CN111304052A - Preparation method and degradation effect of atrazine degradation microbial inoculum - Google Patents
Preparation method and degradation effect of atrazine degradation microbial inoculum Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 230000000694 effects Effects 0.000 title claims abstract description 20
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 15
- 230000015556 catabolic process Effects 0.000 title claims description 17
- 238000006731 degradation reaction Methods 0.000 title claims description 17
- 230000001580 bacterial effect Effects 0.000 claims abstract description 68
- 239000000843 powder Substances 0.000 claims abstract description 44
- 238000005485 electric heating Methods 0.000 claims abstract description 30
- 238000001035 drying Methods 0.000 claims abstract description 29
- 230000000593 degrading effect Effects 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- 238000005070 sampling Methods 0.000 claims abstract description 16
- 238000000926 separation method Methods 0.000 claims abstract description 9
- 239000011521 glass Substances 0.000 claims abstract description 5
- 238000000870 ultraviolet spectroscopy Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 18
- 235000015097 nutrients Nutrition 0.000 claims description 18
- 239000000725 suspension Substances 0.000 claims description 16
- 238000007710 freezing Methods 0.000 claims description 15
- 230000008014 freezing Effects 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000004321 preservation Methods 0.000 claims description 14
- 238000004108 freeze drying Methods 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000007689 inspection Methods 0.000 claims description 10
- 230000010355 oscillation Effects 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 8
- 239000008223 sterile water Substances 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- 241001052560 Thallis Species 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 230000001502 supplementing effect Effects 0.000 claims description 6
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 239000012880 LB liquid culture medium Substances 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000003223 protective agent Substances 0.000 claims description 3
- 239000010802 sludge Substances 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 239000010410 layer Substances 0.000 claims 7
- 239000011241 protective layer Substances 0.000 claims 2
- 230000000813 microbial effect Effects 0.000 abstract description 9
- 238000000034 method Methods 0.000 description 8
- 238000007599 discharging Methods 0.000 description 6
- 238000009413 insulation Methods 0.000 description 5
- 238000007605 air drying Methods 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000233866 Fungi Species 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000004009 herbicide Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001694 spray drying Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- JYEUMXHLPRZUAT-UHFFFAOYSA-N 1,2,3-triazine Chemical compound C1=CN=NN=C1 JYEUMXHLPRZUAT-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- 230000001766 physiological effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
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- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention belongs to the technical field of bacterial strains, and particularly relates to a preparation method of an atrazine degrading microbial inoculum and a degrading effect thereof, which comprise a culture module, a separation module, a powder preparation module, a drying module and a sampling module, wherein the culture module comprises an electric heating constant temperature incubator, the separation module protects a high-speed centrifuge and a separator, the powder preparation module comprises a freeze dryer, the drying module comprises an electric heating constant temperature blast drying device, the sampling module comprises an ultraviolet visible spectrophotometer, the electric heating constant temperature incubator comprises an outer box I, the outer front side of the outer box I is provided with a switch I, the front side of the switch I is provided with observation glass, the bottom end of the outer box I is provided with four supporting legs, the bottom end of the supporting legs is provided with universal wheels, the top end of the outer box I is provided with a control module I, and the microbial inoculum of the atrazine degrading microbial inoculum with high activity is obtained, the microbial agent has high activity and is easy to store and transport for a long time.
Description
Technical Field
The invention belongs to the technical field of strains, and particularly relates to a preparation method and a degradation effect of an atrazine degradation microbial inoculum.
Background
Atrazine (2-chloro-4-ethylamino-6-isopropylamino-1, 3, 5-triazine) as a selective herbicide for triazine systems has become one of the most widely used herbicides 121 since the production in the 50 th 20 th century, and the results of related studies indicate that atrazine is a pollutant at environmentally relevant concentrations. Contamination by the use of atrazine is prevalent in soil and water environments. More importantly, metabolites of AT are also often detected in natural water and even in drinking water, AT concentrations even higher than the concentration of AT itself in the same area, which means that it also has a huge impact on aquatic organisms ". In addition, studies have shown attra. The preparation of the solid microbial inoculum mainly comprises two methods of spray drying and freeze drying. The spray drying method is not suitable for heat-sensitive substances, and the obtained powder has high adhesiveness and is easy to agglomerate, but has poor fluidity and high loss, and needs further treatment, and the original particle size is difficult to maintain after rehydration. The vacuum freeze drying technology is a novel drying means which is used for freezing a water-containing material at a low temperature and sublimating water into steam under the conditions of low temperature and low pressure, and dehydrating the material at the low temperature to achieve the drying purpose. Under the conditions of low temperature and vacuum, the physiological activity of the microbe is stopped, the cell activity is not easy to be damaged, and the protection effect on the thermosensitive substance is formed, and meanwhile, the ideal re-solubility is realized. Compared with the advantages of difficult pollution in microbial agent preservation, easy subpackage transportation and the like, the method has wide application in the aspect of microbial preservation, and is the most common preparation method 34 of the solid microbial agent at present. The research aims to prepare the atrazine-degrading bacteria into a microbial agent by a freeze-drying method, optimize the process of the microbial agent, explore the optimal process condition level and obtain the high-activity microbial agent of the atrazine-degrading bacteria.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a preparation method and a degradation effect of an atrazine degradation microbial inoculum, and the atrazine degradation microbial inoculum has the characteristics of high activity of a microbial inoculum and easiness in long-term storage and transportation.
In order to achieve the purpose, the invention provides the following technical scheme: the preparation method comprises a culture module, a separation module, a powder preparation module, a drying module and a sampling inspection module, wherein the culture module comprises an electric heating constant-temperature incubator, the separation module protects a high-speed centrifuge and a separator, the powder preparation module comprises a freeze dryer, the drying module comprises an electric heating constant-temperature blast drying device, the sampling inspection module comprises an ultraviolet visible spectrophotometer, the electric heating constant-temperature incubator comprises an outer box I, a first switch door is arranged on the outer front side of the outer box I, observation glass is arranged on the front side of the first switch door, four support legs are arranged at the bottom end of the outer box I, universal wheels are arranged at the bottom ends of the support legs, a first control module is arranged at the top end of the outer box I, a first alarm is electrically connected to one side of the control module I, and the first control module comprises a PLC, The device comprises a temperature sensor, a heater and a timer, wherein a display screen is arranged on the outer side of a first control module, a heat preservation layer is arranged on the inner side of a first outer box body, a plurality of placing frames are arranged in the first outer box body, a vibration generator is further arranged in the first outer box body and comprises a vibration disc, a motor, a spring group and an eccentric wheel, a nutrient solution supplementing pipe is further arranged above the placing frames, a nutrient solution discharging pipe is further arranged at the bottom of the vibration disc and the first outer box body, a discharging solution receiver is further arranged at the other end of the nutrient solution discharging pipe, a nutrient solution box is further connected with the nutrient solution supplementing pipe, the electric heating constant-temperature blast drying device comprises a second outer box body, a second switch door is arranged on the front side of the second outer box body, an observation area is arranged in the middle of the front side of the second switch door, a handle is arranged on the outer side of the second switch door, and a second control, the second control module comprises a PLC, a temperature sensor, a humidity sensor, a second alarm and a timer, a display screen of temperature and humidity is arranged on the outer side of the second control module, an air blower is installed on one side of the second outer box, and a storage rack is installed inside the second outer box.
Preferably, the shape of electric heat constant temperature incubator is the cylinder, and when the universal wheel of bottom need remove, does not need the people to carry, prevents extravagant manpower.
Preferably, the discharged liquid receiver has an upper cylindrical structure, and a waste liquid temporary storage tank is connected to a lower portion of the discharged liquid receiver through a pipe.
Preferably, the upper diameter of the discharged liquid receiver is larger than the oscillation amplitude of the oscillation plate.
Preferably, the electric heating constant-temperature air blowing drying device further comprises an air outlet and an anemometer, wherein the air outlet is located at the bottom of the outer box II, the air outlet is further connected with an air channel, and the air channel is distributed in a cavity inside the outer box II and blows air to a tray area built in the drying device in an evenly distributed mode.
Preferably, electric heat constant temperature incubator heat preservation includes inoxidizing coating, interior heat preservation, outer heat preservation, the interior inoxidizing coating of heating layer, and is the vacuum in the middle of interior heat preservation and the outer heat preservation, through being provided with the vacuum layer, gives sound insulation and thermal insulation performance is good, resources are saved.
Preferably, the electric heating constant temperature blast drying device comprises a blast blower and a heater, and the heater is arranged in the box body; air drying is carried out through an air blower, and the internal temperature is controlled through a temperature sensor to keep constant.
Preferably, the electric heating constant temperature incubator and the electric heating constant temperature blast drying device are both provided with alarms, and the alarms are electrically connected with the control module; when abnormal phenomenon occurs, the alarm gives an alarm and automatically cuts off the power.
Preferably, the preparation method of the atrazine degrading microbial inoculum comprises the following steps:
firstly, culturing and collecting thalli and preparing a bacterial suspension;
secondly, inoculating 1 percent of the activated bacteria into an LB liquid culture medium, setting the temperature to be 30 ℃, and performing shake culture for 180 r/min;
thirdly, placing the mixture in a constant temperature incubator at 30 ℃ for culturing for 36-48 h;
fourthly, placing the bacterial liquid into a centrifugal tube, centrifuging for 10min at 7000r/min and 4 ℃, collecting thalli, removing supernatant by using a separator, washing by using sterile water, and centrifuging again to remove the supernatant to obtain bacterial sludge;
fifthly, mixing the protective agent and the thallus according to the ratio of 1: 2, mixing to obtain the most reasonable bacterial suspension;
sixthly, subpackaging the prepared bacterial suspension into sterile 75mm culture dishes according to 12 mL;
seventhly, putting the culture dish with the bacterial suspension into a refrigerator at the temperature of 20 ℃ below zero for pre-freezing;
eighthly, after completely freezing, putting the frozen seeds into a cold trap at the temperature of-75 ℃ for continuous pre-freezing;
ninthly, after completely freezing, putting the frozen powder into a freeze dryer, and freeze-drying the frozen powder for 16 h;
tentatively, sampling inspection: sampling and weighing the freeze-dried bacterial powder, redissolving the bacterial powder into sterile water completely equal to the bacterial source, shaking the bacterial powder to fully dissolve the bacterial powder, dissolving the bacterial powder in a water bath at 30 ℃ for 30min, carrying out lamp freeze-drying on the bacterial powder by adopting a flat plate dilution coating method, counting effective viable bacteria, placing the bacterial powder in a constant-temperature incubator at 30 ℃, and checking whether the bacterial strain is qualified.
Compared with the prior art, the invention has the beneficial effects that: the microbial agent of the atrazine degrading bacteria with high activity is obtained, the activity of the microbial agent is high, and the long-term storage and transportation are easy.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the flow structure of the present invention;
FIG. 2 is a schematic view of the outer side structure of the electric heating thermostatic incubator of the present invention;
FIG. 3 is a schematic view showing the internal structure of an electrothermal incubator of the present invention;
FIG. 4 is a schematic structural diagram of a front cross section of a vibration generator in the electrothermal incubator according to the present invention;
FIG. 5 is a schematic bottom view of a shock generator in the electrothermal incubator according to the present invention;
FIG. 6 is a schematic structural view of an electric heating constant temperature forced air drying device according to the present invention;
in the figure: 1. a first outer box body; 2. opening and closing the first door; 3. observing glass; 5. supporting legs; 6. a universal wheel; 7. a first control module; 8. an alarm I; 9. a display screen; 10. a heat-insulating layer; 11. a placing frame; 12. a shock generator; 1201. a vibration plate; 1202. a motor; 1203. a spring set; 1204. an eccentric wheel; 1205. a nutrient solution replenishing pipe; 1206. a nutrient solution discharge pipe; 1207. a nutrient solution tank; 13. an observation area; 14. a second outer box body; 15. opening and closing the second door; 16. a handle; 17. a second control module; 18. a blower; 19. a shelf.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1-6, the present invention provides the following technical solutions: the preparation method of the atrazine degrading microbial inoculum comprises a culture module, a separation module, a powder preparation module, a drying module and a sampling inspection module, wherein the culture module comprises an electric heating constant temperature incubator, the separation module protects a high-speed centrifuge and a separator, the powder preparation module comprises a freeze dryer, the drying module comprises an electric heating constant temperature blast drying device, the sampling inspection module comprises an ultraviolet visible spectrophotometer, the electric heating constant temperature incubator comprises an outer box body I1, a switch door I2 is arranged on the outer front side of the outer box body I1, an observation glass 3 is arranged on the front side of the switch door I2, four support legs 5 are arranged at the bottom end of the outer box body I1, universal wheels 6 are arranged at the bottom ends of the support legs 5, a control module I7 is arranged at the top end of the outer box body I1, and an alarm I8 is electrically connected to one side of the control module I7, the control module I7 comprises a PLC, a temperature sensor, a heater and a timer, a display screen 9 is installed on the outer side of the control module I7, a heat insulation layer 10 is installed on the inner side of the outer box I1, a plurality of placing frames 11 are arranged inside the outer box I1, an oscillation generator 12 is further arranged inside the outer box I1, the oscillation generator 12 comprises an oscillation disc 1201, a motor 1202, a spring group 1203 and an eccentric wheel 1204, a nutrient solution supplementing pipe 1205 is further arranged above the placing frames 11, nutrient solution discharging pipes 1206 are further arranged at the bottoms of the oscillation disc 1201 and the outer box I1, a discharging liquid receiver is further arranged at the other end of each nutrient solution discharging pipe 1206, the nutrient solution supplementing pipe 1205 is further connected with a nutrient solution box 1207, the electric heating constant-temperature air blowing drying device comprises an outer box II 14, a switch door II 15 is installed on the front side of the outer box II 14, observation area 13 has been seted up at switch two 15 front sides middle parts, handle 16 has been installed in the outside of switch two 15, control module two 17 has been installed at the top of outer box two 14, control module two 17 includes PLC, temperature sensor, humidity transducer, alarm two, time-recorder, and the outside is provided with the display screen of temperature and humidity, air-blower 18 has been installed to one side of outer box two 14, supporter 19 has been installed to the inside of outer box two 14.
Specifically, the shape of electric heat constant temperature incubator is the cylinder, and when the universal wheel 6 of bottom need remove, does not need the people to carry, prevents extravagant manpower.
Specifically, the discharged liquid receiver is of an upper cylindrical structure, and the lower part of the discharged liquid receiver is connected with a temporary waste liquid storage tank through a pipeline.
Specifically, the upper diameter of the discharge liquid receiver is larger than the oscillation amplitude of the oscillation plate.
Specifically, electric heat constant temperature blast air drying device still includes air outlet, anemograph, the air outlet is located the bottom of outer box two 14, and the air outlet still is connected with the wind channel, the wind channel distributes in the inside cavity of outer box two 14 to evenly distributed blows off wind to the built-in tray district of drying device.
Specifically, electric heat constant temperature incubator heat preservation 10 includes inoxidizing coating, interior heat preservation, outer heat preservation, the interior inoxidizing coating of heating layer, and is the vacuum in the middle of interior heat preservation and the outer heat preservation, through being provided with the vacuum layer, gives sound insulation and thermal insulation performance is good, resources are saved.
Specifically, the electric heating constant temperature blast drying device comprises a blast blower 18 and a heater, and the heater is arranged in the box body; air drying is carried out through an air blower, and the internal temperature is controlled through a temperature sensor to keep constant.
Specifically, the electric heating constant temperature incubator and the electric heating constant temperature blast drying device are both provided with alarms, and the alarms are electrically connected with the control module; when abnormal phenomenon occurs, the alarm gives out alarm and automatically cuts off power
Specifically, the preparation method of the atrazine degrading microbial inoculum comprises the following steps:
firstly, culturing and collecting thalli and preparing a bacterial suspension;
secondly, inoculating 1 percent of the activated bacteria into an LB liquid culture medium, setting the temperature to be 30 ℃, and performing shake culture for 180 r/min;
thirdly, placing the mixture in a constant temperature incubator at 30 ℃ for culturing for 36-48 h;
fourthly, placing the bacterial liquid into a centrifugal tube, centrifuging for 10min at 7000r/min and 4 ℃, collecting thalli, removing supernatant by using a separator, washing by using sterile water, and centrifuging again to remove the supernatant to obtain bacterial sludge;
fifthly, mixing the protective agent and the thallus according to the ratio of 1: 2, mixing to obtain the most reasonable bacterial suspension;
sixthly, subpackaging the prepared bacterial suspension into sterile 75mm culture dishes according to 12 mL;
seventhly, putting the culture dish with the bacterial suspension into a refrigerator at the temperature of 20 ℃ below zero for pre-freezing;
eighthly, after completely freezing, putting the frozen seeds into a cold trap at the temperature of-75 ℃ for continuous pre-freezing;
ninthly, after completely freezing, putting the frozen powder into a freeze dryer, and freeze-drying the frozen powder for 16 h;
tentatively, sampling inspection: sampling and weighing the freeze-dried bacterial powder, redissolving the bacterial powder into sterile water completely equal to the bacterial source, shaking the bacterial powder to fully dissolve the bacterial powder, dissolving the bacterial powder in a water bath at 30 ℃ for 30min, carrying out lamp freeze-drying on the bacterial powder by adopting a flat plate dilution coating method, counting effective viable bacteria, placing the bacterial powder in a constant-temperature incubator at 30 ℃, and checking whether the bacterial strain is qualified.
The working principle and the using process of the invention are as follows: prepare through the cultivation module, the separation module, the powder process module, the drying module, the spot check module, at first collect the cultivation of thallus and the preparation of bacterial suspension, 1% inoculation to LB liquid medium with the fungus after the activation, the set temperature is 30 ℃, the time is 180r/min and is carried out the shake culture, place 30 ℃ constant temperature incubator and cultivate 36-48h, place the fungus liquid in the centrifuging tube, keep centrifuging 10min under the 4 ℃ condition at 7000r/min and collect the thallus, use the separator, discard the supernatant, use behind the sterile water washing, centrifugation once more removes the supernatant, obtain bacterial mud, according to 1 with the thallus protectant and thallus: 2, the prepared bacterial suspension is most reasonable, the prepared bacterial suspension is subpackaged in a sterile 75mm culture dish according to 12mL, the culture dish in which the bacterial suspension is placed is put in a refrigerator at the temperature of minus 20 ℃ for pre-freezing, the culture dish is put in a cold trap at the temperature of minus 75 ℃ for continuous pre-freezing after being completely frozen, the culture dish is put in a freeze drier for freeze-drying within 16h of freeze-drying time, and the sampling inspection stage comprises the following steps: sampling and weighing the freeze-dried bacterial powder, redissolving the bacterial powder into sterile water completely equal to the bacterial source, shaking the bacterial powder to fully dissolve the bacterial powder, dissolving the bacterial powder in a water bath at 30 ℃ for 30min, carrying out lamp freeze-drying on the bacterial powder by adopting a flat plate dilution coating method, counting effective viable bacteria, placing the bacterial powder in a constant-temperature incubator at 30 ℃, and checking whether the bacterial strain is qualified.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The preparation method of the atrazine degrading microbial inoculum comprises a culture module, a separation module, a powder preparation module, a drying module and a sampling inspection module, wherein the culture module comprises an electric heating constant-temperature incubator, the separation module protects a high-speed centrifuge and a separator, the powder preparation module comprises a freeze dryer, the drying module comprises an electric heating constant-temperature blast drying device, and the sampling inspection module comprises an ultraviolet visible spectrophotometer and is characterized in that: the electric heating constant temperature incubator comprises a first outer box body (1), a first switch door (2) is arranged on the outer front side of the first outer box body (1), observation glass (3) is arranged on the front side of the first switch door (2), four support legs (5) are arranged at the bottom end of the first outer box body (1), universal wheels (6) are arranged at the bottom ends of the support legs (5), a first control module (7) is arranged at the top end of the first outer box body (1), one side of the first control module (7) is electrically connected with a first alarm (8), the first control module (7) comprises a PLC, a temperature sensor, a heater and a timer, a display screen (9) is arranged on the outer side of the first control module (7), a heat preservation layer (10) is arranged on the inner side of the first outer box body (1), and a plurality of placing frames (11) are arranged in the first outer box body (1), the inside of the first outer box body (1) is also provided with a vibration generator (12), the vibration generator (12) comprises a vibration disc (1201), a motor (1202), a spring set (1203) and an eccentric wheel (1204), a nutrient solution supplementing pipe (1205) is further arranged above the placing frame (11), nutrient solution discharge pipes (1206) are further arranged at the bottoms of the vibration disc (1201) and the first outer box body (1), a discharge solution receiver is further arranged at the other end of each nutrient solution discharge pipe (1206), the nutrient solution supplementing pipe (1205) is further connected with a nutrient solution box (1207), the electric heating constant-temperature air blast drying device comprises a second outer box body (14), a second switch door (15) is arranged on the front side of the second outer box body (14), an observation area (13) is arranged in the middle of the front side of the second switch door (15), and a handle (16) is arranged on the outer side of the second switch door (15), control module two (17) have been installed at the top of outer box two (14), control module two (17) include PLC, temperature sensor, humidity transducer, alarm two, time-recorder, and the outside is provided with the display screen of temperature and humidity, air-blower (18) have been installed to one side of outer box two (14), supporter (19) have been installed to the inside of outer box two (14).
2. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 1, characterized in that: the shape of the electric heating constant temperature incubator is a cylinder.
3. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 1, characterized in that: the discharged liquid receiver is of an upper cylindrical structure, and the lower part of the discharged liquid receiver is connected with a waste liquid temporary storage tank through a pipeline.
4. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 3, characterized in that: the upper diameter of the discharge liquid receiver is larger than the oscillation amplitude of the oscillation disc.
5. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 1, characterized in that: the electric heating constant-temperature air blowing drying device further comprises an air outlet and an anemometer, wherein the air outlet is located at the bottom of the second outer box body (14), the air outlet is further connected with an air channel, the air channel is distributed in a cavity inside the second outer box body (14), and the air channel is uniformly distributed to blow air to a tray area built in the drying device.
6. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 1, characterized in that: the heat-insulating layer (10) of the electric heating constant-temperature incubator comprises a protective layer, an inner heat-insulating layer, an outer heat-insulating layer and an inner protective layer in the heat-insulating layer, and the middle of the inner heat-insulating layer and the outer heat-insulating layer is vacuum.
7. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 1, characterized in that: the electric heating constant temperature blast drying device comprises a blast blower (18) and a heater, and the heater is arranged in the box body.
8. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 1, characterized in that: the electric heating constant temperature incubator and the electric heating constant temperature blast drying device are both provided with alarms, and the alarms are electrically connected with the control module.
9. The preparation method and degradation effect of the atrazine degrading bacterial agent according to claim 1, characterized in that: the preparation method of the atrazine degrading microbial inoculum comprises the following steps:
firstly, culturing and collecting thalli and preparing a bacterial suspension;
secondly, inoculating 1 percent of the activated bacteria into an LB liquid culture medium, setting the temperature to be 30 ℃, and performing shake culture for 180 r/min;
thirdly, placing the mixture in a constant temperature incubator at 30 ℃ for culturing for 36-48 h;
fourthly, placing the bacterial liquid into a centrifugal tube, centrifuging for 10min at 7000r/min and 4 ℃, collecting thalli, removing supernatant by using a separator, washing by using sterile water, and centrifuging again to remove the supernatant to obtain bacterial sludge;
fifthly, mixing the protective agent and the thallus according to the ratio of 1: 2, mixing to obtain the most reasonable bacterial suspension;
sixthly, subpackaging the prepared bacterial suspension into sterile 75mm culture dishes according to 12 mL;
seventhly, putting the culture dish with the bacterial suspension into a refrigerator at the temperature of 20 ℃ below zero for pre-freezing;
eighthly, after completely freezing, putting the frozen seeds into a cold trap at the temperature of-75 ℃ for continuous pre-freezing;
ninthly, after completely freezing, putting the frozen powder into a freeze dryer, and freeze-drying the frozen powder for 16 h;
tentatively, sampling inspection: sampling and weighing the freeze-dried bacterial powder, redissolving the bacterial powder into sterile water completely equal to the bacterial source, shaking the bacterial powder to fully dissolve the bacterial powder, dissolving the bacterial powder in a water bath at 30 ℃ for 30min, carrying out lamp freeze-drying on the bacterial powder by adopting a flat plate dilution coating method, counting effective viable bacteria, placing the bacterial powder in a constant-temperature incubator at 30 ℃, and checking whether the bacterial strain is qualified.
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