CN111436369A - Peanut bud stage cold tolerance identification method - Google Patents
Peanut bud stage cold tolerance identification method Download PDFInfo
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- CN111436369A CN111436369A CN202010284658.5A CN202010284658A CN111436369A CN 111436369 A CN111436369 A CN 111436369A CN 202010284658 A CN202010284658 A CN 202010284658A CN 111436369 A CN111436369 A CN 111436369A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The embodiment of the invention discloses a method for identifying cold resistance of peanut in a germination period, which comprises the following steps of disinfecting an appliance; selecting a plurality of peanut seeds, sterilizing the skins of the peanut seeds, and pretreating the sterilized peanut seeds to remove rotten seeds; cold resistance identification, namely uniformly dividing the pretreated peanut seeds into two parts, and respectively carrying out low-temperature culture and normal-temperature culture; data statistics and analysis, wherein the number of germinated seeds with the bud length exceeding the seed length in the experimental group and the control group is counted, and the relative germination rate is used as a cold tolerance index; the embodiment of the invention simulates actual conditions of field production, and carries out cold resistance screening and identification in the bud stage, and the screened low-temperature resistant germplasm is more reliable and useful; the method has the advantages that the epidermis of the peanut seeds is sterilized, and the sterilized peanut seeds are pretreated, so that misjudgment of poor cold resistance of the peanuts caused by the quality problems of the peanuts is avoided, and the accuracy of the result of cold resistance identification of the peanut seeds is improved.
Description
Technical Field
The embodiment of the invention relates to the technical field of peanut breeding, and particularly relates to a method for identifying cold resistance of peanuts in a germination period.
Background
Peanuts are a worldwide important oil and economic crop. The low temperature cold damage is one of the important factors limiting the development of the peanut industry. The peanuts are usually subjected to low-temperature cold damage after being sowed, and particularly, large-area rotten seeds are easy to occur when the peanuts are in a low-temperature condition for a long time after being imbibed and swelled, the emergence rate is obviously reduced, seedling shortage and ridge breaking are caused, and the yield is seriously reduced. The cultivation and planting of high-yield stable-yield variety with strong cold resistance is an ideal way for reducing the low-temperature cold damage, the lack of high-cold-resistance germplasm, the difficulty of cold resistance identification and the lack of molecular markers are one of the main reasons for limiting the breakthrough of cold resistance breeding, so that the problem of cold resistance identification of peanut germplasm in the bud stage is solved, and the method becomes a key factor for breakthrough of peanut cold resistance breeding and molecular markers.
The method for screening the cold-resistant resources of the peanuts closest to production is carried out by early sowing test in spring, so that the screened resources are in line with the actual production, but can only be carried out in the spring of northeast every year under the influence of seasons, and if the external temperature changes unstably, the test results are different. Therefore, many researchers adopt an indoor water culture method to screen cold-resistant resources, the method is carried out by adopting instruments such as an artificial climate box, an experimental group generally adopts a cold soaking temperature of 2 ℃ for 96 hours, and then the experimental group is placed in an incubator at 25 ℃ for dark culture, and seeding is started when the ground temperature is averagely stabilized at 12-15 ℃ in peanut production, the method identifies that the low-temperature resistant germplasm of the peanut does not accord with the actual production of the peanut, meanwhile, the seeds are not pre-screened before the traditional low-temperature stress culture, and the skin of some seeds is infected or the activity of the seeds is not high, so that the seeds are mildewed and then rotted, but the seeds are not mildewed and rotted due to low temperature, and the seeds are misjudged to be not cold-resistant and thus mildewed.
Disclosure of Invention
Therefore, the embodiment of the invention provides a method for identifying the cold tolerance of peanuts in the bud stage, which aims to solve the problems that in the prior art, the identification of the cold tolerance of peanuts does not conform to the practical production of the peanuts, and misjudgment of cold tolerance possibly caused by the fact that the peanut seeds are not subjected to screening does not influence the identification accuracy.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions:
a method for identifying the cold resistance of peanut in the bud stage comprises the following steps,
s100, sterilizing the apparatus, namely putting a culture dish wrapped by newspaper or a high-temperature resistant plastic bag, filter paper and a wide-necked bottle loaded with purified water into an autoclave for sterilization for later use;
s200, selecting seeds, selecting a plurality of mature and full peanut seeds with complete seed coats and consistent sizes, sterilizing the skins of the peanut seeds, and pretreating the sterilized peanut seeds to remove rotten seeds;
s300, cold resistance identification, namely uniformly dividing the pretreated peanut seeds into two parts, respectively putting the two parts into 2 culture dishes of which the bottom layers are covered with two layers of filter paper after sterilization, pouring sterilized purified water, wherein one part is marked as an experimental group, the other part is marked as a control group, the experimental group is firstly put into a 15 ℃ artificial climate box for dark culture for 72h, then is stressed at the low temperature of 2 ℃ for 72h, finally is put into a 25 ℃ artificial climate box for dark culture for 120h at constant temperature, and the control group is put into a 25 ℃ artificial climate box for dark culture at constant temperature;
s400, data statistics and analysis, wherein the number of the germinated seeds with the length of the buds exceeding the length of the seeds in the experimental group and the control group is counted, the low-temperature germination rate and the normal-temperature germination rate are respectively calculated, and the cold resistance of the peanut germplasm is identified by taking the ratio of the low-temperature germination rate to the normal-temperature germination rate, namely the relative germination rate as a cold resistance index.
The embodiment of the invention is further characterized in that in the step 200, the specific steps of sterilizing the skin of the peanut seeds are as follows: diluting 80% carbendazim wettable powder by 100 times, soaking peanut seeds in the diluent for 2 minutes, and cleaning the peanut seeds twice by using sterilized purified water.
The embodiment of the present invention is further characterized in that, in step 200, the specific steps of preprocessing the peanut seeds are as follows: placing the sterilized peanut seeds into a culture dish, wherein the bottom layer of the culture dish is covered with two layers of filter paper after sterilization, pouring sterilized purified water, placing the culture dish at normal temperature, and removing rotten peanut seeds after the peanut seeds are completely imbibed.
An embodiment of the present invention is further characterized in that, in step 400, the cold resistance index is: the peanut germplasm with the relative germination rate of more than or equal to 90 percent is taken as the high cold-resistant peanut germplasm, the peanut germplasm with the relative germination rate of 70-90 percent is taken as the cold-resistant germplasm, the peanut germplasm with the relative germination rate of 50-70 percent is taken as the intermediate germplasm, the peanut germplasm with the relative germination rate of 30-50 percent is taken as the sensitive germplasm, and the peanut germplasm with the relative germination rate of less than 30 percent is taken.
The culture dish provided by the embodiment of the invention is characterized by comprising a dish bottom and a dish wall, wherein the dish wall is detachably arranged around the outer edge of the dish bottom, a culture chamber is formed between the dish bottom and the dish wall in a surrounding manner, a plurality of independent culture chambers are arranged in the culture chamber in a partitioning manner, and a cutting blade for cutting and molding the filter paper is arranged at the joint of the dish bottom and the dish wall.
The embodiment of the invention is further characterized in that a circle of barrier strips are arranged on the outer edge of the dish bottom, a plurality of partition strips are longitudinally and transversely distributed on the upper surface of the dish bottom in the barrier strips, partition plates corresponding to the partition strips one by one are arranged in the dish wall, the cutting blades are distributed on the bottom of the partition plates and the lower edge of the dish wall, and blade slots matched with the cutting blades are formed in the barrier strips and the upper surfaces of the partition strips.
The embodiment of the invention is further characterized in that a dish cover is movably arranged above the dish wall, water leakage holes are formed in the positions, corresponding to each culture cavity, of the dish cover, and a liquid separating mechanism for uniformly injecting sterilizing water along the water leakage holes is arranged in the dish cover.
The embodiment of the invention is further characterized in that the liquid separating mechanism comprises a sterile sponge and a lower pressing plate, a concave cavity is arranged on the upper surface of the dish cover, the water leakage holes are distributed at the bottom of the concave cavity, the sterile sponge is arranged in the concave cavity, the lower pressing plate is arranged above the sterile sponge and used for extruding the sterile sponge, and a water injection hole used for injecting water to the sterile sponge is formed in the lower pressing plate.
The embodiment of the invention is further characterized in that the filter paper is arranged between the dish bottom and the dish wall, and the diameter of the filter paper is larger than that of the bottom surface of the dish bottom.
The embodiment of the invention has the following advantages:
(1) according to the embodiment of the invention, for an experimental group, experimental conditions of firstly placing the experimental group in a 15 ℃ artificial climate box for dark culture for 72h, then carrying out low-temperature stress for 72h at 2 ℃ and finally carrying out dark culture for 120h at 25 ℃ are adopted, the experimental conditions are in line with the actual production of peanuts, and sowing is started when the ground temperature is averagely stabilized at 12-15 ℃ in 5 days;
(2) according to the embodiment of the invention, the skin of the peanut seeds is sterilized before the cold resistance identification test, and the sterilized peanut seeds are pretreated to remove rotten seeds, so that misjudgment of poor cold resistance of the peanuts caused by the quality problem of the peanuts is avoided, and the accuracy of the result of the cold resistance identification of the peanut seeds is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
FIG. 1 is a flow chart of a method for identifying cold tolerance of peanut at a germination stage according to an embodiment of the present invention;
FIG. 2 is a schematic view of the overall structure of a culture dish according to an embodiment of the present invention;
FIG. 3 is a schematic view of the structure of the dish wall of the culture dish of FIG. 2;
FIG. 4 is a schematic structural view of the bottom of the dish in the culture dish shown in FIG. 2.
In the figure:
10-dish bottom; 20-dish wall; 30-a culture chamber; 40-a cutting blade; 50-a blade slot; 60-dish cover;
11-barrier strip; 12-parting beads;
21-a separator;
31-culture chamber;
61-water leakage holes; 62-sterile sponge; 63-a lower press plate; 64-a cavity; 65-water injection hole.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in figure 1, the invention provides a method for identifying the cold resistance of peanut in the bud stage, which comprises the following steps,
s100, disinfecting appliances;
placing a culture dish wrapped by newspaper or a high-temperature resistant plastic bag, filter paper and a wide-mouth bottle loaded with purified water into an autoclave for sterilization for later use;
s200, selecting seeds;
selecting a plurality of mature and full peanut seeds with complete seed coats and consistent sizes, sterilizing the skins of the peanut seeds, and pretreating the sterilized peanut seeds to remove rotten seeds;
in this step, the surface of the peanut seeds is sterilized because part of the surface of the peanut seeds is infected with bacteria, which causes the seeds to mildew and rot, but not the mildew and rot caused by low temperature, which is misjudged as not cold-resistant and causes the mildew and rot.
The specific steps of sterilizing the skin of the peanut seeds are as follows: the 80% carbendazim wettable powder is diluted by 100 times, then the peanut seeds are soaked in the diluent for 2 minutes, and the sterilized purified water is used for cleaning the peanut seeds twice, so that mildew and seed rot caused by other factors can be avoided.
The pretreatment of the peanut seeds comprises the following specific steps: placing the sterilized peanut seeds into a culture dish, wherein the bottom layer of the culture dish is covered with two layers of filter paper after sterilization, pouring sterilized purified water, placing the culture dish at normal temperature, and removing rotten peanut seeds after the peanut seeds are completely imbibed, thereby eliminating misjudgment of cold intolerance caused by the seeds with low activity.
S300, cold resistance identification, namely uniformly dividing the pretreated peanut seeds into two parts, respectively putting the two parts into 2 culture dishes of which the bottom layers are covered with two layers of filter paper after sterilization, pouring sterilized purified water, wherein one part is marked as an experimental group, the other part is marked as a control group, the experimental group is firstly put into a 15 ℃ artificial climate box for dark culture for 72h, then is stressed at the low temperature of 2 ℃ for 72h, finally is put into a 25 ℃ artificial climate box for dark culture for 120h at constant temperature, and the control group is put into a 25 ℃ artificial climate box for dark culture at constant temperature;
according to the embodiment of the invention, the experimental group is treated by placing the experimental group into a 15 ℃ artificial climate box for dark culture for 72h before low-temperature stress culture, the experimental group is in line with the practical production of peanuts, seeding is started when the ground temperature is averagely stabilized at 12-15 ℃ in 5 days, the practical conditions of field production are simulated, the cold resistance screening and identification in the bud stage are carried out, and the screened low-temperature resistant germplasm is more reliable and useful
S400, data statistics and analysis, wherein the number of the germinated seeds with the length of the buds exceeding the length of the seeds in the experimental group and the control group is counted, the low-temperature germination rate and the normal-temperature germination rate are respectively calculated, and the cold resistance of the peanut germplasm is identified by taking the ratio of the low-temperature germination rate to the normal-temperature germination rate, namely the relative germination rate as a cold resistance index.
The embodiment of the invention combines a control group, takes the relative germination percentage as the judgment standard of the cold resistance of germplasm, is simple, convenient and quick, and has practical significance, and the specific cold resistance indexes are as follows: the peanut germplasm with the relative germination rate of more than or equal to 90 percent is taken as the high cold-resistant peanut germplasm, the peanut germplasm with the relative germination rate of 70-90 percent is taken as the cold-resistant germplasm, the peanut germplasm with the relative germination rate of 50-70 percent is taken as the intermediate germplasm, the peanut germplasm with the relative germination rate of 30-50 percent is taken as the sensitive germplasm, and the peanut germplasm with the relative germination rate of less than 30 percent is taken.
In the embodiment of the present invention, the culture dish is mainly used as a container for culturing peanut seeds, the step 200 is used for eliminating the influence of the quality of the peanut seeds on the identification result, but once the mold in the seeds is not completely eliminated in the sterilization process, since a plurality of culture seeds are placed in one culture dish, the mold of the seeds is easily cross-infected and thus has a large influence on the whole experimental result, in order to reduce the influence, the embodiment of the present invention performs an isolation culture design on the culture dish, so that the single seeds are limited in a single space, and the liquids are not mutually exchanged, specifically:
as shown in fig. 2 to 4, the culture dish comprises a dish bottom 10 and a dish wall 20, the dish wall 20 is detachably arranged around the outer edge of the dish bottom 10, a culture chamber 30 is formed between the dish bottom 10 and the dish wall 20 in a surrounding manner, a plurality of independent culture chambers 31 are arranged in the culture chamber 30 in a separated manner, and due to the permeation effect of the filter paper, if the bottom of each culture chamber 31 is covered with one piece of filter paper, culture liquid is exchanged, so that the filter paper also needs to be cut into independent small pieces, the manual independent cutting is cumbersome, and preferably, a cutting blade 40 for cutting and forming the filter paper is arranged at the joint of the dish bottom 10 and the dish wall 20.
Further, a circle of barrier strips 11 are arranged on the outer edge of the dish bottom 10, a plurality of separation strips 12 are longitudinally and transversely distributed on the upper surface of the dish bottom 10 in the barrier strips 11, partition plates 21 in one-to-one correspondence with the separation strips 12 are arranged in the dish wall 20, the cutting blades 40 are distributed on the bottom of the partition plates 21 and the lower edge of the dish wall 20, and blade slots 50 matched with the cutting blades 40 are formed in the barrier strips 11 and the upper surfaces of the separation strips 12.
The assembly process of the culture dish after sterilization is as follows: firstly, filter paper is placed between the dish bottom 10 and the dish wall 20, the diameter of the filter paper is larger than the diameter of the bottom surface of the dish bottom 10, the cutting blade 40 at the bottom of the dish wall 20 is aligned to the blade slot 50 of the dish bottom 10, the filter paper can be cut into a plurality of pieces matched with the culture cavity 31 at one time through the cutting action of the cutting blade 40, meanwhile, the matching and embedding of the cutting blade 40 and the blade slot 50 also complete the assembly of the dish wall 20 and the dish bottom 10, and the culture chamber 30 is divided into a plurality of independent culture cavities 31 under the combined action of the partition strips 12 and the partition plates 21.
After the filter papers are correspondingly placed in the culture cavities 31 one by one, a certain amount of sterilization water needs to be added into each culture cavity 31 to soak the filter papers, but because the number of the culture cavities 31 is large, the sequential addition consumes a long time and can not ensure the consistency of the water level in each culture cavity 31, in the embodiment of the invention, a dish cover 60 is further movably covered above the dish wall 20, water leakage holes 61 are respectively arranged at the positions of the dish cover 60 corresponding to each culture cavity 31, and a liquid separation mechanism for uniformly injecting the sterilization water along the water leakage holes 61 is arranged in the dish cover 60.
Further, divide liquid mechanism to include aseptic sponge 62 and holding down plate 63, and ware lid 60 upper surface is provided with cavity 64, and the hole 61 that leaks distributes in the bottom of cavity 64, and aseptic sponge 62 is arranged in cavity 64, and holding down plate 63 sets up and is used for extrudeing aseptic sponge 62 in aseptic sponge 62's top, and offers the water injection hole 65 that is used for to aseptic sponge 62 water injection on the holding down plate 63.
When the liquid separating mechanism is used for watering the culture cavity 31, firstly, sterilized water is injected into the sterile sponge 62 along the water injection hole 65 to enable the sterile sponge 62 to be thoroughly wetted, the pressing plate 63 is pressed to drive the sterile sponge 62 to deform to release the water stored in the sterile sponge 62, the released water enters each corresponding culture cavity 31 along each water leakage hole 61, all the culture cavities 31 can be poured by one-time pressing, the watering amount is uniform, and the consistency of the experimental conditions of all the peanut seeds is ensured.
In addition, in order to ensure the accuracy of the results, in the embodiment of the present invention, multiple parallel samples can be designed for both the control group and the experimental group, for example, each treatment is set to be repeated three times, so as to ensure the repeatability and reproducibility of the experimental results.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (9)
1. A method for identifying the cold resistance of peanut in the bud stage is characterized by comprising the following steps,
s100, sterilizing the apparatus, namely putting a culture dish wrapped by newspaper or a high-temperature resistant plastic bag, filter paper and a wide-necked bottle loaded with purified water into an autoclave for sterilization for later use;
s200, selecting seeds, selecting a plurality of mature and full peanut seeds with complete seed coats and consistent sizes, sterilizing the skins of the peanut seeds, and pretreating the sterilized peanut seeds to remove rotten seeds;
s300, cold resistance identification, namely uniformly dividing the pretreated peanut seeds into two parts, respectively putting the two parts into 2 culture dishes of which the bottom layers are covered with two layers of filter paper after sterilization, pouring sterilized purified water, wherein one part is marked as an experimental group, the other part is marked as a control group, the experimental group is firstly put into a 15 ℃ artificial climate box for dark culture for 72h, then is stressed at the low temperature of 2 ℃ for 72h, finally is put into a 25 ℃ artificial climate box for dark culture for 120h at constant temperature, and the control group is put into a 25 ℃ artificial climate box for dark culture at constant temperature;
s400, data statistics and analysis, wherein the number of the germinated seeds with the length of the buds exceeding the length of the seeds in the experimental group and the control group is counted, the low-temperature germination rate and the normal-temperature germination rate are respectively calculated, and the cold resistance of the peanut germplasm is identified by taking the ratio of the low-temperature germination rate to the normal-temperature germination rate, namely the relative germination rate as a cold resistance index.
2. The method for identifying the cold tolerance of the peanut in the bud stage as claimed in claim 1, wherein in the step 200, the specific steps of sterilizing the skin of the peanut seed are as follows: diluting 80% carbendazim wettable powder by 100 times, soaking peanut seeds in the diluent for 2 minutes, and cleaning the peanut seeds twice by using sterilized purified water.
3. The method for identifying the cold tolerance of the peanut in the bud stage as claimed in claim 1, wherein the step 200 of pretreating peanut seeds comprises the following specific steps: placing the sterilized peanut seeds into a culture dish, wherein the bottom layer of the culture dish is covered with two layers of filter paper after sterilization, pouring sterilized purified water, placing the culture dish at normal temperature, and removing rotten peanut seeds after the peanut seeds are completely imbibed.
4. The method for identifying the cold tolerance of peanut in the bud stage as claimed in claim 1, wherein in step 400, the cold tolerance index is: the peanut germplasm with the relative germination rate of more than or equal to 90 percent is taken as the high cold-resistant peanut germplasm, the peanut germplasm with the relative germination rate of 70-90 percent is taken as the cold-resistant germplasm, the peanut germplasm with the relative germination rate of 50-70 percent is taken as the intermediate germplasm, the peanut germplasm with the relative germination rate of 30-50 percent is taken as the sensitive germplasm, and the peanut germplasm with the relative germination rate of less than 30 percent is taken.
5. The peanut bud-stage cold tolerance identification method according to claim 3, wherein the culture dish comprises a dish bottom (10) and a dish wall (20), the dish wall (20) detachably surrounds the outer edge of the dish bottom (10), a culture chamber (30) is surrounded between the dish bottom (10) and the dish wall (20), a plurality of independent culture cavities (31) are arranged in the culture chamber (30) in an isolated manner, and a cutting blade (40) for cutting and molding the filter paper is arranged at the joint of the dish bottom (10) and the dish wall (20).
6. The peanut germination period cold tolerance identification method according to claim 5, wherein a circle of barrier strips (11) are arranged on the outer edge of the dish bottom (10), a plurality of partition strips (12) are longitudinally and transversely distributed on the upper surface of the dish bottom (10) in the barrier strips (11), partition plates (21) corresponding to the partition strips (12) one to one are arranged in the dish wall (20), the cutting blades (40) are distributed on the bottoms of the partition plates (21) and the lower edge of the dish wall (20), and blade slots (50) matched with the cutting blades (40) are formed in the upper surfaces of the barrier strips (11) and the partition strips (12).
7. The peanut bud stage cold tolerance identification method according to claim 5, wherein a dish cover (60) is movably covered above the dish wall (20), water leakage holes (61) are formed in the positions, corresponding to each culture cavity (31), of the dish cover (60), and a liquid separation mechanism for uniformly injecting sterilizing water along the water leakage holes (61) is arranged in the dish cover (60).
8. The peanut bud stage cold tolerance identification method according to claim 7, wherein the liquid distribution mechanism comprises a sterile sponge (62) and a lower pressing plate (63), a concave cavity (64) is formed in the upper surface of the dish cover (60), the water leakage holes (61) are distributed in the bottom of the concave cavity (64), the sterile sponge (62) is arranged in the concave cavity (64), the lower pressing plate (63) is arranged above the sterile sponge (62) and used for extruding the sterile sponge (62), and water injection holes (65) used for injecting water into the sterile sponge (62) are formed in the lower pressing plate (63).
9. The method for identifying the cold resistance of peanut in the bud stage according to claim 6, wherein the filter paper is placed between the dish bottom (10) and the dish wall (20), and the diameter of the filter paper is larger than the diameter of the bottom surface of the dish bottom (10).
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