CN111633018A - Heavy metal contaminated soil remediation device and bioremediation method - Google Patents
Heavy metal contaminated soil remediation device and bioremediation method Download PDFInfo
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- CN111633018A CN111633018A CN202010518070.1A CN202010518070A CN111633018A CN 111633018 A CN111633018 A CN 111633018A CN 202010518070 A CN202010518070 A CN 202010518070A CN 111633018 A CN111633018 A CN 111633018A
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Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a heavy metal contaminated soil remediation device and a bioremediation method, and the heavy metal contaminated soil remediation device comprises a horizontally arranged mounting seat, wherein two symmetrically arranged moving mechanisms are arranged on the side wall of the lower side of the mounting seat, a mounting box is arranged on the side wall of the upper side of the mounting seat, a first communication hole communicated with the outside is formed in the upper end of the mounting box, a box cover matched with the first communication hole is arranged at the upper end of the mounting box, a mixing mechanism is arranged in the mounting box, limiting plates matched with the mixing mechanism are arranged on the inner walls of two sides of the mounting box, a first conveying mechanism and a second conveying mechanism are respectively arranged on two sides of the mounting box, and the first conveying mechanism and the second conveying mechanism are matched with the mixing mechanism. The invention improves the soil remediation effect by matching two different soil remediation means.
Description
Technical Field
The invention relates to the technical field of soil remediation, in particular to a heavy metal contaminated soil remediation device and a bioremediation method.
Background
The soil inorganic pollutants are more prominent in heavy metals, and are easy to accumulate because the heavy metals cannot be decomposed by soil microorganisms, and are converted into methyl compounds with higher toxicity, even some methyl compounds are accumulated in a human body through a food chain at harmful concentration and seriously harm the health of the human body, the soil heavy metal pollutants mainly comprise mercury, cadmium, lead, copper, chromium, arsenic, nickel, iron, manganese, zinc and the like, although the arsenic does not belong to the heavy metals, the behavior, the source and the harm of the arsenic are similar to those of the heavy metals, and the heavy metals are generally listed for discussion. As far as the needs for plants are concerned, the metallic elements can be classified into 2 types: the method comprises the following steps of firstly, elements which are not needed for the growth and development of plants and have obvious harm to human health, such as cadmium, mercury, lead and the like, secondly, elements which are needed for the normal growth and development of plants and have certain physiological functions to human bodies, such as copper, zinc and the like, but the elements can cause pollution too much to hinder the growth and development of plants, and the same metals are different in migration and transformation characteristics and pollution properties because of different forms in soil, so when the harm of heavy metals in soil is researched, not only the total content of the heavy metals is paid attention to, but also the content of various forms must be paid attention to, and a soil restoration device is needed to be used for soil restoration;
the existing soil remediation device has a single soil remediation means and a poor soil remediation effect, so that a heavy metal contaminated soil remediation device and a bioremediation method are provided to solve the problems.
Disclosure of Invention
The invention aims to solve the problems of single soil remediation means and poor soil remediation effect of a soil remediation device in the prior art, and provides a heavy metal contaminated soil remediation device and a bioremediation method.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a heavy metal contaminated soil prosthetic devices, includes the mount pad that the level set up, the downside lateral wall of mount pad is equipped with the moving mechanism that two symmetries set up, the upside lateral wall of mount pad is equipped with the install bin, the upper end of install bin is equipped with the first communicating hole with outside intercommunication, and the upper end of install bin is equipped with the case lid that matches the setting with first communicating hole, be equipped with the hybrid mechanism in the install bin, and the both sides inner wall of install bin all is equipped with the limiting plate that matches the setting with the hybrid mechanism, the both sides of install bin are equipped with first conveying mechanism and second conveying mechanism respectively, and first conveying mechanism and second conveying mechanism all match the setting with the hybrid mechanism.
Preferably, the equal fixedly connected with of both sides lateral wall of case lid first inserted bar of vertical setting, and the both sides lateral wall of install bin all is equipped with the slot that matches the setting with first inserted bar.
Preferably, mixing mechanism includes the mixing box, the upper end of mixing box is equipped with the second intercommunicating pore with outside intercommunication, and the upper end of mixing box is equipped with the closing cap that sets up with the second intercommunicating pore matching, be equipped with driving motor on the case lid, and driving motor's output runs through the lateral wall and the activity joint of case lid and closing cap in proper order has the pivot, the both sides of pivot all are equipped with a plurality of flabellums.
Preferably, the moving mechanism comprises two symmetrically arranged pulleys, and the side wall of one side of each of the two pulleys, which is opposite to the other side, is provided with a telescopic supporting seat which is matched with the pulleys.
Preferably, first conveying mechanism includes first delivery board, first delivery board is hollow structure, and the upper end of first delivery board is equipped with first delivery pump, the input of first delivery pump runs through the lateral wall setting of install bin and mixing box in proper order, and the output of first delivery pump and the inside intercommunication of first delivery board, the downside lateral wall of first delivery board is equipped with the third intercommunicating pore with outside intercommunication, be equipped with the dispersion net in the third intercommunicating pore, the both sides lateral wall of first delivery board all inserts and is equipped with the second inserted bar that matches the setting with the dispersion net, and the both sides lateral wall of first delivery board all is equipped with the buckle that matches the setting with the second inserted bar.
Preferably, second conveying mechanism includes the second delivery board, and the second delivery board is hollow structure, the upper end of second delivery board is equipped with the second delivery pump, the input of second delivery pump runs through the lateral wall setting of install bin and mixing box in proper order, and the output and the inside intercommunication of second delivery board of second delivery pump, insert on the second delivery board and be equipped with a plurality of flexible conveyer pipes, and a plurality of flexible conveyer pipes all communicate with the second delivery board is inside.
A bioremediation method of heavy metal contaminated soil comprises the following steps:
fungus repair:
1) arbuscular mycorrhizal fungi.
2) A fungus of the genus trichoderma.
3) A yeast.
And (4) repairing functional bacteria.
The invention has the following beneficial effects: the plurality of pulleys are matched to facilitate the movement of the repairing device, the plurality of telescopic supporting seats are matched to facilitate the fixed placement of the repairing device, one or more of arbuscular mycorrhizal fungi, trichoderma fungi, saccharomycetes and functional bacteria and a culture medium are added into a mixing box together, a driving motor is started to drive a rotating shaft and fan blades to rotate, so that one or more of arbuscular mycorrhizal fungi, trichoderma fungi, saccharomycetes and functional bacteria and the culture medium are fully cultured after being mixed, a first conveying pump can convey a liquid repairing agent into a first conveying plate, the liquid repairing agent is sprayed on the polluted soil after being dispersed through a dispersion net to repair the soil, a second conveying pump can convey a solid repairing agent into a second conveying plate, the solid repairing agent is conveyed into the polluted soil through a telescopic conveying pipe to repair the polluted soil, and two different soil repairing means are matched, improving the soil remediation effect.
Drawings
FIG. 1 is a schematic structural diagram of a heavy metal contaminated soil remediation device provided by the invention;
FIG. 2 is a schematic side view of the heavy metal contaminated soil remediation device according to the present invention;
FIG. 3 is a schematic view of the structure at A in FIG. 1;
FIG. 4 is a schematic view of the structure at B in FIG. 2;
fig. 5 is a schematic structural diagram at C in fig. 1.
In the figure: the device comprises a mounting base 1, a mounting box 2, a box cover 3, a limiting plate 4, a first inserted link 5, a mixing box 6, a sealing cover 7, a driving motor 8, a rotating shaft 9, fan blades 10, pulleys 11, a telescopic supporting seat 12, a first conveying plate 13, a first conveying pump 14, a dispersing net 15, a second inserted link 16, a buckle 17, a second conveying plate 18, a second conveying pump 19 and a telescopic conveying pipe 20.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-3, a heavy metal contaminated soil remediation device comprises a horizontally arranged mounting base 1, wherein two symmetrically arranged moving mechanisms are arranged on the lower side wall of the mounting base 1, a mounting box 2 is arranged on the upper side wall of the mounting base 1, a first communication hole communicated with the outside is arranged at the upper end of the mounting box 2, a box cover 3 matched with the first communication hole is arranged at the upper end of the mounting box 2, a mixing mechanism is arranged in the mounting box 2, a limiting plate 4 matched with the mixing mechanism is arranged on the inner wall of each of two sides of the mounting box 2, a first conveying mechanism and a second conveying mechanism are respectively arranged on each of two sides of the mounting box 2, the first conveying mechanism and the second conveying mechanism are matched with the mixing mechanism, a first inserted rod 5 vertically arranged is fixedly connected to each of two side walls of the box cover 3, and slots matched with the first inserted rod 5 are arranged on each of two side walls of the mounting box 2, the mixing mechanism comprises a mixing box 6, a second communication hole communicated with the outside is arranged at the upper end of the mixing box 6, a sealing cover 7 matched with the second communication hole is arranged at the upper end of the mixing box 6, a driving motor 8 is arranged on the box cover 3, the output end of the driving motor 8 sequentially penetrates through the side walls of the box cover 3 and the sealing cover 7 and is movably clamped with a rotating shaft 9, a plurality of fan blades 10 are arranged on two sides of the rotating shaft 9, the moving mechanism comprises two symmetrically arranged pulleys 11, a telescopic supporting seat 12 matched with the pulleys 11 is arranged on the side wall on the back of the two pulleys 11, the first conveying mechanism comprises a first conveying plate 13, the first conveying plate 13 is of a hollow structure, a first conveying pump 14 is arranged at the upper end of the first conveying plate 13, the input end of the first conveying pump 14 sequentially penetrates through the side walls of the mounting box 2 and the mixing box 6, and the output end of the first conveying pump 14 is communicated with the inside of, the side wall of the lower side of the first conveying plate 13 is provided with a third communicating hole communicated with the outside, a dispersion net 15 is arranged in the third communicating hole, the side walls of the two sides of the first conveying plate 13 are respectively inserted with a second inserting rod 16 matched with the dispersion net 15, the side walls of the two sides of the first conveying plate 13 are respectively provided with a buckle 17 matched with the second inserting rod 16, the second conveying mechanism comprises a second conveying plate 18, the second conveying plate 18 is of a hollow structure, the upper end of the second conveying plate 18 is provided with a second conveying pump 19, the input end of the second conveying pump 19 sequentially penetrates through the side walls of the mounting box 2 and the mixing box 6, the output end of the second conveying pump 19 is communicated with the inside of the second conveying plate 18, the second conveying plate 18 is inserted with a plurality of telescopic conveying pipes 20, the telescopic conveying pipes 20 are respectively communicated with the inside of the second conveying plate 18, the plurality of pulleys 11 are matched to facilitate the movement of the repairing device, the plurality of telescopic supporting seats 12 are matched to facilitate the fixed placement of the repairing device, one or more of arbuscular mycorrhizal fungi, trichoderma fungi, saccharomycetes and functional bacteria and a culture medium are added into the mixing box 6, the driving motor 8 is started to drive the rotating shaft 9 and the fan blades 10 to rotate, so that one or more of arbuscular mycorrhizal fungi, trichoderma fungi, saccharomycetes and functional bacteria and the culture medium are fully cultured after being mixed, the first conveying pump 14 can convey the liquid repairing agent into the first conveying plate 13, the liquid repairing agent is sprayed on the polluted soil after being dispersed through the dispersing net 15 to repair the soil, the second conveying pump 19 can convey the solid repairing agent into the second conveying plate 18, the solid repairing agent is conveyed into the polluted soil through the telescopic conveying pipe 20 to repair the polluted soil, and two different soil repairing means are matched, the soil remediation effect is improved;
a bioremediation method of heavy metal contaminated soil comprises the following steps:
fungus repair:
1) arbuscular mycorrhizal fungi: arbuscular mycorrhizal fungi are a kind of beneficial microorganism widely distributed in soil ecosystem, can form symbiont with more than 90% of terrestrial higher plants, under the condition of heavy metal pollution, AM fungi can help host plants to reduce the absorption of heavy metals to avoid their toxic action, or enhance the tolerance of host plants to heavy metal stress in soil, ryegrass has very strong adsorption capacity to heavy metal Cd, it has been proved by research that AM fungi can increase the overground part, underground part and total biomass of ryegrass, thus the accumulation amount H of Cd, Cd in ryegrass is significantly increased, study of Cozzolino and the like shows that the quality of lettuce in potting test can be effectively enhanced by adopting the mode of combining humus and inoculated AM fungi, and the toxic action of mercury to the lettuce is reduced, study of Jiang and the like conducted by branched fungi Glomussversaford (GV) and Rhi. The results show that: GV and Ri can obviously increase the biomass of the overground part and root system of all the honeysuckle subjected to cadmium treatment, GV treatment can obviously reduce the cadmium content of plant buds and roots, Ri treatment can reduce the cadmium content of the plant buds but increase the cadmium content of the roots, meanwhile, compared with the treatment without GV and Ri inoculation, the contents of Catalase (CAT), ascorbic acid oxidase (APX), Glutathione Reductase (GR), ascorbic acid (ASA) and Phytochelatin (PC) in the honeysuckle are obviously increased, but the content of Malondialdehyde (MDA) and Glutathione (GSH) is reduced, the symbiotic system of honeysuckle with GV and Ri reduces the cadmium content of the upper part of the ground to relieve the cadmium toxicity, and improves the absorption of phosphorus, and the research of Mishra and the like shows that the AM fungus has good effect on the remediation of iron-contaminated soil of the plants for the remediation of the imperial cereals and the sorghum, under potting conditions, the AM fungus is able to produce siderophores to promote Fe uptake by plants.
2) Trichoderma fungi: trichoderma fungi are various in variety, are widely distributed in soil, plant rhizosphere, sapropel or other matrixes, are easy to screen and separate, have strong environmental adaptability, and have various functions related to copper pollution remediation, such as promotion of plant growth, copper resistance, enhancement of plant copper stress resistance, copper accumulation property, enhancement of soil copper effectiveness and the like of copper resistance of Trichoderma reesei FS10-C, Trichoderma harzianum has the maximum degradation rate of cd & lt 58 & gt after condition optimization at the pH value of 4 and the temperature of 28 ℃, heavy metal-resistant rape endophytic fungi Fusarium sp.CBRF14, Penicillium sp.CBRF65 and Awaaisp.CBSF68, have the maximum enrichment capacity of cd between 20.5 and 53.4121g/g, the maximum enrichment capacity of Pb between 188.9 and 356.6mg/g, have the maximum enrichment capacity of zn between 28.4 and 292.8mS/g, have the best adsorption effect of Rhizopus oryzae spores on Fe & lt, and are prepared into a composite SO material by combining the two kinds of CumS and Fe & lt 292.8, the color becomes lighter, which indicates that the composite ions have adsorption effect on cu. The results of comparison of 3 strains of fungi, namely Aspergillus onii (Aspergillus flavus), Trichoderma harzianum (Trichoderma harzianum) and Gliocladium roseum (Clinostachysea) on the biological enrichment capacity of Ag show that Trichoderma harzianum is the strain with the strongest tolerance and enrichment capacity on Ag, and can be enriched by 153ms/L under the condition that the Ag concentration is 330 mg/L. The Trichoderma harzianum strain screened and separated from the barren soil highly polluted by Cu near the abandoned Fe-Cu sulfur ore by the Zotti et al shows stronger enrichment capacity to Cu, and is 19628 mg/kg.
3) Yeast: saccharomyces cerevisiae is taken as a control strain, and P.kudriavzevii A16 has obvious Cd, Cu and Zn resistance and accumulation characteristics, the removal rate of Cd with the concentration lower than 0.04mmol/L and zn with the concentration of 0.1mmol/L exceeds 95 percent, can obviously improve the cadmium resistance of the yeast strain under high salt or low pH value, but has reduced resistance to copper, the zinc resistance has no obvious change, and the cadmium resistance mechanism of yeast strains shows that the expression of antioxidant enzyme genes such as SODC, SODM, PRXI, PRX2D, CATA and the like under the cadmium stress of P.kudriavzevii A16 can be obviously improved under the high salt or low pH value, thereby reducing the ROS content in yeast cells, the cell death rate, the lipid peroxidation level in the cells, the protein oxidation damage level and the like, and Kluyveromyces kluyveromyces marxianus and Rhodotorula mucilaginosa have good tolerance and adsorptivity to cadmium, when the concentration of Cd is 50ITIg/L, the growth OD flails are all larger than 5.0, and the adsorption rate of the grown OD flails to cadmium in the solution after 24 hours is more than 70%. Anh, etc., and the growth of the wild type mutant Saccharomyces cerevisiae strain IM3 in YPD fermentation liquid containing 15mmoWLMn is not inhibited, and the accumulation amount of Mn is 4 times of that of the wild type strain BY 4741.
Functional bacteria repair: the research on the effect of the mixed microbial preparation of bacillus megaterium and bacillus mucilaginosus on the adsorption of heavy metals of ultra-enriched plant Indian mustard is researched through a pot experiment, and the result shows that: the mixed microbial preparation not only promotes the growth of the Indian mustard, but also enhances the absorption of heavy metals Cd, Pb and Zn by hyper-enriched plants, greatly improves the phytoremediation efficiency, and finds that the adsorption performance of a strain E (Citrobacter) is optimal through adsorption performance comparison tests, the minimum inhibitory concentration of Cu is as high as 400ing/L, and under the condition that the soil is polluted by high-concentration cadmium, the heavy metal-resistant bacillus megaterium H3 and the new rhizobium T1_17 strain can reduce 79-96% of water-soluble cadmium and can increase the rice yield.
In the invention, a plurality of pulleys 11 are matched to facilitate the movement of the repairing device, a plurality of telescopic supporting seats 12 are matched to facilitate the fixed placement of the repairing device, one or more of arbuscular mycorrhizal fungi, trichoderma fungi, yeasts and functional bacteria and a culture medium are added into a mixing box 6, a driving motor 8 is turned on to drive a rotating shaft 9 and fan blades 10 to rotate, so that one or more of arbuscular mycorrhizal fungi, trichoderma fungi, yeasts and functional bacteria and the culture medium are fully cultured after being mixed, a first conveying pump 14 can convey a liquid repairing agent into a first conveying plate 13, the liquid repairing agent is dispersed by a dispersion net 15 and then sprayed on the polluted soil to repair the soil, a second conveying pump 19 can convey the solid repairing agent into a second conveying plate 18, and the solid repairing agent is conveyed into the polluted soil through a telescopic conveying pipe 20, the polluted soil is repaired, and the two different soil repairing means are matched, so that the soil repairing effect is improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. The utility model provides a heavy metal contaminated soil prosthetic devices, includes mount pad (1) that the level set up, its characterized in that, the downside lateral wall of mount pad (1) is equipped with the moving mechanism that two symmetries set up, the upside lateral wall of mount pad (1) is equipped with install bin (2), the upper end of install bin (2) is equipped with the first connecting hole with outside intercommunication, and the upper end of install bin (2) is equipped with case lid (3) that match the setting with first connecting hole, be equipped with mixing mechanism in install bin (2), and the both sides inner wall of install bin (2) all is equipped with limiting plate (4) that match the setting with mixing mechanism, the both sides of install bin (2) are equipped with first conveying mechanism and second conveying mechanism respectively, and first conveying mechanism and second conveying mechanism all match the setting with mixing mechanism.
2. The heavy metal contaminated soil remediation device according to claim 1, wherein the two side walls of the box cover (3) are fixedly connected with vertically arranged first insertion rods (5), and the two side walls of the installation box (2) are provided with insertion grooves matched with the first insertion rods (5).
3. The heavy metal contaminated soil remediation device of claim 1, wherein the mixing mechanism comprises a mixing box (6), a second communication hole communicated with the outside is formed in the upper end of the mixing box (6), a sealing cover (7) matched with the second communication hole is formed in the upper end of the mixing box (6), a driving motor (8) is arranged on the box cover (3), the output end of the driving motor (8) sequentially penetrates through the side walls of the box cover (3) and the sealing cover (7) and is movably clamped with a rotating shaft (9), and a plurality of fan blades (10) are arranged on two sides of the rotating shaft (9).
4. The heavy metal contaminated soil remediation device according to claim 1, wherein the moving mechanism comprises two symmetrically arranged pulleys (11), and the side walls of the two pulleys (11) opposite to each other are provided with telescopic supporting seats (12) matched with the pulleys (11).
5. The heavy metal contaminated soil remediation device of claim 3, the first conveying mechanism comprises a first conveying plate (13), the first conveying plate (13) is of a hollow structure, and the upper end of the first conveying plate (13) is provided with a first conveying pump (14), the input end of the first conveying pump (14) sequentially penetrates through the side walls of the mounting box (2) and the mixing box (6), and the output end of the first conveying pump (14) is communicated with the interior of the first conveying plate (13), the lower side wall of the first conveying plate (13) is provided with a third communication hole communicated with the outside, a dispersion net (15) is arranged in the third communicating hole, second inserting rods (16) matched with the dispersion net (15) are inserted into the side walls of the two sides of the first conveying plate (13), and the side walls of the two sides of the first conveying plate (13) are provided with buckles (17) matched with the second inserted bars (16).
6. The heavy metal contaminated soil remediation device of claim 3, wherein the second conveying mechanism comprises a second conveying plate (18), the second conveying plate (18) is of a hollow structure, a second conveying pump (19) is arranged at the upper end of the second conveying plate (18), the input end of the second conveying pump (19) sequentially penetrates through the side walls of the installation box (2) and the mixing box (6), the output end of the second conveying pump (19) is communicated with the inside of the second conveying plate (18), a plurality of telescopic conveying pipes (20) are inserted into the second conveying plate (18), and the plurality of telescopic conveying pipes (20) are communicated with the inside of the second conveying plate (18).
7. The bioremediation method of heavy metal contaminated soil according to claim 1, comprising the steps of:
fungus repair:
1) arbuscular mycorrhizal fungi.
2) A fungus of the genus trichoderma.
3) A yeast.
And (4) repairing functional bacteria.
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