CN111631130A - Method for accelerating strong winter wheat hybridization breeding process by embryo culture technology for 4 generations in 1 year - Google Patents
Method for accelerating strong winter wheat hybridization breeding process by embryo culture technology for 4 generations in 1 year Download PDFInfo
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Abstract
The invention belongs to the agricultural scientific research technology, and relates to the field of crop breeding science. In particular to a method for accelerating the cross breeding process of strong winter wheat by 1 year 4 generations, which is suitable for an embryo culture technology and mainly comprises the following steps: the first step is as follows: wheat immature embryo culture technology; the second step is that: manual vernalization treatment technology for seedlings; the third step: a wheat plant reproductive growth regulation technology; the fourth step: a cross breeding progeny system is a 1 year 4 generation accelerating gene homozygous technology. The method is simple and convenient to operate, controllable in program, economical and practical, completely aims at the 1-year 4-generation accelerating gene homozygous technology of strong winter wheat by utilizing an embryo culture technology, an artificial vernalization treatment technology, a reproductive growth regulation and control technology and a hybridization breeding progeny system, greatly shortens the growth period of the strong winter wheat under the indoor culture controllable condition, accelerates the hybridization breeding process of the strong winter wheat, and realizes the 4-year strong winter wheat breeding.
Description
Technical Field
The invention belongs to the agricultural scientific research technology, and relates to the field of crop breeding science. In particular to a method suitable for accelerating the cross breeding process of strong winter wheat by 4 generations in 1 year through an embryo culture technology.
Background
The wheat embryo culture technology is a commonly used technology in wheat breeding and research at present. The technology can accelerate the breeding process of breeding and scientific research materials, and provides test materials for researching the growth and development, morphological structure, physiological and biochemical and genetic variation rules of winter wheat breeding materials and accelerating the breeding process. Therefore, the winter wheat embryo culture technology is a basic test technology for wheat breeding and scientific research.
The winter wheat embryo culture technology is a method for quickly obtaining regenerated plants and accelerating a breeding process by using embryos as explant regeneration bodies to perform tissue culture. In the past, people use anthers, young ears, apical meristems and the like as explants to perform tissue culture to obtain regenerated plants, and in recent years, a tissue culture regeneration technology using young embryos and mature embryos as explants has been developed. The prior invention technology which is closest to the method at present is phyllanthus and the like (a method for culturing wheat tissue, application (patent) No. CN 200910237362.1). Firstly, wheat embryos are induced and cultured into embryonic callus; then differentiating and culturing the embryonic callus into a wheat regeneration plant; the method for inducing culture comprises the following steps: and sequentially carrying out induction culture on the wheat embryo by using a culture medium containing auxin, carrying out induction culture by using a culture medium containing no auxin and then carrying out induction culture by using a culture medium containing the auxin to obtain the embryogenic callus. The technology invented by the bisexual engineerine does not specifically utilize an embryo culture technology to accelerate the cross breeding process of strong winter wheat, and the strong winter wheat has the characteristics of long field growth period, strong external low temperature resistance and long vernalization time, so that when the wheat is planted and cultured indoors, a plant can be crazy and tillered, and the phenomenon of spike and fruit setting cannot be caused, or even if some strong winter wheat varieties pass through the vernalization stage, the growth and development among different plants are inconsistent, so that the problem of incapability of carrying out unified test treatment can be caused.
In the existing strong winter wheat hybridization breeding process, the progeny system material generated by hybridization breeding is generally grown in the whole growth period of about 240 plus 280d in the fields of southern and northern Xinjiang wheat areas, and usually only 1 generation of field planting can be carried out in one strong winter wheat planting year, so that the breeding progeny system material gene homozygous speed is lower. The method is also the reason that the new variety is bred by the strong winter wheat hybridization breeding through a field selection method, the breeding period is longer, and the new variety can be bred only after at least 8-10 years at the fastest speed. How to shorten the long breeding period of the strong winter wheat hybridization breeding, accelerate the strong winter wheat hybridization breeding process and improve the breeding efficiency of new varieties is a problem to be solved urgently.
Disclosure of Invention
The invention aims to provide a method for accelerating the strong winter wheat hybridization breeding process by using an embryo culture technology for 1 year and 4 generations, which is simple and convenient to operate, controllable in program, economical and practical, completely aims at the strong winter wheat, and greatly shortens the growth period of the strong winter wheat under the indoor culture controllable condition by using the embryo culture technology, the artificial vernalization treatment technology, the reproductive growth regulation and control technology and the 1 year and 4 generation accelerating gene homozygous technology of a hybridization breeding progeny system, accelerates the strong winter wheat hybridization breeding process, realizes 4 generations in one year, and provides test materials for researching the growth and development, morphological structure, physiological biochemistry and genetic variation rules of the strong winter wheat breeding materials and accelerating the strong winter wheat hybridization breeding process.
The invention discloses a method for accelerating a strong winter wheat hybridization breeding process by 1 year 4 generations of embryo culture technology, which mainly comprises the following operation steps:
the first step is as follows: the wheat embryo culture technology comprises the following steps:
marking the flowering period of the wheat filial generation system material: marking the flowering period by adopting color wool, namely taking flowering of kernel in the middle of the spike on the same day as a standard for consistent flowering period of a sampling group, and binding the base of the spike shaft by using the wool with the same color for marking; selecting wheat ears with consistent flowering period 10-15 days after flowering, stripping immature seeds, storing in a refrigerator at 4 ℃ for 1-2 days, and improving the callus induction rate and differentiation rate of wheat immature embryos; the peeled seeds are disinfected and sterilized on a superclean workbench; placing the sterilized seeds in a sterile culture dish, fixing the seeds with forceps to enable the ventral sulcus of the seeds to face downwards, and picking out immature embryos with a dissecting needle; selecting complete immature embryos with the diameter of 0.8-1.2mm, placing the complete immature embryos on an aseptic 1/2MS culture medium with scutellum upwards, and uniformly distributing and inoculating 10-15 immature embryos in each culture dish; placing the culture dish inoculated with the immature embryos in a 20-25 ℃ illumination incubator or a phytotron for culture, differentiating the immature embryos after 15-20 days of culture to grow meristematic tissues, and continuing to culture the embryos to root and grow into seedlings;
the second step is that: the seedling artificial vernalization treatment technology comprises the following steps:
transplanting the wheat seedlings which are cultured on the culture medium and have good root systems into a flowerpot with the transplanting depth of 3-5cm, placing the transplanted flowerpot in an artificial climate chamber for culturing, wherein the illumination time is set to be 7-9h every 24h, the temperature is 20-25 ℃, the humidity is 55-65% for culturing until the tillering stage, the height of the plant is about 10-15cm, each plant generally keeps 2-3 robust tillers, and the rest of the young tillers are cut from the root base; transferring the strong winter wheat seedlings entering the tillering stage into an illumination incubator, and simulating the vernalization process of the strong winter wheat under the field natural condition to carry out artificial vernalization; placing the wheat with strong winter property in the tillering stage into an illumination incubator for variable temperature culture; the incubator operating mode was adjusted as follows: the duration time of the day illumination mode is 7-9h, and the temperature is kept at 5-7 ℃; the duration time of the dark mode at night is 15-17h, the temperature is kept at 2-4 ℃, and the humidity is controlled at 60-65%; watering once every 5-7d during the artificial vernalization temperature-changing culture period, shearing off redundant tillers once every 5-7d, generally keeping 2-3 robust tillers for each plant for a long time, and topdressing nitrogen-phosphorus-potassium compound fertilizer once every 12-14 d; when the plant is cultured until the internode protuberance of the plant base is elongated, the phenomenon of rising and jointing occurs, and then the culture of the artificial vernalization treatment stage can be completed;
the third step: the wheat plant reproductive growth regulation and control technology comprises the following steps:
moving the strong winter wheat seedlings which are cultured to be in the lifted state after the artificial vernalization treatment back to the indoor culture, setting the illumination time to 12-16h every 24h by using a red and blue cold light source for plant culture, culturing at the temperature of 20-25 ℃ and the humidity of 45-55%, watering the winter wheat seedlings entering the reproductive growth stage once every 2-4d at regular time, fertilizing once every 8-10d according to the growth condition of the plants, cutting off redundant tillers once every 5-7d, and keeping 2-3 robust tillers of each plant; along with the growth and development of strong winter wheat, the tillering quantity is gradually reduced after heading, and the growth speed of young redundant tillering is gradually reduced, so that the time interval for trimming redundant tillering can be prolonged, and the trimming is not carried out after flowering, until the seeds are mature;
the fourth step: 1 year 4 generation accelerating gene homozygous technology for cross breeding progeny system:
hybridizing strong winter wheat, and breeding F2Generation of seed, generation F2Planting the seeds of the selected plants in the field to generate F3Substitute system, take F of the selection2After 10-15 days after the generation strain flowers, the seeds in the middle of the ears are cultured by the embryo culture technology, the seedling artificial vernalization technology and the reproductive growth regulation technology, the whole breeding period of the high generation strain of the strong winter wheat crossbreeding is shortened from 255 plus-280 days to 95-115 days, and the fast propagation and production of the strong winter wheat within 1 year to F can be realized6And (4) generation.
Preferably, in the first step, the peeled kernels are sterilized on a clean bench by the following steps: soaking in 75% alcohol for 2-3min, and washing with sterile water; with 0.1% HgCl2Sterilizing and soaking in the solution for 8-10min, washing with sterile water for 2-3 times, and irradiating under ultraviolet lamp for 20-30 min.
In the existing strong winter wheat hybridization breeding process, the progeny system material generated by hybridization breeding is generally grown in the whole growth period of about 240 plus 280d in the fields of southern and northern Xinjiang wheat areas, and usually only 1 generation of field planting can be carried out in one strong winter wheat planting year, so that the breeding progeny system material gene homozygous speed is lower. The method is also the reason that the new variety is bred by the strong winter wheat hybridization breeding through a field selection method, the breeding period is longer, and the new variety can be bred only after at least 8-10 years at the fastest speed. How to shorten the long breeding period of the strong winter wheat hybridization breeding, accelerate the strong winter wheat hybridization breeding process and improve the breeding efficiency of new varieties. Two problems are not completely solved: firstly, the effective growth period of the strong winter wheat is not much different from the original spring wheat, the weak growth period of the strong winter wheat in the long winter of nearly 4 months is needed in the ice, snow and field, and the low temperature of about-20 to-30 ℃ is beneficial to the smooth passing of the strong winter wheat through the vernalization stage, but the time of the whole growth period is prolonged. How to shorten the invalid growth period of the strong winter wheat and enable the wheat to finish the low-temperature vernalization stage and smoothly enter the illumination stage. Secondly, the time from the flowering to the maturation of the strong winter wheat is further shortened by adopting an embryo culture technology, so that the growth period of the strong winter wheat is further shortened. The invention provides a practical and practical solution to the two problems.
By the technical scheme of the invention, the strong winter wheat cultured by the embryo culture technology can smoothly complete the whole growth period only in about 95-115 days. The method can greatly shorten the grain filling maturation period by embryo culture of strong winter wheat under indoor conditions, and can rapidly complete the whole life cycle by artificial vernalization technology and reproductive growth regulation technology of seedlings, so as to form plants with strong growth vigor and complete development of each part of organs.
The technical advantages of the invention are illustrated by the comparison table of indoor culture and strong winter wheat growth time under field conditions by the technical method provided by the invention.
TABLE 1 comparison table of growth period of strong winter wheat embryo culture technique and field natural growth
From the data in the table, the strong winter wheat stage development is from emergence to maturity, the time of artificial indoor culture is only 25.5% -32.1% of the field natural growth time, analysis of each growth stage shows that the vegetative growth stage (emergence, tillering and jointing) and the vegetative growth and reproductive growth merging stage (booting and heading) of the strong winter wheat are both shorter than the normal field growth time, especially for the stage from tillering to jointing, the time of artificial indoor culture is only 11.8% -16.7% of the field natural growth time, and the growth time is greatly shortened. In the reproductive growth stage, the ear is taken to the mature stage, and the young embryo culture technology is adopted, so that the seeds do not need to be completely developed and mature, the young embryos 10-15 days after the flowers are stripped are cultured and planted for the next generation by using the technical method, and the grain filling mature stage is greatly shortened compared with the normal strong winter wheat mature in the field.
The effect of applying the technical method to the hybrid breeding progeny system in accelerating the breeding process is obvious, as shown in Table 2, the acceleration of the hybrid breeding process by utilizing the embryo culture technology is compared with the normal hybrid breeding process, and the result shows that F is the ratio of the yield of the hybrid breeding to the yield of the normal hybrid breeding0-F10The generation can be realized in 11 years under normal conditions, the embryo culture technology only needs 5 years, the time of the technical method is equal to 45.5% of that of normal field growth, and the crossbreeding process of the strong winter wheat can be greatly improved. Therefore, the method organically combines the immature embryo culture technology, the seedling artificial vernalization treatment technology, the plant reproductive growth regulation and control technology and the 1-year 4-generation accelerated homozygous technology of the cross breeding progeny system, accelerates the conventional cross breeding process of the strong winter wheat, and powerfully promotes the rapid breeding of new varieties.
TABLE 2 comparison table of accelerated cross breeding process and normal cross breeding process for strong winter wheat embryo culture
The method provided by the invention is simple and convenient to operate, controllable in program, economical and practical, completely aims at the 1-year 4-generation accelerating gene homozygous technology of the strong winter wheat utilization embryo culture technology, the artificial vernalization treatment technology, the reproductive growth regulation technology and the hybridization breeding progeny system, greatly shortens the growth period of the strong winter wheat under the indoor culture controllable condition, accelerates the strong winter wheat hybridization breeding process, realizes the one-year 4-generation breeding, and can provide test materials for researching the growth and development, morphological structure, physiological biochemistry and genetic variation rules of the strong winter wheat breeding material and accelerating the strong winter wheat hybridization breeding process.
Detailed Description
The specific implementation scheme of the technology is explained in detail by taking strong winter wheat as an example below:
1. and (5) preparing wheat immature embryo culture. A commercially available 1/2MS culture medium is used as a substrate for culturing the meristematic tissue of the immature embryo, and the immature embryo is sterilized at high temperature and subpackaged in a sterile culture dish for later use. Selecting wheat ears with consistent flowering phase marks, stripping wheat grains 10-15d after flowering in the middle of the wheat ears, storing the wheat grains in a refrigerator at 4 ℃ for 1-2d, and then sterilizing on a superclean workbench: soaking in 75% ethanol for 2-3min, and washing with sterile water. With 0.1% HgCl2Sterilizing and soaking in the solution for 8-10min, washing with sterile water for 2-3 times, and irradiating under ultraviolet lamp for 20-30 min.
2. Picking and inoculating and culturing wheat immature embryos. The sterilized seeds are placed in a sterile culture dish, the seeds are fixed by forceps with the ventral sulcus facing downwards, and the young embryos are picked out by a dissecting needle. Whole immature embryos with the diameter of 0.8-1.2mm are selected, the scutellum is upwards placed on a sterile 1/2MS culture medium, and 10-15 immature embryos are evenly distributed and inoculated in each culture dish. Culturing in 25 deg.C illumination incubator (artificial climate chamber), culturing for 15-20 days, differentiating to grow meristem, and further culturing to obtain seedling.
3. And (5) related preparation work before transplanting seedlings. The method comprises selecting commercially available sterilized culture soil mixed with perlite (culture soil: perlite in a ratio of 3: 1) as indoor culture medium for wheat with strong winter property, selecting plastic flowerpot with height of 20-25cm and diameter of pot mouth of 15-18cm, and matching with corresponding size of base tray. Before sowing, firstly, placing the flowerpot on a tray, then laying a layer of perlite with the thickness of 1-2cm on the bottom of the flowerpot, then placing a culture medium which is prepared in advance and uniformly mixed into the flowerpot, filling the flowerpot and compacting the flowerpot by using force.
4. And (5) transplanting seedlings and culturing and managing. Transplanting the cultured wheat seedlings with the plant height of 3-5cm and good root system development into plastic flowerpots, transplanting 8-10 wheat seedlings in each pot, transplanting the seedlings with the transplanting depth of 3-5cm, culturing the transplanted flowerpots in an artificial climate chamber under the condition that the illumination time is 7-9h (every 24h), the temperature is 20-25 ℃, the humidity is 55-65% until the tillering stage is reached, the plant height is about 10-15cm, each plant generally keeps 2-3 robust tillers, and the rest young tillers are cut from the root base.
5. Artificial vernalization of wheat. Putting the winter wheat seedlings which enter the tillering stage into an SPX-250IC type illumination incubator for culture, and adjusting the operation mode of the incubator as follows: the duration time of the daytime mode is 7-9h, and the temperature is 5-7 ℃; the duration of the night mode is 15-17h, and the temperature is kept at 2-4 ℃. Watering every 5-7 days during the culture period, cutting off redundant tillers every 5-7 days (each plant keeps 2-3 strong tillers), applying nitrogen-phosphorus-potassium compound fertilizer every 12-14 days, continuously culturing at variable temperature for 20-30 days, and checking whether winter wheat seedlings grow up and stem, wherein if the winter wheat seedlings grow up and stem, the vernalization stage of the strong winter wheat is completed. The rising joint checking method comprises the following steps: pinching the base part of the tillering of the wheat seedlings by hands, carefully touching to feel whether the internode bulges exist, if so, indicating that the vernalization stage of the strong winter wheat is completed, rising and jointing, and starting to enter the illumination stage.
6. Culturing strong winter wheat in the reproductive growth stage. And transferring the strong winter wheat seedlings which have completed the vernalization stage to an indoor culture room for culture. Before transferring into a culture room, redundant tillering and dead leaves of each wheat seedling are cut off, and only 2-3 tillering which grow strongly and are already knotted are reserved for each wheat seedling. Culturing the trimmed wheat seedlings in an environment with the light condition of 12-16h, the temperature of 20-25 ℃, the humidity of 45-55%, the dark condition of 8-12h, the temperature of 20-25 ℃ and the humidity of 45-55%, watering once a day, applying the nitrogen-phosphorus-potassium compound fertilizer once every 12-14d according to the growth condition, and trimming ineffective tillers once every 7-10 d. The tillering quantity of the plant is gradually reduced along with the growth and development of the wheat from a vegetative growth stage to a reproductive growth stage, the growth speed of the plant is gradually reduced, the time interval of tillering pruning can be properly prolonged, and the plant can not be pruned after the plant enters an ear-sprouting flowering stage until seeds are mature.
7. After strong winter wheat seedlings enter the booting stage, the fertilization amount needs to be increased properly and the fertilization interval time needs to be shortened properly due to the fact that winter wheat enters the key period of fertilizer demand, and nitrogen-phosphorus-potassium compound fertilizers are applied once every 8-10d according to the growth situation of plants. The fertilizer proportioning and application method comprises the following steps: a nitrogen-phosphorus-potassium compound fertilizer suspension with the mass fraction of 30% is prepared in advance, is shaken up before use each time, and is watered with 10ml of fertilizer per pot and 300ml-500ml of water for watering. The watering method comprises the following steps: 300ml-500ml per pot each time. Meanwhile, the culture room is also required to prevent insects and diseases, so that a good growth environment is created for the growth of winter wheat. The fertilization interval time in the later grain filling period is gradually prolonged, so that the greedy of plants is prevented, and the grain maturity time is prolonged.
8. In the filial generation of strong winter wheat, from F2Selecting strains in generations, marking florescence, and selecting selected F according to the above-mentioned 1-7 steps2After 10-15 days after the generation line flowers, the seeds in the middle of the ears are cultured by the embryo culture technology, the seedling artificial vernalization technology and the reproductive growth regulation technology, the whole breeding period of the high generation line of the strong winter wheat crossbreeding is shortened from 255 plus-280 days to 65-90 days, and the fast propagation and production of the strong winter wheat within 1 year to F can be realized6Instead, F is7Planting the generation seeds in the field, then carrying out field selection of high generation strains for 1-2 years, and finally breeding the new wheat variety with strong winter property by taking the selected strains into strain identification, variety comparison, area test and production test.
Claims (2)
1. A method for accelerating the cross breeding process of strong winter wheat by 1 year and 4 generations through an embryo culture technology is characterized by mainly comprising the following operation steps:
the first step is as follows: the wheat embryo culture technology comprises the following steps:
marking the flowering period of the wheat filial generation system material: marking the flowering period by adopting color wool, namely taking flowering of kernel in the middle of the spike on the same day as a standard for consistent flowering period of a sampling group, and binding the base of the spike shaft by using the wool with the same color for marking; selecting wheat ears with consistent flowering period 10-15 days after flowering, stripping immature seeds, storing in a refrigerator at 4 ℃ for 1-2 days, and improving the callus induction rate and differentiation rate of wheat immature embryos; the peeled seeds are disinfected and sterilized on a superclean workbench; placing the sterilized seeds in a sterile culture dish, fixing the seeds with forceps to enable the ventral sulcus of the seeds to face downwards, and picking out immature embryos with a dissecting needle; selecting complete immature embryos with the diameter of 0.8-1.2mm, placing the complete immature embryos on an aseptic 1/2MS culture medium with scutellum upwards, and uniformly distributing and inoculating 10-15 immature embryos in each culture dish; placing the culture dish inoculated with the immature embryos in a 20-25 ℃ illumination incubator or a phytotron for culture, differentiating the immature embryos after 15-20 days of culture to grow meristematic tissues, and continuing to culture the embryos to root and grow into seedlings;
the second step is that: the seedling artificial vernalization treatment technology comprises the following steps:
transplanting the wheat seedlings which are cultured on the culture medium and have good root systems into a flowerpot with the transplanting depth of 3-5cm, placing the transplanted flowerpot in an artificial climate chamber for culturing, wherein the illumination time is set to be 7-9h every 24h, the temperature is 20-25 ℃, the humidity is 55-65% for culturing until the tillering stage, the height of the plant is about 10-15cm, each plant generally keeps 2-3 robust tillers, and the rest of the young tillers are cut from the root base; transferring the strong winter wheat seedlings entering the tillering stage into an illumination incubator, and simulating the vernalization process of the strong winter wheat under the field natural condition to carry out artificial vernalization; placing the wheat with strong winter property in the tillering stage into an illumination incubator for variable temperature culture; the incubator operating mode was adjusted as follows: the duration time of the day illumination mode is 7-9h, and the temperature is kept at 5-7 ℃; the duration time of the dark mode at night is 15-17h, the temperature is kept at 2-4 ℃, and the humidity is controlled at 60-65%; watering once every 5-7d during the artificial vernalization temperature-changing culture period, shearing off redundant tillers once every 5-7d, generally keeping 2-3 robust tillers for each plant for a long time, and topdressing nitrogen-phosphorus-potassium compound fertilizer once every 12-14 d; when the plant is cultured until the internode protuberance of the plant base is elongated, the phenomenon of rising and jointing occurs, and then the culture of the artificial vernalization treatment stage can be completed;
the third step: the wheat plant reproductive growth regulation and control technology comprises the following steps:
moving the strong winter wheat seedlings which are cultured to be in the lifted state after the artificial vernalization treatment back to the indoor culture, setting the illumination time to 12-16h every 24h by using a red and blue cold light source for plant culture, culturing at the temperature of 20-25 ℃ and the humidity of 45-55%, watering the winter wheat seedlings entering the reproductive growth stage once every 2-4d at regular time, fertilizing once every 8-10d according to the growth condition of the plants, cutting off redundant tillers once every 5-7d, and keeping 2-3 robust tillers of each plant; along with the growth and development of strong winter wheat, the tillering quantity is gradually reduced after heading, and the growth speed of young redundant tillering is gradually reduced, so that the time interval for trimming redundant tillering can be prolonged, and the trimming is not carried out after flowering, until the seeds are mature;
the fourth step: 1 year 4 generation accelerating gene homozygous technology for cross breeding progeny system:
hybridizing strong winter wheat, and breeding F2Generation of seed, generation F2Planting the seeds of the selected plants in the field to generate F3Substitute system, take F of the selection2After 10-15 days after the generation strain flowers, the seeds in the middle of the ears are cultured by the embryo culture technology, the seedling artificial vernalization technology and the reproductive growth regulation technology, the whole breeding period of the high generation strain of the strong winter wheat crossbreeding is shortened from 255 plus-280 days to 95-115 days, and the fast propagation and production of the strong winter wheat within 1 year to F can be realized6And (4) generation.
2. The method for accelerating the cross breeding process of strong winter wheat by 1 year 4 generations of embryo culture technology as claimed in claim 1, wherein in the first step, the peeled kernels are sterilized on a clean bench by the following steps: soaking in 75% alcohol for 2-3min, and washing with sterile water; with 0.1% HgCl2Sterilizing and soaking in the solution for 8-10min, washing with sterile water for 2-3 times, and irradiating under ultraviolet lamp for 20-30 min.
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| CN111631131A (en) * | 2020-06-09 | 2020-09-08 | 石河子大学 | Method for accelerating strong winter wheat hybridization breeding process in 3 generations in 1 year |
| CN112400691A (en) * | 2020-11-17 | 2021-02-26 | 甘肃省农业科学院小麦研究所 | Culture medium suitable for crop immature embryo culture and application of culture medium in rapid breeding of immature embryo one-step seedling |
| CN112704005A (en) * | 2020-12-29 | 2021-04-27 | 石河子大学 | Rapid directional improvement technology for wheat target character |
| CN113748949A (en) * | 2021-09-15 | 2021-12-07 | 浙江省亚热带作物研究所 | Dianthus superbus standardized propagation production method |
| CN114041418A (en) * | 2021-12-15 | 2022-02-15 | 襄阳市农业科学院 | Method for producing wheat double haploid by distant hybridization of corn and wheat |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262031A (en) * | 1999-02-01 | 2000-08-09 | 河北省农林科学院农业物理生理生化研究所 | High-speed plant breeding technique by more generations in one year |
| CN101828521A (en) * | 2010-05-18 | 2010-09-15 | 江苏省农业科学院 | Method for quick transformation of major QTL with resistance to fusarium head blight through combination of embryo culture of wheat and sign selection |
| CN103004450A (en) * | 2013-01-15 | 2013-04-03 | 山东农业大学 | Novel method of using land and greenhouse for anniversary cultivation of fourth-generation winter wheat |
| CN103026970A (en) * | 2013-01-15 | 2013-04-10 | 山东农业大学 | Novel method for transplanting winter wheat young embryo one-step seedlings big summer fields for fast growing |
| CN103540608A (en) * | 2013-10-08 | 2014-01-29 | 江苏省农业科学院 | Method for rapidly obtaining transgenic wheat homozygous plant |
| CN105706694A (en) * | 2016-02-25 | 2016-06-29 | 石河子大学 | Indoor cultivation method for wheat with strong winterness |
| CN111631131A (en) * | 2020-06-09 | 2020-09-08 | 石河子大学 | Method for accelerating strong winter wheat hybridization breeding process in 3 generations in 1 year |
-
2020
- 2020-06-09 CN CN202010520244.8A patent/CN111631130A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262031A (en) * | 1999-02-01 | 2000-08-09 | 河北省农林科学院农业物理生理生化研究所 | High-speed plant breeding technique by more generations in one year |
| CN101828521A (en) * | 2010-05-18 | 2010-09-15 | 江苏省农业科学院 | Method for quick transformation of major QTL with resistance to fusarium head blight through combination of embryo culture of wheat and sign selection |
| CN103004450A (en) * | 2013-01-15 | 2013-04-03 | 山东农业大学 | Novel method of using land and greenhouse for anniversary cultivation of fourth-generation winter wheat |
| CN103026970A (en) * | 2013-01-15 | 2013-04-10 | 山东农业大学 | Novel method for transplanting winter wheat young embryo one-step seedlings big summer fields for fast growing |
| CN103540608A (en) * | 2013-10-08 | 2014-01-29 | 江苏省农业科学院 | Method for rapidly obtaining transgenic wheat homozygous plant |
| CN105706694A (en) * | 2016-02-25 | 2016-06-29 | 石河子大学 | Indoor cultivation method for wheat with strong winterness |
| CN111631131A (en) * | 2020-06-09 | 2020-09-08 | 石河子大学 | Method for accelerating strong winter wheat hybridization breeding process in 3 generations in 1 year |
Non-Patent Citations (1)
| Title |
|---|
| 安海龙等: "小麦幼胚培养高效成株系统的建立", 《植物生理学报》 * |
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|---|---|---|---|---|
| CN111631131A (en) * | 2020-06-09 | 2020-09-08 | 石河子大学 | Method for accelerating strong winter wheat hybridization breeding process in 3 generations in 1 year |
| CN112400691A (en) * | 2020-11-17 | 2021-02-26 | 甘肃省农业科学院小麦研究所 | Culture medium suitable for crop immature embryo culture and application of culture medium in rapid breeding of immature embryo one-step seedling |
| CN112704005A (en) * | 2020-12-29 | 2021-04-27 | 石河子大学 | Rapid directional improvement technology for wheat target character |
| CN113748949A (en) * | 2021-09-15 | 2021-12-07 | 浙江省亚热带作物研究所 | Dianthus superbus standardized propagation production method |
| CN114041418A (en) * | 2021-12-15 | 2022-02-15 | 襄阳市农业科学院 | Method for producing wheat double haploid by distant hybridization of corn and wheat |
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