CN111635852A - Respiratory tract pathogenic microorganism collection system - Google Patents
Respiratory tract pathogenic microorganism collection system Download PDFInfo
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- CN111635852A CN111635852A CN202010512752.1A CN202010512752A CN111635852A CN 111635852 A CN111635852 A CN 111635852A CN 202010512752 A CN202010512752 A CN 202010512752A CN 111635852 A CN111635852 A CN 111635852A
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- 244000000010 microbial pathogen Species 0.000 title claims abstract description 63
- 210000002345 respiratory system Anatomy 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 85
- 239000002699 waste material Substances 0.000 claims abstract description 63
- 238000005336 cracking Methods 0.000 claims abstract description 36
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 30
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 30
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 30
- 230000009089 cytolysis Effects 0.000 claims abstract description 26
- 238000007599 discharging Methods 0.000 claims abstract description 9
- 238000007664 blowing Methods 0.000 claims description 31
- 239000006166 lysate Substances 0.000 claims description 22
- 239000012528 membrane Substances 0.000 claims description 14
- 238000000108 ultra-filtration Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 8
- 210000000214 mouth Anatomy 0.000 claims description 5
- 208000000059 Dyspnea Diseases 0.000 claims description 4
- 206010013975 Dyspnoeas Diseases 0.000 claims description 4
- 244000052769 pathogen Species 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000007689 inspection Methods 0.000 abstract description 5
- 239000007789 gas Substances 0.000 description 34
- 208000015181 infectious disease Diseases 0.000 description 8
- 238000007789 sealing Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a respiratory tract pathogenic microorganism collecting device, which comprises a collecting pipe for collecting pathogenic microorganisms, a cracking area for storing cracking liquid to crack the pathogenic microorganisms, a guide pipe, an enrichment area for enriching cracked nucleic acid molecules and a waste liquid area for storing waste liquid and discharging gas, wherein the collecting pipe is connected with the cracking area, the cracking area is connected with an inlet of the enrichment area through the guide pipe, and an outlet of the enrichment area is connected with the waste liquid area; when the collecting pipe collects pathogenic microorganisms, the guide pipe is positioned above the liquid level of the lysis solution, and after the lysis zone finishes the lysis, the guide pipe is inserted below the liquid level of the lysis solution; the waste liquid area is provided with an air outlet pipe which is used for exhausting gas and communicated with the outside atmosphere. Therefore, the collection position is not limited by a swab collection mode, more pathogens can be collected, the pathogens in the enrichment area are relatively enriched, and the detection result is relatively stable; the operation is simple, and the auxiliary collection operation of medical personnel or inspection personnel is not needed.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a respiratory tract pathogenic microorganism collecting device.
Background
In the prior art, respiratory pathogenic microorganisms are collected by various swabs such as throat swabs, nose swabs, oral swabs and the like.
Because the swab can only collect the scraped part of the swab, the quantity of the collected pathogenic microorganisms is limited, the pathogenic microorganisms are not enriched enough, and the sampling result is unstable. In addition, when medical staff or inspection personnel carry out the operation of gathering to the person of being gathered, there is certain infection risk.
In summary, how to increase the number of collected pathogenic microorganisms on the premise of reducing the risk of infection is a problem to be urgently solved by those skilled in the art.
Disclosure of Invention
In view of this, the present invention provides a respiratory tract pathogenic microorganism collecting device, which can collect and capture pathogenic microorganisms in exhaled air through a capturing area, and the collected pathogenic microorganisms are collected in a large quantity, and can be operated by a collector, thereby reducing the risk of infection.
In order to achieve the above purpose, the invention provides the following technical scheme:
a respiratory tract pathogenic microorganism collecting device comprises a collecting pipe for collecting pathogenic microorganisms, a cracking area for storing lysate to crack the pathogenic microorganisms, a guide pipe, an enrichment area for filtering and enriching cracked nucleic acid molecules and a waste liquid area for storing waste liquid and discharging gas, wherein the collecting pipe is hermetically connected with the cracking area, the cracking area is hermetically connected with an inlet of the enrichment area through the guide pipe, and an outlet of the enrichment area is hermetically connected with the waste liquid area;
when the collecting pipe collects the pathogenic microorganisms, the guide pipe is positioned above the liquid level of the lysis solution, and after the lysis zone finishes the lysis, the guide pipe is inserted below the liquid level of the lysis solution;
and the waste liquid area is provided with an air outlet pipe used for exhausting gas and communicated with the external atmosphere.
Preferably, the collecting pipe comprises an air inlet pipe communicated with the cracking area and an air blowing port for collecting exhaled air, and the air blowing port is connected with the air inlet pipe;
the air inlet pipe is inserted below the liquid level of the lysate.
Preferably, the air blowing opening is a thin tube for inserting into the oral cavity of the subject.
Preferably, the blowing opening is a blowing nozzle which is used for being tightly attached to the face of the person to be collected.
Preferably, the collecting pipe is connected with an air exhaust device so as to assist in completing collection of pathogenic microorganisms of the patient with dyspnea in an air exhaust mode.
Preferably, the enrichment district is the infundibulate, the enrichment district is including being used for filtering the ultrafiltration membrane of nucleic acid molecule and being used for collecting the nucleic acid molecule, be located the collection room of funnel neck department, the waste liquid district cup joint in outside the enrichment district, in order to deposit through the ultrafiltration membrane the waste liquid.
Preferably, the waste liquid area comprises a waste liquid bottle for containing the waste liquid, and the waste liquid bottle is communicated with the air outlet pipe.
Preferably, at least one filter membrane for intercepting the waste liquid and the pathogenic microorganisms is arranged at the gas outlet pipe so as to prevent the discharged gas from polluting the external environment.
The invention provides a respiratory tract pathogenic microorganism collecting device, which comprises a collecting pipe for collecting pathogenic microorganisms, a cracking area for storing cracking liquid to crack the pathogenic microorganisms, a guide pipe, an enrichment area for enriching cracked nucleic acid molecules and a waste liquid area for storing waste liquid and discharging gas, wherein the guide pipe is used for storing the nucleic acid molecules; the collecting pipe is hermetically connected with the cracking zone, the cracking zone is hermetically connected with the inlet of the enrichment zone through a guide pipe, and the outlet of the enrichment zone is hermetically connected with the waste liquid zone; when the collecting pipe collects pathogenic microorganisms, the guide pipe is positioned above the liquid level of the lysis solution, and after the lysis zone finishes the lysis, the guide pipe is inserted below the liquid level of the lysis solution; the waste liquid area is provided with an air outlet pipe for discharging gas.
When the device is used, a collected person blows air into the collecting pipe continuously, the air exhaled by the collected person enters the cracking zone through the collecting pipe, and pathogenic microorganisms are cracked and inactivated by the cracking liquid and release nucleic acid molecules; inserting the conduit below the liquid level of the lysate, and continuously blowing air into the collection tube by the collected person or forcing the lysate and the nucleic acid molecules dissolved in the lysate to be pressed into the conduit and enter the enrichment region through the conduit in a way of ventilating and pressurizing the lysis region; the enrichment area filters and enriches the cracked nucleic acid molecules and discharges the rest waste liquid into the waste liquid area.
Preferably, the collecting tube can be connected with an air suction device so as to assist the collection of pathogenic microorganisms of the patient with dyspnea by making a negative pressure air suction.
Therefore, the collection device provided by the invention is not limited by the collection position in the swab collection mode in the prior art, more pathogens can be collected, and the pathogens in the enrichment area are relatively enriched, so that the detection result is relatively stable. Meanwhile, the operation is simple, medical staff or inspection staff are not needed for assisting in collecting operation, and the waste liquid generated by the cracking microorganisms is reserved in the waste liquid area, so that the infection risk is greatly reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a specific embodiment of a respiratory tract pathogenic microorganism collecting device provided by the invention at a pathogenic microorganism collecting stage;
FIG. 2 is a schematic diagram of the respiratory pathogenic microorganism collecting device in FIG. 1 at the nucleic acid molecule delivery stage.
In fig. 1-2:
1 is a collecting pipe, 11 is a gas blowing port, 12 is a gas inlet pipe, 2 is a cracking area, 3 is a guide pipe, 4 is an enrichment area, 41 is an ultrafiltration membrane, 5 is a waste liquid area, 51 is a gas outlet pipe, 6 is lysate and 7 is waste liquid.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The core of the invention is to provide a respiratory tract pathogenic microorganism collecting device, which can collect and capture pathogenic microorganisms in exhaled air through a capturing area, the collection quantity is large, and the collected person can operate the collecting device by himself, thereby reducing the infection risk.
Referring to fig. 1-2, fig. 1 is a schematic structural diagram of an embodiment of a respiratory tract pathogenic microorganism collecting device provided by the present invention; FIG. 2 is a schematic diagram of the respiratory pathogenic microorganism collecting device in FIG. 1 at the nucleic acid molecule delivery stage.
The invention provides a respiratory tract pathogenic microorganism collecting device, which comprises a collecting pipe 1 for collecting pathogenic microorganisms, a cracking zone 2 for storing a cracking liquid 6 to crack the pathogenic microorganisms, a conduit 3, an enrichment zone 4 for enriching cracked nucleic acid molecules and a waste liquid zone 5 for storing waste liquid 7 and discharging gas, wherein the collecting pipe 1 is hermetically connected with the cracking zone 2, the cracking zone 2 is hermetically connected with an inlet of the enrichment zone 4 through the conduit 3, and an outlet of the enrichment zone 4 is hermetically connected with the waste liquid zone 5; when the collecting pipe 1 collects pathogenic microorganisms, the conduit 3 is positioned above the liquid level of the lysate 6, and after the cracking zone 2 finishes cracking, the conduit 3 is inserted below the liquid level of the lysate 6; the waste liquid region 5 is provided with an outlet pipe 51 for discharging gas, communicating with the outside atmosphere.
The collecting pipe 1 is used for collecting the gas exhaled by the person to be collected and guiding the gas into the cracking zone 2, preferably, referring to fig. 1, the collecting pipe 1 may include a gas inlet pipe 12 communicated with the cracking zone 2 and a gas blowing port 11 used for collecting the exhaled gas, and the gas blowing port 11 is connected with the gas inlet pipe 12; the air inlet tube 12 is inserted below the liquid level of the lysate 6.
When pathogenic microorganisms are collected, gas exhaled by a collected person sequentially passes through the gas blowing port 11 and the gas inlet pipe 12 and is introduced into the lysate 6, and the pathogenic microorganisms in the exhaled gas are cracked by the lysate 6 and release nucleic acid molecules; after completion of the lysis, the tube 3 is inserted below the liquid level of the lysis solution 6, so that the lysis solution 6 and the nucleic acid molecules therein in the lysis zone 2 are forced into the tube 3 by the gas pressure when the gas pressure in the lysis zone 2 is increased.
The air inlet pipe 12 is inserted below the liquid level of the lysate 6 to facilitate the reaction of pathogenic microorganisms in the exhaled air with the lysate 6, and certainly, the air inlet pipe can also be arranged above the liquid level of the lysate 6, but the reaction time of the pathogenic microorganisms with the lysate 6 should be properly prolonged to meet the requirement of cracking the pathogenic microorganisms.
The blowing port 11 is used for collecting the gas exhaled by the person to be collected, and preferably, the blowing port 11 may be a thin tube for inserting into the oral cavity of the person to be collected. When collecting gas, the gas blowing port 11 is placed in the oral cavity of the person to be collected and then blown, so that the gas can be introduced into the cracking zone 2 through the gas blowing port 11 and the gas inlet pipe 12.
Considering that the air blowing port 11 needs to be placed in the oral cavity of the subject, the air blowing port 11 should be a sterilized sterile tube to avoid affecting the health of the subject.
Preferably, referring to fig. 1, the blowing port 11 may be a blowing nozzle for closely fitting the face of the person to be collected.
Because the air blowing port 11 is attached to the face of the collected person, all the air exhaled by the collected person is guided into the cracking zone 2 through the air blowing port 11 and the air inlet pipe 12, the air containing pathogenic microorganisms cannot directly enter the external environment, and the infection risk is reduced.
The specific shape of the blowing nozzle can refer to the blowing nozzle used for detecting the vital capacity in the prior art, and can be designed into other shapes as long as the requirement of tight fit with the face of a human body is met.
Preferably, the air blowing port 11 can be made of sterilized plastic and is disposable, so that the infection risk is reduced. Of course, the air blowing port 11 can also be made of high-temperature-resistant sterilization materials, and the air blowing port 11 can be recycled through high-temperature sterilization, so that the inspection material cost is saved.
The cracking zone 2 is used for cracking pathogenic microorganisms and obtaining nucleic acid molecules, and can be formed by common biological experimental equipment such as a beaker and a flask, and the like as long as the requirement of sealing connection at the connection part of the air inlet pipe 12 and the conduit 3 is met.
The components and the ratio of the lysis solution 6 are referred to the prior art and are not described herein. The amount of the lysis solution 6 in the lysis zone 2 should be determined according to the volume of the container and the number of nucleic acid molecules to be collected in the actual experiment, so as to reduce the waste of the lysis solution 6 as much as possible while ensuring that enough pathogenic nucleic acid molecules are obtained, and save the collection cost.
The enrichment region 4 is used for filtering the mixed liquid of the expired gas entering from the conduit 3, the lysate 6 and the nucleic acid molecules to enrich the nucleic acid molecules and discharging the residual waste liquid 7 and the waste gas into the waste liquid region 5.
Preferably, referring to fig. 1, the enrichment region 4 may be configured in a funnel shape, the enrichment region 4 includes an ultrafiltration membrane 41 for filtering nucleic acid molecules and a collection chamber at a funnel neck for collecting nucleic acid molecules, and the waste liquid region 5 is sleeved outside the enrichment region 4 to store the waste liquid 7 passing through the ultrafiltration membrane 41.
In order to prevent gases included in the waste liquid 7 from being discharged from a gap between the enrichment region 4 and the waste liquid region 5, the waste liquid region 5 and the enrichment region 4 are in sealing connection, the sealing connection can be as shown in figure 1, a frosted part is arranged on the outer wall surface of the enrichment region 4, a frosted part is arranged on the inner wall surface of the upper part of the waste liquid region 5, and the frosted part are sleeved; or sealing elements such as sealing rings and the like can be arranged at the joint of the enrichment area 4 and the waste liquid area 5; of course, other sealing means may be used to seal the junction of the enrichment zone 4 and the reject zone 5.
The material of the ultrafiltration membrane 41 and the filtration precision of the ultrafiltration membrane 41 should be selected according to the size of the nucleic acid molecules of the pathogenic microorganisms of the respiratory tract in the actual experiment by referring to the prior art.
Preferably, in order to conveniently take out the nucleic acid molecules enriched in the collection chamber for detection, the ultrafiltration membrane 41 can be detachably connected with the collection chamber, so that the collection chamber can be directly taken down after the enrichment region 4 is taken out after collection is completed, and the nucleic acid molecules in the collection chamber can be conveniently detected by matching with a downstream direct amplification reagent.
When the device is used, a person to be collected blows air into the collecting pipe 1 continuously, the air exhaled by the person to be collected enters the cracking zone 2 through the collecting pipe 1, and pathogenic microorganisms are cracked and inactivated by the cracking liquid 6 and release nucleic acid molecules; inserting the conduit 3 below the liquid level of the lysate 6, and continuously blowing air into the collecting pipe 1 by the collected person or forcing the lysate 6 and the nucleic acid molecules dissolved in the lysate 6 to be pressed into the conduit 3 in a way of ventilating and pressurizing the lysis zone 2 and enter the enrichment zone 4 through the conduit 3; the enrichment area 4 filters and enriches the cracked nucleic acid molecules and discharges the rest of the waste liquid 7 into the waste liquid area 5.
Preferably, the collection tube 1 can be connected to a suction device to assist the collection of pathogenic microorganisms of dyspnea patients by creating a negative pressure inhalation.
The air extracting device may be an air extracting pump, an air extracting cylinder, etc., and the specific structure thereof should be selected according to the needs in actual production by referring to the prior art, which is not described herein again.
In this embodiment, the collecting device is not limited by the collecting position, and can collect more pathogens, and the pathogens in the capturing region 2 are relatively enriched, so the detection result is relatively stable.
Meanwhile, the operation is simple, the auxiliary collection operation of medical staff or inspection personnel is not needed, and the waste liquid 7 generated by the cracking microorganisms is reserved in the waste liquid area 5, so that the infection risk is greatly reduced.
On the basis of the above embodiment, the waste liquid area 5 can be set to include a waste liquid bottle for containing the waste liquid 7, the waste liquid bottle is communicated with the air outlet pipe 51, and the gas mixed in the waste liquid 7 can be discharged through the air outlet pipe 51.
Preferably, the waste liquid bottle and the air outlet pipe 51 may be integrated, and the waste liquid bottle and the air outlet pipe may be directly used in a conical bottle with a branch pipe, a flask with a branch pipe, or the like.
Of course, the waste zone 5 can also be assembled using a jar and catheter sealed connection.
Preferably, at least one filter membrane for intercepting the waste liquid 7 and pathogenic microorganisms is disposed at the outlet pipe 51 to prevent the discharged gas from polluting the external environment. The types and the filtration precision of the filter membranes are selected according to the sizes of pathogenic microorganisms and the like by referring to the prior art.
The respiratory tract pathogenic microorganism collecting device provided by the invention is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (8)
1. The respiratory tract pathogenic microorganism collecting device is characterized by comprising a collecting pipe (1) for collecting pathogenic microorganisms, a cracking area (2) for storing a lysis solution (6) to crack the pathogenic microorganisms, a guide pipe (3), an enrichment area (4) for filtering and enriching cracked nucleic acid molecules and a waste liquid area (5) for storing waste liquid (7) and discharging gas, wherein the collecting pipe (1) is hermetically connected with the cracking area (2), the cracking area (2) is hermetically connected with an inlet of the enrichment area (4) through the guide pipe (3), and an outlet of the enrichment area (4) is hermetically connected with the waste liquid area (5);
when the collection pipe (1) collects the pathogenic microorganisms, the conduit (3) is positioned above the liquid level of the lysate (6), and after the lysis of the lysis zone (2) is completed, the conduit (3) is inserted below the liquid level of the lysate (6);
the waste liquid area (5) is provided with an air outlet pipe (51) which is used for discharging gas and communicated with the outside atmosphere.
2. The respiratory tract pathogenic microorganism collecting device according to claim 1, wherein the collecting pipe (1) comprises an air inlet pipe (12) communicated with the cracking zone (2) and an air blowing port (11) for collecting exhaled air, and the air blowing port (11) is connected with the air inlet pipe (12);
the air inlet pipe (12) is inserted below the liquid level of the lysate (6).
3. The device for collecting pathogenic microorganisms of respiratory tract according to claim 2, wherein the air blowing port (11) is a thin tube for inserting into the oral cavity of the person to be collected.
4. The respiratory tract pathogenic microorganism collecting device according to claim 2, wherein the air blowing opening (11) is a blowing nozzle for closely fitting with the face of the person to be collected.
5. The respiratory tract pathogenic microorganism collecting device according to claim 2, wherein the collecting pipe (1) is connected with an air suction device to assist in collecting pathogenic microorganisms of patients with dyspnea by means of air suction.
6. The collection device of pathogenic microorganisms of respiratory tract according to claim 1, wherein the enrichment region (4) is funnel-shaped, the enrichment region (4) comprises an ultrafiltration membrane (41) for filtering the nucleic acid molecules and a collection chamber for collecting the nucleic acid molecules and located at the funnel neck, and the waste liquid region (5) is sleeved outside the enrichment region (4) to store the waste liquid (7) passing through the ultrafiltration membrane (41).
7. The apparatus for collecting respiratory tract pathogenic microorganisms according to any one of claims 1 to 6, wherein the waste liquid area (5) comprises a waste liquid bottle for containing the waste liquid (7), and the waste liquid bottle is communicated with the air outlet pipe (51).
8. The device for collecting pathogenic microorganisms of respiratory tract according to claim 7, wherein at least one filter membrane for intercepting the waste liquid (7) and the pathogenic microorganisms is arranged at the gas outlet pipe (51) so as to prevent the discharged gas from polluting the external environment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010512752.1A CN111635852A (en) | 2020-06-08 | 2020-06-08 | Respiratory tract pathogenic microorganism collection system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010512752.1A CN111635852A (en) | 2020-06-08 | 2020-06-08 | Respiratory tract pathogenic microorganism collection system |
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| CN111635852A true CN111635852A (en) | 2020-09-08 |
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| CN202010512752.1A Pending CN111635852A (en) | 2020-06-08 | 2020-06-08 | Respiratory tract pathogenic microorganism collection system |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201527375U (en) * | 2009-06-16 | 2010-07-14 | 上海捷瑞生物工程有限公司 | Nucleic acid separating and purifying column |
| US20110250680A1 (en) * | 2008-12-10 | 2011-10-13 | Biomerieux | Automated system for the lysis of microorganisms present in a sample, for extraction and for purification of the nucleic acids of said microorganisms for purposes of analysis |
| CN103119417A (en) * | 2010-07-06 | 2013-05-22 | 戴顿公司 | System for airborne bacterial sample collection and analysis |
| CN205133629U (en) * | 2015-08-28 | 2016-04-06 | 沈阳医学院附属中心医院 | A purifying column for body fluid trace nucleic acid extraction |
| CN106085830A (en) * | 2016-08-30 | 2016-11-09 | 浙江省疾病预防控制中心 | Pathogenic microorganism collection device in air |
| CN106754313A (en) * | 2017-01-12 | 2017-05-31 | 武汉菲思特生物科技有限公司 | Nucleic acid sequencing apparatus and system |
| CN111172239A (en) * | 2020-02-28 | 2020-05-19 | 上海思路迪医学检验所有限公司 | Virus sample preserving fluid, nucleic acid extraction reagent and virus nucleic acid extraction method |
| CN111195139A (en) * | 2020-03-06 | 2020-05-26 | 葛虎 | Respiratory tract virus respiratory type collecting tube |
-
2020
- 2020-06-08 CN CN202010512752.1A patent/CN111635852A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110250680A1 (en) * | 2008-12-10 | 2011-10-13 | Biomerieux | Automated system for the lysis of microorganisms present in a sample, for extraction and for purification of the nucleic acids of said microorganisms for purposes of analysis |
| CN201527375U (en) * | 2009-06-16 | 2010-07-14 | 上海捷瑞生物工程有限公司 | Nucleic acid separating and purifying column |
| CN103119417A (en) * | 2010-07-06 | 2013-05-22 | 戴顿公司 | System for airborne bacterial sample collection and analysis |
| CN205133629U (en) * | 2015-08-28 | 2016-04-06 | 沈阳医学院附属中心医院 | A purifying column for body fluid trace nucleic acid extraction |
| CN106085830A (en) * | 2016-08-30 | 2016-11-09 | 浙江省疾病预防控制中心 | Pathogenic microorganism collection device in air |
| CN106754313A (en) * | 2017-01-12 | 2017-05-31 | 武汉菲思特生物科技有限公司 | Nucleic acid sequencing apparatus and system |
| CN111172239A (en) * | 2020-02-28 | 2020-05-19 | 上海思路迪医学检验所有限公司 | Virus sample preserving fluid, nucleic acid extraction reagent and virus nucleic acid extraction method |
| CN111195139A (en) * | 2020-03-06 | 2020-05-26 | 葛虎 | Respiratory tract virus respiratory type collecting tube |
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Application publication date: 20200908 |