CN111328711A - Method for inducing pure female industrial cannabis sativa - Google Patents
Method for inducing pure female industrial cannabis sativa Download PDFInfo
- Publication number
- CN111328711A CN111328711A CN202010321504.9A CN202010321504A CN111328711A CN 111328711 A CN111328711 A CN 111328711A CN 202010321504 A CN202010321504 A CN 202010321504A CN 111328711 A CN111328711 A CN 111328711A
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- Prior art keywords
- spraying
- female
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- hemp
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/28—Cannabaceae, e.g. cannabis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
Abstract
The invention discloses a method for inducing pure female industrial hemp, which comprises the following steps: s1, preparing a spraying container for inducing the needed isopropylidene amino oxygen acetic acid and the nonvolatile acid; s2, treating the isopropylidene aminooxyacetic acid and the non-volatile acid to obtain a final product, and treating the final product to obtain a mixture A; s3, spraying the mixture A on the industrial hemp plants in the bud stage through a spraying container, and standing for reaction for 3-5 days after spraying; s4, performing secondary spraying on the plant by the mixture A, and standing for 10 days after the spraying is completed; s5, removing male buds on the hemp plants 10 days after spraying, and growing male buds on the remaining female buds, wherein the hemp plants are hermaphrodite. The method is simple and easy to operate, reduces the labor amount of workers, reduces the emasculation operation period, reduces the pollution to water resources and environment, improves the efficiency of obtaining the pure female hemp seeds, and is suitable for market popularization.
Description
Technical Field
The invention relates to the technical field of agricultural planting, in particular to a method for inducing pure female industrial hemp.
Background
Cannabis sativa (Cannabissaval.), also known as Cannabis sativa, Queen hemp, etc., ancient China called China hemp, Cannabis sativa, bitter salt, Juques, is an annual herb of Cannabis (Cannabis) of Cannabiaceae, female and male alien plant, has a long history of cultivation and utilization, has a wide range of cultivated species or wild species throughout the world, and is mainly distributed in Asia and Europe. The hemp is a treasure, has great economic value, and relates to various aspects such as textile, paper making, food, building materials, pharmacy and the like. However, the cannabis plant contains Tetrahydrocannabinol (THC), an active ingredient causing hallucinogenic addiction, and is classified as a drug original plant in the drug contraband convention of the united nations. In order to benefit and avoid harm, the hemp variety with THC content less than 0.3% in hemp plant is internationally called industrial hemp, the THC content in the industrial hemp is extremely low, the industrial hemp has no drug utilization value, but has extremely high economic utilization value, and the hemp can be legally planted after the planting permission is obtained. With the rapid development of industrial hemp in China, Yunnan and other places in recent years, a plurality of high-yield and high-quality industrial hemp varieties suitable for being planted in low latitude areas are currently bred and bred by the agricultural academy of sciences of Yunnan province, and the varieties are applied to a plurality of places in China and contribute to the industrial hemp industry development of China. However, hybrid varieties are rarely seen in the existing industrial hemp varieties, and the reason is that due to the characteristics of male and female hybrid strains of the industrial hemp, male plants in female parents need to be manually pulled out when the industrial hemp hybrid varieties are used for producing seeds, the manual castration operation time is long, the working efficiency is low, and the removal is not thorough enough, so that the industrial hemp is guided to pure female hemp by an induction method, but the adopted inducer contains heavy metals, so that the pollution to the environment and water resources is caused; to this end we propose a method of inducing pure female industrial cannabis.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a method for inducing pure female industrial hemp.
A method of inducing pure female industrial cannabis, comprising the steps of:
s1, preparing a spraying container for inducing the needed isopropylidene amino oxygen acetic acid and the nonvolatile acid;
s2, treating the isopropylidene aminooxyacetic acid and the non-volatile acid to obtain a final product, and treating the final product to obtain a mixture A;
s3, spraying the mixture A on the industrial hemp plants in the bud stage through a spraying container, and standing for reaction for 3-5 days after spraying;
s4, performing secondary spraying on the plant by the mixture A, and standing for 10 days after the spraying is completed;
s5, removing male buds on the hemp plants 10 days after spraying, wherein the male buds can grow from the rest female buds, and the hemp plants are hermaphrodite;
s6, obtaining primary full-female industrial hemp seeds, planting the obtained primary full-female industrial hemp seeds, and repeating the steps S4 and S5 to finally obtain the high-purity full-female industrial hemp seeds.
Further, heating an aqueous solution of the isopropylidene amino oxygen acetic acid and the nonvolatile acid, detecting that the residual quantity of the isopropylidene amino oxygen acetic acid in the raw materials is less than 0.1 percent as a reaction end point, removing water from the obtained product by a compression method, cooling and stirring the alcohol organic solvent to ensure that the solvent is filtered after the crystallization is completed, and washing and drying the solvent by the alcohol solvent to obtain the final product.
Further, the non-volatile acid is sulfuric acid, carbonic acid plus isopropylidene amino oxygen acetic acid is heated and reacted for 8 hours at the temperature of 50-100 ℃, the reaction is stopped until the residual quantity of the isopropylidene amino oxygen acetic acid in the raw materials is less than 0.1 percent, and the alcohol organic solvent is methanol.
Further, the spraying of the industrial hemp in the bud stage is carried out on the top of the main stem and the side branch of the hemp plant, a small amount of spraying liquid is carried out, and the surface of the flower bud is covered by the spraying liquid.
Further, the primary whole-female industrial hemp seed spray mixture a can obtain whole-female industrial hemp seeds without emasculation.
Furthermore, the bud period is a period when most male plants are differentiated into buds, and plants of the same male and female plants are free to pollinate through pollen scattering of the male flowers.
According to the method, the isopropylidene amino oxygen acetic acid and the non-volatile acid are used for reaction, the obtained product is free of carbonate and pollution, the use efficiency is high, the method for obtaining the female industrial hemp is simple and easy to operate, the labor amount of workers is reduced, the emasculation operation period is shortened, the pollution to water resources and the environment is reduced, the efficiency of obtaining pure female hemp seeds is improved, and the method is suitable for market popularization.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Example one
The invention provides a method for inducing pure female industrial hemp, which comprises the following steps:
s1, preparing a spraying container for inducing the needed isopropylidene amino oxygen acetic acid and the nonvolatile acid;
s2, treating the isopropylidene aminooxyacetic acid and the non-volatile acid to obtain a final product, and treating the final product to obtain a mixture A;
s3, spraying the mixture A on the industrial hemp plants in the bud stage through a spraying container, and standing for reaction for 3 days after spraying;
s4, performing secondary spraying on the plant by the mixture A, and standing for 10 days after the spraying is completed;
s5, removing male buds on the hemp plants 10 days after spraying, wherein the male buds can grow from the rest female buds, and the hemp plants are hermaphrodite;
s6, obtaining primary full-female industrial hemp seeds, planting the obtained primary full-female industrial hemp seeds, spraying the hemp with the separated stigma, and obtaining the full-female industrial hemp seeds with higher purity without castration.
Example two
The invention provides a method for inducing pure female industrial hemp, which comprises the following steps:
s1, preparing a spraying container for inducing the needed isopropylidene amino oxygen acetic acid and the nonvolatile acid;
s2, treating the isopropylidene aminooxyacetic acid and the non-volatile acid to obtain a final product, and treating the final product to obtain a mixture A;
s3, spraying the mixture A on the industrial hemp plants in the bud stage through a spraying container, and standing for reacting for 4 days after spraying;
s4, performing secondary spraying on the plant by the mixture A, and standing for 10 days after the spraying is completed;
s5, removing male buds on the hemp plants 10 days after spraying, wherein the male buds can grow from the rest female buds, and the hemp plants are hermaphrodite;
s6, obtaining primary full-female industrial hemp seeds, planting the obtained primary full-female industrial hemp seeds, spraying the hemp with the separated stigma, and obtaining the full-female industrial hemp seeds with higher purity without castration.
EXAMPLE III
The invention provides a method for inducing pure female industrial hemp, which comprises the following steps:
s1, preparing a spraying container for inducing the needed isopropylidene amino oxygen acetic acid and the nonvolatile acid;
s2, treating the isopropylidene aminooxyacetic acid and the non-volatile acid to obtain a final product, and treating the final product to obtain a mixture A;
s3, spraying the mixture A on the industrial hemp plants in the bud stage through a spraying container, and standing for reaction for 5 days after spraying;
s4, performing secondary spraying on the plant by the mixture A, and standing for 10 days after the spraying is completed;
s5, removing male buds on the hemp plants 10 days after spraying, wherein the male buds can grow from the rest female buds, and the hemp plants are hermaphrodite;
s6, obtaining primary full-female industrial hemp seeds, planting the obtained primary full-female industrial hemp seeds, spraying the hemp with the separated stigma, and obtaining the full-female industrial hemp seeds with higher purity without castration.
In the embodiment, the aqueous solution of the isopropylidene aminoxyacetic acid and the nonvolatile acid is heated, when the residual quantity of the isopropylidene aminoxyacetic acid in the raw material is detected to be less than 0.1 percent, the reaction end point is taken, the obtained product is dehydrated by a compression method, the alcohol organic solvent is cooled and stirred, the solvent is filtered after the crystallization is completed, the final product is obtained by washing and drying the alcohol solvent, the nonvolatile acid adopts sulfuric acid, carbonic acid and the isopropylidene aminoxyacetic acid are heated and reacted for 8 hours at the temperature of 50-100 ℃, the reaction is stopped until the residual quantity of the isopropylidene aminoxyacetic acid in the raw material is less than 0.1 percent, the alcohol organic solvent adopts methanol, the industrial hemp at the bud stage is sprayed to the top of the main stem and the lateral branch of the hemp plant, a small amount of spraying is carried out, the sprayed liquid covers the surface of the bud, the first full-female industrial hemp seed spraying mixture A can obtain the full-female, in the bud stage, most male plants are differentiated to form buds, and the plants of the same male and female plants are subjected to free pollination through pollen dispersal of the male flowers.
In the embodiment, firstly, the isopropylidene aminooxyacetic acid and the non-volatile acid react, the obtained product is added with water for treatment, the mixture A is sprayed on buds at the initial stage of bud emergence, secondary spraying is carried out at intervals of 3-5 days, the buds sprayed for the second time are removed after 10 days, so that male buds are separated from plants, the separated plants are normally subjected to pollen propagation, the plants are induced to be changed into individuals of hermaphrodite plants, after the primary industrial hemp seeds are obtained, the primary obtained seeds are planted again, the spraying is carried out again when the plants are differentiated into stigma, and the large batch of pure female industrial hemp seeds can be obtained without emasculation. The test data was performed to obtain the highest purity female cannabis from cannabis that was sprayed for the first time at 5 day intervals.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. A method of inducing pure industrial cannabis female comprising the steps of:
s1, preparing a spraying container for inducing the needed isopropylidene amino oxygen acetic acid and the nonvolatile acid;
s2, treating the isopropylidene aminooxyacetic acid and the non-volatile acid to obtain a final product, and treating the final product to obtain a mixture A;
s3, spraying the mixture A on the industrial hemp plants in the bud stage through a spraying container, and standing for reaction for 3-5 days after spraying;
s4, performing secondary spraying on the plant by the mixture A, and standing for 10 days after the spraying is completed;
s5, removing male buds on the hemp plants 10 days after spraying, wherein the male buds can grow from the rest female buds, and the hemp plants are hermaphrodite;
s6, obtaining primary full-female industrial hemp seeds, planting the obtained primary full-female industrial hemp seeds, spraying the hemp with the separated stigma, and obtaining the full-female industrial hemp seeds with higher purity without castration.
2. A method of inducing cannabis in the female industry as claimed in claim 1, wherein the solution of isopropylaminooxyacetic acid and non-volatile acid is heated, the end point of the reaction is determined as the residual level of isopropylaminooxyacetic acid in the starting material < 0.1%, the product obtained is subjected to a compression method to remove water, the alcoholic organic solvent is cooled and stirred to complete crystallization, the solvent is filtered, and the final product is obtained by washing and drying with the alcoholic solvent.
3. A method of inducing cannabis in the female industry as claimed in claim 2, wherein the non-volatile acid is sulphuric acid, carbonic acid plus isopropylaminooxyacetic acid is heated to 50-100 ℃ for 8 hours, the reaction is stopped until the residual amount of isopropylaminooxyacetic acid in the raw material is less than 0.1%, and the alcoholic organic solvent is methanol.
4. A method of inducing pure female industrial hemp according to claim 1, wherein said lacerated industrial hemp is sprayed in a position on top of the main stem and side branches of the hemp plant, a small amount of spraying, the spraying liquid covering the surface of the flower buds.
5. A method of inducing pure female industrial hemp according to claim 1, wherein said primary whole female industrial hemp seed spray mixture a obtains whole female industrial hemp seeds without emasculation.
6. The method of claim 1, wherein the bud stage is a stage when most male plants differentiate into buds, and the plants of the same male and female plants are free-pollinated by pollen dispersal of the male flowers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010321504.9A CN111328711A (en) | 2020-04-22 | 2020-04-22 | Method for inducing pure female industrial cannabis sativa |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010321504.9A CN111328711A (en) | 2020-04-22 | 2020-04-22 | Method for inducing pure female industrial cannabis sativa |
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| CN111328711A true CN111328711A (en) | 2020-06-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010321504.9A Pending CN111328711A (en) | 2020-04-22 | 2020-04-22 | Method for inducing pure female industrial cannabis sativa |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112715289A (en) * | 2021-01-29 | 2021-04-30 | 广西壮族自治区农业科学院 | Method for identifying and rapidly propagating female palmetto hemp plants |
| CN112841023A (en) * | 2021-01-28 | 2021-05-28 | 北大荒垦丰种业股份有限公司 | Parthenocarpic breeding method for industrial hemp and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040060528A (en) * | 2002-12-30 | 2004-07-06 | 대한민국(관리부서:농촌진흥청) | Producing method of female seed with using induction of male flower on female hemp and hemp female seed |
| US20140196164A1 (en) * | 2011-08-19 | 2014-07-10 | Kirin Holdings Kabushiki Kaisha | Method for controlling sexuality of hop |
| CN104293889A (en) * | 2013-07-16 | 2015-01-21 | 北京林业大学 | A screening method for differential expressed microRNAs of male and female organs in a dioecian plant |
| CN104335895A (en) * | 2014-09-28 | 2015-02-11 | 云南省农业科学院经济作物研究所 | Method for inducing industrial pure female marihuana |
| CN204217624U (en) * | 2014-11-22 | 2015-03-25 | 黑龙江省农业科学院大庆分院 | A kind of industrial hemp breeding pollination spacer assembly |
| CN109942454A (en) * | 2019-03-25 | 2019-06-28 | 西安集佰侬生物科技有限公司 | A kind of ethylene antagonist and preparation method thereof |
-
2020
- 2020-04-22 CN CN202010321504.9A patent/CN111328711A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040060528A (en) * | 2002-12-30 | 2004-07-06 | 대한민국(관리부서:농촌진흥청) | Producing method of female seed with using induction of male flower on female hemp and hemp female seed |
| US20140196164A1 (en) * | 2011-08-19 | 2014-07-10 | Kirin Holdings Kabushiki Kaisha | Method for controlling sexuality of hop |
| CN104293889A (en) * | 2013-07-16 | 2015-01-21 | 北京林业大学 | A screening method for differential expressed microRNAs of male and female organs in a dioecian plant |
| CN104335895A (en) * | 2014-09-28 | 2015-02-11 | 云南省农业科学院经济作物研究所 | Method for inducing industrial pure female marihuana |
| CN204217624U (en) * | 2014-11-22 | 2015-03-25 | 黑龙江省农业科学院大庆分院 | A kind of industrial hemp breeding pollination spacer assembly |
| CN109942454A (en) * | 2019-03-25 | 2019-06-28 | 西安集佰侬生物科技有限公司 | A kind of ethylene antagonist and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 夏仁学: "园艺植物性别分化的研究进展", 《植物学通报》 * |
| 房郁妍: "不同药剂对大麻性别分化的影响", 《黑龙江农业科学》 * |
| 牛庭莉等: "大麻雌化处理方法的研究进展", 《黑龙江农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112841023A (en) * | 2021-01-28 | 2021-05-28 | 北大荒垦丰种业股份有限公司 | Parthenocarpic breeding method for industrial hemp and application thereof |
| CN112715289A (en) * | 2021-01-29 | 2021-04-30 | 广西壮族自治区农业科学院 | Method for identifying and rapidly propagating female palmetto hemp plants |
| CN112715289B (en) * | 2021-01-29 | 2022-08-02 | 广西壮族自治区农业科学院 | Method for identifying and rapidly propagating female palmetto hemp plants |
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