CN111378565B

CN111378565B - Microbial cultivation device

Info

Publication number: CN111378565B
Application number: CN202010196795.3A
Authority: CN
Inventors: 马素珍; 孙玉雷; 姜永孝
Original assignee: Henan University of Traditional Chinese Medicine HUTCM
Current assignee: Henan University of Traditional Chinese Medicine HUTCM
Priority date: 2020-03-19
Filing date: 2020-03-19
Publication date: 2022-12-06
Anticipated expiration: 2040-03-19
Also published as: CN111378565A

Abstract

The invention discloses a microbial culture device, and belongs to the technical field of biology. The culture device comprises. The culture device comprises a culture container, wherein an air bag ring is arranged at the bottom of the culture container, the air bag ring is connected with an inflation tube, the inflation tube penetrates out of the culture container and is connected with an inflation pump, an exhaust tube communicated with the air bag ring is further connected to the inflation tube, an exhaust valve is arranged on the exhaust tube, the culture container comprises an inner container and a shell, an outward extending edge is arranged at the top of the inner container and is fixedly connected with the shell, the bottom of the inner container is located above the bottom of the shell, a concave-convex structure is arranged on the side wall of the inner container, a water filling port is arranged on the extending edge, a heating device is arranged in a space formed by the inner container and the shell, and a water outlet is arranged at the bottom of the shell.

Description

Microbial cultivation device

Technical Field

The invention relates to the technical field of biology, in particular to a microorganism culture device.

Background

The microbial culture technology can meet the requirements of microbial nutrition and certain environmental conditions, and can be used as an operation technology for rapidly growing and proliferating, expressing the physiological action of the microorganism or producing a certain metabolite. During the process of culturing the microorganisms, the microorganisms secrete organic acid or alkaline metabolites, so that the pH of the culture solution fluctuates, and therefore, the pH of the culture solution needs to be detected and timely adjusted.

Adopt the agitator to stir the pH regulator of adding usually among the prior art, so that the pH regulator of adding can be fast even dispersion in the culture solution, form regular circular motion easily in stirring process, dispersion speed is relatively slow, also in the prior art for realizing rapid mixing, agitating unit sets up a plurality of stirring vane from bottom to top, because stirring vane's increase, the resistance that receives during the stirring is big more, the power consumption of motor is big more, and this stirring mode realizes that the culture solution is even inefficiency from top to bottom, consequently need further improvement.

Disclosure of Invention

The invention aims to overcome the problems in the prior art and provide a microorganism culture device.

The microbial cultivation device provided by the invention comprises a cultivation container, wherein an air bag ring is arranged at the bottom of the cultivation container, the air bag ring is connected with an inflation tube, the inflation tube penetrates out of the cultivation container and is connected with an inflation pump, an exhaust pipe communicated with the air bag ring is further connected to the inflation tube, and an exhaust valve is arranged on the exhaust pipe;

the culture container comprises an inner container and a shell, wherein the top of the inner container is provided with an outward extending edge, the extending edge is fixedly connected with the shell, the bottom of the inner container is positioned above the bottom of the shell, the side wall of the inner container is provided with a concave-convex structure, the extending edge is provided with a water injection port, a heating device is arranged in a space between the inner container and the shell, and the bottom of the shell is provided with a water outlet.

Preferably, the balloon ring is adhered to the bottom of the culture container by an adhesive.

Preferably, the concave-convex structure is a corrugated structure uniformly distributed along the peripheral wall of the inner container.

Preferably, the culture container is provided with a nutrient solution injection pipe and a neutralizer adding pipe, the bottom of the culture container is provided with a liquid discharge pipe, and the nutrient solution injection pipe, the neutralizer adding pipe and the liquid discharge pipe are provided with valves.

Preferably, the neutralizer adding pipe is connected with the nutrient solution injection pipe.

Preferably, the exhaust valve is a solenoid valve, and the inflator and the solenoid valve for exhausting are both electrically connected with the controller.

Preferably, a temperature sensor is arranged in the inner container, and the temperature sensor and the heating device are electrically connected with the controller.

Preferably, a pH value sensor is arranged in the inner container, the pH value sensor is electrically connected with the controller, and the controller is also electrically connected with an alarm device for prompting pH value abnormity.

Preferably, the shell is further provided with a control panel, the control panel is provided with a display screen for displaying detection values, and the control panel and the display screen are both electrically connected with the controller.

Compared with the prior art, the invention has the beneficial effects that: according to the culture device, the air bag rings are arranged at the bottom of the culture container, and the air bag rings are used for periodic inflation and exhaust to form turbulent flow in each direction, so that the pH value of nutrient solution, oxygen and nutrient substances in the culture container are balanced everywhere, and compared with a traditional stirring device, the periodic inflation and exhaust of the air bag rings has the advantages of diversification of turbulent flow directions, improvement of uniform mixing effect and no need of large energy consumption.

By adopting the culture device, when the detection shows that new nutrient solution needs to be supplemented or the neutralizer needs to be added in the culture process, the nutrient solution or the neutralizer is added from the top of the culture container, the liquid in the culture container is subjected to the impulsive force from the newly added liquid at the upper part, and the middle part and the lower part are subjected to the turbulent flow formed by the periodic inflation and exhaust of the air bag ring, so that the newly added nutrient solution or the neutralizer is uniformly and rapidly distributed.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the bladder of the present invention in its inner bladder configuration;

fig. 3 is a top view of the liner of the present invention.

Description of the reference numerals:

1. the culture container comprises a culture container body, 11 parts of an inner container, 12 parts of a shell, 2 parts of an air bag ring, 3 parts of an inflation pipe, 4 parts of a nutrient solution injection pipe, 5 parts of a neutralizing agent addition pipe, 6 parts of a liquid discharge pipe, 7 parts of an extending edge, 8 parts of a concave-convex structure, 9 parts of a water injection port, 10 parts of a heating device, 13 parts of a water discharge port, 14 parts of a control panel and 15 parts of a display screen.

Detailed Description

Detailed description of the preferred embodimentsthe following detailed description of the present invention will be made with reference to the accompanying drawings 1-3, although it should be understood that the scope of the present invention is not limited to the specific embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

The invention provides a microorganism culture device, which comprises a culture container 1, wherein the bottom of the culture container 1 is provided with an air bag ring 2, the air bag ring 2 is connected with an inflation tube 3, the inflation tube 3 penetrates out of the culture container 1 to be connected with an inflation pump, the inflation tube 3 is also connected with an exhaust pipe communicated with the air bag ring 2, and the exhaust pipe is provided with an exhaust valve;

culture container 1 includes inner bag 11 and shell 12, and the top of inner bag 11 is equipped with outside extension limit 7, extend limit 7 and shell 12 fixed connection, and the bottom of inner bag 11 is located the top of shell 12 bottom, be equipped with concave-convex structure 8 on the lateral wall of inner bag 11, be equipped with water filling port 9 on the extension limit 7, be equipped with heating device 10 in the space between inner bag 11 and the shell 12, shell 12 bottom is equipped with outlet 13.

The air bag ring 2 is disposed at the bottom of the culture container in this embodiment, and is aimed at disturbing the nutrient solution in the culture container 1 so that the nutrient is uniformly distributed, and since the nutrient is most consumed at the position where the microorganism is gathered and the pH value of the nutrient solution is likely to be affected by the product of the microorganism, it is necessary to uniformly distribute the nutrient by the bypass flow and maintain a proper culture environment. The embodiment specifically comprises the steps that an air bag is inflated through an inflator pump, then air is exhausted through an exhaust valve, so that the air bag deforms, and the air bag is repeatedly inflated and exhausted to achieve the purpose of streaming; besides, be equipped with concave-convex structure 8 on the lateral wall of inner bag 11 of this embodiment, concave-convex structure 8's one of effect lies in forming the unevenness structure including bulge and concave recess on the inner bag 11 lateral wall, and liquid can flow to inner bag 11 lateral wall under gasbag ring 2's promotion after gasbag ring 2 aerifys, and the liquid that flows produces different velocity of flow and different flow direction after producing the collision with the concave recess and the bulge of lateral wall respectively, and the fluidic diversification must be with the purpose of effective quick realization even nutrient solution.

The culture container 1 of this embodiment is designed in a double layer, and the gap between the inner container 11 and the outer casing 12 can contain hot water, so that the culture container 1 can be heated in a water bath as required, and the second function of the concave-convex structure 8 is to increase the surface area of the side wall of the inner container 11 and increase the heat transfer effect.

Wherein, the air sac ring 2 is adhered to the bottom of the culture container 1 through an adhesive.

The concave-convex structure 8 is a corrugated structure uniformly distributed along the peripheral wall of the inner container 11.

Wherein, a nutrient solution injection pipe 4 and a neutralizer adding pipe 5 are arranged on the culture container 1, a liquid discharge pipe 6 is arranged at the bottom of the culture container 1, and valves are arranged on the nutrient solution injection pipe 4, the neutralizer adding pipe 5 and the liquid discharge pipe 6. The nutrient solution injection tube 4 and the neutralizing agent addition tube 5 are provided above the culture vessel 1 to disturb the upper layer liquid.

Wherein, the neutralizer adding pipe 5 is connected with the nutrient solution injection pipe 4. The two are in contact in the adding process, so that the mixing is convenient.

The air outlet valve is an electromagnetic valve, and the inflator pump and the electromagnetic valve for air outlet are electrically connected with the controller.

Wherein, be equipped with temperature sensor in the inner bag 11, temperature sensor and heating device 10 all are connected with the controller electricity. The purpose is to control the temperature of the internal liquid and provide a suitable growing environment.

Wherein, be equipped with pH value sensor in the inner bag 11, pH value sensor is connected with the controller electricity, the controller still electricity is connected with the alarm device who is used for indicateing pH value unusual. The pH value is detected by the pH value sensor, and the alarm device is used for reminding timely adjustment.

Wherein, still be equipped with control panel 14 on the shell 12, be equipped with the display screen 15 that is used for showing the detected value on the control panel 14, control panel 14 and display screen 15 all are connected with the controller electricity.

In summary, the culture device of the invention forms turbulent flow in each direction by arranging the air bag ring at the bottom of the culture container and utilizing the periodic inflation and exhaust of the air bag ring, so that the pH value of the nutrient solution, oxygen and nutrient substances in the culture container are balanced everywhere, and compared with the traditional stirring device, the periodic inflation and exhaust of the air bag ring has the advantages of diversification of turbulent flow directions, improved uniform mixing effect and no need of larger energy consumption.

By adopting the culture device, when the detection shows that new nutrient solution needs to be supplemented or the neutralizer needs to be added in the culture process, the nutrient solution or the neutralizer is added from the top of the culture container, the liquid in the culture container is impacted by the newly added liquid from the upper part, and the middle and lower parts of the liquid are subjected to turbulent flow formed by periodic inflation and exhaust of the air bag ring, so that the newly added nutrient solution or the neutralizer is uniformly and quickly distributed.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The microbial cultivation device is characterized by comprising a cultivation container (1), wherein an air bag ring (2) is arranged at the bottom of the cultivation container (1), the air bag ring (2) is connected with an inflation tube (3), the inflation tube (3) penetrates out of the cultivation container (1) to be connected with an inflation pump, the inflation tube (3) is further connected with an exhaust pipe communicated with the air bag ring (2), and the exhaust pipe is provided with an exhaust valve;

the culture container (1) comprises a liner (11) and a shell (12), wherein an outward extending edge (7) is arranged at the top of the liner (11), the extending edge (7) is fixedly connected with the shell (12), the bottom of the liner (11) is positioned above the bottom of the shell (12), a concave-convex structure (8) is arranged on the side wall of the liner (11), and the concave-convex structure (8) is a corrugated structure uniformly distributed along the peripheral wall of the liner (11); a water filling port (9) is arranged on the extending edge (7), a heating device (10) is arranged in a space between the inner container (11) and the shell (12), and a water outlet (13) is arranged at the bottom of the shell (12);

the culture container (1) is provided with a nutrient solution injection pipe (4) and a neutralizer adding pipe (5), the bottom of the culture container (1) is provided with a liquid discharge pipe (6), and the nutrient solution injection pipe (4), the neutralizer adding pipe (5) and the liquid discharge pipe (6) are provided with valves.

2. The microorganism culture apparatus according to claim 1, wherein the balloon ring (2) is adhered to the bottom of the culture vessel (1) by an adhesive.

3. The microorganism culture apparatus according to claim 1, wherein the neutralizer adding tube (5) is connected to the nutrient solution injecting tube (4).

4. The microorganism culture apparatus according to claim 1, wherein the exhaust valve is a solenoid valve, and the inflator and the solenoid valve for exhausting are electrically connected to the controller.

5. The microorganism culture apparatus according to claim 4, wherein a temperature sensor is provided in the inner container (11), and the temperature sensor and the heating device (10) are electrically connected to the controller.

6. The microorganism culture device according to claim 5, characterized in that a pH sensor is arranged in the inner container (11), the pH sensor is electrically connected with a controller, and the controller is also electrically connected with an alarm device for prompting pH abnormality.

7. The microorganism culture apparatus according to claim 6, wherein the housing (12) is further provided with a control panel (14), the control panel (14) is provided with a display screen (15) for displaying the detection value, and the control panel (14) and the display screen (15) are both electrically connected with the controller.