CN111387208A - Botanical pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars and preparation method and application thereof - Google Patents
Botanical pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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Abstract
The invention relates to the technical field of agricultural pest control application, and particularly provides a botanical pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars, and a preparation method and application thereof. The invention aims to provide a plant source pesticide emulsifiable solution preparation for preventing and controlling cabbage caterpillars, which has low concentration and high contact killing effect, does not generate drug resistance, does not have residue on agricultural products after application, and comprises the following components in percentage by weight: 8-15% of arisaema consanguineum alkaloid, 10-30% of emulsifier, 8-23% of cosolvent and the balance of polar organic solvent. In the invention, the arisaema consanguineum alkaloid has high-efficiency insecticidal action and can effectively prevent and control cabbage caterpillar, and field efficacy tests prove that the pesticide preparation of the invention has the effect of preventing and controlling cabbage caterpillar up to 73.8%. The plant source pesticide emulsifiable solution preparation is an effective component for killing insects extracted from plant bodies, is natural, has the characteristics of low toxicity, no residue and the like, and is suitable for preventing and treating cabbage caterpillars.
Description
Technical Field
The invention relates to the technical field of agricultural pest control application, in particular to a botanical pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars and a preparation method and application thereof.
Background
Cabbage caterpillars are larvae of Pieris rapae (also called Pieris rapae), are pests which are most commonly distributed in China, have the most serious harm and are frequently damaged, 9 known host plants are more than 35 plants, and are addicted to cruciferous plants, particularly cabbage, cauliflower, Chinese cabbage, radish and the like which preferentially eat thick leaves. In the absence of cruciferous plants, other host plants may also be consumed. At present, the prevention and treatment of cabbage caterpillars or pieris rapae still mainly adopts a chemical prevention and treatment method.
Although chemical control has the advantage of quick control effect, the residual toxicity of chemical pesticides and a series of environmental and ecological problems caused by the residual toxicity greatly threaten the safety of human and environment. Aiming at the current agricultural sustainable development theory, the development of plant-derived pesticides is a key means for solving the problems, and the preparation of novel, efficient and low-toxicity pesticides by using natural plant active ingredients is valued by people. At present, the alkaloid contained in plants is utilized and is applied more in the aspect of preventing and treating plant diseases and insect pests, and modern pharmacological research shows that the alkaloid has obvious bioactivity and has the effects of resisting tumors, bacteria, oxidation, insect poisoning and the like, and the alkaloid can influence the nervous system, feeding, oviposition and the like of insects.
Perennial herb of Araceae of northeast Araceae is a common traditional Chinese medicine, has the effects of eliminating dampness and phlegm, dispelling pathogenic wind and relieving spasm, and resolving hard mass and detumescence, and has the chemical components mainly comprising alkaloid, flavonoid, polysaccharide, etc. At present, the research on the rhizoma arisaematis has new reports on the aspects of chemical components, pharmacological action, mechanism and the like, but the report that the rhizoma arisaematis alkaloid is prepared into an emulsion preparation for preventing and controlling agricultural pests does not exist at home and abroad.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a botanical pesticide emulsifiable solution preparation for preventing and controlling cabbage caterpillars, which has low concentration and high contact killing effect, does not generate drug resistance and has no residue on agricultural products after application, and a preparation method thereof.
In order to achieve the purpose, the invention provides the following technical scheme: a plant source pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars comprises the following components in percentage by weight: 8-15% of arisaema consanguineum alkaloid, 10-30% of emulsifier, 8-23% of cosolvent and the balance of polar organic solvent.
In the above technical solution, preferably, the polar organic solvent is one or more of absolute ethyl alcohol, acetone, chloroform, ethyl acetate, benzyl alcohol and methanol.
Preferably, the polar organic solvent is one or both of absolute ethanol and methanol.
Preferably, the cosolvent is one or more of low molecular weight alcohols, ethyl acetate and dimethyl sulfoxide.
Preferably, the emulsifier is one or more of tween nonionic surfactant, glycol ester nonionic surfactant, propylene glycol ester nonionic surfactant, polyethylene glycol type nonionic surfactant, fatty acid polyoxyethylene ester, fatty alcohol polyoxyethylene ether and fatty acid polyol ester.
Preferably, the cosolvent is one or two of terpinen-4-ol and dimethyl sulfoxide.
Preferably, the emulsifier is one or two of tween 80 and fatty alcohol-polyoxyethylene ether.
Preferably, the botanical pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars comprises the following components in percentage by weight: 12% of rhizoma arisaematis, 8020% of tween, 18% of terpinene-4-ol and 50% of absolute ethyl alcohol.
The invention also provides a preparation method of the botanical pesticide emulsifiable solution preparation for preventing and treating cabbage caterpillars, which comprises the following steps:
dissolving the arisaema cum bile alkaloid in a polar organic solvent at room temperature to obtain a arisaema cum bile alkaloid solution;
and mixing the arisaema consanguineum alkaloid solution with a cosolvent and an emulsifier to obtain the botanical pesticide emulsifiable solution preparation for preventing and treating cabbage caterpillars.
The invention also provides application of the botanical pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars in the aspect of preventing and treating cabbage caterpillars.
In order to achieve the purpose, the plant source pesticide emulsifiable solution preparation for preventing and controlling cabbage caterpillars is characterized by comprising the following components in percentage by weight: 8-15% of arisaema consanguineum alkaloid, 10-30% of emulsifier, 8-23% of cosolvent and the balance of polar organic solvent. Compared with the prior art, the technical material of the arisaema cum bile in the missible oil is a main active ingredient with drug effect, and the arisaema cum bile alkaloid has high-efficiency insecticidal effect and has various action modes of killing cabbage caterpillars with poison, refusing to eat, avoiding, inhibiting growth and development and the like. The rhizoma arisaematis alkaloids can be combined with ion channel carrier protein to prevent or promote ions from entering biological tissues for transportation, so that normal physiological activities of cabbage caterpillars are interfered, death is caused, and cabbage caterpillars are effectively prevented and treated. The polar organic solvent in the preparation is used for dissolving and diluting the original medicine, so that the original medicine is dispersed into stable homogeneous liquid, and the fluidity of the missible oil preparation is improved; the cosolvent is used for promoting the dissolution of the original medicine in the organic solvent; the emulsifier reduces the surface energy of oil phase formed by raw pesticide and organic solvent, so that the raw pesticide in the emulsifiable concentrate preparation can be uniformly dispersed in water in the later use process to form stable emulsion. Therefore, the complete preparation formula provided by the invention has the excellent effects of low toxicity, high efficiency, stability and good environmental compatibility. The field pesticide effect test of the emulsifiable solution preparation provided by the invention proves that the control effect of the pesticide preparation provided by the invention on cabbage caterpillar can reach 73.8%. Moreover, the invention has the following advantages:
1. the plant source pesticide emulsifiable concentrate preparation prepared by the invention has the advantages of rich raw material sources, low price, easy obtainment, simple preparation process and low requirement on equipment.
2. The plant source pesticide emulsifiable solution preparation is an effective component for killing insects extracted from plant bodies, is natural, environment-friendly, low-toxic, residue-free and the like, and is suitable for preventing and treating cabbage caterpillars.
Detailed Description
The invention provides a botanical pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars, which comprises the following components in percentage by weight: 8-15% of arisaema consanguineum alkaloid, 20-30% of emulsifier, 18-23% of cosolvent and the balance of polar organic solvent.
The botanical pesticide emulsifiable solution preparation provided by the invention comprises 8-15 wt% of arisaema consanguineum alkaloid, preferably 10-15 wt%, and more preferably 12-15 wt%. The source of the arisaema cum bile alkaloid is not particularly limited, and the arisaema cum bile alkaloid can be prepared by a preparation process known by persons skilled in the art. In the embodiment of the present invention, the extraction method of the arisaema cum bile alkaloids preferably includes the following steps:
cleaning, drying and crushing tuber of the rhizoma arisaematis, and preparing to obtain rhizoma arisaematis medicinal powder;
mixing the rhizoma arisaematis powder with an ethanol solution, and performing reflux extraction to obtain an extracting solution;
and eluting and purifying the extracting solution by adopting HPD-300 type resin, wherein the eluent is water and ethanol, and the arisaema cum bile alkaloid in northeast is obtained.
After the powder of the northeast rhizoma arisaematis is obtained, the powder of the northeast rhizoma arisaematis is extracted by reflux (refer to the study on the extraction and purification process of alkaloids in traditional Chinese medicine of the northeast rhizoma arisaematis. on the 10 th stage 2343-2345 page of the 26 th volume of the Shizhen national medicine 2015). In the invention, during the reflux extraction, the dosage of the ethanol solution is preferably 20 times of the mass of the rhizoma arisaematis powder; the volume fraction of the ethanol solution is preferably 60%, the reflux extraction frequency is preferably 2 times, and the time length of each extraction is preferably 2 hours.
After obtaining the extracting solution, the invention preferably performs resin purification on the extracting solution (see paper, "the extraction and purification process research of alkaloids in rhizoma arisaematis in northeast China" of precious national medicine 2015, 26, 10, pages 2343-2345.) in the invention, the extracting solution is preferably evaporated by a rotary evaporator to obtain a paste extract, the paste extract is prepared into a sample loading solution, the concentration of the sample loading solution is preferably 0.1g/m L, the mass ratio of the sample loading amount of the sample loading solution to the dry resin is preferably 1:1, the using amount of eluent water is preferably 5BV, and the using amount of 70% ethanol (volume fraction) of the eluent is preferably 5 BV.
In the invention, the eluent water is used for eluting impurity components, and the eluent 70% ethanol is used for eluting and purifying arisaema alkaloid.
The botanical pesticide emulsifiable solution preparation provided by the invention comprises 8-15 wt% of arisaema consanguineum alkaloid, preferably 10-15 wt%, more preferably 12-15 wt%, and in the invention, the arisaema consanguineum alkaloid has various action modes of poisoning, food refusing, avoiding, growth and development inhibiting and the like on cabbage caterpillars. The arisaema consanguineum alkaloid can prevent or promote ions to enter biological tissues for transportation by combining with ion channel carrier protein, thereby interfering the normal physiological activity of the cabbage caterpillar and causing death.
The plant source pesticide emulsifiable solution preparation provided by the invention comprises 10-30% of emulsifier by weight, preferably 10-25%; in the invention, the emulsifier is one or more of tween nonionic surfactant, glycol ester nonionic surfactant, propylene glycol ester nonionic surfactant, polyethylene glycol type nonionic surfactant, fatty acid polyoxyethylene ester, fatty alcohol polyoxyethylene ether and fatty acid polyol ester, and more preferably one or two of tween 80 and fatty alcohol polyoxyethylene ether.
In the invention, the emulsifier can reduce the surface energy of an oil phase formed by the raw pesticide and the organic solvent, so that the raw pesticide in the missible oil preparation can be uniformly dispersed in water in the later use process to form stable emulsion.
The plant source pesticide emulsifiable solution preparation provided by the invention comprises 8-23 wt% of cosolvent, preferably 8-18 wt%; in the present invention, the cosolvent is preferably one or more of low-component alcohols, ethyl acetate and dimethyl sulfoxide, and is more preferably one or two of terpinen-4-ol and dimethyl sulfoxide.
The plant source pesticide emulsifiable solution preparation provided by the invention comprises the balance of polar organic solvent; in the present invention, the polar organic solvent is preferably one or more of absolute ethanol, acetone, chloroform, ethyl acetate, benzyl alcohol, and methanol, and is more preferably absolute ethanol.
In the invention, the polar organic solvent in the preparation dissolves and dilutes the original medicine, so that the original medicine is dispersed into stable homogeneous liquid, and the fluidity of the missible oil preparation is improved; the cosolvent can promote the dissolution of the original drug in the solvent.
In the invention, the botanical pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars further preferably comprises the following components in percentage by weight: 12% of rhizoma arisaematis, 8020% of tween, 18% of terpinene-4-ol and 50% of absolute ethyl alcohol.
The invention also provides a preparation method of the plant source pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars, which comprises the following steps:
dissolving the arisaema cum bile alkaloid in a polar organic solvent at room temperature to obtain a arisaema cum bile alkaloid solution;
and mixing the arisaema consanguineum alkaloid solution with a cosolvent and an emulsifier to obtain the botanical pesticide emulsifiable solution preparation for preventing and treating cabbage caterpillars.
In the invention, no special requirement is required for the process of dissolving the arisaema cum bile alkaloid by using the polar organic solvent, and a dissolving method which is well known by the technical personnel in the field can be adopted.
In the invention, the process of mixing the arisaema cum bile alkaloid solution with the cosolvent and the emulsifier has no special requirement, and a mixing method which is well known by the technical personnel in the field can be adopted.
The invention also provides application of the botanical pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars in the aspect of preventing and treating cabbage caterpillars.
Preferably, the botanical pesticide emulsifiable solution preparation is applied to the prevention and treatment of cabbage caterpillars by spraying at normal temperature, and is diluted by 10-20 times with clear water before use, and the diluted dosage is 0.3-1.2 g/667m2More preferably 1.2g/667m2。
Compared with the prior art, the arisaema consanguineum alkaloid has high-efficiency insecticidal action and can effectively prevent and control cabbage caterpillars, and field pesticide effect tests prove that the pesticide preparation has the effect of preventing and controlling cabbage caterpillars as high as 73.8%. Moreover, the invention has the following advantages:
1. the plant source pesticide emulsifiable concentrate preparation prepared by the invention has the advantages of rich raw material sources, low price, easy obtainment, simple preparation process and low requirement on equipment.
2. The plant source pesticide emulsifiable solution preparation is an effective component for killing insects extracted from plant bodies, is natural, environment-friendly, low-toxic, residue-free and the like, and is suitable for preventing and treating cabbage caterpillars.
In order to better understand the present invention, the following examples are further provided to illustrate the present invention, but the present invention is not limited to the following examples.
Example 1
Calculated by weight percentage, 12 percent of arisaema consanguineum alkaloid, 8020 percent of tween, 18 percent of terpinene-4-ol and 50 percent of absolute ethyl alcohol;
dissolving the arisaema cum bile alkaloid in absolute ethyl alcohol at room temperature to obtain the arisaema cum bile alkaloid solution, and mixing the arisaema cum bile alkaloid solution with terpinene-4-alcohol and Tween 80.
Wherein, the extraction and purification process of the alkaloid of the Arisaema cum bile is as follows (see the study of the extraction and purification process of the alkaloid component of the Arisaema cum bile of traditional Chinese medicine' Shizhen national medicine 2015, volume 26, No. 10, pages 2343-2345),
cleaning, drying and crushing a raw material medicinal material of the northeast rhizoma arisaematis (tuber of the northeast rhizoma arisaematis), and preparing rhizoma arisaematis medicinal material powder; then adding 20 times of 60% ethanol solution in volume fraction into the medicinal powder, and reflux-extracting for 2 times, each time for 2h to obtain extractive solution; then sample is carried out by HPD-300 resin and 0.1g/ml concentration extract 1:1 (medicinal material: dry resin) proportion, 5BV water is used for eluting and removing impurities, and 5BV 70% (volume fraction) ethanol is used for eluting and purifying.
Example 2
Calculated by weight percentage, 15 percent of arisaema consanguineum alkaloid, 0 percent of tween 8010 percent of terpinene-4-ol and 77 percent of absolute ethyl alcohol;
dissolving the arisaema cum bile alkaloid in absolute ethyl alcohol at room temperature to obtain the arisaema cum bile alkaloid solution, and mixing the arisaema cum bile alkaloid solution with terpinene-4-alcohol and Tween 80.
Wherein the extraction and purification process of the arisaema cum bile alkaloid comprises the following steps,
cleaning, drying and crushing a raw material medicinal material of the northeast rhizoma arisaematis (tuber of the northeast rhizoma arisaematis), and preparing rhizoma arisaematis medicinal material powder; then adding 20 times of 60% ethanol solution in volume fraction into the medicinal powder, and reflux-extracting for 2 times, each time for 2h to obtain extractive solution; then sample is carried out by HPD-300 resin and 0.1g/ml concentration extract 1:1 (medicinal material: dry resin) proportion, 5BV water is used for eluting and removing impurities, and 5BV 70% (volume fraction) ethanol is used for eluting and purifying.
Example 3
Calculated by weight percentage, 15 percent of arisaema consanguineum alkaloid, 10 percent of fatty alcohol-polyoxyethylene ether (JFC), 8 percent of dimethyl sulfoxide and 77 percent of absolute ethyl alcohol;
dissolving the arisaema cum bile alkaloid with absolute ethyl alcohol at room temperature to obtain the arisaema cum bile alkaloid solution, and mixing the arisaema cum bile alkaloid solution with fatty alcohol-polyoxyethylene ether (JFC) and dimethyl sulfoxide.
Wherein the extraction and purification process of the arisaema cum bile alkaloid comprises the following steps,
cleaning, drying and crushing a raw material medicinal material of the northeast rhizoma arisaematis (tuber of the northeast rhizoma arisaematis), and preparing rhizoma arisaematis medicinal material powder; then adding 20 times of 60% ethanol solution in volume fraction into the medicinal powder, and reflux-extracting for 2 times, each time for 2h to obtain extractive solution; then sample is carried out by HPD-300 resin and 0.1g/ml concentration extract 1:1 (medicinal material: dry resin) proportion, 5BV water is used for eluting and removing impurities, and 5BV 70% (volume fraction) ethanol is used for eluting and purifying.
Example 4
Indoor toxicology test tests were performed on the botanical pesticide emulsifiable concentrate preparation prepared in example 1 on cabbage caterpillars.
In order to determine the insecticidal activity of the prepared plant source pesticide emulsifiable solution preparation on cabbage caterpillars, a contact activity test is carried out indoors. The prepared missible oil is diluted by 10, 12, 15, 18 and 20 times respectively by using clear water as a reference, and the contact poisoning effect of the prepared missible oil preparation on cabbage caterpillars is determined. Table 1 shows the toxicity test results of the plant-derived pesticide emulsifiable concentrate preparation prepared in example 1 against cabbage caterpillar.
TABLE 1 determination of toxicity of plant-derived pesticide emulsifiable concentrate preparation on cabbage caterpillar
As can be seen from the data in Table 1, the plant-derived pesticide emulsifiable concentrate preparation has remarkable poisoning effect on cabbage caterpillars, and L C of 24h and 48h5043.21 mg/L and 28.95 mg/L respectively, and the poisoning effect is stronger than the insecticidal effect in 24 hours in 48 hours.
Example 5
The field efficacy test of the plant source pesticide emulsifiable solution preparation prepared in example 1 on cabbage caterpillar is carried out. The field pesticide effect test is evaluated and researched at the teaching test base of the institute of Life sciences of the general chemical institute of teachers and professors, the field pesticide effect test is processed in a spraying mode, and the concentrations of plant source pesticide emulsifiable oil preparations are set to be 1.2g/667m respectively2，0.6g/667m2，0.3g/667m2Setting the concentration of 1.8% avermectin emulsion as reference agent as 0.12g/667m2(ii) a For a total of 4 treatments. Each process set 3 replicates. Random block arrangement, cell area 10m2.And selecting continuous 10 Chinese cabbages in each cell, and respectively calculating the number of live insects and the control efficiency which are different from 1d to 3d after application, wherein the experimental results are shown in a table 2.
Table 2 determination of field potency toxicity of the plant-derived pesticide emulsifiable concentrate preparation prepared in example 1 against cabbage caterpillar
As can be seen from the data in Table 2, the preparation was made after applying 1dThe prepared emulsifiable concentrate preparation is 1.2g/667m2The control effect of the dosage on the cabbage caterpillar is 0.12g/667m compared with that of a control medicament 1.8 percent avermectin missible oil2The control effect is equivalent, and the prepared missible oil preparation is 1.2g/667m after the pesticide is applied for 3 days2The control effect of the dosage on the cabbage caterpillar is higher than that of a control medicament 1.8 percent of abamectin emulsifiable solution.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (10)
1. A plant source pesticide emulsifiable concentrate preparation for preventing and treating cabbage caterpillars is characterized by comprising the following components in percentage by weight: 8-15% of arisaema consanguineum alkaloid, 10-30% of emulsifier, 8-23% of cosolvent and the balance of polar organic solvent.
2. The plant-derived pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in claim 1, wherein the polar organic solvent is one or more of absolute ethanol, acetone, chloroform, ethyl acetate, benzyl alcohol and methanol.
3. The plant-derived pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in claim 2, wherein the polar organic solvent is one or both of absolute ethanol and methanol.
4. The plant-derived pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in claim 1, wherein the cosolvent is one or more of low molecular weight alcohols, ethyl acetate and dimethyl sulfoxide.
5. The plant-derived pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in claim 1, wherein the emulsifier is one or more of tween nonionic surfactant, glycol ester nonionic surfactant, propylene glycol ester nonionic surfactant, polyethylene glycol type nonionic surfactant, fatty acid polyoxyethylene ester, fatty alcohol polyoxyethylene ether and fatty acid polyol ester.
6. The plant-derived pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in claim 4, wherein the cosolvent is one or two of terpinen-4-ol and dimethyl sulfoxide.
7. The plant-derived pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in claim 5, wherein the emulsifier is one or both of tween 80 and fatty alcohol-polyoxyethylene ether.
8. The plant source pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in any one of claims 1 to 7, which comprises the following components in percentage by weight: 12% of rhizoma arisaematis, 8020% of tween, 18% of terpinene-4-ol and 50% of absolute ethyl alcohol.
9. The method for preparing a plant-derived pesticide emulsifiable concentrate preparation for controlling cabbage caterpillars as claimed in any one of claims 1 to 8, comprising the steps of:
dissolving the arisaema cum bile alkaloid in a polar organic solvent at room temperature to obtain a arisaema cum bile alkaloid solution;
and mixing the arisaema consanguineum alkaloid solution with a cosolvent and an emulsifier to obtain the botanical pesticide emulsifiable solution preparation for preventing and treating cabbage caterpillars.
10. The use of the emulsifiable concentrate preparation of a botanical pesticide for controlling cabbage caterpillars as claimed in any one of claims 1 to 8 or the emulsifiable concentrate preparation of a botanical pesticide for controlling cabbage caterpillars obtained by the preparation method as claimed in claim 9 for controlling cabbage caterpillars.
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| CN104322572A (en) * | 2014-10-24 | 2015-02-04 | 河南省科高植物天然产物开发工程技术有限公司 | Botanical insecticide |
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| CN105454321A (en) * | 2015-12-16 | 2016-04-06 | 胡彩军 | Biological insecticide for preventing and controlling cabbage caterpillars and preparation method thereof |
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2020
- 2020-04-09 CN CN202010272801.9A patent/CN111387208A/en active Pending
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| KR20150095329A (en) * | 2014-02-13 | 2015-08-21 | 송상민 | Method of producing natural insecticides |
| CN104322572A (en) * | 2014-10-24 | 2015-02-04 | 河南省科高植物天然产物开发工程技术有限公司 | Botanical insecticide |
| CN105454321A (en) * | 2015-12-16 | 2016-04-06 | 胡彩军 | Biological insecticide for preventing and controlling cabbage caterpillars and preparation method thereof |
| CN107836461A (en) * | 2017-11-24 | 2018-03-27 | 山东农业大学 | Eco-friendly pesticide missible oil |
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