CN216192398U - Portable cell culture transfer case - Google Patents
Portable cell culture transfer case Download PDFInfo
- Publication number
- CN216192398U CN216192398U CN202122773409.5U CN202122773409U CN216192398U CN 216192398 U CN216192398 U CN 216192398U CN 202122773409 U CN202122773409 U CN 202122773409U CN 216192398 U CN216192398 U CN 216192398U
- Authority
- CN
- China
- Prior art keywords
- cell culture
- cell
- culture dish
- transfer case
- transfer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 53
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- 239000000428 dust Substances 0.000 claims abstract description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000741 silica gel Substances 0.000 claims abstract description 5
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 5
- 210000004748 cultured cell Anatomy 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 5
- 239000002121 nanofiber Substances 0.000 claims description 4
- 239000011148 porous material Substances 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims 1
- 239000012774 insulation material Substances 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 claims 1
- 229910010271 silicon carbide Inorganic materials 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 13
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 11
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 5
- 239000001569 carbon dioxide Substances 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract 1
- 230000007306 turnover Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 31
- 230000004083 survival effect Effects 0.000 description 6
- 238000005138 cryopreservation Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a portable cell culture transfer case, which comprises: the main tank body, articulated set up in the positive chamber door of main tank body, the fan, just to the dust removal sterilization net of the vertical setting in business turn over wind gap, silica gel material cell culture dish rack, the temperature control board, structural design through temperature control board and dust removal sterilization net, can guarantee that cell culture is aseptic at this in-process that shifts, air humidity, the suitable growth condition of temperature and carbon dioxide, maintain the growth state of cell in the transfer process, get rid of the unfavorable intervention factor in the transfer process, both can control the cultivation and the transfer of cell in a flexible way, can increase the utilization of resources and work efficiency between the laboratory again, the efficiency and the reliability of assurance experiment.
Description
Technical Field
The utility model relates to special equipment in the field of cell culture, which is mainly used for transferring carriers during cell culture, in particular to a portable cell culture transferring box.
Background
At present, cells are cultured by fixed cell strains among all cells, the types of the cells are limited, the cell sources are generally directly purchased from large cell banks nationwide or abroad, the cells are transported by dry ice in a frozen storage mode and sent to a required laboratory for continuous culture, the time is 3-4 days, the cell strains are recovered and amplified in the laboratory after being sent, and the cell strains are stored in liquid nitrogen tanks among the cells of the laboratory and are convenient for long-term use.
One situation common in cell culture processes is: the traditional transferring method for recovering frozen cells is characterized in that cultured cell strains are frozen and then recovered after being transferred, the process is complex and variable, a pollution source exists, cells cannot be recovered immediately after being frozen, time is delayed, the existing portable cell culture transferring box is stored in a sealed mode, the inside of a transferring box body cannot be maintained for a long time, the proper growth conditions of sterility, air humidity, temperature and carbon dioxide required by cell culture cannot be maintained, the existing portable cell culture transferring box adopts an ultraviolet lamp irradiation sterilization mode, complete sterilization and disinfection cannot be guaranteed, ultraviolet lamps have influence on the cultured cells, and variables are introduced for cell culture tests.
Disclosure of Invention
Aiming at the defects in the prior art, the utility model aims to provide a portable cell culture transfer box.
The utility model discloses an above-mentioned utility model can realize through following technical scheme:
a portable cell culture transfer case, comprising: the cell culture dish comprises a main box body, a box door, a fan, a dust removal and sterilization net, a cell culture dish placing rack and temperature control plates, wherein the air inlet and the air outlet are respectively formed in the left side and the right side of the main box body, the air inlet is provided with the fan, the box door is connected with the main box body in the front through a hinge, the dust removal and sterilization net comprises two blocks, the two blocks are respectively vertically arranged on the air inlet and the air outlet in the front, the cell culture dish placing rack is isolated in the main box body, and the temperature control plates are parallelly arranged above the cell culture dish.
By adopting the technical scheme, compared with the traditional cryopreservation resuscitation transfer method, the complex process of cell cryopreservation transfer and resuscitation is avoided, the introduction of variables in the traditional cryopreservation resuscitation transfer method is eliminated, and a pollution source in the cell culture transfer process is isolated, so that the cultured cell strains are transferred efficiently and safely.
Furthermore, an observation window made of heat-insulating materials is arranged in the middle of the box door.
By adopting the technical scheme, cell culture personnel can observe the state of the cultured cells in real time in the transfer process.
Furthermore, the dust removal sterilization net is a nano fiber filter net.
By adopting the technical scheme, the diameter of the filter pore is smaller than that of the bacteria and the fungi, the nanofiber filter screen can physically intercept the fungi and the bacteria which can cause cell culture failure, avoid the variable caused by the irradiation of the ultraviolet lamp and increase the survival rate of the cultured cells after transfer.
Further, cell culture dish rack is the flexible silica gel material.
Through adopting above-mentioned technical scheme, can steadily fix the cell culture dish on the cell culture dish rack, the silica gel material also can provide buffering anticollision effect, and the cultured cell that has avoided the burst collision to cause spills over the culture dish, has increased the stationarity that the cell culture shifted.
Further, the temperature control plate is provided with a temperature sensor.
Through adopting above-mentioned technical scheme, can adjust through temperature sensor real time monitoring, temperature in the main tank body has guaranteed when cell culture shifts the inside temperature of main tank body keeps the temperature suitable for cultivateing the cell constantly, has avoided having increased the survival rate after cultivating the cell and transferring because of the influence that temperature variation caused to cultivating the cell.
In conclusion, the utility model has the following beneficial effects:
1. through the arrangement of the air inlet, the fan and the air outlet, the portable cell culture transfer box can transfer cultured cell strains for a long time and in a long distance, and better provides suitable growth conditions of air humidity, temperature and carbon dioxide required by cell culture, thereby increasing the survival rate of transferred cells;
2. through the arrangement of the observation window, cell cultivators can observe the state of the cultured cells in real time in the transferring process;
3. by the arrangement of the dust removal and sterilization net, fungi and bacteria which can cause cell culture failure can be physically intercepted, variables caused by ultraviolet lamp irradiation are avoided, and the survival rate of cultured cells after transfer is increased;
4. by the arrangement of the cell culture dish placing frame, a cell culture dish can be stably fixed on the cell culture dish placing frame, so that the stability of cell culture transfer is improved;
5. through the setting of control by temperature change version has guaranteed when cell culture shifts the inside temperature of main tank keeps for cultivateing the suitable temperature of cell constantly, has avoided having increased the survival rate after cultivating cell transfer because of the influence that temperature variation caused to cultivating the cell.
Drawings
FIG. 1 is a front view of the planar structure of the present invention;
FIG. 2 is a left side view of the planar structure of the present invention;
fig. 3 is a schematic three-dimensional structure of the present invention.
FIG. 1 shows a main box; 2. a box door; 3. a fan; 4. a handle; 5. a dust removal sterilization net; 6. a temperature control plate; 7. placing a culture dish rack; 8. an observation window; 9. an air inlet;
10. an air outlet; 11. the lock is magnetically attracted.
Detailed Description
The utility model is described in further detail below with reference to the figures and examples.
The utility model provides a technical scheme that: a portable cell culture transfer box comprises a main box body, a box door hinged on the front side of the main box body, wherein the box door is provided with an observation window, an air inlet and an air outlet which are respectively arranged on the left side and the right side of the main box body, the air inlet is provided with a fan, two dedusting and sterilizing nets are vertically arranged on the front side of the air inlet and the air outlet respectively, the cell culture dish placing frame is isolated in the main box body, a temperature control plate is arranged above the cell culture dish in parallel, when a cell culture worker needs to transfer a cultured cell strain, the temperature control plate is only needed to be set to the temperature needed by the transferred cell strain, the fan is opened, the box door is closed, after the temperature in the main box body is kept constant at the temperature needed by the cultured cell, the box door is rapidly opened, and the prepared culture dish containing the cultured cell strain is used, place on the culture dish rack, then through magnetism inhale the lock, make firm the sealing of chamber door the main tank body, through the net that disinfects will remove dust the culture dish rack is isolated inside the main tank body, form an airtight space that only can the gas flow, for cell culture provides required aseptic, air humidity, the suitable growth condition of temperature and carbon dioxide, cell transfer in-process cell culture personnel are handheld handle on the main tank body, portable steady transfer culture cell can pass through at any time the chamber door sets up the observation window observes the real-time condition of cultivating the cell, has guaranteed long-time remote cell strain of transfer culture, has guaranteed the survival rate of cultivating the cell after the cell culture transfer.
The implementation principle of the specific embodiment is as follows: temperature control plate automatic control the internal temperature of owner box, the required sterile environment of cell is guaranteed to cultivate by the net of disinfecting that removes dust, air inlet and gas outlet reach the fan guarantees to cultivate the required air humidity of cell and the suitable growth condition of carbon dioxide among the cell transfer process, silica gel material cell culture dish rack has guaranteed the stationarity of cultivating the cell transfer.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the utility model are covered by the protection scope of the utility model.
Claims (5)
1. A portable cell culture transfer case, comprising: the cell culture dish comprises a main box body, a box door, a fan, a dust removal and sterilization net, a cell culture dish placing rack and temperature control plates, wherein the air inlet and the air outlet are respectively formed in the left side and the right side of the main box body, the air inlet is provided with the fan, the box door is connected with the main box body in a front mode through hinges, the dust removal and sterilization net comprises two blocks, the two blocks are respectively vertically arranged on the air inlet and the air outlet in a front mode, the cell culture dish placing rack is isolated in the main box body, and the temperature control plates are arranged above the cell culture dish in parallel.
2. The portable cell culture transfer case of claim 1, wherein an observation window made of heat preservation and insulation material is arranged in the middle of the case door.
3. The portable cell culture transfer case of claim 1, wherein the dust-removing sterilizing net is a nano fiber filter net, the pore diameter of the nano fiber filter net is smaller than the diameter of bacteria and fungi, and the bacteria and fungi which cause the pollution of cultured cells can be completely blocked.
4. The portable cell culture transfer case of claim 1, wherein the cell culture dish rack is located between the dust-removing sterilization nets and is made of deformable silica gel.
5. The portable cell culture transfer case of claim 1, wherein the thermal control plate is a silicon carbide heating plate with a temperature sensor, and the temperature in the case can be automatically adjusted by the thermal control plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122773409.5U CN216192398U (en) | 2021-11-13 | 2021-11-13 | Portable cell culture transfer case |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122773409.5U CN216192398U (en) | 2021-11-13 | 2021-11-13 | Portable cell culture transfer case |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216192398U true CN216192398U (en) | 2022-04-05 |
Family
ID=80910701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122773409.5U Expired - Fee Related CN216192398U (en) | 2021-11-13 | 2021-11-13 | Portable cell culture transfer case |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216192398U (en) |
-
2021
- 2021-11-13 CN CN202122773409.5U patent/CN216192398U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220405 |
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| CF01 | Termination of patent right due to non-payment of annual fee |