CN111423976B - Aseptic inoculation device of microorganism - Google Patents
Aseptic inoculation device of microorganism Download PDFInfo
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- CN111423976B CN111423976B CN202010276751.1A CN202010276751A CN111423976B CN 111423976 B CN111423976 B CN 111423976B CN 202010276751 A CN202010276751 A CN 202010276751A CN 111423976 B CN111423976 B CN 111423976B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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Abstract
The invention discloses a microorganism aseptic inoculation device, which comprises: the device comprises an outer cover, a tube placing mechanism and a tube positioning mechanism, wherein the tube placing mechanism is arranged in the outer cover and is used for placing a microorganism test tube and driving the microorganism test tube to rotate so as to incline the microorganism test tube; the plug pulling mechanism is arranged in the outer cover and used for pulling out a test tube plug of the microorganism test tube; the sterilization mechanism is arranged in the outer cover and is used for sterilizing the pipe orifice of the microorganism test tube; the inoculation mechanism is arranged at the upper end of the outer cover and is used for transferring strains in the microbial test tube into a culture dish; and the dish placing unit is arranged on the substrate in the outer cover and used for placing a culture dish. The invention improves the efficiency of microbial inoculation, can effectively prevent other microbes from influencing target strains, has simple operation, can effectively reduce the possibility of infection of the target strains to operators, and has stronger practicability.
Description
Technical Field
The invention relates to the technical field related to microbial inoculation, in particular to a microbial sterile inoculation device.
Background
In the microbial research, in the experimental stage, in order to avoid the influence of other types of microorganisms on the research of target strains, therefore, in the inoculation process, the inoculation process needs to be ensured to be aseptic operation, currently, various ways are adopted to reduce the influence of other types of microorganisms on the target strains, for example, in the microbial inoculation process, a high-temperature sterilization way is adopted to place the influence of normal-temperature strains on the target strains, namely, in the strain inoculation process, an alcohol lamp is placed in the operation process, various inoculation tools are burned by the alcohol lamp before inoculation, so that the sterilization effect is achieved, and in the inoculation process, an inoculated container needs to be placed around the alcohol lamp, so that other strains in the air are burned by the alcohol lamp.
Although the prior art can achieve aseptic operation, the requirement on the proficiency of operators is high, and the inoculation mode is performed in an over-sterilized environment, but the probability that target strains are polluted in the inoculation process is increased due to the fact that the operators carry more other strains, and meanwhile, the inoculation mode is low in efficiency, and even the target strains are diffused due to improper operation of the operators, so that the influence on the bodies of the operators is possibly generated.
Disclosure of Invention
The invention provides a microbial sterile inoculation device, which is used for solving the defects of the prior art, improving the efficiency of microbial inoculation, effectively preventing other microbes from influencing target strains, being simple to operate, effectively reducing the possibility of infection of the target strains to operators and having strong practicability.
In order to achieve the purpose of the invention, the following technology is adopted:
a microbial sterile inoculation device comprising:
the outer cover is provided with a plurality of grooves,
the tube placing mechanism is arranged in the outer cover, is used for placing the microorganism test tube and driving the microorganism test tube to rotate so as to incline the microorganism test tube, and comprises a base plate arranged on a bottom plate of the outer cover;
the plug pulling mechanism is arranged in the outer cover and used for pulling out a test tube plug of the microorganism test tube;
the sterilization mechanism is arranged in the outer cover and is used for sterilizing the pipe orifice of the microorganism test tube;
the inoculation mechanism is arranged at the upper end of the outer cover and is used for transferring strains in the microbial test tube into a culture dish;
the dish placing unit is arranged on the substrate in the outer cover and used for placing a culture dish;
the sterilization mechanism and the dish placing unit are respectively positioned at two ends of the outer cover, the pipe placing mechanism is positioned between the sterilization mechanism and the dish placing unit, the plug pulling mechanism is positioned above the pipe placing mechanism, and the inoculation mechanism moves along the length direction of the outer cover and is used for inoculation of microorganisms.
Further, put tub mechanism including being the support piece of equidistant array ground installation in base plate one end, the support piece upper end all takes shape there is the concave groove, the concave inslot all is equipped with the connecting piece, the bull stick is all installed to connecting piece one end, the bull stick assembles in the support piece upper end, the one end of bull stick is connected with the rotation cylinder, the rotation cylinder is installed in the dustcoat, the connecting piece other end all installs double-layered pipe assembly, double-layered pipe assembly is used for the fixed microorganism test tube of centre gripping, the lower extension pole is installed to the connecting piece all downwardly extending ground, the hood down is installed to lower extension pole lower extreme, microorganism test tube lower extreme is arranged in the hood down.
Furthermore, the connecting piece comprises a rotating block assembled at the concave groove, and the other end of the rotating block is provided with a fixed connecting plate.
Furthermore, the pipe clamping assembly comprises a first pipe clamping arc plate arranged at the other end of the fixed connecting plate, first lugs are formed at two ends of the first pipe clamping arc plate, telescopic rods penetrate through the first lugs, end plugs are formed at one ends of the telescopic rods, rotating shafts are assembled at the other ends of the telescopic rods, rotating pieces are assembled on the rotating shafts, clamping blocks are formed on the outer arc walls of the rotating pieces, pressing plates are further formed on the outer arc walls of the rotating pieces, and the pressing plates are perpendicular to the clamping blocks;
the pipe clamping assembly further comprises a second pipe clamping arc plate, second lugs are formed at two ends of the second pipe clamping arc plate, and the second lugs penetrate through the telescopic rod;
when the clamping tube assembly clamps and fixes the microorganism test tube, the rotating piece rotates under the action of the pressing plate, so that the other end of the clamping block acts on the second lug;
rubber pad bulges are arranged on the inner walls of the first pipe clamping arc plate and the second pipe clamping arc plate.
Further, the plug pulling mechanism is including installing the lift cylinder on the top of the dustcoat, the lifter plate is installed to the expansion end of lift cylinder, the lifter plate both sides are installed the installation and are prolonged the board a plurality ofly, the dead lever is installed to the installation and prolong the equal downwardly extending ground of the board other end, the centre gripping assembly plate is all installed to the dead lever that is located the homonymy, the equal shaping of centre gripping assembly plate has the pilot hole, the pilot hole all is equipped with the grip block, the grip block is L shape structure, the centre gripping cylinder is all installed to the grip block, the cylinder mounting panel is installed to the centre gripping cylinder other end, the cylinder mounting panel is all installed in the centre gripping assembly plate.
Furthermore, the sterilization mechanism comprises a plurality of lifting assemblies arranged at the other end of the substrate, the lifting assemblies are all provided with telescopic assemblies, and the sterilization assemblies are all arranged at the other ends of the telescopic assemblies;
the lifting assembly comprises a plurality of extension mounting plates arranged at one end of the base plate, and lifting screw rods are arranged at the other ends of the extension mounting plates in an upward extending manner;
the inside baffle that is equipped with of dustcoat, the baffle is formed and is perforated a plurality of, and flexible subassembly passes in the perforation.
Further, flexible subassembly is including assembling in elevating screw's lift assembly plate, and the installation fixed plate is installed to lift assembly plate one end, and telescopic cylinder is installed to the installation fixed plate, and the guide bar is a pair of to the upper and lower both ends of installation fixed plate are installed, and the expansion plate is installed to the telescopic cylinder expansion end, and the guide bar all wears the expansion plate, prolongs the board before the expansion plate is installed, prolongs the board about, and the guide cylinder is installed to both sides, and the guide bar all wears in the guide cylinder.
Further, the sterilization assembly comprises an annular concave part arranged on the front extension plate, an annular cover is assembled at the upper end of the annular concave part, a lamp wick is arranged on the inner wall of the annular concave part, and alcohol is arranged in the annular concave part.
Furthermore, the inoculation mechanism comprises feed screws which are installed on the upper top of the outer cover in pairs, the feed screws are all provided with moving plates, the two ends of each moving plate are respectively provided with a moving seat, the upper ends of the moving seats are respectively provided with an inclined cylinder in an inclined manner, the outer walls of the inclined cylinders are respectively provided with a telescopic screw, the telescopic screws are all provided with a telescopic moving plate, the telescopic moving plates are respectively provided with an inoculation rod, and the other ends of the inoculation rods are respectively provided with an inoculation ring;
a plurality of moving holes are formed on the top of the outer cover, and the inoculation rods penetrate through the moving holes;
round holes are formed at one position of each moving hole.
Furthermore, a plurality of assembling grooves are formed at one end of the substrate;
the dish placing unit comprises a placing table arranged on a base plate, limiting blocks are formed at the lower end of the placing table and are assembled in an assembling groove, assembling frames are arranged at the upper end of the placing table in an equally-spaced array mode, rectangular holes are formed in two sides of each assembling frame, limiting rods are arranged in the assembling frames, springs penetrate through one ends of the limiting rods, movable clamping pieces are arranged between each pair of limiting rods, fixed clamping pieces are arranged between each pair of assembling frames, a key-shaped plate is arranged at one end of the placing table, a cover plate is arranged at the other end of the key-shaped plate, a plurality of sealing bulges are arranged at the upper end of the cover plate, and when a culture dish is placed in the outer cover, the cover plate covers the lower portion of one end of the outer cover;
the movable clamping piece comprises a culture dish clamping arc plate, assembling convex plates are formed at two ends of the culture dish clamping arc plate, the assembling convex plates are assembled on the limiting rods, and the assembling convex plates penetrate through the strip holes;
the fixed clamping pieces are arranged on a plurality of fixed convex plates, and fixed arc plates are arranged between the fixed convex plates.
The technical scheme has the advantages that:
1. the dustcoat, mainly provide an aseptic environment for the inoculation of microorganism, thereby avoid the bacterial diffusion, reduce the probability that the bacterial is polluted by other bacterial, wherein in order to provide a relatively aseptic environment, consequently set up the ultraviolet lamp in the dustcoat, to the inside sterilization operation that carries out of dustcoat when not inoculating, simultaneously, in order to further improve this kind of effect, all set up the ultraviolet lamp in the outside of motion hole, thereby prevent inside other bacterial entering dustcoat, wherein in order to avoid the aseptic environment of sterilization mechanism in to the dustcoat to produce the influence, consequently, corresponding baffle has been set up to the inside one end of dustcoat.
2. The pipe placing mechanism is arranged inside the outer cover and used for placing the microorganism test tube and driving the microorganism test tube to rotate, so that the microorganism test tube is inclined, and the inoculation mechanism is convenient to inoculate. Wherein, put a tub mechanism and fix the microorganism test tube through pressing from both sides a tub subassembly and top cap down, thereby conveniently carry out the rotation of microorganism test tube, the fixed easy operation of centre gripping of microorganism test tube, it is convenient easy, when microorganism test tube centre gripping is fixed, only need can realize the rotation of fixture block through rotating the pressing plate, finally make, the one end of fixture block acts on the outer wall of second lug, thereby shorten the length of telescopic link, thereby first double-layered pipe arc board and second double-layered pipe arc board move each other in opposite directions, and finally with microorganism test tube centre gripping between first double-layered pipe arc board and second double-layered pipe arc board, when the centre gripping, in order to prevent that first double-layered pipe arc board and second double-layered pipe arc board centre gripping dynamics is too big to cause the fracture of microorganism test tube, consequently it is protruding to have set up corresponding rubber pad at first double-layered pipe arc board and second double-layered pipe arc board inner wall, on the one hand has improved the frictional force, on the other hand improves and avoids the fracture of microorganism test tube, and put tub mechanism can take place the rotation under the effect of rotating the cylinder again, thereby make microorganism test tube slope, thereby make things convenient for inoculation mechanism to carry out inoculation operation.
3. Plug pulling mechanism, a test tube stopper for extracting the microorganism test tube, wherein for the convenience realizes extracting of test tube stopper, consequently, dedicated test tube stopper has been set up, this test tube stopper is drawn the piece by the plug of locating microorganism test tube mouth of pipe department and the T shape that sets up at the plug and is constituteed, and pertinence ground, plug pulling mechanism is in plug pulling operation in-process, inward movement under the effect of centre gripping cylinder, and draw a centre gripping with the T shape, then start the lift cylinder, the lift cylinder upwards draws the test tube stopper until extracting the test tube stopper, and simultaneously, plug pulling mechanism still conveniently plugs the test tube stopper at the mouth of pipe of microorganism test tube, thereby reduce contaminated risk.
4. The sterilization mechanism, a sterilization operation for the orificial sterilization operation of microorganism test tube, of course this sterilization operation should go on after the unplugging mechanism extracts the test tube stopper, wherein, lifting unit can make the sterilization subassembly carry out elevating movement, thereby carry out sterilization operation to orificial one section length of microorganism test tube through the sterilization subassembly, and the convenience shifts out the sterilization subassembly after the sterilization is accomplished, wherein, telescopic component can make the sterilization subassembly carry out the removal of horizontal direction, when the microorganism test tube needs the sterilization, sterilization subassembly forward motion to the orificial department of microorganism test tube under telescopic cylinder's drive, and in order to improve the stability of motion, therefore corresponding guide bar has been set up, the guide bar still wears in the expansion plate, wear in the guide cylinder that sets up preceding extension plate simultaneously, the stability of extension plate forward motion has been improved through above-mentioned two parts, the vibrations of small amplitude appear when preventing preceding extension plate forward motion, thereby influence control effect, the sterilization subassembly adopts annular flame's mode to carry out sterilization operation to the orificial test tube of microorganism.
5. The inoculation mechanism shifts the bacterial in the microorganism test tube to the culture dish, and in the transfer process, drives the inoculating loop to move under the drive of the feed screw, wherein, the inclined cylinder arranged on the feed screw plays a guiding role when the inoculating rod moves in a telescopic way, and the inoculating loop is convenient to inoculate in a slant way, wherein the inoculating loop moves in a telescopic way under the drive of the telescopic screw, thereby improving the stability of the movement.
6. Put the ware unit, can fix the culture dish centre gripping on placing the platform through fixed folder and movable folder, wherein the movable folder is under the effect of spring, move towards fixed folder side, thereby with the culture dish centre gripping between fixed folder and movable folder, wherein for the convenience with the culture dish after the centre gripping is accomplished inside pushing away the dustcoat, consequently, will place the platform assembly on the base plate, inside in order to prevent that external bacterial from getting into the dustcoat, consequently corresponding key board has been set up on placing the platform, corresponding apron has been set up at the other end of key board simultaneously, when implementing the inoculation, the apron lid closes the one side at the dustcoat, thereby make the dustcoat form a comparatively inclosed aseptic environment.
7. The method improves the efficiency of microbial inoculation, can effectively prevent other microorganisms from influencing target strains, is simple to operate, can effectively reduce the possibility of infection of the target strains to operators, and has strong practicability.
Drawings
Fig. 1 shows a first perspective view of the present invention.
Fig. 2 shows a second perspective view of the present invention.
Fig. 3 shows a partial cross-sectional view one.
Fig. 4 shows a partial sectional view two.
Fig. 5 shows a partial cross-sectional view three.
Fig. 6 shows a partial cross-sectional view four.
Fig. 7 shows a partial enlarged view at a.
Fig. 8 shows a partial enlarged view at B.
Fig. 9 shows a partial enlarged view at C.
Fig. 10 shows a partial enlarged view at D.
Fig. 11 shows a partial enlarged view at E.
Fig. 12 shows a partial enlarged view at F.
Fig. 13 shows a partial enlarged view at G.
Fig. 14 shows a partial enlarged view at H.
Fig. 15 shows a partial enlarged view at M.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or the orientations or positional relationships that the products of the present invention are conventionally placed in when used, and are only used for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements to be referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
The terms "first," "second," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
The terms "parallel", "perpendicular", etc. do not require that the components be absolutely parallel or perpendicular, but may be slightly inclined. For example, "parallel" merely means that the directions are more parallel relative to "perpendicular," and does not mean that the structures are necessarily perfectly parallel, but may be slightly tilted.
Furthermore, the terms "substantially", and the like are intended to indicate that the relative terms are not necessarily strictly required, but may have some deviation. For example: "substantially equal" does not mean absolute equality, but because absolute equality is difficult to achieve in actual production and operation, certain deviations generally exist. Thus, in addition to absolute equality, "substantially equal" also includes the above-described case where there is some deviation. In this case, unless otherwise specified, terms such as "substantially", and the like are used in a similar manner to those described above.
In the description of the present invention, it should also be noted that, unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1 to 15, a microbial aseptic inoculation apparatus includes: dustcoat 1 puts a tub mechanism 2, locates in dustcoat 1 for placing and drive microorganism test tube 27 of microorganism test tube 27 rotate, so that microorganism test tube 27 slope, including locating the base plate 20 of dustcoat 1 bottom plate, base plate 20 one end is formed with assembly groove 200 a plurality ofly. The plug-pulling mechanism 3 is arranged in the outer cover 1 and is used for pulling out a test tube plug 28 of the microorganism test tube 27. And the sterilization mechanism 4 is arranged in the outer cover 1 and is used for sterilizing the pipe orifice of the microorganism test tube 27. And an inoculation mechanism 5 which is arranged at the upper end of the outer cover 1 and transfers the strains in the microorganism test tube 27 to a culture dish. And the dish placing unit 6 is arranged on the base plate 20 in the outer cover 1 and is used for placing a culture dish. The sterilization mechanism 4 and the dish placing unit 6 are respectively positioned at two ends of the outer cover 1, the pipe placing mechanism 2 is positioned between the sterilization mechanism 4 and the dish placing unit 6, the plug pulling mechanism 3 is positioned above the pipe placing mechanism 2, and the inoculation mechanism 5 moves along the length direction of the outer cover 1 and is used for inoculation of microorganisms.
Put tub mechanism 2 including being equal interval array ground and installing in the support piece 21 of base plate 20 one end, support piece 21 upper end all takes shape there is concave groove 210, all be equipped with connecting piece 23 in the concave groove 210, bull stick 22 is all installed to connecting piece 23 one end, bull stick 22 assembles in support piece 21 upper end, the one end of bull stick 22 is connected with rotation cylinder 29, rotation cylinder 29 is installed in dustcoat 1, the double-layered pipe subassembly 24 is all installed to the connecting piece 23 other end, double-layered pipe subassembly 24 is used for the fixed microorganism test tube 27 of centre gripping, lower extension pole 25 is installed to connecting piece 23 all downwardly extending, lower hood 26 is installed to lower extension pole 25 lower extreme, microorganism test tube 27 lower extreme is arranged in lower hood 26. The connecting member 23 includes a rotary block 230 fitted at the concave groove 210, and a fixing plate 231 is installed at the other end of the rotary block 230. The pipe clamping assembly 24 comprises a first pipe clamping arc plate 240 installed at the other end of the fixed connecting plate 231, first lugs 2400 are formed at two ends of the first pipe clamping arc plate 240, telescopic rods 241 penetrate through the first lugs 2400, end plugs 242 are formed at one ends of the telescopic rods 241, rotating shafts 243 are assembled at the other ends of the telescopic rods 241, rotating shafts 243 are assembled with rotating pieces 244, clamping blocks 245 are formed on outer arc walls of the rotating pieces 244, pressing plates 246 are formed on outer arc walls of the rotating pieces 244, and the pressing plates 246 are perpendicular to the clamping blocks 245. The tube clamping assembly 24 further includes a second tube clamping arc plate 247, wherein second lugs 2470 are formed at two ends of the second tube clamping arc plate 247, and the second lugs 2470 penetrate through the telescopic rod 241. When the clamping tube assembly 24 clamps the microorganism test tube 27, the rotating member 244 is rotated by the pressing plate 246 so that the other end of the clamping block 245 acts on the second protrusion 2470. Rubber pad protrusions 248 are arranged on the inner walls of the first pipe clamping arc plate 240 and the second pipe clamping arc plate 247.
Put tub mechanism 2, locate inside the dustcoat 1 for placing and drive microorganism test tube 27 of microorganism test tube 27 rotate to make microorganism test tube 27 slope, thereby make things convenient for inoculation mechanism 5 to carry out the inoculation operation. Wherein, put tub mechanism 2 and fix microorganism test tube 27 through pressing from both sides tub subassembly 24 and lower hood 26, thereby conveniently carry out microorganism test tube 27's rotation, microorganism test tube 27's centre gripping fixed operation is simple, convenient and easy, when microorganism test tube centre gripping is fixed, only need can realize the rotation of fixture block 245 through rotating pressing plate 246, finally make, the one end of fixture block 245 acts on the outer wall of second lug 2470, thereby shorten the length of telescopic link 241, thereby first double-layered pipe arc board 240 and second double-layered pipe arc board 247 move each other in opposite directions, and finally with microorganism test tube 27 centre gripping between first double-layered pipe arc board 240 and second double-layered pipe arc board 247, when the centre gripping, in order to prevent that first double-layered pipe arc board 240 and second double-layered pipe arc board 247's centre gripping dynamics is too big to cause the fracture of microorganism test tube 27, consequently set up corresponding rubber pad 248 at first double-layered pipe arc board 240 and second double-layered pipe arc board 247 inner wall, on the one hand improved the friction, avoid the fracture of microorganism test tube 27 again, and put tub 2 and make the rotation cylinder 27 of inoculation take place, thereby make the rotation of microorganism inoculation mechanism 5, thereby the rotation of the inoculation mechanism, thereby the microorganism inoculation mechanism takes place, thereby the rotation of the microorganism inoculation mechanism, thereby make the microorganism inoculation mechanism, thereby.
Plug pulling mechanism 3 is including installing in the lift cylinder 36 that pushes up on dustcoat 1, lifter plate 35 is installed to the expansion end of lift cylinder 36, lifter plate 35 both sides are installed the installation and are prolonged board 37 a plurality ofly, the installation is prolonged the board 37 other end and all installs dead lever 34 with downwardly extending, the dead lever 34 that is located the homonymy all installs clamping assembly plate 30, clamping assembly plate 30 all takes shape symmetrically has pilot hole 300, pilot hole 300 all is equipped with grip block 31, grip block 31 is L shape structure, grip block 32 is all installed to grip block 31, cylinder mounting panel 33 is installed to the grip block 32 other end, cylinder mounting panel 33 is all installed in clamping assembly plate 30.
The plug pulling mechanism 3 is used for pulling out the test tube plug 28 of the microbial test tube 27, wherein the plug pulling mechanism 28 is convenient to pull out the test tube plug 28, so that a special test tube plug 28 is provided, the test tube plug 28 is composed of a rubber plug arranged at the tube opening of the microbial test tube 27 and a T-shaped pulling piece 281 arranged on the rubber plug 280, pertinence is achieved, the plug pulling mechanism 3 moves inwards under the action of the clamping cylinder 32 in the plug pulling operation process, the T-shaped pulling piece 281 is clamped, then the lifting cylinder 36 is started, the lifting cylinder 36 upwards pulls out the test tube plug 28 until the test tube plug 28 is pulled out, and meanwhile, the plug pulling mechanism 3 is further convenient to plug the test tube plug 28 at the tube opening of the microbial test tube 27, so that the risk of pollution is reduced.
The sterilization mechanism 4 is including locating the lifting unit 40 a plurality of base plate 20 other end, and lifting unit 40 all is equipped with telescopic component 41, and sterilization subassembly 42 is all installed to the telescopic component 41 other end. The lifting assembly 40 comprises a plurality of extension mounting plates 400 mounted at one end of the substrate 20, and a lifting screw 401 is mounted at the other end of each extension mounting plate 400 in an upward extending manner. A partition plate 10 is arranged in the housing 1, a plurality of through holes 100 are formed in the partition plate 10, and the telescopic assembly 41 penetrates through the through holes 100. The telescopic assembly 41 comprises a lifting assembly plate 403 assembled on a lifting screw 401, an installation fixing plate 410 is installed at one end of the lifting assembly plate 403, a telescopic cylinder 411 is installed on the installation fixing plate 410, a pair of guide rods 412 are installed at the upper end and the lower end of the installation fixing plate 410, a telescopic plate 413 is installed at the movable end of the telescopic cylinder 411, the guide rods 412 penetrate through the telescopic plate 413, a front extension plate 414 is installed on the telescopic plate 413, guide cylinders 415 are installed on the upper side and the lower side of the front extension plate 414, and the guide rods 412 penetrate through the guide cylinders 415. The sterilization assembly 42 comprises an annular concave member 420 arranged on the front extension plate 414, an annular cover 421 is arranged at the upper end of the annular concave member 420, a wick 422 is arranged on the inner wall of the annular concave member 420, and alcohol is arranged in the annular concave member 420.
The sterilization mechanism 4 is used for sterilization operation of the nozzle of the microorganism test tube 27, which should be performed after the plug pulling mechanism 3 pulls out the test tube plug 28, wherein the lifting assembly 40 can make the sterilization assembly 42 perform lifting movement, so that the sterilization operation is performed on a section of the nozzle of the microorganism test tube 27 through the sterilization assembly 42, and it is convenient to move the sterilization assembly 42 out after the sterilization is completed, wherein the telescopic assembly 41 can make the sterilization assembly 42 perform horizontal movement, when the microorganism test tube 27 needs to be sterilized, the sterilization assembly 42 moves forward to the nozzle of the microorganism test tube 27 under the driving of the telescopic cylinder 411, and in order to improve the stability of the movement, a corresponding guide rod 412 is provided, the guide rod 412 also penetrates through the telescopic plate 413 and simultaneously penetrates through a guide cylinder 415 arranged in the front extension plate 414, the stability of the front extension movement of the front extension plate 414 is improved through the above two components, small amplitude vibration is prevented when the front extension plate 414 moves forward, so that the control effect is influenced, and the sterilization operation is performed on the nozzle of the microorganism test tube 27 by the sterilization assembly 42 in a ring flame mode.
The dish placing unit 6 can clamp and fix a culture dish on the placing platform 60 through the fixed clamping piece 65 and the movable clamping piece 64, wherein the movable clamping piece 64 moves towards the fixed clamping piece 65 under the action of the spring 63, so as to clamp the culture dish between the fixed clamping piece 65 and the movable clamping piece 64, in order to conveniently push the clamped culture dish to the inside of the housing 1, the placing platform 60 is assembled on the base plate 20, in order to prevent external strains from entering the inside of the housing 1, a corresponding key plate 61 is arranged on the placing platform 60, meanwhile, a corresponding cover plate 66 is arranged at the other end of the key plate 61, and when inoculation is carried out, the cover plate 66 covers one side of the housing 1, so that the housing 1 forms a relatively closed sterile environment.
The specific implementation mode is as follows:
in the specific implementation process, the ultraviolet lamps are electrified to sterilize the inside of the outer cover 1, and after the sterilization is finished, the inoculation process is started.
Before the inoculation process, firstly fixing three target microorganism test tubes 27 on the clamping tube assembly 24 of the tube placing mechanism 2, placing the lower ends of the microorganism test tubes 27 on the lower top cap 26, rotating the pressing plate 246 of the clamping tube assembly 24 to enable the clamping blocks 245 to act on the second lugs 2470, so that the clamping tube assembly 24 effectively clamps the microorganism test tubes 27, after clamping is completed, starting the lifting cylinder 36 of the plug pulling mechanism 3 to enable the clamping plate 31 to move downwards until the clamping plate 31 moves to the T-shaped drawing part 281, then starting the clamping cylinder 32 to enable the clamping plate 31 to clamp the T-shaped drawing part 281, then enabling the lifting cylinder 36 to move upwards to pull the test tube plugs 28 out of the microorganism test tubes 27, after completion, starting the telescopic cylinder 411 to push the ignited sterilization assembly 42 to the microorganism test tubes 27 until the sterilization assembly 42 is sleeved at the tube mouths of the microorganism test tubes 27, then, the lifting screw 401 is started to sterilize the sterilization assembly 42 at high temperature, after sterilization is completed, the sterilization assembly 42 is moved out of the microorganism test tube 27 through the lifting screw 401 and the telescopic cylinder 411, then the side cover of the housing 1 is covered, after covering, inoculation operation is started, before inoculation, the microorganism test tube 27 needs to be rotated through the rotating cylinder 29, the rotating angle is generally 45 degrees, after inclination is completed, the inoculating loop 57 is moved to the tube orifice of the microorganism test tube 27 through the feed screw 50, then the telescopic screw 54 is started to move the inoculating loop 57 into the bacterial liquid of the microorganism test tube 27, after completion, the telescopic screw 54 is started, the inoculating loop 57 moves out of the microorganism test tube 27, then the feed screw 50 is started, the inoculating loop 57 moves out of the culture dish, and slides for a certain distance in the culture dish until inoculation is completed. Before inoculation, the culture dish is required to be placed between the movable clamping piece 64 and the fixed clamping piece 65, the culture dish is clamped, after the culture dish is clamped, the placing table 60 is pushed inwards, the placing table 60 is located inside the inner cover 1, and after the inoculation is completed, multiple times of inoculation can be carried out on different culture dishes according to needs.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and it is apparent that those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (1)
1. A sterile microbial inoculation apparatus, comprising:
an outer cover (1) is arranged on the outer side of the shell,
the tube placing mechanism (2) is arranged in the outer cover (1) and used for placing the microorganism test tube (27) and driving the microorganism test tube (27) to rotate so as to enable the microorganism test tube (27) to incline, and comprises a base plate (20) arranged on the bottom plate of the outer cover (1);
the plug pulling mechanism (3) is arranged in the outer cover (1) and is used for pulling out a test tube plug (28) of the microorganism test tube (27);
the sterilization mechanism (4) is arranged in the outer cover (1) and is used for sterilizing the pipe orifice of the microorganism test tube (27);
the inoculation mechanism (5) is arranged at the upper end of the outer cover (1) and is used for transferring strains in the microorganism test tube (27) to a culture dish;
the dish placing unit (6) is arranged on the substrate (20) in the outer cover (1) and is used for placing a culture dish;
the sterilization mechanism (4) and the dish placing unit (6) are respectively positioned at two ends of the outer cover (1), the pipe placing mechanism (2) is positioned between the sterilization mechanism (4) and the dish placing unit (6), the plug pulling mechanism (3) is positioned above the pipe placing mechanism (2), and the inoculation mechanism (5) can move along the length direction of the outer cover (1) and is used for inoculation of microorganisms;
the tube placing mechanism (2) comprises support pieces (21) which are arranged at one end of a base plate (20) in an equally-spaced array mode, concave grooves (210) are formed in the upper ends of the support pieces (21), connecting pieces (23) are assembled in the concave grooves (210), rotating rods (22) are installed at one ends of the connecting pieces (23), the rotating rods (22) are assembled at the upper ends of the support pieces (21), one ends of the rotating rods (22) are connected with rotating cylinders (29), the rotating cylinders (29) are installed in an outer cover (1), tube clamping assemblies (24) are installed at the other ends of the connecting pieces (23), the tube clamping assemblies (24) are used for clamping and fixing microorganism test tubes (27), lower extension rods (25) are installed on the connecting pieces (23) in a downward extending mode, lower top caps (26) are installed at the lower ends of the lower extension rods (25), and the lower ends of the microorganism test tubes (27) are placed in the lower top caps (26);
the connecting piece (23) comprises a rotating block (230) assembled at the concave groove (210), and the other end of the rotating block (230) is provided with a fixed connecting plate (231);
the pipe clamping assembly (24) comprises a first pipe clamping arc plate (240) arranged at the other end of the fixed connecting plate (231), first lugs (2400) are formed at two ends of the first pipe clamping arc plate (240), telescopic rods (241) penetrate through the first lugs (2400), an end plug (242) is formed at one end of each telescopic rod (241), rotating shafts (243) are assembled at the other ends of the telescopic rods (241), rotating members (244) are assembled on the rotating shafts (243), clamping blocks (245) are formed on the outer arc wall of each rotating member (244), pressing plates (246) are further formed on the outer arc wall of each rotating member (244), and the pressing plates (246) are perpendicular to the clamping blocks (245);
the pipe clamping assembly (24) further comprises a second pipe clamping arc plate (247), second lugs (2470) are formed at two ends of the second pipe clamping arc plate (247), and the second lugs (2470) penetrate through the telescopic rod (241);
when the clamping tube assembly (24) clamps and fixes the microorganism test tube (27), the rotating piece (244) rotates under the action of the pressing plate (246) so that the other end of the clamping block (245) acts on the second lug (2470);
rubber pad bulges (248) are arranged on the inner walls of the first pipe clamping arc plate (240) and the second pipe clamping arc plate (247);
the plug pulling mechanism (3) comprises a lifting cylinder (36) arranged on the upper top of the outer cover (1), a lifting plate (35) is arranged at the movable end of the lifting cylinder (36), a plurality of installation extension plates (37) are arranged on two sides of the lifting plate (35), fixing rods (34) are arranged at the other ends of the installation extension plates (37) in a downward extending mode, clamping assembly plates (30) are arranged on the fixing rods (34) on the same side, assembly holes (300) are symmetrically formed in the clamping assembly plates (30), clamping plates (31) are arranged in the assembly holes (300), the clamping plates (31) are of an L-shaped structure, clamping cylinders (32) are arranged on the clamping plates (31), cylinder mounting plates (33) are arranged at the other ends of the clamping cylinders (32), and the cylinder mounting plates (33) are arranged on the clamping assembly plates (30);
the sterilization mechanism (4) comprises a plurality of lifting components (40) arranged at the other end of the substrate (20), the lifting components (40) are all provided with telescopic components (41), and the other ends of the telescopic components (41) are all provided with sterilization components (42);
the lifting assembly (40) comprises a plurality of extension mounting plates (400) arranged at one end of the base plate (20), and lifting screw rods (401) are arranged at the other ends of the extension mounting plates (400) in an upward extending manner;
a partition plate (10) is arranged in the outer cover (1), a plurality of through holes (100) are formed in the partition plate (10), and the telescopic assembly (41) penetrates through the through holes (100);
the telescopic assembly (41) comprises a lifting assembly plate (403) assembled on a lifting screw (401), one end of the lifting assembly plate (403) is provided with an installation fixing plate (410), the installation fixing plate (410) is provided with a telescopic cylinder (411), the upper end and the lower end of the installation fixing plate (410) are provided with a pair of guide rods (412), the movable end of the telescopic cylinder (411) is provided with a telescopic plate (413), the guide rods (412) penetrate through the telescopic plate (413), the telescopic plate (413) is provided with a front extension plate (414), the upper side and the lower side of the front extension plate (414) are provided with guide cylinders (415), and the guide rods (412) penetrate through the guide cylinders (415);
the sterilizing component (42) comprises an annular concave part (420) arranged on the front extension plate (414), an annular cover (421) is assembled at the upper end of the annular concave part (420), a lamp wick (422) is arranged on the inner wall of the annular concave part (420), and alcohol is arranged in the annular concave part (420);
the inoculation mechanism (5) comprises feed screws (50) which are installed on the upper top of the outer cover (1) in pairs, the feed screws (50) are all provided with a moving plate (51), two ends of the moving plate (51) are both provided with a moving seat (52), the upper ends of the moving seats (52) are all obliquely provided with an inclined cylinder (53), the outer wall of the inclined cylinder (53) is both provided with a telescopic lead screw (54), the telescopic lead screws (54) are both provided with a telescopic moving plate (55), the telescopic moving plates (55) are both provided with inoculation rods (56), and the other ends of the inoculation rods (56) are both formed with inoculation rings (57);
a plurality of moving holes (11) are formed on the upper top of the outer cover (1), and the inoculation rods (56) penetrate through the moving holes (11);
round holes (12) are formed at one position of each motion hole (11);
one end of the substrate (20) is provided with a plurality of assembling grooves (200);
the dish placing unit (6) comprises a placing table (60) arranged on a substrate (20), a limiting block (600) is formed at the lower end of the placing table (60), the limiting blocks (600) are all assembled in an assembling groove (200), assembling frames (62) are arranged at the upper end of the placing table (60) in an equally-spaced array mode, long-strip-shaped holes (620) are formed in the two sides of each assembling frame (62), limiting rods are arranged in the assembling frames (62), springs (63) penetrate through one ends of the limiting rods, movable clamping pieces (64) are arranged between each pair of limiting rods, fixed clamping pieces (65) are arranged between each pair of assembling frames (62), a key-shaped plate (61) is arranged at one end of the placing table (60), a cover plate (66) is arranged at the other end of the key-shaped plate (61), sealing bulges (660) are arranged at the upper end of the cover plate (66) in a plurality of the sealing bulges (660), and when a culture dish is placed in the outer cover (1), the cover plate (66) covers the lower portion of one end of the outer cover plate (1);
the movable clamp (64) comprises a culture dish clamping arc plate (641), assembling convex plates (640) are formed at two ends of the culture dish clamping arc plate (641), the assembling convex plates (640) are assembled on the limiting rods, and the assembling convex plates (640) penetrate through the strip holes (620);
the fixed clamping pieces (65) are all arranged on a plurality of fixed convex plates (651), and fixed arc plates (650) are arranged between the fixed convex plates (651).
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CN202010276751.1A CN111423976B (en) | 2020-04-10 | 2020-04-10 | Aseptic inoculation device of microorganism |
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