CN215029013U - Detection chip and detection device based on paper-based microfluidic technology - Google Patents

Abstract

The utility model discloses a detect chip and detection device based on paper base micro-fluidic technology, a serial communication port, including chromatography paper, be provided with hydrophobic district and hydrophilic district on the chromatography paper, hydrophilic district sets up inside the hydrophobic district, hydrophilic district is including the sample introduction district, introduction channel, mixing area, mixing channel and the reaction color development district that communicate the setting in proper order. The quantitative agricultural product residue rapid detection device is small in size, convenient to rapidly detect, convenient to quantitatively and quantitatively, and convenient to carry, and can be used for rapidly screening agricultural product residues, and is convenient to carry, high in detection efficiency and convenient to operate.

Description

Detection chip and detection device based on paper-based microfluidic technology

Technical Field

The utility model relates to a incomplete detection technical field of farming, concretely relates to detect chip and detection device based on paper base micro-fluidic technology.

Background

In recent years, the problem of pesticide residue exceeding of agricultural products is more and more concerned by people. According to the related statistical data, the number of people who are carcinogenic due to the ingestion of vegetables, fruits or foods containing pesticide residues is increased by more than 10% each year. The current mainstream pesticide residue detection method and instrument have the problems that the field, high efficiency, rapidness and accuracy detection cannot be considered, and the requirement of rapidly screening large-batch products in the base layer including supermarkets, canteens, vegetable markets and the like is difficult to meet. In order to guarantee the food safety of the masses of people, an efficient, rapid, portable and timely agricultural product pesticide residue detection method is urgently needed to be developed.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide a detect chip and detection device based on paper base micro-fluidic technique, its is small, convenient short-term test, and the ration of being convenient for is decided regional different reagents of adding, and is with low costs, and detection efficiency is high, convenient operation, portable can satisfy basic unit and large-scale detection mechanism and carry out the demand of quick examination to the pesticide residue of agricultural product.

In order to solve the technical problem, the utility model provides a detect chip based on paper base micro-fluidic technology, including chromatography paper, be provided with hydrophobic district and hydrophilic district on the chromatography paper, hydrophilic district sets up inside the hydrophobic district, hydrophilic district is including the sample introduction district, introduction channel, mixing area, mixing channel and the reaction color development district that communicate the setting in proper order.

Preferably, the chromatography paper has a thickness of 0.15mm to 0.3 mm.

Preferably, the sample injection zone is of a circular structure, and the radius of the sample injection zone is 3.8-4.2 mm.

Preferably, the reaction color development area is of a circular structure, and the radius of the reaction color development area is 4.8-5.2 mm.

Preferably, the mixing zone is of a circular structure, and the radius of the mixing zone is 4.8-5.2 mm.

Preferably, the sample feeding channel is strip-shaped, the width of the sample feeding channel is 2.8-3.2mm, and the length of the sample feeding channel is 1-2 mm.

Preferably, the mixing channel is long-strip-shaped, the length of the mixing channel is 2-3mm, and the width of the mixing channel is equal to that of the sample feeding channel.

Preferably, the hydrophobic areas of the chromatography paper are impregnated with a hydrophobic substance.

Preferably, the hydrophobic substance is a wax.

The utility model has the advantages that:

1. the utility model discloses in, because hydrophilic district sets up in the inside of hydrophobic district, the hydrophobic district can prevent that the test solution in the hydrophilic district from flowing out hydrophilic district.

2. The utility model discloses in, reaction chromogenic region can be used to substrate and colour development agent, and the enzyme can be added to the mixing area, and the introduction area can be used to add and wait to detect the sample, under the capillary force effect of chromatography paper, waits to detect the sample and can get into the mixing area through introduction channel to the enzyme of taking the mixing area gets into reaction chromogenic region through mixing channel, later with this chip take the reading on the appearance read can.

3. The utility model provides a detection chip, it is small, convenient short-term test, and the ration of being convenient for decides regional different reagents of adding, and is with low costs, and detection efficiency is high, convenient operation, portable can satisfy basic unit and large-scale detection mechanism and carry out the demand of quick examination to the pesticide residue of agricultural product.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

The reference numbers in the figures illustrate: 10. a hydrophobic region; 20. a sample introduction area; 30. a sample introduction channel; 40. a mixing zone; 50. a mixing channel; 60. a reaction color development area.

Detailed Description

The present invention is further described with reference to the following drawings and specific embodiments so that those skilled in the art can better understand the present invention and can implement the present invention, but the embodiments are not to be construed as limiting the present invention.

Referring to fig. 1, the utility model discloses a detect chip based on paper base micro-fluidic technology, including chromatography paper, be provided with hydrophobic district 10 and hydrophilic district on the chromatography paper, hydrophilic district sets up inside hydrophobic district 10, and hydrophilic district is including the introduction of sample district 20, introduction of sample passageway 30, mixing area 40, mixing channel 50 and the reaction color development district 60 that communicate the setting in proper order. In the present invention, since the hydrophilic region is disposed inside the hydrophobic region 10, the hydrophobic region 10 can prevent the test solution in the hydrophilic region from flowing out of the hydrophilic region. For the color layer molecular paper, the color layer molecular paper is a hydrophilic material, and the hydrophobic area 10 can be formed by performing hydrophobic treatment on a partial area of the color layer analysis paper. The utility model discloses in, reaction color development district 60 can be used to substrate and colour development agent, and mixing area 40 can add the enzyme, and the introduction area can be used to add and wait to detect the sample, under the capillary force effect of chromatography paper, waits to detect the sample and can get into mixing area 40 through introduction channel 30 to the enzyme of taking mixing area 40 gets into reaction color development district 60 through mixing channel 50. And then the chip is taken to a reader for reading. The utility model provides a detection chip, it is small, convenient short-term test, and the ration of being convenient for decides regional different reagents of adding, and is with low costs, and detection efficiency is high, convenient operation, portable can satisfy basic unit and large-scale detection mechanism and carry out the demand of quick examination to the pesticide residue of agricultural product.

The thickness of the chromatography paper in the utility model is 0.15mm-0.3 mm. Specifically, the chromatography paper may have a thickness of 0.18mm, an overall chip length of 37mm and a width of 12 mm.

The sample injection area 20 is of a circular structure, and the radius of the sample injection area 20 is 3.8-4.2 mm. The reaction color development area 60 is of a circular structure, and the radius of the reaction color development area 60 is 4.8-5.2 mm. The mixing zone 40 is of circular configuration and the radius of the mixing zone 40 is 4.8-5.2 mm. Specifically, the radius of the reaction color development area 60 and the mixing area 40 can be 5mm, and the radius of the sample injection area 20 can be 4 mm.

The sample feeding channel 30 is strip-shaped, the width of the sample feeding channel 30 is 2.8-3.2mm, and the length of the sample feeding channel 30 is 1-2 mm. The mixing channel 50 is elongated, the length of the mixing channel 50 is 2-3mm, and the width of the mixing channel 50 is equal to the width of the sample feeding channel 30. Specifically, the sample introduction channel 30 and the mixing channel 50 are both rectangular strips, and the width thereof is 3 mm.

In the present invention, the hydrophobic region 10 of the chromatography paper is impregnated with a hydrophobic substance. The hydrophobic substance is wax. In the utility model, a preset pattern is printed on the chromatography paper by a wax-spraying printer, and the preset pattern is the same as the pattern of the subsequent hydrophobic area 10; thereafter, the chromatography paper printed with the wax pattern is heated by a flat heater so that the wax melts and permeates the entire chromatography paper, and then cooled down so that the wax solidifies, i.e., the above-described hydrophobic region 10 is formed. Specifically, the heating temperature of the flat heater is between 70 and 110 ℃, the time is 1 to 2min, and the method is based on that wax completely permeates chromatography paper. Certainly, for obtaining the hydrophobic pattern on the chromatography paper, the chromatography paper can also be subjected to hydrophobic treatment in other modes, for example, a stopper is used for blocking a hydrophilic region, then, a hydrophobic or super-hydrophobic film layer is prepared on the chromatography paper, and the micro-nano particles with hydrophobic groups in the film layer enter fiber gaps of the chromatography paper, so that the chromatography paper in the region has hydrophobic performance.

To the utility model discloses in the detection chip who obtains, its detection step is as follows: (1) firstly, manually dripping 3.5 microliters of substrate in a reaction color development area 60; (2) after waiting for one minute, 3.5 microliters of color developing agent is then manually dropped into the reaction color development zone 60; (3) then, manually dripping 3-4.5 microliters of enzyme into the mixing area 40, wherein the specific dosage is that liquid just fills the circular area of the mixing area 40 and does not immerse into other areas; (4) waiting for 8-10 minutes until the liquid in the reaction color development area 60 and the liquid in the mixing area 40 are completely dried; (5) then, dripping the sample for 3 times in the sample introduction region 20, wherein each time is 10 microliters, the dripping is carried out for the first time until the liquid is filled in the mixing region 40 and is not immersed in the reaction chromogenic region 60, and the subsequent dripping is carried out for two times after waiting for 1 minute so that the liquid is immersed in the reaction chromogenic region 60 to react with the substrate; (6) waiting for 10-15 minutes until the chip is completely dried and developed; (7) and taking the chip to a reader for reading. The sample can be detected by the detection method.

The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutes or changes made by the technical personnel in the technical field on the basis of the utility model are all within the protection scope of the utility model. The protection scope of the present invention is subject to the claims.

Claims (10)

1. The utility model provides a detect chip based on paper base micro-fluidic technology, its characterized in that includes chromatography paper, be provided with hydrophobic district and hydrophilic district on the chromatography paper, hydrophilic district sets up inside the hydrophobic district, hydrophilic district is including the sample introduction district, introduction channel, mixing area, mixing channel and the reaction color development district that communicate the setting in proper order.

2. The paper-based microfluidic technology based detection chip of claim 1, wherein the chromatography paper has a thickness of 0.15mm to 0.3 mm.

3. The paper-based microfluidic technology based detection chip according to claim 1, wherein the sample injection area is a circular structure, and the radius of the sample injection area is 3.8-4.2 mm.

4. The paper-based microfluidic technology based detection chip of claim 1, wherein the reaction color development zone is of a circular structure, and the radius of the reaction color development zone is 4.8-5.2 mm.

5. The paper-based microfluidic technology based detection chip of claim 1, wherein the mixing region is a circular structure, and the radius of the mixing region is 4.8-5.2 mm.

6. The paper-based microfluidic technology based detection chip according to claim 1, wherein the sample injection channel is long-strip-shaped, the width of the sample injection channel is 2.8-3.2mm, and the length of the sample injection channel is 1-2 mm.

7. The paper-based microfluidic technology detection chip according to claim 6, wherein the mixing channel is elongated, the length of the mixing channel is 2-3mm, and the width of the mixing channel is equal to the width of the sample feeding channel.

8. The paper-based microfluidic technology based detection chip of claim 1, wherein the hydrophobic region of the chromatography paper is impregnated with a hydrophobic substance.

9. The paper-based microfluidic technology based detection chip of claim 8, wherein the hydrophobic substance is wax.

10. A detection device comprising a paper-based microfluidic technology-based detection chip according to any one of claims 1 to 9.

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