CN111423600A - Preparation method of injectable directional conductive hydrogel - Google Patents
Preparation method of injectable directional conductive hydrogel Download PDFInfo
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Abstract
The invention discloses a preparation method of injectable directional conductive hydrogel, which comprises the following steps: step 1: modifying the cellulose nanocrystal with dopamine to obtain polydopamine-modified cellulose nanocrystal; step 2: dispersing the polydopamine-modified cellulose nanocrystals obtained in the step (1) and a conductive polymer monomer in water to form a mixed solution A, adding an initiator, and reacting to obtain a polyconductive polymer-coated cellulose nanocrystal, namely a conductive cellulose nanocrystal; and step 3: dispersing the conductive cellulose nanocrystals and the double-bonded macromolecules obtained in the step (2) in water, and uniformly mixing to form a mixed solution B; adding an additive, adjusting the pH value to be alkaline, and fully reacting to form a hydrogel pre-polymerization liquid; and 4, step 4: extruding the hydrogel pre-polymerization liquid in the step 3 through an extruding device to obtain the directional conductive hydrogel; the hydrogel prepared by the invention has a directional structure similar to a natural tissue, stable conductivity, excellent mechanical property and good biocompatibility.
Description
Technical Field
The invention relates to the technical field of preparation of biological materials, in particular to a preparation method of injectable directional conductive hydrogel.
Background
The conductive hydrogel is a high-quality material integrating dual characteristics of hydrogel flexibility and electron transfer capability of a conductive material, and has been widely applied to the fields of artificial electronic skin, biosensors, tissue engineering materials and the like. In particular, in the field of tissue engineering, electrically conductive hydrogels can enhance the transmission of electrical signals in the body of a living being to facilitate tissue repair. However, the commonly used conductive filling materials, such as metal, graphene or conductive polymer, do not have good water dispersibility, resulting in non-uniform distribution in the hydrogel, and ultimately limiting the exertion of its functions. In addition, the transmission of bioelectrical signals is anisotropic for some specific tissues, such as nerve tissue, cardiac muscle tissue. Therefore, the preparation of the conductive hydrogel capable of inducing the directional transmission of the bioelectricity signals is of great significance.
The cellulose nanocrystal is a one-dimensional nanomaterial with a high aspect ratio, has high mechanical strength and good biocompatibility, and is often used as a template to prepare a one-dimensional functional nanomaterial. In addition, the cellulose nanocrystals also have shear thinning properties and can achieve the purpose of anisotropy after a simple extrusion process. These properties of cellulose nanocrystals inspired us to develop a new method for preparing oriented conducting hydrogels. Namely, the conductive polymer attached to the cellulose nanocrystal is driven to orient in an extrusion mode. However, neither the cellulose nanocrystals nor the conductive polymers have high reactivity, and the weak interaction force causes only a small amount of conductive polymers to be attached to the surface of the cellulose nanocrystals, which greatly limits the application thereof in biomedicine. Therefore, the development of a green conductive cellulose nanocrystal with high conductive polymer loading becomes the key for preparing the oriented conductive hydrogel.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a preparation method of injectable oriented conductive hydrogel with stable conductivity, good biocompatibility and good mechanical property.
The technical scheme adopted by the invention is as follows:
a method of making an injectable, directionally conductive hydrogel, comprising the steps of:
step 1: modifying the cellulose nanocrystal with dopamine to obtain polydopamine-modified cellulose nanocrystal;
step 2, dispersing the polydopamine-modified cellulose nanocrystals obtained in the step 1 and a conductive polymer monomer in water to form a mixed solution A, adding an initiator, and reacting to obtain a polyconductive polymer-coated cellulose nanocrystal, namely a conductive cellulose nanocrystal, wherein the concentration of the polydopamine-modified cellulose nanocrystal in the mixed solution A is 10-100 mg/m L, and the concentration of the conductive polymer monomer is 2-6 mu L/m L;
step 3, dispersing the conductive cellulose nanocrystals and the double-bonded macromolecules obtained in the step 2 in water, uniformly mixing to form a mixed solution B, adding an additive, adjusting the pH to be alkaline, and fully reacting to form a hydrogel pre-polymerization solution, wherein the concentration of the conductive cellulose nanocrystals in the mixed solution B is 10-100 mg/m L, and the concentration of the double-bonded macromolecules is 10-200 mg/m L;
and 4, step 4: and (4) extruding the hydrogel pre-polymerization liquid in the step (3) through an extruding device to obtain the directional conductive hydrogel.
Further, in the step 1, the cellulose nanocrystals are dispersed in dopamine water solution, the pH value is adjusted to be alkaline, and reaction is carried out, wherein in the formed mixed solution, the concentration of the cellulose nanocrystals is 10-100 mg/m L, and the concentration of the dopamine is 5-15 mg/m L.
Further, the pH value is 8-14.
Further, in the step 2, the conductive polymer monomer is one of aniline, pyrrole and 3, 4-ethylenedioxythiophene.
Further, the initiator in the step 2 is one of persulfate, ferric trichloride, ferric tosylate, potassium dichromate, potassium permanganate and peroxide.
Further, the double-bonded polymer in the step 3 is one of double-bonded gelatin, double-bonded chitosan, double-bonded hyaluronic acid, double-bonded sodium alginate, double-bonded collagen, double-bonded K-carrageenan, double-bonded cellulose, double-bonded agarose and polyethylene glycol diacrylate.
Further, the additives in the step 3 are a cross-linking agent and an auxiliary agent, wherein the cross-linking agent is one of ammonium persulfate and potassium persulfate, and the auxiliary agent is tetramethylethylenediamine.
Further, the pH value in the step 3 is 7-14.
Furthermore, the addition amount of the cross-linking agent in the step 3 in the mixed solution B is 5-200 mg/m L, and the addition amount of the auxiliary agent in the mixed solution B is 2-6 μ g/m L.
Further, the extruding device in the step 4 is a syringe or a 3D printing device.
The invention has the beneficial effects that:
(1) the invention adopts polydopamine as a bridging unit of the cellulose nanocrystals and the conductive macromolecules, enhances the adhesion of the conductive macromolecules on the cellulose nanocrystals by utilizing the adhesiveness of the polydopamine, and simultaneously improves the dispersibility of the conductive cellulose nanocrystals in water;
(2) after the conductive nanofiber crystal prepared by the method is mixed with the hydrogel prepolymerization liquid, the orientation of the conductive polymer is completed in a simple extrusion mode, so that the hydrogel with qualitative conductivity and mechanical property is obtained;
(3) the directional conductive hydrogel prepared by the invention has good biocompatibility, has a directional structure and performance similar to natural tissues, can respond to electrical stimulation and transmit directional electrical signals, and is beneficial to regeneration and repair of tissues with directional structures.
Drawings
FIG. 1 is a schematic view of a compressive stress curve in embodiments 1 to 3 of the present invention.
FIG. 2 is a schematic diagram of tensile stress curves of embodiments 1 to 3 of the present invention.
Detailed Description
The invention is further described with reference to the following figures and specific embodiments.
A method of making an injectable, directionally conductive hydrogel, comprising the steps of:
the method comprises the following steps of 1, modifying cellulose nanocrystals with dopamine to obtain polydopamine-modified cellulose nanocrystals, dispersing the cellulose nanocrystals in dopamine water, adjusting the pH value to be alkaline, and reacting, wherein in the formed mixed solution, the concentration of the cellulose nanocrystals is 10-100 mg/m L, the concentration of the dopamine is 5-15 mg/m L, and the pH value is 8-14.
And 2, dispersing the polydopamine-modified cellulose nanocrystal obtained in the step 1 and a conductive polymer monomer in water to form a mixed solution A, adding an initiator, and reacting to obtain the polydopamine-modified cellulose nanocrystal coated by the conductive polymer, namely the conductive cellulose nanocrystal, wherein the concentration of the polydopamine-modified cellulose nanocrystal in the mixed solution A is 10-100 mg/m L, the concentration of the conductive polymer monomer is 2-6 mu L/m L, the conductive polymer monomer is one of aniline, pyrrole and 3, 4-ethylenedioxythiophene, and the initiator is one of persulfate, ferric trichloride, ferric tosylate, potassium dichromate, potassium permanganate and peroxide.
And 3, dispersing the conductive cellulose nanocrystals and the double-bonded macromolecules obtained in the step 2 in water, uniformly mixing to form a mixed solution B, adding an additive, adjusting the pH to be alkaline, and fully reacting to form a hydrogel pre-polymerization solution, wherein the concentration of the conductive cellulose nanocrystals in the mixed solution B is 10-100 mg/m L, the concentration of the double-bonded macromolecules is 10-200 mg/m L, the double-bonded macromolecules are one of double-bonded gelatin, double-bonded chitosan, double-bonded hyaluronic acid, double-bonded sodium alginate, double-bonded collagen, double-bonded K-type carrageenan, double-bonded cellulose, double-bonded agarose and polyethylene glycol diacrylate, the additive is a cross-linking agent and an auxiliary agent, the cross-linking agent is one of ammonium persulfate and potassium persulfate, the auxiliary agent is tetramethylethylenediamine, the pH value is 7-14, the addition amount of the cross-linking agent in the mixed solution B is 5-200 mg/m L, and the addition amount of the auxiliary agent in the mixed solution B is 2-6 [ mu ] g/m L
And 4, step 4: and (4) extruding the hydrogel pre-polymerization liquid in the step (3) through an extruding device to obtain the directional conductive hydrogel. The extrusion device is a syringe or a 3D printing device.
Example 1
A method of making an injectable, directionally conductive hydrogel, comprising the steps of:
step 1, taking 20 mg/m L cellulose nanocrystal solution 10 m L, adding 0.1 g dopamine, stirring and reacting for 30-45 minutes under the condition that the pH value is more than =8 to obtain polydopamine-modified cellulose nanocrystal dispersion liquid, and then centrifuging by using a centrifuge to obtain polydopamine-modified cellulose nanocrystals.
And 2, dispersing 0.2 g of centrifuged polydopamine-modified cellulose nanocrystal in 10 m L deionized water, adding 40 mu L3, 4-ethylenedioxythiophene and 1.35 g of ammonium persulfate, stirring at room temperature for reaction for 12 hours, adding 1.35 g of ammonium persulfate for reaction for 12 hours to obtain the poly 3, 4-ethylenedioxythiophene-modified cellulose nanocrystal, and centrifuging by using a centrifuge to obtain the conductive cellulose nanocrystal.
And 3, dispersing 0.2 g of centrifuged conductive cellulose nanocrystal in 10 m L deionized water to obtain a conductive cellulose nanocrystal solution, taking out 1 m L solution by using a pipette, placing the solution in a 25m L beaker, adding 9 m L deionized water, adding 1.5 g of gelatin, 450 mu L polyethylene glycol diacrylate and 450 mu L polyethylene glycol diglycidyl ether, placing the solution in a 60 ℃ water bath kettle, heating and stirring for 3-5 minutes, adding 20 mu L tetramethylethylenediamine and 0.05 g of ammonium persulfate, and continuously stirring for 5-10 minutes under the condition that the pH is higher than =7 to obtain the hydrogel pre-polymerization liquid.
And 4, step 4: adding the hydrogel prepolymerization solution into an extrusion device (an injector or a 3D printing technology), and obtaining the hydrogel through simple extrusion.
Example 2
A method of making an injectable, directionally conductive hydrogel, comprising the steps of:
step 1, taking 20 mg/m L cellulose nanocrystal solution 10 m L, adding 0.1 g dopamine, stirring and reacting for 30-45 minutes under the condition that the pH value is more than =8 to obtain polydopamine-modified cellulose nanocrystal dispersion liquid, and then centrifuging by using a centrifuge to obtain polydopamine-modified cellulose nanocrystals.
And 2, dispersing 0.2 g of centrifuged polydopamine-modified cellulose nanocrystal in 10 m L deionized water, adding 40 mu L3, 4-ethylenedioxythiophene and 1.35 g of ammonium persulfate, stirring at room temperature for reaction for 12 hours, adding 1.35 g of ammonium persulfate for reaction for 12 hours to obtain the poly 3, 4-ethylenedioxythiophene-modified cellulose nanocrystal, and centrifuging by using a centrifuge to obtain the conductive cellulose nanocrystal.
And 3, dispersing 0.2 g of centrifuged conductive cellulose nanocrystal in 10 m L deionized water to obtain a conductive cellulose nanocrystal solution, taking out a 2 m L solution by using a pipette, placing the solution in a 25m L beaker, adding 8 m L deionized water, adding 1.5 g of gelatin, 450 mu L polyethylene glycol diacrylate and 450 mu L polyethylene glycol diglycidyl ether, placing the solution in a 60 ℃ water bath kettle, heating and stirring for 3-5 minutes, adding 20 mu L tetramethylethylenediamine and 0.05 g of ammonium persulfate, and continuously stirring for 5-10 minutes under the condition that the pH is higher than =7 to obtain the hydrogel pre-polymerization liquid.
And 4, step 4: adding the hydrogel prepolymerization solution into an extrusion device (an injector or a 3D printing technology), and obtaining the hydrogel through simple extrusion.
Example 3
A method of making an injectable, directionally conductive hydrogel, comprising the steps of:
step 1, taking 20 mg/m L cellulose nanocrystal solution 10 m L, adding 0.1 g dopamine, stirring and reacting for 30-45 minutes under the condition that the pH value is more than =8 to obtain polydopamine-modified cellulose nanocrystal dispersion liquid, and then centrifuging by using a centrifuge to obtain polydopamine-modified cellulose nanocrystals.
And 2, dispersing 0.2 g of centrifuged polydopamine-modified cellulose nanocrystal in 10 m L deionized water, adding 40 mu L3, 4-ethylenedioxythiophene and 1.35 g of ammonium persulfate, stirring at room temperature for reaction for 12 hours, adding 1.35 g of ammonium persulfate for reaction for 12 hours to obtain the poly 3, 4-ethylenedioxythiophene-modified cellulose nanocrystal, and centrifuging by using a centrifuge to obtain the conductive cellulose nanocrystal.
And 3, dispersing 0.2 g of centrifuged conductive cellulose nanocrystal in 10 m L deionized water to obtain a conductive cellulose nanocrystal solution, taking out a 4 m L solution by using a pipette, placing the solution in a 25m L beaker, adding 6 m L deionized water, adding 1.5 g of gelatin, 450 mu L polyethylene glycol diacrylate and 450 mu L polyethylene glycol diglycidyl ether, placing the solution in a 60 ℃ water bath kettle, heating and stirring for 3-5 minutes, adding 20 mu L tetramethylethylenediamine and 0.05 g of ammonium persulfate, and continuously stirring for 5-10 minutes under the condition that the pH is higher than =7 to obtain the hydrogel pre-polymerization liquid.
And 4, step 4: adding the hydrogel prepolymerization solution into an extrusion device (an injector or a 3D printing technology), and obtaining the hydrogel through simple extrusion.
Comparative example 1
And (2) adding 1.5 g of gelatin and 450 mu L of polyethylene glycol diglycidyl ether into 10 m of L deionized water, heating and stirring the mixture in a water bath kettle at the temperature of 60 ℃ for 3-5 minutes, adding 20 mu L of tetramethylethylenediamine and 0.05 g of ammonium persulfate, and fully reacting the mixture under the condition that the pH is higher than =7 to obtain the hydrogel GE L + PEGDE.
Comparative example 2
And (2) adding 1.5 g of gelatin, 450 mu L of polyethylene glycol diacrylate and 450 mu L of polyethylene glycol diglycidyl ether into 10 m of L deionized water, heating and stirring the mixture in a water bath kettle at the temperature of 60 ℃ for 3 to 5 minutes, adding 20 mu L of tetramethylethylenediamine and 0.05 g of ammonium persulfate, and fully reacting the mixture under the condition that the pH is higher than =7 to obtain the hydrogel GE L + PEGDE + PEGDA.
FIG. 1 is a compressive stress curve of examples 1 to 3 and comparative examples 1 and 2. FIG. 2 is a tensile stress curve of examples 1 to 3 and comparative examples 1 and 2. Wherein curve A is the hydrogel curve obtained according to the method of example 1, curve B is the hydrogel curve obtained according to the method of example 2, curve C is the hydrogel curve obtained according to the method of example 3, curve D is the hydrogel curve obtained according to the method of comparative example 1, and curve E is the hydrogel curve obtained according to the method of comparative example 2.
As can be seen from FIG. 1, the compressive strength of the hydrogel in comparative example 1 can only reach 0.025MPa, and the compressive strength of the hydrogel in comparative example 2 is 0.18 MPa, the compressive strength of the hydrogel obtained in example 3 reaches 0.45 MPa at most, which is 18 times that of the hydrogel in comparative example 1, which is 2.5 times that of the hydrogel obtained in comparative example 2, the GE L + PEGDE + PEGDA double-network structure can greatly improve the mechanical properties of the hydrogel, and the filling effect of the conductive cellulose nanocrystals in the solution promotes the toughness and strength of the hydrogel, thereby further improving the mechanical properties of the hydrogel.
From FIG. 2, it can be seen that the tensile strength of the hydrogel PEGDE in comparative example 1 is 38 KPa, the tensile strength of the hydrogel GE L + PEGDE + PEGDA obtained in comparative example 2 is 50 KPa. the tensile strength of the hydrogel obtained in example 3 is up to 118 KPa, which is 3 times that of the hydrogel obtained in comparative example 1 and 2 times that of the hydrogel obtained in comparative example 2. the above test results show that the double network structure of the hydrogel and the filling of the conductive cellulose nanocrystals synergistically enhance the mechanical properties of the hydrogel.
Example 4
A method of making an injectable, directionally conductive hydrogel, comprising the steps of:
step 1, taking 20 mg/m L cellulose nanocrystal solution 10 m L, adding 0.1 g dopamine, stirring and reacting for 30-45 minutes under the condition that the pH value is more than =8 to obtain polydopamine-modified cellulose nanocrystal dispersion liquid, and then centrifuging by using a centrifuge to obtain polydopamine-modified cellulose nanocrystals.
And 2, dispersing 0.2 g of centrifuged polydopamine-modified cellulose nanocrystal in 10 m L deionized water, adding 40 mu L3, 4-ethylenedioxythiophene and 1.35 g of ammonium persulfate, stirring at room temperature for reaction for 12 hours, adding 1.35 g of ammonium persulfate for reaction for 12 hours to obtain the poly 3, 4-ethylenedioxythiophene-modified cellulose nanocrystal, and centrifuging by using a centrifuge to obtain the conductive cellulose nanocrystal.
And 3, dispersing 0.2 g of centrifuged conductive cellulose nanocrystals in 10 m L deionized water, adding 1.5 g of gelatin, 600 mu L of polyethylene glycol diacrylate and 600 mu L of polyethylene glycol diglycidyl ether, placing the mixture in a water bath kettle at the temperature of 60 ℃, heating and stirring the mixture for 3 to 5 minutes, adding 20 mu L of tetramethylethylenediamine and 0.05 g of ammonium persulfate, and continuously stirring the mixture for 5 to 10 minutes under the condition that the pH is higher than =7 to obtain the hydrogel pre-polymerization liquid.
And 4, step 4: adding the hydrogel prepolymerization solution into an extrusion device (an injector or a 3D printing technology), and obtaining the hydrogel through simple extrusion.
Example 5
A method of making an injectable, directionally conductive hydrogel, comprising the steps of:
step 1, taking 20 mg/m L cellulose nanocrystal solution 10 m L, adding 0.1 g dopamine, stirring and reacting for 30-45 minutes under the condition that the pH value is more than =8 to obtain polydopamine-modified cellulose nanocrystal dispersion liquid, and then centrifuging by using a centrifuge to obtain polydopamine-modified cellulose nanocrystals.
And 2, dispersing 0.2 g of centrifuged polydopamine-modified cellulose nanocrystal in 10 m L deionized water, adding 40 mu L3, 4-ethylenedioxythiophene and 1.35 g of ammonium persulfate, stirring at room temperature for reaction for 12 hours, adding 1.35 g of ammonium persulfate for reaction for 12 hours to obtain the poly 3, 4-ethylenedioxythiophene-modified cellulose nanocrystal, and centrifuging by using a centrifuge to obtain the conductive cellulose nanocrystal.
And 3, dispersing 0.1 g of centrifuged conductive cellulose nanocrystals in 10 m L deionized water, adding 1.5 g of gelatin, 450 mu L of polyethylene glycol diacrylate and 450 mu L of polyethylene glycol diglycidyl ether, heating and stirring in a 60 ℃ water bath kettle for 3-5 minutes, adding 20 mu L of tetramethylethylenediamine and 0.1 g of ammonium persulfate, and continuously stirring for 5-10 minutes under the condition that the pH is higher than =7 to obtain the hydrogel pre-polymerization liquid.
And 4, step 4: adding the hydrogel prepolymerization solution into an extrusion device (an injector or a 3D printing technology), and obtaining the hydrogel through simple extrusion.
The invention adopts the mussel-like polydopamine with high reactivity as a bridging unit of the cellulose nanocrystals and the conductive polymer, enhances the adhesion of the conductive polymer on the cellulose nanocrystals by utilizing the adhesiveness of the mussel-like polydopamine, and simultaneously improves the dispersibility of the conductive cellulose nanocrystals in water, thereby preparing the one-dimensional conductive nanocrystals based on cellulose. After the prepared conductive cellulose nanocrystals are mixed with the hydrogel prepolymerization solution, the orientation of the conductive macromolecules can be completed in a simple extrusion mode, so that the hydrogel with qualitative conductivity and mechanical property is prepared; the method has simple operation and low cost. The prepared oriented conductive hydrogel has good biocompatibility, has an oriented structure and functions similar to those of natural tissues, can respond to electrical stimulation and transmit oriented electrical signals, and is beneficial to regeneration and repair of tissues with oriented structures.
Claims (10)
1. A method for preparing injectable directionally conductive hydrogel, comprising the steps of:
step 1: modifying the cellulose nanocrystal with dopamine to obtain polydopamine-modified cellulose nanocrystal;
step 2, dispersing the polydopamine-modified cellulose nanocrystals obtained in the step 1 and a conductive polymer monomer in water to form a mixed solution A, adding an initiator, and reacting to obtain a polyconductive polymer-coated cellulose nanocrystal, namely a conductive cellulose nanocrystal, wherein the concentration of the polydopamine-modified cellulose nanocrystal in the mixed solution A is 10-100 mg/m L, and the concentration of the conductive polymer monomer is 2-6 mu L/m L;
step 3, dispersing the conductive cellulose nanocrystals and the double-bonded macromolecules obtained in the step 2 in water, uniformly mixing to form a mixed solution B, adding an additive, adjusting the pH to be alkaline, and fully reacting to form a hydrogel pre-polymerization solution, wherein the concentration of the conductive cellulose nanocrystals in the mixed solution B is 10-100 mg/m L, and the concentration of the double-bonded macromolecules is 10-200 mg/m L;
and 4, step 4: and (4) extruding the hydrogel pre-polymerization liquid in the step (3) through an extruding device to obtain the directional conductive hydrogel.
2. The preparation method of the injectable oriented conductive hydrogel according to claim 1, wherein the cellulose nanocrystals are dispersed in a dopamine aqueous solution in the step 1, and the pH is adjusted to be alkaline to perform a reaction, wherein the cellulose nanocrystals are contained in the mixed solution 10-100 mg/m L, and the concentration of the dopamine is 5-15 mg/m L.
3. The method of claim 2, wherein the pH is 8-14.
4. The method for preparing injectable conductive-oriented hydrogel according to claim 1, wherein the conductive polymer monomer in step 2 is one of aniline, pyrrole and 3, 4-ethylenedioxythiophene.
5. The method for preparing an injectable directionally conductive hydrogel of claim 1 wherein said initiator in step 2 is one of persulfate, ferric chloride, ferric tosylate, potassium dichromate, potassium permanganate, and peroxide.
6. The method for preparing injectable conductive hydrogel of claim 1, wherein the double-bonded polymer in step 3 is one of double-bonded gelatin, double-bonded chitosan, double-bonded hyaluronic acid, double-bonded sodium alginate, double-bonded collagen, double-bonded K-carrageenan, double-bonded cellulose, double-bonded agarose, and polyethylene glycol diacrylate.
7. The method for preparing an injectable electrically-conductive hydrogel of claim 1, wherein the additives in step 3 are a cross-linking agent and an auxiliary agent, wherein the cross-linking agent is one of ammonium persulfate and potassium persulfate, and the auxiliary agent is tetramethylethylenediamine.
8. The method for preparing an injectable electrically conductive hydrogel of claim 1, wherein the pH value in step 3 is 7-14.
9. The method for preparing injectable oriented conductive hydrogel according to claim 7, wherein the amount of the cross-linking agent added in the step 3 in the mixed solution B is 5-200 mg/m L, and the amount of the auxiliary agent added in the mixed solution B is 2-6 μ g/m L.
10. The method for preparing an injectable electrically conductive hydrogel of claim 1, wherein the extruding device in step 4 is a syringe or a 3D printing device.
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