CN113189075B - ATP-based cold chain food outer package cleanliness detection method - Google Patents
ATP-based cold chain food outer package cleanliness detection method Download PDFInfo
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- CN113189075B CN113189075B CN202110517302.6A CN202110517302A CN113189075B CN 113189075 B CN113189075 B CN 113189075B CN 202110517302 A CN202110517302 A CN 202110517302A CN 113189075 B CN113189075 B CN 113189075B
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- 238000001514 detection method Methods 0.000 title claims abstract description 49
- 230000003749 cleanliness Effects 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 84
- 238000005070 sampling Methods 0.000 claims abstract description 42
- 108091006112 ATPases Proteins 0.000 claims abstract description 23
- 102000057290 Adenosine Triphosphatases Human genes 0.000 claims abstract description 23
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 22
- 230000001580 bacterial effect Effects 0.000 claims abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 5
- 238000003860 storage Methods 0.000 claims description 43
- 238000007789 sealing Methods 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 14
- 238000003825 pressing Methods 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 9
- 239000011550 stock solution Substances 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 4
- 238000004806 packaging method and process Methods 0.000 claims description 4
- 238000001179 sorption measurement Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 49
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 49
- 230000000813 microbial effect Effects 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 2
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 2
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 2
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000254158 Lampyridae Species 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N2001/028—Sampling from a surface, swabbing, vaporising
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cold chain food outer package cleanliness detection method based on ATP, which comprises the following steps: firstly, taking a plurality of ATP swabs, wherein in every two ATP swabs, one ATP swab is filled with ATPase liquid and reaction liquid, and the other ATP swab is filled with ATPase liquid, selective bacterial culture liquid and reaction liquid; step two, placing a plurality of ATP swabs into the tube seat, and enabling a plurality of ejector pins on the tube seat to extend into the swabs respectively; step three, parallelly sampling two sampling tubes filled with different reagents; pushing the push plate downwards to enable the sample to be contacted with the ATPase liquid, the selective bacterial culture liquid and the reaction liquid in sequence; and step seven, detecting and comparing to obtain the cleanliness of the cold chain food outer package. The invention has the characteristics of simple and convenient repeated detection operation, accurate and comprehensive detection and capability of detecting specific bacteria.
Description
Technical Field
The invention relates to a cleanliness detection method, in particular to an ATP-based cold chain food outer package cleanliness detection method.
Background
Adenosine Triphosphate (ATP) is a direct source of energy of all living beings and is commonly existed in animal and plant cells, microorganisms and food residues, an ATP fluorescence detection method is a rapid detection technology developed according to a firefly luminescence principle, luciferase can catalyze luciferin and ATP to react to form luciferin and emit fluorescence, the emitted fluorescence intensity is in direct proportion to the number of microorganisms, and the degree of microbial contamination of an object to be detected can be obtained by testing the fluorescence intensity, so that the detection ATP can be used as an visual index for judging whether cold chain food outer package is clean or not.
The existing ATP detection method generally adopts an ATP swab to directly sample and detect the surface of an object to be detected, has a narrow one-time detection range, has insufficient comprehensive detection data, needs to perform multiple times of one-to-one tests and reactions if multiple times of detection are needed, and is troublesome and time-consuming to operate; in addition, in the sampled sample, besides ATP in bacteria, some free ATP can interfere with detection data of actual bacteria, so that detection accuracy is affected; and after the reaction, only the total bacterial amount can be detected, and whether some special disease bacteria exist can not be known.
Therefore, the conventional ATP detection method has the characteristics of troublesome detection operation for a plurality of times, to-be-improved detection accuracy and incapability of detecting specific bacteria.
Disclosure of Invention
The invention aims to provide an ATP-based cold chain food outer package cleanliness detection method. The invention has the characteristics of simple and convenient repeated detection operation, accurate and comprehensive detection and capability of detecting specific bacteria.
The technical scheme of the invention is as follows: a cold chain food outer package cleanliness detection method based on ATP comprises the following steps:
firstly, taking a plurality of ATP swabs, wherein in every two ATP swabs, one ATP swab is filled with ATPase liquid and reaction liquid, and the other ATP swab is filled with ATPase liquid, selective bacterial culture liquid and reaction liquid;
step two, placing a plurality of ATP swabs into the tube seat, and enabling a plurality of ejector pins on the tube seat to extend into the swabs respectively;
step three, parallelly sampling two sampling tubes filled with different reagents, and reinstalling the sampling tubes on a swab after sampling is completed;
pushing the push plate downwards to enable the ejector pin to eject a cavity for storing the ATPase liquid, wherein the ATPase liquid contacts with the sample to process unnecessary ATP;
step five, pushing the push plate continuously to enable the ejector pin to eject the cavity for storing the selective bacterial culture solution, enabling the selective bacterial culture solution to contact with the sample, and culturing and amplifying bacteria;
step six, pushing the push plate continuously to enable the thimble to push the cavity for storing the reaction liquid, and enabling the reaction liquid to contact with the sample for reaction;
and step seven, placing the reacted ATP swab into a detection bin of an ATP detection instrument for detection and comparison, and obtaining the cleanliness of the cold chain food outer package.
In the above method for detecting cleanliness of cold chain food outer package based on ATP, the total sampling area of the plurality of ATP swabs is the cold chain food outer package area, and the sampling mode is to wipe the surface of the cold chain food outer package in a wave-shaped rolling manner.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, the swab comprises a liquid storage pipe, wherein a first liquid storage cavity, a second liquid storage cavity and a third liquid storage cavity which are isolated from each other are arranged in the liquid storage pipe; the flow tube is arranged below the liquid storage tube, the sealing element is arranged between the liquid storage tube and the flow tube, the sampling tube is arranged below the flow tube, and the reaction tube is sleeved outside the sampling tube.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, a push plate which moves up and down is arranged above the tube seat, a plurality of piston plates which correspond to the swabs one by one are arranged at the bottom of the push plate, ejector pins which extend into the first liquid storage cavity, the second liquid storage cavity and the third liquid storage cavity respectively are arranged at the bottom of the piston plates, and the lengths of the three ejector pins are different.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, the tube seat is provided with the sliding groove, the push plate is provided with the sliding rod extending into the sliding groove, and one side of the sliding groove is provided with three scale marks for marking the sliding position.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, a groove is formed in the bottom of the push plate, a first absorption part is arranged at the top of the groove, and a second absorption part which is absorbed with the first absorption part is arranged on the top surface of the piston plate.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, the sealing piece comprises an annular pressing plate, a supporting plate is arranged below the pressing plate, a sealing film is arranged between the pressing plate and the supporting plate, top fragments respectively positioned in the first liquid storage cavity, the second liquid storage cavity and the third liquid storage cavity are arranged on the sealing film, cross-shaped cutting grooves corresponding to the ejector pins are formed in the top fragments, and liquid outlet holes corresponding to the top fragments are formed in the supporting plate.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, the sealing piece further comprises a plurality of U-shaped elastic cards, the lower ends of the elastic cards are rotationally connected with the edges of the supporting plates, and the upper ends of the elastic cards are buckled with the edges of the pressing plates.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, the flow tube is in a conical shape with a large upper part and a small lower part, and the sampling tube is detachably connected with the flow tube through the clamping assembly.
In the method for detecting the cleanliness of the cold chain food outer package based on ATP, the clamping assembly comprises a fixing ring, a slot for inserting the upper end of the sampling tube is arranged at the bottom of the fixing ring, a plurality of fixing parts are arranged on the circumference of the inner wall of the slot, an embedded groove is formed in one side of the fixing seat, and a clamping block inserted into the embedded groove is arranged on the outer wall of the sampling tube.
Compared with the prior art, the detection method of the invention has the advantages that the ATPase liquid and the reaction liquid are respectively filled in one swab, the ATPase liquid, the selective bacterial culture liquid and the reaction liquid are respectively filled in the other swab, after the swab is sampled, the ATPase liquid is utilized by the one swab to treat unnecessary interference ATP, the detection accuracy is improved, and the reaction detection is carried out by utilizing the swab of the reaction liquid to obtain the microbial biomass; the other swab firstly utilizes ATPase liquid to treat unnecessary interfering ATP, then utilizes selective bacterial culture liquid to culture and amplify specific bacteria to be detected, improves detection pertinence, then utilizes reaction liquid to perform reaction detection to obtain the amplified microbial biomass, and compares the two detected microbial biomass to obtain whether a specific bacteria exists on the cold chain food outer package;
the swab capable of containing multiple liquids and the matched tube seat capable of containing multiple swabs are arranged, and the plurality of ejector pins can be pushed to respectively and one by one break the sealing elements in the corresponding swabs by pushing the push plate, so that different liquids are sequentially contacted and reacted with the sampling samples, the operation is simple, the detection time is saved, and the detection efficiency is greatly improved; and a plurality of swabs can be taken to detect different positions of the sample, so that the detection accuracy and the comprehensiveness are improved.
Therefore, the invention has the characteristics of simple and convenient repeated detection operation, accurate and comprehensive detection and capability of detecting specific bacteria.
Drawings
FIG. 1 is a schematic view of a swab construction of the present invention;
FIG. 2 is a schematic structural view of a seal;
fig. 3 is a schematic structural view of the engaging component.
The marks in the drawings are: 1. a liquid storage tube; 11. a first reservoir; 12. a second reservoir; 13. a third reservoir; 2. a flow tube; 21. a sampling tube; 22. a reaction tube; 3. a seal; 31. a pressing plate; 32. a support plate; 33. a sealing film; 34. a top breaker; 35. a cross-shaped slot; 36. a liquid outlet hole; 37. an elastic card; 38. a seal ring; 4. a tube seat; 41. a swab chamber; 42. a push plate; 421. a groove; 422. a first absorbent member; 43. a piston plate; 431. a second adsorption member; 44. a thimble; 45. a sliding groove; 46. a slide bar; 47. scale marks; 5. a clamping assembly; 51. a fixing ring; 52. slotting; 53. a fixing seat; 54. and (5) clamping blocks.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to be limiting.
Examples:
as shown in fig. 1 to 3, the method for detecting the cleanliness of the outer package of the cold chain food based on the ATP comprises the following steps:
firstly, taking a plurality of ATP swabs, wherein one ATP swab is filled with ATPase liquid and reaction liquid, and the other ATP swab is filled with ATPase liquid, selective bacterial culture liquid and reaction liquid, and the ATPase liquid, the selective bacterial culture liquid and the reaction liquid are all stored in an isolated manner;
step two, placing a plurality of ATP swabs into a swab cavity 41 of the tube seat 4 one by one, and enabling a plurality of ejector pins 44 on a push plate 42 which can move up and down on the tube seat 4 to extend into the swabs respectively;
step three, removing the sampling tube 21 from the ATP swab, enabling two sampling tubes 21 filled with different reagents to sample in parallel, and reinstalling the sampling tubes 21 on the swab after sampling is completed;
pushing the push plate 42 downwards to enable the ejector pins 44 to firstly break the chambers for storing the ATPase liquid in the plurality of ATP swabs, and enabling the ATPase liquid to contact with the sample to treat unnecessary ATP;
step five, pushing the push plate 42 continuously to enable the ejector pins 44 to eject the chambers for storing the selective bacterial culture solution in the plurality of ATP swabs, and enabling the selective bacterial culture solution to contact with the sample for culturing and amplifying bacteria;
step six, pushing the push plate 42 continuously to enable the ejector pins 44 to finally eject chambers for storing reaction liquid in the plurality of ATP swabs, enabling the reaction liquid to contact with the sample for reaction, and utilizing energy released by ATP to emit fluorescence, wherein the luminous intensity is in direct proportion to the quantity of ATP;
and step seven, placing the reacted ATP swab into a detection bin of an ATP detection instrument for detection, reading out the fluorescence intensity by the instrument, calculating the thallus content range in the sample, obtaining the cleanliness of the cold chain food outer package, and comparing the two swab measurement results filled with different reagents to obtain whether specific bacteria corresponding to the selective bacteria culture solution exist in the cold chain food outer package.
The total sampling area of the ATP swabs is the cold chain food outer packaging area, and the sampling mode is to wipe the surface of the cold chain food outer packaging in a wave rolling mode.
The swab comprises a liquid storage tube 1, wherein a first liquid storage cavity 11, a second liquid storage cavity 12 and a third liquid storage cavity 13 which are isolated from each other are arranged in the liquid storage tube 1; the flow tube 2 is arranged below the liquid storage tube 1, the sealing element 3 is arranged between the liquid storage tube 1 and the flow tube 2, the sampling tube 21 is arranged below the flow tube 2, and the reaction tube 22 is sleeved outside the sampling tube 21. The first liquid storage cavity 11 is filled with ATPase liquid, the second liquid storage cavity 12 is filled with selective bacterial culture liquid, and the third liquid storage cavity 13 is filled with reaction liquid.
The top of tube socket 4 is equipped with the push pedal 42 of upper and lower activity, and the bottom of push pedal 42 is equipped with a plurality of piston boards 43 that correspond with the swab one by one, and the bottom of piston board 43 is equipped with the thimble 44 that stretches into respectively in first stock solution chamber 11, second stock solution chamber 12 and the third stock solution chamber 13, and the length of three thimble 44 is different.
The tube seat 4 is provided with a sliding groove 45, the push plate 42 is provided with a sliding rod 46 extending into the sliding groove 45, and one side of the sliding groove 45 is provided with three graduation marks 47 for marking the sliding position. When the sliding rod 46 moves to the uppermost scale line 47, the longest thimble 44 breaks the first liquid storage cavity 11 to release ATPase liquid; when the sliding rod 46 moves to the middle scale line 47, the thimble 44 with the middle length breaks the second liquid storage cavity 12 to release the selective bacterial culture solution, and when the sliding rod 46 moves to the lowest scale line 47, the shortest thimble 44 breaks the third liquid storage cavity 13 to release the reaction solution.
The bottom of the push plate 42 is provided with a groove 421, the top of the groove 421 is provided with a first absorbing member 422, and the top surface of the piston plate 43 is provided with a second absorbing member 431 which is attracted with the first absorbing member 422. The piston plate 43 and the push plate 42 are detachably connected, so that replacement is convenient.
The sealing member 3 comprises an annular pressing plate 31, a supporting plate 32 is arranged below the pressing plate 31, a sealing film 33 is arranged between the pressing plate 31 and the supporting plate 32, a top rupture disc 34 which is respectively arranged in the first liquid storage cavity 11, the second liquid storage cavity 12 and the third liquid storage cavity 13 is arranged on the sealing film 33, a cross-shaped cutting groove 35 which corresponds to the thimble 44 is arranged on the top rupture disc 34, and a liquid outlet hole 36 which corresponds to the top rupture disc 34 is arranged on the supporting plate 32. Sealing membrane 33 and clamp plate 31 all are equipped with sealing washer 38 between sealing membrane 33 and the backup pad 32, improve the leakproofness. When the cross-shaped cutting groove 35 on the top breaking sheet 34 is broken, liquid naturally flows out through the top breaking sheet 34 and the liquid outlet hole 36, the ejector pins 44 can not block the top breaking sheet 34, the ejector pins 44 are not required to be withdrawn, and the operation is convenient.
The sealing member 3 further comprises a plurality of U-shaped elastic clamping pieces 37, the lower ends of the elastic clamping pieces 37 are rotatably connected with the edges of the supporting plate 32, and the upper ends of the elastic clamping pieces 37 are buckled with the edges of the pressing plate 31. The diameter of the top flaps 34 is greater than the diameter of the ejector pins 44. The elastic card 37 is used for fixing the pressing plate 31, the sealing film 33 and the supporting plate 32, is detachable, is convenient for replacing the film, and the sealing piece 3 which is broken after the swab is used can be used for multiple times by replacing the sealing film 33, so that the waste is reduced, and the detection cost is reduced.
The flow tube 2 is in a conical shape with a large upper part and a small lower part, and the sampling tube 21 is detachably connected with the flow tube 2 through the clamping assembly 5.
The clamping assembly 5 comprises a fixing ring 51, a slot 52 for inserting the upper end of the sampling tube 21 is arranged at the bottom of the fixing ring 51, a plurality of fixing parts are arranged on the circumference of the inner wall of the slot 52, an embedded groove is arranged on one side of the fixing seat 53, and a clamping block 54 inserted into the embedded groove is arranged on the outer wall of the sampling tube 21. When the sampling tube 21 is disassembled, the clamping block 54 is rotated out of the embedded groove, so that the sampling tube 21 is separated from the fixed ring 51, sampling is facilitated, and after the sampling is finished, the sampling tube is mounted on the fixed seat 53, and the clamping block is rotationally clamped, so that the operation is convenient.
Claims (7)
1. A cold chain food outer package cleanliness detection method based on ATP is characterized in that: the method comprises the following steps:
firstly, taking a plurality of ATP swabs, wherein in every two ATP swabs, one ATP swab is filled with ATPase liquid and reaction liquid, and the other ATP swab is filled with ATPase liquid, selective bacterial culture liquid and reaction liquid; the total sampling area of the ATP swabs is the cold chain food outer packaging area, and the sampling mode is to wipe the surface of the cold chain food outer packaging in a wave rolling mode;
step two, placing a plurality of ATP swabs into the tube seat, and enabling a plurality of ejector pins on the tube seat to extend into the swabs respectively;
step three, parallelly sampling two sampling tubes filled with different reagents, and reinstalling the sampling tubes on a swab after sampling is completed;
pushing the push plate downwards to enable the ejector pin to eject a cavity for storing the ATPase liquid, wherein the ATPase liquid contacts with the sample to process unnecessary ATP;
step five, pushing the push plate continuously to enable the ejector pin to eject the cavity for storing the selective bacterial culture solution, enabling the selective bacterial culture solution to contact with the sample, and culturing and amplifying bacteria;
step six, pushing the push plate continuously to enable the thimble to push the cavity for storing the reaction liquid, and enabling the reaction liquid to contact with the sample for reaction;
step seven, the reacted ATP swab is placed into a detection bin of an ATP detection instrument for detection and comparison, and the cleanliness of the cold chain food outer package is known;
the swab comprises a liquid storage tube (1), a flow tube (2) is arranged below the liquid storage tube (1), a sealing piece (3) is arranged between the liquid storage tube (1) and the flow tube (2), a sampling tube (21) is arranged below the flow tube (2), and a reaction tube (22) is sleeved outside the sampling tube (21); a first liquid storage cavity (11), a second liquid storage cavity (12) and a third liquid storage cavity (13) which are isolated from each other are arranged in the liquid storage pipe (1); the first liquid storage cavity (11) is filled with ATPase liquid, the second liquid storage cavity (12) is filled with selective bacterial culture liquid, and the third liquid storage cavity (13) is filled with reaction liquid; the top of tube socket (4) is equipped with push pedal (42) of activity from top to bottom, and the bottom of push pedal (42) is equipped with a plurality of piston boards (43) that correspond with the swab one by one, and the bottom of piston board (43) is equipped with thimble (44) that stretch into respectively in first stock solution chamber (11), second stock solution chamber (12) and third stock solution chamber (13), and the length of three thimble (44) is different.
2. The ATP-based cold chain food outer package cleanliness detection method of claim 1, wherein: the sliding groove (45) is formed in the tube seat (4), the sliding rod (46) extending into the sliding groove (45) is arranged on the push plate (42), and three scale marks (47) for marking the sliding position are formed on one side of the sliding groove (45).
3. The ATP-based cold chain food outer package cleanliness detection method of claim 1, wherein: the bottom of push pedal (42) is equipped with recess (421), and the top of recess (421) is equipped with first adsorption equipment (422), and the top surface of piston board (43) is equipped with second adsorption equipment (431) that are attracted with first adsorption equipment (422).
4. The ATP-based cold chain food outer package cleanliness detection method of claim 1, wherein: the sealing piece (3) comprises an annular pressing plate (31), a supporting plate (32) is arranged below the pressing plate (31), a sealing film (33) is arranged between the pressing plate (31) and the supporting plate (32), top rupture discs (34) which are respectively arranged in the first liquid storage cavity (11), the second liquid storage cavity (12) and the third liquid storage cavity (13) are arranged on the sealing film (33), cross-shaped cutting grooves (35) corresponding to the ejector pins (44) are formed in the top rupture discs (34), and liquid outlet holes (36) corresponding to the top rupture discs (34) are formed in the supporting plate (32).
5. The ATP-based cold chain food outer package cleanliness detection method of claim 4, wherein: the sealing piece (3) further comprises a plurality of U-shaped elastic clamping pieces (37), the lower ends of the elastic clamping pieces (37) are rotationally connected with the edges of the supporting plate (32), and the upper ends of the elastic clamping pieces (37) are buckled with the edges of the pressing plate (31).
6. The ATP-based cold chain food outer package cleanliness detection method of claim 1, wherein: the flow tube (2) is in a conical shape with a large upper part and a small lower part, and the sampling tube (21) is detachably connected with the flow tube (2) through the clamping assembly (5).
7. The ATP-based cold chain food outer package cleanliness detection method of claim 6, wherein: the clamping assembly (5) comprises a fixing ring (51), a slot (52) for inserting the upper end of the sampling tube (21) is formed in the bottom of the fixing ring (51), a plurality of fixing parts are arranged on the circumference of the inner wall of the slot (52), an embedded groove is formed in one side of the fixing seat (53), and a clamping block (54) inserted into the embedded groove is arranged on the outer wall of the sampling tube (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110517302.6A CN113189075B (en) | 2021-05-12 | 2021-05-12 | ATP-based cold chain food outer package cleanliness detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110517302.6A CN113189075B (en) | 2021-05-12 | 2021-05-12 | ATP-based cold chain food outer package cleanliness detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113189075A CN113189075A (en) | 2021-07-30 |
| CN113189075B true CN113189075B (en) | 2023-12-15 |
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