CN111700170A - Feed additive for improving pork quality and application thereof - Google Patents

Feed additive for improving pork quality and application thereof Download PDF

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Publication number
CN111700170A
CN111700170A CN202010467828.3A CN202010467828A CN111700170A CN 111700170 A CN111700170 A CN 111700170A CN 202010467828 A CN202010467828 A CN 202010467828A CN 111700170 A CN111700170 A CN 111700170A
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China
Prior art keywords
feed additive
feed
pork
aspartic acid
lactobacillus
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CN202010467828.3A
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Chinese (zh)
Inventor
何流琴
李铁军
吴健
冯艳忠
周锡红
王晨昱
李建中
印遇龙
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Hunan Normal University
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Hunan Normal University
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Priority to CN202010467828.3A priority Critical patent/CN111700170A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

A feed additive for improving pork quality, comprising D-aspartic acid, Lactobacillus cellobiosus, Bifidobacterium animalis, Lactobacillus reuteri, and a carrier. Preferably, the feed additive comprises: 50 wt% of D-aspartic acid, 10-15 wt% of lactobacillus cellobiosus, 10-15 wt% of bifidobacterium animalis, 10-15 wt% of lactobacillus reuteri and the balance of soybean meal as a carrier. The invention also provides application of the feed additive, and the feed additive is added into pork pig feed for improving pork quality. The feed additive does not contain any antibiotic component, and has the advantages of no toxicity, no drug residue, no drug resistance, no pollution and the like.

Description

Feed additive for improving pork quality and application thereof
Technical Field
The application belongs to the technical field of animal nutrition, and particularly relates to a feed additive for improving pork quality and application thereof.
Background
China is a big country for pork production, and the pork yield accounts for about 50% of the total world production. Pork is one of the main sources of meat food in the dietary structure of people in China, the pork consumption is the most dominant in meat consumption, and the health of everyone is influenced by the quality of the pork.
With the improvement of living standard, the attention and the demand of consumers on high-quality pork are increasing. Animal nutrition is one of important factors affecting pork quality, and pork products produced by using feed and/or feed additives containing antibiotic ingredients affect the quality and safety of pork due to antibiotic residues.
Therefore, the development of a novel feed additive without antibiotic components has very important significance for pork pig breeding and pork production.
Disclosure of Invention
The invention aims to provide a feed additive for improving pork quality, which comprises D-aspartic acid, lactobacillus cellobiosus, bifidobacterium animalis, lactobacillus reuteri and a carrier.
Preferably, the feed additive comprises: 50 wt% of D-aspartic acid, 10-15 wt% of lactobacillus cellobiosus, 10-15 wt% of bifidobacterium animalis, 10-15 wt% of lactobacillus reuteri and the balance of soybean meal as a carrier.
Preferably, the feed additive comprises: 50 wt% of D-aspartic acid, 10 wt% of lactobacillus cellobiosus, 10 wt% of bifidobacterium animalis, 10 wt% of lactobacillus reuteri and 20 wt% of soybean meal.
Preferably, the feed additive comprises: 50 wt% of D-aspartic acid, 15 wt% of lactobacillus cellobiosus, 15 wt% of bifidobacterium animalis, 15 wt% of lactobacillus reuteri and 5 wt% of soybean meal.
Preferably, the feed additive comprises: 50 wt% of D-aspartic acid, 10 wt% of lactobacillus cellobiosus, 15 wt% of bifidobacterium animalis, 10 wt% of lactobacillus reuteri and 15 wt% of soybean meal.
The invention also provides application of any one of the feed additives, and the feed additive is added into pork pig feed for improving the pork quality.
Preferably, the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 30mg/kg to 6000mg/kg, depending on the application.
Preferably, the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 50mg/kg, depending on the application.
Preferably, the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 500mg/kg, depending on the application.
Preferably, the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 5000mg/kg, depending on the application.
The feed additive takes D-aspartic acid, lactobacillus cellobiosus, bifidobacterium animalis and lactobacillus reuteri as main functional components, the D-aspartic acid as an amino acid component can participate in biosynthesis and other metabolic activities in organisms as a signal molecule, and the synergistic effect of the three probiotic components can help to regulate the flora balance in the organisms, enhance the immunity of the organisms, improve the disease resistance and promote the digestion and absorption of food. The feed additive is suitable for being mixed into pork pig feed and is used for promoting the growth and development of pigs and/or improving the quality of pork. When the additive is mixed into the feed of pork pigs at low dosage, the additive can effectively promote the growth and development of the pork pigs and increase the deposition of muscle fat. When the additive is mixed into pork pig feed at a high dosage, the pork quality can be obviously improved. In addition, the feed additive does not contain any antibiotic component, and has the advantages of no toxicity, no drug residue, no drug resistance, no pollution and the like.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The present application provides a feed additive for improving pork quality, comprising: d-aspartic acid, Lactobacillus cellobiosus (Lactobacillus cellobiosus), Bifidobacterium animalis (Bifidobacterium), Lactobacillus reuteri (Lactobacillus reuteri) and a carrier. The carrier is preferably soybean meal.
The feed additive comprises: 50 wt% of D-aspartic acid, 10-15 wt% of lactobacillus cellobiosus, 10-15 wt% of bifidobacterium animalis, 10-15 wt% of lactobacillus reuteri and the balance of soybean meal as a carrier.
According to one embodiment of the invention, the feed additive comprises: 50 wt% of D-aspartic acid, 10 wt% of lactobacillus cellobiosus, 10 wt% of bifidobacterium animalis, 10 wt% of lactobacillus reuteri and 20 wt% of soybean meal.
According to one embodiment of the invention, the feed additive comprises: 50 wt% of D-aspartic acid, 15 wt% of lactobacillus cellobiosus, 15 wt% of bifidobacterium animalis, 15 wt% of lactobacillus reuteri and 5 wt% of soybean meal.
According to one embodiment of the invention, the feed additive comprises: 50 wt% of D-aspartic acid, 10 wt% of lactobacillus cellobiosus, 15 wt% of bifidobacterium animalis, 10 wt% of lactobacillus reuteri and 15 wt% of soybean meal.
According to one embodiment of the invention, the feed additive comprises: 50 wt% of D-aspartic acid, 12 wt% of lactobacillus cellobiosus, 10 wt% of bifidobacterium animalis, 15 wt% of lactobacillus reuteri and 13 wt% of soybean meal.
The invention also provides application of the feed additive, and the feed additive is added into pork pig feed to improve pork quality.
According to one embodiment of the invention, in the application of the feed additive described above, any of the feed additives described above is incorporated into a pork pig feed such that the content of D-aspartic acid in the pork pig feed is 50-5000mg/kg, preferably 50mg/kg, 500mg/kg or 5000 mg/kg.
To help better understand the technical solution of the present invention, the following examples are provided to illustrate the preparation of the feed additive of the present invention and its effect on pork quality.
Example 1: preparation of feed additive
The feed additive formulation of this example is: 50 wt% of D-aspartic acid, 10 wt% of lactobacillus cellobiosus, 10 wt% of bifidobacterium animalis, 10 wt% of lactobacillus reuteri and 20 wt% of soybean meal. .
Respectively and independently culturing Lactobacillus cellobiosus, Bifidobacterium animalis and Lactobacillus reuteri by using a correspondingly optimized culture medium by adopting a conventional technology in the field, separating thalli from the culture, and drying the separated thalli in a vacuum freeze drying manner to prepare dry powder of various thalli, wherein the viable bacteria content in the dry powder of the Lactobacillus cellobiosus is more than or equal to 2 × 109CFU/g, the viable bacteria content in animal bifidobacterium dry powder is more than or equal to 1 × 109CFU/g, the viable bacteria content in the lactobacillus reuteri dry powder is more than or equal to 2 × 1010CFU/g。
Weighing D-aspartic acid, cellobiose lactobacillus dry powder, animal bifidobacterium dry powder, lactobacillus reuteri dry powder and soybean meal powder according to the weight ratio, uniformly mixing to prepare the feed additive, and storing at 4 ℃ for later use.
Example 2: influence of feed additive on pork quality
Selecting 40 healthy three-way-hybrid (Du multiplied by long multiplied by big) weaned piglets with the average weight of 10.04 plus or minus 0.32kg, randomly dividing the piglets into four groups according to the principle that the weights of the halves of the male piglets and the female piglets are similar, and repeating 10 times in each group and 1 pig in each group. Wherein the control group was fed a conventional basal diet, the first test group (test group I) was fed a basal diet incorporating 0.01 wt% of the feed supplement of example 1, the second test group (test group II) was fed a basal diet incorporating 0.1 wt% of the feed supplement of example 1, and the third test group (test group III) was fed a basal diet incorporating 1 wt% of the feed supplement of example 1. The test period was 12 weeks.
The daily ration components are prepared according to the nutritional requirement standard of 10-30kg, 30-50kg and 50-90kg of body weight in the American NRC (2012) with the nutritional level.
The test pigs are fed in a single fence, and the conditions of all the fence groups are basically consistent. A pre-test period of 7 days was carried out before the test formally started, and basal diet was fed to each group during the pre-test period. During the test period, the test pigs are fed with the powder for 3 times every day according to the conventional feeding management, the free drinking water is realized, the piglets are fed for 07:30, 12:00 and 18:30, the free drinking water and the feeding are realized, and the principle that no residual materials exist in a trough is taken. The colony house was cleaned 2 times a day and kept clean.
After the beginning of the formal test, each group was fed with different daily rations. The colony house was cleaned 2 times a day and kept clean. The whole colony house is naturally ventilated, and all colony houses are disinfected irregularly in the culture period.
Test pigs were slaughtered at week 12 and examined for pork quality-related indicators. The results are shown in Table 1.
TABLE 1
Figure BDA0002513264830000041
Figure BDA0002513264830000051
As can be seen from table 1, the third test group showed significantly increased soleus muscle a (45min) and lean meat percentage compared to the control group. Meanwhile, the third test group also showed significantly increased dorsal muscle a (24h), dorsal muscle b (24h), dorsal muscle system hydraulic power, dorsal muscle gluteal rate, but the dorsal muscle shear force showed significantly decreased. The first test group showed significantly increased dorsal muscle a (24h), dorsal muscle system hydraulic power, carcass length, backfat thickness and leaf fat weight, but significantly decreased dorsal muscle shear compared to the control group.
This result indicates that different levels of feed additives have different effects on pork pigs when the feed additive according to the invention is applied to the feed of pork pigs. The feed additive with low dosage can effectively promote the growth and development of pigs and increase the deposition of muscle fat, and the feed additive with high dosage can help improve the quality of pork.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It will be apparent to those skilled in the art that improvements and modifications may be made without departing from the principles of the invention and are intended to be within the scope of the invention.

Claims (10)

1. A feed additive for improving pork quality, comprising D-aspartic acid, Lactobacillus cellobiosus, Bifidobacterium animalis, Lactobacillus reuteri, and a carrier.
2. The feed additive of claim 1, wherein:
the feed additive comprises: 50 wt% of D-aspartic acid, 10-15 wt% of lactobacillus cellobiosus, 10-15 wt% of bifidobacterium animalis, 10-15 wt% of lactobacillus reuteri and the balance of soybean meal as a carrier.
3. The feed additive of claim 2, wherein:
the feed additive comprises: 50 wt% of D-aspartic acid, 10 wt% of lactobacillus cellobiosus, 10 wt% of bifidobacterium animalis, 10 wt% of lactobacillus reuteri and 20 wt% of soybean meal.
4. The feed additive of claim 2, wherein:
the feed additive comprises: 50 wt% of D-aspartic acid, 15 wt% of lactobacillus cellobiosus, 15 wt% of bifidobacterium animalis, 15 wt% of lactobacillus reuteri and 5 wt% of soybean meal.
5. The feed additive of claim 2, wherein: the feed additive comprises: 50 wt% of D-aspartic acid, 10 wt% of lactobacillus cellobiosus, 15 wt% of bifidobacterium animalis, 10 wt% of lactobacillus reuteri and 15 wt% of soybean meal.
6. Use of the feed additive according to any one of claims 1 to 5, incorporated into a pork pig feed for improving the quality of pork.
7. Use according to claim 6, characterized in that: the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 30mg/kg to 6000 mg/kg.
8. Use according to claim 6, characterized in that: the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 50 mg/kg.
9. Use according to claim 6, characterized in that: the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 500 mg/kg.
10. Use according to claim 6, characterized in that: the feed additive is incorporated into the pork pig feed in an amount such that the content of D-aspartic acid in the pork pig feed is 5000 mg/kg.
CN202010467828.3A 2020-05-28 2020-05-28 Feed additive for improving pork quality and application thereof Pending CN111700170A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4529595A (en) * 1983-01-13 1985-07-16 The Salk Institute For Biological Studies GRF Analogs
CN106615771A (en) * 2016-11-16 2017-05-10 漳州傲农牧业科技有限公司 Premix for raising growth hormone level in blood of growing-finishing pigs
CN110292103A (en) * 2019-07-02 2019-10-01 广东省农业科学院动物科学研究所 A kind of live pig nonreactive feed and preparation method thereof
CN110463840A (en) * 2019-09-05 2019-11-19 湖南师范大学 It is a kind of improve sow production performance feed addictive and its application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4529595A (en) * 1983-01-13 1985-07-16 The Salk Institute For Biological Studies GRF Analogs
CN106615771A (en) * 2016-11-16 2017-05-10 漳州傲农牧业科技有限公司 Premix for raising growth hormone level in blood of growing-finishing pigs
CN110292103A (en) * 2019-07-02 2019-10-01 广东省农业科学院动物科学研究所 A kind of live pig nonreactive feed and preparation method thereof
CN110463840A (en) * 2019-09-05 2019-11-19 湖南师范大学 It is a kind of improve sow production performance feed addictive and its application

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
YUYING LI等: ""D - and L -Aspartate regulates growth performance, inflammation and intestinal microbial community in young pigs"", 《FOOD AND FUNCTION》 *
夏嗣廷等: ""D-天冬氨酸生理功能及其在畜禽生产中的应用"", 《动物营养学报》 *
王倩: ""酸性氨基酸水平对断奶仔猪生长性能的影响研究"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *
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