CN111377773A - Seaweed compound microbial organic fertilizer containing endophytes and used for peanuts - Google Patents
Seaweed compound microbial organic fertilizer containing endophytes and used for peanuts Download PDFInfo
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- CN111377773A CN111377773A CN202010297950.0A CN202010297950A CN111377773A CN 111377773 A CN111377773 A CN 111377773A CN 202010297950 A CN202010297950 A CN 202010297950A CN 111377773 A CN111377773 A CN 111377773A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention belongs to the field of agricultural fertilizers, and particularly relates to a seaweed compound microbial organic fertilizer containing endophytes and used for peanuts. The organic fertilizer provided by the invention comprises the following raw materials in parts by weight: 400-600 parts of plant source organic matter, 300-400 parts of animal source organic matter, 100-200 parts of activated humic acid, 40-80 parts of medium trace elements, 10-20 parts of a nutrition regulator, 1-20 parts of a compound microbial agent and 5-10 parts of chitin. The compound microbial fertilizer containing the endophytic bacterial strains separated from the roots of the peanuts is adopted, and other endophytic bacteria are not adopted, so that the compound microbial fertilizer is more favorable for being colonized in the peanut plants, can survive and grow in the peanut plants, particularly in the root systems, and plays a role in promoting the growth; the invention adopts chitin, can activate the autoimmune system of plants to realize the disease-resistant effect, is non-toxic and harmless, and reduces the disease and insect resistance of peanut plants.
Description
Technical Field
The invention belongs to the field of agricultural fertilizers, and particularly relates to a seaweed compound microbial organic fertilizer containing endophytes and used for peanuts.
Background
Peanuts are one of important edible oil and protein sources, and the main problems existing in peanut planting at present are as follows: 1. unreasonable fertilizer use, complex soil types, various peanut varieties and lack of special fertilizer products which are targeted and can meet the requirements of high-yield and high-efficiency production of peanuts in different areas; 2. excessive nitrogen application in the early stage inhibits the exertion of the nitrogen fixation potential of the peanuts, and the continuous nitrogen supply capability of the soil in the middle and later stages to the peanuts is insufficient, so that premature senility is easy to occur, and the yield and the planting benefit of the peanuts are influenced.
CN108129189A discloses a urea sulfate microbial fertilizer special for peanuts, which is characterized in that: the urea sulfate microbial fertilizer special for peanuts comprises the following raw materials, by weight, 100-140 parts of urea sulfate, 120-180 parts of urea, 110-140 parts of potassium sulfate, 100-150 parts of potassium nitrate, 280-335 parts of monoammonium phosphate, 70-90 parts of a compound microbial agent, 60-70 parts of a urea sulfate slow-release function enhancer, 18-22 parts of a urea sulfate fertilizer microbial growth promoter, 0-10 parts of a filler and 10-12 parts of an anti-caking agent.
In the above patent application, the complex microbial agents are used, but it is not clear what complex microbial agents are specifically used, and different kinds of complex microbial agents have different effects or even have the same effect but have different significance; for example, in Zhejiang agriculture and forestry university leaf 26107Shuo, the Master thesis "prevention and control effect of different microbial agents on pepper phytophthora blight and influence on soil traits" discloses: all results show that the compounded results are not good as the results of single strains, and probably are caused by the antagonistic effect among the strains. Zhao Guang quan et al in the text of "field control effect of 4 microbial agents on mango main diseases" proved by experiments: the Wofengkang-compound microbial agent has the best prevention effect on mango anthracnose, the prevention effect is 60.00 percent or more, and the Wofengkang-paecilomyces lilacinus microbial fertilizer has the prevention effect of over 54 percent. The Voofengkang-compound microbial inoculant has better prevention effect on mango gray leaf spot, and reaches 56.13%; secondly, the microbial agent for Zhongnong green health has the prevention effect of 49.91 percent. The control effect of the 4 microbial agents on mango powdery mildew is poor.
From the above documents, it can be seen that the composite microbial agents are also composite microbial agents, but due to different species compositions and different species dosages in each composite microbial agent, there is a possibility that synergistic effects may occur among the species, and there is a possibility that antagonistic effects may occur among the species, which may result in a great reduction or no effect on the soil or on the disease control effect.
In the growth process of peanuts, continuous cropping affects the high yield of peanuts, and continuous cropping obstacles are mainly reflected in the aspects of chemical sensitive toxic substance accumulation, soil microflora imbalance, enzyme activity reduction, nutrient imbalance and the like. In the aspect of relieving peanut continuous cropping, the endophyte has a remarkable effect, and documents about the application of peanut endophyte to a compound microbial fertilizer in peanut planting, in particular to the improvement of a continuous cropping soil microbial flora and the relief of peanut continuous cropping obstacle by compounding the endophyte with other microbial strains are rarely disclosed.
Disclosure of Invention
In order to solve the technical problems, the invention provides the seaweed compound microbial organic fertilizer containing the endophyte special for the peanuts, the organic combination of the organic fertilizer, the inorganic fertilizer and the biological fertilizer is realized, the nutrition is comprehensive and balanced, the survival capability of the endophyte is effectively improved by adding the seaweed, the nitrogen fixation capability of the endophyte can be effectively improved by the endophyte, the nitrogen content in the soil can be continuously increased, the occurrence of plant diseases and insect pests can be reduced, and the quality and the yield of the peanuts can be improved.
The organic fertilizer provided by the invention comprises the following raw materials in parts by weight:
the seaweed compound microbial organic fertilizer containing endophytes for peanuts is characterized by comprising the following raw materials in parts by weight:
400-600 parts of plant source organic matter, 300-400 parts of animal source organic matter, 100-200 parts of activated humic acid, 40-80 parts of medium trace elements, 10-20 parts of a nutrition regulator, 1-20 parts of a compound microbial agent containing endophytes, 15-20 parts of a seaweed extract and 5-10 parts of chitin.
The plant source organic matter is at least one of edible fungus waste residue, manioc waste, furfural residue, straw and cotton stalk.
The animal source organic matter is at least one of pig manure, cow manure, sheep manure, chicken manure and duck manure.
The activated humic acid is activated humic acid powder containing water-soluble yellow brown humic acid after being activated by sodium hydroxide and sodium sulfate.
In the medium trace elements, 30wt% of calcium chloride, 30wt% of ferrous sulfate, 15 wt% of magnesium sulfate, 10 wt% of zinc sulfate and 15 wt% of borax;
the nutritional regulator comprises at least one of vitamin C, citrate, gamma-aminobutyric acid, and 5-aminolevulinic acid.
The endophyte is separated from the root of the peanut and has the effects of fixing nitrogen and preventing bio-control;
the endophyte is Bacillus subtilis A-A1-1, A-A1-23, Bacillus amyloliquefaciens A-A5-7, A-A5-16, Bacillus thuringiensis A-A4-25.
The composite probiotic containing the endophyte comprises the endophyte separated from the root of peanut, wherein the total viable bacteria content is 500-1000 hundred million cfu/g; the microbial inoculum also comprises at least one of bacillus subtilis, bacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, lactobacillus plantarum, saccharomycetes, trichoderma harzianum, rhizoma nitrogen-fixing rhizobia, azospirillum brasilense, lactobacillus plantarum and streptomyces microflavus, and the compounding ratio of the two microbial inoculums is 3: 1.
The endophyte is prepared by performing liquid fermentation on each single strain, performing flocculation precipitation by adopting alginic acid and chitosan oligosaccharide, performing low-temperature vacuum drying and crushing, and mixing, wherein the water content is kept within 5 percent, and the number of effective viable bacteria is 500-1000 hundred million cfu/g.
The preparation method of the seaweed compound microbial organic fertilizer containing endophytes for peanuts comprises the following steps:
1) fully and uniformly mixing plant source organic matter, animal source organic matter, activated humic acid, medium trace elements, a nutrition regulator, a seaweed extract and chitin, and regulating the pH of the material to be between 5.5 and 8.0;
2) uniformly mixing the composite microbial powder containing endophytes with the material in the step (1), adding water, wherein the using amount of the water is 100 times of the weight of the bacterial powder, obtaining composite microbial liquid, adding 10-50 parts of seaweed extract, and uniformly stirring;
3) and (3) drying the material in the step (2) in vacuum at 40-60 ℃, and crushing to 40-80 meshes to obtain the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts.
Compared with the peanut microbial fertilizer mentioned in the background art, the peanut microbial fertilizer has the following obvious advantages:
(1) the compound microbial fertilizer containing the endophytic bacterial strains separated from the roots of the peanuts is adopted, and other endophytic bacteria are not adopted, so that the compound microbial fertilizer is more favorable for being colonized in the peanut plants, can survive and grow in the peanut plants, particularly in the root systems, and plays a role in promoting the growth; particularly, the obstacle of continuous cropping of peanuts can be effectively relieved in the aspects of improving the soil microflora and increasing the enzyme activity; in addition, the invention compounds endophyte with other strains, and is assisted by the nutrition effect and soil improvement effect of animal and plant source organic matters, so that the effects are increased, the viability of microorganisms is comprehensively improved, the fertilizer efficiency is improved, and the yield of crops is increased;
(2) the chitin is adopted, the greatest effect of the chitin is as a polysaccharide oligosaccharide plant immunity inducer, the plant autoimmune system can be activated to realize the disease-resistant effect, and the chitin oligosaccharide plant immunity inducer is non-toxic and harmless, so that the disease and insect resistance of peanut plants is reduced;
(3) compared with the urea sulfate microbial fertilizer special for peanuts mentioned in the background art, the microbial fertilizer contains activated humic acid, and the activated humic acid is water-soluble yellow-brown humic acid, has a good promotion effect on plant growth, and is harmless to the environment.
Detailed Description
The present invention will be further described with reference to specific examples so that those skilled in the art may better understand the present invention, but the present invention is not limited thereto.
Example 1
The seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following raw material components: 400 parts of plant source organic matter, 300 parts of animal source organic matter, 150 parts of activated humic acid, 40 parts of medium trace elements, 20 parts of a nutrition regulator, 16 parts of a compound microbial agent containing endophytes, 15 parts of a seaweed extract and 10 parts of chitin;
the plant source organic matter is specifically as follows: 40 parts of edible fungus waste residues, 120 parts of cassava residues, 80 parts of furfural residues, 120 parts of straws and 40 parts of cotton stalks;
animal-derived organic matter: 200 parts of pig manure and 100 parts of chicken manure;
activating humic acid: activated humic acid powder containing water-soluble yellow brown humic acid after being activated by sodium hydroxide and sodium sulfate is 150 parts;
medium trace elements: wherein, 30 percent of calcium chloride, 30 percent of ferrous sulfate, 15 percent of magnesium sulfate, 10 percent of zinc sulfate and 15 percent of borax;
the nutrition regulator is vitamin C, citrate, gamma-aminobutyric acid and 5-aminolevulinic acid in a weight ratio of 1:4:1: 1;
the composite probiotic agent containing endophyte comprises: 3 peanut endophytes, 3 bacillus subtilis, 3 bacillus laterosporus, 2 bacillus megaterium, 1 bacillus mucilaginosus, 1 lactobacillus plantarum, 1 saccharomycete and 2 trichoderma harzianum;
the endophytes comprise 1 part of bacillus subtilis A-A1-1, 1 part of bacillus amyloliquefaciens A-A5-7 and 1 part of bacillus thuringiensis A-A4-25;
the preparation method of the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following steps:
(1) fully and uniformly mixing plant source organic matter, animal source organic matter, activated humic acid, medium trace elements, a nutrition regulator, a seaweed extract and chitin powder in a stirrer, and regulating the pH of the materials to be about 6.5;
(2) and (3) drying the materials in the step (1) at the temperature of about 50 ℃, and crushing the materials to about 60 meshes to obtain the product.
Example 2
The seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following raw material components:
600 parts of plant source organic matter, 300 parts of animal source organic matter, 100 parts of activated humic acid, 55 parts of medium trace elements, 15 parts of citrate, 20 parts of compound microbial agent containing endophyte, 20 parts of seaweed extract and 8 parts of chitin, wherein in the plant source organic matter: 50 parts of edible fungus waste residues, 300 parts of cassava residues, 150 parts of furfural residues and 100 parts of straws;
animal-derived organic matter: 100 parts of cow dung and 200 parts of sheep dung;
activating humic acid: 100 parts of activated humic acid powder containing water-soluble yellow brown humic acid after being activated by sodium hydroxide and sodium sulfate;
the medium trace elements comprise: 35 percent of calcium chloride, 25 percent of ferrous sulfate, 15 percent of magnesium sulfate, 15 percent of zinc sulfate and 10 percent of borax.
The composite probiotic preparation: 4 parts of 3 peanut endophytes, 3 parts of bacillus subtilis, 3 parts of bacillus laterosporus, 5 parts of bacillus megaterium, 1 part of bacillus mucilaginosus, 1 part of lactobacillus plantarum, 2 parts of saccharomycetes and 1 part of trichoderma harzianum;
and (3) seaweed extract: 20 parts of (1);
chitin: 8 parts of a mixture;
the 3 peanut endophytes are respectively: 2 parts of bacillus subtilis A-A1-23, 1 part of bacillus amyloliquefaciens A-A5-16 and 1 part of bacillus thuringiensis A-A4-25;
the preparation method of the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following steps:
1) fully and uniformly mixing plant source organic matter, animal source organic matter, activated humic acid, medium trace elements, a nutrition regulator, a seaweed extract and chitin powder in a stirrer, and regulating the pH of the materials to be about 7.0;
2) drying the materials in the step 1) at 50 ℃, and crushing to 60 meshes to obtain the product.
Example 3
The seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following raw material components:
500 parts of plant source organic matter, 350 parts of animal source organic matter, 160 parts of activated humic acid, 60 parts of medium trace elements, 10 parts of citrate, 18 parts of compound microbial agent containing endophyte, 20 parts of seaweed extract and 8 parts of chitin, wherein in the plant source organic matter: 50 parts of edible fungus waste residues, 200 parts of cassava residues, 150 parts of furfural residues and 100 parts of straws;
animal-derived organic matter: 100 parts of cow dung and 250 parts of sheep dung;
activating humic acid: 160 parts of activated humic acid powder containing water-soluble yellow brown humic acid after being activated by sodium hydroxide and sodium sulfate;
the medium trace elements comprise: 25% of calcium chloride, 35% of ferrous sulfate, 10% of magnesium sulfate, 20% of zinc sulfate and 10% of borax.
The composite probiotic preparation: 4 parts of 3 peanut endophytes, 2 parts of bacillus subtilis, 2 parts of bacillus laterosporus, 4 parts of bacillus megaterium, 1 part of bacillus mucilaginosus, 1 part of lactobacillus plantarum, 2 parts of saccharomycetes and 2 parts of trichoderma harzianum;
and (3) seaweed extract: 20 parts of (1);
chitin: 8 parts of a mixture;
the 3 peanut endophytes are respectively: 2 parts of bacillus subtilis A-A1-23, 1 part of bacillus amyloliquefaciens A-A5-16 and 1 part of bacillus thuringiensis A-A4-25;
the preparation method of the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following steps:
1) fully and uniformly mixing plant source organic matter, animal source organic matter, activated humic acid, medium trace elements, a nutrition regulator, a seaweed extract and chitin powder in a stirrer, and regulating the pH of the materials to be about 7.0;
2) drying the materials in the step 1) at 50 ℃, and crushing to 60 meshes to obtain the product.
Comparative example 1
The difference from the embodiment 1 is that the bacterial manure is not adopted, but other raw materials in the embodiment 1 are adopted, specifically, the seaweed compound microbial organic fertilizer special for peanuts containing endophytes comprises the following raw material groups: 400 parts of plant source organic matter, 300 parts of animal source organic matter, 150 parts of activated humic acid, 40 parts of medium trace elements, 20 parts of nutrition regulator, 15 parts of seaweed extract and 10 parts of chitin. The rest is the same as in example 1.
Comparative example 2
The difference from the example 1 is that the adopted compound microbial agent is not a compound microbial agent containing endophyte, but does not contain the compound microbial agent containing endophyte, and the composition of the microbial agent is as follows: 4 parts of bacillus subtilis, 4 parts of bacillus laterosporus, 3 parts of bacillus megaterium, 1 part of bacillus mucilaginosus, 1 part of lactobacillus plantarum, 1 part of saccharomycete and 2 parts of trichoderma harzianum; the total amount is 16 parts.
Comparative example 3
The difference from the embodiment 1 is that the consumption of peanut endophytes is less, specifically: the composite probiotic agent containing endophyte comprises: 1 part of 3 peanut endophytes, 4 parts of bacillus subtilis, 4 parts of bacillus laterosporus, 2 parts of bacillus megaterium, 1 part of bacillus mucilaginosus, 1 part of lactobacillus plantarum, 1 part of saccharomycete and 2 parts of trichoderma harzianum;
the endophyte comprises 0.4 part of bacillus subtilis A-A1-1, 0.3 part of bacillus amyloliquefaciens A-A5-7 and 0.3 part of bacillus thuringiensis A-A4-25.
Comparative example 4
The difference from the embodiment 1 is that the peanut endophyte is used in a large amount, specifically: the composite probiotic agent containing endophyte comprises: 9 parts of 3 peanut endophytes, 1 part of bacillus subtilis, 1 part of bacillus laterosporus, 1 part of bacillus megaterium, 1 part of bacillus mucilaginosus, 1 part of lactobacillus plantarum, 1 part of saccharomycete and 1 part of trichoderma harzianum;
the endophytes comprise 1 part of bacillus subtilis A-A1-1, 1 part of bacillus amyloliquefaciens A-A5-7 and 1 part of bacillus thuringiensis A-A4-25.
Comparative example 5
The difference from example 1 is that only peanut endophytes were used, and no other complex microbial inoculum was used. Specifically, the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following raw material groups: 400 parts of plant source organic matter, 300 parts of animal source organic matter, 150 parts of activated humic acid, 40 parts of medium trace elements, 20 parts of a nutrition regulator, 16 parts of peanut endophytes, 15 parts of seaweed extract and 10 parts of chitin.
Comparative example 6
The difference from example 1 is that the amount of the complex microbial agent containing endophyte is far less than that of example 1, and is only 5 parts. The method comprises the following specific steps: the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following raw material components: 400 parts of plant source organic matter, 300 parts of animal source organic matter, 150 parts of activated humic acid, 40 parts of medium trace elements, 20 parts of a nutrition regulator, 5 parts of a compound microbial agent containing endophyte, 15 parts of a seaweed extract and 10 parts of chitin;
the composite probiotic agent containing endophyte comprises: 1 part of 3 peanut endophytes, 1 part of bacillus subtilis, 0.5 part of bacillus laterosporus, 0.5 part of bacillus megaterium, 0.5 part of bacillus mucilaginosus, 0.5 part of lactobacillus plantarum, 0.5 part of saccharomycete and 0.5 part of trichoderma harzianum;
the endophyte comprises 0.4 part of bacillus subtilis A-A1-1, 0.3 part of bacillus amyloliquefaciens A-A5-7 and 0.3 part of bacillus thuringiensis A-A4-25.
Comparative example 7
The difference from the example 1 is that the dosage of the compound microorganism bacterium agent containing endophyte is higher than that of the example 1, and reaches 30 parts. The method comprises the following specific steps: the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts comprises the following raw material components: 400 parts of plant source organic matter, 300 parts of animal source organic matter, 150 parts of activated humic acid, 40 parts of medium trace elements, 20 parts of a nutrition regulator, 30 parts of a compound microbial agent containing endophyte, 15 parts of a seaweed extract and 10 parts of chitin;
the composite probiotic agent containing endophyte comprises: 15 parts of 3 peanut endophytes, 2 parts of bacillus subtilis, 2 parts of bacillus laterosporus, 2 parts of bacillus megaterium, 2 parts of bacillus mucilaginosus, 2 parts of lactobacillus plantarum, 3 parts of saccharomycetes and 2 parts of trichoderma harzianum;
the endophytes comprise 5 parts of bacillus subtilis A-A1-1, 5 parts of bacillus amyloliquefaciens A-A5-7 and 5 parts of bacillus thuringiensis A-A4-25.
Example 4
The above examples 1, 2 and comparative examples were applied as follows, comparing to a common compound fertilizer:
the test group and the control group select the continuous cropping land of the peanuts for more than 3 years for carrying out the test. The control group is fertilized by adopting a common compound fertilizer, and the control group is fertilized by a peanut and seaweed compound microbial fertilizer containing endophyte, wherein the fertilizing amount is the same as that of the rest field management.
Regarding the emergence rate and the plant diseases and insect pests in the growth period of the peanuts, the inventor performs the following experiments:
TABLE 1 Main indices of the seedling stage and growth stage of peanut in the examples and comparative examples
As can be seen from Table 1, the control example applied a common fertilizer with a lower emergence rate than the examples, a higher death rate and a more severe root rot, and accompanied by the occurrence of soil insects, in addition to which the leaf spot number was higher.
The peanuts in the embodiments 1 to 3 have high emergence rate, less dead seedlings and less root rot and insect pests, which shows that the bacterial manure provided by the invention provides more appropriate nutrition for the growth of the peanuts and also has more remarkable disease and insect resistance.
In comparative example 1, the rate of emergence was higher than that of the comparative example, but significantly lower than that of each example; compared with the embodiment 1, the compound microbial fertilizer containing endophytes is not adopted, so that the compound microbial fertilizer containing endophytes has the effects of loosening soil and promoting the growth of peanuts; from the aspect of the pest and disease incidence rate of the comparative example 1, the adoption of the compound microbial agent containing endophyte also has remarkable capability of resisting pests and diseases;
in the comparative example 2, the adopted composite bacteria are microbial inoculums without peanut endophytes, and the result shows that the emergence rate is slightly higher than that of the comparative example 1, the incidence rate of root rot and other plant diseases and insect pests is slightly lower than that of the comparative example 1, but the growth condition of the peanuts in the comparative example 2 is still not as good as that of each example in terms of the whole growth indexes of the peanuts; the peanut endophyte has obvious effect in the compound microbial inoculum, and the peanut endophyte and the compound microbial inoculum generate synergistic effect and act on peanut plants together, so that the emergence rate of peanuts and the disease resistance of the peanuts can be improved.
In comparative example 3, peanut endophytes were reduced; in the comparative example 4, the consumption of peanut endophytes is increased, and the results show that the growth conditions of peanuts are not as good as those of the examples, which shows that only the microbial inoculum within the consumption range of the invention can lead the growth of peanut plants to reach a better state;
in comparative example 5, only peanut endophyte is adopted, and other compound bacteria agents are not adopted, and the results show that the emergence rate of peanuts is lower than that of the embodiment, and the disease and pest resistance is far lower than that of the embodiment, which shows that only peanut endophyte is adopted, the effect is relatively limited, and the growth of peanuts can achieve a good effect only by combining the peanut endophyte with other compound bacteria provided by the invention.
In comparative examples 6 and 7, the reduction or increase of the amount of the complex microbial inoculant containing endophytes can affect the growth of peanuts to a greater or lesser extent, or cause the emergence rate to be reduced, or cause the disease and pest resistance to be weakened.
TABLE 2 influence of microbial fertilizers on peanut yield (average value) in examples and comparative examples
As can be seen from the data in table 2, the number of pods is greater in the examples of the present invention than in the control examples, so that the plumpness is higher, and the fruit weight, kernel weight and yield are higher;
the results of the comparative example 1, in which no bacterial manure is used and only the other raw materials in the example 1 are used, show that the indexes of peanut yield, plumpness, fruit weight and the like are far inferior to those of the examples.
In comparative example 2, the compound microbial inoculum containing no endophyte is used, and the results show that the indexes of peanut yield, plumpness, fruit weight and the like are far inferior to those of the examples.
In comparative example 3, the consumption of peanut endophytes is reduced, in comparative example 4, the consumption of peanut endophytes is increased, in comparative example 5, only peanut endophytes are adopted, other compound microbial agents are not adopted, in comparative example 6, the consumption of compound microbial agents containing endophytes is reduced, and in comparative example 7, the consumption of compound microbial agents containing endophytes is increased, and the indexes of yield, plumpness, fruit weight and the like are far inferior to those of the examples. Therefore, after the dosage of endophyte or other microbial inoculum is adjusted, the yield of peanuts, the fruiting quantity and the weight of all fruits can be changed, which shows that the microorganism plays an important role in the growth of the peanuts.
Particularly, in comparative example 2, each index is far lower than that in each example, which shows that the peanut endophyte has a remarkable effect in the growth of peanuts. The peanut endophyte plays roles in fixing nitrogen, resisting diseases, promoting the peanut to absorb nutrient elements and the like in the growth process of the peanut. The intraradicular and rhizosphere of peanuts contain various bacteria, such as bacilli, including bacilli, like bacillus megaterium, bacillus pumilus, bacillus licheniformis, bacillus cereus, bacillus firmus, bacillus coagulans, bacillus sphaericus, bacillus mycoides, bacillus stearothermophilus; bacillus lentus and the like; also included are arthrobacter, corynebacterium, alcaligenes, and the like.
As can be summarized from table 1 above, the endophyte compound fertilizer prepared in examples 1 to 3 can significantly improve the growth characteristics of peanuts, make plants tall and big, have thick and strong stems, multiple branches, large leaf areas, and vigorous dry matter accumulation, and can enhance the disease resistance of peanuts, improve the yield, and improve the quality of peanuts.
Claims (10)
1. The seaweed compound microbial organic fertilizer containing endophytes for peanuts is characterized by comprising the following raw materials in parts by weight:
400-600 parts of plant source organic matter, 300-400 parts of animal source organic matter, 100-200 parts of activated humic acid, 40-80 parts of medium trace elements, 10-20 parts of a nutrition regulator, 1-20 parts of a compound microbial agent containing peanut endophytes, 15-20 parts of a seaweed extract and 5-10 parts of chitin.
2. The endophyte-containing seaweed compound microbial organic fertilizer for peanuts as claimed in claim 1, wherein the plant-derived organic matter is at least one of edible fungus waste residues, cassava residues, furfural residues, straws and cotton stalks.
3. The endophyte-containing seaweed compound microbial organic fertilizer for peanuts as claimed in claim 1, wherein the animal-derived organic matter is at least one of pig manure, cow manure, sheep manure, chicken manure and duck manure.
4. The endophyte-containing seaweed compound microbial organic fertilizer for peanuts as claimed in claim 1, wherein the activated humic acid is activated humic acid powder containing water-soluble yellow brown humic acid after being activated by sodium hydroxide and sodium sulfate.
5. The seaweed compound microbial organic fertilizer containing endophytes for peanuts as claimed in claim 1, wherein the medium trace elements comprise 30wt% of calcium chloride, 30wt% of ferrous sulfate, 15% of magnesium sulfate, 10% of zinc sulfate and 15% of borax;
the nutritional regulator comprises at least one of vitamin C, citrate, gamma-aminobutyric acid, and 5-aminolevulinic acid.
6. The seaweed compound microbial organic fertilizer containing endophytes for peanuts as claimed in claim 1, wherein the endophytes are endophytes which are separated from peanut roots and have rooting and biocontrol effects.
7. The seaweed compound microbial organic fertilizer containing endophytes for peanuts as claimed in claim 6, wherein the endophytes are bacillus subtilis A-A1-1, A-A1-23, bacillus amyloliquefaciens A-A5-7, A-A5-16, and bacillus thuringiensis A-A4-25.
8. The seaweed compound microbial organic fertilizer containing the endophyte for the peanuts as claimed in claim 7, wherein the compound probiotic containing the endophyte contains 500-1000 hundred million cfu/g of total viable bacteria, and the compound probiotic containing the endophyte comprises the endophyte separated from the roots of the peanuts; the microbial inoculum also comprises at least one of bacillus subtilis, bacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, lactobacillus plantarum, saccharomycetes, trichoderma harzianum, rhizoma nitrogen-fixing rhizobia, azospirillum brasilense, lactobacillus plantarum and streptomyces microflavus, and the compounding ratio of the two microbial inoculums is 3: 1.
9. The seaweed compound microbial organic fertilizer containing endophytes for peanuts as claimed in any one of claims 6 to 8, wherein the endophytes are prepared by performing liquid fermentation on each single strain, performing flocculation precipitation on alginic acid and chitosan oligosaccharide, performing low-temperature vacuum drying, crushing and mixing, wherein the water content is kept within 5%, and the number of effective viable bacteria is 500-1000 hundred million cfu/g.
10. The preparation method of the seaweed compound microbial organic fertilizer containing endophytes for peanuts as claimed in claim 1, comprising the following steps:
fully and uniformly mixing plant source organic matter, animal source organic matter, activated humic acid, medium trace elements, a nutrition regulator, a seaweed extract and chitin, and regulating the pH of the material to be between 5.5 and 8.0;
2) uniformly mixing the composite microbial powder containing endophytes with the material in the step (1), adding water, wherein the using amount of the water is 100 times of the weight of the bacterial powder, obtaining composite microbial liquid, adding 10-50 parts of seaweed extract, and uniformly stirring;
3) and (3) drying and drying the materials in the step (2) at 40-60 ℃ in vacuum, and crushing to 40-80 meshes to obtain the seaweed compound microbial organic fertilizer containing endophytes and specially used for peanuts.
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| CN110498697A (en) * | 2019-09-24 | 2019-11-26 | 韦国安 | One cultivates peanut bio-organic fertilizer special and its production method |
| CN112062645A (en) * | 2020-09-30 | 2020-12-11 | 河北省农林科学院石家庄果树研究所 | Fertilizer composition for reducing cherry fruit cracking rate and preparation method thereof |
| CN113277901A (en) * | 2021-06-29 | 2021-08-20 | 重庆市农业科学院 | Preparation method of full-nutrient microbial biocontrol organic fertilizer |
| CN114287446A (en) * | 2021-12-10 | 2022-04-08 | 海南大学 | Biological agent for inducing plants to prevent and treat fungal diseases and preparation method and application thereof |
| CN115299207A (en) * | 2022-08-05 | 2022-11-08 | 中国科学院南京土壤研究所 | Fast construction and long-acting retention method for growth-adaptive desalting fertile layer of heavy saline-alkali soil |
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| CN115299207A (en) * | 2022-08-05 | 2022-11-08 | 中国科学院南京土壤研究所 | Fast construction and long-acting retention method for growth-adaptive desalting fertile layer of heavy saline-alkali soil |
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