CN111615978A - Calculation method for application amount of tolfenpyrad in tea garden for preventing and treating tea lesser leafhoppers based on electrostatic spraying - Google Patents
Calculation method for application amount of tolfenpyrad in tea garden for preventing and treating tea lesser leafhoppers based on electrostatic spraying Download PDFInfo
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- WPALTCMYPARVNV-UHFFFAOYSA-N tolfenpyrad Chemical compound CCC1=NN(C)C(C(=O)NCC=2C=CC(OC=3C=CC(C)=CC=3)=CC=2)=C1Cl WPALTCMYPARVNV-UHFFFAOYSA-N 0.000 title claims abstract description 164
- 241001414720 Cicadellidae Species 0.000 title claims abstract description 48
- 238000007590 electrostatic spraying Methods 0.000 title claims abstract description 20
- 241001122767 Theaceae Species 0.000 title claims abstract 31
- 238000004364 calculation method Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 24
- 230000000694 effects Effects 0.000 claims abstract description 9
- 230000000361 pesticidal effect Effects 0.000 claims abstract description 8
- 239000003814 drug Substances 0.000 claims abstract description 6
- 229940079593 drug Drugs 0.000 claims abstract description 5
- 230000002265 prevention Effects 0.000 claims abstract description 5
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims description 41
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- 235000015459 Lycium barbarum Nutrition 0.000 claims description 41
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- 239000000575 pesticide Substances 0.000 claims description 18
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- 230000029087 digestion Effects 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 7
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- 244000269722 Thea sinensis Species 0.000 description 66
- 241000255789 Bombyx mori Species 0.000 description 3
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- OMFRMAHOUUJSGP-IRHGGOMRSA-N bifenthrin Chemical compound C1=CC=C(C=2C=CC=CC=2)C(C)=C1COC(=O)[C@@H]1[C@H](\C=C(/Cl)C(F)(F)F)C1(C)C OMFRMAHOUUJSGP-IRHGGOMRSA-N 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- VBCVPMMZEGZULK-NRFANRHFSA-N indoxacarb Chemical compound C([C@@]1(OC2)C(=O)OC)C3=CC(Cl)=CC=C3C1=NN2C(=O)N(C(=O)OC)C1=CC=C(OC(F)(F)F)C=C1 VBCVPMMZEGZULK-NRFANRHFSA-N 0.000 description 2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a method for calculating the application amount of tolfenpyrad in a tea garden based on electrostatic spraying for preventing and treating tea lesser leafhoppers, which comprises the following steps: determining the maximum using amount of tolfenpyrad in the tea garden according to the safe drug application threshold value, the safe environment threshold value and the MRL value of tolfenpyrad registered in the tea; and calculating the minimum effective dose of the tolfenpyrad in the tea garden according to the prevention effect of the tolfenpyrad on the tea lesser leafhoppers, residual tolfenpyrad data and the resistance occurrence rule of the tea lesser leafhoppers. The application amount of tolfenpyrad electrostatic spraying is determined according to the invention, so that the pesticide effect and the safety can be ensured.
Description
Technical Field
The invention relates to a calculation method for the application amount of tolfenpyrad in a tea garden for preventing and treating tea lesser leafhoppers based on electrostatic spraying, and belongs to the technical field of pesticide preparations.
Background
The main pest of the tea garden is tea lesser leafhopper, the prevention and control difficulty is high, and the application limit standard and standard pesticide application is not determined at present. The existing pesticide varieties registered on tea leaves for preventing and controlling the tea lesser leafhoppers mainly comprise tolfenpyrad, indoxacarb and bifenthrin, the indoxacarb has relatively low prevention effect, the bifenthrin is a relatively old pesticide variety, and the tolfenpyrad is a novel pyrazole heterocyclic water-insoluble pesticide, has a good contact killing effect on the tea lesser leafhoppers, and has the characteristics of broad spectrum, high efficiency, low residue and the like.
For the application of tolfenpyrad, besides the traditional manual or electric spraying, electrostatic spraying is also available at present, and tolfenpyrad is used in combination with an electrostatic sprayer, so that the pesticide utilization rate can be improved and the pesticide residue of tea leaves can be reduced under the condition of reducing the pesticide use by 20%. However, how to determine the safe and effective application amount of the tolfenpyrad electrostatic spray is a technical problem to be solved in the process of controlling tea lesser leafhoppers at present.
Disclosure of Invention
Based on the above, the invention provides a method for calculating the application amount of tolfenpyrad in a tea garden based on electrostatic spraying for preventing and treating tea lesser leafhoppers, so that the application amount of tolfenpyrad electrostatic spraying is determined, the pesticide effect can be ensured, the safety can be ensured, and the defects in the prior art can be overcome.
The technical scheme of the invention is as follows: a method for calculating the application amount of tolfenpyrad in a tea garden for preventing and treating tea lesser leafhoppers based on electrostatic spraying comprises the following steps:
determining the maximum using amount of tolfenpyrad in the tea garden according to the safe drug application threshold value, the safe environment threshold value and the MRL value of tolfenpyrad registered in the tea;
and calculating the minimum effective dose of the tolfenpyrad in the tea garden according to the prevention effect of the tolfenpyrad on the tea lesser leafhoppers, residual tolfenpyrad data and the resistance occurrence rule of the tea lesser leafhoppers.
Optionally, the food safety maximum allowable application rate of tolfenpyrad is 112.5g a.i./hm2The environmental safety maximum allowable application rate of tolfenpyrad is 112.5g a.i./hm2MRL value is 50mg/kg, and the maximum using amount of tolfenpyrad in the tea garden is 112.5g a.i./hm2;
The lowest effective dose of tolfenpyrad in tea garden is 72g a.i./hm2。
Optionally, the method for measuring the tolfenpyrad administration safety threshold comprises the following steps: according to the tolfenpyrad use label, the behavior rule of tolfenpyrad in the electrostatic spray application mode on the tea trees is researched, the deposition rule, the digestion rule, the metabolism rule and the relation between the dosage and the safe transmission of tolfenpyrad in the tea trees are determined, and accordingly the maximum allowable application dosage of tolfenpyrad is determined.
Optionally, the deposition rule of tolfenpyrad in the tea trees is that tolfenpyrad is distributed in the tea trees in an inverted pyramid shape, the deposition amount is reduced from top to bottom, and the deposition concentration of leaves is far greater than that of soil;
the digestion rule of tolfenpyrad in tea trees is that the half-life period of tolfenpyrad in a tea tree system is 1.96-3.67 d;
the metabolic rule of tolfenpyrad in tea trees is that no high-toxicity metabolite is generated;
according to the quantity value transfer relationship between tolfenpyrad application dosage and food safety, the maximum allowable tolfenpyrad application dosage of the food safety tolfenpyrad is calculated to be 112.5g a.i./hm2;
The maximum application rate of tolfenpyrad is 112.5g a.i./hm determined according to the above2。
Optionally, calculating the least significantDuring dosage, according to different pesticide application dosages of tolfenpyrad, tolfenpyrad is sprayed by an electrostatic sprayer to prevent and control tea lesser leafhoppers, the population density of the tea lesser leafhoppers is investigated by adopting a five-point sampling method to obtain the pesticide application amount and pesticide effect of the tolfenpyrad, and the half-life period of the tolfenpyrad in a tea tree system is calculated by pesticide residue data and a kinetic equation; calculating LD of tolfenpyrad on tea lesser leafhopper by tea leaf soaking method test50And LD95。
Optionally, the determination method of the tolfenpyrad environmental safety threshold comprises the following steps: according to NY/T2882 'guidelines for evaluating environmental risk of pesticide registration', the allowable concentration value of pesticide residue in the environment is pushed back based on an environmental risk model aiming at tea leafhoppers in a tea tree scene, the pesticide application amount is pushed back according to the standard that the pesticide residue in the environment cannot exceed the risk value of non-target organisms at any moment, and the lowest effective dose of tolfenpyrad is 72g a.i./hm2And a maximum registered dose of 112.5ga.i./hm2Calculating that the maximum using dose of tolfenpyrad should be less than 112.5g a.i./hm2。
The invention has the beneficial effects that: aiming at the main pests of the current tea garden, namely tea lesser leafhoppers, and the aspects of comprehensive control effect, pesticide residue and the like, candidate medicaments tolfenpyrad and electrostatic spray application equipment are screened out, and the application limiting quantity value range of tolfenpyrad is pushed back to 72-112.5g a.i./hm according to the comprehensive factors of the application safety threshold value (the deposition rule, the digestion rule, the metabolic rule and the safe transmission relation of the dose and the safe transmission relation of tolfenpyrad in tea trees) of tolfenpyrad, the lowest effective dose of tolfenpyrad, environmental safety and the like after electrostatic spray application of tolfenpyrad2Definitely, under the condition that the tolfenpyrad is reduced by 20 percent and improved by 12 percent, the electrostatic spray application limit standard range of the tea tolfenpyrad is established to be 72-112.5g a.i./hm2And important technical support is provided for the decrement use of tolfenpyrad in a tea planting system.
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FIG. 1 shows that the tolfenpyrad deposition amount is distributed in the shape of an inverted pyramid on the tea plant.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed as broadly as the present invention is capable of modification in various respects, all without departing from the spirit and scope of the present invention.
The embodiment of the invention provides a method for calculating the application amount of tolfenpyrad in a tea garden for preventing and treating tea lesser leafhoppers based on electrostatic spraying, which comprises the following steps:
firstly, determining the maximum using amount of tolfenpyrad in a tea garden according to a drug application safety threshold value, an environment safety threshold value and the MRL value of tolfenpyrad registered in tea leaves.
Tolfenpyrad safety threshold for administration
Using a label (67.5-112.5g a.i./hm) according to tolfenpyrad2) The method is used for researching the behavior rule of tolfenpyrad in the electrostatic spraying mode on the tea trees and determining the deposition rule, the digestion rule, the metabolism rule and the dose and safe transfer relation of tolfenpyrad in the tea trees.
1. Deposition rule of tolfenpyrad in tea tree
In terms of the deposition behavior of tolfenpyrad, the application rate of tolfenpyrad by an electrostatic spray applicator is 72ga.i./hm2In the process, after the pesticide is applied for 2 hours, tolfenpyrad is mainly deposited in leaves and soil, and the upper plant of the tea tree accounts for 84.6-96.2% of the whole plant. The deposition sequence of tolfenpyrad in the tea tree-soil system is as follows: 57.6-63.6% of the sediment is deposited on the upper leaves of the tea trees, 27-32.6% of the sediment is deposited on the lower leaves, and 2.6-15.3% of the sediment is deposited on the soil part. Tolfenpyrad is distributed in tea tree in inverted pyramid form, deposition amount decreases from top to bottom, and leaf deposition concentration is far greater than that of soil, and is closely related to growth structure of tea tree (figure 1 and table 1)
Table 1: tolfenpyrad sedimentary spectrum in tea tree
2. Rule of tolfenpyrad in tea tree
According to different application doses of tolfenpyrad (36,72,108,112.5g a.i./hm)2) Measuring pesticide residue values in different time periods (2h,1d,3d,5d and 7d), calculating the half-life period of tolfenpyrad in the tea tree system of China to be 1.96-3.67d through a kinetic equation, wherein the half-life period is mainly related to the application dosage and the growth environment of tea trees (Table 2)
Table 2: the digestion rule of tolfenpyrad in tea (upper leaf)
3. Metabolism rule of tolfenpyrad in tea tree
Tolfenpyrad hydrolysis half-life DT in pure product50All are more than 1 year; the photolysis half-life period in water is 25 ℃, 765W/m2300-800 nm, DT in distilled water5035.2h, DT in Natural Water50Is 35 h. According to the literature reports [4-7 ]]The metabolic pathway of tolfenpyrad is photolysis reaction and hydrolysis reaction, and the metabolic product is 4- [4- [ (4-chloro-3-ethyl-1-methylpyrazole) carbonyl aminomethyl]Phenoxy radical]Benzoic acid, without the production of highly toxic metabolites.
4. Magnitude transmission relation between tolfenpyrad administration dosage and food safety
Based on parameters such as safe interval period and degradation constant of tolfenpyrad, a tea tree sprayer application scene in China is adopted, the applied crops are tea trees, the application period is the tea period in summer and autumn, the application times are 1, the application device is an electrostatic sprayer, the application interval period is 7d, the application safe interval period is 7d, the degradation half-life period is 3d, the national food safety standard registered in tea combined with tolfenpyrad is 50mg/kg (GB 2763-2) The final tolfenpyrad residues of 2h and 7d were found to be 25.6 and 4.01mg/kg, indicating that the field test values (25.6 and 4.01mg/kg) of tolfenpyrad residues are within the MRL values of tolfenpyrad registered in tea (china:50 mg/kg; CAC: 30mg/kg) to determine the safe maximum allowable dose of tolfenpyrad in the chinese and international commission on food code commission (CAC) for food products of 112.5g a.i./hm2。
5. Tolfenpyrad administration dosage on crop safety
According to different application doses of tolfenpyrad (36,72,108,112.5g a.i./hm)2) The tolfenpyrad is sprayed by the electrostatic sprayer to prevent and control tea leafhoppers, and the phenomenon of tea phytotoxicity is not found when the conditions of the tea phytotoxicity in different time periods (2h,1d,3d,5d and 7d) are investigated, so that when the application dosage is 36,72,108,112.5g of a.i./hm2In time, tolfenpyrad does not affect the normal growth of tea trees and is safe to the tea trees.
(II) environmental safety threshold
According to NY/T2882 'environmental risk assessment guidelines for pesticide registration', for tea leafhoppers in a tea tree scene, a pesticide residue allowable concentration value in an environment is pushed back based on an environmental risk model, and a pesticide application amount is pushed back according to the standard that the pesticide residue in the environment cannot exceed a risk value existing for a non-target organism at any moment (the highest value is an original deposition amount). Minimum effective dose of 72g a.i./hm combined with tolfenpyrad2And a maximum registered dose of 112.5g a.i./hm2The degradation half-life period corresponding to the application period is 3.67d, the maximum recommended application times is 1 time, and the application interval period is 7 d. Verified by field test at maximum dose (112.5g a.i./hm)2) The original deposition amount of tolfenpyrad for 2h is 25.6mg/kg (not exceeding MRL value registered in tea for CAC tolfenpyrad in China), and 30% suspending agent of tolfenpyrad product in market is medium toxic pesticide, and is toxic to silkworm, fish and bee (at 96h, tolfenpyrad 15% missible oil is toxic to zebra fish LC500.002mg a.i./L, and acute exposure of bees to LD at 48h501.33 microgram a.i./bee, LC of the method that silkworm eats the detoxified leaves at 96h505.9mg/kg mulberry leaf). Thereby determining that the maximum applied dose of tolfenpyrad should not exceed the maximum registered dose of 112.5g a.i./hm2And the flowering period of the honey crops is forbidden, the parts near the silkworm rearing room and the mulberry field are forbidden, and the medicines are taken far away from the aquaculture area.
Combine the above-mentioned implementsDrug safety threshold value nuclear environment safety threshold value, the maximum using amount of tolfenpyrad in the tea garden is determined to be 112.5g a.i./hm2。
And secondly, calculating the minimum effective dose of the tolfenpyrad in the tea garden according to the control effect of the tolfenpyrad on the tea lesser leafhoppers, residual tolfenpyrad data and the resistance occurrence rule of the tea lesser leafhoppers.
According to different application doses of tolfenpyrad (36,72,108,112.5g a.i./hm)2) Tolfenpyrad is sprayed by an electrostatic sprayer to prevent and control the tea lesser leafhoppers, and the insect population density of the tea lesser leafhoppers is investigated by adopting a five-point sampling method. The result shows that when the application amount of tolfenpyrad is 36g a.i./hm2When the composition is used, the pesticide effect of preventing and treating the tea lesser leafhopper 7d is 55.7-71.2%; when the application amount of tolfenpyrad is 72g a.i./hm2The pesticide effect for preventing and treating the tea lesser leafhopper 7d is 85.2-91%; when the application amount of tolfenpyrad is 108g a.i./hm2The pesticide effect for preventing and treating the tea lesser leafhopper 7d is 86.1-92%. The half-life period of tolfenpyrad in the tea tree system of China is calculated to be 1.96d (36g a.i./hm) through pesticide residue data and a kinetic equation2),2.08d(72g a.i./hm2),3.09d(108g a.i./hm2) And 3.67d (112.5g a.i./hm)2). Calculating LD of tolfenpyrad on tea lesser leafhopper by tea leaf soaking method test50And LD950.48 and 5.94. mu.g/mL, respectively. Therefore, according to the control effect of tolfenpyrad on tea lesser leafhoppers, tolfenpyrad residual data and the resistance occurrence rule of the tea lesser leafhoppers, when the application dosage of tolfenpyrad is 72ga.i./hm2The pesticide effect for preventing and treating the tea lesser leafhopper 7d is 85.2-91%. When the control effect of the pesticide on the tea lesser leafhoppers is more than 85 percent, the tea lesser leafhoppers can be effectively controlled. The lowest effective dose of tolfenpyrad is 72g a.i./hm2And is 1 to 1.5 times the registered minimum recommended dose (Table 3).
Table 3: tolfenpyrad in tea tree at minimum effective dose
Remarking: x represents the registered minimum recommended dose
The deposition amount of tolfenpyrad in tea tree is reduced from leaf to soil in turn, the half-life period of tolfenpyrad in tea tree system of China is 1.96-3.67d, and the maximum allowable application amount of tolfenpyrad in food safety is 112.5g a.i./hm2The lowest effective dose of tolfenpyrad is 72g a.i./hm2The environmental safety maximum allowable application rate of tolfenpyrad is 112.5ga.i./hm2Calculating the application limit value range of the tolfenpyrad to be 72-112.5g a.i./hm2And verified by field test (Table 4)
Table 4: tolfenpyrad tea garden use limit standard
At present, main pests in tea gardens are tea lesser leafhoppers, candidate agents tolfenpyrad and electrostatic spraying application instruments are screened out in the aspects of comprehensive control effect, pesticide residue and the like, and the application limiting quantity value range of tolfenpyrad is pushed back to 72-112.5g a.i./hm2 (table 4) according to comprehensive factors such as the application safety threshold value (deposition rule, digestion rule, metabolic rule and dose and safety transmission relation of tolfenpyrad in tea trees), the lowest effective dose of tolfenpyrad, environmental safety and the like after electrostatic spraying application of tolfenpyrad. The electrostatic spraying application limit standard range of 72-112.5g a.i./hm2 of the tolfenpyrad is established under the conditions of reducing the tolfenpyrad by 20% and improving by 12%, and important technical support is provided for the reduction of tolfenpyrad in tea planting systems in China.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (6)
1. A method for calculating the application amount of tolfenpyrad in a tea garden for preventing and treating tea lesser leafhoppers based on electrostatic spraying is characterized by comprising the following steps:
determining the maximum using amount of tolfenpyrad in the tea garden according to the safe drug application threshold value, the safe environment threshold value and the MRL value of tolfenpyrad registered in the tea;
and calculating the minimum effective dose of the tolfenpyrad in the tea garden according to the prevention effect of the tolfenpyrad on the tea lesser leafhoppers, residual tolfenpyrad data and the resistance occurrence rule of the tea lesser leafhoppers.
2. The method for calculating the application amount of tolfenpyrad in tea garden for controlling lesser leafhoppers based on electrostatic spraying according to claim 1,
the food safety maximum allowable application rate of tolfenpyrad is 112.5g a.i./hm2The environmental safety maximum allowable application rate of tolfenpyrad is 112.5g a.i./hm2MRL value is 50mg/kg, and the maximum using amount of tolfenpyrad in the tea garden is 112.5ga.i./hm2;
The lowest effective dose of tolfenpyrad in tea garden is 72g a.i./hm2。
3. The method for calculating the application amount of tolfenpyrad in tea gardens for controlling tea lesser leafhoppers based on electrostatic spraying according to claim 2, wherein the method for measuring the safe threshold value of tolfenpyrad application comprises the following steps: according to the tolfenpyrad use label, the behavior rule of tolfenpyrad in the electrostatic spray application mode on the tea trees is researched, the deposition rule, the digestion rule, the metabolism rule and the relation between the dosage and the safe transmission of tolfenpyrad in the tea trees are determined, and accordingly the maximum allowable application dosage of tolfenpyrad is determined.
4. The method for calculating the application amount of tolfenpyrad in tea garden for controlling lesser leafhoppers based on electrostatic spraying according to claim 3,
the deposition rule of tolfenpyrad in tea trees is that tolfenpyrad is distributed in the tea trees in an inverted pyramid shape, the deposition amount is reduced from top to bottom, and the deposition concentration of leaves is far greater than that of soil;
the digestion rule of tolfenpyrad in tea trees is that the half-life period of tolfenpyrad in a tea tree system is 1.96-3.67 d;
the metabolic rule of tolfenpyrad in tea trees is that no high-toxicity metabolite is generated;
according to the quantity value transfer relationship between tolfenpyrad application dosage and food safety, the maximum allowable tolfenpyrad application dosage of the food safety tolfenpyrad is calculated to be 112.5g a.i./hm2;
The maximum application rate of tolfenpyrad is 112.5g a.i./hm determined according to the above2。
5. The method for calculating the application amount of tolfenpyrad in the tea garden for preventing and treating the tea lesser leafhoppers based on electrostatic spraying according to the claim 1 is characterized in that when the lowest effective dose is calculated, tolfenpyrad is sprayed by an electrostatic sprayer to prevent and treat the tea lesser leafhoppers according to different application doses of tolfenpyrad, the population density of the tea lesser leafhoppers is investigated by adopting a five-point sampling method to obtain the application amount and the pesticide effect of the tolfenpyrad, and the half-life period of the tolfenpyrad in a tea tree system is calculated by pesticide residue data and a kinetic equation; calculating LD of tolfenpyrad on tea lesser leafhopper by tea leaf soaking method test50And LD95。
6. The method for calculating the application amount of tolfenpyrad in tea gardens for controlling tea lesser leafhoppers based on electrostatic spraying according to claim 1, wherein the method for determining the environmental safety threshold value of tolfenpyrad comprises the following steps: according to NY/T2882 'guidelines for evaluating environmental risk of pesticide registration', the allowable concentration value of pesticide residue in the environment is pushed back based on an environmental risk model aiming at tea leafhoppers in a tea tree scene, the pesticide application amount is pushed back according to the standard that the pesticide residue in the environment cannot exceed the risk value of non-target organisms at any moment, and the lowest effective dose of tolfenpyrad is 72g a.i./hm2And a maximum registered dose of 112.5ga.i./hm2Calculating the maximum of tolfenpyradThe large dosage should be less than 112.5g a.i./hm2。
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Application publication date: 20200904 Assignee: SANJIANG DONG AUTONOMOUS COUNTY XIANCHI TEA CO.,LTD. Assignor: Guizhou University Contract record no.: X2023980045628 Denomination of invention: Calculation method of application amount of oxaproxim in tea garden based on electrostatic spray to control tea leafhopper Granted publication date: 20220524 License type: Common License Record date: 20231102 |