CN111298696A - Micro-channel structure for introducing multiple sheath liquid flows and micro-fluidic chip thereof - Google Patents
Micro-channel structure for introducing multiple sheath liquid flows and micro-fluidic chip thereof Download PDFInfo
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- CN111298696A CN111298696A CN202010161822.3A CN202010161822A CN111298696A CN 111298696 A CN111298696 A CN 111298696A CN 202010161822 A CN202010161822 A CN 202010161822A CN 111298696 A CN111298696 A CN 111298696A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
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Abstract
The invention relates to the technical field of microfluidics, in particular to a micro-channel structure for introducing a plurality of sheath liquid flows and a microfluidic chip thereof. The invention can reduce the time required by the particles of the sample solution to reach the focusing position, reduce the influence on the physiological activity of the particles and simultaneously realize the purpose of rapid particle focusing.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a micro-channel structure for introducing a plurality of sheath liquid flows and a microfluidic chip thereof.
Background
The micro-fluidic technology is a technology for carrying out operations such as heat transfer, mass transfer, detection and the like on trace fluid by means of applying pressure to the fluid in a micro-scale flow channel, and relates to numerous subject fields such as microelectronics, biology, chemistry and the like. The phenomena of inertial focusing effect, vortex, laminar flow and the like generally exist in fluid flowing in the micro-scale flow channel. The use of the above-mentioned fluidic phenomena in microfluidic technology has led to extensive research, particularly in inertial focusing technology, which is associated with particle manipulation, and is a significant fraction of microfluidic technology that is not negligible.
Several microfluidic devices for single flow focusing of cells and particles have emerged over the years. Inertial microfluidic technology can achieve efficient passive focusing of cells and particles. However, the presence of multiple equilibrium locations in a rectangular microchannel requires a complex solution involving manipulation of the microchannel structure to achieve a single flow, but often the channel structure required to achieve particle focusing is too long. The existing focusing flow channel is formed by alternately connecting a contraction straight flow channel and an expansion triangular flow channel, but the flow channel structure is too long, so that the flow time is too long, and the particles are easily damaged.
Disclosure of Invention
The invention aims to overcome the defect of overlong flowing time of the existing focusing flow channel, and provides a micro-flow channel structure for introducing a plurality of sheath liquid flows and a micro-fluidic chip thereof, which can reduce the time required by particles of a sample solution to reach a focusing position, reduce the influence on the physiological activity of the particles and simultaneously realize the purpose of rapid particle focusing.
In order to solve the technical problems, the invention adopts the technical scheme that:
the utility model provides a miniflow channel structure for introducing a plurality of sheath liquid streams, includes focusing flow channel, sheath liquid runner, focusing flow channel and sheath liquid runner are linked together through a plurality of branch flow channels, focusing flow channel includes a plurality of shrink sections and the expansion section that sets up in turn.
The invention comprises a micro-channel structure for introducing a plurality of sheath liquid flows, a focusing channel is used for allowing a sample solution with particles to flow, a sheath liquid channel is used for allowing a sheath liquid to flow, a branch channel is arranged to enable the sheath liquid in the sheath liquid channel to be introduced into an expansion section of the focusing channel, and the sample solution particles are pushed to gather at one side of the expansion section to achieve focusing effect, so that the liquid in the expansion section is disturbed in a dean vortex mode, and focusing is accelerated.
Preferably, both ends of the focusing flow channel are communicated with the outside; one end of the sheath liquid flow channel is communicated with the outside, and the other end of the sheath liquid flow channel is not communicated with the outside. The two ends of the focusing flow channel are communicated with the outside, so that liquid can flow in from one end of the focusing flow channel and flow out from the other end of the focusing flow channel; one end of the sheath fluid channel is not communicated with the outside, so that the micro-channel structure is only provided with one port for discharging fluid.
Preferably, the focusing flow channel and the sheath fluid flow channel are arranged in parallel with each other. The parallel arrangement of each other enables the liquid in the focusing flow channel and the sheath liquid flow channel to be led in parallel, and particle focusing is realized through the disturbance acceleration of a plurality of dean flows.
Preferably, an included angle of 30-90 degrees is formed between the focusing flow channel and the branch flow channel. The arrangement of the included angle between the focusing flow channel and the branch flow channel can ensure that the liquid in the focusing flow channel and the liquid in the sheath liquid flow channel can be smoothly mixed and output.
Preferably, the focusing channel and the sheath fluid channel are both annular channels with notches, and the focusing channel and the sheath fluid channel are concentrically and annularly arranged. The arrangement of the annular flow channel can facilitate the introduction of centrifugal force into the micro-flow channel structure, and shorten the time required for particles to reach a focusing position.
Preferably, an included angle of 30-90 degrees is formed between the liquid flowing direction of the branch flow channel and the tangential direction of the intersection point of the branch flow channel and the focusing flow channel. The included angle between the focusing flow channel and the branch flow channel is set to enable the liquid in the focusing flow channel and the sheath liquid flow channel to be smoothly mixed and output.
Preferably, the length of the expansion section is 350-700 mu m, and the width of the expansion section is 350-700 mu m; the length of the contraction section is 350-1200 mu m, and the width of the contraction section is 50-200 mu m.
The invention also provides a micro-fluidic chip for introducing a plurality of sheath liquid flows, which comprises the micro-channel structure and a chip body, wherein the micro-channel structure is arranged in the chip body. The chip body can be used to protect the microchannel structure.
Preferably, the chip body comprises a substrate and a cover plate covering the substrate, and the substrate is provided with a groove matched with the micro-channel structure. The arrangement of the groove enables the micro-channel structure to be placed in the micro-fluidic chip and matched with the micro-fluidic chip, so that the micro-channel structure is protected.
Preferably, the cover plate is provided with a liquid inlet end communicated with the conveying pump and a liquid outlet end communicated with the extraction device, the liquid inlet end is communicated with the focusing flow channel and the sheath liquid flow channel, and the liquid outlet end is communicated with the focusing flow channel.
Compared with the prior art, the invention has the beneficial effects that:
(1) the focusing flow channel is used for enabling a sample solution with particles to flow, the sheath liquid flow channel is used for enabling a sheath liquid to flow, the branch flow channel enables the sheath liquid in the sheath liquid flow channel to be led into the expansion section of the focusing flow channel, the sample solution particles are pushed to be gathered on one side of the expansion section to achieve a focusing effect, disturbance is conducted in the expansion section in a dean vortex mode, and focusing is achieved in an accelerated mode.
(2) The included angle between the focusing flow channel and the branch flow channel is set to enable the liquid in the focusing flow channel and the sheath liquid flow channel to be smoothly mixed and output.
(3) The groove on the micro-fluidic chip enables the micro-channel structure to be placed in the micro-fluidic chip and matched with the micro-fluidic chip, so that the micro-channel structure is protected.
Drawings
FIG. 1 is a schematic structural view of a microchannel structure according to an embodiment 1 of the present invention for introducing a plurality of sheath fluids.
Fig. 2 is a top view of fig. 1.
Fig. 3 is a schematic structural diagram of a microfluidic chip in embodiment 1 for introducing multiple sheath fluids according to the present invention.
Fig. 4 is a plan view of a substrate of example 1 of the present invention.
Fig. 5 is a sectional view taken along the line a-a of fig. 4.
FIG. 6 is a schematic structural view of a microchannel structure according to embodiment 2 of the present invention for introducing a plurality of sheath fluids.
Fig. 7 is a top view of fig. 6.
Fig. 8 is a schematic structural diagram of a microfluidic chip in example 2 for introducing multiple sheath fluids according to the present invention.
Fig. 9 is a schematic structural view of a cover plate according to embodiment 2 of the present invention.
The graphic symbols are illustrated as follows:
1-focusing flow channel, 11-expanding section, 12-contracting section, 13-first liquid inlet, 14-first liquid outlet, 2-sheath liquid flow channel, 21-second liquid inlet, 22-closing port, 3-branching flow channel, 4-substrate, 41-groove, 5-cover plate, 51-third liquid inlet, 52-fourth liquid inlet and 53-second liquid outlet.
Detailed Description
The present invention will be further described with reference to the following embodiments. Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there is an orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is only for convenience of describing the present invention and simplifying the description, but it is not intended to indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and are not to be construed as limiting the present patent, and the specific meaning of the terms may be understood by those skilled in the art according to specific circumstances.
Example 1
Referring to fig. 1 to 5, a first embodiment of a microchannel structure for introducing multiple sheath fluids according to the present invention includes a focusing channel 1 and a sheath fluid channel 2, wherein the focusing channel 1 is connected to the sheath fluid channel 2 via a plurality of branch channels 3, and the focusing channel 1 includes a plurality of contraction sections 12 and expansion sections 11 alternately arranged in sequence.
The focusing flow channel 1 is used for enabling a sample solution with particles to flow, the sheath liquid flow channel 2 is used for enabling a sheath liquid to flow, the branch flow channel 3 enables the sheath liquid in the sheath liquid flow channel 2 to be led into the expansion section 11 of the focusing flow channel 1, the sample solution particles are pushed to be gathered on one side of the expansion section 11 to achieve a focusing effect, the liquid in the expansion section 11 is enabled to be disturbed in a dean vortex mode, and focusing is achieved in an accelerating mode.
In addition, both ends of the focusing flow channel 1 are communicated with the outside; one end of the sheath fluid flow passage 2 is communicated with the outside, and the other end is not communicated with the outside. The arrangement that both ends of the focusing flow channel 1 are communicated with the outside enables liquid to flow in from one end of the focusing flow channel 1 and flow out from the other end; one end of the sheath fluid channel 2 is not communicated with the outside, so that the micro-channel structure is only provided with one port for liquid outlet. As shown in fig. 1, in this embodiment, both ends of the focusing channel 1 are contraction sections, wherein one end is provided with a first liquid inlet 13, and the other end is provided with a first liquid outlet 14; one end of the sheath fluid channel 2 close to the first fluid inlet 13 is provided with a second fluid inlet 21, and the other end is provided with a closed port 22, namely, the end is not communicated with the outside. The number of the expansion sections 11 can be set to be 1-10, the number of the branch flow passages 3 can be set to be 1-10, and the number of the expansion sections 11 and the number of the branch flow passages 3 can be set to be consistent or inconsistent; in the present embodiment, the number of the expanding sections 11 is 5, the number of the branch flow passages 3 is 5, and the number of the expanding sections is consistent, so that the same dean vortex flow form can be disturbed in each expanding section 11.
Wherein, the focusing flow channel 1 and the sheath liquid flow channel 2 are arranged in parallel. The parallel arrangement enables the liquid in the focusing flow channel 1 and the sheath liquid flow channel 2 to be led in parallel, and particle focusing is realized through the disturbance acceleration of a plurality of dean flows. An included angle of 30-90 degrees is formed between the focusing flow channel 1 and the branch flow channel 3. The arrangement of the included angle between the focusing flow channel 1 and the branch flow channel 3 can enable the liquids in the focusing flow channel 1 and the sheath liquid flow channel 2 to be smoothly mixed and output. As shown in fig. 1, in the present embodiment, an angle between the liquid flow direction of the focusing flow channel 1 and the liquid flow direction of the branch flow channel 3 is 30 ° to 90 °.
Wherein, the length of the expanding section 11 is 350-700 μm, and the width is 350-700 μm; the length of the contraction section 12 is 350 to 1200 μm, and the width is 50 to 200 μm. As shown in fig. 2, the length direction in this embodiment refers to a direction from the first inlet port 13 to the first outlet port 14 on the focusing flow channel 1, and the width direction refers to a direction perpendicular to the length direction; namely, the length a of the expanding section 11 is 350-700 μm, and the width d is 350-700 μm; the length b of the contraction section 12 is 350 to 1200 μm, the width c is 50 to 200 μm, and the width c of the sheath fluid channel is also 50 to 200 μm. In the present embodiment, the heights of the focusing channel 1, the sheath fluid channel 2, and the branch channel 3 are all 50 to 100 μm, and the heights of the channels can be selected according to the particle size of the solution. The radial surfaces of the focusing flow path 1, the sheath liquid flow path 2, and the branch flow path 3 may be rectangular, elliptical, or circular.
The invention also comprises a micro-fluidic chip for introducing a plurality of sheath liquid flows, which comprises the micro-channel structure and a chip body, wherein the micro-channel structure is arranged in the chip body. The chip body can be used to protect the microchannel structure.
The chip body comprises a substrate 4 and a cover plate 5 covering the substrate 4, and a groove 41 matched with the micro-channel structure is arranged on the substrate 4. The arrangement of the groove 41 enables the micro-channel structure to be placed in the micro-fluidic chip and is matched with the micro-fluidic chip, so that the micro-channel structure is protected. As shown in fig. 4, the concave portion of the groove 41 can accommodate the focusing channel 1, the sheath fluid channel 2, and the branch channel 3, i.e., can fit the micro channel structure.
Wherein, the cover plate 5 is provided with a liquid inlet end communicated with the delivery pump and a liquid outlet end communicated with the extraction device, the liquid inlet end is communicated with the focusing flow channel 1 and the sheath liquid flow channel 2, and the liquid outlet end is communicated with the focusing flow channel 1. As shown in fig. 3 to fig. 5, in the present embodiment, the cover plate 5 is provided with a third liquid inlet 51, a fourth liquid inlet 52 and a second liquid outlet 53, the third liquid inlet 51 is communicated with the first liquid inlet 13, the fourth liquid inlet 52 is communicated with the second liquid inlet 21, and the second liquid outlet 53 is communicated with the first liquid outlet 14.
When the micro-channel structure is used, the micro-channel structure is placed in the groove 41, then the third liquid inlet 51 is communicated with a conveying pump for conveying a sample solution with particles, the fourth liquid inlet 52 is communicated with the conveying pump for conveying sheath liquid, and the second liquid outlet 53 is communicated with an extraction device; injecting a sample solution into the focusing flow channel 1, wherein the sample solution passes through a plurality of contraction sections 12 and expansion sections 11, and particles with different particle sizes in the sample solution are respectively arranged into a straight line due to the action of inertial lifting force and dean drag force in the focusing flow channel 1; at the same time, the sheath liquid is injected into the sheath liquid flow channel 2, flows through the sheath liquid flow channel 2, flows to the focusing flow channel 1 through the plurality of branch flow channels 3, and merges into the respective expanding sections 11. To ensure the flow of the sample solution and the extraction of the mixed solution, the ratio of the flow rate of the sample solution to the flow rate of the sheath solution is about 1: 1. The flowing of the sheath liquid can accelerate the disturbance of dean vortex in each expansion section 11, so that the particles can be quickly focused, the particles with different particle sizes can be completely focused at the first liquid outlet 14, and the influence on the physiological activity of the particles caused by overlong exposure time of the shearing force applied to the particles is avoided. The shear force refers to a force caused by the rotation of the particles due to inertial lift force and dean drag force.
Example 2
The present embodiment is similar to embodiment 1, except that in the present embodiment, as shown in fig. 6 to 9, the focusing flow channel 1 and the sheath fluid flow channel 2 are both annular flow channels with notches, and the focusing flow channel 1 and the sheath fluid flow channel 2 are concentrically and annularly disposed. The arrangement of the annular flow channel can facilitate the introduction of centrifugal force into the micro-flow channel structure, and shorten the time required for particles to reach a focusing position.
Wherein, an included angle of 30-90 degrees is formed between the liquid flowing direction of the branch flow channel 3 and the tangential direction of the intersection point of the branch flow channel 3 and the focusing flow channel 1. The included angle between the focusing flow channel 1 and the branch flow channel 3 is set to enable the liquids in the focusing flow channel 1 and the sheath liquid flow channel 2 to be smoothly mixed and output. As shown in fig. 7, a straight line y is taken as the liquid flow direction of the branched flow channel 3, and a straight line x is taken as a tangent line of the intersection point of the branched flow channel 3 and the focusing flow channel 1, that is, an included angle of 30 ° to 90 ° is formed between the straight lines x and y. In this embodiment, the lines x and y are 90 ° apart.
As shown in fig. 8 and 9, the groove 41 of the microfluidic chip in this embodiment is matched with the branch channel 3, the annular focusing channel 1, and the sheath fluid channel 2, and is sufficient to accommodate the micro channel structure.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. The utility model provides a miniflow channel structure for introducing a plurality of sheath liquid flows, its characterized in that, includes focusing channel (1), sheath liquid runner (2), focusing channel (1) is linked together through a plurality of branch runner (3) with sheath liquid runner (2), focusing channel (1) includes a plurality of shrink section (12) and the expansion section (11) that set up in turn.
2. The micro flow channel structure for introducing multiple sheath fluids according to claim 1, wherein both ends of the focusing flow channel (1) communicate with the outside; one end of the sheath liquid flow passage (2) is communicated with the outside, and the other end is not communicated with the outside.
3. The micro flow channel structure for introducing multiple sheath fluid flows according to claim 2, wherein the focusing flow channel (1) and the sheath fluid flow channel (2) are arranged in parallel with each other.
4. The micro flow channel structure for introducing multiple sheath fluids according to claim 3, wherein the focusing flow channel (1) and the branch flow channel (3) have an angle of 30 ° to 90 °.
5. The micro flow channel structure for introducing multiple sheath liquid flows according to claim 2, wherein the focusing flow channel (1) and the sheath liquid flow channel (2) are both annular flow channels with notches, and the focusing flow channel (1) and the sheath liquid flow channel (2) are concentrically arranged in an annular shape.
6. The micro flow channel structure for introducing multiple sheath flows according to claim 5, wherein the liquid flow direction of the branch flow channel (3) is at an angle of 30 ° to 90 ° to the tangential direction of the intersection of the branch flow channel (3) and the focusing flow channel (1).
7. The micro flow channel structure for introducing multiple sheath fluids according to claim 1, wherein the length of the expanding section (11) is 350 to 700 μm and the width is 350 to 700 μm; the length of the contraction section (12) is 350-1200 mu m, and the width is 50-200 mu m.
8. A microfluidic chip for introducing multiple sheath fluid streams, comprising the microchannel structure of any of claims 1 to 7, further comprising a chip body, the microchannel structure being disposed within the chip body.
9. The microfluidic chip for introducing multiple sheath fluid flows according to claim 8, wherein the chip body comprises a substrate (4) and a cover plate (5) covering the substrate (4), and the substrate (4) is provided with a groove (41) matching with the micro-channel structure.
10. The microfluidic chip for introducing multiple sheath liquid flows according to claim 9, wherein the cover plate (5) is provided with a liquid inlet end for communicating with a delivery pump and a liquid outlet end for communicating with an extraction device, the liquid inlet end is communicated with the focusing flow channel (1) and the sheath liquid flow channel (2), and the liquid outlet end is communicated with the focusing flow channel (1).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010161822.3A CN111298696A (en) | 2020-03-10 | 2020-03-10 | Micro-channel structure for introducing multiple sheath liquid flows and micro-fluidic chip thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010161822.3A CN111298696A (en) | 2020-03-10 | 2020-03-10 | Micro-channel structure for introducing multiple sheath liquid flows and micro-fluidic chip thereof |
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| CN111298696A true CN111298696A (en) | 2020-06-19 |
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| CN202010161822.3A Pending CN111298696A (en) | 2020-03-10 | 2020-03-10 | Micro-channel structure for introducing multiple sheath liquid flows and micro-fluidic chip thereof |
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