CN106906140B - A kind of strain screening, cultural method and its complexes - Google Patents
A kind of strain screening, cultural method and its complexes Download PDFInfo
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- CN106906140B CN106906140B CN201710110789.XA CN201710110789A CN106906140B CN 106906140 B CN106906140 B CN 106906140B CN 201710110789 A CN201710110789 A CN 201710110789A CN 106906140 B CN106906140 B CN 106906140B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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Abstract
The present invention provides a kind of Spawn incubation method and its complexes, belong to bioengineering field.This kind of Spawn incubation method, which is characterized in that comprise the steps of: a, sterile water is boiled, brown sugar is added, brown sugar is melted out;B, peptone, potassium dihydrogen phosphate and vitamin is added to sterile water, heats bacterium of removing the evil and culture medium is made;B, strain, after being sufficiently stirred, stationary culture 1-2 days is added to 20-35 DEG C in cooling culture medium;C, step b is put in complexes and is continued shake culture 5-8 days, complexes, including ontology offer place position in ontology, shaking flask turntable is provided in ontology place position.The present invention has the advantages that more easily cultivate strain.
Description
Technical field
The invention belongs to bioengineering field, it is related to a kind of strain screening, cultural method and its complexes.
Background technique
The method that biological inoculum culture mainly uses microorganism or enzyme, and be that carrier will be therein by suitable culture medium
Low value substance is converted into high added value, high bioactivity or flavor substance etc..It is a large amount of to have during bioconversion
Bacterial strain and the cell strain etc. for having related enzyme activity or other biological activities all have to pass through shaking flask culture, to screen high-performance strain.
Currently, concussion movement is single during existing strain shake culture, and it is above-mentioned due to there is a problem of, through retrieving,
As Chinese patent literature disclose it is a kind of in microorganism shaking table culture bottle pallet and bottle holder [patent No.:
ZL201520853419.1;Authorization Notice No.: CN205152203U].This culture bottle pallet and bottle for microorganism shaking table
Support, it includes shaking table outer housing, and shaking table outer housing bottom is equipped with crank and rocker mechanism, and motor drives crank by driving belt
Rocker device drives shaking table frame entirety to swing, and the flexible strand that shaking table frame is arranged symmetrically by more is fixedly mounted on outside shaking table
The top of shell, the bottom of shaking table outer housing are equipped with lower margin idler wheel, and shaking table lower portion is provided with multiple article putting grooves, article putting groove
On be placed with culture bottle pallet, bottle holder is mounted on culture bottle pallet, is nested with culture bottle on bottle holder.
But the culture bottle pallet of this microorganism shaking table and the structure of bottle holder are simple, transmission stability is poor, so, for
Those skilled in the art, needing to be developed one kind being capable of more stable Spawn incubation shaking table.
Summary of the invention
The first purpose of this invention is the presence of the above problem in view of the prior art, proposes a kind of Spawn incubation side
Method, this kind of Spawn incubation method have the characteristics that versatile, simple.
The first purpose of this invention can be realized by the following technical scheme:
A kind of strain screening, cultural method, which is characterized in that comprise the steps of:
A, sterile water is boiled, brown sugar is added, brown sugar is melted out;
B, peptone, potassium dihydrogen phosphate and vitamin is added to sterile water, heats bacterium of removing the evil and culture medium is made;
B, strain, after being sufficiently stirred, stationary culture 1-2 days is added to 20-35 DEG C in cooling culture medium;
C, step b is put in complexes and is continued shake culture 5-8 days.
Second object of the present invention is the presence of the above problem in view of the prior art, proposes a kind of complexes, should
Complexes, which have the characteristics that more easily to realize, cultivates strain.
Second object of the present invention can be realized by the following technical scheme:
A kind of complexes, which is characterized in that including ontology, which is characterized in that place position is offered in the ontology,
It is provided with shaking flask turntable in the ontology place position, a fixed plate is horizontally fixed in the ontology place position, it is described
The opposite two sides of fixed plate are respectively fixed with auricle one and auricle two, and the auricle one and auricle two are fixed by screw respectively
Through-hole one is also provided on place position inner wall, in fixed plate, the upper surface of fixed plate is vertically fixed several adjustment springs, institute
It is also horizontally disposed between the shaking flask turntable stated and fixed plate to have an adjustable plate, through-hole two is offered on the adjustable plate, it is described
The other end of adjustment spring be each attached to the lower surface of adjustable plate, there are also a mounting plate, the peaces for setting below fixed plate
The lower surface of loading board is further fixed on driving motor, the output shaft of the driving motor straight up, and the output of driving motor
Shaft end portion is horizontally disposed a connecting rod, and the other end of the connecting rod is vertically arranged with a drive shaft, and the drive shaft is pierced by
Through-hole one in fixed plate, and the end of drive shaft is fixed on the lower surface of shaking flask turntable;Energy is fixed on the mounting plate
The swing mechanism for enough shaking flask turntable being driven to swing up and down.
Strain to be cultivated is placed in shaking flask, and shaking flask is placed on shaking flask turntable, driving motor drives connecting rod
Rotation, so as to drive drive shaft to be rotated centered on driving motor output shaft, and the other end fixation of drive shaft is shaken
On bottle turntable, the shaking flask being able to drive on shaking flask turntable is circumferentially rotated centered on driving motor output shaft;Meanwhile passing through
Swing mechanism is able to drive mounting plate and is swung above and below, so that the driving motor on mounting plate is driven to swing above and below, shaking flask
Turntable, by the adjustment spring between adjustable plate and fixed plate, can play certain buffer function during swinging up and down,
To be able to achieve the upper and lower stable oscillation of shaking flask, to make strain and culture solution, air in shaking flask come into full contact with, more favorably
In Spawn incubation.
In above-mentioned complexes, the upper surface of the shaking flask turntable is fixed with circular column one and circular column two, described
Circular column one be located in circular column two, be circumferentially equidistantly fixed with several adjustable springs on the outer wall of the circular column one
One, the other end of the adjustable spring one is vertically fixed intermediate plate one, and the upper surface of the shaking flask turntable is also solid vertically
Surely there are several intermediate plates two to match with intermediate plate one, the intermediate plate two is fixed with flexible bullet on an arc surface of intermediate plate one
Spring two, the other end of the adjustable spring two are fixed with intermediate plate three, and the upper surface of the shaking flask turntable is also vertically arranged with
Several and matched intermediate plate four of intermediate plate three-phase, the intermediate plate one, intermediate plate two, intermediate plate three and intermediate plate four are respectively positioned on circular column two
It is interior, and adjustable spring three is provided between two inner wall of intermediate plate four and circular column.
Shaking flask can be clamped between corresponding intermediate plate one and intermediate plate two, and wherein intermediate plate two is fixed on shaking flask turntable
For fixing end, and clamp clip one can it is mobile relative to shaking flask turntable be movable end, can be according to shaking flask ruler by adjustable spring one
The very little spacing adjusted between intermediate plate one and intermediate plate two;In addition, shaking flask can be clamped at corresponding intermediate plate three and intermediate plate four it
Between, wherein intermediate plate three and intermediate plate four can be movable end relative to the movement of shaking flask turntable, and intermediate plate three can be using intermediate plate two as branch
Support point can adjust the spacing between intermediate plate three and intermediate plate four by adjustable spring two and adjustable spring three according to shaking flask size,
And adjustable range is wider than the range between intermediate plate one and intermediate plate two.
In above-mentioned complexes, the swing mechanism include the bottom wall upper cylinder one for being fixed on place position, cylinder two,
Cylinder three and cylinder four, the cylinder one, cylinder two, cylinder three and cylinder four piston rod straight up, under mounting plate
Surface is fixed on the piston rod end of cylinder one, cylinder two, cylinder three and cylinder four.Cylinder one, cylinder two, cylinder three and cylinder
Four are distributed on four angles of mounting plate, and drive mounting plate that can be more stable carries out horizontal upper and lower swing, to be able to achieve
The upper and lower swing of shaking flask.
In above-mentioned complexes, the through-hole two on the adjustable plate is equal with the aperture of through-hole one in fixed plate,
The connecting rod obtains the radius that length is less than fixed plate through-hole one.
In above-mentioned complexes, electric heating tube is provided in the one side wall of the ontology place position.Electric heating tube energy
Heat is distributed, enough so as to adjust the temperature in place position according to the actual conditions of Spawn incubation.
In above-mentioned complexes, headlamp is provided on the roof of the ontology place position.Headlamp is capable of providing
Light source, so as to control in place position whether need illumination according to the actual conditions of Spawn incubation.
In above-mentioned complexes, sliding in fluted, described groove is opened up on a side wall of the ontology and is provided with
It pushes, is respectively corresponded on the roof and bottom wall of the ontology place position and offer sliding slot one and sliding slot two.Pushing can be recessed
It promotes, release in slot, and the upper and lower end that pushes is slided in sliding slot one and sliding slot two, so as to be closed during Spawn incubation,
To avoid external interference.
In above-mentioned complexes, control panel and alarm lamp, the driving motor, gas are provided on the ontology
Cylinder one, cylinder two, cylinder three, four alarm lamp of cylinder, electric heating tube and headlamp pass through route and are connected with control panel.
In above-mentioned complexes, the lower surface of the ontology is provided with several universal wheels.Pass through universal wheel, Neng Goubian
It is moved in ontology.
Compared with prior art, the screening of this strain, cultural method and its complexes have the advantage that
1, the Spawn incubation method in the present invention is simple and convenient, and stability is good.
2, the driving motor in the present invention is able to drive the shaking flask on shaking flask turntable by connecting rod and drive shaft to drive electricity
It is circumferentially rotated centered on machine output shaft;Meanwhile cylinder one, cylinder two, cylinder three and cylinder four can be more stable band
Dynamic mounting plate is swung above and below, so that the upper and lower swing of shaking flask is able to achieve, to make the strain and culture solution, sky in shaking flask
Gas comes into full contact with, and is more advantageous to Spawn incubation.
3, the present invention can be clamped between corresponding intermediate plate one and intermediate plate two by shaking flask, and wherein intermediate plate two is fixed
It is fixing end on shaking flask turntable, and clamp clip one can be movable end relative to the movement of shaking flask turntable, pass through one energy of adjustable spring
Enough adjust the spacing between intermediate plate one and intermediate plate two;Shaking flask can also be clamped between corresponding intermediate plate three and intermediate plate four,
Intermediate plate three and intermediate plate four can be movable end relative to the movement of shaking flask turntable, and intermediate plate three can be led to using intermediate plate two as supporting point
It crosses adjustable spring two and adjustable spring three can adjust spacing between intermediate plate three and intermediate plate four, and adjustable range is than one He of intermediate plate
Range between intermediate plate two is wider.
Detailed description of the invention
Fig. 1 is schematic perspective view of the invention;
Fig. 2 is schematic cross-sectional view one of the invention;
Fig. 3 is schematic cross-sectional view two of the invention;
Fig. 4 is the partial enlargement structural representation at A of the invention.
In figure, 1, ontology;2, place position;3, shaking flask turntable;4, fixed plate;5, auricle one;6, auricle two;7, mounting plate;
8, driving motor;9, connecting rod;10, drive shaft;11, circular column one;12, circular column two;13, adjustable spring one;14, intermediate plate one;
15, intermediate plate two;16, adjustable spring two;17, intermediate plate three;18, intermediate plate four;19, adjustable spring three;20, cylinder one;21, cylinder
Two;22, cylinder three;23, cylinder four;24, adjustment spring;25, adjustable plate;26, electric heating tube;27, headlamp;28, it pushes;
29, alarm lamp;30, control panel;31, universal wheel.
Specific embodiment
Following is a specific embodiment of the present invention in conjunction with the accompanying drawings, technical scheme of the present invention will be further described,
However, the present invention is not limited to these examples.
A kind of strain screening, cultural method, which is characterized in that comprise the steps of:
A, sterile water is boiled, brown sugar is added, brown sugar is melted out;
B, peptone, potassium dihydrogen phosphate and vitamin is added to sterile water, heats bacterium of removing the evil and culture medium is made;
B, strain, after being sufficiently stirred, stationary culture 1-2 days is added to 20-35 DEG C in cooling culture medium;
C, step b is put in complexes and is continued shake culture 5-8 days.
As shown in Figure 1, a kind of Spawn incubation shaking table, including ontology 1, place position 2,1 place position of ontology are offered in ontology 1
Shaking flask turntable 3 is provided in 2, the lower surface of ontology 1 is provided with several universal wheels 31, can be convenient for this by universal wheel 31
Body 1 is moved.Sliding in fluted, described groove is opened up on one side wall of ontology 1 be provided with push 28,1 place position 2 of ontology
Roof and bottom wall on respectively correspond and offer sliding slot one and sliding slot two.Pushing 28 can promote in groove, release, and push
28 upper and lower ends are slided in sliding slot one and sliding slot two, so as to be closed during Spawn incubation, to avoid external interference.
As shown in Figures 2 and 3, a fixed plate 4, the opposite two sides of fixed plate 4 point are horizontally fixed in 1 place position 2 of ontology
It is not fixed with auricle 1 and auricle 26, auricle 1 and auricle 26 are fixed by screws in respectively on 2 inner wall of place position, fixed
Offer through-hole one on plate 4, the upper surface of fixed plate 4 is vertically fixed several adjustment springs 24, the shaking flask turntable 3 and solid
It is also horizontally disposed between fixed board 4 to have an adjustable plate 25, through-hole two is offered on adjustable plate 25, the other end of adjustment spring 24 is solid
It is scheduled on the lower surface of adjustable plate 25.Shaking flask turntable 3 passes through the tune between adjustable plate 25 and fixed plate 4 during swinging up and down
Spring 24 is saved, certain buffer function can be played.
In addition, being provided with a mounting plate 7 below fixed plate 4, the lower surface of mounting plate 7 is further fixed on driving motor 8, driving
Straight up, and the output shaft end of driving motor 8 horizontally disposed has a connecting rod 9, the other end of connecting rod 9 to the output shaft of motor 8
It is vertically arranged with a drive shaft 10, the drive shaft 10 is pierced by the through-hole one in fixed plate 4, and the end of drive shaft 10 is fixed
In the lower surface of shaking flask turntable 3;
It is fixed on mounting plate 7 and is able to drive the swing mechanism that shaking flask turntable 3 swings up and down, swing mechanism includes solid
It is scheduled on bottom wall upper cylinder 1, cylinder 2 21, cylinder 3 22 and the cylinder 4 23 of place position 2, the cylinder 1, cylinder two
21, the piston rod of cylinder 3 22 and cylinder 4 23 straight up, the lower surface of mounting plate 7 be fixed on cylinder 1, cylinder 2 21,
The piston rod end of cylinder 3 22 and cylinder 4 23.Cylinder 1, cylinder 2 21, cylinder 3 22 and cylinder 4 23 are distributed in installation
On four angles of plate 7, drive mounting plate 7 that can be more stable carry out it is horizontal it is upper and lower swing, thus be able to achieve shaking flask it is upper,
Lower swing.
As shown in figure 4, the upper surface of shaking flask turntable 3 is fixed with circular column 1 and circular column 2 12, circular column one 11
In in circular column 2 12, several adjustable springs 1, adjustable spring one are circumferentially equidistantly fixed on the outer wall of circular column 1
13 other end is vertically fixed intermediate plate 1, and the upper surface of shaking flask turntable 3 is also vertically fixed several with one 14 phase of intermediate plate
Matched intermediate plate 2 15, intermediate plate 2 15 are fixed with adjustable spring 2 16, adjustable spring two on an arc surface of intermediate plate 1
16 other end is fixed with intermediate plate 3 17, and the upper surface of shaking flask turntable 3 is also vertically arranged with what several and intermediate plate 3 17 matched
Intermediate plate 4 18, intermediate plate 1, intermediate plate 2 15, intermediate plate 3 17 and intermediate plate 4 18 are respectively positioned in circular column 2 12, and intermediate plate 4 18 with
Adjustable spring 3 19 is provided between 2 12 inner wall of circular column.
Shaking flask can be clamped between corresponding intermediate plate 1 and intermediate plate 2 15, and wherein intermediate plate 2 15 is fixed on shaking flask
It is fixing end on turntable 3, and clamp clip one can be movable end relative to the movement of shaking flask turntable 3, it can by adjustable spring 1
The spacing between intermediate plate 1 and intermediate plate 2 15 is adjusted according to shaking flask size;In addition, shaking flask can be clamped at corresponding folder
Between piece 3 17 and intermediate plate 4 18, wherein intermediate plate 3 17 and intermediate plate 4 18 can be movable end relative to the movement of shaking flask turntable 3, and press from both sides
Piece 3 17 can be using intermediate plate 2 15 as supporting point, can be according to shaking flask size by adjustable spring 2 16 and adjustable spring 3 19
The spacing between intermediate plate 3 17 and intermediate plate 4 18 is adjusted, and adjustable range is more than the range between intermediate plate 1 and intermediate plate 2 15
Extensively.
Through-hole two on adjustable plate 25 is equal with the aperture of through-hole one in fixed plate 4, and the connecting rod 9 obtains length and is less than
The radius of 4 through-hole one of fixed plate.
It is provided with electric heating tube 26 in the one side wall of 1 place position 2 of ontology, electric heating tube 26 can distribute heat, so as to
Enough temperature adjusted according to the actual conditions of Spawn incubation in place position 2.Headlamp is provided on the roof of 1 place position 2 of ontology
27, headlamp 27 is capable of providing light source, so as to control in place position 2 whether need light according to the actual conditions of Spawn incubation
According to.Control panel 30 and alarm lamp 29, driving motor 8, cylinder 1, cylinder 2 21, cylinder 3 22, gas are provided on ontology 1
4 23 alarm lamp 29 of cylinder, electric heating tube 26 and headlamp 27 are connected by route with control panel 30.
Strain to be cultivated is placed in shaking flask, and shaking flask is placed on shaking flask turntable 3, driving motor 8, which drives, to be connected
Bar 9 rotates, and so as to drive drive shaft 10 to be rotated centered on 8 output shaft of driving motor, and drive shaft 10 is another
It holds on fixed shaking flask turntable 3, is able to drive the shaking flask on shaking flask turntable 3 and carries out circumferential turn centered on 8 output shaft of driving motor
It is dynamic.
Meanwhile meanwhile, the drive installation that cylinder 1, cylinder 2 21, cylinder 3 22 and cylinder 4 23 can be more stable
Plate 7 is swung above and below, to drive the driving motor 8 on mounting plate 7 to swing above and below, to be able to achieve the upper and lower of shaking flask
It swings, so that strain and culture solution, air in shaking flask be made to come into full contact with, is more advantageous to Spawn incubation.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention
The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method
In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.
Although 1, ontology is used more herein;2, place position;3, shaking flask turntable;4, fixed plate;5, auricle one;6, ear
Piece two;7, mounting plate;8, driving motor;9, connecting rod;10, drive shaft;11, circular column one;12, circular column two;13, adjustable spring
One;14, intermediate plate one;15, intermediate plate two;16, adjustable spring two;17, intermediate plate three;18, intermediate plate four;19, adjustable spring three;20, gas
Cylinder one;21, cylinder two;22, cylinder three;23, cylinder four;24, adjustment spring;25, adjustable plate;26, electric heating tube;27, it illuminates
Lamp;28, it pushes;29, alarm lamp;30, control panel;31, the terms such as universal wheel, but the possibility using other terms is not precluded
Property.The use of these items is only for be more convenient to describe and explain essence of the invention;It is construed as any one
Additional limitation is disagreed with spirit of that invention.
Claims (1)
1. a kind of Spawn incubation method, which is characterized in that comprise the steps of:
A, sterile water is boiled, brown sugar is added, brown sugar is melted out;
B, peptone, potassium dihydrogen phosphate and vitamin is added to sterile water, heats bacterium of removing the evil and culture medium is made;
C, strain, after being sufficiently stirred, stationary culture 1-2 days is added to 20-35 DEG C in cooling culture medium;
D, step c is put in complexes and is continued shake culture 5-8 days;The complexes, including ontology, the ontology
Place position is inside offered, shaking flask turntable is provided in the ontology place position, is horizontally fixed in the ontology place position
One fixed plate, the opposite two sides of the fixed plate are respectively fixed with auricle one and auricle two, the auricle one and auricle two
It is fixed by screws on place position inner wall respectively, through-hole one is also provided in fixed plate, the upper surface of fixed plate is fixed vertically
There are several adjustment springs, being also horizontally disposed between the shaking flask turntable and fixed plate has an adjustable plate, on the adjustable plate
Through-hole two is offered, the other end of the adjustment spring is each attached to the lower surface of adjustable plate, is arranged also below fixed plate
One mounting plate, the lower surface of the mounting plate are further fixed on driving motor, the output shaft of the driving motor straight up,
And driving motor output shaft end it is horizontally disposed have a connecting rod, the other end of the connecting rod is vertically arranged with a drive shaft,
The drive shaft is pierced by the through-hole one in fixed plate, and the end of drive shaft is fixed on the lower surface of shaking flask turntable;Described
It is fixed on mounting plate and is able to drive the swing mechanism that shaking flask turntable swings up and down, the upper surface of the shaking flask turntable is solid
Surely there are circular column one and circular column two, the circular column one is located in circular column two, edge on the outer wall of the circular column one
Circumferentially equidistant to be fixed with several adjustable springs one, the other end of the adjustable spring one is vertically fixed intermediate plate one, described
The upper surface of shaking flask turntable be also vertically fixed several intermediate plates two to match with intermediate plate one, the intermediate plate two is backwards to intermediate plate
Adjustable spring two is fixed on one arc surface, the other end of the adjustable spring two is fixed with intermediate plate three, and described shakes
The upper surface of bottle turntable is also vertically arranged with several and matched intermediate plate four of intermediate plate three-phase, the intermediate plate one, intermediate plate two, intermediate plate
Three and intermediate plate four be respectively positioned in circular column two, and be provided with adjustable spring three between two inner wall of intermediate plate four and circular column, it is described
Swing mechanism include the bottom wall upper cylinder one for being fixed on place position, cylinder two, cylinder three and cylinder four, the cylinder one,
Straight up, the lower surface of mounting plate is fixed on cylinder one, cylinder two, cylinder to the piston rod of cylinder two, cylinder three and cylinder four
Three and cylinder four piston rod end, the through-hole two on the adjustable plate is equal with the aperture of through-hole one in fixed plate, institute
The connecting rod stated obtains the radius that length is less than fixed plate through-hole one, is provided with electric heating in the one side wall of the ontology place position
Pipe is provided with headlamp on the roof of the ontology place position, opens up fluted on a side wall of the ontology, and described is recessed
Sliding, which is provided with, in slot pushes, and respectively corresponds on the roof and bottom wall of the ontology place position and offers sliding slot one and sliding slot
Two, control panel and alarm lamp, the driving motor, cylinder one, cylinder two, cylinder three, gas are provided on the ontology
Four alarm lamp of cylinder, electric heating tube and headlamp pass through route and are connected with control panel, the lower surface setting of the ontology
There are several universal wheels.
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CN108018197B (en) * | 2018-02-07 | 2018-11-06 | 衢州顺安电子商务有限公司 | A kind of ethyl alcohol bacterial screening equipment |
CN109234161A (en) * | 2018-09-25 | 2019-01-18 | 深圳市宝迪生物工程有限公司 | A kind of rocking equipment and its application method of stem cell medium |
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CN105219676A (en) * | 2015-10-26 | 2016-01-06 | 北京鑫鑫盛达生物工程技术有限公司 | A kind of cultural method of bacillus laterosporus |
CN105879760A (en) * | 2016-04-19 | 2016-08-24 | 成都中牧生物药业有限公司 | Reagent blending method based on circumferential type shaking table |
CN205616906U (en) * | 2016-04-22 | 2016-10-05 | 广东普奥思生物科技有限公司 | Suspended cell culture deep hole dish innovation platform device |
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