CN111349181A - Pachymaran extraction method, pachymaran extract and poria product combined production process - Google Patents
Pachymaran extraction method, pachymaran extract and poria product combined production process Download PDFInfo
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- CN111349181A CN111349181A CN202010257579.5A CN202010257579A CN111349181A CN 111349181 A CN111349181 A CN 111349181A CN 202010257579 A CN202010257579 A CN 202010257579A CN 111349181 A CN111349181 A CN 111349181A
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- pachyman
- poria
- poria cocos
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention relates to a pachyman extraction method, a pachyman extract and a combined production process of a pachyman product, wherein the pachyman extraction method comprises the following steps: providing a crude tuckahoe product; micronizing Poria crude product to obtain Poria wall-broken powder; mixing the Poria wall-broken powder with water, and grinding to obtain Poria emulsion; carrying out enzymolysis on the poria cocos emulsion by adopting glucan hydrolase to prepare an enzymolysis liquid; deactivating the enzymolysis solution, performing solid-liquid separation, and collecting filtrate to obtain pachyman extract. The extraction method of pachyman can effectively avoid the use of organic solvent and improve the safety of the product on the basis of ensuring the extraction rate.
Description
Technical Field
The invention relates to the technical field of natural product extraction, in particular to a pachyman extraction method, a pachyman extract and a poria product combined production process.
Background
Poria is dried sclerotium of Wolf of Polyporaceae fungus Poria Poriacocos (Schw.) and has sweet, light and neutral taste, has effects of promoting diuresis, eliminating dampness, invigorating spleen, calming heart, etc., and can be used for treating edema, oliguria, phlegm retention, dizziness, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, insomnia, etc. Poria cocos is one of the four monarch eight-treasure in traditional Chinese medicines in China, is listed as the superior product from the classic 'Shennong Ben Cao Jing', is a large variety of traditional Chinese medicines commonly used in China as both medicine and food, is an important traditional Chinese medicine of a plurality of traditional Chinese medicine formulas and Chinese patent medicine compatibility formulas, creates forty formulas by taking Poria cocos as the main part in famous medical science Zhongjing of Han dynasty recorded in the 'golden plaque Yao Chong Ji' selection, and also has statistics to show that in 158 traditional Chinese medicines, the weight of Poria cocos accounts for 80%, while in 2015 edition 'Chinese pharmacopoeia' the adult formula preparations containing Poria cocos have 229 types, such as six-ingredient rehmannia pills, wrinkled Gianthyssop vital essence water and the like, and the use frequency and the use amount of Poria cocos are not declined for a long time.
The modern clinical and pharmacological research shows that pachyman has the functions of protecting liver, enhancing immunity, tranquilizing, resisting senility, reducing blood sugar, resisting mutagenesis, resisting tumor, etc., especially has excellent antitumor function, and the sugar has the functions of enhancing cell toxicity of macrophage and T lymphocyte, enhancing cell immunoreaction and activating body's immune monitoring system to tumor, and has mechanism related to activating complement.
Disclosure of Invention
Based on the above, there is a need for a pachyman extraction method, a pachyman extract and a poria product combined production process. The extraction method of pachyman can effectively avoid the use of organic solvent and improve the safety of the product on the basis of ensuring the extraction rate.
A pachyman extraction method comprises the following steps:
providing a crude tuckahoe product;
micronizing the crude product of Poria to obtain Poria wall-broken powder;
mixing the poria cocos wall-broken powder with water, and grinding to prepare a poria cocos emulsion;
carrying out enzymolysis on the poria cocos emulsion by adopting glucan hydrolase to prepare an enzymolysis liquid;
deactivating the enzymolysis solution, performing solid-liquid separation, and collecting filtrate to obtain pachyman extract.
In one embodiment, the step of providing crude poria cocos comprises the following steps:
decocting Poria with water, and collecting residue;
and drying the dregs to obtain the crude poria cocos product.
In one embodiment, the drying temperature of the medicine dregs is 50-80 ℃.
In one embodiment, the step of micronizing the crude poria cocos product comprises the following steps:
primarily crushing the crude poria cocos product, and sieving the crude poria cocos product with a screen of 40-200 meshes to obtain a crushed poria cocos product;
and carrying out superfine grinding on the poria cocos crushed material, and sieving the crushed material by using a sieve with more than 200 meshes to obtain the poria cocos wall-broken powder.
In one embodiment, the step of mixing the poria cocos wall-broken powder with water and grinding comprises the following steps:
mixing the poria cocos wall-broken powder and water in a mass ratio of 1: (2-8) mixing and grinding for 1-8 h.
In one embodiment, the glucan hydrolase is β - (1 → 3) glucan hydrolase.
In one embodiment, the concentration of the β - (1 → 3) glucan hydrolase in the enzymolysis liquid is 1% -3%, the enzymolysis temperature is 40-60 ℃, the enzymolysis time is 4-8 h, and the pH value of the enzymolysis liquid is 3-6.
In one embodiment, the step after collecting the filtrate further comprises the following steps:
concentrating the filtrate at the temperature of 50-85 ℃ to 10-20% of the volume of the filtrate to obtain a concentrated solution;
and drying the concentrated solution by adopting a spray drying method, wherein the drying conditions of the spray drying are that the air inlet temperature is 160-180 ℃, and the air outlet temperature is 65-85 ℃.
The pachyman extract obtained by the extraction method of pachyman.
A combined production process of Poria cocos products comprises the following steps:
decocting Poria with water to obtain decoction and residue;
concentrating, drying and granulating the decoction to prepare the poria cocos traditional Chinese medicine formula granules;
drying the dregs to obtain a crude tuckahoe product;
micronizing the crude product of Poria to obtain Poria wall-broken powder;
mixing the poria cocos wall-broken powder with water, and grinding to prepare a poria cocos emulsion;
carrying out enzymolysis on the poria cocos emulsion by adopting glucan hydrolase to prepare an enzymolysis liquid;
deactivating the enzymolysis solution, performing solid-liquid separation, collecting filtrate, concentrating, drying, and granulating to obtain pachyman solid granule.
The extraction method of pachyman adopts superfine pulverizing wall-breaking technology to break the barrier of plant cell wall, and form superfine wall-breaking powder with large specific surface area, then adopts colloid grinding emulsification technology to form uniformly dispersed emulsion, and adopts special hydrolysis technology of glucan hydrolase to reduce molecular weight of pachyman, and under the synergistic action of all technologies, the extraction rate of water-soluble pachyman can be effectively raised. The extraction method can effectively avoid the use of organic solvents by combining physical treatment and biodegradation, does not need inorganic salt or acid-base treatment, greatly reduces the process difficulty, reduces the production cost, ensures that the obtained product is more green and safe, and can meet the requirements of modern industrial production.
Detailed Description
In order that the invention may be more fully understood, a more particular description of the invention will now be rendered by reference to specific embodiments thereof that are illustrated in the appended drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
One embodiment of the invention provides a pachyman extraction method, which comprises the following steps:
s101: providing a crude tuckahoe product;
it can be understood that the crude poria cocos product in step S101 refers to a poria cocos-containing Chinese herbal medicine, which may be a pure natural unprocessed or simply processed crude drug, or may be decoction dregs, and preferably, the decoction dregs are used to extract pachyman, specifically: decocting Poria with water, and collecting residue; and drying the dregs to prepare a crude tuckahoe product for the subsequent steps. Furthermore, the drying temperature of the medicine dregs is 50-80 ℃ to ensure the drying effect and avoid the inactivation of the active components.
The pachyman in the poria cocos is macromolecular glucan with a main chain of β - (1 → 3) structure and β - (1 → 6) branched chains, and is not water-soluble, so that decocted medicine dregs contain a large amount of pachyman, the decocted medicine dregs are dried to serve as crude poria cocos products, the purpose of secondary utilization of resources is achieved, the production cost can be effectively saved, resource waste is avoided, water-soluble components enter decoction liquid through first decoction, the interference of the water-soluble components can be reduced, the water extraction impurity removal pretreatment steps in most pachyman preparation processes are reduced, the operation steps are simplified, the production efficiency is further improved, and the cost is reduced.
In addition, the extraction of pachyman from the decocted medicine residue can be combined with the production process for preparing the tuckahoe formulation granule, the decoction is used for preparing the traditional Chinese medicine formulation granule, and the residue left after decoction is used for further extracting the pachyman, so that the reasonable allocation and utilization of resources are realized, and the method has great significance in industrial production.
S102: micronizing Poria crude product to obtain Poria wall-broken powder;
the technical personnel of the invention find through research that one of the reasons for poor water solubility of pachyman is limited by plant cell walls, common water decoction is difficult to dissolve out, if organic solvent is adopted for extraction, the difficulty of post-treatment is increased, and the safety of the product is reduced, so that the superfine grinding treatment can be carried out firstly, and then the subsequent steps are carried out, thereby not only improving the efficiency of the subsequent treatment and the dissolution rate of the polysaccharide, but also effectively avoiding the use of organic solvent and the like, reducing the production cost, having higher environmental protection effect and reducing the harm to operators.
Further, step S102 includes the steps of:
s1021: primarily crushing the crude poria cocos product, and sieving the crude poria cocos product with a screen of 40-200 meshes to obtain a crushed poria cocos product;
the crushing treatment is carried out firstly, and then the subsequent superfine crushing is carried out, so that the burden of subsequent superfine crushing equipment can be reduced, the superfine crushing effect is improved, and the uniformity of the particle size of the crushed material can be improved.
Further, it is preferable that the powder is passed through a screen of 80 to 120 meshes after the preliminary pulverization in the step S1021.
In step S1021, the pulverization may be performed by using an existing pulverization device, such as a universal pulverizer.
S1022: micronizing Poria, and sieving with 200 mesh sieve to obtain Poria cell wall-broken powder.
In step S1022, conventional micronizing equipment may be used for pulverization, and is not particularly limited herein, and should be understood to fall within the scope of the present invention. Further, the poria cocos polysaccharide is preferably subjected to superfine grinding and then sieved by a screen mesh of 300-350 meshes, so that the subsequent steps can be favorably carried out, and the dissolution rate of the pachymaran can be further improved.
S103, mixing the poria cocos wall-breaking powder with water, and grinding to prepare a poria cocos emulsion;
the pachyman is further exposed by further grinding the tuckahoe broken wall powder, so that the hydrolysis effect of special enzyme is improved, and the extraction rate of pachyman is improved.
Further, the specific operation of step S103 is: mixing the poria cocos wall-broken powder and water in a mass ratio of 1: (2-8) mixing and grinding for 1-8 h. Furthermore, the grinding time is 1-2 h. Further, the grinding device may be a colloid mill.
S104: and carrying out enzymolysis on the poria cocos emulsion by adopting glucan hydrolase to prepare an enzymolysis liquid.
The molecular weight of pachyman is reduced, the water solubility of pachyman is improved, and effective components are effectively dissolved out by adopting glucan hydrolase to carry out enzymolysis on the pachyman.
Further, it is preferable to use β - (1 → 3) glucan hydrolase, which is a macromolecular glucan having a main chain of β - (1 → 3) structure and having β - (1 → 6) branches, and to enzymatically hydrolyze sugar chains of pachyman by using β - (1 → 3) glucan hydrolase having a strong specificity, so that the water solubility of the polysaccharide can be improved, and the extraction rate of pachyman can be effectively improved.
Further, the concentration of β - (1 → 3) glucan hydrolase in the enzymolysis liquid is 1% -3%;
further, the enzymolysis temperature is 40-60 ℃, the enzymolysis time is 4-8 h, and the pH value of the enzymolysis liquid is 3-6.
S105: deactivating the enzymolysis liquid, performing solid-liquid separation, and collecting filtrate, wherein the filtrate is pachyman extract.
Further, the step of deactivating in step S105 includes the steps of: and heating, decocting and deactivating the enzymolysis liquid, preferably boiling and preserving heat for 5-10 minutes.
Further, the solid-liquid separation method in step S105 may be an existing solid-liquid separation method, which is not particularly limited herein, and preferably a centrifugation method, and may be one or more of conventional centrifuges for production such as a three-leg centrifuge, a horizontal screw centrifuge, a butterfly centrifuge, or a tube centrifuge, and may be operated according to the normal operation parameters of each apparatus.
It should be noted that the pachyman extract of the present invention can be prepared into various dosage forms, such as: liquid preparations (such as oral liquid), paste preparations (such as tuckahoe paste), tablets, pills and the like can be prepared by the method, and the preparation is understood to be in the protection scope of the invention. Therefore, when a liquid preparation is required, the extract obtained by the extraction in step S105 can be directly used, and is subjected to conventional post-treatment, such as concentration to a desired concentration, mixing with other functional reagents, addition of corresponding pharmaceutically acceptable excipients, preparation, sterilization or packaging, and the like, and preferably prepared into a solid preparation by the following steps:
concentrating the filtrate at 50-85 deg.c to 10-20 vol% to obtain concentrated solution; drying the concentrated solution by adopting a spray drying method, wherein the drying conditions of the spray drying are that the air inlet temperature is 160-180 ℃, and the air outlet temperature is 65-85 ℃.
The extraction method of pachyman comprises breaking the barrier of plant cell wall by superfine pulverizing wall-breaking technique, forming ultramicron with large specific surface area, colloid grinding and emulsifying to obtain uniform suspension, reducing molecular weight of pachyman by hydrolysis technique specific to glucan hydrolase, and effectively increasing extraction rate of water-soluble pachyman under synergistic effect of each technique. The extraction method can effectively avoid the use of organic solvents by combining physical treatment and biodegradation, and does not need inorganic salt or acid-base treatment, thereby greatly improving and reducing the process difficulty and the generation cost, and the obtained product is more green and safe and can meet the requirements of modern industrial production.
The invention also provides a pachyman extract obtained by the extraction method of pachyman.
The pachyman extraction method is as described above, and is not described herein again. The pachyman extract obtained by extraction may be prepared into various dosage forms by conventional preparation methods as required, and is not particularly limited herein.
The invention also provides a combined production process of the poria cocos product, which comprises the following steps:
s201: decocting Poria with water to obtain decoction and residue;
s202: concentrating the decoction, drying, and granulating to obtain Poria granule;
s203: drying the residue to obtain Poria crude product;
s204: micronizing Poria crude product to obtain Poria wall-broken powder;
s205: mixing the Poria wall-broken powder with water, and grinding to obtain Poria emulsion;
s206: carrying out enzymolysis on the poria cocos emulsion by adopting glucan hydrolase to prepare an enzymolysis liquid;
s207: deactivating the enzymolysis solution, performing solid-liquid separation, collecting filtrate, concentrating, drying, and granulating to obtain pachyman solid granule.
Wherein, the step S201 may be performed by decocting with an existing method, and the step S202 may be performed by preparing the traditional Chinese medicine formula granule with an existing method, which is not particularly limited herein; steps S203 to S207 are as described above, and are not described herein again.
The extraction of pachymaran and the production process of the tuckahoe formulation granule are combined, the decoction is used for preparing the traditional Chinese medicine formulation granule, and the residue left after decoction is used for further extracting the pachymaran, so that the effective utilization of resources is realized, the production efficiency can be effectively improved, the production cost is reduced, and the method has great significance in industrial production.
The present invention will be described below with reference to specific examples.
Example 1
The extraction method of pachyman extract of the present embodiment comprises the following steps:
(1) taking a proper amount of the tuckahoe coarse particles after being boiled with water, and drying at 70 ℃ to obtain clean tuckahoe coarse particles;
(2) taking 10kg of clean poria cocos coarse particles, putting the clean poria cocos coarse particles into a 40B type universal grinder for grinding, filtering the clean poria cocos coarse particles by using an 80-mesh vibrating screen to collect powder, breaking the walls by using an AB06 type jet mill, and filtering and collecting the broken walls by using a 300-mesh vibrating screen;
(3) adding 4 times of water (mass ratio of wall-broken powder to water is 1:4) into the wall-broken powder, stirring, mixing, grinding in a JMS-80B type colloid grinder for 1.5 hours, and adding β - (1 → 3) glucan hydrolase in an amount of 2% (w/w), at 51 deg.C and pH of 4.0, stirring, and performing enzymolysis for 4 hours;
(4) heating, decocting and deactivating the enzymolysis solution to boiling, keeping the temperature for 10 minutes, centrifuging at 3000r/min for 30 minutes, performing suction filtration with 350 meshes to obtain pachyman filtrate, and preparing into pachyman oral liquid according to the requirements of the preparation.
Example 2
The extraction method of pachyman extract of the present embodiment comprises the following steps:
(1) taking a proper amount of the tuckahoe coarse particles after being boiled with water, and drying at 75 ℃ to obtain clean tuckahoe coarse particles;
(2) taking 10kg of clean poria cocos coarse particles, putting the clean poria cocos coarse particles into a 40B type universal grinder for grinding, filtering the clean poria cocos coarse particles by using a 100-mesh vibrating screen to collect powder, breaking the walls by using an AB06 type jet mill, and filtering and collecting the broken walls by using a 350-mesh vibrating screen;
(3) adding 4 times of water (mass ratio of wall-broken powder to water is 1:4) into the wall-broken powder, stirring, mixing, grinding in JMS-80B colloid mill for 1 hr, adding β - (1 → 3) glucan hydrolase in an amount of 1% (w/w), at 43 deg.C and pH of 4.3, stirring, and performing enzymolysis for 6 hr
(4) Heating, decocting and deactivating the enzymolysis solution to boiling, keeping the temperature for 10 minutes, centrifuging at 5000r/min for 20 minutes, performing suction filtration with 300 meshes to obtain pachyman filtrate, and preparing into oral mixture according to the requirements of preparation.
Example 3
The extraction method of pachyman extract of the present embodiment comprises the following steps:
(1) taking a proper amount of the tuckahoe coarse particles after being boiled with water, and drying at 80 ℃ to obtain clean tuckahoe coarse particles;
(2) taking 10kg of clean poria cocos coarse particles, putting the clean poria cocos coarse particles into a 40B type universal grinder for grinding, filtering the clean poria cocos coarse particles by using an 80-mesh vibrating screen to collect powder, breaking the walls by using an AB06 type jet mill, and filtering and collecting the broken walls by using a 300-mesh vibrating screen;
(3) adding 6 times of water (mass ratio of wall-broken powder to water is 1:6), stirring, mixing, grinding in JMS-80B colloid mill for 2 hr, adding β - (1 → 3) glucan hydrolase in an amount of 1.5% (w/w), at 48 deg.C and pH of 4.6, stirring, and performing enzymolysis for 5 hr
(4) Heating, decocting and deactivating the enzymolysis liquid to boiling, keeping the temperature for 5 minutes, centrifuging at 4000r/min for 30 minutes, and performing suction filtration with 300 meshes to obtain pachyman filtrate.
(6) Concentrating pachyman filtrate at 75 deg.C under reduced pressure to 16% of original volume, spray drying the concentrated solution at air inlet temperature of 170 deg.C and air outlet temperature of 76 deg.C to obtain pachyman powder, and making into pachyman solid preparation according to the requirements of preparation.
Comparative example 1
The extraction process of the pachyman extract of this comparative example was essentially the same as that of example 3, except that the enzymatic hydrolysis and deactivation steps were omitted, specifically:
(1) taking a proper amount of the tuckahoe coarse particles after being boiled with water, and drying at 80 ℃ to obtain clean tuckahoe coarse particles;
(2) taking 10kg of clean poria cocos coarse particles, putting the clean poria cocos coarse particles into a 40B type universal grinder for grinding, filtering the clean poria cocos coarse particles by using an 80-mesh vibrating screen to collect powder, breaking the walls by using an AB06 type jet mill, and filtering and collecting the broken walls by using a 300-mesh vibrating screen;
(3) adding 6 times of water (mass ratio of wall-broken powder to water is 1:6) into the wall-broken powder, stirring, mixing, and grinding in JMS-80B colloid grinder for 2 hr to obtain Poria emulsion;
(4) and directly centrifuging the emulsion for 30 minutes at 4000r/min, and performing suction filtration with 300 meshes to obtain pachyman filtrate.
(5) Concentrating pachyman filtrate at 75 deg.C under reduced pressure to 15% of original volume, spray drying the concentrated solution at air inlet temperature of 171 deg.C and air outlet temperature of 76 deg.C to obtain pachyman powder, and making into pachyman solid preparation according to the requirements of preparation.
Comparative example 2
The extraction process of the pachyman extract of this comparative example was essentially the same as example 3, except that the grinding emulsification step was omitted, specifically:
(1) taking a proper amount of the tuckahoe coarse particles after being boiled with water, and drying at 80 ℃ to obtain clean tuckahoe coarse particles;
(2) taking 10kg of clean poria cocos coarse particles, putting the clean poria cocos coarse particles into a 40B type universal grinder for grinding, filtering the clean poria cocos coarse particles by using an 80-mesh vibrating screen to collect powder, breaking the walls by using an AB06 type jet mill, and filtering and collecting the broken walls by using a 300-mesh vibrating screen;
(3) adding 6 times of water (mass ratio of wall-broken powder to water is 1:6) into the wall-broken powder, stirring, mixing, adding β - (1 → 3) glucan hydrolase at 1.5% (w/w), temperature of 47 deg.C, pH of 4.7, stirring, and performing enzymolysis for 5 hr
(4) Heating, decocting and deactivating the enzymolysis liquid to boiling, keeping the temperature for 5 minutes, centrifuging at 4000r/min for 30 minutes, and performing suction filtration with 300 meshes to obtain pachyman filtrate.
(5) Concentrating pachyman filtrate at 75 deg.C under reduced pressure to 14% of the original volume, spray drying the concentrated solution at air inlet temperature of 170 deg.C and air outlet temperature of 75 deg.C to obtain pachyman powder, and making into pachyman solid preparation according to the requirements of preparation.
Comparative example 3
The extraction method of the pachyman extract of this comparative example was substantially the same as that of example 3, except that the wall-breaking pulverization step was omitted, specifically:
(1) taking a proper amount of the tuckahoe coarse particles after being boiled with water, and drying at 80 ℃ to obtain clean tuckahoe coarse particles;
(2) taking 10kg of clean poria cocos coarse particles, putting the clean poria cocos coarse particles into a 40B type universal pulverizer for pulverizing, and filtering by using a vibrating screen with 80 meshes to collect powder;
(3) adding 6 times of water (mass ratio of powder to water is 1:4) into the powder, stirring, mixing, grinding in JMS-80B colloid mill for 2 hr, adding β - (1 → 3) dextran hydrolase with addition amount of 1.5% (w/w), temperature of 48 deg.C, pH of 4.6, stirring, and performing enzymolysis for 5 hr
(4) Heating, decocting and deactivating the enzymolysis liquid to boiling, keeping the temperature for 5 minutes, centrifuging at 4000r/min for 30 minutes, and performing suction filtration with 300 meshes to obtain pachyman filtrate.
(5) Concentrating pachyman filtrate at 75 deg.C under reduced pressure to 16% of original volume, spray drying the concentrated solution at air inlet temperature of 170 deg.C and air outlet temperature of 76 deg.C to obtain pachyman powder, and making into pachyman solid preparation according to the requirements of preparation.
Effect testing experiment
The polysaccharide contents of the pachyman extracts of examples 1 to 3 and comparative examples 1 to 3, in which the oral liquid was subjected to a drying treatment for the purpose of testing, were measured by the phenol-sulfuric acid method, and the results of the tests are shown in the following table 1:
TABLE 1
As can be seen from table 1, the pachyman extracts of examples 1-3 all have higher polysaccharide content, which indicates that the combination of mechanical wall-breaking ultrafine grinding, mechanical grinding hydration opacification and dedicated enzymatic hydrolysis technology of the present invention effectively improves the extraction rate of pachyman.
In addition, the comparison result of the example 3 and the comparative example 1 shows that the pachyman content can be obviously improved by adopting the exclusive β - (1 → 3) glucan hydrolase, and the comparison result of the example 3 and the comparative examples 2 and 3 shows that the reduction of the pachyman content can be caused by omitting the steps of mechanical wall-breaking superfine grinding and mechanical grinding hydration opacification.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The pachyman extraction method is characterized by comprising the following steps:
providing a crude tuckahoe product;
micronizing the crude product of Poria to obtain Poria wall-broken powder;
mixing the poria cocos wall-broken powder with water, and grinding to prepare a poria cocos emulsion;
carrying out enzymolysis on the poria cocos emulsion by adopting glucan hydrolase to prepare an enzymolysis liquid;
deactivating the enzymolysis solution, performing solid-liquid separation, and collecting filtrate to obtain pachyman extract.
2. The pachymaran extraction method of claim 1, wherein the step of providing crude pachymaran comprises the steps of:
decocting Poria with water, and collecting residue;
and drying the dregs to obtain the crude poria cocos product.
3. The pachyman extraction method according to claim 2, wherein the drying temperature of the residue is 50-80 ℃.
4. The method for extracting pachymaran according to claim 1, wherein the step of micronizing the crude pachymaran comprises the steps of:
primarily crushing the crude poria cocos product, and sieving the crude poria cocos product with a screen of 40-200 meshes to obtain a crushed poria cocos product;
and carrying out superfine grinding on the poria cocos crushed material, and sieving the crushed material by using a sieve with more than 200 meshes to obtain the poria cocos wall-broken powder.
5. The method for extracting pachymaran according to claim 4, wherein the step of mixing the wall-broken tuckahoe powder with water and grinding comprises the steps of:
mixing the poria cocos wall-broken powder and water in a mass ratio of 1: (2-8) mixing and grinding for 1-8 h.
6. The pachyman extraction process according to claim 1, characterized in that said glucanohydrolases are β - (1 → 3) glucanohydrolases.
7. The method for extracting pachymaran according to claim 6, wherein the concentration of the β - (1 → 3) glucan hydrolase in the enzymolysis solution is 1-3%, the enzymolysis temperature is 40-60 ℃, the enzymolysis time is 4-8 h, and the pH value of the enzymolysis solution is 3-6.
8. The pachyman extraction process according to any one of claims 1 to 7, characterized in that it further comprises, after the step of collecting the filtrate, the steps of:
concentrating the filtrate at the temperature of 50-85 ℃ to 10-20% of the volume of the filtrate to obtain a concentrated solution;
and drying the concentrated solution by adopting a spray drying method, wherein the drying conditions of the spray drying are that the air inlet temperature is 160-180 ℃, and the air outlet temperature is 65-85 ℃.
9. The pachyman extract obtained by the method for extracting pachyman according to any one of claims 1 to 8.
10. The combined production process of tuckahoe products is characterized by comprising the following steps:
decocting Poria with water to obtain decoction and residue;
concentrating, drying and granulating the decoction to prepare the poria cocos traditional Chinese medicine formula granules;
drying the dregs to obtain a crude tuckahoe product;
micronizing the crude product of Poria to obtain Poria wall-broken powder;
mixing the poria cocos wall-broken powder with water, and grinding to prepare a poria cocos emulsion;
carrying out enzymolysis on the poria cocos emulsion by adopting glucan hydrolase to prepare an enzymolysis liquid;
deactivating the enzymolysis solution, performing solid-liquid separation, collecting filtrate, concentrating, drying, and granulating to obtain pachyman solid granule.
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| CN113648407A (en) * | 2021-08-20 | 2021-11-16 | 广东渔跃生物技术有限公司 | Application of nocardia immunopotentiator in fish vaccine |
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| CN114377040A (en) * | 2022-01-11 | 2022-04-22 | 普洱淞茂滇草六味制药股份有限公司 | Preparation method of poria cocos instant powder |
| CN115176890A (en) * | 2022-06-29 | 2022-10-14 | 瑞普高科(天津)生物技术有限公司 | Application of poria beta-1,3-D-glucan in preparation of feed additive for improving growth performance |
| CN117815278A (en) * | 2024-03-06 | 2024-04-05 | 北京逯博士行为医学科技研究院有限公司 | Composition for assisting in reducing hypertension, hyperglycemia and hyperlipidemia and improving liver and kidney functions and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113648407A (en) * | 2021-08-20 | 2021-11-16 | 广东渔跃生物技术有限公司 | Application of nocardia immunopotentiator in fish vaccine |
| CN113813393A (en) * | 2021-09-28 | 2021-12-21 | 四川省畜牧科学研究院 | Flavoring controlled release preparation and preparation method and application thereof |
| CN114377040A (en) * | 2022-01-11 | 2022-04-22 | 普洱淞茂滇草六味制药股份有限公司 | Preparation method of poria cocos instant powder |
| CN115176890A (en) * | 2022-06-29 | 2022-10-14 | 瑞普高科(天津)生物技术有限公司 | Application of poria beta-1,3-D-glucan in preparation of feed additive for improving growth performance |
| CN117815278A (en) * | 2024-03-06 | 2024-04-05 | 北京逯博士行为医学科技研究院有限公司 | Composition for assisting in reducing hypertension, hyperglycemia and hyperlipidemia and improving liver and kidney functions and preparation method thereof |
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