CN214496307U - Collection and enrichment system of microorganism aerosol - Google Patents

Abstract

The utility model provides a collection and enrichment system of microorganism aerosol, collect subassembly, aerosol enrichment subassembly and sample collection subassembly including aerosol, the input at the aerosol enrichment subassembly is connected to the aerosol collection subassembly, and the sample collection subassembly cover is established in the aerosol enrichment subassembly outside. The aerosol enrichment assembly comprises an aerosol enrichment device and a collecting pipe cover, wherein cell maintenance liquid is contained in the aerosol enrichment device, the bottom end of the aerosol enrichment device is open, and a filter plate is packaged outside the open; gather and wear to be equipped with into trachea and outlet duct on the pipe cover, it includes first end and second end to go into the trachea, the outlet duct includes third end and fourth end, first end and third end are located the top of gathering the pipe cover, second end and fourth all are located aerosol enrichment ware, the porous spheroid of second end fixedly connected with, the porous spheroid is located cell maintenance liquid, first end is connected with an aspiration pump, the fourth end is located the liquid level top of cell maintenance liquid, the detachably cartridge has the stopper in the third end.

Description

Collection and enrichment system of microorganism aerosol

Technical Field

The utility model relates to the technical field of medical supplies, in particular to collection and enrichment system of microorganism aerosol.

Background

The novel coronavirus pneumonia (Corona Virus Disease 2019, COVID-19) is abbreviated as 'novel coronavirus pneumonia', and is pneumonia caused by 2019 novel coronavirus infection, respiratory tract infection is taken as a main transmission path of the Virus, the Virus spread through aerosol is easy to cause large-scale infection of people in a short time, and the transmission speed is very high. Therefore, virus monitoring research on aerosol in the environment is necessary, but as the traditional bioaerosol collecting instrument or device mouth-to-mouth detection method is mainly applicable to bacteria and fungi pathogens, the monitoring of the virus aerosol is limited, the operation of monitoring the virus is inconvenient, the sensitivity is poor, the current monitoring research situation in the current infectious disease field is seriously not adapted, the pathogen monitoring requirement in the environment is restricted, and a novel virus sampler becomes one of the current research hotspots in the field.

The virus is a non-cellular organism which is small in size, only contains one kind of nucleic acid, must be counted in living cells and proliferated in a replication mode, is small in particle size and is not easy to collect respiratory viruses in ambient air. The viral air collector should focus on aerosol particles produced by breathing, and most of the existing biological aerosol samplers are not specifically set for viral aerosols, have wide collection spectrum and are not suitable for collecting virus samples.

At present, a virus aerosol collector is used for collecting pathogenic microorganisms in the process of collecting the pathogenic microorganisms, a vacuum air pump is used for pumping gas containing aerosol into a sample collecting pipe, but the gas and the liquid cannot be effectively mixed in the collecting process, so that the success rate of virus culture and detection is low. In addition, the virus is directly detected only through the liquid after the aerosol enrichment, and the virus is influenced by large particles such as bacteria, so that the detection effect is not high, and the culture and research of the bacteria accompanied by the aerosol are not convenient. Meanwhile, when the aerosol is collected, the ambient gas is generally directly extracted, and the density of the aerosol in the ambient gas is low, so that the aerosol collection efficiency is not high.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing the device.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a collection and enrichment system of microorganism aerosol, collects subassembly, aerosol enrichment subassembly and sample collection subassembly including the aerosol, the aerosol is collected the subassembly and is connected the input of aerosol enrichment subassembly, the sample collection subassembly cover is established the aerosol enrichment subassembly outside.

The aerosol enrichment assembly comprises an aerosol enrichment device and a collection pipe cover, the collection pipe cover is mounted on the upper port of the aerosol enrichment device, cell maintenance liquid is contained in the aerosol enrichment device, the bottom end of the aerosol enrichment device is open, and a filter plate is packaged outside the open end; gather and wear to be equipped with into trachea and outlet duct on the pipe cover, it includes relative first end and second end to go into the trachea, the outlet duct includes relative third end and fourth end, first end with the third end is located gather the top of pipe cover, the second end with the fourth end all is located in the aerosol enrichment ware, the porous spheroid of second end fixedly connected with, the porous spheroid is located in the cell maintenance liquid, first end is connected with first aspiration pump, the fourth end is located the liquid level top of cell maintenance liquid, detachably cartridge has the stopper in the third end.

Preferably, a plurality of circular holes are uniformly distributed on the porous spheroid, the circular holes penetrate through the porous spheroid and are communicated with the air inlet pipe, and the circular holes are in contact with the cell maintenance liquid.

Preferably, a filtering component is arranged in the air outlet pipe, the filtering component sequentially comprises a first filtering layer and a second filtering layer from bottom to top, the first filtering layer is located below the pipe cover, and the second filtering layer is located above the sample pipe cover.

Preferably, a microporous filter membrane is fixedly arranged on the filter plate.

Preferably, a rotatable stirrer penetrates through the middle part of the collection tube cover, and a stirring section of the stirrer extends to a position below the liquid level of the cell maintenance liquid.

Preferably, the collection tube cover is further provided with an injection tube in a penetrating manner, one end of the injection tube, which is positioned at the upper side of the collection tube cover, is provided with a section of flexible tube, and a clamp is clamped on the flexible tube.

Preferably, the sample collection assembly comprises a first collection tube, the aerosol concentrator is inserted into the first collection tube and detachably fixed at the upper end of the first collection tube, and the collection tube cover is screwed with the first collection tube; the side wall of the first collecting pipe is connected with a first air exhaust pipe, and the first air exhaust pipe is communicated with the inner cavity of the first collecting pipe and is positioned in the middle of the side edge of the first collecting pipe; and the first air exhaust pipe is externally connected with a second air exhaust pump.

Preferably, the length of the aerosol concentrator is one-half of the length of the first collection tube, the outer diameter of the top end of the aerosol concentrator is less than or equal to the inner diameter of the first collection tube, the top end opening of the aerosol concentrator is provided with an outward bead, the bead is clamped on the edge of the orifice of the first collection tube, and the outer diameter of the bottom end of the aerosol concentrator is less than the outer diameter of the top end of the aerosol concentrator.

Preferably, the sample collection assembly comprises a second collection tube, a second exhaust tube and a sampling tube, the second collection tube is fixedly covered outside the aerosol concentrator, the collection tube cover is screwed on the upper end of the second collection tube, one end of the second exhaust tube, far away from the second collection tube, is fixedly connected with a needle, the outlet end of the sampling tube is sealed with a rubber tube cover, a negative pressure environment is arranged in the sampling tube, and the needle is detachably arranged on the rubber tube cover in a penetrating manner; and a valve is arranged at the middle section of the second exhaust pipe.

Preferably, the aerosol collection assembly is a face mask.

Compared with the prior art, the beneficial effects of the utility model are that: (1) the porous spheroid connected with the air inlet pipe extends into the cell maintenance liquid in the aerosol enricher, and the gas pumped by the first air pump can be fully dispersed in the cell maintenance liquid through the round hole on the porous spheroid, so that the gas and the liquid are fully mixed, and the adsorption and the collection of pathogenic microorganisms are facilitated; (2) part of gas entering the aerosol enricher from the gas inlet pipe emerges from the cell maintenance liquid, the emerging gas is discharged from the gas outlet pipe, and the gas is treated by the filtering component and then is discharged from a pipe orifice at the upper end of the gas outlet pipe, so that secondary pollution to the environment is avoided, and the aerosol in the discharged gas can be effectively filtered; (3) the microporous filter membrane is arranged and used for filtering the cell maintenance liquid which is adsorbed and dissolved with the aerosol from the bottom end of the aerosol concentrator, so that organisms with small particles such as viruses and the like and water enter the sample collection assembly through the microporous filter membrane, and meanwhile, the microporous filter membrane blocks organisms with larger particle sizes such as bacteria and the like from remaining in the aerosol concentrator, so that the separation of the viruses is realized, and the subsequent detection and assay are convenient; (4) the stirrer can further promote the mixing of air and liquid and further prevent the aerosol from being discharged from the air outlet pipe or new aerosol from being generated; (5) the sample collection assembly can sample in two ways, so that the application of the sample collection assembly is more flexible; (6) for the mode of utilizing the second air pump to pump air outwards from the first collecting pipe, negative pressure is formed, so that viruses can easily and directly enter the first collecting pipe through the filter plate to realize liquid separation, and the first sampling pipe can be directly screwed down to sample, so that the sampling dosage can be well controlled; (7) for the negative pressure environment in the sampling tube, the needle head is directly inserted into the sampling tube to suck the cell maintenance liquid (virus solution for short) containing virus inwards, so that the operation is simpler and faster, and the second sampling tube is not required to be disassembled, so that the operation is more convenient; (8) after the virus solution is separated out, injecting cell maintenance liquid into the aerosol concentrator through an injection pipe, wherein the solution in the aerosol concentrator can be used for culturing or detecting large-particle microorganisms such as bacteria and the like; (9) the virus solution separated from the sample sampling assembly can be directly used for virus nucleic acid detection; (10) the gas containing aerosol exhaled by people who speak, cough, sneeze and the like can be directly covered in the face mask by utilizing the face mask, so that the gas containing the aerosol is prevented from diffusing outwards, and the collection of the aerosol gas is facilitated.

Drawings

Fig. 1 is a schematic overall structure diagram of an embodiment of a system for collecting and enriching a microbial aerosol according to the present invention;

fig. 2 is a schematic structural diagram of a porous spheroid of a collection and enrichment system for microbial aerosol according to the present invention;

fig. 3 is a schematic structural diagram of an air outlet pipe of a system for collecting and enriching microbial aerosol according to the present invention;

fig. 4 is a schematic structural diagram of an injection pipe of a collection and enrichment system for microbial aerosol according to the present invention;

fig. 5 is a schematic structural diagram of two embodiments of the collection and enrichment system for microbial aerosol according to the present invention.

Fig. 6 is a schematic structural view of a collecting tube cap of the collecting and enriching system for microbial aerosol according to the present invention.

Fig. 7 is a schematic view of the overall structure of the mask of the collection and enrichment system for microbial aerosol according to the present invention.

Fig. 8 is a side view of the mask of the collection and enrichment system for microbial aerosol of the present invention.

Detailed Description

In order to further understand the objects, structures, features and functions of the present invention, the following embodiments are described in detail.

Referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7 and fig. 8, an embodiment of the present invention provides a system for collecting and enriching a microbial aerosol, which includes an aerosol collecting assembly 6, an aerosol enriching assembly 1 and a sample collecting assembly 2, wherein the aerosol collecting assembly 6 is connected to an input end of the aerosol enriching assembly 1, and the sample collecting assembly 2 is sleeved outside the aerosol enriching assembly 1.

As shown in fig. 1, the aerosol enrichment assembly 1 comprises an aerosol enricher 11 and a collection pipe cover 12, wherein the collection pipe cover 12 is covered on an upper port of the aerosol enricher 11, the aerosol enricher 11 contains a cell maintenance liquid 3, the bottom end of the aerosol enricher 11 is an opening 111, and a filter plate 112 is sealed outside the opening 111; gather and wear to be equipped with into trachea 13 and outlet duct 14 on the tube cap 12, it includes relative first end 131 and second end 132 to go into trachea 13, outlet duct 14 includes relative third end 141 and fourth end 142, first end 131 with third end 141 is located gather the top of tube cap 12, second end 132 with fourth end 142 all is located in aerosol enrichment ware 11, second end 132 fixedly connected with porous spheroid 15, porous spheroid 15 is located in cell maintenance liquid 3, first end 131 is connected with first aspiration pump 4, fourth end 142 is located the liquid level top of cell maintenance liquid 3, detachably cartridge has stopper 143 in the third end 141.

As shown in fig. 1, in the using process of the aerosol enrichment assembly 1 of an embodiment of the present invention, firstly, the aerosol collection assembly 6 is used to collect the microbial aerosol at any time when the aerosol is generated at a position where the aerosol is relatively dense, then the cell maintenance liquid 3 with respect to one half of the volume of the aerosol enrichment assembly is injected into the aerosol enricher 11, the first air pump 4 is turned on, the first air pump 4 has a first air suction pipe 41 and a first exhaust pipe 42 which are opposite, the first exhaust pipe 42 is communicated with the first end 131 of the air inlet pipe 13, the first air pump 4 firstly extracts the gas containing the aerosol to be detected and collected in the aerosol collection assembly 6, the gas containing the aerosol is sucked from the first air suction pipe 41 and discharged from the first exhaust pipe 42, the first exhaust pipe 42 is communicated with the first end 131 of the air inlet pipe 13, so that the gas containing the aerosol directly enters the porous spherical body 15 through the air inlet pipe 13, the gas containing the aerosol is dispersed into the cell maintenance liquid 3 through the porous spherical body 15, so that the microbial aerosol in the gas containing the aerosol is fully contacted with the cell maintenance liquid 3 and is dissolved into the cell maintenance liquid 3, in the process, the first air suction pump 4 continuously pumps the gas containing the aerosol, so that the aerosol is continuously filled into the cell maintenance liquid 3, the concentration of microorganisms in the cell maintenance liquid 3 is favorably improved, the collection of viruses is favorably realized, and a good positive effect is realized on the culture or the detection of the viruses. When the first air pump 4 continuously pumps the gas containing the aerosol into the aerosol enricher 11, the gas containing the aerosol entering the cell maintenance liquid 3 is dispersed by the porous spherical body 15, so that the gas containing the aerosol is fully contacted with the cell maintenance liquid 3, the aerosol is conveniently dissolved in the cell maintenance liquid 3, the gas absorbed by the cell maintenance liquid 3 can bubble out of the cell maintenance liquid 3, the overflowed gas can enter the air outlet pipe 14 through the fourth end 142 of the air outlet pipe 14, and the plug 143 is not plugged into the bottom end at the moment, so that the overflowed gas can smoothly pass through the air outlet pipe 14 and is discharged into the external environment again. Thus, the enrichment work of the aerosol can be completed.

The utility model discloses before carrying out the aerosol enrichment, utilize aerosol collection component 6 to gather through the higher environmental gas of aerosol density, so can be convenient for contain the gas transportation of high density aerosol, the going on of the experiment of being convenient for, for directly installing aerosol collection and enrichment system additional in corresponding the environment, experimental environment is more concentrated, and can effectively practice thrift the inspection equipment cost, can effectively improve aerosol collection and enrichment efficiency simultaneously.

Because the sample collection assembly 2 is sleeved outside the aerosol concentrator 11 and covers the filter plate 112, and meanwhile, the sample collection assembly 2 can create a negative pressure environment relative to the air pressure in the aerosol concentrator 11, after a certain amount of microorganisms capable of meeting detection conditions are enriched in the aerosol concentrator 11, a microorganism solution is sucked from the lower direction under the pushing of the pressure difference between the sample collection assembly 2 and the aerosol concentrator 11, and the solution separation can be realized through the filtering of the filter plate 112. In one embodiment, the filter plate 112 is fixedly mounted with a microporous membrane 113 having a pore size of only 0.2-0.3 μm, or even up to 0.1-0.22. mu.m. Since most of the bacteria have diameters between 0.5 and 5 microns and most of the viruses have diameters less than 0.1 micron, the microporous filter membrane 113 has such a fine pore size that most of the virus particles can only pass through, and meanwhile, most of the bacteria and other large-particle microorganisms are isolated. Therefore, under the action of pressure difference, water and particles smaller than the membrane pores penetrate through the downstream of the filter membrane, the particles larger than the membrane pores are retained on the upper layer of the membrane, after the aerosol is collected, a proper amount of cell maintenance liquid 3 is supplemented into the aerosol concentrator 11 again, the liquid in the aerosol concentrator 11 is taken out for culturing or detecting the particles such as bacteria and the like, and the liquid in the sample collection assembly 2 is used for culturing live viruses or detecting viral nucleic acids.

Before the sample collection assembly 2 extracts the virus solution 5 downwards, a plug 143 needs to be plugged at the third end 141 of the air outlet pipe 14, and the air outlet pipe 14 is sealed, at this time, on one hand, the first air suction pump 4 can be used for continuously pumping the gas containing the aerosol into the aerosol enricher 11, so that the air pressure in the aerosol enricher 11 is increased, and the virus solution 5 is directly pressed into the sample collection assembly 2 through the microporous filter membrane 113; the first air pump 4 can be closed, and only the negative pressure environment made by the sample collection assembly 2 can be used for pumping downwards; or opening the first air pump 4 to continuously pump gold gas into the aerosol enricher 11, and simultaneously matching with the negative pressure environment in the sample collection assembly 2, sucking while pressing, thereby quickly realizing the separation of the virus solution 5.

As shown in fig. 1, a pressure regulator 43 is further disposed at the first discharge pipe 42 of the first suction pump 4 to control the flow rate of the gas containing aerosol charged into the aerosol concentrator 11, so as to ensure that the aerosol in the gas containing aerosol can be sufficiently absorbed, and avoid the situation that the aerosol is discharged without being sufficiently absorbed due to too high inflow flow rate of the gas containing aerosol.

As shown in fig. 2, preferably, a plurality of circular holes 151 are uniformly distributed on the porous spherical body 15, the circular holes 151 penetrate through the porous spherical body 15 and communicate with the air inlet pipe 13, and the circular holes 151 are in contact with the cell maintenance solution 3. Evenly distributed's circular port 151 can effectively increase and the gaseous route that gets into in the cell preservation liquid that contains the aerosol of dispersion, improves the gaseous dispersion effect that gets into the cell preservation liquid that contains the aerosol to effectively increased the area of contact between the gaseous and the cell preservation liquid that contain the aerosol, thereby can improve gas, liquid mixing efficiency.

As shown in fig. 3, preferably, a filter assembly 144 is disposed inside the outlet tube 14, the filter assembly 144 sequentially includes a first filter layer 1441 and a second filter layer 1442 from bottom to top, the first filter layer 1441 is located below the tube cover, and the second filter layer 1442 is located above the sample tube cover. Filter element 144 divide into first filter layer 1441 and second filter layer 1442, when the gas that overflows in the cell preservation liquid gets into outlet duct 14, at first through first filter layer 1441, handle the gas that overflows for the first time, then gaseous rethread second filter layer 1442, further handle the gas that overflows for the second time, the gas after the twice processing of filter element 144 is clean gas and discharges from the mouth of pipe of outlet duct 14 upper end, so can fully filter gas, guarantee gaseous purity. Furthermore, first filter layer 1441 and second filter layer 1442 are both medical filter cotton, so that the medical filter cotton not only has good filtering performance, but also has good air permeability. As shown in fig. 3, the first filter layer 1441 is a double-layer medical filter cotton, the second filter layer 1442 is a single-layer medical filter cotton, the double-layer structure can filter pathogenic microorganisms in the aerosol-containing gas to the maximum extent, and then the aerosol-containing gas is further filtered by the second filter layer 1442 again, so that double filtration and protection are realized.

Preferably, a microporous filter membrane 113 is fixedly mounted on the filter plate 112. The pore diameter of the microporous filter membrane 113 is 0.2-0.3 micron, the microporous filter membrane 113 is usually polyethersulfone (or Cellulose Acetate (CA), Polysulfone (PS), Polyacrylonitrile (PAN), Polyethersulfone (PES)), and can block most of particles such as bacteria through most of virus particles, thereby facilitating analysis of virus micromolecules in the air and reducing influence of large-particle substances such as bacteria

As shown in fig. 1, a rotatable stirrer 16 is preferably inserted into the middle of the collection tube cap 12, and a stirring segment 161 of the stirrer 16 extends below the liquid level of the cell preservation solution 3, so as to further improve the mixing efficiency between the gas containing the aerosol and the cell preservation solution, promote the fusion of the aerosol and the cell preservation solution, and prevent the generation and overflow of new aerosol.

As shown in fig. 1 and 4, preferably, an injection pipe 17 is further inserted into the collection pipe cap 12, a section of flexible pipe 171 is provided at one end of the injection pipe 17 located at the upper side of the collection pipe cap 12, and a clip 172 is clamped on the flexible pipe 171. The injection tube 17 is designed to facilitate injection of the cell maintenance fluid into the aerosol concentrator 11 without removing the collection tube cap 12, which is convenient to operate.

As shown in fig. 1, preferably, the sample collection assembly 2 comprises a primary collection tube 21, the aerosol concentrator 11 is inserted into the primary collection tube 21 and detachably fixed to the upper end of the primary collection tube 21, and the collection tube cap 12 is screwed to the primary collection tube 21; a first air suction pipe 22 is connected to the side wall of the first collection pipe 21, and the first air suction pipe 22 is communicated with the inner cavity of the first collection pipe 21 and is positioned in the middle of the side edge of the first collection pipe 21; the first air suction pipe 22 is externally connected with a second air suction pump 23.

In one embodiment, the sample collection assembly 2 with such a structure is operated by using the second air pump 23 to draw air from the first air suction pipe 22 at the side of the first collection pipe 21, so as to actively form a negative pressure loop inside the first collection pipe 21, such that the water and small particles such as viruses in the aerosol concentrator 11 directly flow into the first collection pipe 21 through the microporous membrane 113 under the action of the pressure difference, thereby completing the separation of live viruses from particles such as bacteria. In this process, it should be noted that, since the first aspirating tube 22 is disposed on the sidewall of the first collecting tube 21, in order to hold enough virus solution 5 in the first collecting tube 21, the first aspirating tube 22 is disposed as close as possible to the upper end of the first collecting tube 21, so that the lower end of the first collecting tube 21 has enough volume to hold the virus solution 5. But at the same time the first suction tube 22 cannot be too far up because of the reduced suction efficiency and the slower speed that would be caused by the obstruction of the aerosol concentrator 11. Therefore, the preferred scheme is to arrange the first air suction pipe 22 below the aerosol concentrator 11, which not only ensures the air suction efficiency, but also enables the first collection pipe 21 to have a larger capacity for containing the sample solution, and meanwhile, can prevent the solution from overflowing into the second air suction pump 23 from the interface between the first air suction pipe 22 and the first collection pipe 21, thereby ensuring the normal operation of the device.

As shown in fig. 1, preferably the aerosol concentrator 11 has a length of one half of the length of the first collection tube 21, the aerosol concentrator 11 has a top end with an outer diameter smaller than or equal to the inner diameter of the first collection tube 21, the top end opening of the aerosol concentrator 11 is provided with an outward bead, the bead is snapped onto the orifice edge of the first collection tube 21, and the outer diameter of the bottom end of the aerosol concentrator 11 is smaller than the outer diameter of the top end of the aerosol concentrator 11. With the structure, on one hand, the aerosol concentrator 11 can be conveniently inserted into the first collecting pipe 21, and on the other hand, the situation that the aerosol concentrator 11 cannot be taken out easily when sliding into the first collecting pipe 21 can be avoided. Since the length of aerosol concentrator 11 is only one-half of that of first collection tube 21, and the volume of cell maintenance liquid 3 in aerosol concentrator 11 is one-half of that of aerosol concentrator 11, and first suction tube 22 is located in the middle of the side wall of first collection tube 21, even if the solution in aerosol concentrator 11 is completely sucked out, the sucked virus solution 5 is not hidden from the mouth of first suction tube 22, and the equipment can be protected from damage.

As shown in fig. 5, preferably, the sample collection assembly 2 includes a second collection tube 24, a second suction tube 25 and a sampling tube 26, the second collection tube 24 is fixedly covered outside the aerosol concentrator 11, the collection tube cap 12 is screwed on the upper end of the second collection tube 24, one end of the second suction tube 25 far from the second collection tube 24 is fixedly connected with a needle 27, the outlet end of the sampling tube 26 is sealed with a rubber tube cap 261, a negative pressure environment is provided in the sampling tube 26, and the needle 27 is detachably inserted on the rubber tube cap 261. Furthermore, a valve 28 is disposed in the middle of the second suction pipe 25.

In the second embodiment, when the needle 27 is inserted into the sampling tube 26 and the second collection tube 24 is communicated with the sampling tube 26, due to the negative pressure environment inside the sampling tube 26, the negative pressure environment is also formed inside the second collection tube 24, and the pressure is relatively small compared with the air pressure inside the aerosol concentrator 11, so that the second collection tube 24 can downwardly attract the virus solution 5 to flow into the second collection tube 24 under the action of the difference between the upper pressure and the lower pressure, and the second suction tube 25 is located at the bottom end of the second collection tube 24, so that the virus solution 5 flows into the sampling tube 26 along the second suction tube 25 when flowing to the bottom of the second collection tube 24, and thus the collection of the virus solution 5 sample can be completed. The valve 28 is arranged to lock the second suction tube 25 when the sample is not required to be collected, so as to seal the second collection tube 24 and prevent the virus solution 5 from flowing out. When sampling is required, the needle 27 is inserted into the rubber tube cover of the sampling tube 26, and the valve 28 is opened, so that the virus solution 5 can be automatically sucked. Of course, the operation of plugging the plug 143 and continuously pumping the aerosol-containing gas into the aerosol concentrator 11 by the first air pump 4 may also be performed, so that the pressure difference can be increased, the virus solution 5 can be rapidly separated, and rapid sampling can be realized. Need not open second collection pipe 24 this moment and can take out virus solution 5, convenient and fast, and need not worry about the anti-condition of overflowing of virus solution 5, the structure is simpler simultaneously, saves equipment cost, and sampling pipe 26 connects and can directly transport testing platform after getting virus solution 5, cultivates and detects convenient and fast more. It should be noted that, due to the good fluidity of the rubber, when the needle 27 is pulled out, the hole of the rubber tube cover 261 pierced by the needle 27 will be automatically pressed and closed by the autonomous flow of the rubber, so that the sampling tube 26 returns to the sealed environment.

Meanwhile, as a large number of samples are needed in the experiment or detection process, in the second embodiment, the sample collection assembly 2 is matched with the aerosol enrichment assembly 1, continuous sampling can be carried out under the condition that the cover of the sampling tube 26 is not disassembled, and the device is more convenient and efficient. The specific operation method comprises the following steps: opening the plug 143 to enable the gas outlet pipe 14 to degas smoothly, then directly and continuously injecting the cell maintenance liquid 3 into the aerosol enricher 11 through the injection pipe 17, wherein the virus content in the cell maintenance liquid 3 is low, then opening the first air pump 4 to continuously pump the gas containing the aerosol into the aerosol enricher 11, wherein the gas containing the aerosol is dispersed in the cell maintenance liquid 3 through the porous spheroid 15 and is dissolved in water when fully contacting with the cell maintenance liquid 3, and simultaneously the stirrer 16 rotates to stir the downward stirring maintenance liquid in the whole aerosol enricher 11, so that the cell maintenance liquid 3 and the gas containing the aerosol are further fully contacted and mixed, the water dissolution rate of aerosol microorganisms is improved, the gas containing the aerosol is prevented from directly overflowing the cell maintenance liquid 3 or generating new aerosol to be discharged out of the aerosol enricher 11, then opening the valve 28, the needle 27 is inserted into the sampling tube 26, and the vacuum environment of the sampling tube 26 is used to directly perform suction, and the virus solution 5 flows through the microporous membrane 113 under the vacuum environment, enters the second collection tube 24 and further flows into the sampling tube 26. At this moment, the sampling tube 26 continuously pumps, the injection tube 17 continuously injects, and the porous spheroid 15 is ensured to be always positioned below the liquid level of the cell maintenance liquid 3, so that the gas containing the aerosol can be always in contact with the cell maintenance liquid 3, meanwhile, the gas containing the aerosol is continuously pumped into the first air pump 4, then the stirrer 16 continuously stirs, at this moment, after the sampling tube 26 pumps out the specified amount of virus solution 5, the sampling tube 26 can be pulled out, and then a new empty sampling tube 26 is connected to continue to extract the virus solution 5, so that continuous sampling is realized. And stopping sampling until sampling is finished or the microfiltration membrane 113 loses filtering capacity, closing the valve 28, the first air pump 4 and the stirrer 16, continuing to inject the cell maintenance liquid 3 into the aerosol enricher 11 through the injection pipe 17 to provide living environment for the microorganisms such as bacteria, stopping injecting when the cell maintenance liquid 3 is injected to about one half of the volume of the aerosol enricher 11, clamping the clamp 172, and taking out the solution in the aerosol enricher 11 to culture and detect the microorganisms such as bacteria and the like with large particles.

As shown in fig. 6, it is noted that in both embodiments, collection tube cap 12 has the same structure, and has an inner layer 121 and an outer layer 122, where inner layer 121 is a rubber sealing plug for sealing the opening of aerosol concentrator 11 to ensure a sealed environment inside the aerosol concentrator, and outer layer 122 is screwed onto the outside of the opening of first collection tube 21 or second collection tube 24 to ensure the sealing of the opening of the collection tubes. Meanwhile, the air inlet pipe 13, the air outlet pipe 14, the injection pipe 17 and the stirrer 16 are all arranged on the inner layer, namely the sealing rubber plug in a penetrating way, so that the air inlet pipe, the air outlet pipe 14, the injection pipe 17 and the stirrer 16 are all positioned in the aerosol concentrator 11.

In addition, the first air pump 4 and the second air pump 23 can be of heavier sizes and have various sizes, and only the input and output pipelines of the first air pump and the second air pump need to be correspondingly and hermetically connected with the corresponding pipelines in the system.

As shown in fig. 7 and 8, preferably, the aerosol collection assembly 6 is a face mask. The mask comprises a first mask body 61, a second mask body 62 and a connecting structure 63, wherein the first mask body 61 comprises a side opening structure 611, an air inlet 612, a fixing strip 613 and a rib 614, the side opening structure 611 is located on the left side of the first mask body 61, the air inlet 612 is located in the middle position of the first mask body 61, the fixing strip 613 is fixed on two side edges of the side opening structure 61, the rib 614 is fixed on the side edge and the bottom edge of the first mask body 61 and surrounds to form a U-shaped structure, and the first mask body 61 is a quarter cylinder.

The second cover 62 comprises a bottom opening 621, an air outlet 622 and a delivery pipe 623, the bottom opening 621 is located at the lower side of the second cover 62, the air outlet 622 is located at the upper left of the second cover 62, the air outlet 622 is connected with the delivery pipe 623, and the second cover 62 is a hollow hemisphere; the second cover 62 is transversely placed above the first cover 61, the first cover 61 is vertically arranged below the second cover 62, and the first cover 61 and the second cover 62 are communicated with each other; one end of the connecting structure 63 is connected to the top end of the first cover 61, and the other end is connected to the bottom edge of the second cover 62; the side opening structure 611 and the bottom opening 621 are perpendicular to each other; when the mask is used, a person to be collected holds the side opening structure 611 of the first mask body 61 with one hand and holds the bottom opening 621 of the second mask body 62 with the other hand, and the mask is put on the head, when the size of the opening formed by the side opening structure 611 and the bottom opening 621 is not suitable for the size of the head of the person to be collected, the engaging structure 63 can be stretched, and because the engaging structure 63 has elasticity, the size of the opening can be adjusted to suit the size of the head of different persons to be collected, and then the positions of the edge blocking strip 614 and the face contour can be adjusted, the edge blocking strip 614 has elasticity, is in accordance with the human engineering, can perfectly fit with the face contour, and can avoid the aerosol generated by talking spray, sneeze and cough from escaping from the side opening structure 611, and meanwhile, the elastic arrangement of the edge blocking strip 614 can also increase the comfort level of the person to be collected to wear the mask, avoiding the face of the collecting personnel from being crushed; then, the fixing bands 613 on the two sides of the side opening structure 611 are wound to the position of the back of the head for knotting and fastening, so that the shaking of the collecting mask caused by the rotation of the head of a human body is avoided, and the fixing performance is enhanced; collected personnel inhale fresh gas through the air inlet 6121 on the air inlet 612 to prevent oxygen deficiency, gas generated by talking spray, sneeze, cough and the like can pass through the air outlet 622 on the upper left of the second cover body 62 and then is discharged through the delivery pipe 623, and the other end of the delivery pipe 623 is connected with the first air pump 4 to pump out the gas and enter the aerosol enrichment assembly 1 to perform the enrichment work of aerosol.

Referring to fig. 8, fig. 8 is a side view of the mask for collecting pathogenic microorganism samples of the present invention. Preferably, the engaging structure 63 is a V-shaped structure, one side of the engaging structure is connected to the first cover 61, and the other side of the engaging structure is connected to the second cover 62, and the engaging structure 63 has elasticity, so that the size of the opening positions of the first cover 61 and the second cover 62 can be adjusted, and the engaging structure is suitable for people with different head sizes to wear. And the linking structure 63 is an elastic structure, can be automatically adapted to the size of the head of the acquisition personnel, and is more convenient to adjust.

Furthermore, an elastic sealing strip 64 is arranged at a gap at the joint of the upper edge of the first cover body 61 and the lower edge of the second cover body 62, the length of the elastic sealing strip 64 is equal to the arc length of the upper edge of the first cover body 61, and two sides of the elastic sealing strip 64 are respectively sealed and adhered to the first cover body 61 and the second cover body 62, so that the gap at the joint of the first cover body 61 and the second cover body 62 is sealed, aerosol is prevented from overflowing outwards from the joint when the aerosol is collected, the elastic sealing strip 64 can be directly extruded and deformed when the first cover body 61 is upwards opened, and the whole structure cannot be influenced.

First cover body 61 bottom is convex saddle 615 of downward concave for catch up gather personnel's chin, and the collocation seals when strake 614, so when first cover body 61 outwards breaks the angle between adjustment and the second cover body 62 off with the fingers and thumb, saddle 615 homoenergetic catches up gather personnel's side face and chin from gather personnel's chin bottom, avoids gathering the gaseous majority of personnel's exhalation and spills over.

Preferably, the shape of the air inlet 612 is triangular, the air inlet 612 is provided with a plurality of air inlet holes 6121, the air inlet holes 6121 are of a circular structure, and the air inlet holes 6121 can enable a wearer to inhale fresh air, so that oxygen deficiency is prevented, and secondary injury is avoided. The air inlet 612 is located in the middle of the first mask body 61 and is located near the nose of the collecting person when the collecting person wears the mask, so that the collecting person can breathe conveniently.

Preferably, the edge blocking strips 614 are of a continuous S-shaped structure, the material of the edge blocking strips 614 is an elastic rubber strip or a sponge strip, the comfort level of the acquisition mask worn by the acquisition personnel can be increased by the elastic rubber strip or the sponge strip, and the tightness between the acquisition mask and the surface contact can also be increased.

The collection of aerosol is carried out through the face guard, can directly collect the pathogenic microorganism of aerosol that the droplet of sick personnel when sneezing, cough or speaking produced and collect for aerosol collection efficiency is higher, is more convenient for the enrichment of aerosol to gather.

From top to bottom, the utility model discloses a collection and enrichment system of microorganism aerosol: (1) the porous spheroid connected with the air inlet pipe extends into the cell maintenance liquid in the aerosol enricher, and the gas pumped by the first air pump can be fully dispersed in the cell maintenance liquid through the round hole on the porous spheroid, so that the gas and the liquid are fully mixed, and the adsorption and the collection of pathogenic microorganisms are facilitated; (2) part of gas entering the aerosol enricher from the gas inlet pipe emerges from the cell maintenance liquid, the emerging gas is discharged from the gas outlet pipe, and the gas is treated by the filtering component and then is discharged from a pipe orifice at the upper end of the gas outlet pipe, so that secondary pollution to the environment is avoided, and the aerosol in the discharged gas can be effectively filtered; (3) the microporous filter membrane is arranged and used for filtering the cell maintenance liquid which is adsorbed and dissolved with the aerosol from the bottom end of the aerosol concentrator, so that organisms with small particles such as viruses and the like and water enter the sample collection assembly through the microporous filter membrane, and meanwhile, the microporous filter membrane blocks organisms with larger particle sizes such as bacteria and the like from remaining in the aerosol concentrator, so that the separation of the viruses is realized, and the subsequent detection and assay are convenient; (4) the stirrer can further promote the mixing of air and liquid and further prevent the aerosol from being discharged from the air outlet pipe or new aerosol from being generated; (5) the sample collection assembly can sample in two ways, so that the application of the sample collection assembly is more flexible; (6) for the mode of utilizing the second air pump to pump air outwards from the first collecting pipe, negative pressure is formed, so that viruses can easily and directly enter the first collecting pipe through the filter plate to realize liquid separation, and the first sampling pipe can be directly screwed down to sample, so that the sampling dosage can be well controlled; (7) the virus solution can be absorbed inwards by directly inserting the needle into the sampling tube under the negative pressure environment in the sampling tube, so that the sampling tube is simpler and quicker, and the second sampling tube is not required to be disassembled, so that the operation is more convenient; (8) after the virus solution is separated out, injecting cell maintenance liquid into the aerosol concentrator through an injection pipe, wherein the solution in the aerosol concentrator can be used for culturing or detecting large-particle microorganisms such as bacteria and the like; (9) the virus solution separated from the sample sampling assembly can be directly used for virus nucleic acid detection, so that the detection result is more accurate; (10) utilize the face guard can directly cover the gas of people's exhalation in the face guard, avoid the gas that contains the aerosol to spread outward, the gaseous collection of the aerosol of being more convenient for.

The present invention has been described in relation to the above embodiments, which are only examples for implementing the present invention. It should be noted that the disclosed embodiments do not limit the scope of the invention. On the contrary, all changes and modifications which do not depart from the spirit and scope of the present invention are deemed to fall within the scope of the present invention.

Claims (10)

1. A collection and enrichment system of microbial aerosol is characterized in that: the device comprises an aerosol collecting component, an aerosol enriching component and a sample collecting component, wherein the aerosol collecting component is connected to the input end of the aerosol enriching component, and the sample collecting component is sleeved on the outer side of the aerosol enriching component;

the aerosol enrichment assembly comprises an aerosol enrichment device and a collection pipe cover, the collection pipe cover is mounted on the upper port of the aerosol enrichment device, cell maintenance liquid is contained in the aerosol enrichment device, the bottom end of the aerosol enrichment device is open, and a filter plate is packaged outside the open end; gather and wear to be equipped with into trachea and outlet duct on the pipe cover, it includes relative first end and second end to go into the trachea, the outlet duct includes relative third end and fourth end, first end with the third end is located gather the top of pipe cover, the second end with the fourth end all is located in the aerosol enrichment ware, the porous spheroid of second end fixedly connected with, the porous spheroid is located in the cell maintenance liquid, first end is connected with first aspiration pump, the fourth end is located the liquid level top of cell maintenance liquid, detachably cartridge has the stopper in the third end.

2. The microbial aerosol collection and enrichment system of claim 1, wherein: a plurality of circular holes are uniformly distributed on the porous spheroid, the circular holes penetrate through the porous spheroid and are communicated with the air inlet pipe, and the circular holes are in contact with the cell maintenance liquid.

3. The microbial aerosol collection and enrichment system of claim 1, wherein: the inside filtering component that is provided with of outlet duct, filtering component includes first filter layer and second filter layer by supreme down in proper order, first filter layer is located the below of tube cap, the second filter layer is located the top of appearance tube cap.

4. The microbial aerosol collection and enrichment system of claim 1, wherein: and a microporous filter membrane is fixedly arranged on the filter plate.

5. The microbial aerosol collection and enrichment system of claim 1, wherein: rotatable stirring pieces penetrate through the middle of the collecting pipe cover, and stirring sections of the stirring pieces extend to the position below the liquid level of the cell maintenance liquid.

6. The microbial aerosol collection and enrichment system of claim 1, wherein: the collecting pipe cover is also provided with an injection pipe in a penetrating way, one end of the injection pipe, which is positioned at the upper side of the collecting pipe cover, is provided with a section of hose, and a clamp is clamped on the hose.

7. A microbial aerosol collection and enrichment system according to any of claims 1 to 6, wherein: the sample collection assembly comprises a first collection pipe, the aerosol concentrator is inserted into the first collection pipe and detachably fixed at the upper end of the first collection pipe, and the collection pipe cover is screwed with the first collection pipe; the side wall of the first collecting pipe is connected with a first air exhaust pipe, and the first air exhaust pipe is communicated with the inner cavity of the first collecting pipe and is positioned in the middle of the side edge of the first collecting pipe; and the first air exhaust pipe is externally connected with a second air exhaust pump.

8. The microbial aerosol collection and enrichment system of claim 7, wherein: the length of the aerosol concentrator is one-half of the length of the first collection tube, the outer diameter of the top end of the aerosol concentrator is less than or equal to the inner diameter of the first collection tube, an outward curled edge is arranged at the top end opening of the aerosol concentrator, the curled edge is clamped on the edge of the tube opening of the first collection tube, and the outer diameter of the bottom end of the aerosol concentrator is less than the outer diameter of the top end of the aerosol concentrator.

9. A microbial aerosol collection and enrichment system according to any of claims 1 to 6, wherein: the sample collection assembly comprises a second collection pipe, a second exhaust pipe and a sampling pipe, the second collection pipe is fixedly covered on the outer side of the aerosol concentrator, the collection pipe cover is screwed on the upper end of the second collection pipe, one end, far away from the second collection pipe, of the second exhaust pipe is fixedly connected with a needle head, the outlet end of the sampling pipe is sealed with a rubber pipe cover, a negative pressure environment is arranged in the sampling pipe, and the needle head is detachably arranged on the rubber pipe cover in a penetrating mode; and a valve is arranged at the middle section of the second exhaust pipe.

10. The microbial aerosol collection and enrichment system of claim 1, wherein: the aerosol collection assembly is a face mask.

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