CN111296494A - Application of forsythia extract in preventing and controlling agricultural pests - Google Patents
Application of forsythia extract in preventing and controlling agricultural pests Download PDFInfo
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- CN111296494A CN111296494A CN202010141543.0A CN202010141543A CN111296494A CN 111296494 A CN111296494 A CN 111296494A CN 202010141543 A CN202010141543 A CN 202010141543A CN 111296494 A CN111296494 A CN 111296494A
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- extract
- agricultural pests
- forsythia
- ethanol
- forsythia suspense
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- Agronomy & Crop Science (AREA)
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- Plant Pathology (AREA)
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Abstract
The invention provides application of a forsythia suspense extract in preventing and controlling agricultural pests, wherein the forsythia suspense extract is an ethanol extract; can be used for preventing and controlling agricultural pests such as armyworm, ostrinia nubilalis and spodoptera frugiperda; is environment-friendly.
Description
Technical Field
The invention belongs to the field of plant-derived pesticides, and particularly relates to application of a forsythia suspense extract to prevention and control of agricultural pests.
Background
Fructus forsythiae is a common Chinese medicinal material, and mainly contains phenylethanoid glycosides, lignans and their glycosides, pentacyclic triterpenes, volatile oil, etc. Pharmacological research considers that the medicine has the effects of resisting bacteria, resisting inflammation, relieving fever, stopping vomiting, promoting urination, strengthening heart, resisting liver damage, easing pain, resisting virus, reducing blood pressure and the like.
The fructus forsythiae is used as a traditional Chinese medicine field, the research on the aspect of agricultural application is less, in agriculture, the research on the activity of fructus forsythiae extracts is mainly concentrated in the sterilization field, for example, fructus forsythiae extracts have good inhibition effects on cotton wilt pathogen, cucumber gray mold and fusarium moniliforme, the inhibition rate of hypha growth is more than 50% (Pan and the like, the inhibition activity of 8 traditional Chinese medicine extracts on plant pathogenic fungi), ethanol extracts of flowers, leaves, branches and fruits (including green forsythia and fructus peruvii) of fructus forsythiae have certain inhibition effects on three bacterial diseases escherichia coli, staphylococcus aureus, bacillus subtilis, 3 fungal diseases penicillium, botrytis cinerea and aspergillus flavus (the comparison analysis of the antibacterial effects of extracts of different parts of fructus forsythiae, the antibacterial activity and the component analysis of ethanol extracts of fructus forsythiae fruits and fructus forsythiae leaves), LC50 values of the tobacco methyl and pseudoglutethic acid methyl fumigant activities of the fructus forsythiae volatile oil are respectively 8.94 and 7.68mg/L, LD50 values of contact poisoning activities are respectively 23.66 and 30.13 g/g, the LD 364-18 g terpene derivatives have certain insecticidal effects on tobacco poisoning effects on tobacco pests (the tobacco poisoning effect of the tobacco) of the tobacco poisoning alcohol and the tobacco poisoning effect of the fructus forsythiae volatile oil on the tobacco poisoning of the tobacco poisoning substances of the tobacco.
Most of the chemical pesticides are non-naturally occurring substances, are not easy to degrade, have long residual period, have destructive effect on natural ecology, are easy to form public nuisance, and even endanger human health. Therefore, it has become urgent to develop a plant-derived pesticide which is highly effective against harmful organisms, safe against non-target organisms, easily decomposed, and does not damage the environment by the decomposition products. The invention mainly researches the application of the forsythia suspense extract in the aspect of pesticides.
Disclosure of Invention
The invention provides a botanical pesticide which is environment-friendly, safe to non-target organisms, easy to decompose and free of damage to the environment by decomposition products. The invention provides the following technical scheme:
the application of the forsythia suspense extract in preventing and controlling agricultural pests is characterized in that the extract is an ethanol extract.
The extraction method of the ethanol extract comprises the following steps: oven drying fructus forsythiae fruit and folium forsythiae in 75 deg.C constant temperature oven to constant weight, pulverizing, sieving, adding 70% ethanol at a ratio of 1: 10-20(g/mL), reflux extracting at 78-82 deg.C for 2-4 hr for three times, filtering, mixing filtrates, and rotary evaporating to concentrate to obtain fructus forsythiae fruit and folium forsythiae ethanol extract.
The agricultural pests are armyworm, corn borer and spodoptera frugiperda.
The forsythia suspense extract is used for preparing the insecticide, and the insecticide is in the dosage forms of missible oil, microemulsion, granules, wettable powder and water dispersible granules.
Compared with the prior art, the invention has the following beneficial effects:
the forsythia suspense extract belongs to a plant source extract, is a plant source pesticide which is environment-friendly, safe to non-target organisms, easy to decompose and harmless to the environment by decomposition products. The forsythia suspense extract is adopted to prevent and control agricultural pests, so that the dosage of chemical agents can be reduced, and the environmental pollution is reduced.
The applicant proves that the forsythia suspense extract has good control effect on agricultural pests such as armyworm, ostrinia nubilalis and spodoptera frugiperda.
Description of the drawings:
FIG. 1 contact killing activity of forsythia suspense extract on armyworm 3-instar larvae
FIG. 2 stomach toxicity Activity of Forsythia suspensa extract on corn borer
FIG. 3 stomach toxicity Activity of Forsythia suspensa extract on Spodoptera frugiperda
Detailed Description
The following detailed description of specific embodiments of the present invention is provided in conjunction with examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1 preparation of forsythia suspense extract
Placing fructus forsythiae and folium forsythiae in a 75 deg.C constant temperature oven, drying to constant weight, pulverizing, sieving, adding 70% ethanol at a ratio of 1: 15(g/mL) respectively, reflux-extracting at 80 deg.C for 3 hr for three times, filtering, mixing filtrates, and rotary evaporating the filtrate to obtain fructus forsythiae and folium forsythiae ethanol extract. Taking fructus forsythiae and fructus forsythiae leaf ethanol extract, and diluting with acetone to constant volume to obtain 1g/mL solution for later use.
Example 2 contact killing Activity of Forsythia suspensa extract on armyworm
The dipping method comprises the following steps: the sample extract prepared in example 1 was diluted with water by 100 times, 20 times, 10 times and 5 times in sequence, 3-instar armyworm larvae of the same size were selected and placed in an armyworm soaking tube, and the tube was soaked in the diluent for 10 seconds, and then taken out, excess liquid medicine was blotted with absorbent paper, and the tube was placed in a petri dish with fresh corn leaves, and the treatment was repeated 3 times, and a blank control was set for 10 test insects. The culture dish is placed in an incubator at 28 ℃ for culture, the death condition of the test insects is investigated after 24 hours, and the death rate is calculated.
TABLE 1 contact killing Activity of Forsythia suspensa extract on armyworm larvae of 3 rd instar
| Treatment of | Number/head of tested insects | Number/head of dead insects | Mortality rate/%) | Corrected mortality% |
| 100 |
30 | 1 | 3.33 | 3.33 |
| 20 |
30 | 3 | 10.00 | 10.00 |
| 10 |
30 | 7 | 23.33 | 23.33 |
| 5 |
30 | 14 | 46.67 | 46.67 |
| CK | 30 | 0 | 0 | 0 |
Contact killing effect of forsythia suspense extract on armyworm is shown in table 1. As can be seen from Table 1, the extract of Forsythia suspensa has a certain contact-killing effect on armyworm. With increasing extract concentration, the contact killing activity increased. The corrected mortality rate for contact killing activity was 46.67% when 1g/mL of the forsythia suspense extract was diluted 5-fold.
Example 3 determination of stomach toxicity Activity of Forsythia suspensa extract on corn borer
Leaf disc method of virus inclusion: the sample extract prepared in example 1 was diluted with water in the order of 100 times, 20 times, 10 times, and 5 times. Making fresh and non-chemical pesticide-used corn leaves into a circular leaf disc with the diameter of 15mm, soaking the leaf disc in the liquid medicine for 3s, taking out the leaf disc, and clamping the leaf disc between two untreated leaf discs with the same diameter after the solvent is volatilized to make the toxic leaf disc. Placing 6 toxic leaf discs in each culture dish at equal intervals, feeding 3-instar larvae of corn borer hungry for 4h, setting blank controls, treating 20 larvae each, placing in an artificial climate box, continuously feeding, and repeating for 3 times. The death number of the larvae of the corn borers is recorded by a 48h check, and the corrected mortality rate is calculated.
TABLE 2 stomach toxicity Activity of Forsythia suspensa extract on corn borer
| Treatment of | Number/head of tested insects | Number/head of dead insects | Mortality rate/%) | Corrected mortality% |
| 100 |
60 | 5 | 8.33 | 5.17 |
| 20 |
60 | 15 | 25.00 | 22.41 |
| 10 |
60 | 22 | 36.67 | 34.48 |
| 5 |
60 | 34 | 56.67 | 55.17 |
| CK | 60 | 2 | 3.33 | 3.33 |
The stomach toxicity of forsythia suspense extract on corn borer is shown in table 2. As can be seen from Table 2, the forsythia suspense extract has a certain stomach toxicity effect on corn borers. The activity increased with increasing extract concentration. The corrected mortality rate for contact killing activity was 55.17% when 1g/mL forsythia suspense extract was diluted 5-fold.
Example 4 measurement of stomach toxicity Activity of Forsythia suspensa extract on Spodoptera frugiperda
The leaf dipping method was performed by diluting the sample extract solution prepared in example 1 with water in the order of 100 times, 20 times, 10 times, and 5 times. Soaking fresh and unused corn leaves in the treatment liquid medicine for 20s, airing, putting into plastic culture dishes, inoculating 1 head of 3-year-old larva hungry for 4h into each culture dish, treating each concentration for 10 heads, repeating for 3 times, and setting blank control. The larval mortality was counted at 48h for each treatment group and the corrected mortality was calculated.
TABLE 3 stomach toxicity Activity of Forsythia suspensa extracts on Spodoptera frugiperda
| Treatment of | Number/head of tested insects | Number/head of dead insects | Mortality rate/%) | Corrected mortality% |
| 100 |
30 | 0 | 0 | 0 |
| 20 |
30 | 2 | 6.67 | 6.67 |
| 10 |
30 | 5 | 16.67 | 16.67 |
| 5 |
30 | 10 | 33.33 | 33.33 |
| CK | 30 | 0 | 0 | 0 |
The stomach toxicity of forsythia suspense extract against spodoptera frugiperda is given in table 3. As can be seen from Table 3, the forsythia suspense extract has a certain stomach toxicity effect on corn borers. The activity increased with increasing extract concentration. The corrected mortality rate for contact killing activity was 33.33% when 1g/mL of the forsythia suspense extract was diluted 5-fold.
The research preliminarily researches the insecticidal activity of the forsythia suspense ethanol extract, and the forsythia suspense ethanol extract has certain stomach toxicity and contact killing activity on armyworm, ostrinia nubilalis and spodoptera frugiperda within a certain concentration range. However, what substance or species produces the effect and what mechanism needs to be further studied.
Claims (6)
1. The application of the forsythia suspense extract in preventing and controlling agricultural pests is characterized in that the extract is an ethanol extract.
2. Use according to claim 1, characterized in that: the extraction method of the ethanol extract comprises the following steps: oven drying fructus forsythiae fruit and folium forsythiae in 75 deg.C constant temperature oven to constant weight, pulverizing, sieving, adding 70% ethanol at a ratio of 1: 10-20(g/mL), reflux extracting at 78-82 deg.C for 2-4 hr for three times, filtering, mixing filtrates, and rotary evaporating to concentrate to obtain fructus forsythiae fruit and folium forsythiae ethanol extract.
3. Use according to claim 1, characterized in that: the agricultural pests are armyworm, corn borer and spodoptera frugiperda.
4. The application of the forsythia suspense extract in preparing the pesticide is characterized in that the extract is an ethanol extract.
5. Use according to claim 3, characterized in that: the agricultural pests are armyworm, corn borer and spodoptera frugiperda.
6. The use according to claim 3, wherein the insecticide is in the form of emulsifiable concentrate, microemulsion, granules.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010141543.0A CN111296494A (en) | 2020-03-03 | 2020-03-03 | Application of forsythia extract in preventing and controlling agricultural pests |
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| CN202010141543.0A CN111296494A (en) | 2020-03-03 | 2020-03-03 | Application of forsythia extract in preventing and controlling agricultural pests |
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| CN111296494A true CN111296494A (en) | 2020-06-19 |
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| CN202010141543.0A Withdrawn CN111296494A (en) | 2020-03-03 | 2020-03-03 | Application of forsythia extract in preventing and controlling agricultural pests |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114451425A (en) * | 2022-03-07 | 2022-05-10 | 四川大学 | Application of fructus forsythiae extract in preventing and treating kiwifruit canker |
-
2020
- 2020-03-03 CN CN202010141543.0A patent/CN111296494A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114451425A (en) * | 2022-03-07 | 2022-05-10 | 四川大学 | Application of fructus forsythiae extract in preventing and treating kiwifruit canker |
| CN114451425B (en) * | 2022-03-07 | 2022-07-01 | 四川大学 | Application of fructus forsythiae extract in preventing and treating kiwifruit canker |
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Application publication date: 20200619 |
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