CN111357820A

CN111357820A - Modified milk powder containing MFGM and preparation method thereof

Info

Publication number: CN111357820A
Application number: CN202010213419.0A
Authority: CN
Inventors: 吴善艳
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-03-24
Filing date: 2020-03-24
Publication date: 2020-07-03
Anticipated expiration: 2040-03-24
Also published as: CN111357820B

Abstract

The invention relates to the technical field of dairy products, in particular to modified milk powder containing MFGM and a preparation method thereof, wherein the modified milk powder containing MFGM comprises the following components of organic whole milk powder, β -yeast glucan, MFGM, DHA, probiotics, lactoferrin, N-acetylneuraminic acid, honey, vegetable oil and plant homologous extract.

Description

Modified milk powder containing MFGM and preparation method thereof

Technical Field

The invention relates to the technical field of dairy products, in particular to modified milk powder containing MFGM and a preparation method thereof.

Background

The Milk Fat Globule Membrane, MFGM for short, is a Membrane protein that coats Fat droplets outside mammary glands during secretion, is a 3-layer Membrane structure composed of phospholipids, sphingomyelins (gangliosides, sphingomyelin, glycerophospholipids) and multiple proteins, and has a complex structure and unique components, wherein the structure contains more than one hundred proteins. The milk fat globule membrane is discovered as a brain nutrition breakthrough after DHA is relayed by breast milk, and through scientific verification, the milk fat globule membrane can help the brain development of babies, enables the brain reaction to be sensitive, and is also discovered to be beneficial to immunity and gastrointestinal health. Meanwhile, the safety and tolerability of MFGM has also been clinically validated according to basic studies on its components.

The Chinese patent application with the application publication number of CN 107156302A, the publication date of which is 09.15.2017, discloses infant formula milk powder containing milk fat globule membranes and a preparation method thereof, wherein the milk fat globule membranes are added into the infant formula milk powder, so that the nutritional ingredients of the formula milk powder, particularly the composition of fat globules, are closer to breast milk, the brain and nerve development of a newborn is promoted, and the cognitive ability and the anti-infection ability are improved.

In breast milk, the specific 3-layer membrane structure of the MFGM milk fat globule membrane enables the fat in the breast milk to coat the dispersed lipid in the milk, so that the lipid in the breast milk is uniformly dispersed in the water environment; however, in the artificially prepared modified milk powder, since MFGM is newly separated from fat, it is difficult to form effective and stable coatings on various lipid components in the modified milk powder by simply adding MFGM, and therefore, even if MFGM is added, the modified milk powder is likely to have the phenomena of uneven dispersion, delamination and caking during brewing, and the uneven dispersion of the milk powder may cause the problem of uneven absorption.

Disclosure of Invention

In order to solve the problems of uneven dispersion and layering in the existing milk powder added with milk fat globule membrane, the invention provides a modified milk powder containing MFGM, which comprises the following components of organic whole milk powder, β -yeast glucan, MFGM, DHA, probiotics, lactoferrin, N-acetylneuraminic acid, honey, vegetable oil and plant homologous extract.

In the technical scheme, the milk powder comprises 55-80 parts of organic whole milk powder, 12-18 parts of β -yeast glucan, 0.5-5 parts of MFGM, 0.05-1.5 parts of DHA, 0.05-2 parts of probiotics, 0.05-1.5 parts of lactoferrin, 0.05-1.0 part of N-acetylneuraminic acid, 5-15 parts of honey, 5-15 parts of vegetable oil and 5-15 parts of plant homologous extract.

In the technical solution, the vegetable oil is preferably olive oil or coconut oil, and the olive oil is rich in long-chain monounsaturated fatty acids.

In the technical scheme, the full-fat milk powder is organic full-fat milk powder.

In the technical aspect of the above technical solution, further, the preparation method of the plant homologous extract comprises:

pulverizing acerola cherry, adding food grade ethanol, leaching at pH of 8-10 and temperature of 75-80 deg.C, and performing solid-liquid separation; adding the separated liquid into olive oil, stirring and dispersing at 100-120 ℃, and performing spray drying to obtain solid powder A; the mass ratio of the separated liquid to the olive oil is 1-1.5: 0.8-1.

Adding 0.6-1.2 wt% of cellulase, 0.6-1.2 wt% of pectinase and 0.5-1.0 wt% of α -amylase into the residue after solid-liquid separation, carrying out enzymolysis for 2-6 hours at 35-45 ℃, carrying out decolorization treatment on coconut shell activated carbon at the pH of 4-6 and the temperature of 55-65 ℃, drying and crushing the solid obtained by removing the activated carbon, and carrying out 45-60-mesh sieve selection treatment to obtain solid powder B;

the powder A and the powder B are plant homologous extracts, and the ratio of the powder A to the powder B is 1: 5 to 20.

The acerola cherry powder can improve the taste of the milk powder, and also contains abundant vitamins and various nutrient substances. In the scheme, the suberone triterpene in the acerola cherry can be extracted by ethanol leaching at the temperature of 75-80 ℃, the reaction concentration is improved, the extracted substance reacts with olive oil under an alkaline condition at the temperature of 100-120 ℃, the olive oil contains abundant long-chain monounsaturated fatty acids, the substance obtained by the extraction of the long-chain unsaturated fatty acids and the suberone triterpene can be combined with glycolipid or membrane protein on the surface of MFGM and can be well dispersed in lipid, and the abundant alcohol groups in the suberone triterpene can be compatible in an aqueous solution, so that the milk powder has better dispersibility and compatibility.

The residues after solid-liquid separation can be subjected to enzymolysis to enable substances in pulp cells to be dissolved out more fully, and organic matter macromolecules in the pulp residues after enzymolysis can be subjected to enzymolysis to form monosaccharide, amino acid and micromolecule peptide chains so as to be better absorbed by a human body.

In the technical solution of the above aspect, the probiotic is at least one of HN001 and HN 019.

The present invention also provides a method for preparing a modified milk powder containing MFGM as described above, comprising the steps of:

step a, dissolving plant homologous extract powder A with purified water, adding MFGM, and mixing and stirring at 40-50 ℃ to form a mixture M1;

in this process, the main component in the powder a can be sufficiently attached to the MFGM surface;

b, mixing the organic whole milk powder with the plant homologous extract powder B, the mixture M1 and the N-acetylneuraminic acid, and stirring uniformly by adopting ultrasonic waves to form a mixture M2;

step c, adding purified water, honey, β -yeast glucan, DHA, probiotics and vegetable oil into the mixture M2, and dispersing by using a high-speed dispersion machine to form a mixture M3;

d, hydrolyzing lactoferrin and pepsin at the temperature of 35-40 ℃ and under the condition that the pH value is 2-4, drying a product obtained after hydrolysis, and adding the product into the mixture M3 to form a mixture M4;

step e, homogenizing the mixture M4;

f, after homogenizing, sterilizing at 80-88 ℃, and concentrating to 15-20 DEG Be;

and g, cooling, freeze-drying, crushing, and sieving by a 40-45-mesh sieve to obtain the modified milk powder containing the MFGM.

In the technical solution, further, in the step a, the ratio of the powder a to the purified water is 1: 1 to 1.2.

In the technical scheme, further, in the step c, the dispersing speed of the dispersing machine is 7000-8000 r/min.

In the technical scheme, in the step e, the homogenizing pressure is 16-20 Mpa.

The modified milk powder of the MFGM provided by the invention can obtain better dispersibility and compatibility by adding the MFGM in the whole milk powder and matching with the addition of other raw materials, thereby ensuring the nutrition balance of the modified milk powder and being beneficial to the absorption of a human body.

Preferably, the present invention can improve the dispersibility of MFGM in the formula powder in the raw material and the binding property with lipid substances by using plant homologous extracts in combination with a specific preparation process, and help the lipid raw material to be better dispersed and dissolved in an aqueous solution.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following description will clearly and completely describe the embodiments of the present invention, and obviously, the described embodiments are a part of the embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The present invention also provides a preparation example of a modified milk powder containing MFGM as shown below:

example 1

Comprises the following raw materials:

60 parts of organic whole milk powder, 12 parts of β -yeast glucan, 1.5 parts of MFGM, 0.5 part of DHA, 0.5 part of probiotics, 0.5 part of lactoferrin, 0.5 part of N-acetylneuraminic acid, 5 parts of honey, 11 parts of vegetable oil and 5 parts of plant homologous extract;

preparing a plant homologous extract:

crushing acerola cherry, adding food grade ethanol, leaching at the temperature of 75 ℃ and the pH of 8, and performing solid-liquid separation, wherein the mass ratio of the acerola cherry powder to the food grade ethanol is 1: 0.5; adding the separated liquid into olive oil, stirring and dispersing at 100 ℃, and then carrying out spray drying to obtain solid powder A, wherein the mass ratio of the separated liquid to the olive oil is 1.5: 0.8;

adding 0.8 wt% of cellulase, 0.8 wt% of pectinase and 0.5 wt% of α -amylase into the residue after solid-liquid separation, carrying out enzymolysis for 5 hours at 40 ℃, then carrying out decolorization treatment by using coconut shell activated carbon at the conditions of pH 4.5 and temperature 60 ℃, drying and crushing the solid obtained by removing the activated carbon, and then carrying out 45-mesh screening treatment to obtain solid powder B, wherein the ratio of the powder A to the powder B is 1: 10.

Preparation of a modified milk powder containing MFGM:

step a, dissolving plant homologous extract powder A with purified water, adding MFGM, mixing and stirring at 45 ℃ to form a mixture M1, wherein the ratio of the powder A to the purified water is 1: 1;

b, mixing the organic whole milk powder with the plant homologous extract powder B, the mixture M1 and the N-acetylneuraminic acid, and stirring uniformly by adopting ultrasonic waves for about 20min to form a mixture M2;

c, adding purified water, honey, β -yeast glucan, DHA, probiotics and vegetable oil into the mixture M2, and dispersing for 15min at 8000r/min by using a high-speed dispersion machine to form a mixture M3, wherein the purified water in the step is 100 parts;

d, hydrolyzing lactoferrin and pepsin at 37 ℃ and under the condition that the pH value is 3, drying a product obtained after hydrolysis, and adding the product into the mixture C to form a mixture M4;

step e, homogenizing the mixture M4 under the homogenizing pressure of 20 Mpa;

f, after homogenizing, sterilizing at 85 ℃, and concentrating to 15 degrees Be;

and step g, cooling, freeze-drying, crushing, and sieving with a 45-mesh sieve to obtain the modified milk powder containing MFGM.

Example 2

Comprises the following raw materials:

65 parts of organic whole milk powder, 15 parts of β -yeast glucan, 2 parts of MFGM, 0.8 part of DHA, 0.8 part of probiotics, 0.8 part of lactoferrin, 0.8 part of N-acetylneuraminic acid, 8 parts of honey, 15 parts of vegetable oil and 8 parts of plant homologous extract;

preparing a plant homologous extract:

crushing acerola cherry, adding food grade ethanol, leaching, performing solid-liquid separation after leaching at the pH value of 8 and the temperature of 75 ℃, wherein the mass ratio of acerola cherry powder to food grade ethanol is 1: 0.5; adding the separated liquid into olive oil, stirring and dispersing at 100 ℃, and then carrying out spray drying to obtain solid powder A, wherein the mass ratio of the separated liquid to the olive oil is 1.5: 0.8;

adding 1.0 wt% of cellulase, 1.0 wt% of pectinase and 0.8 wt% of α -amylase into the residue after solid-liquid separation, carrying out enzymolysis for 5 hours at 40 ℃, then carrying out decolorization treatment by using coconut shell activated carbon at the conditions of pH 4.5 and temperature 60 ℃, drying and crushing the solid obtained by removing the activated carbon, and then carrying out 45-mesh screening treatment to obtain solid powder B;

the ratio of the powder A to the powder B is 1: 14.

preparation of a modified milk powder containing MFGM:

step a, dissolving plant homologous extract powder A with purified water, adding MFGM, mixing and stirring at 45 ℃ to form a mixture M1, wherein the ratio of the powder A to the purified water is 1: 1.2.

c, adding purified water, honey, β -yeast glucan, DHA, probiotics and vegetable oil into the mixture M2, and dispersing for 15min at 8000r/min by using a high-speed dispersion machine to form a mixture M3, wherein the purified water in the step is 110 parts;

step e, homogenizing the mixture M4 under the homogenizing pressure of 20 Mpa;

Example 3

Comprises the following raw materials:

75 parts of organic whole milk powder, 18 parts of β -yeast glucan, 2.5 parts of MFGM, 1.0 part of DHA, 1.0 part of probiotics, 1.0 part of lactoferrin, 1.0 part of N-acetylneuraminic acid, 8 parts of honey, 15 parts of vegetable oil and 10 parts of plant homologous extract;

preparing a plant homologous extract:

crushing acerola cherry, adding food grade ethanol, leaching, performing solid-liquid separation after leaching at the pH value of 8 and the temperature of 75 ℃, wherein the mass ratio of acerola cherry powder to food grade ethanol is 1: 0.5; adding the separated liquid into olive oil, stirring and dispersing at 100 ℃, and performing spray drying to obtain solid powder A, wherein the mass ratio of the separated liquid to the olive oil to the honey is 1.5: 0.8;

the ratio of the powder A to the powder B is 1: 14.

preparation of a modified milk powder containing MFGM:

step a, dissolving the plant homologous extract with purified water, adding MFGM, mixing and stirring at 40-50 ℃ to form a mixture M1, wherein the ratio of the powder A to the purified water is 1: 1;

c, adding purified water, honey, β -yeast glucan, DHA, probiotics and vegetable oil into the mixture M2, and dispersing for 15min at 8000r/min by using a high-speed dispersion machine to form a mixture M3, wherein the purified water in the step is 120 parts;

step e, homogenizing the mixture M4 under the homogenizing pressure of 20 Mpa;

The milk powder prepared in the above embodiment is tested by using GB 19644-2010 food safety standard milk powder, and the test result is as follows:

TABLE 1

The present invention also provides the following comparative examples

Comparative example 1

Comprises the following raw materials:

60 parts of organic whole milk powder, 12 parts of β -yeast glucan, 1.5 parts of MFGM, 0.5 part of DHA, 0.5 part of probiotics, 0.5 part of lactoferrin, 0.5 part of N-acetylneuraminic acid, 5 parts of honey, 8 parts of vegetable oil and 11 parts of acerola;

cleaning acerola cherry, directly drying and crushing, and screening by a 45-mesh sieve;

dissolving pulverized acerola powder A with purified water, adding MFGM, and mixing and stirring at 45 deg.C to obtain mixture M1;

mixing the organic whole milk powder with the mixture M1 and N-acetylneuraminic acid, and stirring by ultrasonic wave for about 20min to be uniform to form a mixture M2;

the remaining steps were in accordance with example 1.

Comparative examples 2,

On the basis of example 1, acerola cherries were changed to apples, and the remaining steps were the same as in example 1.

Comparative examples 3,

On the basis of example 1, olive oil was changed to peanut oil, and the rest of the procedure was the same as in example 1.

Comparative example 4

On the basis of the embodiment 1, the residue of the ground acerola cherry is not subjected to enzymolysis, so that solid powder B is obtained; the rest of the procedure was the same as in example 1.

Comparative example 5

Patent application CN 110178902 a modified milk powder obtained in example 1.

The following tests were carried out on the modified milk powders prepared in examples 1 to 3 of the present invention and the samples obtained in comparative examples 1 to 5;

test one, now put 45ml of water at different temperatures into the same transparent container, add 5g of sample, observe the time for the sample to naturally and completely dissolve (based on no occurrence of solid particles) without any shaking or other physical agitation, and the test results are shown in the following table:

TABLE 2

Temperature of water

Example 1

Example 2

Example 3

Comparative example 1

Comparative example 2

Comparative example 3

Comparative example 4

Comparative example 5

25℃

1min15s

1min10s

1min03s

Greater than 5min

4min18s

4min04s

30℃

53s

50s

45s

Greater than 5min

4min20s

4min05s

3min45s

35℃

42s

35s

30s

4min50s

4min43s

4min02s

3min49s

3min40s

40℃

32s

30s

28s

4min24s

4min28s

3min57s

3min36s

3min26s

45℃

25s

19s

15s

3min58s

4min15s

3min34s

3min18s

3min05s

50℃

12s

10s

9s

3min28s

3min53s

3min16s

2min51s

2min36s

55℃

10s

8s

7s

2min39s

3min17s

2min52s

2min43s

2min35s

Through the tests, the milk powder provided by the invention can be more easily dispersed in the water solution through a specific technical scheme, and compared with the existing full-cream milk powder, the water-soluble full-cream milk powder has more excellent solubility.

And secondly, placing 45ml of water at 30 ℃ into the same transparent container, adding 5g of sample, shaking up and down for 5s at the same amplitude, and observing the dissolution degree of the fixed particles in the transparent container, wherein the test results are shown in the following table:

TABLE 3

Test sample	Degree of dispersion of solid particles
		Example 1	The solid particles are completely dissolved, the solution is uniform and has no bubbles
Example 2	The solid particles are completely dissolved, the solution is uniform and has no bubbles
		Example 3	The solid particles are completely dissolved, the solution is uniform and has no bubbles
Comparative example 1	About 50% solids particle fractionThe presence of lumps, lipid stratification observed, small bubbles
		Comparative example 2	About 50% of the solid particles are partially agglomerated and lipid stratification is observed, with a small amount of bubbles being generated
Comparative example 3	About 40% of the solid particles are present partly agglomerated and lipid stratification, bubble generation, can be observed
		Comparative example 4	The part of about 40 percent of solid particles is agglomerated, the solution part is more uniform, and a small amount of bubbles are generated

According to the test, the milk powder provided by the invention can be quickly dissolved in normal-temperature water through a specific technical scheme, has better water-milk dispersibility, also overcomes the problem that the existing milk powder is easy to foam in the brewing process, and does not need to worry about the problems that the milk powder cannot be easily dispersed due to insufficient water temperature and the nutrition in the milk powder is damaged due to overhigh temperature.

And step three, placing 45ml of water at 40 ℃ into the same transparent container, adding 5g of sample, stirring until the sample is completely dissolved, standing at normal temperature for 2.5 hours, and observing the layering condition of the solution, wherein the test results are shown in the following table:

TABLE 4

Test sample	Degree of dispersion of solid particles
		Example 1	The solution is uniform and has no obvious layering
Example 2	The solution is uniform and has no obvious layering
		Example 3	The solution is uniform and has no obvious layering
Comparative example 1	Visible lipid stratification
		Comparative example 2	Precipitation at the bottom, lipid stratification was visible
Comparative example 3	Precipitation at the bottom, lipid stratification was visible
		Comparative example 4	More solid precipitate at the bottom without obvious stratification

Through the test experiments, the prepared milk powder provided by the invention has excellent stability after being dispersed in water, and the stable coating of the MFGM on the lipid in the milk powder is proved.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A modified milk powder containing MFGM is characterized by comprising the following raw materials of whole milk powder, β -yeast glucan, MFGM, DHA, probiotics, lactoferrin, N-acetylneuraminic acid, honey, vegetable oil and plant homologous extract.

2. The modified milk powder containing MFGM of claim 1, comprising 55-80 parts of organic whole milk powder, 12-18 parts of β -yeast glucan, 0.5-5 parts of MFGM, 0.05-1.5 parts of DHA, 0.05-2 parts of probiotics, 0.05-1.5 parts of lactoferrin, 0.05-1.0 parts of N-acetylneuraminic acid, 5-15 parts of honey, 5-15 parts of vegetable oil and 5-15 parts of plant homologous extract.

3. The MFGM-containing reconstituted milk powder of claim 1, wherein: the vegetable oil is olive oil or coconut oil, preferably olive oil.

4. The MFGM-containing reconstituted milk powder of claim 1, wherein: the whole milk powder is organic whole milk powder.

5. The formulated milk powder containing MFGM according to claim 1 or 2, characterized in that: the preparation method of the plant homologous extract comprises the following steps:

pulverizing acerola cherry, adding food grade ethanol, leaching at pH of 8-10 and temperature of 75-80 deg.C, and performing solid-liquid separation; adding the separated liquid into olive oil, stirring and dispersing at 100-120 ℃, and performing spray drying to obtain solid powder A;

6. The MFGM-containing reconstituted milk powder of claim 1, wherein: the probiotics is at least one of HN001 and HN 019.

7. The method for producing a modified milk powder containing MFGM according to any one of claims 1 to 6, comprising the steps of:

step e, homogenizing the mixture M4;

8. The MFGM-containing reconstituted milk powder of claim 1, wherein: in the step a, the ratio of the powder A to the purified water is 1: 1 to 1.2.

9. The MFGM-containing reconstituted milk powder of claim 1, wherein: in the step c, the dispersing speed of the dispersing machine is 7000-8000 r/min.

10. The MFGM-containing reconstituted milk powder of claim 1, wherein: in the step e, the homogenizing pressure is 16-20 Mpa.