CN111574291B

CN111574291B - Bacillus polymyxa water-soluble fertilizer and preparation method and application thereof

Info

Publication number: CN111574291B
Application number: CN202010275302.5A
Authority: CN
Inventors: 吕昆明; 廖华; 廖博; 杨瑞青; 陆明光; 陆可敬; 吕贵升
Original assignee: Guangxi Letu Biological Technology Co ltd
Current assignee: Guangxi Letu Biological Technology Co ltd
Priority date: 2020-04-09
Filing date: 2020-04-09
Publication date: 2022-01-14
Anticipated expiration: 2040-04-09
Also published as: CN111574291A

Abstract

The invention discloses a water-soluble fertilizer for bacillus polymyxa and a preparation method and application thereof, wherein the water-soluble fertilizer comprises bacillus polymyxa; the bacillus polymyxa is preserved in China center for type culture Collection with the preservation number of CCTCC No: the sequence of M2019116, 16srRNA is shown in SEQ ID No. 1; the preparation process comprises the following steps: the water-soluble fertilizer can effectively prevent and treat citrus canker, reduce the morbidity of the citrus canker and effectively promote the growth of plants in the processes of weighing, mixing and packaging.

Description

Bacillus polymyxa water-soluble fertilizer and preparation method and application thereof

Technical Field

The invention relates to the technical field of biological fertilizers, and particularly relates to a bacillus polymyxa water-soluble fertilizer and a preparation method and application thereof.

Background

Citrus canker is a disease caused by pseudomonas flavipes, and is frequently found in citrus; the disease is harmful to citrus leaves, branches and tips and fruits, and leaves fall and withered tips are caused when nursery stocks and young trees are seriously damaged, so that the tree vigor is influenced; the fruit victims fall off fruits and the light victims have scabs and are not resistant to storage and rot, so that the commodity value of the fruits is greatly reduced, the pest control cost is increased for fruit growers, and the economic benefit is damaged.

At the present stage, the method of spraying pesticides on citrus leaves is usually used for preventing and treating the pseudomonas flavipes, but on one hand, the pesticides are easy to cause pathogenic bacteria to generate drug resistance, so that the prevention and treatment difficulty is reduced; on the other hand, the environment is polluted.

The bacillus polymyxa is a kind of rhizosphere growth-promoting bacteria with wide hosts, and has control effects on plant diseases caused by fungi, bacteria, nematodes and the like; it can produce a plurality of antibiotics, polymyxin, various hydrolytic enzymes and other antagonistic substances, and the antagonistic substances are one of the important mechanisms of the biological control function of the bacillus polymyxa. Meanwhile, the bacillus polymyxa can induce plants to produce salicylic acid, so that Induced Systemic Resistance (ISR) is triggered; the bacillus polymyxa can also synthesize auxin, cytokinin and exopolysaccharide, and has activities of dissolving phosphorus and fixing nitrogen. If the bacillus polymyxa can be prepared into a water-soluble fertilizer for preventing and treating the citrus canker, the bacillus polymyxa can not only effectively prevent pathogenic bacteria, but also prevent and treat environmental pollution.

Therefore, the technical personnel in the field need to solve the problem of how to provide a bacillus polymyxa water-soluble fertilizer capable of effectively preventing and treating citrus canker as well as a preparation method and application thereof.

Disclosure of Invention

In view of the above, the invention provides a bacillus polymyxa water-soluble fertilizer and a preparation method and application thereof, and the water-soluble fertilizer can effectively prevent and treat citrus canker, reduce the morbidity of the citrus canker and simultaneously effectively promote the growth of plants.

In order to achieve the purpose, the invention adopts the following technical scheme:

a water-soluble fertilizer of bacillus polymyxa comprises: bacillus polymyxa; the bacillus polymyxa is preserved in China center for type culture Collection, the preservation address is China, Wuhan university, the preservation number is CCTCC No: m2019116, 16srRNA sequence is shown in SEQ ID No. 1.

The technical effect achieved by the technical scheme is as follows: compared with other bacillus, the bacillus polymyxa LTPP03 has stronger disease resistance; the fertilizer can enhance the nitrogen fixation capacity of soil and effectively promote plant growth, so that the fertilizer can be prepared into water-soluble fertilizer and sprayed on plant leaf surfaces to effectively prevent and treat citrus canker.

As a preferred embodiment of the present invention, the method further comprises: 350g of amino acid raw powder, 0-100g of ammonium sulfate, 0-50g of nitrogen-potassium compound fertilizer, 100g of potassium sulfate, 0-100g of diammonium hydrogen phosphate, 109g of magnesium sulfate monohydrate, 50g of boric acid, 50g of zinc sulfate monohydrate, 5g of manganese glycinate, 10g of iron glycinate, 1g of ammonium heptamolybdate, 5g of copper glycinate, 60g of surfactant and 0-20g of anti-caking powder.

The technical effect achieved by the technical scheme is as follows: macroelements, secondary elements and trace elements are combined together to provide necessary nutritional support for the growth and development of plants; the amino acid contains 18 kinds of free amino acid, the total amount of all amino acid is about 73.1%, the amino acid has unique promotion function on plant growth, especially photosynthesis, especially glycine, can increase the chlorophyll content of plants, improve the activity of enzyme, promote the penetration of carbon dioxide, enable the photosynthesis to be more vigorous, and have important functions on improving the crop quality and increasing the content of Vc and sugar; the anti-caking can prevent the components from being bonded into blocks without influencing the fertilizer efficiency; because the anti-caking agent is generally composed of organic substances and is incompatible with inorganic substances such as major elements, medium elements, trace elements and the like, the surfactant has fixed hydrophilic and lipophilic groups, can be directionally arranged on the surface of a solution, can obviously reduce the surface tension, and further enables the anti-caking agent to be compatible with the inorganic substances.

As a preferable technical scheme of the invention, the water-soluble fertilizer contains 40g of LTPP03 bacterial powder, and the content of LTPP03 bacterial cells in each gram of bacterial powder is 50 hundred million/g.

As a preferred embodiment of the present invention, the surfactant includes: the sodium methylene dinaphthalene sulfonate and the sodium lignin sulfonate are mixed according to the mass ratio of 1 (2-11).

As a preferred technical solution of the present invention, the anti-caking agent comprises: any two or three of polyacrylamide, fatty alcohol-polyoxyethylene ether and ethanol.

A preparation method of a bacillus polymyxa water-soluble fertilizer comprises the following steps:

1) weighing: weighing the raw materials according to any one of claims 2 to 5 for use;

2) mixing: uniformly mixing the weighed magnesium sulfate monohydrate, boric acid, zinc sulfate monohydrate, manganese glycinate, iron glycinate, ammonium heptamolybdate, copper glycinate, LTPP03 bacterial powder and surfactant in a stirrer;

3) mixing: stirring ammonium sulfate, nitrogen-potassium compound fertilizer, potassium sulfate and diammonium hydrogen phosphate in a stirrer, adding the amino acid raw powder obtained by weighing in the step 1) and the mixture obtained in the step 2) during stirring, adding anti-caking agent after stirring uniformly, and uniformly mixing to obtain a semi-finished product;

4) packaging: and packaging the semi-finished product to obtain the water-soluble fertilizer.

An application of a water-soluble fertilizer prepared by a preparation method of a bacillus polymyxa water-soluble fertilizer in preventing citrus canker.

According to the technical scheme, compared with the prior art, the amino acid water-soluble fertilizer containing LTPP03 can effectively prevent and treat citrus canker, improve the disease resistance of citrus, and further improve the quality and yield of citrus.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The strain used in the embodiment is bacillus polymyxa, which is preserved in China center for type culture Collection with the preservation address of Wuhan, Wuhan university; the preservation number is CCTCC NO: m2019116, taxonomically named Bacillus polymyxa LTPP03 (Paenibacillus polymyxa LTPP 03), and the nucleotide sequence of 16SrRNA of the LTPP03 of the bacillus polymyxa is shown as SEQ ID No. 1.

EXAMPLE 1 screening of Bacillus polymyxa LTPP03

1) Collecting samples: collecting soil (four pond forest land in Xingning district of Nanning city), and placing the soil in a container;

2) diluting and heating in water bath: placing 10g of soil into 250 ml beaker, adding 90ml of sterile water into the beaker, and oscillating to obtain the product with the concentration of 10^-1The soil dilution A of (1); placing the soil diluent A at 80 deg.CHeating in water bath for 10 min;

3) gradient dilution:

(1) 3 tubes containing 9mL of sterile water were taken and labeled: 10^-2、10^-3And 10^-4；

(2) Shaking the soil diluent A, standing for 30 s, sucking 1mL of soil diluent by a pipette, and adding the soil diluent marked with 10^-2In the test tube, gently and repeatedly blowing and sucking the soil in the test tube for a plurality of times to ensure that the soil is fully and uniformly mixed to obtain a soil diluent B;

(3) similarly, 1mL of the soil diluent B is sucked and added with the serial number of 10^-3Blowing and sucking the mixture evenly in the test tube to obtain a soil diluent C; obtaining soil diluent D by the same method.

4) Coating a flat plate:

respectively sucking A, B, C, D100 ul of soil diluent from low concentration, correspondingly coating the soil diluent on plates which are marked with A, B, C, D and are filled with culture medium, coating 2 plates on each diluent, uniformly coating the dilution by using a coater, and then placing the plates in an environment at 37 ℃ for constant-temperature culture for 20 hours; stopping culturing until bacterial colony grows out of the plate;

5) and (3) streak inoculation: selecting a flat plate with a growing bacterial colony for plate scribing, firstly, carrying out parallel scribing on 3 strips on one side of a plate culture medium, then, rotating a culture dish by an angle of 40 degrees, burning off residues on an inoculating loop, cooling, then, carrying out parallel scribing for the 2 nd time through a 1 st scribing part, carrying out the 3 rd time parallel scribing and the 4 th time parallel scribing through a 2 nd time parallel scribing part by the same method, placing the flat plate after scribing in an incubator for continuous culture until the bacterial colony grows out at the scribing part, and stopping culture;

6) gram staining microscopy:

(1) tabletting: selecting the bacterial colony obtained in the step 5), drying and fixing the bacterial colony on a glass slide to obtain a smear;

(2) primary dyeing: dripping ammonium oxalate crystal violet dye solution on the smear obtained in the step (1), dyeing for 0.5min, pouring out the dye solution, and washing with running water until the purple color fades;

(3) mordant dyeing: washing residual water on the smear obtained in the step (2) by using iodine solution, then covering the coated surface by using the iodine solution with the same concentration for 0.5min, and washing by using running water;

(4) and (3) decoloring: removing residual water of the smear obtained in the step (3), dripping 95% alcohol on the smear, decoloring for 15s, and immediately washing with running water;

(5) counterdyeing: dripping a safranine staining solution on the smear obtained in the step (4), staining for 3min, washing with water, and then blotting with absorbent paper;

(6) microscopic examination: under oil-lens observation, the nucleus was stained blue and the cytoplasm was stained pink.

7) Collecting the strain to obtain the bacillus polymyxa LTPP 03.

Example 2 identification of Bacillus polymyxa LTPP03

The strain of bacillus polymyxa LTPP03 was identified by the chinese type culture collection at 28.02/2019. The identification comprises morphological observation, physiological and biochemical characteristic test and 16SrRNA gene sequence determination and analysis of the strain LTPP03 of the bacillus polymyxa, and the classification status of the strain is determined according to the identification result. The strain is identified as follows: bacillus polymyxa LTPP03 (Paenibacillus polymyxaLTPP 03). The detection results of the physiological and biochemical characteristics are shown in tables 1-2;

TABLE 1 physiological and biochemical characteristics of strain LTPP 03-enzyme activity, carbon source assimilation

Detecting items	The result of the detection
		Reduction of NO3 nitrate to nitrite	+
TRP indoles	-
		GLU acidified glucose	-
ADH arginine double-water enzyme	-
		URE urease	-
ESC hydrolyzed esculin (beta-glucosidase)	+
		GEL hydrolyzed gelatin (protease)	+
PNPG beta-galactosidase	+
		GLU assimilation of glucose	W
ARA assimilation of arabinose	W
		MNE assimilation mannose	+
MAN assimilation of mannitol	+
		NAG-assimilating N-acetyl-glucosamine	-
MAL assimilation maltose	+
		Gnt assimilation gluconate	+
CAP assimilation of capric acid	-
		ADI assimilation of adipic acid	-
MLT assimilation malic acid	-
		CIT assimilation citric acid	W
PAC (poly aluminum chloride) assimilated phenylacetic acid	-

+: positive reaction; -: negative reaction, w is weakly positive reaction

TABLE 2 physiological and biochemical characteristics of Strain LTPP 03-production of acid Using carbon sources

Reagent strip corresponding tube/substrate	The result of the detection	Reagent strip corresponding tube/substrate	The result of the detection
				0 control	-	25 Qiyeling	+
1 Glycerol	+	26 Salol	+
				2 alcohol of erythro tinea	-	27 Cellobiose	+
3D-arabinose	-	28 maltose	+
				4L-arabinose	+	29 lactose	+
5 Ribose	+	30 honey disaccharide	+
				6D-xylose	+	31 sucrose	+
7L-xylose	-	32 trehalose	+
				8 adonitol	-	33 inulin	+
9 beta-methyl-D-xyloside	w	34 Songsansan candy	-
				10 galactose	+	35 Cotton seed candy	+
11 glucose	+	36 starch	+
				12 fructose	+	37 glycogen	+
13 mannose	+	38 xylitol	-
				14 sorbose	-	39 Niaoheng candy	-
15 rhamnose	-	40D-turanose	+
				16 Euonymus alcohol	-	41D-lyxose	-
17 inositol	-	42D-tagatose	-
				18 mannitol	+	43D rock candy	-
19 sorbitol	-	44L rock sugar	-
				20 alpha-methyl-D-mannoside	-	45D-arabinitol	-
21 alpha-methyl-D-glucoside	+	46L-arabinitol	-
				22N-acetyl-glucosamine	-	47 gluconate	-
23 amygdalin	+	482-keto-gluconate	-
				24 bear fruit glycoside	+	495-keto-gluconate	-

+: positive reaction; -: negative reaction;

example 3

1) weighing: weighing 40g of 50 hundred million/g of LTPP03 strain powder, 350g of amino acid raw powder, 100g of ammonium sulfate, 50g of nitrogen-potassium compound fertilizer, 100g of potassium sulfate, 50g of diammonium phosphate, 109g of magnesium sulfate monohydrate, 50g of boric acid, 50g of zinc sulfate monohydrate, 5g of manganese glycinate, 10g of ferric glycinate, 1g of ammonium heptamolybdate, 5g of copper glycinate, 20g of methylene dinaphthalene sodium sulfonate, 40g of sodium lignin sulfonate, 10g of polyacrylamide and 10g of fatty alcohol-polyoxyethylene ether;

2) mixing: uniformly mixing the weighed magnesium sulfate monohydrate, boric acid, zinc sulfate monohydrate, manganese glycinate, iron glycinate, ammonium heptamolybdate, copper glycinate, LTPP03 strain powder, sodium methylene dinaphthalene sulfonate and sodium lignin sulfonate in a stirrer;

3) mixing: stirring ammonium sulfate, a nitrogen-potassium compound fertilizer, potassium sulfate and diammonium hydrogen phosphate in a stirrer, adding the amino acid raw powder obtained in the step 1) and the mixture obtained in the step 2) during stirring, adding polyacrylamide and fatty alcohol-polyoxyethylene ether after uniformly stirring, and uniformly mixing to obtain a semi-finished product;

Example 4

1) weighing: weighing 40g of 50 hundred million/g of LTPP03 strain powder, 350g of amino acid raw powder, 100g of ammonium sulfate, 50g of nitrogen-potassium compound fertilizer, 100g of potassium sulfate, 139g of magnesium sulfate monohydrate, 50g of boric acid, 50g of zinc sulfate monohydrate, 5g of manganese glycinate, 10g of iron glycinate, 1g of ammonium heptamolybdate, 5g of copper glycinate, 15g of methylene dinaphthalene sodium sulfonate, 45g of sodium lignin sulfonate, 5g of polyacrylamide, 7g of fatty alcohol-polyoxyethylene ether and 8g of ethanol;

3) mixing: stirring ammonium sulfate, a nitrogen-potassium compound fertilizer, potassium sulfate and diammonium hydrogen phosphate in a stirrer, adding the amino acid raw powder obtained in the step 1) and the mixture obtained in the step 2) during stirring, adding polyacrylamide, fatty alcohol-polyoxyethylene ether and ethanol after stirring uniformly, and uniformly mixing to obtain a semi-finished product;

Example 4

1) weighing: weighing 40g of 50 hundred million/g of LTPP03 strain powder, 350g of amino acid raw powder, 50g of ammonium sulfate, 125g of potassium sulfate, 75g of diammonium phosphate, 164g of magnesium sulfate monohydrate, 50g of boric acid, 50g of zinc sulfate monohydrate, 5g of manganese glycinate, 10g of iron glycinate, 1g of ammonium heptamolybdate, 5g of copper glycinate, 10g of methylene dinaphthalene sodium sulfonate, 50g of sodium lignin sulfonate, 9g of polyacrylamide and 6g of ethanol;

3) mixing: stirring ammonium sulfate, nitrogen-potassium compound fertilizer, potassium sulfate and diammonium hydrogen phosphate in a stirrer, adding the amino acid raw powder obtained in the step 1) and the mixture obtained in the step 2) during stirring, adding polyacrylamide and ethanol after stirring uniformly, and uniformly mixing to obtain a semi-finished product;

Example 5

1) weighing: weighing 40g of 50 hundred million/g of LTPP03 strain powder, 350g of amino acid raw powder, 150g of potassium sulfate, 100g of diammonium hydrogen phosphate, 169g of magnesium sulfate monohydrate, 50g of boric acid, 50g of zinc sulfate monohydrate, 5g of manganese glycinate, 10g of iron glycinate, 1g of ammonium heptamolybdate, 5g of copper glycinate, 5g of methylene dinaphthalene sodium sulfonate, 55g of sodium lignin sulfonate, 6g of fatty alcohol-polyoxyethylene ether and 4g of ethanol;

3) mixing: stirring ammonium sulfate, a nitrogen-potassium compound fertilizer, potassium sulfate and diammonium hydrogen phosphate in a stirrer, adding the amino acid raw powder obtained in the step 1) and the mixture obtained in the step 2) during stirring, adding fatty alcohol-polyoxyethylene ether and ethanol after stirring uniformly, and uniformly mixing to obtain a semi-finished product;

Example 6

The water-soluble fertilizer prepared in example 3 is sprayed on citrus leaves to verify the effect of the water-soluble fertilizer on preventing and treating citrus canker and the fertilizer efficiency (growth promotion), and the process is as follows:

(1) test preparation

The amino acid-containing water-soluble fertilizer prepared in example 3 comprises an amino acid-containing water-soluble fertilizer (Jinliufeng), 50 hundred million/gram of LTPP03 microbial inoculum and 100 hundred million/gram of Bacillus subtilis microbial inoculum (Deqiang biology).

(2) Design of experiments

The experiment was set to 7 treatments, 4 replicates. The water-soluble fertilizer is set to have 3 concentrations which are respectively 200 times, 250 times and 300 times diluted by adding water, 300 times diluted by adding water to the amino acid-containing water-soluble fertilizer (Jinliufeng), 1000 times diluted by adding water to 50 hundred million/g LTPP03 microbial inoculum and 1000 times diluted by adding water to 100 hundred million/g Bacillus subtilis microbial inoculum (Deqiang biology), and the contrast is clear water. When the young sprout of the mandarin orange is sprayed for 3-5cm, the spraying is carried out for the first time, and the spraying is carried out for 3 times at intervals of 14 days.

(3) Test method

The test site is arranged in the Wuwoo citrus base of Lingwu in Wuming area of Nanning, and the tree age is 4 years. Each treatment cell is 2 Wo citrus trees, the procedure is repeated for 4 times, the trees are randomly arranged in blocks, 1 fruit tree is arranged between each treatment cell, and protection rows are arranged around the treatment cells. The old leaves of the citrus reticulata blanco in the test field have ulcer disease spots, the disease is uniform, and the management of other agricultural works in the test field is consistent.

Each cell was investigated for 2 citrus plants, each plant was investigated for 5 directions in north and south, and each direction was investigated for all leaves on 2 shoots. The leaf grading method comprises the following steps: level 0: no disease; level 1: 1-5 lesions per leaf; and 3, level: 6-10 lesions per leaf; and 5, stage: each leaf lesion is 11-15; and 7, stage: each leaf lesion is 16-20; and 9, stage: more than 21 scabs are generated on each leaf.

And 2 citrus trees are investigated in each cell, 5 directions in east, west, south and north are investigated in each cell, 2 branches are fixedly investigated in each direction, and the number of new shoots, the length of the new shoots and the diameter of the new shoots are investigated 14 days after the last leaf surface spraying.

(4) Calculation method

The prevention and treatment effect is investigated 14 days after the last foliar spraying, and the prevention and treatment effect is calculated according to the disease index by executing the national standard of the people's republic of China, "pesticide field efficacy test criteria (two)".

The drug effect calculation method comprises the following steps:

(5) test results

Table 3 prevention and treatment effects of amino acid-containing water-soluble fertilizer on citrus canker

The test result table 3 shows that compared with clear water control, the water-soluble fertilizer is diluted to 200-fold and 300-fold liquid by adding water, and the control effect on the citrus canker is relatively stable along with the increase of the dilution times; the control effect can reach 71.49-73.65% 14 days after the 3 rd leaf spraying. The control effect of the purchased amino acid water-soluble fertilizer without bacteria is 6.92 percent, while the LTPP03 microbial inoculum is diluted to 1000 times of liquid, the control effect on the citrus reishi canker is 69.67 percent, the bacillus subtilis microbial inoculum (Deqiang) is diluted to 1000 times, and the control effect on the citrus reishi canker is 58.92 percent. From the results, compared with a clear water control and an amino acid water-soluble fertilizer (Jinliufeng), the LTPP03 microbial inoculum has a remarkable prevention effect on the vorax citrus canker; and the amino acid water-soluble fertilizer added with the LTPP03 strain powder is diluted by 200-fold and 300-fold, so that the prevention effect on citrus leaf ulcer is obvious and stable, and the amino acid water-soluble fertilizer containing the LTPP03 strain in example 3 has obvious and stable prevention effect on citrus ulcer.

TABLE 4 influence of amino acid-containing water-soluble fertilizers on shoot number, shoot length, and shoot diameter growth

As can be seen from the results in Table 4, when the woolly oranges begin to perform tip extraction at 3-5cm, the leaf surface sprays on the woolly oranges for preventing canker diseases, the interval is 14 days every time, and 14 days after the last leaf surface spraying, the investigation and comparison are performed on the growth of each treated new tip, the average tip extraction is 4.1-4.3 after the water soluble fertilizer is diluted with 300 times of liquid, the tip extraction length of the new tip is more than 32cm, and the tip extraction is obviously higher than that of clear water control and microbial inoculum treatment; the number of the young sprout leaves and the diameter of the young sprout base part at 1cm are large, and the water soluble fertilizer treatment liquid with the volume of 200 and 300 times is also obviously superior to other treatments.

In conclusion, the water-soluble fertilizer containing the LTPP03 strain can effectively prevent and treat the citrus wadding ulcer disease, has a remarkable effect on trembling and growth promotion, and has double effects of preventing and treating citrus wadding ulcer and promoting growth.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Sequence listing

<110> Guangxi Letu Biotechnology Ltd

<120> bacillus polymyxa water-soluble fertilizer and preparation method and application thereof

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ggcggggtgc tatacatgca agtcgagcgg ggttagttag aagcttgctt ctaactaacc 60

tagcggcgga cgggtgagta acacgtaggc aacctgccca caagacaggg ataactaccg 120

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gcaatctgtc acttgtggat gggcctgcgg cgcattagct agttggtggg gtaaaggcct 240

accaaggcga cgatgcgtag ccgacctgag agggtgatcg gccacactgg gactgagaca 300

cggcccagac tcctacggga ggcagcagta gggaatcttc cgcaatgggc gaaagcctga 360

cggagcaacg ccgcgtgagt gatgaaggtt ttcggatcgt aaagctctgt tgccagggaa 420

gaacgctttg tagagtaact gctcttgaag tgacggtacc tgagaagaaa gccccggcta 480

actacgtgcc agcagccgcg gtaatacgta gggggcaagc gttgtccgga attattgggc 540

gtaaagcgcg cgcaggcggc tctttaagtc tggtgtttaa tcccgaggct caacttcggg 600

tcgcactgga aactggggag cttgagtgca gaagaggaga gtggaattcc acgtgtagcg 660

gtgaaatgcg tagagatgtg gaggaacacc agtggcgaag gcgactctct gggctgtaac 720

tgacgctgag gcgcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 780

cgtaaacgat gaatgctagg tgttaggggt ttcgataccc ttggtgccga agttaacaca 840

ttaagcattc cgcctgggga gtacggtcgc aagactgaaa ctcaaaggaa ttgacgggga 900

cccgcacaag cagtggagta tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 960

cttgacatcc ctttgaccgg tctagagata gatctttcct tcgggacaga ggagacaggt 1020

ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1080

aacccttatg cttagttgcc agcaggtcaa gctgggcact ctaagcagac tgccggtgac 1140

aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac 1200

acgtactaca atggccggta caacgggaag cgaaggagcg atctggagcc aatcctagaa 1260

aagccggtct cagttcggat tgtaggctgc aactcgccta catgaagtcg gaattgctag 1320

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aagt 1444

Claims

1. The water-soluble fertilizer for bacillus polymyxa is characterized by comprising the following components: bacillus polymyxa; the bacillus polymyxa is preserved in China center for type culture Collection with the preservation number of CCTCC No: m2019116, 16srRNA sequence is shown in SEQ ID No. 1.

2. The bacillus polymyxa water-soluble fertilizer as defined in claim 1, further comprising: 350g of amino acid raw powder, 0-100g of ammonium sulfate, 0-50g of nitrogen-potassium compound fertilizer, 100g of potassium sulfate, 0-100g of diammonium hydrogen phosphate, 109g of magnesium sulfate monohydrate, 50g of boric acid, 50g of zinc sulfate monohydrate, 5g of manganese glycinate, 10g of iron glycinate, 1g of ammonium heptamolybdate, 5g of copper glycinate, 60g of surfactant and 0-20g of anti-caking powder.

3. The water-soluble fertilizer for bacillus polymyxa according to claim 2, wherein the weight of the LTPP03 bacterial powder in the water-soluble fertilizer is 40g, and the content of LTPP03 bacterial cells in each gram of bacterial powder is 50 hundred million/g.

4. The bacillus polymyxa water-soluble fertilizer as claimed in claim 2, wherein the surfactant comprises: the sodium methylene dinaphthalene sulfonate and the sodium lignin sulfonate are mixed according to the mass ratio of 1 (2-11).

5. The bacillus polymyxa water-soluble fertilizer as set forth in claim 2, wherein the anti-caking agent comprises: any two or three of polyacrylamide, fatty alcohol-polyoxyethylene ether and ethanol.

6. The preparation method of the bacillus polymyxa water-soluble fertilizer is characterized by comprising the following steps of:

7. The application of the water-soluble fertilizer prepared by the preparation method of the bacillus polymyxa water-soluble fertilizer according to claim 6 in preventing citrus canker.