CN114276928A - Virus transport culture medium suitable for nucleic acid detection - Google Patents
Virus transport culture medium suitable for nucleic acid detection Download PDFInfo
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- CN114276928A CN114276928A CN202111650043.0A CN202111650043A CN114276928A CN 114276928 A CN114276928 A CN 114276928A CN 202111650043 A CN202111650043 A CN 202111650043A CN 114276928 A CN114276928 A CN 114276928A
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Abstract
The invention discloses a virus transport culture medium suitable for nucleic acid detection, which belongs to the field of detection culture media and comprises a culture base body, wherein the culture base body comprises a sealing cover and a base, the sealing cover is sleeved on the top of the base, a plurality of jacks are formed in an annular array on the top of the base, a sampling swab is arranged on the top of the base through the jacks, the sampling swab is vertically and alternately arranged in the jacks, a closed ring is arranged on the sampling swab and close to the bottom, a reciprocating push rod is arranged in the center of the top of the base, a telescopic spring A is annularly wound on the side wall of the reciprocating push rod, an annular convex edge is arranged on the side wall of the base close to the top, the virus transport culture medium suitable for nucleic acid detection can be conveniently integrated at one position when the same object with a larger area is subjected to range detection, and can avoid some complicated procedures of sampling in the detection process, meanwhile, when a plurality of parts of the living body are detected, labeling and marking are convenient, and arrangement is convenient.
Description
Technical Field
The invention relates to the field of detection media, in particular to a virus conveying medium suitable for nucleic acid detection.
Background
The substance for nucleic acid detection is a nucleic acid of a virus. The nucleic acid detection is to find out whether the patient has the nucleic acid of the virus invaded from the outside in the respiratory tract specimen, blood or feces to determine whether the patient is infected by the virus, so that once the detection is positive, the patient can be proved to have the virus, because all organisms contain the nucleic acid, including deoxyribonucleic acid DNA and ribonucleic acid RNA, and the specific RNA sequence in the virus is one of the means for distinguishing the virus from other pathogens, after the virus appears, scientists find out the specific nucleic acid sequence in the virus by analyzing the whole genome sequence of the virus and comparing with the genome sequence of other species. In clinical laboratory tests, the possibility of a toxic infection of the article or living body is indicated if a specific nucleic acid sequence can be detected in the sample.
In virus detection, sampling becomes a non-negligible step, after sampling is completed, the culture medium needs to be transported to a detection center for detection, a transport culture medium is one of the most critical sampling tools, the transport culture medium is a culture medium for protecting and maintaining the activity of microorganisms after sampling and before laboratory sample treatment, substances for proliferating the microorganisms are generally not allowed to be contained in the transport culture medium, but the culture medium can protect strains and ensure that the strains do not deteriorate, in the prior art, the local area sampling detection is complicated in the process of sampling detection of the transport culture medium, the culture medium needs to be taken out one by one to be wiped for sampling, the collection is inconvenient for marking sampling positions, in the process of living body detection, multiple-position detection is needed, and the number of individual detection is large and is not beneficial to integration.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide a virus conveying culture medium suitable for nucleic acid detection, which can realize the range detection of the same object with a larger area, is convenient to integrate at one position, can avoid some complicated procedures of sampling in the detection process, and is convenient for labeling and arranging when detecting a plurality of parts of a living body.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
The utility model provides a virus transport culture medium suitable for nucleic acid detects, includes the culture base body, the culture base body is including sealed lid and basement, sealed lid cup joints the top at the basement, a plurality of jacks have been seted up to the top annular array of basement, and the top of basement is provided with the sampling swab through the jack, the vertical interlude of sampling swab sets up in the inside of jack, and on the sampling swab and be close to the bottom and be provided with the closed ring, the top central authorities of basement are provided with reciprocal push rod, the annular winding of the lateral wall of reciprocal push rod has expanding spring A, expanding spring A's one end is fixed at the top of basement, and the other end is fixed on reciprocal push rod, and the lateral wall of basement is close to the top and is provided with annular bead.
Furthermore, a plurality of fixing holes are formed in the top wall inside the sealing cover, and the fixing holes are arranged corresponding to the insertion holes.
Furthermore, the inner wall of the bottom of the sealing cover is provided with a concave ring, the annular convex edge and the concave ring are correspondingly arranged and correspondingly embedded in a clamping manner, and a sealing rubber gasket is arranged below the concave ring and at the bottom of the sealing cover.
Furthermore, the bottom of the base is provided with a liquid taking hole corresponding to the insertion hole, and the insertion hole and the closed ring can be correspondingly embedded.
Furthermore, the top of the base is provided with a traveling channel corresponding to the reciprocating push rod, a culture solution bin is arranged inside the base, the traveling channel is communicated with the culture solution bin, the bottom end of the reciprocating push rod extends to the inside of the culture solution bin through the traveling channel, and a piston is installed at the bottom end of the reciprocating push rod.
Furthermore, a liquid containing bin is arranged in the base corresponding to the insertion hole, the liquid containing bin is communicated with the insertion hole, a flow guide channel is arranged between the liquid containing bin and the culture liquid bin, a valve shell is arranged at the bottom of the culture liquid bin corresponding to the flow guide channel, a pressure hole is formed in the bottom of the valve shell, a pressure-controlled ball valve is arranged in the valve shell corresponding to the pressure, and a telescopic spring B is arranged on one side, corresponding to the pressure hole, of the ball valve.
Furthermore, the flow guide channel is obliquely arranged between the liquid containing bin and the culture liquid bin, and the inclination angle of one end of the culture liquid bin is larger than that of one end of the flow guide channel.
Furthermore, the bottom center of the liquid containing bin is provided with a bulge, and the liquid taking hole is arranged corresponding to the bulge.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) this scheme, through set up a plurality of jacks along the center at the top of basement, sampling swab in the jack facilitates the use, examines time measuring in the sampling, carries out scope nature to the great same object of area and examines time measuring, is convenient for concentrate the integration in one, pastes the mark, does benefit to the arrangement, can avoid some loaded down with trivial details procedures of testing process sampling, sets up the closed loop in sampling swab's bottom, and sampling swab corresponds the jack and inserts, and closed loop shutoff jack prevents to remove the inside weeping of in-process basement.
(2) This scheme, correspond annular bead through the bottom inner wall at sealed lid and set up the concave ring to annular bead and the corresponding inlay card of concave ring, the inside sampling swab of protection that can be better, and set up sealed cushion in the bottom of sealed lid, make the inner wall of sealed lid and the close laminating of basement lateral wall, correspond the jack with the bottom of basement and seted up and get the liquid hole, when inside liquid is taken out to needs, the sampling needle with get the liquid hole and align, reach the effect of punctuation, the puncture sample of being convenient for.
(3) In the scheme, the reciprocating push rod is wound and installed on the reciprocating push rod through the reciprocating push rod at the center of the top of the base, so that the reciprocating push rod can rebound back and forth, a piston is arranged at the bottom of the reciprocating push rod, when the reciprocating push rod is pushed to move downwards, the piston applies pressure to the interior of the culture solution bin to move the culture solution in the culture solution bin to the bottom, the pressure intensity at the bottom of the culture solution bin is overlarge, the ball valve is jacked up through the bottom pressure hole of the valve shell, so that the culture solution enters the liquid containing bin through the flow guide channel, the cotton swab at the bottom of the sampling swab is soaked in the liquid, the activity of the collected sample is ensured, when the internal pressure of the culture solution cabin is removed, the telescopic spring B applies pressure to the ball valve to block the bottom pressure hole of the valve shell so as to avoid liquid backflow and leakage in the liquid containing cabin, and the flow guide channel is obliquely arranged between the liquid containing bin and the culture liquid bin, so that the risk of liquid backflow is further avoided.
Drawings
FIG. 1 is a schematic view of the seal cap and base construction of the present invention;
FIG. 2 is a schematic view of the bottom structure of the substrate of the present invention;
FIG. 3 is a schematic cross-sectional view of the sealing cover and the base of the present invention;
FIG. 4 is a schematic view of the internal cross-sectional structure of the substrate of the present invention;
FIG. 5 is an enlarged detail view A of FIG. 3 according to the present invention;
FIG. 6 is an enlarged detail view of B in FIG. 4 according to the present invention;
FIG. 7 is a schematic view of the overall structure of the present invention;
FIG. 8 is a schematic structural view of a culture medium body in example 2 of the present invention;
FIG. 9 is a diagram of the structure of the softer layer according to the present invention.
The reference numbers in the figures illustrate:
1. a culture medium body; 2. a sealing cover; 3. a substrate; 4. a jack; 5. sampling a swab; 6. a closed ring; 7. a reciprocating push rod; 8. a telescopic spring A; 9. an annular rib; 10. a fixing hole; 11. a concave ring; 12. sealing the rubber gasket; 13. a liquid extraction hole; 14. a travel path; 15. a culture solution cabin; 16. a piston; 17. a liquid containing bin; 18. a flow guide channel; 19. a valve housing; 20. a ball valve; 21. a telescopic spring B; 22. a protrusion; 23. a connecting channel; 24. a soft layer; 25. a sealing layer; 26. A hole; 27. a flow deflector; 28. a liquid storage sac; 29. connecting sheets; 30. and (5) pricking sheets.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.
Example 1:
referring to fig. 1-7, a virus transport medium suitable for nucleic acid detection comprises a culture medium body 1, the culture medium body 1 comprises a sealing cover 2 and a base 3, the sealing cover 2 is sleeved on the top of the base 3, a plurality of insertion holes 4 are formed in an annular array on the top of the base 3, sampling swabs 5 are arranged on the top of the base 3 through the insertion holes 4, the sampling swabs 5 are vertically inserted into the insertion holes 4, a closed ring 6 is arranged on the sampling swabs 5 and close to the bottom, a reciprocating push rod 7 is arranged in the center of the top of the base 3, a telescopic spring A8 is annularly wound on the side wall of the reciprocating push rod 7, one end of a telescopic spring A8 is fixed on the top of the base 3, the other end of the telescopic spring a is fixed on the reciprocating push rod 7, an annular rib 9 is arranged on the side wall of the base 3 close to the top, a plurality of insertion holes 4 are formed along the center, the top of the base 3, and the sampling swabs 5 in the insertion holes 4 are convenient to use, during the sampling inspection, when carrying out scope nature inspection to the great same object of area, be convenient for concentrate on the integration and in one, be convenient for paste the mark, do benefit to the arrangement, can avoid some loaded down with trivial details procedures that the testing process sampled, set up closed ring 6 in the bottom of sampling swab 5, sampling swab 5 corresponds jack 4 and inserts, and 6 shutoff jacks 4 of closed ring prevent to remove the inside weeping of in-process basement 3.
Referring to fig. 1-2, a plurality of fixing holes 10 are formed on the top wall inside the sealing cover 2, the fixing holes 10 are disposed corresponding to the insertion holes 4, the fixing holes 10 are formed on the top wall inside the sealing cover 2 corresponding to the insertion holes 4, and after sampling, the sealing cover 2 is sleeved on the substrate 3 for fixing, so that the sampling swab 5 can be stabilized inside the fixing holes 10.
Referring to fig. 1-2, the inner wall of the bottom of the sealing cover 2 is provided with a concave ring 11, the annular protruding rib 9 is disposed corresponding to the concave ring 11 and is correspondingly embedded, the sealing rubber pad 12 is disposed below the concave ring 11 and at the bottom of the sealing cover 2, the concave ring 11 is disposed corresponding to the annular protruding rib 9 at the inner wall of the bottom of the sealing cover 2, and the annular protruding rib 9 and the concave ring 11 are correspondingly embedded, so as to better protect the sampling swab 5 inside, and the sealing rubber pad 12 is disposed at the bottom of the sealing cover 2, so that the inner wall of the sealing cover 2 is closely attached to the sidewall of the base 3.
Referring to fig. 2-3, the bottom of the base 3 is provided with a liquid-extracting hole 13 corresponding to the insertion hole 4, and the insertion hole 4 and the closed ring 6 can be correspondingly embedded.
Referring to fig. 4 or 6, a traveling channel 14 is provided at the top of the base 3 corresponding to the reciprocating push rod 7, a culture solution chamber 15 is provided inside the base 3, the traveling channel 14 is communicated with the culture solution chamber 15, the bottom end of the reciprocating push rod 7 extends to the inside of the culture solution chamber 15 through the traveling channel 14, a piston 16 is installed at the bottom end of the reciprocating push rod 7, a liquid containing chamber 17 is provided at the inside of the base 3 corresponding to the insertion hole 4, the liquid containing chamber 17 is communicated with the insertion hole 4, a flow guide channel 18 is provided between the liquid containing chamber 17 and the culture solution chamber 15, a valve housing 19 is provided at the bottom of the culture solution chamber 15 corresponding to the flow guide channel 18, a pressure hole is opened at the bottom of the valve housing 19, a pressure-controlled ball valve 20 is correspondingly provided inside the valve housing 19, a telescopic spring B21 is provided at one side of the ball valve 20 corresponding to the pressure hole, a telescopic spring a8 is wound on the reciprocating push rod 7 at the top center of the base 3, make reciprocal push rod 7 can kick-back, set up piston 16 in the bottom of reciprocal push rod 7, when promoting reciprocal push rod 7 downstream, piston 16 exerts pressure to the inside in culture solution storehouse 15, with the inside culture solution of culture solution storehouse 15 to the bottom motion, the bottom pressure in culture solution storehouse 15 is too big, presses the hole through the bottom of valve shell 19, jack-up ball valve 20, makes the culture solution pass through the inside of water conservancy diversion passageway 18 entering flourishing liquid storehouse 17, makes sampling swab 5 bottom cotton swab soak in liquid, guarantees the activity of gathering the sample.
Referring to fig. 4, the flow guiding channel 18 is obliquely disposed between the liquid containing chamber 17 and the culture solution chamber 15, and the inclination angle of one end of the culture solution chamber 15 is greater than the inclination angle of one end of the flow guiding channel 18, when the internal pressure of the culture solution chamber 15 is removed, the expansion spring B21 applies pressure to the ball valve 20 to block the bottom pressure hole of the valve housing 19, so as to prevent the liquid inside the liquid containing chamber 17 from flowing back and leaking, and the flow guiding channel 18 is obliquely disposed between the liquid containing chamber 17 and the culture solution chamber 15, so as to further avoid the risk of liquid backflow.
Referring to fig. 4, a protrusion 22 is disposed at the center of the bottom of the liquid containing bin 17, the liquid taking hole 13 is disposed corresponding to the protrusion 22, the liquid taking hole 13 is disposed at the bottom of the base 3 corresponding to the insertion hole 4, when the internal liquid needs to be taken out, the sampling needle is aligned with the liquid taking hole 13 to achieve the function of marking, so as to facilitate puncture sampling, the protrusion 22 can be made of soft rubber material or other soft materials, and a certain sealing effect can be maintained after liquid taking.
Example 2:
referring to fig. 7, a connecting channel 23 is disposed between two adjacent liquid storage bins 17, and the connecting channel 23 mixes a plurality of sampling swab samples, so that the whole set of samples can be detected simultaneously, the batch detection efficiency is higher, and the method is suitable for large-scale detection.
Example 3:
referring to fig. 4 and 7-9, based on the embodiment 1-2, a soft layer 24 is bonded to the bottom of a substrate 3, a sealing layer 25 is disposed on the other side of the soft layer 24, holes 26 are uniformly distributed on the inner side of the soft layer 24, a baffle 27 is disposed between two adjacent holes 26, the baffle 27 extends into the holes 26, a liquid bag is disposed on the inner side of the hole 26, the liquid bag is composed of a plurality of liquid storage sub-bags 28, the liquid storage sub-bags 28 are tightly attached to the inner walls of the holes 26 and are in contact with the baffle 27, a disinfectant is stored in the liquid storage sub-bags 28, a connecting sheet 29 is disposed at the bottom of the liquid bags, a spike sheet 30 is disposed at the bottom of the connecting sheet 29, a gap is left between the spike sheet 30 and the sealing layer 25, and the spike sheet 30 can puncture the sealing layer 25 when being subjected to a large external force and cannot be affected by a small external force, specifically, the soft layer 24 can be made of an airtight soft material with a porous structure, sponge can be adopted, glue layers are coated on the outer side surfaces of the sponge for sealing, when detection is needed, the culture medium base body 1 is pressed, at the moment, the disinfectant in the liquid storage sub-bag 28 overflows due to extrusion force, the bottom of the base 3 can be disinfected, so that impurities are prevented from polluting a detection sample when a needle head takes liquid, then the soft layer 24 and the sealing layer 25 are uncovered, and the base 3 can be further wiped and disinfected; furthermore, the sealing layer 25 can seal the bottom of the substrate 3 to keep the bottom of the substrate 3 clean, so that before the culture medium body 1 is used, whether the culture medium body 1 is polluted or not can be determined by observing whether holes exist in the sealing layer 25 or whether disinfectant overflows, and similarly, the identification effect can be improved by using disinfectant liquid with color inside the liquid storage sub-bag 28.
When in use: the top of the base 3 is provided with a plurality of jacks 4 along the center, the sampling swabs 5 in the jacks 4 are convenient to use, during sampling detection, when the same object with a larger area is subjected to range detection, the sampling swabs are conveniently integrated at one position, labeling marks are marked, arrangement is facilitated, some complicated sampling procedures in the detection process can be avoided, the bottom of each sampling swab 5 is provided with the closed ring 6, the sampling swabs 5 are inserted corresponding to the jacks 4, the closed rings 6 block the jacks 4 to prevent leakage inside the base 3 in the moving process, the top wall inside the sealing cover 2 is provided with the fixing holes 10 corresponding to the jacks 4, after sampling is completed, the sealing cover 2 is sleeved on the base 3 to be fixed, the sampling swabs 5 can be stabilized inside the fixing holes 10, the inner wall of the bottom of the sealing cover 2 is provided with the concave rings 11 corresponding to the annular convex ribs 9, and the annular convex ribs 9 are correspondingly clamped and embedded with the concave rings 11, so that the sampling swabs 5 inside can be well protected, and a sealing rubber pad 12 is arranged at the bottom of the sealing cover 2, so that the inner wall of the sealing cover 2 is closely attached to the side wall of the substrate 3, a reciprocating push rod 7 is arranged at the center of the top of the substrate 3, a telescopic spring A8 is wound on the reciprocating push rod 7, so that the reciprocating push rod 7 can rebound back in a reciprocating manner, a piston 16 is arranged at the bottom of the reciprocating push rod 7, when the reciprocating push rod 7 is pushed to move downwards, the piston 16 applies pressure to the inside of a culture solution bin 15, culture solution in the culture solution bin 15 moves towards the bottom, the bottom pressure of the culture solution bin 15 is overlarge, a ball valve 20 is jacked up through a bottom pressure hole of a valve shell 19, so that the culture solution enters the inside of a liquid containing bin 17 through a flow guide channel 18, a cotton swab at the bottom of a sampling swab 5 is soaked in the liquid, the activity of a sample is ensured, when the internal pressure of the culture solution bin 15 is removed, a telescopic spring B21 applies pressure to the ball valve 20, so as to block the bottom pressure hole of the valve shell 19, avoid flourishing liquid storehouse 17 inside liquid backward flow weeping to the slope of water conservancy diversion passageway 18 sets up between flourishing liquid storehouse 17 and culture solution storehouse 15, further avoids the risk of liquid adverse current, corresponds jack 4 with the bottom of basement 3 and has seted up and get liquid hole 13, when needs take out inside liquid, the sampling needle aligns with getting liquid hole 13, reaches the effect of punctuation, is convenient for puncture the sample.
The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.
Claims (8)
1. A virus transport medium suitable for nucleic acid detection comprises a medium body (1), wherein the medium body (1) comprises a sealing cover (2) and a substrate (3), the sealing cover (2) is sleeved on the top of the substrate (3), and the virus transport medium is characterized in that: a plurality of jacks (4) have been seted up to the top annular array of base (3), and the top of base (3) is provided with sampling swab (5) through jack (4), sampling swab (5) vertical interlude sets up in the inside of jack (4), and sampling swab (5) are gone up and are provided with closed ring (6) near the bottom, and the top central authorities of base (3) are provided with reciprocal push rod (7), the annular winding of the lateral wall of reciprocal push rod (7) has expanding spring A (8), the top in base (3) is fixed to the one end of expanding spring A (8), and the other end is fixed on reciprocal push rod (7), and the lateral wall of base (3) is close to the top and is provided with annular bead (9).
2. A viral delivery medium suitable for use in nucleic acid detection according to claim 1, wherein: a plurality of fixing holes (10) are formed in the top wall inside the sealing cover (2), and the fixing holes (10) are arranged corresponding to the insertion holes (4).
3. A viral delivery medium suitable for use in nucleic acid detection according to claim 1, wherein: concave ring (11) have been seted up to the bottom inner wall of sealed lid (2), annular bead (9) correspond with concave ring (11) and set up and corresponding inlay card, the below of concave ring (11) and the bottom that is located sealed lid (2) are provided with sealed cushion (12).
4. A viral delivery medium suitable for use in nucleic acid detection according to claim 1, wherein: the bottom of the base (3) is provided with a liquid taking hole (13) corresponding to the insertion hole (4), and the insertion hole (4) and the closed ring (6) can be correspondingly embedded.
5. A viral delivery medium suitable for use in nucleic acid detection according to claim 1, wherein: the top of basement (3) corresponds reciprocal push rod (7) and is equipped with marching passageway (14), and the inside of basement (3) is provided with culture solution storehouse (15), marching passageway (14) and culture solution storehouse (15) intercommunication, the bottom of reciprocal push rod (7) extends to the inside of culture solution storehouse (15) through marching passageway (14), piston (16) are installed to the bottom of reciprocal push rod (7).
6. A viral delivery medium suitable for use in nucleic acid detection according to claim 1, wherein: the liquid storage device is characterized in that a liquid storage bin (17) is arranged in the base (3) corresponding to the insertion hole (4), the liquid storage bin (17) is communicated with the insertion hole (4), a flow guide channel (18) is arranged between the liquid storage bin (17) and the culture solution bin (15), a valve shell (19) is arranged at the bottom of the culture solution bin (15) corresponding to the flow guide channel (18), a pressure hole is formed in the bottom of the valve shell (19), a ball valve (20) is arranged in the valve shell (19) corresponding to the pressure control, and a telescopic spring B (21) is arranged on one side, corresponding to the pressure hole, of the ball valve (20).
7. A viral delivery medium suitable for use in nucleic acid detection according to claim 6, wherein: the flow guide channel (18) is obliquely arranged between the liquid containing bin (17) and the culture solution bin (15), and the inclination angle of one end of the culture solution bin (15) is larger than that of one end of the flow guide channel (18).
8. A viral delivery medium suitable for use in nucleic acid detection according to claim 6, wherein: the bottom center of the liquid containing bin (17) is provided with a bulge (22), and the liquid taking hole (13) is arranged corresponding to the bulge (22).
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| CN202111650043.0A CN114276928A (en) | 2021-12-30 | 2021-12-30 | Virus transport culture medium suitable for nucleic acid detection |
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| CN202111650043.0A CN114276928A (en) | 2021-12-30 | 2021-12-30 | Virus transport culture medium suitable for nucleic acid detection |
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