CN111333025A - Cold and hot double-control temperature paper cutting chip and preparation method thereof - Google Patents
Cold and hot double-control temperature paper cutting chip and preparation method thereof Download PDFInfo
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- CN111333025A CN111333025A CN202010221809.2A CN202010221809A CN111333025A CN 111333025 A CN111333025 A CN 111333025A CN 202010221809 A CN202010221809 A CN 202010221809A CN 111333025 A CN111333025 A CN 111333025A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B81—MICROSTRUCTURAL TECHNOLOGY
- B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
- B81C3/00—Assembling of devices or systems from individually processed components
- B81C3/001—Bonding of two components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B81—MICROSTRUCTURAL TECHNOLOGY
- B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
- B81B7/00—Microstructural systems; Auxiliary parts of microstructural devices or systems
- B81B7/0083—Temperature control
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B81—MICROSTRUCTURAL TECHNOLOGY
- B81C—PROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
- B81C1/00—Manufacture or treatment of devices or systems in or on a substrate
- B81C1/00015—Manufacture or treatment of devices or systems in or on a substrate for manufacturing microsystems
- B81C1/00261—Processes for packaging MEMS devices
- B81C1/00309—Processes for packaging MEMS devices suitable for fluid transfer from the MEMS out of the package or vice versa, e.g. transfer of liquid, gas, sound
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/10—Energy storage using batteries
Abstract
The invention provides a cold and hot double-control temperature paper cutting chip and a preparation method thereof, which are characterized by comprising the following steps of S1: punching holes on the filter paper; s2: preparing materials; s3: placing a semiconductor refrigeration sheet in heat insulation cotton, attaching heat conduction silicone grease to the upper surface of an aluminum radiator, attaching a temperature sensing probe of a temperature controller to the lower surface of the aluminum radiator by using heat conduction double faced adhesive tape, and connecting a circuit according to the specification of the temperature controller; s4: arranging filter paper sheets on a flat silicon rubber sheet (or PVC glass tablecloth); s5: shearing the heat-conducting double-sided adhesive into a size suitable for adhering to the semiconductor refrigerating sheet; s6: preparing a paper-cut array chip; s7: preparing uniform forskolin phenol; s8: dripping the forinophenol on the filter paper sheet; s9: controlling the temperature to control the reaction; s10: standing the product, S11: after the experiment is finished, the heat-conducting film with the filter paper array can be peeled off, and the chip can be prepared again by replacing the heat-conducting film and the filter paper array.
Description
Technical Field
The invention relates to the field of paper chips in the field of microfluidic chips, in particular to a cold and hot double-control temperature paper cutting chip.
Background
The micro-fluidic Chip technology Lab on a Chip (LOC) is one of the most representative micro-integration technologies in the world, and the paper Chip is the latest hotspot in the field, because the paper has the advantages of low cost, environmental friendliness, easy processing and the like [1], the paper Chip has been developed greatly at present and is widely applied to the fields of clinical examination, drug analysis and the like, and the preparation technology thereof also has the existing preparation technology in the future: wax patterning techniques, inkjet printing techniques, flexographic printing techniques, paper-cut-and-folded techniques, and the like.
However, temperature control is less common in paper chip preparation and application because the portability, low cost, disposable use, etc. of paper chips are important characteristics, and if a complicated temperature control device is used, the portability, etc. of the whole system is reduced. However, the paper chip is often subjected to temperature control, which brings convenience to experiments, such as promotion of reagent volatilization at high temperature, maintenance of stability of the color generation region on the paper chip at low temperature, and the like.
Disclosure of Invention
The invention provides a cold-hot double-control-temperature paper cutting chip and a preparation method thereof, which are used for solving the technical problems in the background technology.
In order to solve the problems proposed in the background art, the technical scheme provided by the invention is as follows: the cold and hot double-control temperature paper cutting chip and the preparation method thereof are characterized by comprising the following steps:
s1: punching holes on the filter paper by adopting a standard puncher, and collecting the obtained filter paper sheets with standard sizes for later use;
s2: preparing one semiconductor refrigerating sheet, one heat insulation cotton sheet, one cooling fan with an aluminum radiator, one 12vW1209 temperature controller, 8-section 1.5V batteries, 8-section 1.5V battery boxes, heat conduction silicone grease and a roll of 50mm wide heat conduction double-sided adhesive tape;
s3: placing a semiconductor refrigeration sheet in heat insulation cotton, attaching heat conduction silicone grease to the upper surface of an aluminum radiator, attaching a temperature sensing probe of a temperature controller to the lower surface of the aluminum radiator by using heat conduction double faced adhesive tape, and connecting a circuit according to the specification of the temperature controller;
s4, arranging the collected filter paper sheets on a flat silicon rubber sheet (or PVC glass tablecloth) to form a required array such as 5 × 6;
s5: shearing the heat-conducting double-sided adhesive into a size suitable for adhering to the semiconductor refrigerating sheet;
s6: removing the coated film on the surface of the double-sided adhesive tape, pressing the double-sided adhesive tape onto the array filter paper sheet in a face-down manner, peeling off the silicon rubber sheet, and transferring the array filter paper sheet to the surface of the double-sided adhesive tape to form a paper-cut array chip;
s7: preparing 0.1mol/L of forrin phenol reagent, precisely measuring 10ul by using a liquid-transferring gun, respectively placing the reagent on each paper sheet of the paper-cut array, and switching on a circuit to control the temperature to a proper position to promote the solvent of the forrin phenol reagent to volatilize as soon as possible to obtain uniform forrin phenol;
s8: respectively taking sodium hydroxide solution of magnolol after the fulinol reagent is completely volatilized, preparing a series of standard solutions, precisely measuring 10ul by using a liquid-transferring gun, respectively adding the standard solutions on the paper sheets, respectively dropwise adding 5 filter paper sheets with each concentration, sequentially increasing the concentrations from left to right, namely 0.1121, 0.2242, 0.3363, 0.4484 and 0.5605mg/mL, then taking the filtered magnolia officinalis extract, precisely measuring 10ul by using the liquid-transferring gun, and dropwise adding the measured extract on the last row of filter paper sheets;
s9: controlling a certain temperature to accelerate the development reaction, and stopping heating after the development reaction;
s10: after standing for 10 minutes, the positive and negative electrodes of the semiconductor refrigerating sheet are reversely connected, and the temperature of the paper chip area is reduced to maintain the color development effect;
s11: after the experiment is finished, the heat-conducting film with the filter paper array can be peeled off, and the chip can be prepared again by replacing the heat-conducting film and the filter paper array.
Further, the punch diameter of the die standard punch is 6 mm.
Further, the semiconductor refrigeration piece can be selected from 1 piece or more than 1 piece to be connected in series or in parallel as required for controlling different temperature areas, such as 65-10 degrees below room temperature in 1 piece.
Further, the array of the array filter paper sheets may be 5 × 5, 5 × 6, 5 × 8, etc. distributed as needed.
Further, the size of the thermal double-sided adhesive tape is 5cm × 5cm, 5cm × 6cm, 5cm × 8cm and the like.
Furthermore, the chip is integrated on the cold and hot double-temperature control semiconductor refrigerating sheet device.
The cold-hot double-control-temperature paper cutting chip and the preparation method thereof according to claim 1 are characterized in that: the cold and hot double temperature control device comprises a semiconductor refrigeration sheet, heat insulation cotton matched with the semiconductor refrigeration sheet, one cooling fan with an aluminum radiator, one 12vW1209 temperature controller, 8-section 1.5V batteries, 8-section 1.5V battery boxes, heat conduction silicone grease and a roll of 50mm wide heat conduction double-sided adhesive tape.
Further, the paper-cut chip is prepared by arranging the filter paper sheets obtained by punching in an array according to needs and adhering the filter paper sheets to a heat-conducting double-sided adhesive tape.
Compared with the prior art, the invention has the following effects:
from the above, it can be seen that the cold-hot double-temperature-control paper cutting chip and the preparation method thereof provided by the invention are simple and easy to implement, can be conveniently carried out in a common laboratory, the paper chip is widely applied to the fields of clinical examination, analytical chemistry and the like, but the research on temperature control of the prepared paper chip is not common, because the portability and the disposability of the paper chip limit the temperature control device of the chip, if the temperature control device is too complex and the chip can not be reversibly separated, the temperature control device can not be applied to the paper chip, the method adopts the semiconductor refrigeration chip for preparation, and adopts the direct current power supply of a battery and the temperature control, and the portability of related equipment is good, so that the portability of the paper chip is not greatly influenced. Meanwhile, the device and the paper chip can be directly separated conveniently, and the throwing property of the paper chip is not influenced. But use this device control paper chip temperature can shorten relevant experiment required time greatly, can keep the colour development effect longer time after the colour development simultaneously, brought more facilities for the experiment.
Drawings
FIG. 1 is a diagram of a paper cutting chip with cold and hot control and temperature control according to an embodiment of the present invention;
FIG. 2 is a graph showing the results of detecting the total amount of magnolol in Magnolia officinalis according to the present invention.
In the figure: 1-is a standard sample zone, from left to right are 0.1121, 0.2242, 0.3363, 0.4484, 0.5605mg/mL standard magnolol solution respectively; 2-the sample area is the condition after the magnolia officinalis extract solution is developed, 3-an infrared temperature measuring gun, 4-a semiconductor refrigerating sheet, 5-a battery pack, 6-a heat radiating sheet, 7-heat conducting glue and 8-a paper shearing sheet.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments and the accompanying drawings.
It should be noted that all expressions using "first" and "second" in the embodiments of the present invention are used for distinguishing two entities with the same name but different names or different parameters, and it should be noted that "first" and "second" are merely for convenience of description and should not be construed as limitations of the embodiments of the present invention, and they are not described in any more detail in the following embodiments.
In order to solve part or all of the defects in the prior art, the invention provides a cold-hot double-control-temperature paper cutting chip and a preparation method thereof on the one hand, and the preparation method comprises the following steps:
1) punching holes on the filter paper by adopting a standard puncher, and collecting the obtained filter paper sheets with standard sizes for later use;
2) preparing one semiconductor refrigerating sheet, one heat insulation cotton sheet, one cooling fan with an aluminum radiator, one 12vW1209 temperature controller, 8-section 1.5V batteries, 8-section 1.5V battery boxes, heat conduction silicone grease and a roll of 50mm wide heat conduction double-sided adhesive tape;
3) placing a semiconductor refrigeration sheet in heat insulation cotton, attaching heat conduction silicone grease to the upper surface of an aluminum radiator, attaching a temperature sensing probe of a temperature controller to the lower surface of the aluminum radiator by using heat conduction double faced adhesive tape, and connecting a circuit according to the specification of the temperature controller;
4) the collected filter paper sheets are arranged in the desired array such as 5 × 6 on a flat sheet of silicone rubber (or PVC glass table cloth).
5) And shearing the heat-conducting double-sided adhesive into a size suitable for adhering to the semiconductor refrigerating sheet.
6) And removing the coated film on the surface of the double-sided adhesive tape, pressing the double-sided adhesive tape onto the array filter paper sheet in a face-down manner, peeling off the silicon rubber sheet, and transferring the array filter paper sheet to the surface of the double-sided adhesive tape to form the paper-cut array chip.
7) 0.1mol/L of forrin phenol reagent is prepared, 10ul of the forrin phenol reagent is precisely measured by a liquid-transferring gun and respectively placed on each paper sheet of the paper-cut array, and a circuit is connected to control the temperature to a proper position to promote the solvent of the forrin phenol reagent to be volatilized as soon as possible to obtain uniform forrin phenol.
8) After the fulinol reagent is completely volatilized, respectively taking sodium hydroxide solution of magnolol, preparing a series of standard solutions, precisely measuring 10ul by using a liquid-transferring gun, respectively adding the standard solutions on the paper sheets, respectively dropwise adding 5 filter paper sheets with each concentration, sequentially increasing the concentrations from left to right, respectively 0.1121, 0.2242, 0.3363, 0.4484 and 0.5605mg/mL, then taking the filtered magnolia officinalis extract, precisely measuring 10ul by using the liquid-transferring gun, and dropwise adding the measured extract on the last row of filter paper sheets.
9) Controlling a certain temperature to accelerate the color development reaction, and stopping heating after color development.
10) After standing for 10 minutes, the positive and negative electrodes of the semiconductor refrigerating sheet are reversely connected, and the color development effect can be maintained by reducing the area temperature of the paper chip.
11) After the experiment is finished, the heat-conducting film with the filter paper array can be peeled off, and the chip can be prepared again by replacing the heat-conducting film and the filter paper array.
In some optional embodiments, the semiconductor chilling plates can be selected from 1 or more than 1 plate to be connected in series or in parallel as required for controlling different temperature areas, such as 65 ℃ to 10 ℃ below room temperature in 1 plate.
In some alternative embodiments, the die standard punch punches are 6mm in diameter.
In some alternative embodiments, the array of filter paper sheets may be 5 × 5, 5 × 6, 5 × 8, etc. distributed as desired.
In some alternative embodiments, the bond ply size is 5cm × 5cm, 5cm × 6cm, 5cm × 8cm, and the like.
Examples
0.1mol/L sodium hydroxide is respectively prepared with a series of standard solutions of magnolol, a magnolia bark sample purchased from a traditional Chinese medicine store is taken, the powder is ground, a 1200-mesh sample sieve is used for precisely extracting a proper amount of sample, 0.1mol/L sodium hydroxide is used for ultrasonic extraction for 30min, a commercialized welfare phenol reagent is used for testing on the chip, the result is shot by a microspur mobile phone and is analyzed by ImageJ software, the standard curve result of the detection of the magnolol is shown in figure 2, the content of the determined magnolol in the magnolia bark is 2.4 percent, the result is similar to the result of the detection by an ultraviolet spectrophotometry, is the total amount of the magnolol and the magnolol, and has great advantages and strong practicability in the aspects of portability of using the chip, quality control or spot inspection in links of planting, harvesting, selling and the like of medicinal materials.
The cold and hot double-control-temperature paper cutting chip and the preparation method thereof provided by the invention are simple and easy to implement, can be conveniently carried out in a common laboratory, in the embodiment, heating can promote the reagent to be volatilized as soon as possible, and refrigeration can keep the stability of the experimental result, so that great convenience is provided for the experiment. Meanwhile, due to the fact that direct current power supply is adopted, the device does not affect portability of the paper-cut chip, and meanwhile the disposable performance of the paper-cut chip is guaranteed due to the fact that the heat-conducting double-sided adhesive tape is used. Considering that temperature control has wide requirements in the fields of PCR, immunoassay and the like, the semiconductor refrigeration direct-current cold and hot double control device provided by the method can be more widely applied in related experiments.
On the other hand, based on the preparation method in the embodiment, the invention also provides a cold and hot double-control-temperature paper-cut chip, which is prepared by adopting the cold and hot double-control-temperature paper-cut chip and the preparation method thereof.
Those of ordinary skill in the art will understand that: the discussion of any embodiment above is meant to be exemplary only, and is not intended to intimate that the scope of the disclosure, including the claims, is limited to these examples; within the idea of the invention, also features in the above embodiments or in different embodiments may be combined, steps may be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.
The embodiments of the invention are intended to embrace all such alternatives, modifications and variances that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, substitutions, improvements and the like that may be made without departing from the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (8)
1. The cold and hot double-control temperature paper cutting chip and the preparation method thereof are characterized by comprising the following steps:
s1: punching holes on the filter paper by adopting a standard puncher, and collecting the obtained filter paper sheets with standard sizes for later use;
s2: preparing one semiconductor refrigerating sheet, one heat insulation cotton sheet, one cooling fan with an aluminum radiator, one 12vW1209 temperature controller, 8-section 1.5V batteries, 8-section 1.5V battery boxes, heat conduction silicone grease and a roll of 50mm wide heat conduction double-sided adhesive tape;
s3: placing a semiconductor refrigeration sheet in heat insulation cotton, attaching heat conduction silicone grease to the upper surface of an aluminum radiator, attaching a temperature sensing probe of a temperature controller to the lower surface of the aluminum radiator by using heat conduction double faced adhesive tape, and connecting a circuit according to the specification of the temperature controller;
s4, arranging the collected filter paper sheets on a flat silicon rubber sheet (or PVC glass tablecloth) to form a required array such as 5 × 6;
s5: shearing the heat-conducting double-sided adhesive into a size suitable for adhering to the semiconductor refrigerating sheet;
s6: removing the coated film on the surface of the double-sided adhesive tape, pressing the double-sided adhesive tape onto the array filter paper sheet in a face-down manner, peeling off the silicon rubber sheet, and transferring the array filter paper sheet to the surface of the double-sided adhesive tape to form a paper-cut array chip;
s7: preparing 0.1mol/L of forrin phenol reagent, precisely measuring 10ul by using a liquid-transferring gun, respectively placing the reagent on each paper sheet of the paper-cut array, and switching on a circuit to control the temperature to a proper position to promote the solvent of the forrin phenol reagent to volatilize as soon as possible to obtain uniform forrin phenol;
s8: respectively taking sodium hydroxide solution of magnolol after the fulinol reagent is completely volatilized, preparing a series of standard solutions, precisely measuring 10ul by using a liquid-transferring gun, respectively adding the standard solutions on the paper sheets, respectively dropwise adding 5 filter paper sheets with each concentration, sequentially increasing the concentrations from left to right, namely 0.1121, 0.2242, 0.3363, 0.4484 and 0.5605mg/mL, then taking the filtered magnolia officinalis extract, precisely measuring 10ul by using the liquid-transferring gun, and dropwise adding the measured extract on the last row of filter paper sheets;
s9: controlling a certain temperature to accelerate the development reaction, and stopping heating after the development reaction;
s10: after standing for 10 minutes, the positive and negative electrodes of the semiconductor refrigerating sheet are reversely connected, and the temperature of the paper chip area is reduced to maintain the color development effect;
s11: after the experiment is finished, the heat-conducting film with the filter paper array can be peeled off, and the chip can be prepared again by replacing the heat-conducting film and the filter paper array.
2. The cold-hot double-control-temperature paper cutting chip and the preparation method thereof according to claim 1 are characterized in that: the punching diameter of the standard die puncher is 6 mm.
3. The cold-hot double-control-temperature paper cutting chip and the preparation method thereof according to claim 1 are characterized in that: the semiconductor refrigerating sheet can be selected from 1 sheet or more than 1 sheet to be connected in series or in parallel as required for controlling different temperature areas, such as 65-10 ℃ lower than room temperature in 1 sheet.
4. The chip of claim 1, wherein the array of filter paper sheets is distributed as required, such as 5 × 5, 5 × 6, 5 × 8, etc.
5. The cold-hot double-control-temperature paper cutting chip and the preparation method thereof as claimed in claim 1, wherein the size of the heat-conducting double-sided adhesive tape is 5cm × 5cm, 5cm × 6cm, 5cm × 8cm and the like.
6. The cold-hot double-control-temperature paper cutting chip and the preparation method thereof according to claim 1 are characterized in that: the chip is integrated on the cold and hot double-temperature control semiconductor refrigerating sheet device.
7. The cold-hot double-control-temperature paper cutting chip and the preparation method thereof according to claim 1 are characterized in that: the cold and hot double temperature control device comprises a semiconductor refrigeration sheet, heat insulation cotton matched with the semiconductor refrigeration sheet, one cooling fan with an aluminum radiator, one 12vW1209 temperature controller, 8-section 1.5V batteries, 8-section 1.5V battery boxes, heat conduction silicone grease and a roll of 50mm wide heat conduction double-sided adhesive tape.
8. The cold-hot double-control-temperature paper cutting chip and the preparation method thereof according to claim 1 are characterized in that: the paper-cut chip is prepared by arranging the filter paper sheets obtained by punching in an array according to needs and adhering the filter paper sheets with a heat-conducting double-sided adhesive tape.
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| JP3155842U (en) * | 2009-09-18 | 2009-12-03 | テルモ株式会社 | Component measuring device |
| CN108663419A (en) * | 2018-06-11 | 2018-10-16 | 中国科学院电子学研究所 | Paper chip and preparation method thereof and biomolecule detecting method |
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