CN111494466A - A topical composition containing Cannabis sativa extract and its application - Google Patents
A topical composition containing Cannabis sativa extract and its application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses an external composition containing a hemp extract, which is a preparation prepared by taking the hemp extract as an active ingredient and adding auxiliary materials acceptable in cosmetics, wherein the hemp extract is an extract prepared by adding an ethanol water solution into hemp leaves, hemp flowers and hemp stems in a mass ratio of 5-7: 2-4: 1.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicines, and particularly relates to an external composition containing an industrial hemp extract and application thereof.
Background
Sensitive skin is a skin state with increased local reactivity, and the skin of a specific part of a patient is sensitive to slight conventional external stimulation, cannot tolerate common skin care products and cleaning products, or has reduced tolerance to the skin after long-term use of the products, has symptoms of different degrees of pruritus, burning, tightness, dryness, stabbing pain, swelling and the like, and serious patients can have erythema, desquamation, papules, pustules and the like. The three major causes of sensitive skin are excessive skin care, glucocorticoid abuse and genetic susceptibility genes. At present, no complete theory is available for the pathogenesis of sensitive skin, and most dermatologists and scientific researchers believe that sensitive skin is mainly caused by impaired skin barrier, decreased water content of skin, incomplete stratum corneum structure, subcutaneous vasodilation or inflammatory factors, and the like.
Industrial hemp (Cannabis sativa L) has important agricultural and medical values, and with the intensive research on various functional components (cannabinol, cannabinone, cannabinolic acid, unsaturated fatty acid, etc.) of hemp extract, the application of hemp extract in medicine, daily use, textile and cosmetics is receiving more and more extensive attention.
In the prior art, a report of applying a cannabis extract to skin care is provided, for example, patent CN 107802530 a discloses a cannabis extract-containing composition, which has the effects of moisturizing, resisting inflammation and resisting aging by controlling the content of cannabis phenols in the cannabis extract, but in terms of effect experiments, the composition can be only used for daily care of ordinary skin, and cannot play a role in caring and caring sensitive skin with a relatively thin stratum corneum, and meanwhile, cannabinol contains active ingredients such as tetrahydrocannabinol which has a strong effect on the central nervous system, so that mental dependence can be generated by improper dosage of cannabis phenol, and potential harm of cannabis phenol cannot be ignored.
At present, no industrial hemp extract which can be used for sensitive skin care and no research report of related skin care products which can be used for sensitive skin care and have higher safety to human bodies exist through controlling the content of cannabidiol which can block the action of tetrahydrocannabinol.
Disclosure of Invention
In order to solve the problems, the invention provides an external composition containing industrial hemp extract, which is a preparation prepared by taking the industrial hemp extract as an active ingredient and adding auxiliary materials acceptable in cosmetics; the industrial hemp extract is prepared from the following raw materials in a mass ratio of 5-7: 2-4: 1 industrial hemp leaf, industrial hemp flower and industrial hemp stalk, and extracting with ethanol solution.
Further, the industrial hemp extract is prepared by mixing the following components in a mass ratio of 6: 3: 1 industrial hemp leaf, industrial hemp flower and industrial hemp stalk, and extracting with ethanol solution.
Further, the concentration of the ethanol solution is 50-100%, v/v.
Further, the preparation is water, emulsion, spray, cream, ointment, film or gel.
Furthermore, the preparation consists of the following raw materials in percentage by weight:
3-10% of industrial hemp extract, 2-4% of glycerol, 5-7% of butanediol, 0.05-0.15% of preservative and the balance of deionized water;
or:
3-10% of industrial hemp extract, 13-16% of polyvinyl alcohol PVA, 2.8-4.0% of glycerol, 200.1-0.3% of Tween, 0.04-0.06% of EDTA-2Na, 0.5-1.5% of hyaluronic acid, 0.05-0.15% of preservative and the balance of deionized water;
or:
3-10% of industrial hemp extract, 3.0-4.0% of glycerol, 1-3% of 1, 2-pentanediol, 0.4-0.6% of glycerol stearate, 0.6-0.8% of alkyl glycoside SS, 200.6-0.8% of alkyl glycoside SSE, 1-3% of squalane, 0.5-1.5% of β -glucan, 0.4-0.6% of hyaluronic acid, 0.05-0.15% of preservative and the balance of deionized water.
Furthermore, the preparation consists of the following raw materials in percentage by weight:
5% of industrial hemp extract, 3% of glycerol, 6% of butanediol, 0.1% of preservative and the balance of deionized water;
or:
5% of industrial hemp extract, 15% of polyvinyl alcohol PVA, 3.5% of glycerol, 200.2% of Tween, 0.05% of EDTA-2Na0, 1% of hyaluronic acid, 0.1% of preservative and the balance of deionized water;
or:
0.5 percent of the total weight of the hyaluronic acid, 0.7 percent of the alkyl glycoside SS, 0.7 percent of the alkyl glycoside SSE-200.7 percent of the total weight of the hyaluronic acid, 2 percent of squalane, β -glucan 1 percent, 0.5 percent of hyaluronic acid, 0.1 percent of preservative and the balance of deionized water.
The invention also provides a preparation method of the external composition, which comprises the following steps:
1) weighing raw and auxiliary materials according to the proportion;
2) mixing glycerol, butanediol and deionized water uniformly to obtain phase A; industrial hemp extract as phase B; preservative as phase C;
3) mixing the phase A and the phase B, adding the phase C, and mixing;
or:
1) weighing raw and auxiliary materials according to the proportion;
2) uniformly mixing polyvinyl alcohol (PVA), glycerol, tween 20, EDTA-2Na and deionized water to obtain a phase A; industrial hemp extract and hyaluronic acid as phase B; preservative as phase C;
3) mixing phase A and phase B, adding phase C, mixing, and soaking in facial mask paper for 5-10 min;
or:
1) weighing raw and auxiliary materials according to the proportion;
2) heating and uniformly mixing 1, 2-pentanediol, glycerol and deionized water to obtain phase A, heating and uniformly mixing glycerol stearate, alkyl glycoside SS, alkyl glycoside SSE-20 and squalane to obtain phase B, uniformly mixing industrial hemp extract, β -glucan and hyaluronic acid to obtain phase C, and using preservative as phase D;
3) heating and stirring phase A and phase B at 50-60 deg.C to obtain emulsion, cooling to 35-40 deg.C, sequentially adding phase C and phase D, and mixing.
The invention also provides a preparation method of the industrial hemp extract, which comprises the following steps:
taking leaves, stems and flowers of industrial hemp, adding 10 times (v/w, ml/g) of the industrial hemp, carrying out ultrasonic extraction on the leaves, stems and flowers with 50-100% ethanol aqueous solution for 1.5-2.0h for 2 times in total, and carrying out vacuum concentration at 60 ℃ until the relative density is 1.03 to obtain the hemp extract.
The invention finally provides the application of the external composition in preparing a medicament for treating skin sensitivity.
Further, the skin sensitivity is skin prick, erythema, dryness, desquamation, pain, tightness, itching and/or burning.
Further, the medicament is a medicament for inhibiting hyaluronidase, scavenging free radicals and/or increasing the expression level of skin barrier-related proteins.
Further, the skin barrier associated protein is AQP-3, F L G and/or Caspase-14 protein.
The external composition can inhibit hyaluronidase, remove free radicals, has the effects of resisting inflammation and aging, can improve the expression level of skin barrier-related proteins (AQP-3, F L G and Caspase-14), has a remarkable effect on treating skin sensitivity, is not limited by conventional sensitive skin treatment instruments and professional dermatologists, is convenient to use, has high safety, and has a wide application prospect.
According to the above-mentioned aspects of the present invention, many other modifications, substitutions, or alterations can be made without departing from the basic technical concept of the present invention as defined by the general technical knowledge and common practice in the field relevant to the present invention.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Drawings
FIG. 1 inhibition of hyaluronidase by industrial cannabis extracts
FIG. 2 DPPH radical scavenging ability of Industrial hemp extract
FIG. 3 Effect of Industrial hemp extract on skin Barrier-related protein expression
FIG. 4 Visia images and self-scoring statistics of patients 1 month after the invention was used
Detailed Description
The raw materials and equipment used in the embodiment of the present invention are known products and obtained by purchasing commercially available products.
Example 1 preparation of Industrial hemp extract of the invention
Mixing 600g of industrial hemp leaves, 300g of industrial hemp stems and 100g of industrial hemp flowers, adding 10L 50% ethanol water solution, performing ultrasonic extraction for 1.5h for 2 times, mixing the extractive solutions, concentrating at 60 deg.C, and drying under reduced pressure to-0.10 MPa to relative density of 1.03.
Example 2 preparation of Industrial hemp extract of the invention
Mixing 300g of industrial hemp leaves, 150g of industrial hemp stems and 50g of industrial hemp flowers, adding 5L 70% ethanol water solution, carrying out ultrasonic extraction for 2.0h, carrying out extraction for 2 times, combining the extracting solutions, concentrating at 60 ℃, and drying under reduced pressure of-0.10 MPa until the relative density is 1.03.
Example 3 preparation of Industrial hemp extract of the invention
Mixing 1200g of industrial hemp leaves, 600g of industrial hemp stems and 200g of industrial hemp flowers, adding 20L absolute ethyl alcohol, carrying out ultrasonic extraction for 1.8h, carrying out extraction for 2 times, combining the extracting solutions, concentrating at 60 ℃, and drying under reduced pressure of-0.10 MPa until the relative density is 1.03.
Example 4 facial mask containing Industrial hemp extract
The formula is as follows:
the preparation method comprises the following steps: respectively weighing the A phase and the B phase, respectively mixing uniformly, mixing the A phase and the B phase, finally adding the C phase, and then soaking for 5-10min by using facial mask paper to obtain the facial mask.
The using method comprises the following steps: directly applying the facial mask to face, and standing for 15-20 min.
Example 5 spray containing Industrial hemp extract
The formula is as follows:
the preparation method comprises the following steps: respectively weighing the A phase and the B phase, respectively mixing uniformly, mixing the A phase and the B phase, finally adding the C phase, and mixing uniformly to obtain the composition.
The using method comprises the following steps: it can be sprayed directly on face or other sensitive skin parts.
Example 6 emulsion containing Industrial hemp extract
The formula is as follows:
the preparation method comprises the following steps: respectively weighing the A phase and the B phase, respectively heating and mixing uniformly, then mixing the A phase and the B phase, heating and stirring until the A phase and the B phase are emulsion-shaped, adding the C phase and mixing uniformly when the temperature is reduced to 35-40 ℃, and finally adding the D phase and mixing uniformly.
The using method comprises the following steps: after the skin is cleaned, the lotion is directly applied to sensitive skin.
The beneficial effects of the invention are further illustrated by way of experimental examples as follows:
test example 1 analysis of the composition of industrial hemp extract and efficacy verification
(1) Analysis of Industrial hemp extract Components
The industrial hemp extract prepared according to example 1 was stored at low temperature in the dark for the analysis of the components.
The extract was identified by high performance liquid chromatography (HP L C) under separation conditions in which Cannabidiol (CBD) was obtained as the main active ingredient, using cannabidiol as a standard control, and the separation conditions were Inertsoil ODS-3(150mm × 4.6.6 mm × 5 μm), mobile phase (methanol: water, 80: 20, v/v), flow rate 1.0m L/min, UV detector wavelength 220nm, column temperature 35 ℃ and the results are shown in Table 1.
TABLE 1 determination of cannabidiol content
| Name of substance | Target object to be measured | Content (mg/g) |
| Industrial hemp extract | Cannabidiol (CBD) | 1.3～2.9 |
(2) Cell safety experiments on industrial hemp extracts
The application of any substance must be safe firstly, and the efficacy of the substance can be discussed only on the premise of safety, and the experiment researches the safety of the industrial hemp extract to human skin keratinocytes.
The specific operation is that the skin keratinocyte HaCaT growing to about 80 percent in a culture dish is recovered into a centrifuge tube in a sterile operation platform, counted by a cell counter according to the counting of 2.0-8.0 × 104cells/well were seeded into 24-well cell culture plates and cultured for 24 h. Industrial hemp extract (prepared in example 1) was formulated to the corresponding concentration and sterilized by filtration through 0.22 μm filter. Adding a sample to be detected with a target concentration into the HaCaT cells, and continuously culturing for 24h in an incubator at 37 ℃. After adding a proper amount of CCK-8 liquid into the culture plate, continuously culturing for 4h, measuring the absorbance at 450nm, and calculating the survival rate of the cells under the action of each concentration, wherein the specific results are shown in Table 2.
TABLE 2 HaCaT cell survival rate from Industrial hemp extract
The results show that: the industrial hemp extract is safe to HaCaT cells at a concentration of not more than 5% by mass.
(3) Hyaluronic acid inhibition rate of industrial hemp extract
Hyaluronic Acid (HA) is the largest component in extracellular matrix (ECM) with the largest content and proportion, HAs strong water absorption capacity and adhesion, can regulate the secretion of cytokines, and influences the growth, proliferation, migration and differentiation of cells, thereby playing a main role in the processes of maintaining the moisture and elasticity of skin, healing wounds, forming blood vessels and the like. Hyaluronidase is a specific lyase of hyaluronic acid, and inhibition of hyaluronidase activity can ensure the content and normal function of hyaluronic acid. The hyaluronidase inhibition experiment is the most typical in vitro method for evaluating the anti-allergic activity, the anti-allergic activity of a substance is evaluated by taking the hyaluronidase inhibition rate as an index, and the higher the hyaluronidase inhibition rate is, the stronger the anti-allergic activity is.
The hyaluronidase is prepared in situ with acetic acid buffer solution (500U/M L), sodium hyaluronate (0.5mg/M L), acetylacetone solution 50M L, P-DAB chromogenic reagent (0.8g P-dimethylaminobenzaldehyde dissolved in 15M L concentrated hydrochloric acid and 15M L2 absolute ethyl alcohol), CaCl2(2.5M), NaOH (5M) is prepared, the industrial hemp extract (prepared in example 1) is assayed with hyaluronidase by Elson-Morgan modification, briefly, 0.5M L sample/distilled water is added with 0.5M L U/M855 hyaluronidase, incubated at 37 ℃ for 20min, 0.1M L CaCl2(2.5M), incubated again at 37 ℃ for 20min, then the sample and distilled water are added with 0.5M L sodium hyaluronate (0.5mg/M L), another 0.5M L pH is added, the buffer solution is incubated at 37 min, the temperature is adjusted to 585 min, the pH is added to 0.10 min, the sample and the distilled water tubes are then incubated at room temperature for 0.10 min, the absorbance is assayed with 0.5M NaOH solution, and the pH is added with 0.5M acetone solution, and the final water bath is added with 0.5M acetone solution, the absorbance is added with 0.5M acetone solution, the pH is added with 0.5M.
As can be seen from fig. 1: the industrial hemp extract has hyaluronidase inhibition effect in the mass concentration range of 0.001% -1%, and the effect increases with increasing concentration, and when the concentration is increased to 5%, the hyaluronidase inhibition rate reaches 72% at maximum, and then the hyaluronidase inhibition effect is stable.
(4) Free radical scavenging rate of industrial hemp extract
The inhibition of free radical activity is effective in alleviating the degradation of cell structure function under the action of excess free radicals generated after external stimulation, thereby maintaining the normal barrier structure of various organs including the skin, and the like, using DPPH scavenging test, which is briefly described as follows, 2.0M L60 μ M ethanol DPPH solution is taken in a 10M L stoppered tube, 3M L5 mg/M L sample solution is added, the reaction is closed for 30min at room temperature, the absorbance is measured at 517nm, the clearance is calculated, the absorbance is measured with water instead of DPPH, and the absorbance is measured with water instead of DPPH and the sample instead of DPPH, and the DPPH free radical scavenging ability of the industrial cannabis extract (prepared in example 1) is measured, and the specific results are shown in FIG. 2.
As can be seen from fig. 2: in the mass concentration range of 0.01-2.5%, the scavenging rate of DPPH free radical of industrial hemp extract is gradually increased, and the industrial hemp extract has linear correlation. When the concentration is gradually higher than 2.5%, the inhibition rate of DPPH free radicals begins to decrease.
(5) Effect of Industrial hemp extract on expression of skin Barrier-related structural proteins
AQP-3 is involved in the absorption and secretion of substances of cells and contributes to elastic maintenance and damage repair, filaggrin (F L G) is a matrix protein among filaments of cells constituting a eukaryotic cell skeleton, the formation of an intercellular cross-linked membrane involved in terminal differentiation of keratinocytes, natural moisturizing factors generated by decomposition are very important for skin hydration and skin barrier, pase-14 is involved in terminal differentiation of keratinocytes, pro-F L G in the granular layer is rapidly phosphorylated by Caspase-14 to F L G to form an intact stratum corneum, protein blotting (Western blot, WB) is used for measuring the expression of proteins of AQP-3, Caspase-14 and F L G, the experimental method comprises the steps of collecting cells, using ice-crack resuspension suspension cell precipitation, using BCA kit to measure protein concentration, subjecting cell lysate to gel electrophoresis, adding immune imprinted protein to a PVDF Caspase membrane, adding the AQP-3, performing gradient light-washing analysis, using a light-washing solution to prepare a dry image, using a dry-wash solution after the supernatant of protein is collected, using a dry-gel electrophoresis, using a dry-wash solution to measure the protein concentration of BCA 7 protein, using a dry-wash solution after the supernatant of the cell lysate is collected, and the supernatant is analyzed by using a dry-wash solution after the supernatant of a dry-wash solution, the supernatant of the protein is analyzed by using a dry protein, the supernatant of the protein, the supernatant of the protein, the.
As can be seen from figure 3, the industrial hemp extract is helpful for improving the expression level of AQP-3, Caspase-14 and F L G proteins related to the skin barrier, recovering the damage of external stimuli (such as ultraviolet light, air pollution and the like) to the skin barrier, enhancing the water locking, moisturizing and other performances of the skin barrier, repairing the damaged skin stratum corneum and having good recovery effect on sensitive skin.
(6) Influence of industrial hemp extract on TRPV family gene expression level
According to the existing report, TRPV4 protein forms a pentagonal structure together with β -catenin and cadherin in epidermal keratinocytes of mice, and the interaction plays an important role in connection of the keratinocytes and further plays an important role in the health condition of skin barriers.
HaCaT cells were first cultured in medium containing 0.04mM calcium (low calcium medium) withThen transferred to a medium added with 1.5mM CaCl2In the medium (high calcium medium), HaCaT cells are induced to differentiate by a high calcium switch. Subsequently, the cell culture after the induction of differentiation by hypercalcemic switch was maintained at 33 ℃ (physiological temperature of skin) or 28 ℃ (threshold temperature) for 24 h. The industrial cannabis extracts (prepared in example 1) were added separately and the change in the expression level of TRPV4 was observed. The expression results are shown in Table 3.
The sequence of TRPV4 was designed for determining the gene expression level of TRPV4, and the details are as follows:
TRPV4 R：5’-CGTGAGAACACCAAGTTTGTTAC-3’
TRPV4 L：5’-CTTGCTGTTGTACACCAGGATC-3’
GAPDH R：5’-TGAAGGTCGGAGTCAACGGATTTGT-3’
GAPDH L：5’-CATGTGGGCCATGAGGTCCACCAC-3’
TABLE 3 relative expression values of industrial hemp extracts on TRPV4 gene
As can be seen from Table 3, the industrial cannabis extracts have inhibitory effects on the expression of TPRV4 by HaCaT cells stimulated by the external temperature environment, and are presumed to have a repairing effect on sensitive skin.
Test example 3 clinical efficacy verification of composition containing industrial hemp extract
The series of facial masks, sprays and lotions prepared in examples 4, 5 and 6 were administered to 30 sensitive skin patients (diagnosed and confirmed by clinical dermatologists) and self-evaluated for dimensions such as stinging, erythema, dry skin, desquamation, pain, tightness, pruritus and perm, with a score of 0-5 (0 points representing no stinging, no erythema, low dryness of skin, no desquamation, no pain, no tightness, no pruritus symptom and no perm, and gradually increasing in degree from 0 to 5 points). The sensitive skin patients self-score according to the scoring table before using the facial mask product, then use the facial mask product, respectively score 15min, 1 day, 3 days, 5 days, 15 days and 30 days after using the facial mask,
typical effects are shown in fig. 4, and according to results, the facial prickling, erythema, dry skin, desquamation, pain, tightness, pruritus and perm of sensitive skin patients are obviously improved after the industrial hemp extract facial mask is used.
In conclusion, the external composition disclosed by the invention can inhibit hyaluronidase, remove free radicals, improve the expression level of skin barrier-related proteins (AQP-3, F L G and Caspase-14), has a remarkable effect on treating skin sensitivity, is not limited by conventional sensitive skin treatment instruments and professional dermatologists, is convenient to use and high in safety, and has a wide application prospect.
Claims (12)
1. A composition for external use containing an extract of industrial hemp, characterized in that: the preparation is prepared by taking industrial hemp extract as an active ingredient and adding auxiliary materials acceptable in cosmetics; the industrial hemp extract is prepared from the following raw materials in a mass ratio of 5-7: 2-4: 1 industrial hemp leaf, industrial hemp flower and industrial hemp stalk, and extracting with ethanol solution.
2. The composition for external use according to claim 1, wherein: the industrial hemp extract is prepared from the following raw materials in a mass ratio of 6: 3: 1 industrial hemp leaf, industrial hemp flower and industrial hemp stalk, and extracting with ethanol solution.
3. The composition for external use according to claim 1, wherein: the concentration of the ethanol solution is 50-100%, v/v.
4. The composition for external use according to any one of claims 1 to 3, characterized in that: the preparation is water agent, emulsion, spray, cream, ointment, pellicle or gel.
5. The topical composition according to claim 4, characterized in that: the preparation is prepared from the following raw materials in percentage by weight:
3-10% of industrial hemp extract, 2-4% of glycerol, 5-7% of butanediol, 0.05-0.15% of preservative and the balance of deionized water;
or:
3-10% of industrial hemp extract, 13-16% of polyvinyl alcohol PVA, 2.8-4.0% of glycerol, 200.1-0.3% of Tween, 0.04-0.06% of EDTA-2Na, 0.5-1.5% of hyaluronic acid, 0.05-0.15% of preservative and the balance of deionized water;
or:
3-10% of industrial hemp extract, 3.0-4.0% of glycerol, 1-3% of 1, 2-pentanediol, 0.4-0.6% of glycerol stearate, 0.6-0.8% of alkyl glycoside SS, 200.6-0.8% of alkyl glycoside SSE, 1-3% of squalane, 0.5-1.5% of β -glucan, 0.4-0.6% of hyaluronic acid, 0.05-0.15% of preservative and the balance of deionized water.
6. The composition for external use according to claim 5, wherein: the preparation is prepared from the following raw materials in percentage by weight:
5% of industrial hemp extract, 3% of glycerol, 6% of butanediol, 0.1% of preservative and the balance of deionized water;
or:
5% of industrial hemp extract, 15% of polyvinyl alcohol PVA, 3.5% of glycerol, 200.2% of Tween, 0.05% of EDTA-2Na, 1% of hyaluronic acid, 0.1% of preservative and the balance of deionized water;
or:
5% of industrial hemp extract, 3.5% of glycerol, 2% of 1, 2-pentanediol, 0.5% of glycerol stearate, 0.7% of alkyl glycoside SS, 200.7% of alkyl glycoside SSE, 2% of squalane, β -glucan 1%, 0.5% of hyaluronic acid, 0.1% of preservative and the balance of deionized water.
7. A process for preparing the composition for external use according to claim 5 or 6, characterized in that; it comprises the following steps:
1) weighing raw and auxiliary materials according to the proportion;
2) mixing glycerol, butanediol and deionized water uniformly to obtain phase A; industrial hemp extract as phase B; preservative as phase C;
3) mixing the phase A and the phase B, adding the phase C, and mixing;
or:
1) weighing raw and auxiliary materials according to the proportion;
2) uniformly mixing polyvinyl alcohol (PVA), glycerol, tween 20, EDTA-2Na and deionized water to obtain a phase A; industrial hemp extract and hyaluronic acid as phase B; preservative as phase C;
3) mixing phase A and phase B, adding phase C, mixing, and soaking in facial mask paper for 5-10 min;
or:
1) weighing raw and auxiliary materials according to the proportion;
2) heating and uniformly mixing 1, 2-pentanediol, glycerol and deionized water to obtain phase A, heating and uniformly mixing glycerol stearate, alkyl glycoside SS, alkyl glycoside SSE-20 and squalane to obtain phase B, uniformly mixing industrial hemp extract, β -glucan and hyaluronic acid to obtain phase C, and using preservative as phase D;
3) heating and stirring phase A and phase B at 50-60 deg.C to obtain emulsion, cooling to 35-40 deg.C, sequentially adding phase C and phase D, and mixing.
8. A method of preparing an extract of industrial cannabis sativa according to any of claims 1 to 4, characterized in that it comprises the following steps:
weighing leaves, stems and flowers of cannabis sativa according to a ratio, adding 10 times (v/w, ml/g) of 50-100% ethanol aqueous solution, performing ultrasonic extraction for 1.5-2.0h for 2 times in total, combining the extracting solutions, and performing vacuum concentration at 60 ℃ until the relative density is 1.03 to obtain the cannabis sativa extract.
9. Use of a topical composition according to any one of claims 1 to 6 in the manufacture of a medicament for the treatment of skin sensitivity.
10. Use according to claim 9, characterized in that the skin sensitivity is skin prick, erythema, dryness, desquamation, pain, tightness, itching and/or perm.
11. The use according to claim 9, wherein the medicament is a medicament for inhibiting hyaluronidase, scavenging free radicals and/or increasing the amount of expression of skin barrier-related proteins.
12. Use according to claim 11, wherein said skin barrier associated protein is AQP-3, F L G and/or Caspase-14 protein.
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