CN102090620A - Dictyophora indusiata fruiting body extract with antibacterial activity and uses thereof - Google Patents
Dictyophora indusiata fruiting body extract with antibacterial activity and uses thereof Download PDFInfo
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- CN102090620A CN102090620A CN2010105781964A CN201010578196A CN102090620A CN 102090620 A CN102090620 A CN 102090620A CN 2010105781964 A CN2010105781964 A CN 2010105781964A CN 201010578196 A CN201010578196 A CN 201010578196A CN 102090620 A CN102090620 A CN 102090620A
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- dictyophora phalloidea
- filter paper
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- fructification
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Abstract
The invention belongs to the field of edible fungus, and specially relates to a dictyophora indusiata fruiting body extract with antibacterial activity and uses thereof. The extract is faint yellow, yellow oily liquid in appearance, dictyophora indusiata is crushed and sieved and then extracted by a Soxhlet extractor with petroleum ether, ether, ethyl acetate, anhydrous ethanol and methanol respectively in sequence, the extracting solution is respectively concentrated at reduced pressure until dried so as to obtain various solvent extracts, and the test for antibacterial activity is performed; the solvent extracts has superior antibacterial effect for common pathogenic microorganism and putrescence microorganisms.
Description
Technical field
The present invention relates to have the dictyophora phalloidea fruiting body extract and the application thereof of bacteriostatic activity.Belong to the edible fungus field.
Background technology
Dictyophora phalloidea belongs to Gasteromycete, Phallaceae, and dictyophora phalloidea belongs to, and has another name called bamboo fungus, bamboo ginseng.Dictyophora phalloidea is nutritious, aromatic flavour, and the flavour deliciousness have the good reputation of " fungi queen ", is the delicious food in the feast, and it is nutritious, and is tender and crisp tasty and refreshing, classifies " imperial palace tribute " in history as.It is rich in 21 seed amino acids, is high protein, low-fat nutriment.
Dictyophora phalloidea is as a kind of large-scale edible fungi, except effective in cure at aspects such as antitumor, anti-inflammatory, immune enhancing, reducing blood lipid, anti-AIDS, good effect is also arranged aspect food antiseptic, there are some researches show that dictyophora phalloidea fructification water extract possesses good inhibition bacterial action.
Summary of the invention
The purpose of this invention is to provide dictyophora phalloidea fructification fat-soluble extract and application thereof with bacteriostatic activity.
The used dictyophora phalloidea of the present invention is longuette dictyophora phalloidea fructification (purchasing in the Changning County, Sichuan Province).
Technical scheme of the present invention is: with dry dictyophora phalloidea fructification, powder essence, 80 orders sieve, extract with apparatus,Soxhlet's with benzinum, ether, ethyl acetate, absolute ethyl alcohol, methyl alcohol then, wherein dictyophora phalloidea (g) is 1 with the ratio of organic solvent (mL): 8-10, extracted 2-6 hour under the boiling temperature of each solvent, collect extract, filter, decompression (being lower than 40 ℃) is concentrated into dried, obtains different fruiting body extracts.
Different extract of the present invention all has the effect of the growth of microorganism of inhibition, and antimicrobial spectrum is wide, therefore can be used as a kind of effect of microorganism antagonist, is being with a wide range of applications aspect food fresh keeping and the new drug development.
The specific embodiment
Following example will specify method of operating of the present invention, but can not be as limitation of the invention.
Embodiment 1: with dry dictyophora phalloidea fructification, powder essence, 80 orders sieve, extract with apparatus,Soxhlet's with benzinum, ether, ethyl acetate, absolute ethyl alcohol, methyl alcohol then, wherein dictyophora phalloidea (weight) is 1: 8 with the ratio of organic solvent (volume), extracts 6 hours under the boiling temperature of each solvent, collect extract, filter, decompression (being lower than 40 ℃) is concentrated into dried, obtains different fruiting body extract ZS-A, ZS-B, ZS-C, ZS-D, ZS-E.
Embodiment 2: with dry dictyophora phalloidea fructification, powder essence, 80 orders sieve, extract with apparatus,Soxhlet's with benzinum, ether, ethyl acetate, absolute ethyl alcohol, methyl alcohol then, wherein dictyophora phalloidea (weight) is 1: 10 with the ratio of organic solvent (volume), extracts 5 hours under the boiling temperature of each solvent, collect extract, filter, decompression (being lower than 40 ℃) is concentrated into dried, obtains different fruiting body extract ZS-A, ZS-B, ZS-C, ZS-D, ZS-E.
Above-mentioned two resulting ZS-A of embodiment, ZS-B, ZS-C, ZS-D and five kinds of liposoluble constituents of ZS-E, its productive rate is respectively 0.65%, 0.77%, 0.71%, 0.83%, 0.90%.
Embodiment 3: bacteriostatic experiment
Be poured into nutrient agar in the plate and shake up, thickness is as the criterion with plate 1/2 degree of depth.
(adjusting bacterial concentration is 10 to treat to solidify back adding 0.1mL bacteria suspension by nutrient agar
6-10
7CFU/mL), with the coating of sterilization spreading rod evenly, leave standstill a moment, in plate, post then and soak good filter paper, Fen Si district, be respectively blank filter paper and extract filter paper, plate back side line record, the culture dish that posts places constant incubator to cultivate, and cultivates 18-24h for bacterium and 35-37 ℃, mould, yeast 25-30 ℃ cultivation 44-48h measure antibacterial circle diameter (seeing Table).
Table one fungistatic effect relatively
Claims (4)
1. dictyophora phalloidea fructification fat-soluble extract with bacteriostatic activity, it is characterized in that outward appearance is faint yellow, yellow oily liquid, be that longuette dictyophora phalloidea fructification is through powder essence, sieve, extract through apparatus,Soxhlet's with benzinum, ether, ethyl acetate, absolute ethyl alcohol, methyl alcohol successively respectively, extract is evaporated to dried respectively, obtains each solvent extractable matter, and carrying out bacteriostatic activity test, each extract causes a disease, causes rotten microorganism the good restraining effect is arranged common.
2. the preparation method of the described dictyophora phalloidea fructification of claim 1 fat-soluble extract: it is characterized in that the dictyophora phalloidea fructification is through powder essence, 80 orders sieve, and carry out Soxhlet with co-content not then and extract backflow 2-6h, extract filters through filter paper, is lower than 40 ℃ and is evaporated to organic solvent-free.
3. the described test filter paper of claim 1 preparation method: No. 1 qualitative filter paper of Xinhua's filter paper is broken into the sequin that diameter is 6mm with card punch, with 121 ℃ of moist heat sterilization 30min in the culture dish, the filter paper of will sterilizing immerses in the dictyophora phalloidea fat-soluble extract solution, soaks 24h..
4. the described test of claim 1 microorganism comprises: staphylococcus aureus, Escherichia coli, Salmonella typhi, bacillus subtilis, salmonella, brewer's yeast, head mold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105781964A CN102090620A (en) | 2010-12-08 | 2010-12-08 | Dictyophora indusiata fruiting body extract with antibacterial activity and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105781964A CN102090620A (en) | 2010-12-08 | 2010-12-08 | Dictyophora indusiata fruiting body extract with antibacterial activity and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102090620A true CN102090620A (en) | 2011-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105781964A Pending CN102090620A (en) | 2010-12-08 | 2010-12-08 | Dictyophora indusiata fruiting body extract with antibacterial activity and uses thereof |
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| CN (1) | CN102090620A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104068449A (en) * | 2014-06-17 | 2014-10-01 | 福建农林大学 | Separation and purification method for dictyophora indusia fisscher antibacterial active monomer |
| CN105767840A (en) * | 2016-04-13 | 2016-07-20 | 浙江中医药大学 | Room-temperature food preservation sheet and preparing method thereof |
| CN109045077A (en) * | 2018-08-30 | 2018-12-21 | 青海大学 | The anticancer usage of Heterostemonous Biebersteinia fat-soluble extract |
| CN111494306A (en) * | 2020-05-29 | 2020-08-07 | 杭州百芮生物科技有限公司 | Female private gel microecological preparation composition and preparation method thereof |
| CN112023110A (en) * | 2020-08-06 | 2020-12-04 | 西安交通大学 | Active antibacterial dressing based on bamboo fungus egg extract |
-
2010
- 2010-12-08 CN CN2010105781964A patent/CN102090620A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 王家堂: "竹荪多糖的结构表征、链构象及形貌研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
| 谭敬军等: "竹荪抑菌作用研究", 《食品科学》 * |
| 谭敬军等: "竹荪抑菌特性研究", 《食品科学》 * |
| 谭敬军等: "竹荪防腐作用初探", 《湖南农业大学学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104068449A (en) * | 2014-06-17 | 2014-10-01 | 福建农林大学 | Separation and purification method for dictyophora indusia fisscher antibacterial active monomer |
| CN104068449B (en) * | 2014-06-17 | 2016-08-24 | 福建农林大学 | A kind of isolation and purification method of Cultivation of Dictyophora bacteriostatic activity monomer |
| CN105767840A (en) * | 2016-04-13 | 2016-07-20 | 浙江中医药大学 | Room-temperature food preservation sheet and preparing method thereof |
| CN109045077A (en) * | 2018-08-30 | 2018-12-21 | 青海大学 | The anticancer usage of Heterostemonous Biebersteinia fat-soluble extract |
| CN111494306A (en) * | 2020-05-29 | 2020-08-07 | 杭州百芮生物科技有限公司 | Female private gel microecological preparation composition and preparation method thereof |
| CN112023110A (en) * | 2020-08-06 | 2020-12-04 | 西安交通大学 | Active antibacterial dressing based on bamboo fungus egg extract |
| CN112023110B (en) * | 2020-08-06 | 2021-11-19 | 西安交通大学 | Active antibacterial dressing based on bamboo fungus egg extract |
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Application publication date: 20110615 |