CN111603532A

CN111603532A - Composition comprising onion exosomes

Info

Publication number: CN111603532A
Application number: CN202010436972.0A
Authority: CN
Inventors: 程春泉
Original assignee: Xi'an Bohe Medical Technology Co ltd
Current assignee: Xi'an Bohe Medical Technology Co ltd
Priority date: 2020-05-21
Filing date: 2020-05-21
Publication date: 2020-09-01

Abstract

The present invention provides a composition for the prevention of skin scarring, the composition comprising onion exosomes. The composition for preventing skin scars of the present invention further comprises one or more of an active ingredient, a pH adjusting ingredient, a moisturizing ingredient, a stabilizing ingredient, and a preservative ingredient. The composition for preventing the skin scar has good scar defense and relieving effects.

Description

Composition comprising onion exosomes

Technical Field

The invention relates to the field of biomedicine. In particular, the invention relates to compositions comprising onion exosomes.

Background

Plant resources are one of the most important resources for humans, and are important sources of food, medicine, and various living materials. In the aspect of medicine application, different plants generate different secondary metabolites, most of the secondary metabolites have biological activity, and the secondary metabolites are widely applied to various skin care directions such as skin anti-aging (such as asiaticoside), anti-inflammation and soothing (such as bisabolol), whitening and freckle removal (such as anthocyanin) and the like. However, plant efficacy in humans is far more than that of secondary metabolites, and plant exosomes have a variety of similar human regulatory effects, indicating their potential value in skin care.

With the continuous improvement of aesthetic requirements of people, the prevention and treatment requirements on scars after skin injury are higher and higher. Wound healing of the skin is a complex physiological process that includes 4 stages of coagulation, inflammation, proliferation and tissue reconstruction. The method comprises multiple links such as gene expression regulation, secretion regulation of various growth factors and the like, and the abnormality of any link can influence the healing of an incision to cause the formation of scars. In wound repair, TGF-beta is an important fibroblast factor in the fibrosis process, so that antagonistic TGF-beta can effectively prevent and treat excessive fibrosis in the wound healing process, and further effectively prevent scar formation.

Onion extract with antiinflammatory and antibacterial effects. Flavonoids (quercetin and kaempferol) are currently considered as the main components in onion extracts, and inhibit the formation of scars by inhibiting fibroblasts and collagen, softening and flattening the scars and lightening the color. It is also believed that these inhibitory effects may be achieved by quercetin inhibition of transforming growth factor TGF-beta 1 (TGF-beta 1) and TGF-beta 2 and SMAD (Drosophila thermophila transforming growth factor decapentaplegic protein) -related proteins. The study showed that the gel containing onion extract improved the height of the scar and the associated symptoms better, effectively reducing scar formation, compared to the placebo control group. German guidelines for scar treatment and prevention clearly suggest that scar cream containing onion extract can be used as an additional drug for the treatment of scars and for the prevention of post-operative scar formation.

Patent CN103648483A discloses a composition comprising onion extract and liposomes, and relates to a composition comprising a first onion extract (a) and liposomes, wherein at least part of the onion extract is encapsulated in the liposomes, for use in the treatment and/or prevention of scars.

The onion extract contains abundant flavonoid components, can effectively inhibit the formation of scars and has certain cosmetic significance for the generation and treatment of the scars, however, the active substances in the onion extract are water-soluble components and pass through the gaps of the horny layer in the skin absorption process, and the gaps are lipophilic (lipophilic), so that the hydrophilic components are difficult to pass through the horny layer, and the hydrophilic components are still difficult to effectively break through the skin barrier in the application mode of the skin care product and are difficult to be effectively absorbed and utilized by the skin.

Disclosure of Invention

In order to solve the defects of the prior art, the invention provides a composition for preventing skin scars.

The purpose of the invention is realized by the following technical scheme:

in a first aspect, the present invention provides a composition for the prevention of scarring of the skin, the composition comprising onion exosomes.

In a preferred embodiment, the composition further comprises one or more of an active component, a pH adjusting component, a moisturizing component, a stabilizing component, and a preserving component; wherein the content of the first and second substances,

the active component is selected from one or more of plant extract, animal extract, biological fermentation component and chemical synthesis component;

the pH adjusting component is selected from one or more of phosphate buffer, citrate buffer, acetate buffer, Tris buffer and HEPES buffer;

the moisturizing component is selected from one or more of glycerol, propylene glycol, butanediol, 1, 2-pentanediol, polyethylene glycol, polyglycerol, trehalose, sodium hyaluronate, hyaluronic acid, xanthan gum, dextran, rhamnose, sorbitol and carbomer;

the stabilizing component is one or more selected from chelating agent, vitamin E and sodium metabisulfite;

the antiseptic component is one or more selected from glycerol, propylene glycol, butanediol, 1, 2-pentanediol, caprylyl glycol, ethylene glycol, 1, 2-hexanediol, phenoxyethanol, methyl hydroxybenzoate, ethylparaben, propyl hydroxybenzoate, methylisothiazolinone, menthone, caprylhydroxamic acid, sorbic acid and benzoic acid.

In a preferred embodiment, the plant extract is selected from one or more of a purslane extract, a centella asiatica extract, and a butea frondosa extract.

In a preferred embodiment, the animal extract is selected from one or more of an animal umbilical cord extract, an animal placental protein, and an animal thymus extract.

In a preferred embodiment, the biofermentation component is selected from one or more of yeast extract, schizosaccharomyces cerevisiae extract and pichia pastoris fermentation lysate filtrate.

In a preferred embodiment, the chemical synthesis component is selected from one or both of carnosine and ectoin.

In a preferred embodiment, the composition further comprises an organoleptic modifying component selected from one or both of a colour, a flavour and a fragrance.

In a more preferred embodiment, the perfume may use natural or synthetic odoriferous substances or mixtures thereof. Natural odoriferous substances include flowers (lily, lavender, rose, jasmine, orange blossom, ylang), stems and leaves (geranium, patchouli, orange leaf), fruits (fennel, coriander, caraway, juniper berry), fruit shells (bergamot, lemon, mandarin), roots (nutmeg, angelica, celery, cardamom, costus root, iris, thyme), needles and twigs (spruce, fir, pine, mountain pine) and extracts of resins and lipids (bosch resin, elemi, benzoin, myrrh, frankincense, red myrrh). Animal derived perfumes such as civet and castoreum may also be used. Synthetic fragrance compounds such as esters, ethers, aldehydes, ketones, alcohols and hydrocarbon-based products may also be used. Ester-type fragrance compounds such as benzyl acetate, phenoxyethyl isobutyrate, p-tert-butylcyclohexyl acetate, linalyl acetate, phenethyl acetate, linalyl benzoate, benzyl formate, phenylglycine methyl ester, allylcyclohexyl propionate, styryl propionate, and benzyl salicylate; ether-based fragrance compounds such as benzylethyl ether; aldehyde perfume compounds such as linear alkanals having 8 to 18 carbon atoms, citral, citronellal, citronelloxy-acetaldehyde, cyclamen aldehyde, hydroxycitronellal, lilial and bourgeon aldehyde; ketone-based perfume compounds such as ionones, iso-methyl ionone, and methyl cedryl ketone; alcohol perfume compounds include anisole, citronellol, eugenol, isoeugenol, geraniol, linalool, phenethyl alcohol and terpineol; and hydrocarbon fragrance compounds such as terpenes, balsams. It is also possible to use mixtures of different fragrance substances, which together produce an attractive fragrance.

In a second aspect, there is provided a product for the prevention of scarring of the skin, the product comprising a composition according to the invention.

In a third aspect, there is provided use of onion exosomes in the prevention of skin scarring.

In a fourth aspect, there is provided a use of a composition comprising onion exosomes in scar prevention of skin.

In a fifth aspect, there is provided the use of an agent or kit comprising onion exosomes in the prevention of skin scarring.

In a sixth aspect, there is provided use of onion exosomes in the preparation of a product for skin scar prevention.

In a seventh aspect, there is provided the use of a composition comprising onion exosomes in the preparation of a product for skin scar prevention.

In an eighth aspect, there is provided the use of an agent or kit comprising onion exosomes in the preparation of a product for skin scar prevention.

In a ninth aspect, a method of preventing a skin scar, wherein said method comprises the step of using a composition for skin scar prevention according to the present invention.

In a tenth aspect, a method of prevention of skin scarring, wherein the method comprises the step of using a product of skin scarring prevention according to the invention.

In a preferred embodiment, the onion exosomes are prepared by the following method:

s1, adding 250mL of 200-inch sterilized PBS into 250g of 200-inch fresh onion, crushing by using a crusher, centrifuging for 30min at 3000 Xg at 4 ℃ after crushing, removing larger residues, and taking a purple transparent solution in the middle layer;

s2, centrifuging the purple transparent solution obtained in the step S1 at 4 ℃ at 10000 Xg for 60min, and taking supernatant;

s3, centrifuging the supernatant obtained in the step S2 at 4 ℃ and 120000 Xg for 50min, then using 1-2mL sterile PBS to resuspend and precipitate at 4 ℃ and 150000 Xg for 60min, and using 1-2mL sterile PBS to resuspend and precipitate again to obtain the onion exosome.

In a preferred embodiment, the onion exosomes are present in a weight fraction of 0.1 to 30; for example, the onion exosomes are in a weight fraction of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30. The onion exosome can be used for preventing skin scars more effectively when the weight portion of the onion exosome is in the range of 0.1-30.

In a preferred embodiment, the preservative component is present in an amount of 0.1 to 30 parts by weight; for example, the preservative component is present in a weight fraction of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30. The skin scar can be more effectively prevented when the weight part of the preservative component is in the range of 0.1-30.

In a preferred embodiment, the moisture retaining component is present in an amount of 0.1 to 40 parts by weight; for example, the moisturizing component is 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 in parts by weight. The moisture-keeping component can more effectively prevent skin scar when the weight portion is in the range of 0.1-40.

In a preferred embodiment, the weight portion of the active component is 0.1 to 70; for example, the active ingredient is 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 43, 45, 46, 48, 50, 51, 52, 53, 55, 57, 58, 60, 61, 63, 64, 65, 66, 67, 68, 69, or 70 by weight. The active components in the weight portion range of 0.1-70 can more effectively prevent skin scar.

In a preferred embodiment, the weight fraction of the pH adjusting component is 0.01 to 5; for example, the pH adjusting component is 0.01, 0.02, 0.03, 0.05, 0.06, 0.07, 0.08, 0.1, 0.2, 0.3, 0.5, 0.7, 0.8, 1, 1.3, 1.5, 1.7, 2, 2.2, 2.5, 2.7, 2.8, 3, 3.2, 3.5, 3.7, 3.8, 4, 4.1, 4.3, 4.5, 4.7, 4.9, or 5 in parts by weight. The pH adjusting component can more effectively prevent skin scar when the weight portion is in the range of 0.01-5.

In a preferred embodiment, the stabilizing component is present in an amount of 0.1 to 10 parts by weight; for example, the stabilizing component is present in a weight fraction of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1,2, 3, 4, 5, 6, 7, 8, 9, or 10. The stable component can more effectively prevent skin scar when the weight portion is in the range of 0.1-10.

In a preferred embodiment, the sensory modifying component is present in an amount of 0.1 to 50 parts by weight; for example, the sensory modifying component is 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or 50 parts by weight. The sensory modification component can more effectively prevent skin scar when the weight portion is in the range of 0.1-50.

In an eleventh aspect, there is provided a process for the preparation of a composition for the prevention of skin scarring according to the present invention, comprising the steps of:

s1, mixing, heating and stirring an active component, a pH adjusting component, a moisturizing component, a stabilizing component, an organoleptic restoration component and water to dissolve the active component, the pH adjusting component, the moisturizing component, the stabilizing component and the organoleptic restoration component;

s2, adding the onion exosomes and the anticorrosive components when the temperature reaches 30 ℃, uniformly stirring, and discharging.

In a preferred embodiment, the use is with a kit as described above.

In the present invention, the composition or kit of the present invention may further comprise other auxiliary ingredients commonly used in the art.

In the present invention, features, embodiments, preferred embodiments described for one aspect are equally applicable to other aspects of the invention.

In preferred embodiments, the compositions, reagents, kits are described as "comprising" or "including". It will be understood by those skilled in the art that the compositions, reagents, kits may consist essentially of, or consist of, the respective components, reagents.

The description of the invention should be understood to include various embodiments or combinations of elements of the invention and combinations of alternatives thereof, except where expressly incompatible with the objects of the invention.

Compared with the prior art, the invention has the beneficial effects that:

the onion source components applied to scar and scar care at present are plant extract components, and the types and the contents of active substances of onion extracts of different manufacturers are greatly different due to the difference of extraction methods and processes. Meanwhile, the activity of the onion extract is lost due to the instability of the active ingredients of the onion extract. In addition, due to the barrier function of the skin, it is difficult for these actives to reach the deeper layers of the skin where living cells function.

The active component of the onion exosome is different from the component of the onion extract, the outside is a special phospholipid bilayer structure, the inside is wrapped with active onion secondary metabolite and miRNA component with regulation and control effects, the phospholipid bilayer structure can protect the active component on one hand, on the other hand, the gap can pass through the skin horny layer, the gap is lipophilic (lipophilic), due to the barrier function of the skin, the hydrophilic component hardly passes through the horny layer, the secondary metabolite and miRNA component contained in the onion exosome can reduce the generation and growth of scars, and the phospholipid bilayer structure of the onion exosome can enable the active component contained in the onion exosome to reach the acting part more easily, so that the biological activity of the onion exosome is exerted, and the better scar defense and relief effects are achieved.

The onion exosome is applied to a composition for preventing skin scars or a product for preventing skin scars and is used for nursing the scar skin, the onion exosome in the composition or the product has a stable structure and can effectively protect the contained active ingredients, and meanwhile, the external structure of the onion exosome is a phospholipid bilayer structure which is the same as that of skin cells, so that the onion exosome is more easily absorbed by the cells and plays a role in nursing the scar.

In addition, the invention provides a brand new onion active structure, namely onion exosome, and applies the onion active structure to skin care products in different dosage forms and forms. The onion exosome has consistent external structure and contains various onion active ingredients, so that the extraction process is stable, the product property is stable, and no layering or precipitation exists.

Detailed Description

The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.

The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

Example 1

This example provides a composition for the prevention of scarring of the skin comprising onion exosomes.

In some embodiments, the composition further comprises one or more of an active component, a pH adjusting component, a moisturizing component, a stabilizing component, and a preserving component.

In some embodiments, the pH-adjusting component is selected from one or more of phosphate buffer, citrate buffer, acetate buffer, Tris buffer, and HEPES buffer.

In some embodiments, the moisturizing component is selected from one or more of glycerin, propylene glycol, butylene glycol, 1, 2-pentanediol, polyethylene glycol, polyglycerol, trehalose, sodium hyaluronate, hyaluronic acid, xanthan gum, dextran, rhamnose, sorbitol, and carbomer.

In some embodiments, the stabilizing component is selected from one or more of a chelating agent, vitamin E, and sodium metabisulfite.

In some embodiments, the preservative component is selected from one or more of glycerol, propylene glycol, butylene glycol, 1, 2-pentanediol, caprylyl glycol, ethylene glycol, 1, 2-hexanediol, phenoxyethanol, methyl paraben, ethyl paraben, propyl paraben, methylisothiazolinone, menthone, caprylhydroxamic acid, sorbic acid, and benzoic acid.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

In some embodiments, the active component is selected from one or more of a plant extract, an animal extract, a biofermentation component, and a chemical synthesis component.

In particular embodiments, the plant extract is selected from one or more of a purslane extract, a centella asiatica extract, and a butea frondosa extract.

In particular embodiments, the animal extract is selected from one or more of an animal umbilical cord extract, an animal placental protein, and an animal thymus extract.

In particular embodiments, the biofermentation component is selected from one or more of yeast extract, schizosaccharomyces cerevisiae extract, and pichia pastoris fermentation lysate filtrate.

In particular embodiments, the chemically synthesized component is selected from one or both of carnosine and ectoin.

Example 2

This example provides a product for the prevention of scarring of the skin comprising the composition of example 1.

Example 3

This example provides the use of onion exosomes in the prevention of skin scarring.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 4

This example provides the use of a composition comprising onion exosomes in the prevention of skin scarring.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 5

This example provides the use of an agent comprising onion exosomes in the prevention of skin scarring.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 6

This example provides the use of a kit comprising onion exosomes in the prevention of skin scarring.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 7

This example provides the use of onion exosomes in the preparation of a product for skin scar prevention.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 8

This example provides the use of a composition comprising onion exosomes in the preparation of a product for skin scar prevention.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 9

This example provides the use of an agent comprising onion exosomes in the preparation of a product for skin scar prevention.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 10

This example provides the use of a kit comprising onion exosomes in the preparation of a product for skin scar prevention.

In some embodiments, the onion exosomes are in a weight fraction of 0.1-30.

Example 11

The onion exosomes were studied as shown in table 1 below.

TABLE 1 dose study of onion exosomes

Example 12

This example provides a composition for the prevention of skin scarring, wherein the composition comprises, in parts by weight:

0.1 part of onion exosome, 70 parts of centella asiatica extract, 0.5 part of citric acid buffer solution, 15 parts of glycerol, 1 part of sodium metabisulfite, 1 part of phenoxyethanol, 0.1 part of essence and a proper amount of water.

Example 13

30 parts of onion exosomes, 0.1 part of centella asiatica extract, 0.5 part of citric acid buffer solution, 15 parts of glycerol, 1 part of sodium metabisulfite, 1 part of phenoxyethanol, 0.1 part of essence and a proper amount of water.

Comparative example 1

30 parts of onion extract, 0.1 part of centella extract, 0.5 part of citric acid buffer solution, 15 parts of glycerol, 1 part of sodium metabisulfite, 1 part of phenoxyethanol and 0.1 part of essence, and the balance of water.

Comparative example 2

To further illustrate the beneficial effects of the present invention, there is provided a composition comprising onion extract and liposomes, which is prepared by the method in CN 103648483A.

Experiment 1: improvement of scars or scars

And (3) testing a sample: examples 11 to 13, comparative examples 1 to 2

The experimental method comprises the following steps: 150 volunteers with scars or scars are selected, the scars or the scars of the volunteers are formed just within half a year, and the volunteers are divided into 10 persons in each group according to the condition of the scars or the scars. The volunteers applied the test samples on the scars or scars 2 times a day for a period of 6 weeks, and finally evaluated from 3 aspects of the color (red), width and height of the scars or scars.

Wherein, the color of the scar or scar is measured by a DermaLab skin tester, the width is measured by a vernier caliper, and the height of the scar at the edge fixing position is measured by the vernier caliper. 10 volunteers of each experimental group were tested and calculated before and after use (before use-after use), and the results were averaged as shown in Table 1 below.

TABLE 1 improvement of scars or scars

As can be seen from table 1, the composition for preventing skin scar provided by the present invention can improve scar condition well. The scar condition can be remarkably improved by screening the types of the components in the composition for preventing the skin scar or adjusting the weight parts of the components in the composition for preventing the skin scar. While the improvement in scarring is not apparent by using a composition that replaces a component of, or adjusts a part by weight of, a composition for the prevention of scarring of the skin of the present invention, the present invention illustrates that the improvement in scarring is not ideal for a composition for the prevention of scarring of the skin that is obtained by replacing a component of, or adjusting a part by weight of, a composition for the prevention of scarring of the skin of the present invention.

Experiment 2: stability verification

And (3) testing a sample: examples 11 to 13, comparative examples 1 to 2

The experimental method comprises the following steps:

the tested samples are tested for 15 days, 30 days and 40 days under four test conditions of-18 ℃, room temperature, 45 ℃ and-18-45 ℃ alternately, and the tested samples are observed to have obvious change, pass or fail, and the test results are shown in the following table 2.

Table 2 stability test results

From the above results, it can be seen that:

after the composition for preventing the skin scar is tested for 15 days, 30 days and 40 days under four test conditions of-18 ℃, room temperature, 45 ℃ and-18-45 ℃ alternately, the test results are all qualified, namely the composition for preventing the skin scar has good cold resistance and heat resistance and can meet the use requirements under the condition of extreme temperature change. The composition for preventing the skin scar, which does not use the formula system, has poor cold resistance and heat resistance under the condition of long-time storage, and cannot meet the use requirement under the condition of extreme temperature change.

It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.

Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Claims

1. A composition for the prevention of skin scarring, the composition comprising onion exosomes.

2. The composition of claim 1, further comprising one or more of an active component, a pH adjusting component, a moisturizing component, a stabilizing component, and a preserving component; wherein the content of the first and second substances,

3. A product for the prevention of skin scarring comprising the composition of claims 1-2.

4. Use of onion exosomes for the prevention of skin scarring.

5. Use of a composition comprising onion exosomes for the prevention of skin scarring.

6. Use of an agent or kit comprising onion exosomes for skin scar prevention.

7. Use of onion exosomes in the preparation of a product for the prevention of skin scarring.

8. Use of a composition comprising onion exosomes for the preparation of a product for the prevention of skin scarring.

9. Use of a reagent or kit comprising onion exosomes for the preparation of a product for skin scar prevention.

10. The composition according to claims 1-2 or the use according to claims 4-9, wherein the onion exosomes are present in a weight fraction of 0.1-30.