CN111474335A - Grain mycotoxin on-line detection method and device based on immunosensor - Google Patents
Grain mycotoxin on-line detection method and device based on immunosensor Download PDFInfo
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- CN111474335A CN111474335A CN202010161240.5A CN202010161240A CN111474335A CN 111474335 A CN111474335 A CN 111474335A CN 202010161240 A CN202010161240 A CN 202010161240A CN 111474335 A CN111474335 A CN 111474335A
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- 231100000678 Mycotoxin Toxicity 0.000 title claims abstract description 41
- 239000002636 mycotoxin Substances 0.000 title claims abstract description 41
- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 238000005070 sampling Methods 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000002955 isolation Methods 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000005096 rolling process Methods 0.000 claims description 6
- 230000007613 environmental effect Effects 0.000 abstract description 2
- IDGRYIRJIFKTAN-UHFFFAOYSA-N 3-acetyldeoxynivalenol Natural products CC(=O)OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 IDGRYIRJIFKTAN-UHFFFAOYSA-N 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- ITCSWEBPTQLQKN-UHFFFAOYSA-N Nivalenol Natural products CC1=CC2OC3C(O)C(O)C(C2(CO)CC1=O)C34CO4 ITCSWEBPTQLQKN-UHFFFAOYSA-N 0.000 description 3
- UKOTXHQERFPCBU-YQPARWETSA-N Nivalenol Chemical compound C([C@]12[C@@]3([C@H](O)[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 UKOTXHQERFPCBU-YQPARWETSA-N 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- IDGRYIRJIFKTAN-HTJQZXIKSA-N 15-acetyldeoxynivalenol Chemical compound C([C@@]12[C@]3(C)C[C@@H](O)[C@H]1O[C@@H]1C=C(C)C(=O)[C@@H](O)[C@@]13COC(=O)C)O2 IDGRYIRJIFKTAN-HTJQZXIKSA-N 0.000 description 2
- ADFIQZBYNGPCGY-HTJQZXIKSA-N 3-acetyldeoxynivalenol Chemical compound C([C@]12[C@]3(C)C[C@H]([C@H]2O[C@H]2[C@@]3([C@H](O)C(=O)C(C)=C2)CO)OC(=O)C)O1 ADFIQZBYNGPCGY-HTJQZXIKSA-N 0.000 description 2
- ADFIQZBYNGPCGY-UHFFFAOYSA-N Acetyldeoxynivalenol Natural products C1=C(C)C(=O)C(O)C2(CO)C1OC1C(OC(=O)C)CC2(C)C21CO2 ADFIQZBYNGPCGY-UHFFFAOYSA-N 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 description 2
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 description 2
- BXFOFFBJRFZBQZ-QYWOHJEZSA-N T-2 toxin Chemical compound C([C@@]12[C@]3(C)[C@H](OC(C)=O)[C@@H](O)[C@H]1O[C@H]1[C@]3(COC(C)=O)C[C@@H](C(=C1)C)OC(=O)CC(C)C)O2 BXFOFFBJRFZBQZ-QYWOHJEZSA-N 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000002115 aflatoxin B1 Substances 0.000 description 1
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 1
- 229930020125 aflatoxin-B1 Natural products 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- LINOMUASTDIRTM-QGRHZQQGSA-N deoxynivalenol Chemical compound C([C@@]12[C@@]3(C[C@@H](O)[C@H]1O[C@@H]1C=C(C([C@@H](O)[C@@]13CO)=O)C)C)O2 LINOMUASTDIRTM-QGRHZQQGSA-N 0.000 description 1
- 229930002954 deoxynivalenol Natural products 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- UVBUBMSSQKOIBE-DSLOAKGESA-N fumonisin B1 Chemical compound OC(=O)C[C@@H](C(O)=O)CC(=O)O[C@H]([C@H](C)CCCC)[C@@H](OC(=O)C[C@@H](CC(O)=O)C(O)=O)C[C@@H](C)C[C@H](O)CCCC[C@@H](O)C[C@H](O)[C@H](C)N UVBUBMSSQKOIBE-DSLOAKGESA-N 0.000 description 1
- QZIADBYRQILELJ-UHFFFAOYSA-N fumonisin B1 Natural products CCCCC(C)C(OC(=O)CC(CC(=O)O)C(=O)O)C(C)(CC(C)CC(O)CCCCC(O)CC(O)C(C)N)OC(=O)CC(CC(=O)O)C(=O)O QZIADBYRQILELJ-UHFFFAOYSA-N 0.000 description 1
- PNKLMTPXERFKEN-ZIOSACBISA-N mycotoxin ht 2 Chemical compound C([C@]12[C@]3(C)[C@H](O)[C@@H](O)[C@H]1O[C@H]1[C@]3(COC(C)=O)C[C@@H](C(=C1)C)OC(=O)CC(C)C)O2 PNKLMTPXERFKEN-ZIOSACBISA-N 0.000 description 1
- RWQKHEORZBHNRI-BMIGLBTASA-N ochratoxin A Chemical compound C([C@H](NC(=O)C1=CC(Cl)=C2C[C@H](OC(=O)C2=C1O)C)C(O)=O)C1=CC=CC=C1 RWQKHEORZBHNRI-BMIGLBTASA-N 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- LINOMUASTDIRTM-UHFFFAOYSA-N vomitoxin hydrate Natural products OCC12C(O)C(=O)C(C)=CC1OC1C(O)CC2(C)C11CO1 LINOMUASTDIRTM-UHFFFAOYSA-N 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Sampling And Sample Adjustment (AREA)
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Abstract
The invention relates to an on-line detection method and device for grain mycotoxin based on an immunosensor. The method and the device for detecting the grain mycotoxin on line based on the immunosensor comprise the following steps: s1: sampling the grain stored in the granary; s2: grinding the sampled sample to prepare a liquid; s3: the solution after the solution preparation is placed into an immunosensor for detection, the immunosensor outputs a detection result, and the detection result is transmitted to a background computer; s4: the background computer controls the isolation mechanism to isolate the stored grains; the method and the device for detecting the grain mycotoxin on line based on the immunosensor have high practicability, are controlled by a computer, are convenient to ensure the environmental safety of more granaries, are convenient for people to use and are convenient for popularization in the industry.
Description
Technical Field
The invention belongs to the technical field of grain detection, and particularly relates to an on-line detection method and device for grain mycotoxin based on an immunosensor.
Background
There are many edible mycotoxins, such as aflatoxin B1, B2, G1, G2, deoxynivalenol (NIV) and its derivatives 3-acetyl deoxynivalenol (3-Ac-DON), 15-acetyl deoxynivalenol (15-Ac-DON), Zearalenone (ZEN), fumonisin B1, B2, B3, ochratoxin A (OTA), T-2 toxin and HT-2 toxin, Nivalenol (NIV), etc.; the immunosensor is a biosensor, develops rapidly in recent years, and is gradually applied to the fields of food, industry, environmental detection, clinical medicine and the like. Immunosensors are an emerging biosensor for which high specificity, sensitivity and stability of identified substances are favored, and their emergence has greatly changed the conventional immunoassays. The method integrates the traditional immunoassay and biosensing technologies into a whole, integrates a plurality of advantages of the immunoassay and the biosensing technologies into a whole, reduces analysis time, improves sensitivity and test precision, simplifies the determination process, is easy to realize automation and has wide application prospect; the immunosensor is applied to regular detection of grain storage of the granary, so that the pollution degree of the granary can be reduced as much as possible.
Disclosure of Invention
The invention aims to solve the problems and provide a food mycotoxin on-line detection method and device based on an immunosensor, which are simple in structure and reasonable in design.
The invention realizes the purpose through the following technical scheme:
an on-line detection method of food mycotoxin based on an immunosensor comprises the following steps of S1: sampling the grain stored in the granary; s2: grinding the sampled sample to prepare a liquid; s3: the solution after the solution preparation is placed into an immunosensor for detection, the immunosensor outputs a detection result, and the detection result is transmitted to a background computer; s4: the background computer controls the isolation mechanism to isolate the stored grains.
As a further optimization scheme of the present invention, S1: and (4) extracting grains at the bottom, the middle part and the top of the granary for sampling, and respectively storing the grains in sampling containers.
As a further optimization scheme of the present invention, S1: and (4) extracting the grains on the side wall part of the granary for sampling.
As a further optimization scheme of the present invention, S2: grinding the sampled grains at room temperature, adding into a container with the extracting solution therein, and standing for 0.5-3 h.
As a further optimization scheme of the present invention, S5: when the grain samples at the top, the middle and the bottom all show mycotoxin signals, isolating the whole granary; when the grain samples only at the top and the middle part show mycotoxin signals, sampling and detecting the grain samples at the position between the middle part and the bottom part, if the part has no mycotoxin signal, isolating the grain from the top to the position detected at the time, and if the part has the mycotoxin signal, isolating the grain from the top to the middle part; and when the grain sample only on the top shows the mycotoxin signal, sampling and detecting the grain at the position between the top and the middle part, if the mycotoxin signal of the part exists, isolating the grain from the top to the position detected at the time, and if the mycotoxin signal exists in the part, isolating the grain from the top to the middle part.
The utility model provides a grain mycotoxin on-line measuring device based on immunosensor, includes sampling mechanism, rolling mechanism, joins in marriage liquid mechanism, detection mechanism and isolation mechanism, sampling mechanism includes the grain sample ware, rolling mechanism includes the attrition mill, join in marriage liquid mechanism including shaking the machine, detection mechanism includes immunosensor.
The invention has the beneficial effects that: the device and the method can regularly sample and detect the grains in the granary, carry out multi-region sampling on the grains in the granary, find the mycotoxin after sampling, carry out refined sampling detection again, and are convenient for isolating the polluted grain quantity in the granary in a targeted manner.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
Example 1
As shown in figure 1, the food mycotoxin on-line detection method based on the immunosensor comprises the following steps: s1: sampling the grains stored in the granary, wherein the sampling device only adopts a grain sampler with enough length to sample the grains at the bottom, the middle and the top of the granary and respectively store the grains in a sampling container, the partial sampling is performed from the middle as far as possible, the number of samples can be increased if necessary, and the grains on the side wall part of the granary are extracted for sampling; s2: grinding the sampled sample to prepare the liquid, grinding by using a common grinding machine at room temperature (25 ℃) as far as possible, grinding the sampled grain at room temperature, adding the ground grain into a container with the extracting solution, standing for 0.5-3 h, and optimally standing for 2.5 h; s3: the solution after the solution preparation is placed into an immunosensor for detection, the immunosensor outputs detection results, the detection results are divided into two types, namely, existence confirmation and nonexistence confirmation, and the two groups of detection results are transmitted to a background computer; s4: the background computer controls the isolation mechanism to isolate the stored grains; s5: when the grain samples at the top, the middle and the bottom all show mycotoxin signals, isolating the whole granary; when the grain samples only at the top and the middle part show mycotoxin signals, sampling and detecting the grain samples at the position between the middle part and the bottom part, if the part has no mycotoxin signal, isolating the grain from the top to the position detected at the time, and if the part has the mycotoxin signal, isolating the grain from the top to the middle part; and when the grain sample only on the top shows the mycotoxin signal, sampling and detecting the grain at the position between the top and the middle part, if the mycotoxin signal of the part exists, isolating the grain from the top to the position detected at the time, and if the mycotoxin signal exists in the part, isolating the grain from the top to the middle part.
The utility model provides a grain mycotoxin on-line measuring device based on immunosensor, includes sampling mechanism, rolling mechanism, joins in marriage liquid mechanism, detection mechanism and isolation mechanism, sampling mechanism includes the grain sample ware, rolling mechanism includes the attrition mill, join in marriage liquid mechanism including shaking the machine, detection mechanism includes immunosensor.
The food mycotoxin on-line detection method and device based on the immunosensor are used.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (6)
1. An on-line detection method for grain mycotoxin based on an immunosensor is characterized by comprising the following steps: s1: sampling the grain stored in the granary; s2: grinding the sampled sample to prepare a liquid; s3: the solution after the solution preparation is placed into an immunosensor for detection, the immunosensor outputs a detection result, and the detection result is transmitted to a background computer; s4: the background computer controls the isolation mechanism to isolate the stored grains.
2. The on-line detection method of food mycotoxin based on immunosensor according to claim 1, wherein the method comprises the following steps: s1: and (4) extracting grains at the bottom, the middle part and the top of the granary for sampling, and respectively storing the grains in sampling containers.
3. The on-line detection method and device of food mycotoxin based on immunosensor of claim 2, wherein the method comprises the following steps: s1: and (4) extracting the grains on the side wall part of the granary for sampling.
4. The on-line detection method and device of food mycotoxin based on immunosensor of claim 3, wherein the method comprises the following steps: s2: grinding the sampled grains at room temperature, adding into a container with the extracting solution therein, and standing for 0.5-3 h.
5. The on-line detection method and device of food mycotoxin based on immunosensor of claim 4, wherein the method comprises the following steps: s5: when the grain samples at the top, the middle and the bottom all show mycotoxin signals, isolating the whole granary; when the grain samples only at the top and the middle part show mycotoxin signals, sampling and detecting the grain samples at the position between the middle part and the bottom part, if the part has no mycotoxin signal, isolating the grain from the top to the position detected at the time, and if the part has the mycotoxin signal, isolating the grain from the top to the middle part; and when the grain sample only on the top shows the mycotoxin signal, sampling and detecting the grain at the position between the top and the middle part, if the mycotoxin signal of the part exists, isolating the grain from the top to the position detected at the time, and if the mycotoxin signal exists in the part, isolating the grain from the top to the middle part.
6. The on-line detection device for the food mycotoxin based on the immunosensor according to claim 1, wherein the device comprises: including sampling mechanism, rolling mechanism, join in marriage liquid mechanism, detection mechanism and isolation mechanism, sampling mechanism includes the grain sample ware, rolling mechanism includes the attrition mill, it includes the vibration machine to join in marriage liquid mechanism, detection mechanism includes immunosensor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010161240.5A CN111474335A (en) | 2020-03-10 | 2020-03-10 | Grain mycotoxin on-line detection method and device based on immunosensor |
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| CN202010161240.5A CN111474335A (en) | 2020-03-10 | 2020-03-10 | Grain mycotoxin on-line detection method and device based on immunosensor |
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