CN111504990B - Environment-friendly gas absorption detection plate special for microplate reader and use method thereof - Google Patents
Environment-friendly gas absorption detection plate special for microplate reader and use method thereof Download PDFInfo
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- CN111504990B CN111504990B CN202010352968.6A CN202010352968A CN111504990B CN 111504990 B CN111504990 B CN 111504990B CN 202010352968 A CN202010352968 A CN 202010352968A CN 111504990 B CN111504990 B CN 111504990B
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- 238000010521 absorption reaction Methods 0.000 title claims abstract description 112
- 238000001514 detection method Methods 0.000 title claims abstract description 101
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000002372 labelling Methods 0.000 claims abstract description 8
- 239000000376 reactant Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 239000011159 matrix material Substances 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims description 25
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 16
- 239000000741 silica gel Substances 0.000 claims description 16
- 229910002027 silica gel Inorganic materials 0.000 claims description 16
- 238000007689 inspection Methods 0.000 claims description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 238000009826 distribution Methods 0.000 claims description 3
- 238000005192 partition Methods 0.000 claims description 3
- 241000784303 Ochrosia compta Species 0.000 claims description 2
- 239000002250 absorbent Substances 0.000 abstract description 9
- 230000002745 absorbent Effects 0.000 abstract description 9
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 238000007711 solidification Methods 0.000 abstract description 2
- 230000008023 solidification Effects 0.000 abstract description 2
- 230000001012 protector Effects 0.000 abstract 2
- 239000007789 gas Substances 0.000 description 72
- 238000004458 analytical method Methods 0.000 description 6
- 230000001681 protective effect Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000012495 reaction gas Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/783—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour for analysing gases
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- Biochemistry (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides an environment-friendly gas absorption detection plate special for an enzyme-labeling instrument and a using method thereof, and relates to the field of gas generation absorption detection. The middle gas absorption detection unit is arranged in a matrix form through the bottom of the special plate, the upper part edge protector is fixedly connected to the periphery of the upper part of the bottom of the special plate, and the bottom edge protector is fixedly connected to the periphery of the lower part of the bottom of the special plate. The advantages are that: corresponding gas absorption liquid which does not need to be solidified is directly added to absorb gas, and the gas is directly analyzed and detected by a microplate reader after color reaction, so that the operation is convenient and simple, the solidification process of a gas absorbent is avoided, the gas absorbent is rightly placed above a reactant for culture and absorption, and the inconvenience of mirror image data when the gas is reversely cultured and absorbed is avoided; 96 samples are absorbed and measured at most simultaneously, so that the detection rate is greatly improved, and a large amount of test time can be saved.
Description
Technical Field
The invention relates to the field of gas production absorption detection in the aspects of ecology, environment, life science and agriculture, in particular to an environment-friendly gas absorption detection plate special for an microplate reader and a use method thereof.
Background
In many researches in the fields of ecology, environment, life science and agriculture, absorption and detection of gas generated by organisms can be involved, but the existing related technologies are complex to operate, can be completed only by combining various devices, vessels and pipelines, and need various consumables and reagents in large amount, and many test reagents are toxic and harmful to the environment and the organisms, so that the corresponding test cost is high, and the environment is not friendly.
The enzyme-labeling instrument and the spectrophotometer are common analysis and measurement tools in a laboratory, and although the determination principles of the enzyme-labeling instrument and the spectrophotometer are the same, when the spectrophotometer is used for analysis and measurement, the workload is large, the operation is complicated, the time consumption is long, the reagent consumption is large, and trace substances are difficult to detect; when the enzyme-linked immunosorbent assay device is used for analysis, the single-time treatment of the device is large in sample amount, analysis time is saved, the amount of required samples and reagents is small, analysis cost is saved, harmful substance emission is reduced, the operation of the device is simple, repeatability is good, detection speed is high, and efficiency is high, so that most conditional laboratories use the enzyme-linked immunosorbent assay device for analysis and detection.
However, for some detections with special requirements, such as absorption detection of reaction gas, the conventional microplate reader detection plate cannot be directly performed, and can be completed after complicated treatment, such as solidifying a gas absorbent, fixing the gas absorbent in the micropores of the conventional microplate reader detection plate, reversely buckling the gas absorbent on the top of a deep-well culture plate used in cooperation to absorb corresponding gas, and then detecting and analyzing by using the microplate reader. The process needs a special curing agent, the experimental steps are complicated, the operation is complicated, and a large number of uncertain factors are added to the accuracy of the experimental result.
Disclosure of Invention
The invention provides an environment-friendly gas absorption detection plate special for an microplate reader and a using method thereof, and aims to solve the problems that the existing conventional detection plate of the microplate reader cannot be directly used for detecting with special requirements such as absorption detection of reaction gas, can be completed after complicated treatment, needs a special curing agent in the process, has complicated experimental steps and complicated operation, and adds a large number of uncertain factors to the accuracy of experimental results.
The technical scheme adopted by the invention is as follows: the method comprises the following steps: the gas absorption detection unit is arranged in a matrix form through the special plate bottom, the upper protection edge is fixedly connected with the periphery of the upper part of the special plate bottom, and the lower protection edge is fixedly connected with the bottom protection edge;
the gas absorption detection unit includes: gaseous absorption inspection hole outer bottom cleaning protection platform, special-purpose board end, air vent outer wall, air vent, the intercommunication groove of air vent and gaseous absorption inspection hole, the outer wall of gaseous absorption inspection hole, the mesophragma of air vent and gaseous absorption inspection hole, wherein: the vent hole is a small hole which is positioned beside the gas absorption detection hole and is communicated up and down, the outer wall of the vent hole is provided with an annular gas absorption detection hole outer bottom cleaning protection platform outside the bottom of the special plate, the communicating groove is a groove for communicating the gas absorption detection hole and the vent hole, the upper outer edge of the communicating groove is flush with the outer edge of the hole opening of the gas absorption detection hole, and the bottom of the communicating groove is the middle partition of the vent hole and the gas absorption detection hole.
The number of the gas absorption detection units is 1-96.
Inner diameter of the vent hole
Is not less than 1mm, and the bottom height h of the special plate with the lower part protruding out1Is 1-3 mm.
The height h of the outer bottom cleaning and protecting platform of the gas absorption detection hole2Is 0.5 mm.
The gas absorption detection hole is of a transparent flat bottom type and is high h3The thickness is 10mm, the position distribution is consistent with that of a universal enzyme label plate, and the direct reading value detection by an enzyme label instrument is convenient.
The height h of the communicating groove4Not less than 3mm, groove width and vent hole inner diameter
Are equal.
The air vent of the gas absorption detection unit is connected with a sealing hole in the plate bottom connection sealing silica gel pad in an inserting mode, the position of the sealing hole in the plate bottom connection sealing silica gel pad is consistent with the position of the air vent of the gas absorption detection unit, and the inner diameter of the sealing hole is smaller than the outer diameter of the lower end outer edge of the air vent.
The upper part of the gas absorption detection unit is in contact connection with the lining orifice sealing silica gel pad of the special plate top cover.
An application method of an environment-friendly gas absorption detection plate special for an enzyme-labeling instrument; comprises the following steps:
(1) placing reactant B in a reactor such that the maximum volume of reactant per pore does not exceed 1/3 of the pore volume;
(2) the reactor is connected with a gas absorption detection unit through a plate bottom connecting sealing silica gel pad,
(3) rapidly adding a gas absorption detection agent A into the gas absorption detection hole by using a multichannel micropipette, wherein the liquid level of the gas absorption detection agent is lower than the middle between the vent hole and the gas absorption detection hole;
(4) immediately fastening the special plate top cover with the hole sealing silica gel pad, and clamping by using a tool;
(5) placing the device in a place with proper conditions, culturing and absorbing for a certain time;
(6) taking down the special gas absorption detection plate, and detecting with an enzyme-labeling instrument directly or after adding a color reagent.
The reactor is a commercial 96-hole deep-hole plate.
The invention provides a gas absorption and detection related assembly integrated into a gas absorption and detection unit, wherein each plate is provided with a plurality of gas absorption and detection units arranged in a matrix array, and the gas absorption and detection units are matched with corresponding deep-hole culture plates and corresponding accessories for use, so that the gas absorption and detection device has the following advantages:
(1) corresponding gas absorption liquid which does not need to be solidified is directly added to absorb gas, and the gas is directly analyzed and detected by a microplate reader after color reaction, so that the operation is convenient and simple, the solidification process of a gas absorbent is avoided, the gas absorbent is rightly placed above a reactant for culture and absorption, and the inconvenience of mirror image data when the gas is reversely cultured and absorbed is avoided;
(2) because the gas absorbent does not need to be solidified, the data is more accurate when the microplate reader detects the gas absorbent;
(3) the required reagent amount and sample amount are small, so that a large amount of test cost can be saved, and the emission of harmful substances can be reduced;
(4) 96 samples can be absorbed and measured at most simultaneously, so that the detection rate is greatly improved, and a large amount of test time can be saved.
Drawings
FIG. 1 is an exploded view of the structure of the invention;
FIG. 2 is a schematic view of the structure of an array of gas absorption detecting units, an upper protective edge, and a lower protective edge of a plate according to the present invention;
FIG. 3 is a schematic view of the structure of the gas absorption detecting unit of the present invention;
FIG. 4 is a bottom view of the gas absorption detection unit of the present invention;
FIG. 5 is a cross-sectional view of a gas absorption detection unit of the present invention;
FIG. 6 is a cross-sectional view of a gas absorption detection unit and its accessory assembly of the present invention;
fig. 7 is a use state reference diagram of the present invention.
Detailed Description
The method comprises the following steps: the gas absorption detection device comprises a gas absorption detection unit 1, an upper protective edge 2 and a plate bottom protective edge 3, wherein the gas absorption detection unit 1 is arranged in a matrix form through a special plate bottom 1.2, the upper protective edge 2 is fixedly connected to the periphery of the upper part of the special plate bottom 1.2, and the lower protective edge 3 is fixedly connected to the periphery of the lower part of the special plate bottom 1.2;
the gas absorption detection unit 1 includes: gaseous absorption inspection hole outer bottom clean protection platform 1.1, special-purpose board end 1.2, air vent outer wall 1.3, air vent 1.4, air vent and gaseous absorption inspection hole's intercommunication groove 1.5, gaseous absorption inspection hole's outer wall 1.6, air vent and gaseous absorption inspection hole's well 1.7, gaseous absorption inspection hole 1.8, wherein: the vent hole 1.4 is a small hole which is positioned beside the gas absorption detection hole 1.8 and is through up and down, the outer wall 1.3 of the vent hole is provided with an annular gas absorption detection hole outer bottom cleaning and protecting platform 1.1 outside the lower part 1.2 of the special plate bottom, the communicating groove 1.5 is a groove for communicating the gas absorption detection hole 1.8 with the vent hole 1.4, the outer edge of the upper part of the groove is flush with the outer edge of the hole opening of the gas absorption detection hole 1.8, and the bottom of the groove is a middle partition 1.7 of the vent hole and the gas absorption detection hole.
The number of the gas absorption detection units 1 is 1-96.
Inner diameter of the vent hole 1.4
Is not less than 1mm, and the bottom of the special plate with the lower part protruding is 12 h1Is 1-3 mm.
The outer bottom cleaning and protecting platform of the gas absorption detection hole has a height of 1.1 h20.5mm, make the gas absorption inspection hole bottom outside not touch sealing silica gel pad 5 to guarantee the cleanness in the gas absorption inspection hole 1.8 bottom outside, do not influence the degree of accuracy of experimental result.
The gas absorption detection hole 1.8 is a transparent flat bottom type, and the height h of the gas absorption detection hole310mm, the position distribution is consistent with that of a universal enzyme label plate, and the direct reading value detection by an enzyme label instrument is convenient;
the communicating groove is 1.5 h high4Is not lowAt 3mm, the width of the groove and the inner diameter of the vent hole
Equal;
the air vent 1.4 of the gas absorption detection unit 1 is connected with a sealing hole 5.1 on a plate bottom connection sealing silica gel pad 5 in an inserting mode, the position of the sealing hole 5.1 on the plate bottom connection sealing silica gel pad 5 is consistent with the position of the air vent 1.4 of the gas absorption detection unit 1, and the inner diameter of the sealing hole 5.1 is smaller than the outer diameter of the outer edge of the lower end of the air vent so as to increase the connection air tightness.
The upper part of the gas absorption detection unit 1 is in contact connection with a lining orifice sealing silica gel pad 4.1 of the special plate top cover 4.
An application method of an environment-friendly gas absorption detection plate special for an enzyme-labeling instrument; comprises the following steps:
(1) placing reactant B in a reactor such that the maximum volume of reactant per pore does not exceed 1/3 of the pore volume;
(2) the reactor is connected with the gas absorption detection unit 1 through a plate bottom connecting sealing silica gel pad 5,
(3) quickly adding a gas absorption detection agent A into a gas absorption detection hole 1.8 by using a multichannel micropipette, wherein the liquid level of the gas absorption detection agent is lower than the middle between a vent hole and the gas absorption detection hole by 1.7;
(4) immediately fastening the special plate top cover 4 with the hole sealing silica gel pad 4.1, and clamping by using a tool;
(5) placing the device in a place with proper conditions, culturing and absorbing for a certain time;
(6) taking down the special gas absorption detection plate, and detecting with an enzyme-labeling instrument directly or after adding a color reagent.
The reactor is a commercial 96-hole deep-hole plate.
Claims (10)
1. The utility model provides a special gas absorption pick-up plate of ELIASA of environment-friendly which characterized in that: the method comprises the following steps: the gas absorption detection unit is arranged in a matrix form through the special plate bottom, the upper protection edge is fixedly connected with the periphery of the upper part of the special plate bottom, and the lower protection edge is fixedly connected with the bottom protection edge;
the gas absorption detection unit includes: gaseous absorption inspection hole outer bottom cleaning protection platform, special-purpose board end, air vent outer wall, air vent, the intercommunication groove of air vent and gaseous absorption inspection hole, the outer wall of gaseous absorption inspection hole, the mesophragma of air vent and gaseous absorption inspection hole, wherein: the vent hole is a small hole which is positioned beside the gas absorption detection hole and is communicated up and down, the outer wall of the vent hole is provided with an annular gas absorption detection hole outer bottom cleaning protection platform outside the bottom of the special plate, the communicating groove is a groove for communicating the gas absorption detection hole and the vent hole, the upper outer edge of the communicating groove is flush with the outer edge of the hole opening of the gas absorption detection hole, and the bottom of the communicating groove is the middle partition of the vent hole and the gas absorption detection hole.
2. The gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, wherein: the number of the gas absorption detection units is 1-96.
3. The gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, wherein: inner diameter of the vent hole
Is not less than 1mm, and the bottom height h of the special plate with the lower part protruding out1Is 1-3 mm.
4. The gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, wherein: the height h of the outer bottom cleaning and protecting platform of the gas absorption detection hole2Is 0.5 mm.
5. The gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, wherein: the gas absorption detection hole is of a transparent flat bottom type and is high h3The thickness is 10mm, the position distribution is consistent with that of a universal enzyme label plate, and the direct reading value detection by an enzyme label instrument is convenient.
6. The gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, wherein: the height h of the communicating groove4Not less than 3mm, groove width and vent hole inner diameter
Are equal.
7. The gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, wherein: the air vent of the gas absorption detection unit is connected with a sealing hole in the plate bottom connection sealing silica gel pad in an inserting mode, the position of the sealing hole in the plate bottom connection sealing silica gel pad is consistent with the position of the air vent of the gas absorption detection unit, and the inner diameter of the sealing hole is smaller than the outer diameter of the lower end outer edge of the air vent.
8. The gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, wherein: the upper part of the gas absorption detection unit is in contact connection with the lining orifice sealing silica gel pad of the special plate top cover.
9. The method for using the gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 1, characterized by comprising the following steps:
(1) placing reactant B in a reactor such that the maximum volume of reactant per pore does not exceed 1/3 of the pore volume;
(2) the reactor is connected with a gas absorption detection unit through a plate bottom connecting sealing silica gel pad,
(3) rapidly adding a gas absorption detection agent A into the gas absorption detection hole by using a multichannel micropipette, wherein the liquid level of the gas absorption detection agent is lower than the middle between the vent hole and the gas absorption detection hole;
(4) immediately fastening the special plate top cover with the hole sealing silica gel pad, and clamping by using a tool;
(5) placing the device in a place with proper conditions, culturing and absorbing for a certain time;
(6) taking down the special gas absorption detection plate, and detecting with an enzyme-labeling instrument directly or after adding a color reagent.
10. The method for using the gas absorption detection plate special for the environment-friendly microplate reader as claimed in claim 9, wherein the reactor is a commercially available 96-well deep-well plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010352968.6A CN111504990B (en) | 2020-04-28 | 2020-04-28 | Environment-friendly gas absorption detection plate special for microplate reader and use method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010352968.6A CN111504990B (en) | 2020-04-28 | 2020-04-28 | Environment-friendly gas absorption detection plate special for microplate reader and use method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN111504990A CN111504990A (en) | 2020-08-07 |
| CN111504990B true CN111504990B (en) | 2022-02-11 |
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| CN202010352968.6A Expired - Fee Related CN111504990B (en) | 2020-04-28 | 2020-04-28 | Environment-friendly gas absorption detection plate special for microplate reader and use method thereof |
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| EP2040071A2 (en) * | 2007-09-18 | 2009-03-25 | HY-Energy LLP | Gas sorption tester for rapid screening of multiple samples |
| CN202330210U (en) * | 2011-11-30 | 2012-07-11 | 神华集团有限责任公司 | Gas testing device |
| CN105866116A (en) * | 2016-05-27 | 2016-08-17 | 天津果实科技有限公司 | Device for detecting test paper one on one by virtue of color sensors |
| CN107340288A (en) * | 2016-12-31 | 2017-11-10 | 青岛大学 | A kind of equipment for detecting air formaldehyde concentration |
| CN109490288A (en) * | 2018-11-15 | 2019-03-19 | 湖北德立森科技有限公司 | A kind of urinalysis device that can accurately detect |
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| US20060105391A1 (en) * | 2004-11-12 | 2006-05-18 | Promega Corporation | Device and method for separating molecules |
| US7705312B2 (en) * | 2005-12-08 | 2010-04-27 | The Vision Group, Inc. | Multi-gas sensor |
| CH703700A2 (en) * | 2010-09-08 | 2012-03-15 | Tecan Trading Ag | Control of the atmosphere in microplate readers. |
| CN103323496B (en) * | 2013-05-24 | 2018-12-18 | 中国人民解放军第二五一医院 | High-throughput percutaneous corrosion and absorption detecting plate |
-
2020
- 2020-04-28 CN CN202010352968.6A patent/CN111504990B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1429915A (en) * | 2003-01-30 | 2003-07-16 | 崔克敏 | Tumour cell drug sensitive testing method and its testing plate |
| CN201158658Y (en) * | 2007-09-17 | 2008-12-03 | 上海中正慧东医疗技术有限公司 | Drop composition board for microbe drug sensitivity experiment |
| EP2040071A2 (en) * | 2007-09-18 | 2009-03-25 | HY-Energy LLP | Gas sorption tester for rapid screening of multiple samples |
| CN202330210U (en) * | 2011-11-30 | 2012-07-11 | 神华集团有限责任公司 | Gas testing device |
| CN105866116A (en) * | 2016-05-27 | 2016-08-17 | 天津果实科技有限公司 | Device for detecting test paper one on one by virtue of color sensors |
| CN107340288A (en) * | 2016-12-31 | 2017-11-10 | 青岛大学 | A kind of equipment for detecting air formaldehyde concentration |
| CN109490288A (en) * | 2018-11-15 | 2019-03-19 | 湖北德立森科技有限公司 | A kind of urinalysis device that can accurately detect |
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