CN111494306A - Female private gel microecological preparation composition and preparation method thereof - Google Patents
Female private gel microecological preparation composition and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a female private gel microecological preparation composition and a preparation method thereof, wherein the female private gel microecological preparation composition comprises the following components in percentage by mass: 3.0-5.0% of glycerol, 5.0-10.0% of butanediol, 3.0-5.0% of poloxamer, 2.0-4.0% of lactobacillus rhamnosus fermentation product filtrate, 1.0-2.0% of theaflavin extracting solution, 1.0-2.0% of dictyophora fungus skirt extracting solution, 0.3-0.8% of lactic acid, 0.2-0.4% of allantoin, 0.1-0.2% of EDTA disodium, 0.0001-0.1% of carbonized polyamine nano particle solution and 84.3999-70.5% of deionized water. The composition has better antibacterial effect, small irritation to vaginal skin and high safety performance, and has the effects of moisturizing, improving pudendum labial pigment fading, improving pudendum vaginal pruritus, improving pudendum vaginal odor and the like.
Description
Technical Field
The invention belongs to the technical field of female private nursing, and particularly relates to a female private gel microecological preparation composition and a preparation method thereof.
Background
The health of the female vagina is maintained by a plurality of symbiotic floras, the symbiotic floras respectively play different mechanisms to protect the vagina from entering of pathogenic microorganisms, when the balance of the symbiotic floras is destroyed, the problems of abnormal secretion increase, harmful floras hyperplasia and the like can be caused, and the female diseases are easy to generate, so the vaginal flora is an important factor for ensuring the health of the vagina for maintaining the balance of the symbiotic bacteria and the weak acid environment of the vagina, and in addition, the vaginal flora plays an effective antibacterial and anti-inflammatory function and supplements beneficial lactobacillus filtrate for the infection and inflammation phenomenon of the vagina caused by menstrual cycle disorder and improper cleaning, keeps the moist and dry environment of the vagina and enables the pudendum to recover the normal symbiotic floras state, which is also an important issue.
With the increase of the working pressure of modern society, female endocrine disorder is easily caused, menstrual disorder and immunity are reduced, vaginal parts are easily blocked and infected, and various private care products are commercially available for solving the health problem of the female vaginal parts, and are basically chemically synthesized bacteriostatic agents which can generate strong stimulation to the vaginal mucosa parts.
In order to solve the problems, the Chinese patent application with the patent number of CN107028978A provides a female vaginal gel, which is prepared from the following raw materials in parts by weight: 150 portions of nano-bubble hydrogen-rich water with the pH value of 4.0-7.5, 3000 portions of artificial ionized acidic oxidation potential water with the pH value of 2.0-4.0 and 30-330 portions of aqueous gel matrix; the aqueous gel matrix comprises: water, glycerol, propylene glycol, triethanolamine, cellulose derivatives, carbomer, poloxamer, carrageenan, chitosan and alginate, tragacanth, pectin, gelatin, xanthan gum, agar, non-cellulose polysaccharide, vinyl polymer, acrylic resin, polyvinyl alcohol, carbopol and oily gel in any proportion. The female vaginal gel mainly promotes the growth of probiotics and resists the invasion of external harmful flora by adjusting the pH value of the female vaginal part, however, the pH value of the female vaginal part is closely related to the age, the bacteriostatic effect is not obvious only by adjusting the pH value of the vaginal part, and the inflammation is easy to relapse after the use of the gel is stopped; in addition, the gelling agent only plays a role in bacteriostasis and inflammation diminishing, has no fading effect on melanin sediment of the pudendum and has no moisturizing effect.
Disclosure of Invention
The invention aims to provide a female private gel microecological preparation composition and a preparation method thereof, aiming at the problems that a gelling agent in the prior art has poor antibacterial effect on female private parts, can not fade melanin and the like.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention relates to a female private gel microecological preparation composition which comprises the following components in percentage by mass: 3.0-5.0% of glycerol, 5.0-10.0% of butanediol, 3.0-5.0% of poloxamer, 2.0-4.0% of lactobacillus rhamnosus fermentation product filtrate, 1.0-2.0% of theaflavin extracting solution, 1.0-2.0% of dictyophora fungus skirt extracting solution, 0.3-0.8% of lactic acid, 0.2-0.4% of allantoin, 0.1-0.2% of EDTA disodium, 0.0001-0.1% of carbonized polyamine nano particle solution and 84.3999-70.5% of deionized water.
Preferably, the total mass percentage of the lactobacillus rhamnosus fermentation product filtrate, the theaflavin extract, the dictyophora indusiata skirt extract and the carbonized polyamine nanoparticle solution is 7.6%.
Preferably, the weight percentages of the components are as follows: 5.0% of glycerol, 10.0% of butanediol, 4.5% of poloxamer, 3.8% of lactobacillus rhamnosus fermentation product filtrate, 1.8% of theaflavin extracting solution, 1.9% of dictyophora phalloidea sheel extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium, 0.1% of carbonized polyamine nano particle solution and 71.77% of deionized water.
The invention also relates to a preparation method of the female private gel microecological preparation composition, which comprises the following steps:
1) mixing glycerol, butanediol, allantoin, EDTA disodium and deionized water, heating and stirring until the mixture is completely dispersed and dissolved to form a water phase;
2) pouring poloxamer and the water phase into a vacuum homogenizing stirring pot for vacuum homogenizing stirring;
3) sequentially adding lactobacillus rhamnosus fermentation product filtrate, dictyophora indusiata skirt extracting solution, theaflavin extracting solution and carbonized polyamine nano particle solution, and continuing to carry out vacuum homogenization and stirring;
4) adding lactic acid to adjust pH value, and pressure filtering to obtain the female private gel microecological preparation composition.
Preferably, the theaflavin extract in step 3) is extracted from dried and sieved srilanka black tea powder by the following steps: putting black tea powder into a reaction kettle, putting the reaction kettle into a counter-current ultrasonic extractor, adding denatured ethanol-water solution for extraction, and filtering until no impurity precipitates after the extraction is finished.
Preferably, the concentration of the denatured ethanol-water solution is 62%; in the extraction process of the theaflavin extract, the ultrasonic power is 600W, the stirring speed is 600rpm, the temperature is controlled at 65 ℃, and the extraction time is 40 mins.
Preferably, the dictyophora phalloidea skirt extract in the step 3) is extracted from dried, crushed and sieved dictyophora phalloidea skirt powder, and the extraction steps are as follows: putting the dictyophora fungus skirt powder into a reaction kettle, putting the reaction kettle into a counter-current ultrasonic extractor, adding a modified ethanol-water solution for extraction, and filtering until no impurity precipitate is generated after the extraction is finished.
Preferably, the concentration of the denatured ethanol-water solution is 60%; in the extraction process of the dictyophora phalloidea skirt extract, the ultrasonic power is 700W, the stirring speed is 600rpm, the temperature is controlled at 55 ℃, and the extraction time is 50 mins.
Preferably, in the step 2), the specific step of homogenizing and stirring includes:
2.1) pouring the poloxamer and the water phase into a vacuum homogenizing and stirring pot, and then starting a homogenizing and stirring blade at the stirring speed of 600rpm and the homogenizing speed of 2000 rpm;
2.2) vacuumizing to 0.04Mpa, and continuing homogenizing and stirring for 15Mins under the vacuum state;
2.3) stirring in vacuum and cooling to 40 ℃;
the specific steps of the step 3) comprise:
3.1) after the vacuum pressure is released, sequentially adding lactobacillus rhamnosus fermentation product filtrate, dictyophora phalloidea skirt extracting solution, theaflavin extracting solution and polyamine carbide nano particle solution;
3.2) vacuumizing again, and homogenizing and stirring under the vacuum condition, wherein the stirring speed is 600rpm, the homogenizing speed is 2000rpm, and the homogenizing and stirring time is 10 Mins;
3.3) under the vacuum condition, continuously stirring and cooling to 30 ℃.
Preferably, the heating in the step 1) is carried out to 80 ℃; and in the step 4), the pH value is adjusted to 4.0-4.5, and a stainless steel filter screen of 80 meshes is used for pressure filtration.
Compared with the prior art, the technical scheme provided by the invention has the following beneficial effects:
1. the female private gel microecological preparation composition provided by the invention is added with the lactobacillus rhamnosus fermentation product filtrate, the lactobacillus rhamnosus fermentation product filtrate belongs to gram-positive probiotics, exists in human intestinal tracts, and has the characteristics of anaerobism and acid resistance.
2. The female private gel microecological preparation composition provided by the invention is added with the dictyophora phalloidea skirt extracting solution, is a natural cryptosporidium parasitized at the root of dried bamboo, has the medicinal effect similar to ginseng, is rich in dictyophora phalloidea polysaccharide, alkaloid, colloid fiber and the like, has the effects of high-efficiency oxidation resistance, moisture preservation and inflammation diminishing, and is beneficial to enhancing the effects of vaginal oxidation resistance and maintaining refreshing and moisture preservation due to the addition of the raw materials.
3. The female private gel microecological preparation composition related by the invention is added with theaflavin extract which is obtained by fermenting the red tea leaves of srilanca, is a compound with polyphenol hydroxyl group having a theanophenol ketone structure and pharmacological action, is rich in a large amount of polyphenols and catechin, has strong effects of resisting oxidation and removing free radicals, has a certain inhibition effect on inhibiting bad bacteria proliferated in the vaginal environment, and has a defense function.
4. The female private gel microecological preparation composition provided by the invention is added with a carbonized polyamine nanoparticle solution, natural herbal activated carbon synthesized by biological polyamine molecule spermidine is utilized, the main antibacterial mechanism is that bacterial cell membrane rupture and bacterial metabolism function influence are caused by high positive charge polyamine biochar, the minimum inhibitory concentration is nearly 2500 times lower, and infected fungi can be completely eliminated within 3 days.
5. The invention also adds poloxamer which is a nonionic surfactant, has thickening function and emulsification assisting function, has no irritation and does not contain benzene ring residue, and eliminates the anionic carbomer thickener used in most of commercial products in the market.
6. The lactic acid used in the invention is used as a stabilizer for adjusting the pH value, is controlled within the range of 4.0-4.5, conforms to the weak acid environment of vagina and enables the active substances to play the maximum self-function.
Detailed Description
For further understanding of the present invention, the present invention will be described in detail with reference to examples, which are provided for illustration of the present invention but are not intended to limit the scope of the present invention.
Example 1
The female private gel microecological preparation composition related to the embodiment comprises the following components in percentage by mass: 3.0% of glycerol, 7.0% of butanediol, 3.5% of poloxamer, 2.0% of lactobacillus rhamnosus fermentation product filtrate, 1.2% of theaflavin extracting solution, 1.3% of dictyophora phalloidea skirt extracting solution, 0.4% of lactic acid, 0.2% of allantoin, 0.1% of EDTA disodium, 0.001% of carbonized polyamine nano-particle solution and 81.299% of deionized water.
The preparation method of the female private gel microecological preparation composition comprises the following steps:
1) mixing glycerol, butanediol, allantoin, EDTA disodium and deionized water, heating to 80 ℃, and stirring until the mixture is completely dispersed and dissolved to form a water phase;
2) weighing poloxamer, pouring the poloxamer and the water phase into a vacuum homogenizing stirring pot for vacuum homogenizing stirring, and the specific steps are as follows:
2.1) weighing poloxamer, pouring the poloxamer and the water phase into a vacuum homogenizing and stirring pot, and then starting a homogenizing and stirring blade at the stirring speed of 600rpm and the homogenizing speed of 2000 rpm;
2.2) vacuumizing to 0.04Mpa, and continuing homogenizing and stirring for 15Mins under the vacuum state;
2.3) stirring in vacuum and cooling to 40 ℃.
3) Sequentially adding lactobacillus rhamnosus fermentation product filtrate, dictyophora indusiata skirt extracting solution, theaflavin extracting solution and carbonized polyamine nano particle solution, and continuously carrying out vacuum homogenization stirring, wherein the specific steps are as follows:
3.1) after the vacuum pressure is released, sequentially adding lactobacillus rhamnosus fermentation product filtrate, dictyophora phalloidea skirt extracting solution, theaflavin extracting solution and polyamine carbide nano particle solution;
3.2) vacuumizing again, and homogenizing and stirring under the vacuum condition, wherein the stirring speed is 600rpm, the homogenizing speed is 2000rpm, and the homogenizing and stirring time is 10 Mins;
3.3) under the vacuum condition, continuously stirring and cooling to 30 ℃;
4) adding lactic acid to adjust the pH value to 4.0-4.5, and then performing pressure filtration by using a stainless steel filter screen of 80 meshes to obtain the female private gel microecological preparation composition.
The theaflavin extract used in the step 3) is extracted from dried and sieved srilanka black tea powder, and the extraction steps are as follows: putting 8g of black tea powder into a reaction kettle, putting the reaction kettle into a counter-current ultrasonic extractor, adding 65ml of denatured ethanol-water solution for extraction, controlling the concentration of the denatured ethanol-water solution to be 62%, the ultrasonic power to be 600W, the stirring speed to be 600rpm, the temperature to be 65 ℃, the extraction time to be 40mins, and filtering until no impurity precipitates are generated after the extraction is finished.
The dictyophora phalloidea skirt extract used in the step 3) is extracted from the dictyophora phalloidea skirt powder which is dried, crushed and sieved, and the extraction steps are as follows: putting 10g of dictyophora phalloidea powder into a reaction kettle, putting the reaction kettle into a counter-current ultrasonic extractor, adding 80ml of denatured ethanol-water solution for extraction, wherein the concentration of the denatured ethanol-water solution is 60%, the ultrasonic power is 700W, the stirring speed is 600rpm, the temperature is controlled at 55 ℃, the extraction time is 50mins, and after the extraction is finished, filtering until no impurity precipitates are generated.
Example 2
The female private gel microecological preparation composition related to the embodiment comprises the following components in percentage by mass: 4.0% of glycerol, 8.0% of butanediol, 4.0% of poloxamer, 3.0% of lactobacillus rhamnosus fermentation product filtrate, 1.5% of theaflavin extracting solution, 1.6% of dictyophora phalloidea sheel extracting solution, 0.8% of lactic acid, 0.3% of allantoin, 0.15% of EDTA disodium, 0.05% of carbonized polyamine nano particle solution and 76.65% of deionized water.
The preparation method of the female private gel microecological preparation composition related to the present example is the same as that of the first example, and the description of the present example is omitted.
Example 3
The female private gel microecological preparation composition related to the embodiment comprises the following components in percentage by mass: 5.0% of glycerol, 10.0% of butanediol, 4.5% of poloxamer, 3.8% of lactobacillus rhamnosus fermentation product filtrate, 1.8% of theaflavin extracting solution, 1.9% of dictyophora phalloidea sheel extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium, 0.1% of carbonized polyamine nano particle solution and 71.77% of deionized water.
The preparation method of the female private gel microecological preparation composition related to the present example is the same as that of the first example, and the description of the present example is omitted.
Example 4
The female private gel microecological preparation composition related to the embodiment comprises the following components in percentage by mass: 5.0% of glycerol, 10.0% of butanediol, 5.0% of poloxamer, 4.0% of lactobacillus rhamnosus fermentation product filtrate, 2.0% of theaflavin extracting solution, 2.0% of dictyophora phalloidea sheel extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium, 0.1% of carbonized polyamine nano-particle solution and 70.77% of deionized water.
The preparation method of the female private gel microecological preparation composition related to the present example is the same as that of the first example, and the description of the present example is omitted.
Efficacy test 1
Candida albicans is a resident strain in the vagina, and many external factors can cause the strain to excessively proliferate, so that the balance of symbiotic flora in the vagina is damaged, pruritus vulvae and inflammation of the vagina are caused, and the efficacy experiment is used for carrying out an antibacterial challenge experiment of the Candida albicans and testing the antibacterial ability of the female private gel microecological preparation composition related to the examples 1-4.
The efficacy experiment adopts a blank group 1, a positive control group 2, a normal saline group 3 and treatment groups 4-7, wherein the treatment groups 4-7 respectively aim at the condensation test of embodiments 1-4 and inoculate candida albicans by using test tubes.
Blank group 1: preparing 15ml of a Sabouraud's dextrose liquid culture medium;
positive control group 2: inoculating 0.5ml of Candida albicans with 1 × 106 strain number to 15ml of glucose liquid culture medium, placing in a constant temperature shaking incubator, and culturing at 48Hrs with the temperature of 37 ℃ and the oscillation frequency of 250 rpm;
saline group 3: inoculating 0.5ml of 1 × 106 strain number Candida albicans in 15ml of Sabouraud's glucose liquid culture medium, adding 0.5ml of physiological saline, placing in a constant temperature shaking incubator, setting the temperature to be 37 ℃, the oscillation frequency to be 250rpm, and culturing to be 48 Hrs;
treatment group 4: taking 0.5ml of Candida albicans with the strain number of 1 × 106, inoculating the Candida albicans in 15ml of Sasa dextrose liquid culture medium, then adding 0.5ml of the female private gel microecological preparation composition related to the embodiment 1, placing the mixture in a constant temperature shaking table incubator, setting the temperature to be 37 ℃, the oscillation frequency to be 250rpm, and culturing the mixture to be 48 Hrs;
treatment group 5: taking 0.5ml of Candida albicans with the strain number of 1 × 106, inoculating the Candida albicans in 15ml of Sasa dextrose liquid culture medium, then adding 0.5ml of the female private gel microecological preparation composition related to the embodiment 2, placing the mixture in a constant temperature shaking table incubator, setting the temperature to be 37 ℃, the oscillation frequency to be 250rpm, and culturing the mixture to be 48 Hrs;
treatment group 6: taking 0.5ml of Candida albicans with the strain number of 1 × 106, inoculating the Candida albicans in 15ml of Sasa glucose liquid culture medium, adding 0.5ml of the female private gel microecological preparation composition related to the embodiment 3, placing the mixture in a constant temperature shaking table incubator, setting the temperature to be 37 ℃, the oscillation frequency to be 250rpm, and culturing the mixture to be 48 Hrs;
treatment group 7: 0.5ml of Candida albicans (1X 106 strain number) was inoculated into 15ml of a Sabouraud's dextrose broth, 0.5ml of the female private gel microecological preparation composition of example 4 was added, and the mixture was placed in a constant temperature shaking incubator and cultured at 37 ℃ and an oscillation frequency of 250rpm of 48 Hrs.
The test results are shown in table 1:
table 1: candida albicans inhibition test structure
In Table 1, "+" indicates pass "-" indicates fail.
Summary of the assay: the experimental result shows that the appearance of the positive control group 2 is turbid white and represents a long Candida albicans growth state, the appearance of the invention treatment groups 4, 5, 6 and 7 and the appearance of the blank group 1 and the physiological saline group 3 are all transparent, which represents that the growth of the inoculated Candida albicans is completely and effectively inhibited, and the experiment shows that the minimum inhibition Candida albicans concentration of the treatment groups 4-7 of the invention reaches the standard.
Comparative example 1
The female private gel microecological preparation composition related to the comparative example comprises the following components in percentage by mass: 5.0% of glycerin, 10.0% of butanediol, 4.5% of poloxamer, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium and 79.37% of deionized water.
Compared with the embodiment 3, the lactobacillus rhamnosus fermentation product filtrate, the theaflavin extracting solution, the dictyophora fungus skirt extracting solution and the carbonized polyamine nano particle solution are not added, and the deionized water with corresponding mass parts is supplemented.
Comparative example 2
The female private gel microecological preparation composition related to the comparative example comprises the following components in percentage by mass: 5.0% of glycerol, 10.0% of butanediol, 4.5% of poloxamer, 1.8% of theaflavin extracting solution, 1.9% of dictyophora fungus skirt extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium, 0.1% of carbonized polyamine nano particle solution and 75.57% of deionized water.
Compared with the embodiment 3, the comparative example does not add the filtrate of the fermentation product of the lactobacillus rhamnosus, and supplements deionized water with corresponding mass parts.
Comparative example 3
The comparative example relates to a female private gel microecological preparation composition which comprises the following components in percentage by mass: 5.0% of glycerol, 10.0% of butanediol, 4.5% of poloxamer, 3.8% of lactobacillus rhamnosus fermentation product filtrate, 1.9% of dictyophora phalloidea skirt extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium, 0.1% of carbonized polyamine nano particle solution and 73.57% of deionized water.
Compared with the example 3, the comparative example does not add theaflavin extract and is supplemented with corresponding parts by mass of deionized water.
Comparative example 4
The comparative example relates to a female private gel microecological preparation composition which comprises the following components in percentage by mass: 5.0% of glycerol, 10.0% of butanediol, 4.5% of poloxamer, 3.8% of lactobacillus rhamnosus fermentation product filtrate, 1.8% of theaflavin extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium, 0.1% of carbonized polyamine nano particle solution and 74.57% of deionized water.
Compared with the embodiment 3, the bamboo fungus skirt extracting solution is not added, and the deionized water with corresponding mass parts is supplemented.
Comparative example 5
The comparative example relates to a female private gel microecological preparation composition which comprises the following components in percentage by mass: 5.0% of glycerol, 10.0% of butanediol, 4.5% of poloxamer, 3.8% of lactobacillus rhamnosus fermentation product filtrate, 1.8% of theaflavin extracting solution, 1.9% of dictyophora phalloidea skirt extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium and 71.87% of deionized water.
Compared with the embodiment 3, the comparative example deletes the carbonized polyamine nano particle solution and supplements deionized water with corresponding mass parts.
Efficacy test 2
The efficacy experiment still takes candida albicans as an experimental object, and bacteriostasis experiments are respectively carried out on the compositions related to examples 1-4 and comparative examples 1-5.
Inoculation of test bacteria by dipping with sterile cotton swab at 5 × 10 concentration5～5×106cfu/ml Candida albicans suspension was spread evenly on the surface of nutrient agar medium plate 3 times, the plate was rotated 60 times for each spread, and finally cotton swabs were spread around the edge of the plate for one week. The plate was covered and dried at room temperature for 5 min.
Pasting and placing a composition sample: each test is stuck with 1 infectious bacterium plate, and each plate is stuck with 4 test sample plates and 1 negative control sample plate, and the number of the negative control sample plates is 5. When the patch is placed, a sterile forceps sampling piece is used for being placed on the surface of the flat plate. The distance between the centers of the various pieces is more than 25mm, and the distance between the centers of the various pieces and the periphery of the flat plate is more than 15 mm. After the surface is attached, the sample wafer is lightly pressed by using sterile forceps to be tightly attached to the surface of the flat plate, the flat dish is covered, the flat plate is placed in an incubator at 37 ℃, the result is observed after the culture is carried out for 16-18 h, the diameter of the antibacterial ring is measured by using a vernier caliper and recorded, and the table of the killing effect of the composition on the candida albicans shown in the table 2 is obtained.
Table 2: candida albicans killing effect table
The following conclusions can be drawn from the above experiments: comparative example 1 no lactobacillus rhamnosus fermentation product filtrate, theaflavin extract, dictyophora indusiata skirt extract and polyamine carbide nanoparticle solution were added; in comparative examples 2-4, one of lactobacillus rhamnosus fermentation product filtrate, theaflavin extract, dictyophora indusiata skirt extract and polyamine carbide nanoparticle solution is not added; in examples 1 to 4, a lactobacillus rhamnosus fermentation product filtrate, a theaflavin extract, a dictyophora phalloidea shiitake extract, and a polyamine carbide nanoparticle solution were added. The experimental results show that under different action times, the sterilization rate of the comparative example 1 is less than that of the comparative examples 2-5 is less than that of the examples 1-4, and the addition of the lactobacillus rhamnosus fermentation product filtrate, the theaflavin extracting solution, the dictyophora indusiata skirt extracting solution and the carbonized polyamine nano particle solution can improve the antibacterial effect of the composition.
In addition, the sterilization rate of the composition according to example 3 was higher than the sterilization rate of the compositions according to examples 1, 2 and 4, and it was confirmed that the ratio of the components in the composition of example 3 was the optimum ratio.
Efficacy test 3
In the efficacy experiment, after 980 test subjects used the female private gel microecological preparation composition of example 3 for 30 days, the self-evaluation of the degree of each index related to the deep-layer vulval dark melanin precipitation and lightening effect was performed, and the self-evaluation results were as follows:
and (3) the pigmentation of the pudendum labia is reduced and improved: 85.7% and 89.2% of the subjects had moderate and significant improvement, respectively;
pudendal vaginal dryness improvement: moderate improvement and obvious improvement were found in 76.5% and 79.1% of subjects, respectively;
pudendum vaginal pruritus improvement: 75.7% and 78.5% of the subjects had moderate and significant improvement, respectively;
improving the peculiar smell of the pudendum and the vagina: moderate improvement and obvious improvement were found in 87.6% and 92.2% of subjects, respectively;
the product has different degrees of remarkable effects on pudendum pigmentation reduction, pudendum vaginal dryness, pudendum vaginal pruritus and vaginal odor.
The present invention has been described in detail with reference to the embodiments, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (10)
1. A female private gel microecological preparation composition is characterized in that: the paint comprises the following components in percentage by mass: 3.0-5.0% of glycerol, 5.0-10.0% of butanediol, 3.0-5.0% of poloxamer, 2.0-4.0% of lactobacillus rhamnosus fermentation product filtrate, 1.0-2.0% of theaflavin extracting solution, 1.0-2.0% of dictyophora fungus skirt extracting solution, 0.3-0.8% of lactic acid, 0.2-0.4% of allantoin, 0.1-0.2% of EDTA disodium, 0.0001-0.1% of carbonized polyamine nano particle solution and 84.3999-70.5% of deionized water.
2. The female intimate gel microecological formulation composition of claim 1, wherein: the total mass percentage of the components of the lactobacillus rhamnosus fermentation product filtrate, the theaflavin extract, the dictyophora phalloidea skirt extract and the carbonized polyamine nano particle solution is 7.6%.
3. The female intimate gel microecological formulation composition of claim 1, wherein: the weight percentage of each component is as follows: 5.0% of glycerol, 10.0% of butanediol, 4.5% of poloxamer, 3.8% of lactobacillus rhamnosus fermentation product filtrate, 1.8% of theaflavin extracting solution, 1.9% of dictyophora phalloidea sheel extracting solution, 0.6% of lactic acid, 0.35% of allantoin, 0.18% of EDTA disodium, 0.1% of carbonized polyamine nano particle solution and 71.77% of deionized water.
4. A method of preparing the female intimate gel microecological formulation composition of claim 1, wherein: which comprises the following steps:
1) mixing glycerol, butanediol, allantoin, EDTA disodium and deionized water, heating and stirring until the mixture is completely dispersed and dissolved to form a water phase;
2) pouring poloxamer and the water phase into a vacuum homogenizing stirring pot for vacuum homogenizing stirring;
3) sequentially adding lactobacillus rhamnosus fermentation product filtrate, dictyophora indusiata skirt extracting solution, theaflavin extracting solution and carbonized polyamine nano particle solution, and continuing to carry out vacuum homogenization and stirring;
4) adding lactic acid to adjust pH value, and pressure filtering to obtain the female private gel microecological preparation composition.
5. The method for preparing a female intimate gel microecological formulation composition of claim 4, wherein: the theaflavin extract in the step 3) is extracted from dried and sieved Sterraya black tea powder, and the extraction steps are as follows: putting black tea powder into a reaction kettle, putting the reaction kettle into a counter-current ultrasonic extractor, adding denatured ethanol-water solution for extraction, and filtering until no impurity precipitates after the extraction is finished.
6. The method for preparing a female intimate gel microecological formulation composition of claim 5, wherein: the concentration of the denatured ethanol-water solution is 62 percent; in the extraction process of the theaflavin extract, the ultrasonic power is 600W, the stirring speed is 600rpm, the temperature is controlled at 65 ℃, and the extraction time is 40 mins.
7. The method for preparing a female intimate gel microecological formulation composition of claim 4, wherein: the dictyophora indusiata skirt extracting solution in the step 3) is extracted from the dictyophora indusiata skirt powder which is dried, crushed and sieved, and the extracting steps are as follows: putting the dictyophora fungus skirt powder into a reaction kettle, putting the reaction kettle into a counter-current ultrasonic extractor, adding a modified ethanol-water solution for extraction, and filtering until no impurity precipitate is generated after the extraction is finished.
8. The method for preparing a female intimate gel microecological formulation composition of claim 7, wherein: the concentration of the denatured ethanol-water solution is 60 percent; in the extraction process of the dictyophora phalloidea skirt extract, the ultrasonic power is 700W, the stirring speed is 600rpm, the temperature is controlled at 55 ℃, and the extraction time is 50 mins.
9. The method for preparing a female intimate gel microecological formulation composition of claim 4, wherein: in the step 2), the specific steps of homogenizing and stirring include:
2.1) pouring the poloxamer and the water phase into a vacuum homogenizing and stirring pot, and then starting a homogenizing and stirring blade at the stirring speed of 600rpm and the homogenizing speed of 2000 rpm;
2.2) vacuumizing to 0.04Mpa, and continuing homogenizing and stirring for 15Mins under the vacuum state;
2.3) stirring in vacuum and cooling to 40 ℃;
the specific steps of the step 3) comprise:
3.1) after the vacuum pressure is released, sequentially adding lactobacillus rhamnosus fermentation product filtrate, dictyophora phalloidea skirt extracting solution, theaflavin extracting solution and polyamine carbide nano particle solution;
3.2) vacuumizing again, and homogenizing and stirring under the vacuum condition, wherein the stirring speed is 600rpm, the homogenizing speed is 2000rpm, and the homogenizing and stirring time is 10 Mins;
3.3) under the vacuum condition, continuously stirring and cooling to 30 ℃.
10. The method for preparing a female intimate gel microecological formulation composition of claim 1, wherein: heating to 80 ℃ in the step 1); and in the step 4), the pH value is adjusted to 4.0-4.5, and a stainless steel filter screen of 80 meshes is used for pressure filtration.
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