CN111592963A - Probiotic fermentation barrel used in culture site immediately and use method - Google Patents
Probiotic fermentation barrel used in culture site immediately and use method Download PDFInfo
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- CN111592963A CN111592963A CN202010338315.2A CN202010338315A CN111592963A CN 111592963 A CN111592963 A CN 111592963A CN 202010338315 A CN202010338315 A CN 202010338315A CN 111592963 A CN111592963 A CN 111592963A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention discloses a probiotic fermentation barrel for immediate use in a culture site, which comprises a barrel body, a barrel cover, a rotating shaft, an inflation pipe, an aeration disc, a motor and other main parts, and also comprises the following accessories attached to the main parts: the fermentation vat comprises a buckle, a silica gel ring, a semi-ring, an inflation valve, an inflation tube, a liquid outlet valve, an air outlet tube, a breathable film, a shaft ring and a stirring paddle sheet, wherein the total volume of the fermentation vat is 50-200L, and the effective liquid filling amount is 40-160L. The invention also provides a method for using the probiotic fermentation barrel. The invention can be widely used in aquatic farms and terrestrial animal farms, has the advantages of simple operation, low equipment cost and convenient placement and movement, can culture a large amount of live bacteria in time for use, truly exerts the effect of probiotics and saves the purchase cost of a large amount of probiotics.
Description
Technical Field
The invention relates to a fermentation vat, in particular to liquid strain fermentation equipment for beneficial bacteria in aquaculture and terrestrial economic animal culture.
Background
In recent years, with the rapid development of large-scale and high-density aquaculture modes and the development of industrial aquaculture of terrestrial economic animals, a plurality of problems occur in the aquaculture process, such as deterioration of aquaculture environment and frequent occurrence of aquaculture diseases, which bring huge economic losses to the aquaculture industry, and on the other hand, the use of antibiotic drugs is increasingly strictly controlled in animal aquaculture. Under the trend, the use of probiotics in cultivation is more and more extensive, and is generally confirmed and accepted by farmers and cultivation enterprises.
Microbial preparation products currently sold in the field of aquaculture are of various types, and the main probiotic types include: the probiotics play an important role in the aspects of regulating and controlling water quality, promoting animal growth, inhibiting diseases, treating animal excrement and the like, and have important significance for reducing cultivation waste and wastewater discharge and protecting the environment. However, we found that many probiotics do not exert the effect of the product in the culture terminal in the actual use process of the probiotic product, and the problems are found in several aspects through investigation and experiments: (1) most microbial preparation products are not transported in a cold chain and refrigerated at low temperature, and most products are randomly placed in a high-temperature environment in a terminal sales shop, so that the number of live bacteria in the microbial preparation products is remarkably reduced, even no needed live bacteria exist; (2) the period from production to final arrival at a farm to use is too long, so that the number of live bacteria is obviously reduced; (3) the quality of the product is uneven, most manufacturers dilute a large amount of fermentation stock seeds in a large proportion in order to save cost, so that the actual product does not have the declared number of viable bacteria; (4) the probiotic product is directly used or simply soaked and fermented, so that the actually used beneficial live bacteria are few, and the effect cannot be achieved at all.
Based on the above problems, it is necessary to activate the strain and expand the culture sufficiently before the strain is put into use, so as to actually exert the effect of the probiotics. However, no equipment capable of instantly fermenting on site is equipped in the current farm, no fermentation equipment which can be used for the farm is produced in the market, the existing fermentation equipment only aims at the scientific research (small amount) in a laboratory or large-scale probiotic manufacturers (huge amount), the equipment cost is high, the specialization is strong, and the equipment is completely not suitable for being used in the farm.
Disclosure of Invention
In order to solve the problems, the invention provides a probiotic fermentation barrel which can be used in a culture site immediately.
A probiotic fermentation barrel used immediately in a culture site comprises a barrel body, a barrel cover, a rotating shaft, an inflation pipe, an aeration disc and a motor; the outer side of the barrel body is provided with a buckle for buckling the barrel cover, the inner side of the barrel body is provided with a row of semi-rings for the inflation tube to pass through to fix the position of the inflation tube from top to bottom, the upper side of the barrel body is provided with an opening, an inflation valve is arranged on the opening, the lower side of the barrel body is provided with an opening, and a liquid outlet valve is arranged on the; the aeration disc is arranged in the center of the bottom of the barrel body, the interface of the aeration disc is connected with one end of an inflation pipe, the other end of the inflation pipe is connected with the inner side end of an inflation valve, and the outer side end of the inflation valve is connected with a pure oxygen bottle through a gas pipe; the center of the barrel cover is provided with a hole, the upper part and the lower part of the hole are respectively provided with a shaft collar for fixing the rotating shaft, the barrel cover is also provided with an air outlet pipe for ventilation, and the pipe orifice is provided with a breathable film; one end of the rotating shaft extending into the barrel body is provided with a stirring blade, and the other end of the rotating shaft is connected with a motor.
Preferably, the fermentation barrel is made of stainless steel.
Preferably, the inflation valve, the liquid outlet valve and the air outlet pipe are made of stainless steel materials.
Preferably, the inflation tube is made of silica gel.
Preferably, the total volume of the fermentation barrel is 50-200L, and the effective liquid filling amount is 40-160L.
The invention also provides a method for using the probiotic fermentation barrel, which comprises the following steps:
(1) adding tap water into the barrel body, adding chlorine dioxide according to the dosage of the specification, starting a motor after disinfection, and rotating to aerate for more than 6 hours;
(2) adding the sterilized concentrated probiotic culture medium into the barrel body according to the dosage, and inoculating a proper amount of probiotic live bacteria;
(3) covering the barrel cover, buckling the barrel cover by a buckle on the barrel body, covering the air outlet pipe orifice on the barrel cover by a breathable film, and tightly rolling by a rope to keep sealing;
(4) connecting the outer side end of the inflation valve with the gas pipe of the oxygen cylinder, and opening the inflation valve;
(5) turning on a motor to drive a rotating shaft to operate, opening a liquid outlet valve in the culture fermentation process, and sampling to observe the culture state of the probiotics;
(6) when the air temperature is lower, the barrel body is properly heated, the culture temperature is increased, and the fermentation culture process is accelerated.
Compared with the prior art, the invention has the beneficial effects that:
1) the fermentation vat is cheap, and the plant all has the ability purchase to easy operation, the size is suitable, places and removes conveniently, provides solid foundation for its large-scale popularization.
2) By using the probiotic fermentation barrel and the matched invention method, a large amount of live bacteria can be obtained in a culture site in time, and the effectiveness of the probiotics can be really exerted.
3) And a small amount of strains are adopted for fermentation and expansion, so that the use cost of the probiotics is greatly saved.
Drawings
Fig. 1 is a schematic structural diagram of a probiotic fermenter according to the present invention;
description of reference numerals: 1: a barrel body; 2: a barrel cover; 3: a rotating shaft; 4: an inflation tube; 5: an aeration disc; 6: a motor; 1-1: buckling; 1-2: a half ring; 1-3: an inflation valve; 1-4: a liquid outlet valve; 1-5: max scale lines; 2-1: a silica gel ring; 2-2: an air outlet pipe; 2-3: a gas permeable membrane; 2-4: a collar; 3-1: and (7) pulping.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example 1
As shown in figure 1, the probiotic fermentation barrel capable of being used immediately in a culture site has the total capacity of 50L and the maximum effective capacity of 40L, and comprises a barrel body 1, a barrel cover 2, a rotating shaft 3, an inflation pipe 4, an aeration disc 5, a motor 6 and other main parts.
The barrel body 1 is made of stainless steel materials, three buckles 1-1 are welded on the outer side of the barrel body 1 and used for buckling the barrel cover 2 and keeping sealing, a row of semi-rings 1-2 are welded on the inner side of the barrel body 1 from top to bottom, an inflation tube 4 penetrates through the semi-rings 1-2 and is fixed in position, a hole is formed in the upper side of the barrel body 1, a stainless steel inflation valve 1-3 is welded on the inner side of the barrel body 1, a stainless steel liquid outlet valve 1-4 is welded on the lower side of the barrel body 1, and a Max scale mark 1-5 is arranged on the upper. The aeration disc 5 is arranged in the center of the bottom of the barrel body 1, the interface of the aeration disc 5 is connected with one end of an inflation tube 4, the inflation tube 4 is made of silica gel, the other end of the inflation tube 4 is connected with the inner side end of an inflation valve 1-3, and the outer side end of the inflation valve 1-3 is connected with a pure oxygen bottle through a gas pipe. The barrel cover 2 is made of stainless steel, a hole is formed in the center of the barrel cover 2, a shaft collar 2-1 is welded to the upper portion and the lower portion of the hole respectively and used for fixing the rotating shaft 3, a stainless steel air outlet pipe 2-2 is welded to the other hole of the barrel cover 2 and used for ventilation, and the air outlet pipe 2-2 is covered with a breathable film 2-3 and is tightly rolled by a rope. A sealing ring 2-4 is fixed at the bottom of the barrel cover 2 and is made of silica gel and used for sealing the barrel body 1 and the barrel cover 2. The lower end of the rotating shaft 3 is welded with a stirring paddle 3-1, and the upper end is welded or clamped with a motor 6.
Example 2
As shown in figure 1, the probiotic fermentation barrel capable of being used immediately in a culture site has the total capacity of 100L and the maximum effective capacity of 80L, and comprises a barrel body 1, a barrel cover 2, a rotating shaft 3, an inflation pipe 4, an aeration disc 5, a motor 6 and other main parts.
The barrel body 1 is made of stainless steel materials, three buckles 1-1 are welded on the outer side of the barrel body 1 and used for buckling the barrel cover 2 and keeping sealing, a row of semi-rings 1-2 are welded on the inner side of the barrel body 1 from top to bottom, an inflation tube 4 penetrates through the semi-rings 1-2 and is fixed in position, a hole is formed in the upper side of the barrel body 1, a stainless steel inflation valve 1-3 is welded on the inner side of the barrel body 1, a stainless steel liquid outlet valve 1-4 is welded on the lower side of the barrel body 1, and a Max scale mark 1-5 is arranged on the upper. The aeration disc 5 is arranged in the center of the bottom of the barrel body 1, the interface of the aeration disc 5 is connected with one end of an inflation tube 4, the inflation tube 4 is made of silica gel, the other end of the inflation tube 4 is connected with the inner side end of an inflation valve 1-3, and the outer side end of the inflation valve 1-3 is connected with a pure oxygen bottle through a gas pipe. The barrel cover 2 is made of stainless steel, a hole is formed in the center of the barrel cover 2, a shaft collar 2-1 is welded to the upper portion and the lower portion of the hole respectively and used for fixing the rotating shaft 3, a stainless steel air outlet pipe 2-2 is welded to the other hole of the barrel cover 2 and used for ventilation, and the air outlet pipe 2-2 is covered with a breathable film 2-3 and is tightly rolled by a rope. A sealing ring 2-4 is fixed at the bottom of the barrel cover 2 and is made of silica gel and used for sealing the barrel body 1 and the barrel cover 2. The lower end of the rotating shaft 3 is welded with a stirring paddle 3-1, and the upper end is welded or clamped with a motor 6.
Example 3
As shown in figure 1, the probiotic fermentation barrel capable of being used immediately in a culture site has the total volume of 200L and the maximum effective volume of 160L, and comprises a barrel body 1, a barrel cover 2, a rotating shaft 3, an inflation pipe 4, an aeration disc 5, a motor 6 and other main parts.
The barrel body 1 is made of stainless steel materials, three buckles 1-1 are welded on the outer side of the barrel body 1 and used for buckling the barrel cover 2 and keeping sealing, a row of semi-rings 1-2 are welded on the inner side of the barrel body 1 from top to bottom, an inflation tube 4 penetrates through the semi-rings 1-2 and is fixed in position, a hole is formed in the upper side of the barrel body 1, a stainless steel inflation valve 1-3 is welded on the inner side of the barrel body 1, a stainless steel liquid outlet valve 1-4 is welded on the lower side of the barrel body 1, and a Max scale mark 1-5 is arranged on the upper. The aeration disc 5 is arranged in the center of the bottom of the barrel body 1, the interface of the aeration disc 5 is connected with one end of an inflation tube 4, the inflation tube 4 is made of silica gel, the other end of the inflation tube 4 is connected with the inner side end of an inflation valve 1-3, and the outer side end of the inflation valve 1-3 is connected with a pure oxygen bottle through a gas pipe. The barrel cover 2 is made of stainless steel, a hole is formed in the center of the barrel cover 2, a shaft collar 2-1 is welded to the upper portion and the lower portion of the hole respectively and used for fixing the rotating shaft 3, a stainless steel air outlet pipe 2-2 is welded to the other hole of the barrel cover 2 and used for ventilation, and the air outlet pipe 2-2 is covered with a breathable film 2-3 and is tightly rolled by a rope. A sealing ring 2-4 is fixed at the bottom of the barrel cover 2 and is made of silica gel and used for sealing the barrel body 1 and the barrel cover 2. The lower end of the rotating shaft 3 is welded with a stirring paddle 3-1, and the upper end is welded or clamped with a motor 6.
Example 4
A method for using the probiotic fermenter as shown in fig. 1, which can be used immediately in a cultivation site, comprises the following steps:
(1) adding 80L of tap water (which can not exceed Max scale marks) into the barrel body 1, adding chlorine dioxide according to the use amount of the specification, starting a motor after disinfection, and rotating to aerate for more than 6 hours;
(2) adding 4L (sterilized) of 20 multiplied concentrated probiotic culture medium into the barrel body 1, and inoculating 80ml of probiotic live bacteria;
(3) carefully covering the barrel cover 2, buckling the barrel cover 2 by a buckle on the barrel body 1, covering a port 2-2 of an air outlet pipe on the barrel cover 2 by a breathable film 2-3, and tightly rolling by a rope;
(4) the outer side end of the inflation valve 1-3 is connected with the gas pipe of the oxygen cylinder, and the inflation valve 1-3 is opened.
(5) Turning on the motor 6 to drive the rotating shaft 3 to operate, and opening the liquid outlet valves 1-4 in the culture fermentation process for sampling to observe the culture state of the probiotics;
(6) when the air temperature is lower, the barrel body 1 can be properly heated to improve the culture temperature and accelerate the fermentation culture process.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention accordingly, and not to limit the protection scope of the present invention accordingly. All equivalent changes or modifications made in accordance with the spirit of the present disclosure are intended to be covered by the scope of the present disclosure.
Claims (6)
1. The utility model provides a probiotics fermenter of breed on-the-spot instant use which characterized in that: comprises a barrel body, a barrel cover, a rotating shaft, an inflation pipe, an aeration disc and a motor; the outer side of the barrel body is provided with a buckle for buckling the barrel cover, the inner side of the barrel body is provided with a row of semi-rings for the inflation tube to pass through to fix the position of the inflation tube from top to bottom, the upper side of the barrel body is provided with an opening, an inflation valve is arranged on the opening, the lower side of the barrel body is provided with an opening, and a liquid outlet valve is arranged on the; the aeration disc is arranged in the center of the bottom of the barrel body, the interface of the aeration disc is connected with one end of an inflation pipe, the other end of the inflation pipe is connected with the inner side end of an inflation valve, and the outer side end of the inflation valve is connected with a pure oxygen bottle through a gas pipe; the center of the barrel cover is provided with a hole, the upper part and the lower part of the hole are respectively provided with a shaft collar for fixing the rotating shaft, the barrel cover is also provided with an air outlet pipe for ventilation, and the pipe orifice is provided with a breathable film; one end of the rotating shaft extending into the barrel body is provided with a stirring blade, and the other end of the rotating shaft is connected with a motor.
2. The probiotic fermenter for use in a cultivation site, according to claim 1, wherein: the fermentation barrel is made of stainless steel.
3. The probiotic fermenter for use in a cultivation site, according to claim 2, wherein: the charging valve, the liquid outlet valve and the air outlet pipe are made of stainless steel materials.
4. The probiotic fermenter for use in a cultivation site, according to claim 1, wherein: the gas tube be the silica gel material.
5. The probiotic fermenter for use in a cultivation site, according to claim 1, wherein: the total volume of the fermentation barrel is 50-200L, and the effective liquid loading amount is 40-160L.
6. A method of using a probiotic fermenter according to any of claims 1 to 5, wherein: the method comprises the following steps:
(1) adding tap water into the barrel body, adding chlorine dioxide according to the dosage of the specification, starting a motor after disinfection, and rotating to aerate for more than 6 hours;
(2) adding the sterilized concentrated probiotic culture medium into the barrel body according to the dosage, and inoculating a proper amount of probiotic live bacteria;
(3) covering the barrel cover, buckling the barrel cover by a buckle on the barrel body, covering the air outlet pipe orifice on the barrel cover by a breathable film, and tightly rolling by a rope to keep sealing;
(4) connecting the outer side end of the inflation valve with the gas pipe of the oxygen cylinder, and opening the inflation valve;
(5) turning on a motor to drive a rotating shaft to operate, opening a liquid outlet valve in the culture fermentation process, and sampling to observe the culture state of the probiotics;
(6) when the air temperature is lower, the barrel body is properly heated, the culture temperature is increased, and the fermentation culture process is accelerated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010338315.2A CN111592963A (en) | 2020-04-26 | 2020-04-26 | Probiotic fermentation barrel used in culture site immediately and use method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010338315.2A CN111592963A (en) | 2020-04-26 | 2020-04-26 | Probiotic fermentation barrel used in culture site immediately and use method |
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| CN111592963A true CN111592963A (en) | 2020-08-28 |
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| CN202010338315.2A Pending CN111592963A (en) | 2020-04-26 | 2020-04-26 | Probiotic fermentation barrel used in culture site immediately and use method |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB899478A (en) * | 1958-03-01 | 1962-06-20 | Gerd Becker | Improvements in or relating to apparatus for obtaining yeast |
| CN202311141U (en) * | 2011-11-18 | 2012-07-11 | 山东宝来利来生物工程股份有限公司 | Lactic acid bacteria FLF (fermented liquid feed) autofermentation system specially for meadow |
| CN203034001U (en) * | 2013-01-18 | 2013-07-03 | 王玉焕 | Ecological breeding probiotics fermentation tank |
| CN103583850A (en) * | 2012-08-17 | 2014-02-19 | 广州市纽溢乐商贸有限公司 | Preparation method of composite microecologics, and fermenting equipment of preparation method |
| CN205443294U (en) * | 2015-12-30 | 2016-08-10 | 山东植丰农化集团有限公司 | Device for producing microbial preparation |
| CN206580820U (en) * | 2017-03-28 | 2017-10-24 | 陕西理工大学 | A kind of novel microbial fermentation tank |
| CN108949513A (en) * | 2018-07-25 | 2018-12-07 | 杭州宸祥环境工程有限公司 | A kind of microbial ferment device |
| CN208649293U (en) * | 2018-07-27 | 2019-03-26 | 沈阳工学院 | A kind of fermentation tank for liquid culture of edible fungi |
| CN208667694U (en) * | 2018-08-15 | 2019-03-29 | 沈阳工学院 | A kind of microbial ferment device |
-
2020
- 2020-04-26 CN CN202010338315.2A patent/CN111592963A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB899478A (en) * | 1958-03-01 | 1962-06-20 | Gerd Becker | Improvements in or relating to apparatus for obtaining yeast |
| CN202311141U (en) * | 2011-11-18 | 2012-07-11 | 山东宝来利来生物工程股份有限公司 | Lactic acid bacteria FLF (fermented liquid feed) autofermentation system specially for meadow |
| CN103583850A (en) * | 2012-08-17 | 2014-02-19 | 广州市纽溢乐商贸有限公司 | Preparation method of composite microecologics, and fermenting equipment of preparation method |
| CN203034001U (en) * | 2013-01-18 | 2013-07-03 | 王玉焕 | Ecological breeding probiotics fermentation tank |
| CN205443294U (en) * | 2015-12-30 | 2016-08-10 | 山东植丰农化集团有限公司 | Device for producing microbial preparation |
| CN206580820U (en) * | 2017-03-28 | 2017-10-24 | 陕西理工大学 | A kind of novel microbial fermentation tank |
| CN108949513A (en) * | 2018-07-25 | 2018-12-07 | 杭州宸祥环境工程有限公司 | A kind of microbial ferment device |
| CN208649293U (en) * | 2018-07-27 | 2019-03-26 | 沈阳工学院 | A kind of fermentation tank for liquid culture of edible fungi |
| CN208667694U (en) * | 2018-08-15 | 2019-03-29 | 沈阳工学院 | A kind of microbial ferment device |
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Application publication date: 20200828 |
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