, then the powder is mixed into paste by boiled water, when the face is warm, the yoghourt and the honey are added for mixing uniformly, the face is applied, and the face is washed off after 20 minutes.

Example 2

Taking 80g of mung bean, 50g of mint, 40g of rheum officinale, 80g of honeysuckle, 50g of fructus forsythiae, 30g of rhizoma bletillae, 15g of dandelion, 40g of wild chrysanthemum and 60g of liquorice, uniformly mixing, crushing and sieving by a 200-mesh sieve.

Example 3

200g of mung bean, 40g of mint, 100g of rhubarb, 60g of honeysuckle, 60g of fructus forsythiae, 120g of rhizoma bletillae, 60g of dandelion, 100g of wild chrysanthemum and 40g of liquorice are cleaned and extracted twice under reflux. Adding 10 times of water for the first time, refluxing for 1h, and filtering with a 80-mesh sieve to obtain a first filtrate. Adding 8 times of water for the second time, refluxing for 0.5h, and filtering with a 80-mesh sieve to obtain a second filtrate. The two filtrates were combined, centrifuged at 3000r and the supernatant was taken. Concentrating the supernatant to obtain extractive solution with concentration of 1 g/ml.

The preparation method of the traditional Chinese medicine mask comprises the following steps:

dissolving 4g of sodium hyaluronate, 5g of trehalose and 5g of xanthan gum in 100ml of purified water at 90-95 ℃ to prepare transparent colloidal solution for later use. And (3) adding the mixed solution of sodium hyaluronate, trehalose and xanthan gum, 250ml of glycerol, 4g of dipotassium glycyrrhizinate and 20g of sodium benzoate into the traditional Chinese medicine extracting solution, and uniformly stirring to obtain the mask liquid.

Adding the facial mask solution into non-woven fabric or other medium to obtain the finished facial mask.

Example 4

150g of mung bean, 20g of mint, 60g of rhubarb, 30g of honeysuckle, 20g of fructus forsythiae, 50g of rhizoma bletillae, 30g of dandelion, 50g of wild chrysanthemum flower and 20g of liquorice are cleaned and extracted twice under reflux. Adding 10 times of water for the first time, refluxing for 2h, and filtering with a 150-mesh sieve to obtain a first filtrate. Adding 10 times of water for the second time, refluxing for 1h, and filtering with a 150-mesh sieve to obtain a second filtrate. The two filtrates are combined, centrifuged at 5000r, and the supernatant is taken. Concentrating the supernatant to obtain extractive solution equivalent to 0.5g/ml of raw materials, and drying to obtain brown powder combined extract.

dissolving the Chinese medicinal powder, 6g sodium hyaluronate, 6g trehalose, and 8g xanthan gum in 100ml purified water of 90-95 deg.C to obtain transparent colloidal solution. Adding 300ml of glycerol, 6g of dipotassium glycyrrhizinate and 25g of sodium benzoate, and uniformly stirring to obtain the mask liquid.

Example 5

25g of mung bean, 5g of mint, 9g of rheum officinale, 4g of honeysuckle, 6g of fructus forsythiae, 10g of rhizoma bletillae, 6g of dandelion, 12g of wild chrysanthemum and 5g of liquorice are washed and extracted twice under reflux. Adding 12 times of water for the first time, refluxing for 1.5h, and filtering with a 100-mesh sieve to obtain a first filtrate. Adding 10 times of water for the second time, refluxing for 1h, and filtering with a 100-mesh sieve to obtain a second filtrate. The two filtrates are combined, centrifuged at 5000r, and the supernatant is taken. Concentrating the supernatant to obtain extractive solution with concentration of 5 g/ml.

The preparation method of the traditional Chinese medicine facial cleanser comprises the following steps:

160g of lauric acid, 320g of myristic acid, 240g of palmitic acid, 400g of stearic acid, 40g of ethylene glycol distearate and 20g of PEG-120 distearate were mixed, stirred and heated to 80-85 ℃ to dissolve the components. Dissolving 800g of glycerol, 120g of acrylate copolymer and 240g of sodium hydroxide by using a proper amount of deionized water, adding the solution, and stirring to obtain a paste. Mixing the Chinese medicinal extract with 200g propylene glycol, dissolving, cooling to 45-50 deg.C, adding the above paste, adding appropriate amount of essence and antiseptic, and stirring.

Example 6

10g of mung bean, 4g of mint, 3g of rheum officinale, 5g of honeysuckle, 3g of fructus forsythiae, 6g of rhizoma bletillae, 5g of dandelion, 8g of wild chrysanthemum and 3g of liquorice are washed and extracted twice under reflux. Adding 12 times of water for the first time, refluxing for 1.5h, and filtering with a 150-mesh sieve to obtain a first filtrate. Adding 10 times of water for the second time, refluxing for 1h, and filtering with a 150-mesh sieve to obtain a second filtrate. The two filtrates are combined, centrifuged at 5000r, and the supernatant is taken. Concentrating the supernatant to obtain extractive solution with concentration of 2 g/ml.

The preparation method of the soap comprises the following steps:

taking 100g of lauric acid, 10g of EDTA-4Na, 1g of hexadecanol, 5g of titanium dioxide, 1g of essence, a proper amount of pigment and preservative, stirring uniformly, adding 10ml of glycerol and a proper amount of deionized water, refining, grinding and extruding into strips.

Comparative example 1

Unlike example 2, the used traditional Chinese medicine did not include licorice.

Efficacy test of the invention

1. Oil control effect of product

Test method

Subjects with oil inclusion rates above 30% totaled 20, 15 women and 5 men. The subjects used a mask (prepared in example 3) prepared with the traditional Chinese medicine composition every night, measured 3-5 times in the center of the forehead by the technician using SK-8 type skin oil tester on

days

0, 4, 7, and 14 am, respectively, and averaged.

Test results

And evaluating the oil control effect of the mask by using the fat inhibition rate as an index. Fat-reduction rate = (fat content after use of mask-initial fat content)/initial fat content. The results are shown in fig. 1, and the fat-suppression rate of the subjects after using the mask is negative and is significantly different from that before using the mask. After the mask is used, the grease secretion of a subject is greatly reduced.

2. Bacteriostatic effect of the product

Test method

On the culture medium inoculated with the test strain, a sterilized steel tube is used for punching the culture medium, the culture medium is carefully picked out to form a round hole, 80 mu L of facial mask liquid (prepared in example 3) is injected into the hole, pre-diffusion is carried out for 2h at 4 ℃, culture is carried out for 24h at 37 ℃, the size of a bacteriostatic circle is measured, the test is carried out for 3 times in parallel, and the test result is expressed by 'x +/-s'. The principle is that the sample penetrates to reach a certain concentration after a certain time by utilizing the diffusion and penetration effect of the sample in a culture medium, the bacterial growth is inhibited, and a sterile transparent area called a bacteriostatic zone is formed.

Test results

The main pathogens of acne, propionibacterium acnes and staphylococcus aureus, are selected in the test to explore the antibacterial activity of the mask liquid. The results show that the facial mask liquid has good inhibition effect on propionibacterium acnes and staphylococcus aureus, and has significant statistical difference compared with the normal saline (P<0.01) and the bacteriostatic diameters of the antibacterial agent on the 2 acne pathogenic bacteria are respectively (2.06 +/-0.14) cm and (1.53 +/-0.11) cm, and the results are shown in a table 1.

TABLE 1 bacteriostatic action of facial mask liquid

Note: as compared with the saline groupP<0.01。

3. Anti-inflammatory effect of the product

1) The product has effect of inhibiting rat foot swelling caused by crossed vegetable gum

Test method

10 Wistar rats were randomly assigned to 2 groups, model group, and facial mask fluid group (prepared in example 3). The volume of the right hind paw of all rats was measured as the normal value before the model was made. Rats were anesthetized with 10% chloral hydrate and then injected with 0.04 mL of 1% carrageenan into the right hind toe of the rats to cause inflammation. The facial mask liquid group rats are continuously smeared with facial mask liquid, and the surfaces of the right hind toes are kept moist. After 20min, the facial mask liquid on the surfaces of the toes of the rats is cleaned. The model group was smeared with an equal amount of physiological saline. Measuring the volume of the right hind toe of the rat at 1h, 2h, 3 h, 4h and 5h after the model making, and taking the swelling degree as a detection index. Swelling = volume of right hind paw with inflammation-volume of right hind paw before inflammation.

Test results

Acute inflammatory reactions such as redness and swelling begin to appear on the right hind paw of the rat 1h after carrageenan injection, the peak is reached in about 3 h, and the swelling of the hind paw gradually subsides in 4 h. The facial mask liquid can obviously reduce the foot swelling degree of rats, and the results are shown in table 2.

TABLE 2 Effect of facial mask solution on carrageenan-induced footpad swelling model in rats

Comparing with model groupP<0.05, denotesP<0.01。

2) The product can be used for treating IL-1 in rat tumescent footβEffects of IL-6 and PGE2

Test method

After measuring the volume of the right hind paw of the rat, the rat is killed by removing the cervical vertebra, the injured tissue of the right hind paw is cut off on ice, weighed, and homogenized in ice bath by adding 9 times of physiological saline. Homogenate 3000 r.min^-1Centrifuging at low temperature for 15 min, respectively collecting supernatant to determine IL-1βIL-6 and PGE2, according to the kit instructions.

Test results

IL-1 in toe tissue of model group rats compared with normal control groupβThe content of IL-6 and PGE2 is obviously increased, and the application of the facial mask liquid can obviously reduce IL-1βIL-6 and PGE2 contentsP<0.05 orP<0.01). The results are shown inTable 3.

TABLE 3 facial mask solution for rat toe IL-1βEffects of IL-6 and PGE2

Comparing with model groupP<0.05, denotesP<0.01。

4. Acne removing clinical test

Test method

The test screens the subjects with the variola skin with the total lesion number of 10-30 faces to carry out clinical test. A total of 40 subjects were enrolled and randomized into 2 groups, and each of example 3 and comparative example was given for evaluation of acne-removing efficacy.

Cleaning face of the subject at night every day, applying the face mask on the face, standing for 15-20min, taking off the face mask, and cleaning the face with clear water. The subjects were observed for regression of acne on

days

7, 14, 21 and 28 after application of the mask. Wherein, the effective rate is more than 60% of whelk fading, the effective rate is more than 30% of whelk fading, the ineffective rate is less than 30% of whelk fading or whelk fading is aggravated, and the total effective rate = the effective rate + the effective rate.

Test results

As can be seen from the table, the acne treatment of the facial mask is higher than that of the traditional treatment method, the curative effect of the sample group is better than that of the comparative sample group, and no adverse reaction occurs in the test subjects in the test process, which indicates that the facial mask is safe to use for a long time.

TABLE 4 therapeutic effect of acne-removing facial mask on acne

The traditional Chinese medicine composition provided by the application has the advantages of better compatibility, high total effective rate, high safety and obvious curative effect on whelk.

Claims

1. The acne-removing traditional Chinese medicine composition is characterized by comprising the following raw materials in percentage by weight:

8-16 parts of mung bean

1-5 parts of mint

3-10 parts of rhubarb

2-8 parts of honeysuckle

1-6 parts of weeping forsythia

3-10 parts of bletilla striata

1-8 parts of dandelion

3-9 parts of wild chrysanthemum flower

1-6 parts of liquorice.

2. The acne-removing traditional Chinese medicine composition according to claim 1, wherein the traditional Chinese medicine composition is an external preparation.

3. The acne-removing traditional Chinese medicine composition according to claim 2, wherein the external preparation is a solution or a powder.

4. The acne-removing traditional Chinese medicine composition according to claim 1, 2 or 3, wherein the preparation method of the traditional Chinese medicine composition is as follows:

(2) mixing the two filtrates, and centrifuging to obtain extractive solution;

(3) concentrating the extractive solution to 0.2-5 g/ml;

5. The acne-removing traditional Chinese medicine composition according to claim 1, 2 or 3, wherein the preparation method of the traditional Chinese medicine composition is as follows:

6. The acne-removing traditional Chinese medicine composition according to claim 1, 2 or 3, wherein the traditional Chinese medicine composition is used for bacteriostasis.

7. The acne-removing traditional Chinese medicine composition according to claim 1, 2 or 3, wherein the traditional Chinese medicine composition is used for anti-inflammation.

8. The acne-removing traditional Chinese medicine composition according to claim 1, 2 or 3, wherein the traditional Chinese medicine composition is used for controlling oil.

9. An acne removing traditional Chinese medicine composition according to claim 1, 2 or 3, characterized in that the composition can be used as a cosmetic component.