CN111296684A - Feed for improving non-specific immunity of pelteobagrus fulvidraco - Google Patents
Feed for improving non-specific immunity of pelteobagrus fulvidraco Download PDFInfo
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- CN111296684A CN111296684A CN202010124912.5A CN202010124912A CN111296684A CN 111296684 A CN111296684 A CN 111296684A CN 202010124912 A CN202010124912 A CN 202010124912A CN 111296684 A CN111296684 A CN 111296684A
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- Prior art keywords
- parts
- feed
- pelteobagrus fulvidraco
- vitamin
- improving
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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Abstract
The invention discloses a feed for improving the nonspecific immunity of pelteobagrus fulvidraco, which comprises the following components in parts by weight: 20-25 parts of flour, 6-15 parts of rapeseed meal, 15-18 parts of soybean meal, 3-8 parts of fulvic peptide, 30-40 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1-2 parts of soybean phospholipid oil, 2-3 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.2-0.5 part of vitamin complex, 1 part of compound mineral substance, 0.1-0.3 part of choline chloride, 0.02-0.05 part of vegetable essential oil and 0.02-0.05 part of chelated zinc. The feed provided by the invention can obviously improve the non-specific immunity of the pelteobagrus fulvidraco and improve the survival capability of the pelteobagrus fulvidraco, thereby effectively improving the yield of the pelteobagrus fulvidraco.
Description
Technical Field
The invention belongs to the technical field of fish feed, and particularly relates to feed for improving non-specific immunity of pelteobagrus fulvidraco.
Background
The yellow catfish is also called yellow-hot diced and gagaria, is a small fish belonging to the genus of yellow catfish in the kneeling family of catorders, and is distributed in most natural water areas in China. The fish meat is tender, delicious in taste, rich in nutrition, less in intersomatic stings and deeply loved by consumers, and is one of rare fishes in China.
The non-specific defense mechanism of fish is generally divided into four defense lines, which are respectively protease, lysozyme and agglutinin existing in mucosal secretion; mucus cells; blood cells; cells with endocytic activity, such as endothelial cells, macrophages, granular cells and the like in organ tissues, can act during microbial invasion to remove and degrade pathogenic microorganisms and other harmful substances, however, the pelteobagrus fulvidraco has poor nonspecific immunity and is very easy to infect the pathogenic microorganisms in the culture process to cause death.
Disclosure of Invention
The invention aims to: the feed for improving the nonspecific immunity of the pelteobagrus fulvidraco is provided to improve the nonspecific immunity of the pelteobagrus fulvidraco, so that the survival rate and the yield of the pelteobagrus fulvidraco are improved.
The technical scheme adopted by the invention is as follows:
the feed for improving the nonspecific immunity of the pelteobagrus fulvidraco comprises the following components in parts by weight: 20-25 parts of flour, 6-15 parts of rapeseed meal, 15-18 parts of soybean meal, 3-8 parts of fulvic peptide, 30-40 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1-2 parts of soybean phospholipid oil, 2-3 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.2-0.5 part of vitamin complex, 1 part of compound mineral substance, 0.1-0.3 part of choline chloride, 0.02-0.05 part of vegetable essential oil and 0.02-0.05 part of chelated zinc.
Furthermore, the feed for improving the nonspecific immunity of the pelteobagrus fulvidraco comprises the following components in parts by weight: 22 parts of flour, 10 parts of rapeseed meal, 15 parts of soybean meal, 6 parts of fulvic peptide, 35 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1 part of soybean phospholipid oil, 2 parts of soybean oil, 1.5 parts of monocalcium phosphate, 0.3 part of compound vitamin, 1 part of compound mineral substance, 0.2 part of choline chloride, 0.05 part of plant essential oil and 0.025 part of chelated zinc.
Furthermore, the effective active ingredients of the plant essential oil are carvacrol and thymol, the mass fraction of the carvacrol is more than or equal to 2.5%, and the mass fraction of the thymol is more than or equal to 2.5%.
Furthermore, the compound vitamin comprises the following components in parts by weight: 0.84 part of vitamin A and 0.84 part of vitamin D30.02 part, vitamin E50 parts and vitamin K36 portions of vitamin B18 portions of vitamin B210 portions of vitamin B612 portions of vitamin B120.02 part, 50 parts of nicotinamide, 20 parts of D-pantothenic acid, 2 parts of folic acid, 0.05 part of D-biotin and 70 parts of muscular ferment.
Further, the mineral composition comprises the following components in parts by weight: 10 parts of iron, 0.8 part of copper, 2.5 parts of manganese, 12 parts of zinc, 0.12 part of iodine, 0.03 part of selenium, 0.05 part of cobalt and 25 parts of magnesium.
Further, the mass fraction of zinc in the chelated zinc is 16%.
Furthermore, the preparation method of the feed comprises the following steps:
(1) pulverizing the above raw materials twice to 90%, sieving with 80 mesh sieve, and mixing;
(2) then the mixed raw materials are modulated for 3 minutes at the temperature of 98 ℃ by a modulation demodulator;
(3) then granulating by a double-screw extrusion bulking machine at 120 ℃;
(4) and finally, drying at 80 ℃, and cooling to obtain the feed.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. zinc is an important micronutrient element, and has an important role in supporting the development of an immune system, the integrity of a non-specific immune system (skin, mucous membrane) and the like and the exertion of immune cell functions, while ordinary inorganic zinc which is used conventionally cannot be effectively absorbed by the pelteobagrus fulvidraco due to the antagonism of phytic acid which is commonly present in feed, and after the high-absorption-utilization-rate chelated zinc is added, the absorption utilization rate of the zinc element by the pelteobagrus fulvidraco is effectively improved, so that the requirement of the optimal immunity of a fish body on the zinc element is met, and the non-specific immunity capability of the pelteobagrus fulvidraco is improved;
2. according to the invention, the plant essential oil is added into the feed, the plant essential oil contains thymol and carvacrol, and the two have synergistic effects, so that the prepared feed has the effects of inhibiting the adhesion of the epizoon and killing the epizoon in the breeding period, namely, the natural resistance of the pelteobagrus fulvidraco to aeromonas hydrophila, nocardia, vibrio and streptococcus is improved, and the adhesion rate and the breeding rate of the epizoon of the pelteobagrus fulvidraco are reduced;
3. the feed component provided by the invention contains functional protein raw material-rich peptide (enzymolysis protein), which is rich in immunoregulation peptide, has the function of regulating the immunity function of fish bodies, and is matched with the chelated zinc and the plant essential oil, so that the four-defense line defense effect of the nonspecific defense mechanism of the pelteobagrus fulvidraco can be effectively improved, the nonspecific immunity of the pelteobagrus fulvidraco is fundamentally improved, and the survival capability and the yield of the pelteobagrus fulvidraco are improved.
4. The feed components and the proportion provided by the invention are designed according to the digestible energy of the pelteobagrus fulvidraco, and the protein raw materials and the energy raw materials are selected according to the digestibility of the pelteobagrus fulvidraco on different protein sources and energy sources, so that the pelteobagrus fulvidraco is ensured to have high digestibility of protein, rich peptides, mineral substances and the like in the feed, that is, the effective utilization rate of the feed is improved, and the feeding cost of the pelteobagrus fulvidraco is reduced.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Thus, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
It is noted that relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The invention provides a feed for improving the nonspecific immunity of pelteobagrus fulvidraco, which comprises the following components in parts by weight: 20-25 parts of flour, 6-15 parts of rapeseed meal, 15-18 parts of soybean meal, 3-8 parts of fulvic peptide, 30-40 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1-2 parts of soybean phospholipid oil, 2-3 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.2-0.5 part of vitamin complex, 1 part of compound mineral substance, 0.1-0.3 part of choline chloride, 0.02-0.05 part of vegetable essential oil and 0.02-0.05 part of chelated zinc.
The effective active ingredients in the plant essential oil are carvacrol and thymol, the mass fraction of the carvacrol is more than or equal to 2.5%, and the mass fraction of the thymol is more than or equal to 2.5%. The plant essential oil can be extracted by distillation or extraction, or Enyi 150 essential oil produced by Nowessox corporation can be directly purchased.
Wherein, the content composition of the vitamin complex is shown in the following table 1 (weight portions):
TABLE 1 multivitamin composition
| Name of raw materials | Number of parts |
| Vitamin A | 0.84 |
| Vitamin D3 | 0.02 |
| Vitamin E (acetate dosage form) | 50.00 |
| Vitamin K3(MNB50 dosage form) | 6.00 |
| Vitamin B1(nitrate salt) | 8.00 |
| Vitamin B2(SD dosage form) | 10.00 |
| Vitamin B6 | 12.00 |
| Vitamin B12 | 0.02 |
| Nicotinamide | 50.00 |
| D-pantothenic acid | 20.00 |
| Folic acid (SD dosage form) | 2.00 |
| D-biotin | 0.05 |
| Inositol | 70.00 |
The composition of the complex minerals is shown in table 2:
table 2 content composition of composite minerals (parts by weight):
| iron | Copper (Cu) | Manganese oxide | Zinc | Iodine | Selenium | Cobalt | Magnesium alloy |
| 10 | 0.8 | 2.5 | 12 | 0.12 | 0.03 | 0.05 | 25 |
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco comprises, by weight, 20 parts of flour, 15 parts of rapeseed meal, 18 parts of soybean meal, 8 parts of fulvic peptide, 30 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 2 parts of soybean phospholipid oil, 3 parts of soybean oil, 2 parts of monocalcium phosphate, 0.5 part of multivitamin, 1 part of compound mineral, 0.1 part of choline chloride, 0.02 part of plant essential oil and 0.05 part of chelated zinc.
The raw materials are prepared into the feed by the following preparation method:
(1) pulverizing the above raw materials twice to 90%, sieving with 80 mesh sieve, and mixing;
(2) then the mixed raw materials are modulated for 3 minutes at the temperature of 98 ℃ by a modulation demodulator;
(3) then granulating by a double-screw extrusion bulking machine at 120 ℃;
(4) and finally, drying at 80 ℃, and cooling to obtain the pelteobagrus fulvidraco feed.
Example 2
The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco comprises, by weight, 22 parts of flour, 10 parts of rapeseed meal, 15 parts of soybean meal, 6 parts of fulvic peptide, 35 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1 part of soybean phospholipid oil, 2 parts of soybean oil, 1.5 parts of monocalcium phosphate, 0.3 part of compound vitamin, 1 part of compound mineral substance, 0.2 part of choline chloride, 0.05 part of plant essential oil and 0.025 part of chelated zinc.
The raw materials are prepared into the feed by the following preparation method:
(1) pulverizing the above raw materials twice to 90%, sieving with 80 mesh sieve, and mixing;
(2) then the mixed raw materials are modulated for 3 minutes at the temperature of 98 ℃ by a modulation demodulator;
(3) then granulating by a double-screw extrusion bulking machine at 120 ℃;
(4) and finally, drying at 80 ℃, and cooling to obtain the pelteobagrus fulvidraco feed.
Example 3
The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco comprises, by weight, 25 parts of flour, 6 parts of rapeseed meal, 17 parts of soybean meal, 3 parts of fulvic peptide, 40 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1.5 parts of soybean phospholipid oil, 2.5 parts of soybean oil, 1 part of monocalcium phosphate, 0.2 part of compound vitamin, 1 part of compound mineral substance, 0.3 part of choline chloride, 0.03 part of plant essential oil and 0.02 part of chelated zinc.
The raw materials are prepared into the feed by the following preparation method:
(1) pulverizing the above raw materials twice to 90%, sieving with 80 mesh sieve, and mixing;
(2) then the mixed raw materials are modulated for 3 minutes at the temperature of 98 ℃ by a modulation demodulator;
(3) then granulating by a double-screw extrusion bulking machine at 120 ℃;
(4) and finally, drying at 80 ℃, and cooling to obtain the pelteobagrus fulvidraco feed.
Test examples
Selecting 630 tails of healthy pelteobagrus fulvidraco of 1 year, randomly dividing the tails into 7 groups, carrying out 3 repeated experiments on each group, and feeding 30 pelteobagrus fulvidraco in each repeated experiment group, wherein the feed prepared in the embodiment 1 is fed in the 1 st group, the feed prepared in the embodiment 2 is fed in the 2 nd group, the feed prepared in the embodiment 3 is fed in the 3 rd group, the feed without adding chelated zinc, plant essential oil and fulvic peptide in the embodiment 1 of the invention is fed in the 4 th group, the feed without adding chelated zinc, plant essential oil and fulvic peptide in the embodiment 1 of the invention is recorded as a comparative example 1, the feed without adding chelated zinc, plant essential oil and fulvic peptide in the embodiment 2 of the invention is fed in the 5 th group, the feed without adding chelated zinc, plant essential oil and fulvic peptide in the embodiment 3 of the invention is recorded as a comparative example 2, the feed without adding chelated zinc, plant essential.
The feed selected by the control group comprises the following components in parts by weight:
25 parts of flour, 20 parts of rapeseed meal, 25 parts of soybean meal, 32 parts of fish meal, 5 parts of corn protein powder, 5 parts of pork powder, 3 parts of soybean oil, 2 parts of monocalcium phosphate, 0.2 part of compound vitamin, 1 part of compound mineral substance and 0.2 part of choline chloride.
The test is carried out in 21 aquariums of 1.5m × 1.5m × 1m, the water temperature is kept at 24-28 ℃ and the dissolved oxygen is kept at 4-4.5mg/L during the test period, the test period is 3 months, the feed is fed for 2 times per day, and the feeding amount of each time is carried out according to the following conditions: when the weight is 1-2g, 5-6% of the feed is fed every time (namely the feeding amount is 5-6% of the weight of the yellow catfish); 2-3g of body weight, and 4-5% of the feed is fed each time; 3-5g of body weight, and 3-4% of the feed is fed each time; the weight is more than 5g, and the feeding is carried out for 2-3% each time; taking 1.2g of fish as an example, the feeding amount is 0.06-0.072g each time.
After the experiment is finished, the total survival rate of each group is calculated, the initial average initial weight of the pelteobagrus fulvidraco and the average weight after the feeding is finished are recorded, then 15 pelteobagrus fulvidraco are randomly selected from each group to extract blood, the blood of each pelteobagrus fulvidraco is divided into two parts, one part is used for determining the phagocytic function of phagocytes, and the other part is used for determining the lysozyme activity.
Table 3 shows the growth of pelteobagrus fulvidraco:
TABLE 3 statistical table of feeding results
| Group of | Example 1 | Comparative example 1 | Example 2 | Comparative example 2 | Example 3 | Comparative example 3 | Control group |
| Initial uniform weight (g/tail) | 1.2 | 1.1 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 |
| Harvest average weight (g/tail) | 51.2 | 48.7 | 53.2 | 47.9 | 51.4 | 49.0 | 47.3 |
| Survival rate (%) | 93.6 | 91.7 | 97.6 | 92.5 | 93.9 | 91.3 | 89.3 |
| Coefficient of feed | 1.09 | 1.12 | 1.01 | 1.06 | 1.04 | 1.09 | 1.18 |
Wherein:
survival rate (end of test survival fraction/initial challenge fraction) 100;
feed factor is total feed consumed/(harvest weight-initial weight).
As can be seen from table 3, after the feed provided by the invention is fed, the growth speed of the pelteobagrus fulvidraco is obviously increased, the survival rate is also increased, and the feed coefficient is reduced, so that the feed provided by the invention can effectively improve the breeding income of the pelteobagrus fulvidraco.
When the lysozyme activity is measured, standing the taken blood at room temperature for 1h, then standing at 4 ℃ for 4h, finally centrifuging at the speed of 2000r/min for 10min, and taking the upper serum as a sample for measuring the lysozyme activity; micrococcus muralis was then prepared into a suspension of 0.2mol/L with 0.067mol/L phosphate buffer solution (PBS, Ph6.4), 3ml of the suspension was placed in a centrifuge tube, and 40. mu.L of serum was added, and the initial optical density value (A) was measured at a wavelength of 540nm0) And then the liquid is subjected to water bath for 30min, the water bath temperature is 28 ℃, the liquid is placed in an ice bath for 10min after the water bath is finished, the reaction is finished, then the optical density value (A) at the moment is measured, and the activity (U) of the lysozyme is calculated. Table 4 shows the results of the measurement of the lysozyme activity of Pelteobagrus fulvidraco：
TABLE 4 measurement results of lysozyme activity
Wherein U ═ A0-A)/U。
When the phagocytic capacity of phagocytic cells is measured, blood is anticoagulated by heparin, 0.25ml of staphylococcus aureus suspension is added into the anticoagulated blood, the mixture is uniformly mixed and then is subjected to water bath for 1h, the water bath temperature is 28 ℃, 0.8ml of 0.15mol/L cold NaCl solution is added after the water bath is finished, the mixture is centrifuged at 2000r/min for 4min, supernatant is removed, then a blood cell smear is sucked by a suction tube, each blood sample is smeared with 5 pieces, the blood sample is fixed by methanol for 5-7min and then is washed by clear water, and then the blood cell smear is dyed by Giemsa for 1-5h and is washed by water and dried, and then microscopic examination. Table 5 is the results of the determination of the phagocytic ability of the phagocytic cells of pelteobagrus fulvidraco:
TABLE 5 phagocytic Capacity measurement of phagocytes
Phagocytic activity of phagocytes is expressed as a Phagocytic Percentage (PP) × 100% (number of cells participating in phagocytosis out of 100 phagocytes) and a Phagocytic Index (PI) × 100% (total number of bacteria in phagocytes/number of phagocytes participating in phagocytosis), respectively.
As can be seen from tables 4 and 5, the phagocytic ability of the phagocytic cells of the pelteobagrus fulvidraco fed with the feed of the present invention is enhanced, the activity of lysozyme is improved, and the overall nonspecific immunity of the pelteobagrus fulvidraco is effectively improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco is characterized by comprising the following components in parts by weight: 20-25 parts of flour, 6-15 parts of rapeseed meal, 15-18 parts of soybean meal, 3-8 parts of fulvic peptide, 30-40 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1-2 parts of soybean phospholipid oil, 2-3 parts of soybean oil, 1-2 parts of monocalcium phosphate, 0.2-0.5 part of vitamin complex, 1 part of compound mineral substance, 0.1-0.3 part of choline chloride, 0.02-0.05 part of vegetable essential oil and 0.02-0.05 part of chelated zinc.
2. The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco according to claim 1, which is characterized by comprising the following components in parts by weight: 22 parts of flour, 10 parts of rapeseed meal, 15 parts of soybean meal, 6 parts of fulvic peptide, 35 parts of fish meal, 3 parts of corn protein powder, 3 parts of beer yeast powder, 1 part of soybean phospholipid oil, 2 parts of soybean oil, 1.5 parts of monocalcium phosphate, 0.3 part of compound vitamin, 1 part of compound mineral substance, 0.2 part of choline chloride, 0.05 part of plant essential oil and 0.025 part of chelated zinc.
3. The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco according to claim 1, wherein the effective active ingredients of the plant essential oil are carvacrol and thymol, the mass fraction of the carvacrol is more than or equal to 2.5%, and the mass fraction of the thymol is more than or equal to 2.5%.
4. The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco according to claim 1, wherein the compound vitamins comprise the following components in parts by weight: 0.84 part of vitamin A and 0.84 part of vitamin D30.02 part, vitamin E50 parts and vitamin K36 portions of vitamin B18 portions of vitamin B210 portions of vitamin B612 portions of vitamin B120.02 part, 50 parts of nicotinamide, 20 parts of D-pantothenic acid, 2 parts of folic acid, 0.05 part of D-biotin and 70 parts of muscular ferment.
5. The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco according to claim 1, wherein the minerals comprise the following components in parts by weight: 10 parts of iron, 0.8 part of copper, 2.5 parts of manganese, 12 parts of zinc, 0.12 part of iodine, 0.03 part of selenium, 0.05 part of cobalt and 25 parts of magnesium.
6. The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco according to claim 1, wherein the mass fraction of zinc in the chelated zinc is 16%.
7. The feed for improving the nonspecific immunity of the pelteobagrus fulvidraco according to claim 1, which is characterized in that the preparation method comprises the following steps:
(1) pulverizing the above raw materials twice to 90%, sieving with 80 mesh sieve, and mixing;
(2) then the mixed raw materials are modulated for 3 minutes at the temperature of 98 ℃ by a modulation demodulator;
(3) then granulating by a double-screw extrusion bulking machine at 120 ℃;
(4) and finally, drying at 80 ℃, and cooling to obtain the feed.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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Application publication date: 20200619 |