CN111426832A

CN111426832A - Sample detection device and sample detection method

Info

Publication number: CN111426832A
Application number: CN202010272982.5A
Authority: CN
Inventors: 宋禹
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-04-09
Filing date: 2020-04-09
Publication date: 2020-07-17

Abstract

The invention discloses a sample detection device and a sample detection method. The sample detection device includes: the device comprises a first container, a second container, a sample bin, a sample processing bin and a reaction bin; the sample bin is used for containing samples; the sample treatment bin is used for mixing the sample treatment fluid with a sample to obtain a first mixed solution; the reaction bin is used for mixing the first mixed solution with the reaction solution to obtain a second mixed solution; the first container is connected with the sample processing bin through a second pipeline; the sample bin is connected with the sample processing bin through a first pipeline: the sample processing bin is connected with the reaction bin through a third pipeline; the second container is connected with the reaction bin through a fourth pipeline; the first container is provided with a processing liquid, the second container is provided with a reaction liquid, and the first container can be pierced by the first ejection part, so that the processing liquid flows into the sample processing bin through the second pipeline; the second container can be pierced by the second ejection portion, so that the reaction liquid flows into the reaction bin through the fourth pipeline. The sample collecting device is simple and exquisite in structure, and solves the technical problems that sample collection, sample processing and detection cannot be performed uniformly, and detection efficiency is low in the prior art.

Description

Sample detection device and sample detection method

Technical Field

The invention relates to the field of biological detection, in particular to a sample detection device and a sample detection method.

Background

With the continuous development of intelligent convenient biological detection devices, detection means are more and more abundant when people encounter diseases. For example, when a hospital needs to collect human blood and detect the presence of pneumovirus, a portable sample collector, i.e., a disposable blood collection device and a blood storage device, is often used to collect a sample of patient blood, and the blood sample is used in combination with a sample analyzer to detect the presence of virus antibody antigen in the sample of patient blood.

At present, a detection device for detecting protein, ester, polysaccharide and gene usually mixes a sample with a sample treatment solution, and the mixed solution is separately subjected to subsequent treatment, so that the whole detection process is complicated and low in efficiency, and the sample is always polluted to a certain extent in the process of being continuously conveyed, so that the detection result is inaccurate.

In view of the above problems, no effective solution has been proposed.

Disclosure of Invention

The embodiment of the invention provides a sample detection device and a sample detection method, which at least solve the technical problems that the prior art cannot uniformly carry out sample storage, sample processing and detection and has low detection efficiency.

According to an aspect of an embodiment of the present invention, there is provided a sample testing device including a first container, a second container, a sample chamber, a sample processing chamber, and a reaction chamber; the sample bin is used for containing samples; the sample treatment bin is used for mixing the sample treatment liquid with a sample to obtain a first mixed liquid; the reaction bin is used for mixing the first mixed solution with a reaction solution to obtain a second mixed solution; the first container is connected with the sample processing bin through a second pipeline; the sample bin is connected with the sample processing bin through a first pipeline: the sample processing bin is connected with the reaction bin through a third pipeline; the second container is connected with the reaction bin through a fourth pipeline; the first container is provided with a processing liquid, the second container is provided with a reaction liquid, and the first container can be punctured by the first ejection part, so that the processing liquid flows into the sample processing bin through the second pipeline; the second container can be pierced by the second ejection part, so that the reaction liquid flows into the reaction bin through the fourth pipeline.

According to another aspect of the embodiments of the present invention, there is also provided a sample detection method, including: keeping the air hole of the reaction bin in a closed state, opening the air hole of the sample processing bin, and after the first pressing part is pressed down, piercing the first container by the first ejection part to enable the sample processing liquid to enter the sample processing bin through the second pipeline;

after a sample is added into the sample bin, the second air hole is in a closed state, and the third pipeline is in a non-communicated state, so that the sample stays in the sample bin;

after the pressure providing part is pressed, the sample chamber is inflated, a sample enters the sample processing chamber through the first pipeline and fills the sample processing chamber from bottom to top, and air holes of the first container and the second container are kept closed;

closing the first air hole of the sample processing bin and opening the second air hole of the reaction bin to ensure that the liquid in the sample processing bin enters the reaction bin due to air pressure;

after the second container is punctured, the reaction liquid in the second container enters the reaction bin along the fourth pipeline;

after the pressure providing part is pressed for multiple times, gas enters the reaction bin through the first pipeline and the third pipeline in view of the sealing of the first air hole in the sample processing bin, so that the sample processing liquid and the reaction liquid are fully mixed and react;

and detecting according to the reaction result.

In the embodiment of the invention, a mode of integrating sample processing, sample mixing and sample detection is adopted, and the control of the pressure device and the conveying pipeline is utilized to achieve the purposes of fully mixing and processing samples and efficiently and accurately detecting the samples, so that the technical problems that in the prior art, sample storage, sample processing and detection cannot be uniformly carried out, and the detection efficiency is low are solved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:

FIG. 1 is a schematic diagram of the internal structure of a sample testing device according to an embodiment of the present invention;

FIG. 2 is a schematic view of a sample testing device including a housing.

FIG. 3 is a flow chart of a method of sample detection according to an embodiment of the invention.

Fig. 4 is a detailed flow chart of the operational steps according to an embodiment of the present invention.

Detailed Description

In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

In accordance with an embodiment of the present invention, there is provided a method embodiment of sample detection, it should be noted that the steps illustrated in the flowchart of the figure may be performed in a computer system such as a set of computer executable instructions, and that while a logical order is illustrated in the flowchart, in some cases the steps illustrated or described may be performed in an order different than here.

The in-process about gathering sample analysis and handling sample of prior art, the main device that the sample was placed as the sample to sample collection and storage pipe has been utilized usually, then through adding sample treatment fluid or reaction liquid to the sample collection pipe, and mix through machine or manual work, reach the purpose that detects the sample, but prior art detects the mode of sample and has the detection space not sealed, the sample contacts the outside air and leads to the testing result inaccurate, it is loaded down with trivial details complicated to have the detection step simultaneously, the inconvenient scheduling problem of operation, lead to true sample detection process inefficiency.

Example one

Fig. 1 is a schematic view of an internal structure of a sample test device according to an embodiment of the present invention, as shown in fig. 1, the device including: a first container 41, a second container 42, a sample chamber 10, a sample processing chamber 20, and a reaction chamber 30;

wherein, the sample bin 10 is used for containing samples; the sample processing bin 20 is used for mixing the sample processing liquid with a sample to obtain a first mixed liquid; the reaction bin 30 is used for mixing the first mixed solution with a reaction solution to obtain a second mixed solution; the first container 41 is connected to the sample processing chamber 20 through a second pipeline 52; the sample chamber 10 is connected to the sample processing chamber 20 through a first pipeline 51: the sample processing bin 20 is connected with the reaction bin 30 through a third pipeline 53; the second container 42 is connected with the reaction bin 30 through a fourth pipeline 54; the first container 41 has a processing liquid therein, the second container 42 has a reaction liquid therein, and the first container 41 can be pierced by a first ejection portion 71 disposed on the housing or the outside, so that the processing liquid flows into the sample processing chamber 20 through the second pipeline 52; the second container 42 can be pierced by a second ejection portion 72 disposed at the housing or the outside, so that the reaction liquid flows into the reaction chamber 30 through the fourth pipeline 54. The first and second liftout portions 72 will be described in detail in fig. 2.

FIG. 2 is a schematic view of a sample testing device including a housing. As shown in FIG. 2, optionally, the sample testing device further includes a housing 90, which may be made of plastic, and for clarity, the components of the housing shown in FIG. 2 are marked with thicker lines. The housing 90 comprises a sample inlet 91 corresponding to the opening of the sample chamber 10 for injecting a sample liquid. The sample liquid may fill the sample processing chamber 20 from below up the first conduit 51 shown in fig. 2. In addition, the housing 90 further has a first pressing portion 92 and a second pressing portion 93, and the first pressing portion 92 is linked with the first ejecting portion 71 to pierce the first container 41. The second pressing portion 93 is linked with the second ejecting portion 72 for piercing the second container 42. The first pressing portion 92 and the first ejecting portion 71 may be integrally formed or assembled, and the second pressing portion 93 and the second ejecting portion 72 may be integrally formed or assembled.

The first pressing portion 92 and the second pressing portion 93 are used for generating elastic deformation to drive at least one of the first ejecting portion 71 and the second ejecting portion 72 to pierce at least one of the first container 41 and the second container 42. The first pressing part is arranged corresponding to the first container 41 and is used for being pressed by a user or a machine to pierce the first container 41 by using the first ejection part 71; the second pressing portion is a structure provided corresponding to the second container 42 and used for a user or a machine to press and pierce the second container 42 with the second ejection portion 72.

It should be noted that fig. 1 and 2 are schematic views showing a use state of the sample testing device, that is, a state in which the sample testing device is upright, and the "up and down" described herein are illustrated by taking the up and down in the use state as an example, that is, the upright state of the sample testing device as an example, and are not particularly limited.

Specifically, the first pressing portion 91 and the second pressing portion 92 may be made of an elastic material, such as a plastic elastic sheet. In the case of non-integral molding, the first ejection portion 71 and the second ejection portion 72 used for piercing the first container 41 and the second container 42 may be rigid piercing pins, and when the tablet made of elastic material deforms, the ejection portion on the tablet pierces the container immediately, so as to ensure that the liquid in the container is also conveyed out for the subsequent detection processing process while the tablet deforms under the influence of external force.

Alternatively, at least one of the first container 41 and the second container 42 is made of aluminum foil.

Specifically, the first container 41 and the second container 42 can be loaded with the sample processing solution and the reaction solution, respectively, and the embodiment of the present invention employs an aluminum foil as the material of the first container and the second container, which is easily broken due to its soft texture and facilitates the piercing of the ejection part because the container achieves the best piercing response effect when the aluminum foil is used as the container material. It is worth noting that in order to prevent the two layers from being pierced when the aluminum foil is used as a material, a hard bulge can be arranged on one layer of the aluminum foil bag, and the two layers of the aluminum foil bag are prevented from being pierced by the ejection part at the same time. In order to prevent pressing the inhomogeneous problem of aluminium foil bag, can set up the shape into the adaptation aluminium foil bag with pressing the splenium, avoid pressing the atress inequality.

Optionally, the reaction liquid includes a reaction buffer or a reaction reagent.

Specifically, the reaction solution may be a reaction buffer solution or a reaction reagent, wherein the reaction buffer solution needs to be combined with the lyophilized pellet to dissolve the lyophilized pellet, and is mixed with the first mixed solution to react. For example, after the tester punctures the second container 42 by hand or by machine, the reaction buffer solution in the second container enters the reaction chamber 30 along the fourth pipeline 54, and is mixed with the lyophilized pellet, so that the lyophilized pellet is dissolved. For another example, in other cases, the second container 42 stores the reaction reagent, and the reaction reagent in the second container 42 is directly extruded into the reaction chamber 30 without the lyophilized powder pellets in the reaction chamber 30. Optionally, the sample chamber 10 is tapered, and a cover for sealing the sample chamber 10 is disposed at the top of the sample chamber 10, so that the sample liquid in the sample chamber 10 in the sealed state cannot flow along the first pipeline 51.

Optionally, the sample processing chamber 20 has a first air vent for preventing the liquid in the sample processing chamber 20 from flowing along the third pipeline 53 in the blocking state. The reaction chamber 30 has a second air hole, and the liquid in the reaction chamber cannot flow along the fourth pipeline when the second air hole is in a closed state.

Optionally, in the use state, the position where the second pipeline 52 is communicated with the sample processing chamber 20 is higher than the position where the first pipeline 51 is communicated with the sample processing chamber 20. That is, the second pipe 52 communicates with the sample treatment chamber 20 at a position closer to the air hole of the reaction chamber 20 than the first pipe 51 communicates with the sample treatment chamber 20.

Optionally, the sample detection device further comprises an air hole blocking device for blocking at least one of the first air hole and the second air hole. The number of the plugging means is two, for example, for plugging the first air hole 61 and the second air hole 62, respectively.

Specifically, the blocking device may be a plastic cap that is in interference fit with the air hole, and the air hole may be opened or closed by pulling out and inserting the plastic cap.

Optionally, as shown in fig. 1, the sample detection apparatus further includes: and a pressure providing part 80 connected to the sample chamber 10 through a pressure providing line for providing a gas pressure to mix the sample in the sample chamber 10 with the sample processing liquid in the sample processing chamber 20. Corresponding to the pressure providing part 80, as shown in fig. 2, the housing has a pressure providing pressing piece 94 thereon for a user or a machine to apply pressure. The pressure supply part 80 and the pressure supply pressing member 94 function to change the shape of the pressure supply part by pressing the pressing button, and gas enters the sample chamber 10 from a gas pipeline connected to the sample chamber 10, and liquid in the sample chamber 10 is pressed into the sample processing chamber 20 through the first pipeline 51 to be sufficiently mixed with the sample processing liquid therein.

Alternatively, the pressure providing part 80 and the pressure providing pressing piece 94 are resilient materials, so that the pressure providing part 80 and the pressure providing pressing piece 94 can be resiliently returned after being pressed.

Alternatively, the first to fourth lines 51 to 54 are formed on the housing, and the sample processing chamber 20, the reaction chamber 30, and the first and second containers 41 and 42 are disposed in the housing. The housing may comprise, for example, a first housing and a second housing. The first casing 90 is denoted as a first casing, a second casing and a first casing for accommodating the sample processing chamber 20, the reaction chamber 30, the first container 41 and the second container 42, the first pipeline 51 to the fourth pipeline 54, and the pressure supply pipeline, for example, are flow channels integrally formed on the second casing, and the pipelines are formed by the aligned engagement of the first casing and the second casing. The housing 90, such as the first housing or the second housing, also has seals for sealing the plurality of conduits. The exterior of the pipe is sealed with a sealing material such as rubber.

According to another aspect of the embodiments of the present invention, there is also provided a sample testing terminal for adapting and installing the above sample testing device, the sample testing terminal comprising a receiving portion for receiving the sample testing device, and an air hole blocking device for blocking the first air hole and the second air hole. The accommodating portion is, for example, a groove capable of accommodating the sample detection device.

According to another aspect of the embodiments of the present invention, the sample testing terminal further comprises a testing result display device for generating and displaying a testing result according to the second mixed liquid, and an electric component for pressing the pressure providing part.

Example two

According to another aspect of the embodiments of the present invention, there is also provided a sample detection method, including: injecting the sample processing liquid into the sample processing chamber 20; mixing the sample treatment solution with a sample to obtain a first mixed solution; injecting the first mixed solution into a sample reaction bin 30; mixing the first mixed solution with a reaction solution to obtain a second mixed solution; and generating a detection result according to the second mixed solution.

Fig. 3 is a flow chart of a sample testing method according to an embodiment of the present invention, as shown in fig. 3, including:

in step S301, the sample processing liquid is injected into the sample processing chamber 20.

Specifically, the embodiment of the present invention aims to realize processes of storing a sample and analyzing the sample after the sample is collected from a case or a sample source, so that in order to perform a preliminary sample liquid treatment on the collected sample liquid, a sample treatment liquid needs to be mixed with the sample to achieve a purpose of subsequently treating the sample.

As shown in fig. 1, the air hole of the reaction chamber 30 is kept closed, the air hole of the sample processing chamber 20 is opened, the first container is pressed, the first ejector pierces the first container, and the sample processing liquid in the first container, which is required to be mixed with the collected sample and analyzed, enters the sample processing chamber 20 through the second pipe 52, and the air hole is opened in order to inject the sample processing liquid into the processing chamber by using the atmospheric pressure in the pipe.

Optionally, the injecting the sample processing liquid into the sample processing bin 20 includes: opening the first air vent 61 of the sample processing cartridge 20; the sample processing liquid is injected from the sample processing liquid container to the sample processing chamber 20.

It should be noted that the container in which the sample processing solution is located may be an aluminum foil bag, and the aluminum foil facilitates penetration of a push-out portion (e.g., a broken needle) to form a vacuum to release the sample processing solution loaded in the aluminum foil bag.

Step S302, mixing the sample treatment fluid with a sample to obtain a first mixed solution.

As shown in fig. 3, the sample chamber 10 is inflated by pressing the pressure supply portion 80, so that the sample enters the sample processing chamber 20 through the first pipe 51, and fills the sample processing chamber 20 from bottom to top, but does not overflow through the opening of the second pipe 52. At this time, the air holes of the first container and the second container are closed, and the sample processing liquid does not flow down along the third pipe 53.

Optionally, before mixing the sample treatment solution with the sample to obtain a first mixed solution, the method further includes: the sample is added to the sample bin 10.

Specifically, the sample chamber 10 may be a conical structure, which is convenient for the inspector to put the collected sample liquid into the sample chamber 10.

Step S303, the first mixed liquid is injected into the sample reaction chamber 30.

Specifically, as shown in fig. 1, the first air hole of the sample processing chamber 20 is closed, and the second air hole of the reaction chamber 30 is opened, so that the liquid in the sample processing chamber 20 enters the reaction chamber 30 due to the air pressure, wherein the liquid in the sample processing chamber 20 is the first mixed liquid, that is, the first mixed liquid is injected into the reaction chamber 30 to wait for further reaction and detection through the control manner of the air pressure of the air holes.

Step S304, mixing the first mixed solution with a reaction liquid to obtain a second mixed solution.

Specifically, after the first mixed liquid enters the reaction chamber 30, the reaction liquid also needs to be injected into the reaction chamber 30 to react with the first mixed liquid, so as to obtain the detection result. The container in which the reaction solution is located may be a second container, such as an aluminum foil bag, and the aluminum foil is punctured by the same needling method as the sample processing solution, so that the reaction solution is pneumatically transferred into the reaction chamber 30.

Specifically, as shown in fig. 1, by pressing the pressure supply part 80 multiple times while the first air hole in the sample processing chamber 20 is closed, the air can enter the reaction chamber 30 only through the first and third pipes 51 and 53, so that the sample processing solution and the reaction reagent are sufficiently mixed. In this process, the first container 41 and the second container 42 are kept stationary after the liquid is punctured and pressed out, and the air pressure closes the liquid discharge pipes of the first container 41 and the second container 42, so that the liquid is prevented from flowing back.

Optionally, when the reaction solution is the reaction buffer solution, the method further includes: the reaction buffer was mixed with the lyophilized pellet and dissolved.

Specifically, the reaction solution may be a reaction buffer or a reaction reagent, wherein the reaction buffer needs to be combined with the lyophilized pellet and mixed with the first mixture. For example, after the inspector punctures the second container 42, the reaction buffer solution in the second container enters the reaction chamber 30 along the fourth pipeline 54 and is mixed with the lyophilized pellet to dissolve the lyophilized pellet. For another example, in other cases, the second container 42 stores the reaction reagent, and the reaction reagent in the second container 42 is directly extruded into the reaction chamber 30 without the lyophilized powder pellets in the reaction chamber 30.

Step S305, generating a detection result according to the second mixed solution.

Specifically, a detection module may be disposed in the reaction bin 30, and colorimetric detection or turbidity detection is performed on a detection result of the reaction bin 30, so as to detect a reaction result in the reaction bin 30, and the detection result may be fed back to the intelligent terminal carrier, where the intelligent terminal carrier may be a PC terminal or a mobile terminal with a detection device slot.

FIG. 4 is a flow chart showing the operation steps of the present invention. As shown in fig. 4, the operation steps of the present invention include the following steps:

step S401, keeping the air hole of the reaction chamber 30 in a closed state, opening the second air hole 61 of the sample processing chamber 20, after pressing down the first pressing part of the shell, piercing the first container 41 by the first ejector 71, and allowing the sample processing liquid in the first container 41 to enter the sample processing chamber 20 through the second pipeline 52;

step S402, add the sample into the sample chamber, as shown in fig. 2, at this time, the second air hole 62 is in a closed state, and the third pipeline 53 is not connected, so that the sample stays in the sample chamber 10.

In step S403, the sample chamber 10 is inflated by pressing the pressure providing part 80 of fig. 3, so that the sample enters the sample processing chamber 20 through the first pipeline 51, and fills the sample processing chamber 20 from bottom to top, but does not overflow through the opening of the second pipeline. The air holes of the first container 41 and the second container 42 are now kept closed, and the sample processing liquid does not flow down the third pipe. The pressure providing part 80 is pressed several times, and the processing liquid and the sample in the sample processing chamber 20 are sufficiently mixed.

Step S404, closing the first air hole 61 of the sample processing chamber 20, opening the second air hole 62 of the reaction chamber, and allowing the liquid in the sample processing chamber 20 to enter the reaction chamber 30 due to the air pressure.

Step S405, puncturing the second container 42, so that the reaction liquid in the second container enters the reaction chamber 30 along the fourth pipeline, and is mixed with the lyophilized pellet in the reaction chamber, so that the lyophilized pellet is dissolved. In other cases, the second container 42 stores the reagents, and the reagents in the second container 42 are directly extruded into the reaction chamber 30 without the need of freeze-dried powder pellets in the reaction chamber.

In step S406, after the pressure providing part is pressed multiple times, the gas can enter the reaction chamber only through the first and third pipelines 51 and 53 in view of the sealing of the first air hole 61 in the sample processing chamber, so that the sample processing solution and the reaction reagent are mixed sufficiently. In this process, the first container 41 and the second container 42 are kept stationary after the liquid is punctured and pressed out, and the air pressure closes the liquid discharge pipes of the first container 41 and the second container 42, so that the liquid is prevented from flowing back.

And step S407, detecting according to the reaction result.

In the embodiments provided in the present application, it should be understood that the disclosed technology can be implemented in other ways. The above-described embodiments of the apparatus are merely illustrative, and for example, the division of the units may be a logical division, and in actual implementation, there may be another division, for example, multiple units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some interfaces, units or modules, and may be in an electrical or other form.

The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units, may be located in one place, or may be distributed on a plurality of units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.

In addition, functional units in the embodiments of the present invention may be integrated into one processing unit, or each unit may exist alone physically, or two or more units are integrated into one unit. The integrated unit can be realized in a form of hardware, and can also be realized in a form of a software functional unit.

The integrated unit, if implemented in the form of a software functional unit and sold or used as a stand-alone product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present invention may be embodied in the form of a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a removable hard disk, a magnetic or optical disk, and other various media capable of storing program codes.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The sample detection device is characterized by comprising a first container, a second container, a sample bin, a sample processing bin and a reaction bin;

the sample bin is used for containing samples; the sample treatment bin is used for mixing the sample treatment liquid with a sample to obtain a first mixed liquid; the reaction bin is used for mixing the first mixed solution with a reaction solution to obtain a second mixed solution; the first container is connected with the sample processing bin through a second pipeline; the sample bin is connected with the sample processing bin through a first pipeline: the sample processing bin is connected with the reaction bin through a third pipeline; the second container is connected with the reaction bin through a fourth pipeline;

the first container is provided with a sample processing liquid, the second container is provided with a reaction liquid, and the first container can be punctured by the first ejection part, so that the sample processing liquid flows into the sample processing bin through the second pipeline and reacts with the sample entering the sample processing bin; the second container can be pierced by the second ejection part so that the reaction liquid flows into the reaction chamber through the fourth pipeline.

2. The device of claim 1, wherein the sample testing device further comprises a housing, the housing has a first pressing portion and a second pressing portion, the first pressing portion can be elastically deformed to drive the first ejection portion to pierce the first container, so that the liquid in the first container flows into the sample processing chamber; the second pressing part can elastically deform to drive the second ejection part to pierce the second container, so that the liquid in the second container flows into the reaction bin.

3. The device of claim 1, wherein at least one of the first container and the second container is made of aluminum foil.

4. The apparatus of claim 1, wherein the top of the sample chamber has a cover that seals the sample chamber such that the sample chamber cannot flow along the first conduit when the sample chamber is in a sealed state.

5. The device of claim 1, wherein in a use state, the second conduit is in communication with the sample processing compartment at a higher level than the first conduit is in communication with the sample processing compartment.

6. The apparatus of claim 1, wherein the sample processing chamber has a first vent, wherein in the closed state, liquid in the sample processing chamber cannot flow along the third conduit; the reaction bin is provided with a second air hole, and liquid in the reaction bin cannot flow along the fourth pipeline when the second air hole is in a blocking state.

7. The apparatus of claim 6, wherein the sample testing device further comprises an air hole blocking device for blocking at least one of the first air hole and the second air hole.

8. The apparatus of claim 1, wherein the sample testing apparatus further comprises: and the pressure providing part is connected with the sample bin and is used for providing air pressure so that the sample in the sample bin is mixed with the sample processing liquid in the sample processing bin.

9. The device of claim 8, wherein the pressure provider is a resilient material such that the pressure provider can rebound upon depression.

10. The apparatus of claim 2, wherein the first through fourth conduits are formed on the housing, and the sample processing cartridge, the reaction cartridge, and the first and second containers are disposed within the housing.

11. The apparatus of claim 10, wherein the housing further has a seal for sealing the plurality of conduits.

12. A sample testing terminal adapted to fit a sample testing device according to any of claims 1 to 11, wherein said terminal comprises a receptacle for receiving said sample testing device and a vent closure means for closing off said first vent and said second vent.

13. The terminal of claim 12, wherein the sample testing terminal further comprises a testing result display device for generating and displaying a testing result according to the second mixture.

14. The terminal of claim 13, further comprising an electro-motive member for depressing the pressure providing portion.

15. A method for detecting a sample, comprising:

keeping the air hole of the reaction bin in a closed state, opening the air hole of the sample processing bin, and after the first pressing part is pressed down, the first ejector piece punctures the first container so that the sample processing liquid enters the sample processing bin through the second pipeline;

after the pressure providing part is pressed for many times, gas enters the reaction bin through the first pipeline and the third pipeline on the premise that the first air hole in the sample processing bin is closed, so that sample processing liquid and reaction liquid are fully mixed and react;

and detecting according to the reaction result.