CN111504207A - Blood cell centrifugal layering detection system and method - Google Patents
Blood cell centrifugal layering detection system and method Download PDFInfo
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- CN111504207A CN111504207A CN202010515928.9A CN202010515928A CN111504207A CN 111504207 A CN111504207 A CN 111504207A CN 202010515928 A CN202010515928 A CN 202010515928A CN 111504207 A CN111504207 A CN 111504207A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
- G01B11/06—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness for measuring thickness ; e.g. of sheet material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
- G01B11/028—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness by measuring lateral position of a boundary of the object
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/10—Segmentation; Edge detection
- G06T7/13—Edge detection
Abstract
The invention discloses a blood cell centrifugal layering detection system and a blood cell centrifugal layering detection method, which solve the technical problem that when a reflection type photoelectric sensor is adopted to measure a blood centrifugal layering interface in the prior art, the measurement precision is not ideal due to the interference of an external light source, the attenuation influence of the light power of an emitter and the influence of consumable processing precision. The detection system comprises a centrifugal cup, a light source, a reflector, a visual identification sensor and an image data processing module. The method mainly utilizes the reflector to project the light source to the shoulder part of the centrifugal cup, and simultaneously reflects the reflected light to the video camera sensor for imaging through the reflector so as to accurately obtain the thickness of each layer of the blood cup type centrifugal layering and the position information of the interface. The invention can solve the influence of ambient light interference, light source emitter attenuation, installation position precision, centrifugal cup processing precision and the like on the thickness measurement of each layer of the blood cup type centrifugal layering and the interface position measurement, thereby greatly improving the detection precision of the layering thickness and the interface position.
Description
Technical Field
The invention relates to the technical field of whole blood separation, in particular to a blood cell centrifugal layering detection system and a blood cell centrifugal layering detection method.
Background
In the whole blood cup type centrifugal separation process, the blood centrifugal layering interface needs to be accurately measured so as to accurately control and collect single components (blood plasma, blood platelets, white blood cells, red blood cells and the like), and the measurement accuracy directly determines the collection quality of the single components.
The traditional detection mode is that a reflection type photoelectric sensor is adopted to measure a blood centrifugal layering interface, and the reflection type photoelectric sensor is easily influenced by an external light source in the measurement process, so that the requirement on the installation precision of the reflection type photoelectric sensor is very high, meanwhile, the reflection type photoelectric sensor is greatly influenced by the optical power attenuation of an emitter, the precision is not ideal when the measurement is carried out, the processing precision of consumables has a great influence on the detection result, and under the comprehensive influence of the factors, the reflection type photoelectric sensor is difficult to meet the requirements on high-precision control and use convenience.
Therefore, the blood cell centrifugal layering detection system and method provided by the invention project a light source to the shoulder of the centrifugal cup by using the reflector, and reflect reflected light to the video camera sensor for imaging by using the reflector, so as to accurately acquire thickness and interface position information of each layer of the blood cup type centrifugal layering, and the technical problem to be solved by technical personnel in the technical field is urgently solved.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a blood cell centrifugal layering detection system and a method. The technical problem that when a reflection-type photoelectric sensor is used for measuring a blood centrifugal layering interface in the prior art, the measurement precision is not ideal enough due to the interference of an external light source, the attenuation influence of the luminous power of an emitter and the influence of the processing precision of consumable materials is solved.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a centrifugal stratified blood cell testing system comprising:
a centrifuge cup for centrifuging and layering blood;
a light source for emitting a light beam;
a mirror;
the vision identification sensor and the image data processing module are in signal connection with each other;
the image data processing module receives imaging data information transmitted by the visual recognition sensor and then calculates and processes the accurate positions and thicknesses of a plasma layer, a leucocyte layer and a erythrocyte layer in the centrifugal cup.
Further, the device also comprises an image recognition module, and the visual recognition sensor is connected with the image data processing module through the image recognition module.
And the liquid crystal display screen is in signal connection with the image data processing module and is used for displaying the accurate positions and thickness information of a plasma layer, a leucocyte layer and a red blood cell layer in the centrifugal cup in real time.
Further, the leukocyte layer includes a platelet layer, a leukocyte layer, and a stem cell layer.
Further, the reflector has a plurality of pieces.
Furthermore, a plurality of reflectors jointly reflect light beams emitted by the light source to the layered interface of the centrifugal cup.
Furthermore, a plurality of reflectors reflect the light beams reflected by the centrifugal cup layered interface to the visual identification sensor together.
A detection method of a blood cell centrifugal layering detection system comprises the steps that a light source emits light beams and projects the light beams to a reflector, the reflector reflects the light beams to a centrifugal cup layering interface, the centrifugal cup layering interface reflects the light beams to the reflector again, the reflector reflects the light beams projected by the centrifugal cup layering interface to a visual identification sensor, the visual identification sensor receives the light beams projected by the reflector and images the light beams and transmits imaging information to an image data processing module, and the image data processing module accurately calculates and processes accurate positions and thicknesses of a plasma layer, a leucocyte layer and a red blood cell layer in a centrifugal cup according to the received information.
Compared with the prior art, the invention has the following beneficial effects:
the blood cup type centrifugal layering device is simple in structure, scientific and reasonable in design and convenient to use, a light source is projected to the shoulder of the centrifugal cup by the aid of the reflector, and meanwhile reflected light is reflected to the video camera sensor through the reflector to be imaged, so that thickness of each layer of the blood cup type centrifugal layering and interface position information are accurately acquired.
The invention can solve the influence of ambient light interference, light source emitter attenuation, installation position precision, centrifugal cup processing precision and the like on the thickness measurement of each layer of the blood cup type centrifugal layering and the interface position measurement, thereby greatly improving the detection precision of the layering thickness and the interface position.
Drawings
FIG. 1 is a schematic view of the structure of the present invention.
Fig. 2 is a connection block diagram of each electrical device of the present invention.
Wherein, the names corresponding to the reference numbers are:
the system comprises a 1-reflector, a 2-light source, a 3-visual identification sensor, a 4-centrifugal cup, a 5-image data processing module, a 6-image identification module, a 7-liquid crystal display screen, an 8-plasma layer, a 9-leucocyte layer and a 10-erythrocyte layer.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation or be constructed and operated in a particular orientation, and thus, it should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; of course, mechanical connection and electrical connection are also possible; alternatively, they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
As shown in fig. 1 and 2, the present invention provides a blood cell centrifugal stratification detecting system, which comprises: the blood treatment device comprises a centrifugal cup 4 for carrying out centrifugal layering on blood, a light source 2 for emitting light beams, a reflector 1, a visual identification sensor 3 and an image data processing module 5 which are in signal connection with each other, an image identification module 6 and a liquid crystal display screen 7; the device comprises a light source 2, a visual identification sensor 3, an image data processing module 5, a blood plasma layer, a leucocyte layer and a stem cell layer, wherein a light beam emitted by the light source 2 sequentially passes through a layered interface of a reflector 1 and a centrifugal cup 4 and the reflector 1 and then is projected to the visual identification sensor 3 for accurate imaging, the image data processing module 5 receives imaging data information transmitted by the visual identification sensor 3 and then calculates and processes accurate positions and thicknesses of the blood plasma layer, the leucocyte layer and the stem cell layer in the centrifugal cup 4, and the leucocyte layer comprises a leucocyte layer, a leucocyte. The vision recognition sensor 3 is connected with the image data processing module 5 through the image recognition module 6. The liquid crystal display screen 7 is in signal connection with the image data processing module 5 and is used for displaying the accurate positions and thickness information of a plasma layer, a leucocyte layer and a red blood cell layer in the centrifugal cup 4 in real time.
The reflecting mirror 1 is provided with a plurality of reflecting mirrors 1, the reflecting mirrors 1 jointly reflect light beams emitted by the light source 2 to the layered interface of the centrifugal cup 4, and the reflecting mirrors 1 jointly reflect light beams emitted by the layered interface of the centrifugal cup 4 to the visual identification sensor 3.
The blood cup type centrifugal layering device is simple in structure, scientific and reasonable in design and convenient to use, a light source is projected to the shoulder of the centrifugal cup by the aid of the reflector, and meanwhile reflected light is reflected to the video camera sensor through the reflector to be imaged, so that thickness of each layer of the blood cup type centrifugal layering and interface position information are accurately acquired.
The invention provides a detection method of a blood cell centrifugal layering detection system, wherein a light source emits a light beam and projects the light beam to a reflector, the reflector reflects the light beam to a centrifugal cup layering interface, the centrifugal cup layering interface reflects the light beam to the reflector, the reflector reflects the light beam projected by the centrifugal cup layering interface to a visual identification sensor, the visual identification sensor receives the light beam projected by the reflector and images and transmits imaging information to an image data processing module, and the image data processing module accurately calculates and processes the accurate positions and thicknesses of a plasma layer 8, a tunica albuginea layer 9 and a red blood cell layer 10 in a centrifugal cup according to the received information.
The invention can solve the influence of ambient light interference, light source emitter attenuation, installation position precision, centrifugal cup processing precision and the like on the thickness measurement of each layer of the blood cup type centrifugal layering and the interface position measurement, thereby greatly improving the detection precision of the layering thickness and the interface position.
The invention projects the light beam emitted by the light source to the shoulder part (layered interface of the centrifugal cup) of the centrifugal cup through the reflector, reflects the reflected light to the video camera sensor for imaging through the plurality of reflectors, and calculates the reflected image for the air layer, the plasma layer, the white membrane layer (platelet, leukocyte, stem cell layer) and the red blood cell layer through the Canny edge detection operator edge detection algorithm, so that the thickness of each layer, the position of the interface and the like of the blood cup type centrifugal layered layer can be accurately identified.
The invention can ensure the correctness of the measuring result within a certain installation error range, allows the power of the light source to be changed, has high blood layering detection precision and increases the allowable error range of the processing precision of consumable materials.
The focal length of a visual identification camera sensor in the market is generally 50mm to 90mm, and the visual identification camera sensor cannot be directly installed in a centrifugal separation device for blood cup type centrifugal layering thickness and interface position imaging measurement due to the fact that the focal length is too short.
When the invention is used, firstly, a reflector group, a vision sensor and a light source are arranged according to the direction shown in figure 1, and a sample centrifugal cup is arranged; then, adjusting the visual sensor and the reflector module to ensure that the layered interface to be measured is in the visual field range of the visual sensor; then, adjusting the position and the angle of a light source to ensure that the illumination of the layered interface part to be measured is basically uniform; and finally, adjusting the focal length of the visual sensor to enable the focal length of the visual sensor to be within an ideal imaging range, transmitting imaging information to the image data processing module through the image recognition module by the visual sensor, and displaying the layering thickness information and the interface position information which are subjected to operation processing in real time through the liquid crystal display screen by the image data processing module.
Finally, it should be noted that: the above embodiments are only preferred embodiments of the present invention to illustrate the technical solutions of the present invention, but not to limit the technical solutions, and certainly not to limit the patent scope of the present invention; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention; that is, the technical problems to be solved by the present invention, which are not substantially changed or supplemented by the spirit and the concept of the main body of the present invention, are still consistent with the present invention and shall be included in the scope of the present invention; in addition, the technical scheme of the invention is directly or indirectly applied to other related technical fields, and the technical scheme is included in the patent protection scope of the invention.
Claims (8)
1. A centrifugal stratified blood cell testing system, comprising:
a centrifuge cup (4) for centrifuging and layering blood;
a light source (2) for emitting a light beam;
a mirror (1);
the vision recognition sensor (3) and the image data processing module (5) are in signal connection with each other;
the device is characterized in that a light beam emitted by the light source (2) sequentially passes through the reflector (1), the layered interface of the centrifugal cup (4) and the reflector (1) and then is projected to the visual recognition sensor (3) for accurate imaging, and the image data processing module (5) receives imaging data information transmitted by the visual recognition sensor (3) and then calculates and processes accurate positions and thicknesses of a plasma layer, a leucocyte layer and a erythrocyte layer in the centrifugal cup (4).
2. The centrifugal stratification detection system for blood cells according to claim 1, further comprising an image recognition module (6), wherein the visual recognition sensor (3) is connected with the image data processing module (5) through the image recognition module (6).
3. The centrifugal layered detection system for blood cells according to claim 1, further comprising a liquid crystal display (7) in signal connection with the image data processing module (5) for displaying the accurate position and thickness information of the plasma layer, the leucocyte layer and the erythrocyte layer in the centrifuge cup (4) in real time.
4. The centrifugal stratified blood cell testing system as recited in claim 1, wherein said buffy coat layer comprises a platelet layer, a buffy coat layer and a stem cell layer.
5. The centrifugal stratified blood cell detection system as claimed in claim 1, wherein the reflecting mirror (1) has a plurality of pieces.
6. The centrifugal layering detection system for blood cells as claimed in claim 5, wherein the plurality of reflectors (1) are used together to reflect the light beam emitted by the light source (2) to the layering interface of the centrifugal cup (4).
7. The blood cell centrifugal layering detection system according to claim 6, wherein a plurality of reflectors (1) are used together to reflect the light beam reflected by the layering interface of the centrifugal cup (4) to the visual identification sensor (3).
8. The detecting method of the centrifugal layered detecting system for blood cells of any one of claims 1-7, wherein the light source emits a light beam and projects the light beam to the reflector, the reflector reflects the light beam to the layered interface of the centrifugal cup, the layered interface of the centrifugal cup reflects the light beam to the reflector, the reflector reflects the light beam projected by the layered interface of the centrifugal cup to the visual recognition sensor, the visual recognition sensor receives the light beam projected by the reflector and images the light beam and transmits the imaging information to the image data processing module, and the image data processing module processes the accurate position and thickness of the plasma layer, the leukocyte layer and the erythrocyte layer in the centrifugal cup according to the received information.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010515928.9A CN111504207A (en) | 2020-06-09 | 2020-06-09 | Blood cell centrifugal layering detection system and method |
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| CN202010515928.9A CN111504207A (en) | 2020-06-09 | 2020-06-09 | Blood cell centrifugal layering detection system and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116694829A (en) * | 2023-08-04 | 2023-09-05 | 北京邦宁智科生物技术有限公司 | Cell centrifugation control method and cell separation equipment based on multi-scale visual perception |
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