CN111298034A

CN111298034A - A teabag with immunity enhancing and antiviral effects

Info

Publication number: CN111298034A
Application number: CN202010109065.5A
Authority: CN
Inventors: 叶华; 王恒斌; 孙翔; 张邦国; 罗年翠; 李全; 潘耀宗; 戴晶晶; 翟云良; 张丹丹
Original assignee: Leiyunshang Pharmaceutical Group Co ltd
Current assignee: Leiyunshang Pharmaceutical Group Co ltd
Priority date: 2020-02-21
Filing date: 2020-02-21
Publication date: 2020-06-19

Abstract

The invention discloses a tea bag with immunity improving and antiviral effects, which is prepared from 1.5-6 parts of agastache rugosus, 2.5-10 parts of honeysuckle, 1.5-6 parts of dried orange peel, 1-4 parts of reed rhizome, 1-4 parts of mulberry leaf and 1.5-6 parts of ginseng. The invention adopts the theory of treatment by syndrome differentiation to screen the raw medicinal materials of the traditional Chinese medicine, the proportion of the traditional Chinese medicine teabag provided by the invention is scientific and reasonable, and the experimental result shows that the traditional Chinese medicine teabag provided by the invention has the functions of clearing away heat and toxic material, relieving exterior syndrome and eliminating dampness, resisting virus and enhancing immunity, and can improve the capability of defending influenza virus and novel coronavirus (SARS-CoV-2 for short). The Chinese medicinal composition has the effects of clearing heat, moistening lung and the like, has a better auxiliary treatment effect on viral pneumonia and the like, is natural and safe in medicinal flavor, does not have adverse reaction after being drunk for a long time, and is suitable for people of all ages.

Description

A teabag with immunity enhancing and antiviral effects

Technical Field

The invention relates to a teabag, in particular to a teabag which is prepared from traditional Chinese medicinal materials and has the effects of improving immunity and resisting viruses.

Background

Immunity refers to the ability of the human immune system to fight infection by pathogens. With the acceleration of the pace of life, people face pressure from all aspects of families, careers, society and the like, and are often under the manifestations of low immunity such as physical weakness, malnutrition, listlessness, fatigue, weakness, appetite reduction, sleep disorder and the like. Mild immune deficiency can be naturally recovered by conditioning the body for a period of time, while severe immune deficiency not only requires intervention of medication, but also has the possibility of causing canceration.

The traditional Chinese medicine considers that the occurrence, development and prognosis of diseases depend on the elimination of vital qi and pathogenic factors and are related to the immunologic function of organisms, so that the main treatment principle of strengthening the vital qi and eliminating pathogenic factors is provided. Strengthening body resistance refers to a therapeutic principle of supporting body resistance, strengthening physique, improving body resistance to pathogens and diseases. By strengthening vital qi, the pathogenic factors are eliminated, and the health condition is recovered. The traditional Chinese medicine has an outstanding treatment effect on the hypoimmunity, and can fully mobilize the immunity of the organism according to the treatment based on syndrome differentiation of different constitutions of people.

The susceptible population of the novel coronavirus (SARS-CoV-2 for short) is the population with low immunity, and the improvement of the immunity is helpful for preventing the novel coronavirus. The symptoms of pneumonia infection caused by the novel coronavirus comprise general symptoms, severe symptoms and atypical symptoms, wherein the general symptoms mainly comprise fever, hypodynamia, dry cough and dyspnea gradually, the severe symptoms comprise blood coagulation dysfunction, metabolic acidosis which is difficult to correct, septic shock, acute respiratory distress syndrome and the like, the atypical symptoms comprise anorexia and hypodynamia, mental retardation, nausea, vomiting, diarrhea and the like which take a digestive system as initial symptoms, headache which takes nervous system symptoms as initial symptoms, conjunctivitis which takes ophthalmic symptoms as initial symptoms, and cardiovascular symptoms which take a cardiovascular symptom as initial symptoms, such as chest distress, palpitation, and mild muscular soreness of limbs or waist and back.

At present, no effective medicine for resisting novel coronavirus (SARS-CoV-2 for short) is available on the market, and the medicine has great harm to the life health of patients.

Therefore, it is necessary to research and develop a Chinese medicinal teabag with definite clinical curative effect, capable of enhancing immunity and improving antiviral ability on the basis of the prior art.

Disclosure of Invention

The purpose of the invention is as follows: the invention aims to solve the defects of the prior art and provides the traditional Chinese medicine tea bag which is scientific and reasonable in proportion, exact in curative effect and convenient to use, and has the functions of clearing away heat and toxic materials, relieving exterior syndrome and eliminating dampness, enhancing immunity, improving virus resistance and the like.

The technical scheme is as follows: in order to achieve the above purpose, the invention adopts the technical scheme that:

a teabag with immunity enhancing and antiviral effects is prepared from the following raw material medicines in parts by weight:

1.5-6 parts of wrinkled gianthyssop herb, 2.5-10 parts of honeysuckle flower, 1.5-6 parts of dried orange peel, 1-4 parts of reed rhizome, 1-4 parts of mulberry leaf and 1.5-6 parts of ginseng.

As a preferred scheme, the tea bag with the effects of improving immunity and resisting viruses is prepared from the following raw material medicines in parts by weight:

1.5-3 parts of wrinkled gianthyssop herb, 2.5-5 parts of honeysuckle flower, 1.5-3 parts of dried orange peel, 1-2 parts of reed rhizome, 1-2 parts of mulberry leaf and 1.5-3 parts of ginseng.

1.5 parts of wrinkled gianthyssop herb, 2.5 parts of honeysuckle flower, 1.5 parts of dried orange peel, 1 part of reed rhizome, 1 part of mulberry leaf and 1.5 parts of ginseng.

Functional tests show that the teabag with the effects of improving immunity and resisting viruses can improve the immunity of human bodies and has good prevention and treatment effects on influenza viruses, novel coronavirus (SARS-CoV-2 for short) and the like.

The invention screens the prescription according to the diagnosis and treatment and the clinical characteristics of pneumonia infected by new coronavirus, which belongs to the category of epidemic diseases in traditional Chinese medicine, the core pathogenesis of the disease is 'dampness, toxicity, stasis and block', the disease is located in lung and spleen, and the disease can injure collaterals and enter blood. Herba Agastaches in the teabag is used as monarch, and has effects of eliminating dampness, relieving exterior syndrome, eliminating dampness, regulating stomach function, opening striae and eliminating dampness; the dried orange peel and the honeysuckle are used as ministers to help the monarch to relieve exterior syndrome, clear heat and warm and resolve phlegm-dampness; the reed rhizome and the mulberry leaf are used as adjuvant drugs to relieve exterior syndrome, dry dampness and keep body fluid so as to keep health; ginseng, radix Ginseng, in order to relieve exterior syndrome without damaging healthy qi, dry dampness without damaging yin, strengthen middle energizer and keep healthy qi in existence and pathogenic factors in the body. The medicines are combined to play the efficacy of relieving exterior syndrome and eliminating dampness. Therefore, the tea bag formula directly attacks the core pathogenesis of pneumonia infected by new corona virus, namely 'dampness, toxicity, stasis and blockage', so that the tea bag provided by the invention has positive defense and treatment effects on new corona.

Has the advantages that: the traditional Chinese medicine teabag provided by the invention is scientific and reasonable in proportioning according to the traditional Chinese medicine theory and the theory of treatment based on syndrome differentiation, and experimental results show that the traditional Chinese medicine teabag provided by the invention has the functions of clearing away heat and toxic materials, relieving exterior syndrome, eliminating dampness, resisting viruses and enhancing immunity, and can improve the capacity of defending influenza viruses and novel coronavirus (SARS-CoV-2 for short). The Chinese medicinal composition has the effects of clearing heat, moistening lung and the like, has a good conditioning effect on viral pneumonia and the like, is natural and safe in medicinal flavor, does not have adverse reaction after being drunk for a long time, and is suitable for people of all ages.

Detailed Description

The present invention is further illustrated by the following examples, which are intended to be purely exemplary and are not intended to limit the scope of the invention, as various equivalent modifications of the invention will occur to those skilled in the art upon reading the present disclosure and fall within the scope of the appended claims.

Example 1

Example 2

3 parts of wrinkled gianthyssop herb, 5 parts of honeysuckle flower, 3 parts of dried orange peel, 2 parts of reed rhizome, 2 parts of mulberry leaf and 3 parts of ginseng.

Example 3

6 parts of wrinkled gianthyssop herb, 10 parts of honeysuckle flower, 6 parts of dried orange peel, 4 parts of reed rhizome, 4 parts of mulberry leaf and 6 parts of ginseng.

Example 4 Immunity enhancing functional test

1 materials and methods

1.1 sample preparation: taking 1.5g of wrinkled gianthyssop herb, 2.5g of honeysuckle, 1.5g of dried orange peel, 1g of reed rhizome, 1g of mulberry leaf and 1.5g of ginseng from the composition of the embodiment 1 of the invention; adding water 10 times the weight of the raw materials, decocting for 2 times (each time for 1 hr), filtering, mixing extractive solutions, concentrating to obtain extract, and storing in dry and ventilated place.

1.2 Experimental animals: SPF female mice were selected, weighing 18.4-22.5 g. The mice are randomly divided into 2 groups, wherein the first group of mice is subjected to ConA-induced splenic lymphocyte transformation and NK cell activity tests; the second group of investigational mice underwent a mouse peritoneal macrophage phagocytosis chicken red blood cell test.

1.3 dose selection and subject administration: setting 2 dose groups of 1.0ml/kg/d and 3.0 ml/kg/d; the gavage dose of the mice is 0.1ml/10 g. After the continuous gavage for one month, various indexes for enhancing the immunity are measured.

1.4 test methods: the method is carried out according to the method for testing the immunity enhancing function of the technical specification for testing and evaluating health food (2003 edition). Respectively carrying out ConA induced mouse spleen lymphocyte transformation test-MTT method; phagocytosis of chicken red blood cells by macrophages in abdominal cavity of a mouse-an in-vivo method; measurement of NK cell Activity-lactate dehydrogenase assay.

1.5 statistics of test data: performing homogeneity of variance test on each test original data by using SPSS16.0 software, performing statistical treatment on data meeting the homogeneity of variance requirement by using a pairwise comparison method of mean values between a plurality of test groups and a control group in a single-factor variance analysis method; and carrying out proper variable conversion on data with non-normal distribution or uneven variance, and carrying out statistical processing on data obtained by conversion after meeting the requirements of normal distribution or uniform variance.

1.6 judging the result: according to the technical specification of health food inspection and evaluation, the test sample has positive results in any two aspects of cellular immune function (ConA-induced splenic lymphocyte transformation of mice and DNFB-induced DTH of mice), humoral immune function (antibody-producing cell detection, phagocytosis of chicken erythrocytes by mouse abdominal cavity macrophages) and NK cell activity, and can be judged to have the function of enhancing the immune function.

2. Results of the experiment

2.1 Effect of the extracts of the invention on ConA-induced splenic lymphocyte transformation in mice

After one month of the gavage mice, a ConA-induced splenic lymphocyte transformation experiment of the mice is carried out by an MTT method, the difference value of the absorbance of the ConA-added holes and the ConA-not-added holes is calculated, the homogeneity of variance is tested to meet the requirement of the homogeneity of variance, and statistical treatment is carried out by a pairwise comparison method of mean numbers between a plurality of test groups and a control group in a single-factor variance analysis method. The specific experimental results are shown in Table 1, and the difference between the absorbance of the ConA holes in the groups of 1.0ml/kg/d and 3.0ml/kg/d and the absorbance of the ConA holes without ConA is higher than that of the ConA holes in the group of 0ml/kg/d, and the difference is statistically significant (P is less than 0.05).

TABLE 1 Effect of extracts on mouse spleen lymphocyte transformation

P < 0.05 in comparison with blank

2.2 Effect of the oral liquid of the Chinese medicinal composition of the invention on the NK cell activity of mice

One month after the gavage of the mice, the activity of NK cells of the mice is measured by a lactic acid dehydrogenase method, and the activity of the NK cells is subjected to sin^-1P^1/2(P is NK cell activity and is expressed by decimal), then, the homogeneity of variance is tested to meet the requirement of homogeneity of variance, and statistical treatment is carried out by a pairwise comparison method of mean numbers between a plurality of test groups and a control group in a single-factor variance analysis method. As can be seen from the results in Table 2, the NK cell activity of 3.0ml/kg/d group was higher than that of 0ml/kg/d group, and the difference was statistically significant (P < 0.05).

TABLE 2 Effect of extracts on NK cell Activity in mice

P < 0.05 in comparison with blank

2.3 Effect of extracts on the percentage phagocytosis and phagocytosis index of chicken erythrocytes by macrophages in mouse peritoneal cavity

One month after gastric lavage of mouse, performing phagocytosis of chicken red blood cells by macrophage in abdominal cavity of mouse by using an intra-half body method, calculating phagocytosis index and phagocytosis percentage, and sin the phagocytosis percentage^-1P^1/2(P is phagocytosis percentage, expressed by decimal) conversion, then carrying out the anophelifuge test, wherein the phagocytosis index and the phagocytosis percentage meet the anophelifuge requirement, and the anophelifuge is expressed by a single-factor formulaIn the differential analysis method, the statistical treatment is carried out by a pairwise comparison method of the mean values between a plurality of test groups and a control group. As can be seen from the results in Table 3, the percentage of phagocytosis and the phagocytosis index of chicken erythrocytes by abdominal macrophages in the 3.0ml/kg/d group of mice were statistically significant compared to the 0ml/kg/d group.

TABLE 3 Effect of extracts on phagocytosis of chicken erythrocytes by macrophages in mouse abdominal cavity and phagocytosis index

P < 0.05 in comparison with blank

According to the test result of the immunity enhancing function of the health food inspection and evaluation technical specification, the Chinese medicinal composition has the function of enhancing the immunity, can improve the function of defending against the novel coronavirus (SARS-CoV-2), reduces the morbidity or improves the recovery capability of the novel coronavirus patients.

EXAMPLE 5 evaluation of clinical efficacy

1. Data and method

1.1 study object

20 subjects with poor resistance and susceptibility to cough were collected. 10 men and 10 women, age 7-62 years.

1.2 methods of treatment

The teabag prepared in the invention in the example 1 is used for 3 bags per day. 7 days is a course of treatment, and 1-4 courses of treatment are taken continuously.

2. Clinical results

After 20 subjects take the traditional Chinese medicine tea bag provided by the invention, the physique is enhanced, and the symptoms such as cold, respiratory inflammation, pneumonia and the like are obviously relieved. The specific results are shown in table 4:

TABLE 4 curative effect of bagged tea on 20 clinical patients

The experimental results of the above table 4 show that the teabag provided by the invention has obvious adjuvant therapy effect on cold diseases or respiratory cough and other symptoms of 20 clinical patients. Is expected to improve the function of defending against the novel coronavirus (SARS-CoV-2).

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A teabag with immunity improving and antiviral effects is characterized by being prepared from the following raw material medicines in parts by weight:

2. The teabag with immunity enhancing and antiviral effects according to claim 1, which is prepared from the following raw materials in parts by weight:

3. The teabag with immunity enhancing and antiviral effects according to claim 2, which is prepared from the following raw materials in parts by weight:

4. The teabag with immunity enhancing and antiviral effects of any one of claims 1 to 3, wherein the antiviral effects are anti-influenza virus and anti-coronavirus effects.

5. The teabag with immunity enhancing and antiviral effects according to claim 4, wherein the antiviral effect is an effect against pneumonia, nephritis, gastroenteritis or neuritis caused by coronavirus.