CN111676161A - Method for rapidly and accurately preparing microbial fertilizer special for sweet potatoes and prepared microbial fertilizer - Google Patents

Method for rapidly and accurately preparing microbial fertilizer special for sweet potatoes and prepared microbial fertilizer Download PDF

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CN111676161A
CN111676161A CN202010557874.2A CN202010557874A CN111676161A CN 111676161 A CN111676161 A CN 111676161A CN 202010557874 A CN202010557874 A CN 202010557874A CN 111676161 A CN111676161 A CN 111676161A
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bacterial
hundred million
microbial fertilizer
sweet potato
sweet potatoes
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温洪宇
张�浩
泮佳佳
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Jiangsu Normal University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/10—Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12—Granules or flakes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The invention discloses a method for quickly and accurately preparing a special microbial fertilizer for sweet potatoes and the prepared microbial fertilizer, wherein the preparation method comprises the following steps: (1) sampling sweet potato planting land soil, extracting total DNA of rhizosphere soil bacterial communities from soil samples, performing high-throughput sequencing on a V4-V5 hypervariable region formed by bacterial community DNA16S rRNA genes on an Illumina HiSeq PE250 platform, evaluating 16S rRNA gene composition, and analyzing to obtain the rhizosphere of the sweet potato rhizosphere soil bacterial communities; (2) and preparing liquid bacterial manure or solid bacterial manure containing the sweet potato rhizosphere soil bacterial community core bacteria according to the analysis result. The preparation method provided by the invention can be used for rapidly and accurately detecting the rhizosphere soil of the sweet potatoes, specifically providing the lacking bacteria, constructing a healthy and reasonable bacterial community, and improving the yield of the sweet potatoes and the sustainability of a sweet potato planting system.

Description

Method for rapidly and accurately preparing microbial fertilizer special for sweet potatoes and prepared microbial fertilizer
Technical Field
The invention relates to the technical field of microbial fertilizers, and particularly relates to a method for quickly and accurately preparing a microbial fertilizer special for sweet potatoes and the prepared microbial fertilizer.
Background
Sweet potato is an important grain, feed and industrial raw material in the global scope, has the characteristics of high yield, high efficiency, drought resistance, strong adaptability and the like, and has the planting area and the yield which are only inferior to those of rice, wheat and corn. In recent years, with the implementation of policies such as adjustment of planting structures in China and exploitation of underground water pressure in northern areas, the planting area of sweet potatoes is increased year by year, and large-scale production is accelerated. The sweet potato is one of the leading forces of Chinese agricultural production, and the production yield and ecological planting of the sweet potato are very critical. Therefore, how to improve the yield of the sweet potatoes and the sustainability of the sweet potato planting system is particularly important.
The nutrients are the material basis for the sprouting of the sweet potato blocks and the growth of the sweet potato seedlings, and the nutrients are mainly supplemented by chemical fertilizers and organic fertilizers in the current sweet potato planting process. The microbial fertilizer is a novel fertilizer biological product which leads crops to obtain required nutrients through the life activities of active bacteria, and is one of fertilizers in agricultural production. Rhizosphere soil bacteria play an important role in nutrient circulation, organic matter decomposition, soil aggregate stabilization, symbiosis with plants, pathogenic interaction and the like. Rhizosphere soil bacteria can promote nutrient absorption of sweet potatoes, hormone production and prevent diseases and pests. The rapid and accurate understanding of the composition of the rhizosphere soil bacterial community can help people to prepare corresponding bacterial manure aiming at the bacterial flora lacking in sweet potato planting, so that the yield of the sweet potatoes and the sustainability of a sweet potato planting system are improved.
Disclosure of Invention
The invention aims to provide a method for quickly and accurately preparing a microbial fertilizer special for sweet potatoes, which solves the problems of sweet potato yield reduction caused by poor structure of rhizosphere soil bacterial communities and soil hardening and fertility reduction caused by overuse of chemical fertilizers by reconstructing the rhizosphere soil bacterial communities planted in the sweet potatoes.
The invention also aims to provide the special microbial fertilizer for the sweet potatoes, which is prepared by the method, so that nutrients are provided for sweet potato planting, and the yield of the sweet potatoes is increased.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a method for rapidly and accurately preparing a microbial fertilizer special for sweet potatoes comprises the following steps:
(1) sampling sweet potato planting land soil, extracting total DNA of rhizosphere soil bacterial communities from soil samples, performing high-throughput sequencing on a V4-V5 hypervariable region formed by bacterial community DNA16S rRNA genes on an Illumina HiSeq PE250 platform, evaluating 16S rRNA gene composition, and analyzing to obtain the core genus of the sweet potato rhizosphere soil bacterial communities;
(2) and preparing liquid bacterial manure or solid bacterial manure containing the sweet potato rhizosphere soil bacterial community core bacteria according to the analysis result.
Further, the preparation method of the liquid bacterial manure comprises the following steps: pure strains of each core bacterium of sweet potato rhizosphere soil bacterial community are obtained by adopting a pure culture technology, and liquid bacterial manure is obtained by mixing according to a certain proportion after enlarged culture.
Preferably, the bacterial composition in the liquid bacterial manure comprises 1.5-3.5 hundred million pseudomonas, 0.5-2.5 hundred million bacillus halophilus, 0.5-2.5 hundred million pseudomonas and 0.5-2.5 hundred million nitrosomonas.
Preferably, the preparation method of the liquid bacterial manure is as follows: under the aseptic condition, respectively inoculating nitrosomonas, pseudomonas, halophilous myxobacter, gemonas and pseudoxanthomonas to an LB culture medium, and culturing at the constant temperature of 28-37 ℃ for 24-36 h; uniformly mixing the cultured bacterial suspension according to a certain volume ratio to ensure that the final concentration is as follows: 1.5-3.5 hundred million Pseudomonas pseudoxanthans per milliliter, 0.5-2.5 hundred million Nitrosomonas nitrosata per milliliter, 0.5-2.5 hundred million Bacillus halophilus per milliliter, and 0.5-2.5 hundred million Bacillus pumilus per milliliter.
Further, the preparation method of the solid bacterial manure comprises the following steps: pulverizing the base material into coarse powder as excipient; mixing the excipient and the mixed solution of the sweet potato rhizosphere soil bacterial community and each core bacterial strain in the volume ratio of 1.5-2.0: 1, mixing, adsorbing a bacterial solution by using a base material, and adding water with a corresponding volume to enable the temperature to reach 30-40%; and uniformly stirring the base material and the bacterial liquid, further crushing, sieving, granulating and packaging to obtain the microbial inoculum.
Preferably, the base stock is roughly processed crop straws or bran.
Preferably, the solid bacterial manure comprises the following bacteria: 1.0-2.5 hundred million Pseudomonas pseudoxanthans/gram, 0.5-2.0 hundred million Bacillus halophilus/gram, 0.5-2.0 hundred million Bacillus, and 0.5-2.0 hundred million Nitrosomonas.
The invention also provides the microbial fertilizer special for sweet potatoes prepared by the method.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the method, a high-throughput sequencing technology is adopted to quickly and accurately analyze the composition of the rhizosphere soil bacterial community for sweet potato planting, corresponding strains can be added in a targeted manner to reconstruct the rhizosphere soil bacterial community for sweet potato planting, the problem of sweet potato yield reduction caused by poor structure of the rhizosphere soil bacterial community is solved, and the sweet potato yield and the sustainability of a sweet potato planting system are improved;
2. the sweet potato planting microbial fertilizer prepared by the invention has biological control effects of killing insects, secreting antibiotics, regulating plant growth substances and the like, can improve the microenvironment of sweet potato rhizosphere soil, and reduces the occurrence of crop diseases and insect pests;
3. the sweet potato planting microbial fertilizer prepared by the invention can obviously enhance the soil fertility, can reduce the fertilizer dosage, relieves the soil hardening phenomenon caused by long-term application of the fertilizer, further improves the soil structure, has no pollution, and accords with the green life concept pursued by modern people.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1
1.1 planting site and Material
The planting site is selected at the institute of life science of the university of Jiangsu Master (34 degrees 20 'N, 117 degrees 19' E; 35 meters above sea level). The region belongs to a typical temperate zone monsoon climate, the annual average temperature is 14.5 ℃, and the day and night temperature difference is about 8 ℃. The average annual precipitation is 862mm, and 67% of the annual precipitation is mainly distributed in 6-9 months. It is noteworthy that the significant diurnal temperature difference provides good growth conditions for sweet potatoes.
Xushu 32, Xushu 22 and purple sweet potato 5 are the main varieties of Xuzhou region sweet potato and are provided by the national sweet potato center. Xushu 32 is derived from Xushu 55-2 and Hongdong, which are parents of the national sweet potato center, and has higher yield than Xushu 22 and purple sweet potato 5. Xushu 22 has high yield and good adaptability, and is obtained by taking Yushu No. 7 and Sushu No. 7 as parents. The purple sweet potato 5 is produced by natural pollination in 2013, the female parent is commercial sweet potato 19, and the male parent is random sweet potato.
1.2 planting and sampling
The research on the correlation between the sweet potato variety and the growth stage and the bacterial community is established at the test point in 2016, 6 months. The test consisted of investigating the bacterial community changes of 3 varieties at three growth stages, with three replicates. All sweet potato seedlings were sown in 27 plastic pots (diameter 20 cm, depth 10 cm) filled with soil (mesh size: 2mm) taken from a nearby vegetable garden and mixed with white sand at a volume ratio of 3: 1. The pH value of the soil is 5.4, the organic matter concentration of the soil is 61.95g/Kg, and the determination is carried out by adopting a specific gravity method. In each basin, the same amount of fermented chicken manure dry powder is added. During the planting period (S1), the vigorous growth period (S2), and the harvest period (S3), watering was performed approximately once a week.
Test soil samples were collected separately (2 repetitions per cycle) at S1, S2, and S3. On each sampling date, about 30g of rhizosphere soil was collected directly from the root surface (depth 5cm) (soil that dropped naturally and loosely adhered to the root was discarded, and soil that tightly adhered to the root was collected). The samples were stored in a-80 ℃ freezer and subsequently subjected to 16S rRNA detection. Sweet potato samples were collected at the mature stage (153 days after planting) of sweet potatoes in 10 months and 31 days. The stem and root were used as one sample, and the stem length (S1, S2, S3) and the dry weight of the root (S3) were measured, respectively. The relationship between stem length and growth stage (differential test using one-way anova) is shown in table 1.
TABLE 1 Stem growth at different growth stages of sweet potatoes
Figure BDA0002544978660000041
1.3 DNA extraction and 16S rRNA sequencing
Extracting rhizosphere soil bacterial flora RNA from the soil sample by using a PowerSoil total RNA isolation kit, and then extracting bacterial flora total DNA by using an RNA PowerSoil DNA elution kit. To assess the composition of rhizosphere soil bacterial communities, the V4-V5 hypervariable region of the genomic composition of the environmental DNA16S rRNA was sequenced. The 16S rRNA gene encodes archaea and bacterial small subunit ribosomal RNA, and contains 10 conserved regions and 9 hypervariable regions. Hypervariable regions tend to exhibit higher nucleotide diversity while conserved regions exhibit little or no nucleotide diversity. 16S rRNA gene composition was assessed by high-throughput sequencing of the Universal archaea and bacterial primers 515F-907R against the hypervariable region of the 16S rRNA gene V4-V5 on the Illumina HiSeq PE250 platform. The sequencing results are shown in tables 2 and 3 after splicing and assembling.
TABLE 2 diversity and abundance ratio of rhizosphere soil bacteria community at different growth stages (gate level, data in table are percentages)
Figure BDA0002544978660000051
TABLE 3 diversity and abundance ratios of rhizosphere soil bacterial communities at different growth stages (genus level, data in table are percentages)
Figure BDA0002544978660000061
Figure BDA0002544978660000071
From tables 2 and 3, it can be seen that: at the phylum level, the rhizosphere soil bacterial communities of xu potato 32, xu potato 22 and purple potato 5 take proteobacteria, bacteroidetes and acidobacteroidetes as the dominant phyla. Wherein the proteobacteria are most abundant, and account for more than 40% of Xushu 32, Xushu 22 and purple sweet potato 5; on the genus level, nitrosomonas, granulomonas, halophilus, pseudomonas and pseudoxanthomonas are the predominant genera. Therefore, in the planting of three sweet potatoes, the 5 genera are the core components of the sweet potato rhizosphere soil bacterial community and are closely related to the growth of the sweet potatoes.
1.4 preparation of liquid bacterial manure for sweet potato planting:
(11) culturing: under the aseptic condition, respectively inoculating nitrosomonas, pseudomonas, halophilous myxobacter, gemonas and pseudoxanthomonas to an LB culture medium, and culturing at the constant temperature of 28-37 ℃ for 24-36 h;
(12) mixing: uniformly mixing the cultured bacterial suspension according to a certain volume ratio to ensure that the final concentration is as follows: 1.5-3.5 hundred million Pseudomonas pseudoxanthans per milliliter, 0.5-2.5 hundred million Nitrosomonas nitrosata per milliliter, 0.5-2.5 hundred million Bacillus halophilus per milliliter, and 0.5-2.5 hundred million Bacillus pumilus per milliliter.
Preferably, the volume ratio of the pseudoxanthomonas, pseudomonas, nitrosomonas, halophilus and pseudomonas suspensions is 2: 2: 1: 1: 1.
in the liquid bacterial fertilizer, the mass contents of nitrogen, phosphorus and potassium are all more than or equal to 4 percent, and the liquid bacterial fertilizer contains the following bacterial components: 1.5-3.5 hundred million Pseudomonas pseudoflava/ml, 0.5-2.5 hundred million Bacillus halophilus/ml, 0.5-2.5 hundred million Bacillus monophila/ml, and 0.5-2.5 hundred million Nitrosomonas.
In the embodiment, the nitrosomonas, pseudomonas, halophilus, pseudomonas and pseudoxanthomonas are all separated from the rhizosphere soil of the sweet potato and can be directly purchased from commercial channels.
Example 2
The preparation method of the embodiment is similar to that of the embodiment 1, and the difference is that the solid bacterial manure is prepared by the following steps:
(21) crushing a base material: crushing the roughly processed crop straws, bran and other base materials into 200-300mm coarse powder serving as an excipient;
(22) mixing materials: mixing the base material with the liquid bacterial manure prepared in the embodiment 1 according to the volume ratio of 1.5-2.0, adsorbing the liquid bacterial manure by using the base material, and simultaneously adding water with corresponding volume to ensure that the temperature of the system reaches 30-40%;
(23) crushing and sieving: uniformly stirring the base material and the liquid bacterial manure, and then further crushing and sieving the mixture, wherein the mesh opening size is 100-200 meshes;
(24) and (3) granulation: and granulating the crushed and sieved materials by a granulator to prepare solid bacterial manure particles with the particle size of 1-2 mm.
In the solid bacterial fertilizer, the mass contents of nitrogen, phosphorus and potassium are all more than or equal to 6 percent, and the solid bacterial fertilizer comprises the following bacterial components: 1.0-2.5 hundred million Pseudomonas pseudoxanthans/gram, 0.5-2.0 hundred million Bacillus halophilus/gram, 0.5-2.0 hundred million Bacillus, and 0.5-2.0 hundred million Nitrosomonas.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The examples are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (8)

1. A method for rapidly and accurately preparing a microbial fertilizer special for sweet potatoes is characterized by comprising the following steps:
(1) sampling sweet potato planting land soil, extracting total DNA of rhizosphere soil bacterial communities from soil samples, performing high-throughput sequencing on a V4-V5 hypervariable region formed by bacterial community DNA16S rRNA genes on an Illumina HiSeq PE250 platform, evaluating 16S rRNA gene composition, and analyzing to obtain the core genus of the sweet potato rhizosphere soil bacterial communities;
(2) and preparing liquid bacterial manure or solid bacterial manure containing the sweet potato rhizosphere soil bacterial community core bacteria according to the analysis result.
2. The method for rapidly and accurately preparing the microbial fertilizer special for sweet potatoes as claimed in claim 1, wherein the preparation method of the liquid microbial fertilizer is as follows: pure strains of each core bacterium of sweet potato rhizosphere soil bacterial community are obtained by adopting a pure culture technology, and liquid bacterial manure is obtained by mixing according to a certain proportion after enlarged culture.
3. The method for rapidly and accurately preparing the microbial fertilizer special for sweet potatoes as claimed in claim 1, wherein the bacterial composition in the liquid bacterial fertilizer comprises 1.5-3.5 hundred million pseudoxanthomonas per milliliter, 1.5-3.5 hundred million pseudomonads per milliliter, 0.5-2.5 hundred million halomyxobacter, 0.5-2.5 hundred million bacillus per milliliter and 0.5-2.5 hundred million nitrosomonas per milliliter.
4. The method for rapidly and accurately preparing the microbial fertilizer special for sweet potatoes according to claim 3, wherein the preparation method of the liquid microbial fertilizer comprises the following steps: under the aseptic condition, respectively inoculating nitrosomonas, pseudomonas, halophilous myxobacter, gemonas and pseudoxanthomonas to an LB culture medium, and culturing at the constant temperature of 28-37 ℃ for 24-36 h; uniformly mixing the cultured bacterial suspension according to a certain volume ratio to ensure that the final concentration is as follows: 1.5-3.5 hundred million Pseudomonas pseudoxanthans per milliliter, 0.5-2.5 hundred million Nitrosomonas nitrosata per milliliter, 0.5-2.5 hundred million Bacillus halophilus per milliliter, and 0.5-2.5 hundred million Bacillus pumilus per milliliter.
5. The method for rapidly and accurately preparing the microbial fertilizer special for sweet potatoes as claimed in claim 1, wherein the preparation method of the solid microbial fertilizer comprises the following steps: pulverizing the base material into coarse powder as excipient; mixing the excipient and the mixed solution of the sweet potato rhizosphere soil bacterial community and each core bacterial strain in the volume ratio of 1.5-2.0: 1, mixing, adsorbing a bacterial solution by using a base material, and adding water with a corresponding volume to enable the temperature to reach 30-40%; and uniformly stirring the base material and the bacterial liquid, further crushing, sieving, granulating and packaging to obtain the microbial inoculum.
6. The method for rapidly and accurately preparing the microbial fertilizer special for sweet potatoes as claimed in claim 5, wherein the base material is roughly processed crop straws or bran.
7. The method for rapidly and accurately preparing the microbial fertilizer special for sweet potatoes as claimed in claim 1, wherein the solid bacterial fertilizer comprises the following bacteria: 1.0-2.5 hundred million Pseudomonas pseudoxanthans/gram, 0.5-2.0 hundred million Bacillus halophilus/gram, 0.5-2.0 hundred million Bacillus, and 0.5-2.0 hundred million Nitrosomonas.
8. The microbial fertilizer special for sweet potatoes, which is prepared by the method of any one of claims 1 to 7.
CN202010557874.2A 2020-06-18 2020-06-18 Method for rapidly and accurately preparing microbial fertilizer special for sweet potatoes and prepared microbial fertilizer Pending CN111676161A (en)

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