CN110595933A - Method for rapidly detecting storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology - Google Patents
Method for rapidly detecting storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology Download PDFInfo
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Abstract
The invention discloses a method for rapidly detecting the storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies. The method comprises the steps of obtaining a moisture absorption kinetic curve and a moisture absorption isotherm of the sea cucumber protein peptide powder through a DVS system, separating volatile substances through a GC-IMS, entering an IMS unit after the separation is finished, measuring twice, and successfully constructing the flavor fingerprint of the sea cucumber protein peptide powder. The method can distinguish the sea cucumber protein peptide powder packaged and stored under different conditions, can be applied to the quality classification and shelf life research of the sea cucumber protein peptide powder, has important significance, and has the characteristics of small raw material consumption, simple operation, high detection speed, high accuracy and the like.
Description
Technical Field
The invention relates to the technical field of flavor chemical analysis, in particular to a method for rapidly detecting the storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
Background
The compendium of materia Medica is recorded in Shi Yi (supplement to compendium of materia Medica): sea cucumber (sea cucumber), the taste is sweet and salty, tonify the kidney, benefit the essence and marrow, take urine, strengthen yang and cure flaccidity, its nature is tonified, it is sufficient to enemy ginseng, so the name sea cucumber, sea cucumber is regarded as one of eight precious sea products in the world, not only precious food, especially the top-grade famous medicinal materials, there are about 140 kinds of sea cucumbers in our country, edible sea cucumber about 20 kinds among them, sea cucumber contains a large amount of protein and many kinds of trace elements beneficial to human body, it is a high-quality nutritious food without cholesterol, with the continuous deepening of research and popularization of peptide knowledge, the peptide product will be known and accepted by more producers and consumers, sea cucumber protein peptide makes the good delicacy treasure on traditional wine mat become people's daily life nutritive value higher, eat more convenient high-quality health food; however, in the processing and storage process of the sea cucumber protein peptide powder, the phenomena of moisture absorption, stickiness, caking and the like gradually deteriorate, the storage stability of the sea cucumber protein peptide powder is greatly reduced, the application of the sea cucumber protein peptide powder is seriously influenced, and after the sea cucumber protein peptide powder absorbs moisture, the sea cucumber protein peptide powder is particularly easy to denature and deteriorate along with the increase of storage time, and a series of adverse effects such as polypeptide texture change, nutrient loss, function loss and the like are caused along with the generation of volatile substances, so that the quality and the stability of the product are seriously influenced, and researches show that the volatile components of the sea cucumber protein peptide powder in the sterile moist and sterile dry storage process are mainly 2,3-butandione (2, 3-butanedione), Dimethyl disulfide (2, 5-dimethylpyrazine), 1-Pentanol (n-Pentanol), butanal acid (butyric acid), 3-methyl-and Ethanol, Benzaldehyde and the like, wherein the content of 2, 3-butanedione and dimethyl disulfide is high at 0 day, the other time is basically zero, n-amyl alcohol and pentanal are greatly generated at 1 day, the concentration is gradually reduced, meanwhile, a small part of volatile organic matters such as butyric acid, 3-methyl-and Ethanol and the like are gradually increased along with the prolonging of the storage time, the storage time has great influence on the flavor of the sea cucumber protein peptide powder in aseptic moist packaging, and the concentration and the variety of the volatile organic matters are greatly increased after the sample is stored for 1 day; then, the total concentration of volatile organic compounds is reduced along with the prolonging of the storage time, meanwhile, a small part of volatile organic compounds such as butyric acid, 3-methyl-and the like are gradually increased along with the prolonging of the storage time, under the condition of aseptic dry packaging, the concentration of a large amount of volatile organic compounds is gradually reduced along with the prolonging of the storage time, and the concentration of a small amount of substances is gradually increased along with the prolonging of the storage time.
Disclosure of Invention
The invention aims to provide a method for rapidly detecting the storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
The technical scheme adopted by the invention for realizing the purpose is as follows: a method for rapidly detecting the storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology is characterized by comprising the following steps:
A. taking the sea cucumber protein peptide powder as a raw material, and constructing a characteristic fingerprint spectrum of the volatile substances of the aseptically packaged sea cucumber protein peptide powder by using a DVS technology and a GC-IMS technology;
B. the DVS technology in the step A is that a group of 0.02-0.03g of sea cucumber protein peptide powder is put on a sample disc of a dynamic moisture adsorption instrument, high-purity nitrogen with constant flow is introduced into the dynamic moisture adsorption instrument to balance until the experimental temperature is 25 ℃, the relative humidity is 0-5% RH, the data of sample quality, relative humidity and the like are automatically recorded once every 50-60s, when the change speed of the sample quality is continuously less than 0.001%/min-0.003%/min twice or the balance time exceeds 350min-360min, the sample is considered to reach the moisture absorption balance and is forced to be increased to the next-stage relative humidity, the 0% RH-90% RH in a sample chamber changes in a 10% humidity gradient, the 90% RH-95% RH changes in a 5% gradient, the relative humidity rises from the minimum value 0% to the maximum value 95% and then falls to the minimum value 0%, completing two cycles to obtain a moisture absorption kinetic curve and a moisture absorption isotherm of the sea cucumber protein peptide powder;
C. and the GC-IMS technology in the step A comprises the steps of sequentially placing 2 groups of sea cucumber protein peptide powder which are stored in a dryer and reach the experimental time point into a 20mL headspace sample injection bottle, incubating under a certain condition, injecting the sample into an FS-SE-54-CB capillary tube, taking nitrogen as carrier gas, setting the flow rate to be 2mL/min-150mL/min, setting the column temperature to be 40 ℃, and allowing gas chromatography separation components to enter an IMS unit at the temperature of 40-50 ℃ for determination.
The protein content of the sea cucumber protein peptide powder in the step A is 50-70%.
In the DVS technology in the step B, the optimal experimental conditions of aseptic moisture are 25 ℃ and 70-80% RH; the optimal experimental conditions for aseptic drying are 25 ℃ and 0-2% RH.
And the GC-IMS technology in the step C is stored in a dryer for 0, 5, 10, 15, 30 and 90 days.
In the GC-IMS technology in the step C, the mass of the 2 groups of sea cucumber protein peptide powder is 0.2-0.3g respectively.
And in the GC-IMS technology in the step C, the incubation condition is 50-52 ℃ and 30-40 min.
In the GC-IMS technology in the step C, the sample injection amount is 400-.
The invention relates to a method for rapidly detecting the storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies, which combines DVS and GC-IMS technologies, is high in efficiency, rapidness, simple in operation and high in accuracy and reliability, and is used for qualitative and semi-quantitative analysis of compounds; the flavor fingerprint spectrum of the sea cucumber protein peptide powder is constructed by two technologies of DVS and GC-IMS, can be used for identification and discrimination and grade classification of the aseptically packaged sea cucumber protein peptide powder, and has important significance for quality control of the sea cucumber protein peptide powder.
According to the technical method, the sea cucumber protein peptide powder packaged and stored under different conditions can be distinguished, 3-4g of the sea cucumber protein peptide powder is used as a raw material, the using amount is small, the method is an effective method for saving the raw material, the fingerprint of the volatile substance of the aseptically packaged sea cucumber protein peptide powder is provided, and compared with the fingerprint of a sample to be detected, the quick identification of the sea cucumber protein peptide powder sample can be realized, and the method can be applied to the quality grading and shelf life research of the sea cucumber protein peptide powder and has important significance.
Drawings
FIG. 1 is a moisture absorption kinetic curve of the sea cucumber protein peptide powder of the method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
FIG. 2 is a moisture absorption isotherm of the sea cucumber protein peptide powder of the method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
FIG. 3 is a characteristic fingerprint of the sea cucumber protein peptide powder stored in the aseptic wet package for 0, 5 and 10 days, which is a method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
FIG. 4 is a characteristic fingerprint of the sea cucumber protein peptide powder stored in the aseptic wet package for 15, 30 and 90 days, which is a method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
FIG. 5 is a characteristic fingerprint of the sea cucumber protein peptide powder stored in the aseptic dry package for 0, 5 and 10 days, which is a method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
FIG. 6 is a characteristic fingerprint of the sea cucumber protein peptide powder stored in the aseptic dry package for 15, 30 and 90 days, which is a method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies.
Detailed Description
The invention relates to a method for rapidly detecting the storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technologies, which takes the sea cucumber protein peptide powder as a raw material and utilizes the DVS technology and the GC-IMS technology to construct a characteristic fingerprint spectrum of volatile substances of the aseptically packaged sea cucumber protein peptide powder; the DVS technology is that a group of 0.02-0.03g of sea cucumber protein peptide powder is put on a sample plate of a dynamic moisture adsorption instrument, high-purity nitrogen with constant flow is introduced into the dynamic moisture adsorption instrument to balance until the experimental temperature is 25 ℃, the relative humidity is 0-5% RH, the data of sample quality, relative humidity and the like are automatically recorded once every 50-60s, when the change speed of the sample quality is continuously less than 0.001%/min-0.003%/min twice or the balance time exceeds 350min-360min, the sample is considered to reach the moisture absorption balance, the sample is forced to increase to the next-stage relative humidity, 0% RH-90% RH in a sample chamber changes with 10% humidity gradient, 90% RH-95% RH changes with 5% gradient, the relative humidity rises from the minimum value of 0% to the maximum value of 95% and then falls to the minimum value of 0%, and two cycles are completed, obtaining a moisture absorption kinetic curve and a moisture absorption isotherm of the sea cucumber protein peptide powder;
the GC-IMS technology is characterized in that 2 groups of sea cucumber protein peptide powder which are stored in a dryer and reach the experimental time point are sequentially placed in a 20mL headspace sample injection bottle, incubated under a certain condition, injected into an FS-SE-54-CB capillary tube, nitrogen is used as carrier gas, the flow is set to be 2mL/min-150mL/min, the column temperature is 40 ℃, gas chromatography separation components enter an IMS unit with the temperature of 40-50 ℃ for measurement, the protein content of the sea cucumber protein peptide powder is 50% -70%, and the optimal experimental conditions of aseptic moisture of the DVS technology are 25 ℃ and 70-80% RH; the optimal experimental conditions for aseptic drying are 25 ℃, 0-2% RH, the storage time of the GC-IMS technology in a dryer is 0, 5, 10, 15, 30 and 90 days, the mass of the GC-IMS technology and the mass of the 2 groups of sea cucumber protein peptide powder are 0.2-0.3g respectively, the incubation condition is 50-52 ℃, 30-40min, and the sample injection amount is 400 plus 600 mu L.
Example 1:
weighing a group of 0.022g of sea cucumber protein peptide powder, putting the sea cucumber protein peptide powder on a sample plate of a dynamic moisture adsorption instrument, and introducing high-purity nitrogen with constant flow into the dynamic moisture adsorption instrument to balance the sea cucumber protein peptide powder until the experimental temperature is 25 ℃ and the relative humidity is 2% RH; automatically recording data such as sample mass, relative humidity and the like once every 55s, and when the sample mass change speed is less than 0.0015%/min twice continuously or the balance time exceeds 350min, determining that the sample reaches the moisture absorption balance, and further forcibly increasing the sample to the next-stage relative humidity; changing 0RH-90% RH in a 10% humidity gradient manner, changing 90% RH-95% RH in a 5% gradient manner, increasing 0% RH to a maximum value of 95% RH and then decreasing to a minimum value of 0% RH, completing two cycles, obtaining a moisture absorption dynamics curve diagram 1 and a moisture absorption isotherm diagram 2 of the sea cucumber protein peptide powder, and determining that the sterile moisture condition which is most suitable for the experiment is 25 ℃ and 70% RH; weighing 0.25g of sea cucumber protein peptide powder stored in a 70% RH sterile package, and placing the sea cucumber protein peptide powder into a 20mL headspace sample injection bottle; automatically injecting samples after incubation for 40 minutes at 52 ℃, wherein the temperature of a sample injection needle is 85 ℃, the sample injection volume is 400 mu L, and a non-shunting mode is adopted; the gas chromatographic column is FS-SE-54-CB-0.5 capillary chromatographic column (15 m is multiplied by 0.53 mm), and the temperature of the chromatographic column is 40 ℃; the carrier gas is high-purity nitrogen with the purity of 99.99 percent, and the flow rate is set to be 2mL/min-150 mL/min; samples for each storage condition were assayed in parallel twice; calculating the Retention Index (RI) of each volatile compound by taking n-ketonesC4-C9 as an internal standard; comparing RI value and Drift Time (DT) of volatile compounds with GC-IMS database, and obtaining characteristic fingerprint of sea cucumber protein peptide powder stored in aseptic wet package for 0, 5 and 10 days by LAV (laboratory Analytical viewer) built in software; determining volatile compounds of the sterile and wet packaged sea cucumber protein peptide powder in the storage process; finally, as can be seen from fig. 3, the concentration and species of volatile organic compounds greatly increased after 1 day of storage of the samples under aseptic moisture packaging conditions.
Example 2:
weighing a group of 0.025g of sea cucumber protein peptide powder, putting the sea cucumber protein peptide powder on a sample disc of a dynamic moisture adsorption instrument, and introducing high-purity nitrogen with constant flow into the dynamic moisture adsorption instrument to balance the experiment temperature to 25 ℃ and the relative humidity to 5% RH; automatically recording data such as sample mass and relative humidity once every 52s, and when the sample mass change speed is less than 0.003%/min twice continuously or the balance time exceeds 355min, determining that the sample reaches the moisture absorption balance, and further forcibly increasing the sample to the next relative humidity; changing 0RH-90% RH in a 10% humidity gradient manner, changing 90% RH-95% RH in a 5% gradient manner, increasing 0% RH to a maximum value of 95% RH and then decreasing to a minimum value of 0% RH, completing two cycles, obtaining a moisture absorption dynamics curve diagram 1 and a moisture absorption isotherm diagram 2 of the sea cucumber protein peptide powder, and determining that the sterile moisture condition which is most suitable for the experiment is 25 ℃ and 75% RH; weighing 0.28g of sea cucumber protein peptide powder stored in a sterile package of 70-80% RH, and placing the sea cucumber protein peptide powder into a 20mL headspace sample injection bottle; automatically injecting samples after incubating for 35 minutes at 50 ℃, wherein the temperature of a sample injection needle is 85 ℃, and the sample injection volume is 550 mu L; and a non-shunting mode is adopted; the gas chromatographic column is FS-SE-54-CB-0.5 capillary chromatographic column (15 m is multiplied by 0.53 mm), and the temperature of the chromatographic column is 40 ℃; the carrier gas is high-purity nitrogen with the purity of 99.99 percent, and the flow rate is set to be 2mL/min-150 mL/min; samples for each storage condition were assayed in parallel twice; calculating the Retention Index (RI) of each volatile compound by taking n-ketonesC4-C9 as an internal standard; comparing RI value and Drift Time (DT) of volatile compounds with GC-IMS database, and obtaining characteristic fingerprint of sea cucumber protein peptide powder stored for 15, 30 and 90 days under aseptic moist packaging condition by LAV (laboratory Analytical viewer) arranged in software; determining volatile compounds of the sterile and wet packaged sea cucumber protein peptide powder in the storage process; finally, as can be seen from fig. 4, the overall concentration of volatile organics decreased with storage time under aseptic moisture packaging conditions.
Example 3:
weighing a group of 0.026g of sea cucumber protein peptide powder, placing the sea cucumber protein peptide powder on a sample disc of a dynamic moisture adsorption instrument, introducing high-purity nitrogen with constant flow into the dynamic moisture adsorption instrument to balance the experimental temperature to 25 ℃, the relative humidity to 0% RH, automatically recording data such as sample mass and relative humidity once every 58s, when the sample mass change speed is continuously less than 0.0025%/min twice or the balance time exceeds 350min, determining that the sample reaches moisture absorption balance, then forcibly increasing the moisture absorption balance to the next stage of relative humidity, changing the 0RH-90% RH in a 10% humidity gradient in a sample chamber, changing the 90% RH ~% RH in a 5% gradient, increasing the 0% RH to the maximum 95% RH and then decreasing the RH to the minimum 0% RH, completing two cycles, obtaining a moisture absorption dynamics curve diagram 1 and a moisture absorption isotherm diagram 2 of the sea cucumber protein peptide powder, obtaining the optimal experimental condition of 2% RH, weighing 0.25g of the sea cucumber protein peptide powder stored under the 2% RH aseptic packaging condition, placing the sea cucumber protein peptide powder into a top empty sample bottle, incubating the sample bottle at 30 minutes, obtaining an automatic sample introduction temperature after the sample injection curve, and obtaining a dry sample concentration of the sample by a dry sample, gradually increasing the sample concentration by a molecular weight of the dry sample after the dry sample concentration of the sample is determined by a molecular weight of the sample, and a molecular weight of a molecular weight distribution curve of a molecular weight distribution curve of a molecular weight curve of a molecular curve of.
Example 4:
weighing a group of 0.025g of sea cucumber protein peptide powder, putting the sea cucumber protein peptide powder on a sample disc of a dynamic moisture adsorption instrument, and introducing high-purity nitrogen with constant flow into the dynamic moisture adsorption instrument to balance the experiment temperature to 25 ℃ and the relative humidity to be 3% RH; automatically recording data such as sample mass, relative humidity and the like once every 1min, and when the sample mass change speed is less than 0.0025%/min for two times continuously or the balance time exceeds 355min, determining that the sample reaches the moisture absorption balance, and further forcibly increasing the sample to the next relative humidity; changing 0RH-90% RH in a 10% humidity gradient in a sample chamber, changing 90% RH-95% RH in a 5% gradient, increasing 0% RH to a maximum value of 95% RH and then decreasing to a minimum value of 0% RH, completing two cycles, obtaining a moisture absorption dynamics curve diagram 1 and a moisture absorption isotherm diagram 2 of sea cucumber protein peptide powder, determining that the most suitable sterile moisture condition of an experiment is 25 ℃, and obtaining the best experiment condition of 0% RH; weighing 0.24g of sea cucumber protein peptide powder stored under the sterile packaging condition of 0% RH, and placing the sea cucumber protein peptide powder into a 20mL headspace sample injection bottle; automatically injecting samples after incubating for 36 minutes at 51 ℃, wherein the temperature of a sample injection needle is 85 ℃, and the sample injection volume is 600 mu L; and a non-shunting mode is adopted; the gas chromatographic column is FS-SE-54-CB-0.5 capillary chromatographic column (15 m is multiplied by 0.53 mm), and the temperature of the chromatographic column is 40 ℃; the carrier gas is high-purity nitrogen with the purity of 99.99 percent, and the flow rate is set to be 2mL/min-150 mL/min; samples for each storage condition were assayed in parallel twice; calculating the Retention Index (RI) of each volatile compound by taking n-ketonesC4-C9 as an internal standard; comparing RI value and Drift Time (DT) of volatile compounds with GC-IMS database, and obtaining characteristic fingerprint of sea cucumber protein peptide powder stored for 15, 30 and 90 days under aseptic dry packaging condition by LAV (laboratory analytical viewer) built in software; determining volatile compounds of the aseptically dried and packaged sea cucumber protein peptide powder in the storage process; finally, as can be seen from fig. 6, under aseptic dry packaging conditions, the concentration of a large amount of volatile organic compounds gradually decreased with the lapse of storage time, while the concentration of a small amount of substances gradually increased with the lapse of storage time.
Claims (7)
1. A method for rapidly detecting the storage quality of aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology is characterized by comprising the following steps:
A. taking the sea cucumber protein peptide powder as a raw material, and constructing a characteristic fingerprint spectrum of the volatile substances of the aseptically packaged sea cucumber protein peptide powder by using a DVS technology and a GC-IMS technology;
B. the DVS technology in the step A is that a group of 0.02-0.03g of sea cucumber protein peptide powder is put on a sample disc of a dynamic moisture adsorption instrument, high-purity nitrogen with constant flow is introduced into the dynamic moisture adsorption instrument to balance until the experimental temperature is 25 ℃, the relative humidity is 0-5% RH, the data of sample quality, relative humidity and the like are automatically recorded once every 50-60s, when the change speed of the sample quality is continuously less than 0.001%/min-0.003%/min twice or the balance time exceeds 350min-360min, the sample is considered to reach the moisture absorption balance and is forced to be increased to the next-stage relative humidity, the 0% RH-90% RH in a sample chamber changes in a 10% humidity gradient, the 90% RH-95% RH changes in a 5% gradient, the relative humidity rises from the minimum value 0% to the maximum value 95% and then falls to the minimum value 0%, completing two cycles to obtain a moisture absorption kinetic curve and a moisture absorption isotherm of the sea cucumber protein peptide powder;
C. and the GC-IMS technology in the step A comprises the steps of sequentially placing 2 groups of sea cucumber protein peptide powder which are stored in a dryer and reach the experimental time point into a 20mL headspace sample injection bottle, incubating under a certain condition, injecting the sample into an FS-SE-54-CB capillary tube, taking nitrogen as carrier gas, setting the flow rate to be 2mL/min-150mL/min, setting the column temperature to be 40 ℃, and allowing gas chromatography separation components to enter an IMS unit at the temperature of 40-50 ℃ for determination.
2. The method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology according to claim 1, which is characterized in that: the protein content of the sea cucumber protein peptide powder in the step A is 50-70%.
3. The method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology according to claim 1, which is characterized in that: in the DVS technology in the step B, the optimal experimental conditions of aseptic moisture are 25 ℃ and 70-80% RH; the optimal experimental conditions for aseptic drying are 25 ℃ and 0-2% RH.
4. The method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology according to claim 1, which is characterized in that: and the GC-IMS technology in the step C is stored in a dryer for 0, 5, 10, 15, 30 and 90 days.
5. The method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology according to claim 1, which is characterized in that: in the GC-IMS technology in the step C, the mass of the 2 groups of sea cucumber protein peptide powder is 0.2-0.3g respectively.
6. The method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology according to claim 1, which is characterized in that: and in the GC-IMS technology in the step C, the incubation condition is 50-52 ℃ and 30-40 min.
7. The method for rapidly detecting the storage quality of the aseptically packaged sea cucumber protein peptide powder based on DVS and GC-IMS technology according to claim 1, which is characterized in that: in the GC-IMS technology in the step C, the sample injection amount is 400-.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112578042A (en) * | 2020-11-25 | 2021-03-30 | 广西壮族自治区农业科学院 | Grape postharvest storage period evaluation method based on characteristic aroma substance fingerprint spectrum |
| CN114062620A (en) * | 2021-11-04 | 2022-02-18 | 华南农业大学 | Evaluation method for stable storage of milk hypnotize peptides |
| CN114720611A (en) * | 2022-04-02 | 2022-07-08 | 云南中烟工业有限责任公司 | Method for analyzing shelf life attenuation characteristics of aroma compounds of finished cigarette paper |
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| CN114062620A (en) * | 2021-11-04 | 2022-02-18 | 华南农业大学 | Evaluation method for stable storage of milk hypnotize peptides |
| CN114720611A (en) * | 2022-04-02 | 2022-07-08 | 云南中烟工业有限责任公司 | Method for analyzing shelf life attenuation characteristics of aroma compounds of finished cigarette paper |
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