CN201527375U - Nucleic acid separating and purifying column - Google Patents
Nucleic acid separating and purifying column Download PDFInfo
- Publication number
- CN201527375U CN201527375U CN2009200763500U CN200920076350U CN201527375U CN 201527375 U CN201527375 U CN 201527375U CN 2009200763500 U CN2009200763500 U CN 2009200763500U CN 200920076350 U CN200920076350 U CN 200920076350U CN 201527375 U CN201527375 U CN 201527375U
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- China
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- nucleic acid
- column
- adsorption column
- separate nucleic
- absorbing
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Abstract
The utility model relates to a nucleic acid separating and purifying column, which comprises an absorbing column and a waste liquid collecting pipe. The absorbing column is sleeved inside the waste liquid collecting pipe, an absorbing layer is placed on the bottom portion of the absorbing column, and a fastener for fastening the absorbing layer is arranged inside the absorbing column. The nucleic acid separating and purifying column fundamentally resolves difficult problems that nucleic acid absorbing materials are easy to be loosened during a nucleic acid separating and purifying process, and high-efficiently separates and purifies various DNA and RNA large biological molecules, can high-efficiently fix the nucleic acid absorbing materials, and then greatly increases application prospect of the absorbing column type nucleic acid purifying technique. Moreover, by aid of the waste liquid collecting pipe, the problem of treatment of waste substances during the nucleic acid separating and purifying process is resolved in a humanized form.
Description
Technical field
The utility model separate nucleic acid purification column relates to a kind of separate nucleic acid purification devices.
Background technology
The life science technical field relates to macromolecular separation and purification operation such as RNA (ribonucleic acid) DNA, DNA (deoxyribonucleic acid) RNA, and high efficiency separation and purification nucleic acid molecule becomes the gordian technique in this field.But in separate nucleic acid purification process process, the nucleic acid sorbing material very easily comes off.
Summary of the invention
The purpose of this utility model is to provide a kind of separate nucleic acid purification column that can efficiently finish the requirement of separate nucleic acid purifying.
For achieving the above object, the utility model provides a kind of separate nucleic acid purification column (GenClean Column), to satisfy the macromolecular basic demand of this field separation and purification biological nucleic acid, this purification column comprises adsorption column, waste collection pipe, adsorption column is enclosed within the waste collection pipe, adsorbed layer places the adsorption column bottom, wherein, is provided with the buckle that fixes adsorbed layer in adsorption column.
For effective fixedly adsorbed layer, buckle fixes annulus by annular flange flange that is located at adsorption column inwall bottom or groove and constitutes, and buckle is located at the adsorbed layer upper surface, wherein, annulus is embedded in the annular groove or is stuck under the annular flange flange, and annulus is a plastic circle ring, and its internal diameter is less than the external diameter of adsorbed layer.In order to seal the separate nucleic acid purification column, the adsorption column top is connected with the cylindrical plag capping of a sealing adsorption column.
In order there not to be waste liquid to overflow the waste collection pipe in purge process, there are protruding rank the adsorption column upper end, with the upper shed coupling of waste collection pipe.
The invention has the advantages that:
But the various DNA of 1 high efficiency separation purifying, RNA biomacromolecule;
2, unique " buckle-type " technology, efficiently the fixed nucleic acid sorbing material has fundamentally solved the chronic disease that separate nucleic acid purification process process amplifying nucleic acid sorbing material very easily comes off, and has promoted absorption pillar nucleic acid purification The Application of Technology prospect greatly;
3, the waste collection pipe makes the handling problem of waste material in the separate nucleic acid purge process obtain the solution of hommization.
Description of drawings
Fig. 1 is the front view of the utility model separate nucleic acid purification column waste collection pipe.
Fig. 2 is the front view of the utility model separate nucleic acid purification column adsorption column.
Fig. 3 is the front view of the bright annulus of the utility model separate nucleic acid purification column.
Fig. 4 is the vertical view of the bright annulus of the utility model separate nucleic acid purification column.
Fig. 5 is the unitized construction front view of the utility model separate nucleic acid purification column.
Label declaration in the accompanying drawing
1-waste collection pipe 2-adsorption column
21-cylindrical plag capping 22-flange
The protruding rank of 23-3-annulus
The 4-adsorbed layer
Embodiment
See also the front view of Fig. 1 the utility model separate nucleic acid purification column waste collection pipe, the die sinking tool is made the waste collection pipe 1 of plastic material, and waste collection pipe 1 is a circular arc for the bottom, upper end open.
See also the front view of Fig. 2 the utility model separate nucleic acid purification column adsorption column, the die sinking tool is made the adsorption column 2 of plastic material, and this adsorption column 2 is removed the height of cylindrical plag capping 21 length overalls less than waste collection pipe 1 inwall.Adsorption column 2 lower ending openings draw in, play the effect of replacing sorbing material, and place, the nearly bottom of adsorption column 2 inwalls is provided with annular flange flange 22, and there are protruding rank 23 upper end.
See also the front view of annulus in Fig. 3 the utility model separate nucleic acid purification column and the vertical view of Fig. 4 annulus, the external diameter of annulus 3 is consistent with adsorption column 2 internal diameters.
See also the unitized construction front view of Fig. 5 the utility model separate nucleic acid purification column, the present embodiment purification column comprises adsorption column 2, waste collection pipe 1, adsorption column 2 is enclosed within the waste collection pipe 1, the adsorbed layer 4 that a certain amount of accounting sorbing material is made places adsorption column 2 bottoms to draw on the mouth, wherein, in adsorption column 2, be provided with the buckle that fixes adsorbed layer 4.Annulus 3 places on the adsorbed layer 4, and the external diameter of this adsorbed layer 4 is greater than the internal diameter of annulus 3, and adsorbed layer 4 need guarantee that with flange 22 spacings annulus 3 can be stuck in annular flange flange 22 times, and adsorbed layer 4 is fixed in the adsorption column 2.The cylindrical plag capping 21 that is connected with on the adsorption column 2 is filled in and is made its sealing in the adsorption column 2.The protruding rank 23 of adsorption column 2 upper ends are enclosed within the upper shed sealing of waste collection pipe 1.
Claims (6)
1. a separate nucleic acid purification column comprises adsorption column, waste collection pipe, and adsorption column is enclosed within the waste collection pipe, and adsorbed layer places the adsorption column bottom, it is characterized in that: be provided with the buckle that is used to fix adsorbed layer in adsorption column.
2. separate nucleic acid purification column according to claim 1 is characterized in that described buckle is made of the annular flange flange or the groove fixing annulus of the bottom that is located at the adsorption column inwall.
3. separate nucleic acid purification column according to claim 1 and 2 is characterized in that: described buckle is located at the adsorbed layer upper surface, and wherein, annulus is embedded in the annular groove or is stuck under the annular flange flange.
4. separate nucleic acid purification column according to claim 2 is characterized in that: described annulus is a plastic circle ring, and its internal diameter is less than the external diameter of adsorbed layer.
5. separate nucleic acid purification column according to claim 1 is characterized in that: the adsorption column top is connected with the cylindrical plag capping of a sealing adsorption column.
6. separate nucleic acid purification column according to claim 5 is characterized in that: there are protruding rank the adsorption column upper end, with the upper shed coupling of waste collection pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200763500U CN201527375U (en) | 2009-06-16 | 2009-06-16 | Nucleic acid separating and purifying column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200763500U CN201527375U (en) | 2009-06-16 | 2009-06-16 | Nucleic acid separating and purifying column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201527375U true CN201527375U (en) | 2010-07-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009200763500U Expired - Fee Related CN201527375U (en) | 2009-06-16 | 2009-06-16 | Nucleic acid separating and purifying column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201527375U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105414963A (en) * | 2015-12-29 | 2016-03-23 | 上海伦恩通用技术有限公司 | Full-automatic assembling equipment for adsorption trap |
| CN108192813A (en) * | 2018-04-09 | 2018-06-22 | 杭州倍强医药科技有限公司 | A kind of trace dna elutes collection device |
| CN108728432A (en) * | 2018-06-08 | 2018-11-02 | 浙江大学 | The method and its application of efficient purified virus double-stranded RNA genome in plant tissue |
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | The fixing pipe and nucleic acid extraction component of nucleic acid extraction component |
| CN110201934A (en) * | 2019-06-12 | 2019-09-06 | 江苏睿玻生物科技有限公司 | Negative pressure cleaning device and negative pressure cleaning method |
| CN111635852A (en) * | 2020-06-08 | 2020-09-08 | 苏州新海生物科技股份有限公司 | Respiratory tract pathogenic microorganism collection system |
| WO2021258714A1 (en) * | 2020-06-23 | 2021-12-30 | 广州洁特生物过滤股份有限公司 | Nucleic acid purification column |
-
2009
- 2009-06-16 CN CN2009200763500U patent/CN201527375U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105414963A (en) * | 2015-12-29 | 2016-03-23 | 上海伦恩通用技术有限公司 | Full-automatic assembling equipment for adsorption trap |
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | The fixing pipe and nucleic acid extraction component of nucleic acid extraction component |
| CN108192813A (en) * | 2018-04-09 | 2018-06-22 | 杭州倍强医药科技有限公司 | A kind of trace dna elutes collection device |
| CN108728432A (en) * | 2018-06-08 | 2018-11-02 | 浙江大学 | The method and its application of efficient purified virus double-stranded RNA genome in plant tissue |
| CN110201934A (en) * | 2019-06-12 | 2019-09-06 | 江苏睿玻生物科技有限公司 | Negative pressure cleaning device and negative pressure cleaning method |
| CN110201934B (en) * | 2019-06-12 | 2023-09-05 | 江苏睿玻生物科技有限公司 | Negative pressure cleaning device and negative pressure cleaning method |
| CN111635852A (en) * | 2020-06-08 | 2020-09-08 | 苏州新海生物科技股份有限公司 | Respiratory tract pathogenic microorganism collection system |
| WO2021258714A1 (en) * | 2020-06-23 | 2021-12-30 | 广州洁特生物过滤股份有限公司 | Nucleic acid purification column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100714 Termination date: 20150616 |
|
| EXPY | Termination of patent right or utility model |