CN111280179A

CN111280179A - Anti-coccidiosis disinfectant

Info

Publication number: CN111280179A
Application number: CN202010184965.6A
Authority: CN
Inventors: 张丽芳; 王春梅; 费陈忠; 王汝金; 朱建德; 薛飞群; 谷峰; 王霄旸; 程培培; 王米; 张可煜; 刘迎春
Original assignee: Shanghai Veteromaru Research Institute Caas China Animal Health And Epidemiology Center Shanghan Branch Center
Current assignee: Shanghai Veteromaru Research Institute Caas China Animal Health And Epidemiology Center Shanghan Branch Center; Shanghai Veterinary Research Institute CAAS
Priority date: 2020-03-17
Filing date: 2020-03-17
Publication date: 2020-06-16

Abstract

The invention discloses an anti-coccidiosis disinfectant, which is prepared by combining the following components in percentage by mass: 20-80% of chloride and 80-20% of potassium hydrogen persulfate; or the following steps: 40-60% of potassium hydrogen persulfate, 20-60% of chloride, 0-5% of acidifying agent, 0-10% of surfactant, 0-10% of corrosion inhibitor, 0-5% of drying agent and 0-0.2% of indicator; wherein the chloride is sodium chloride or potassium chloride. The invention has the effect of killing coccidian oocysts and can effectively inhibit and kill coccidian non-sporulated oocysts and sporulated oocysts.

Description

Anti-coccidiosis disinfectant

Technical Field

The invention relates to the technical field of disinfectants, in particular to a disinfectant with the functions of inhibiting and killing coccidian oocysts.

Background

Coccidiosis in chickens is a highly contagious parasitic disease with intestinal lesions caused by one or more species of Eimeria in chickens, which can lead to a decrease in the growth rate and feed conversion rate of the chickens, and a decrease in the quality of the eggs. In severe cases, the chickens die. According to statistics, the incidence rate of coccidiosis of the chicken is 50-70%, the outbreak mortality rate is 20-30%, and the economic loss caused by the coccidiosis of the chicken worldwide is 50 billion dollars each year. China is a big chicken-raising country, accounts for 10 percent of the total chicken raising quantity in the world, and the direct and indirect economic loss caused by chicken coccidiosis is more than 30 hundred million RMB each year.

The life history of coccidia can be divided into three stages, namely asexual reproduction stage, sexual reproduction stage and sporulation stage. Wherein the asexual and sexual stages are performed in the animal; the sporogonic stage is accomplished in an external environment.

At present, the prevention and treatment measures for coccidiosis mainly comprise the following two measures: the treatment and prevention measures aiming at the coccidian in-vivo development stage are mainly taken by chemical drugs and vaccines. However, the long-term use of an anticoccidial drug has led to drug resistance that plagues veterinary clinics. In the chicken industry in particular, the drug resistance problem caused by coccidia is more common, and the coccidia drug resistance problem becomes the first large enemy of coccidia resistance in the chicken industry. In addition, due to the diversity of coccidia species and the complexity of the immune mechanism, to date, there has not been any coccidia vaccine that is effective against a variety of pathogenic coccidia and is suitable for use in chicken farms. Failure of immunization often occurs, preventing effective use of vaccine immunoprophylaxis.

Another measure is a disinfection measure against the in vitro development stage of coccidia. Therefore, the occurrence of chicken coccidiosis has a direct relationship with environmental sanitation, namely environmental factors and chicken coccidiosis in Caocandon, Zhangyu, poultry science, 2006 (2): 22 (c). The outbreaks of coccidiosis in chickens are mainly caused by coccidial oocyst contamination in the chicken house environment. The use of disinfectants to control sources of infection in the environment is therefore an effective means. However, coccidial oocysts include both non-sporulated oocysts, which present tough walls, and sporulated oocysts, which present tough walls of oocysts and sporocysts, which provide coccidial oocysts with superior resistance to desiccation and penetration by chemical disinfectants, precisely because of their unique wall structure, which is highly resistant to harsh environmental conditions and general disinfection. Conventional chemical disinfectants such as sodium hydroxide, peracetic acid, glutaraldehyde, benzalkonium bromide, active silver ion solutions, etc. cannot kill sporulated oocysts.

The literature reports very few disinfectants with anticoccidial effects, which are reported only in the 90 th century above under the trade name of spinosad, manufactured by andrd international corporation, uk, and examined science No. 1, developed by the national institute of quarantine science, in the early part of this century. According to the literature, the title spinosad developed by the international british andre is composed mainly of activator (1) and base (2) (waning, yangwu, cinnapple. spinosad killing effect on chicken coccidian oocysts, chinese veterinary journal, 1995, 21 (4): 24-25). The components of the activator are ammonium salt, surfactant and indicator; the main medicine component is sodium hydroxide and an organic pesticide. When the product is used, the ammonium salt and the sodium hydroxide are used for on-site reaction to generate ammonia gas so as to achieve the aim of resisting coccidian oocysts. Therefore, the product has the defects of strong corrosivity and strong irritation. The data of related inspection department No. 1 is only reported in documents (Zhang Shu, Chi Yongcheng, Ma Chaying, etc.. "inspection department No. 1" research on inhibiting in vivo chicken development. inspection and quarantine science, 2007, 17 (3): 15-18), and is not put on the market formally. Therefore, the coccidian-resistant disinfectant with the advantages of effectiveness, safety and small corrosivity is screened and developed, the coccidian sporulated oocysts and non-sporulated oocysts in the environment are effectively killed, and the coccidian-resistant disinfectant has very important significance for effectively controlling the coccidiosis of the chicken.

Oxone, alternative name: potassium monopersulfate triple salt, oxone complex, potassium peroxymonosulfate, abbreviated as PMPS or KMPS. The novel peroxide disinfectant belongs to a novel peroxide disinfectant, is white granular, is easy to dissolve in water, generates various hydrogen peroxide derivatives such as high energy, active oxygen and the like after being dissolved in water, destroys a cell membrane permeability barrier of microorganisms, enables cell contents to run off, generates nascent oxygen (O) and free hydroxyl (-OH) through chain reaction, destroys the cell membrane permeability of the microorganisms, interferes the synthesis of pathogen DNA and RNA, and has good bactericidal performance and inactivation effect on microorganisms such as bacterial propagules, fungi, viruses and the like.

Because the potassium hydrogen persulfate has unique killing effect on microorganisms, scientific researchers in all countries around the world carry out full research and application on the potassium hydrogen persulfate. The DuPont company of America developed potassium hydrogen persulfate composite powder (trade name: Weike, England name Virkon) with potassium hydrogen persulfate as main component in the first century. The composite potassium hydrogen persulfate disinfectant is a fifth generation disinfectant which integrates broad spectrum, high efficiency, safety and environmental protection, and has been increasingly accepted by users of families, hospitals, tap water, aquatic products and livestock and poultry breeding industries (Song Hai Peng. contemporary aquatic products 2015, 40 (03): 64-65). The same point of the product is that the product is a compound powder prepared by taking potassium hydrogen persulfate as a main component and assisting with some auxiliary materials. A plurality of patents such as national invention patent application numbers 201110149685.2, 201810980866.1, 201710327528.3, 201710278811.1, 201511020495.5, 201910709629.6, 2014201410426347.2. The formulas of the patents are different in size, and the formulas are formed by taking potassium hydrogen persulfate as a main component, an acidifier and sodium chloride as synergists and other auxiliary materials as auxiliary materials. The formula comprises the following main components in percentage by mass: 15-65% of potassium hydrogen persulfate, 0-16% of sodium chloride and 2-30% of acidulant comprising citric acid or sulfamic acid and the like. The potassium hydrogen persulfate compound disinfectant can be used for disinfecting livestock and poultry breeding industry, and can kill various bacteria, viruses, molds and fungi such as escherichia coli, staphylococcus aureus, avian influenza, foot-and-mouth disease and the like. However, no coccidium killing effect is found. The literature "Gadelhaq, Sahar M., etc. in vitro activity of natural and chemical products on the project of Eimeria species of animals of foreign Parsitiology, 15February 2018, Vol.251, pp.12-16" shows that the potassium hydrogen persulfate composite powder disinfectant, which is under the trade name Virkon (Virkon), has no killing effect on coccidia and cannot inhibit the sporulation of coccidia oocysts. The food safety and public health center published disinfectant antimicrobial profiles at the Iowa State university (http:// www.cfsph.iastate.edu/pdf/antimicrobial-spectra-of-disc) also showed that Virkon s were not effective against coccidia. Chinese patent application No. cn201010154464.x discloses that disinfectant tablets made with reference to a sanitising disinfectant formulation have the same action and use as sanitising.

In view of the current situation of chicken coccidiosis prevention and treatment and the unique disinfection effect of the potassium hydrogen persulfate, the effort is made to change the prescription to obtain a novel potassium hydrogen persulfate compound disinfectant with coccidiosis resisting effect, safety and low corrosivity, which has very important significance for controlling the chicken coccidiosis.

Disclosure of Invention

The invention aims to overcome the defect that the existing potassium hydrogen persulfate compound disinfectant cannot inhibit and kill coccidium, and provides a disinfectant which can kill coccidium oocysts and further achieve an anti-coccidium effect.

The purpose of the invention is realized by the following technical scheme:

an anti-coccidiosis disinfectant is characterized in that the disinfectant is prepared by combining the following components in percentage by mass:

20 to 80 percent of chloride

80-20% of potassium hydrogen persulfate.

The anti-coccidiosis disinfectant is characterized by being prepared by combining the following components in percentage by mass:

the chloride is sodium chloride or potassium chloride.

The acidulant is a solid acidulant and is selected from one or more of sulfamic acid, malic acid and citric acid;

the surfactant is sodium dodecyl benzene sulfonate;

the corrosion inhibitor is sodium hexametaphosphate, sodium tripolyphosphate or sodium polyphosphate;

the drying agent is anhydrous sodium sulfate or anhydrous magnesium sulfate;

the indicator is amaranth, carmine, daily yellow or lemon yellow.

An application of the disinfectant in resisting coccidiosis features that it is mixed with water to obtain the aqueous solution whose concentration is 1-5% (W/V) for suppressing and killing coccidiosis.

In the prescription screening stage, different prescriptions of disinfectants are prepared by referring to the content ranges of the main components of the potassium hydrogen persulfate compound disinfectants mentioned in the prior patents, other documents and the current commodities, and the more typical bacteria of escherichia coli, staphylococcus aureus, streptococcus equinus and adenovirus are selected for verification of a microorganism killing test, and the results show that prescription samples in the content ranges of the main components of the potassium hydrogen persulfate compound disinfectants mentioned in the patents, documents and the current commodities have better killing effects on escherichia coli, staphylococcus aureus, streptococcus equinus and influenza viruses. The disinfectant samples are added with water to prepare 2% solution to act on the chicken Eimeria tenella non-sporulated oocysts for 2 hours, the influence on sporulation is examined, and the disinfectant is found to not influence the sporulation of the chicken Eimeria tenella oocysts. (the results are shown in Table 1). That is, these formulations of disinfectants are not effective against coccidia. TABLE 1 Effect of Pecten-isoxadified oxone complex disinfectants on chicken Eimeria tenella oocyst sporulation rates

Group of samples	Rate of oocyst sporulation
		Sanitary device	95.4％
Document prescription set 1	96.5％
		Document prescription group 2	96.9％
Document prescription group 3	95.9％
		Untreated control oocyst group	97.1％

(in the table, the recipe 1 of the reference formula (in mass%) 65% potassium hydrogen persulfate, 16% sodium chloride, 5% sulfamic acid and 14% sodium hexametaphosphate, the recipe 2 of the reference formula (in mass%) 50% potassium hydrogen persulfate, 5% malic acid, 5% sulfamic acid, 10% sodium dodecyl sulfate and 30% sodium hexametaphosphate, and the recipe 3 of the reference formula (in mass%) 15% potassium hydrogen persulfate, 10% sodium chloride, 30% sulfamic acid, 15% sodium dodecyl benzene sulfonate and 30% sodium silicate)

When the series of disinfectant samples are applied to a killing test of the microorganism bacillus cereus, the disinfectant is added with water to prepare a 1g/L solution, and different diluents are selected to investigate the effect of the disinfectant on the bacillus cereus, so that the disinfectant samples taking distilled water as the diluents have no killing effect on the bacillus cereus by accident; the disinfectant sample using normal saline as diluent has a certain killing effect on bacillus cereus (see table 2).

TABLE 2 results of the disinfectant on Bacillus cereus with different diluents

This unexpected result is suggested: sodium chloride plays a decisive role in the killing of bacillus cereus by the disinfectant, so that the dosage of the sodium chloride in the prescription of the potassium hydrogen persulfate compound disinfectant is attempted to be increased so as to achieve the killing effect on coccidia. Through repeated tests, the prescription is continuously screened and optimized, and finally, the prescription of the disinfectant with better inhibition and killing effects on coccidia is screened.

The invention aims to overcome the defects that the existing potassium hydrogen persulfate compound disinfectant cannot inhibit and kill coccidium and the defects of strong corrosivity and strong irritation of the existing coccidium disinfectant, and develops a safe disinfectant which has small corrosivity and can kill coccidium oocysts to achieve the effect of resisting coccidium.

Compared with the prior art, the composition can kill bacteria and viruses such as escherichia coli, staphylococcus aureus, avian influenza, foot-and-mouth disease and the like shared by the existing or literature-reported oxone-potassium persulfate compound disinfectants on the market. The most outstanding advantage is that the composition of the invention can effectively inhibit coccidian non-sporulated oocysts and sporulated oocysts.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the invention to other conventional forms of its implementation.

Example 1

An anti-coccidiosis disinfectant comprises the following raw materials in percentage by mass: 20% of potassium hydrogen persulfate and 80% of sodium chloride. Mixing the two, and sieving with 50 mesh sieve to obtain the final product.

Example 2

An anti-coccidiosis disinfectant comprises the following raw materials in percentage by mass: 80% of potassium hydrogen persulfate and 20% of sodium chloride. Mixing the two, and sieving with 50 mesh sieve to obtain the final product.

Example 3

An anti-coccidiosis disinfectant comprises the following raw materials in percentage by mass: 60% of potassium hydrogen persulfate, 20% of sodium chloride, 1% of sulfamic acid, 4% of sodium dodecyl benzene sulfonate, 10% of sodium hexametaphosphate and 5% of anhydrous magnesium sulfate. Mixing, and sieving with 50 mesh sieve to obtain the final product.

Example 4

An anti-coccidiosis disinfectant comprises the following raw materials in percentage by mass: 40% of potassium hydrogen persulfate, 50% of potassium chloride, 1% of malic acid, 4.8% of sodium tripolyphosphate, 4% of anhydrous sodium sulfate and 0.2% of amaranth. Mixing, and sieving with 50 mesh sieve to obtain the final product.

Example 5

An anti-coccidiosis disinfectant comprises the following raw materials in percentage by mass: 50% of potassium hydrogen persulfate, 30% of potassium chloride, 8% of citric acid, 2% of sulfamic acid, 9.8% of sodium polyphosphate and 0.2% of lemon yellow. Mixing, and sieving with 50 mesh sieve to obtain the final product.

Example 6

An anti-coccidiosis disinfectant comprises the following raw materials in percentage by mass: 45% of potassium hydrogen persulfate, 35% of sodium chloride, 3% of malic acid, 2% of sulfamic acid, 10% of sodium dodecyl benzene sulfonate, 4.8% of anhydrous magnesium sulfate and 0.2% of carmine. Mixing, and sieving with 50 mesh sieve to obtain the final product.

Example 7

An anti-coccidiosis disinfectant comprises the following raw materials in percentage by mass: 55% of potassium hydrogen persulfate, 35% of sodium chloride, 2% of sulfamic acid, 3% of sodium dodecyl benzene sulfonate, 4.8% of anhydrous magnesium sulfate and 0.2% of sunset yellow. Mixing, and sieving with 50 mesh sieve to obtain the final product.

Example 8

Effect of disinfectant on sporulation of Eimeria tenella oocysts

1 materials and methods

1.1 disinfectant

Examples 1 to 7, water was added to the samples prepared in example 1 to prepare 2 to 10% (w/v) aqueous solutions just before use.

1.2 methods

Suspending the eimeria tenella non-sporulated oocysts and the disinfectant aqueous solution prepared in the 1.1 in a 50mL centrifuge tube for 2 hours respectively, and suspending the blank control group by using deionized water and the oocysts for the same time. Then washing with water, centrifuging at 3000rpm for 8min, discarding the liquid layer, repeating the washing centrifugation operation three times to remove the residual disinfectant, adding 10ml of 2.5% potassium dichromate solution to the precipitate for suspension, shaking the sporulation culture in a shaking table at 28 ℃ for 72h, observing 100 oocysts with a sampling microscope, calculating the sporulation rate and the sporulation inhibition rate of the oocysts, and evaluating the sporulation inhibition effect of different disinfectants and anticoccidial drugs on fresh oocysts (Zhang, Ningshu, Liu Hua, etc.. screening of drugs inhibiting the sporulation of avian coccidium oocysts [ J ]. China Veterinary journal, 1999, 1: 10-11; Wenflue, Zhang, Jia, Zhenghe, etc.. the influence of different drugs and disinfectants on the sporulation of rabbit coccidium oocysts [ J ]. laboratory and comparative medicine, 2016, 36 (4): 301. basic 305; Daugchies A, SERet, SERX J, development. 2002, 103(4): 299-308.), and the results are shown in Table 3.

The oocyst sporulation rate (%) is the number of sporulated oocysts/(number of sporulated oocysts + number of non-sporulated oocysts) × 100%;

the sporulation inhibition ratio (%) was ═ average non-sporulation ratio in the treated group-average non-sporulation ratio in the control group)/(1-average non-sporulation ratio in the control group) × 100%.

The results show that the disinfectants in all the formulas have obvious killing effect on the non-sporulated oocysts.

TABLE 3 sporulation-inhibiting effect of disinfectant on non-sporulated oocysts

Disinfectant/blank group	Disinfectant aqueous solution concentration (w/v)	Rate of oocyst sporulation	Rate of oocyst inhibition
				EXAMPLE 1 disinfectant	5％	8.5％	90.9
EXAMPLE 2 disinfectant	5％	7.2％	92.3
				EXAMPLE 3 disinfectant	3％	1.6％	98.3
EXAMPLE 4 disinfectant	1％	10.8％	88.5
				EXAMPLE 5 disinfectant	2％	4.2％	95.5
EXAMPLE 6 disinfectant	1％	12.5％	86.6
				EXAMPLE 7 disinfectant	1％	15.3％	83.7
Control group		93.7％

Example 9

Influence of disinfectant on sporulation of non-sporulated coccidian oocysts of chickens of different genera

1 materials and methods

1.1 disinfectant

The sample prepared in example 7 was prepared as a 2% (w/v) aqueous solution by adding water immediately before use.

1.2 methods

Each coccidian non-sporulated oocyst was suspended in 50mL centrifuge tubes for 2h with aqueous disinfectant solution, and the blank control group was suspended in deionized water and fresh oocysts for the same time. Then washing with water, centrifuging at 3000rpm for 8min, discarding the liquid layer, repeating the washing and centrifuging operation for three times to remove the residual disinfectant, adding 10ml of 2.5% potassium dichromate solution into the precipitate for suspension, shaking and sporulating and culturing for 72h at a constant temperature of 28 ℃, sampling and observing 100 oocysts by a microscope, calculating the sporulation rate and the sporulation inhibition rate of the oocysts, and evaluating the sporulation inhibition and killing effects of different disinfectants and anticoccidial drugs on the fresh oocysts (Zhang Longnow, Ningshen, Liukai Huai Huan, etc., screening of chicken coccidium oocyst killing inhibition and killing drugs [ J ]. Chinese veterinary magazine, 1999, 1: 10-11; Wenfu, Zhang Jia, Zheng, Heng, etc., the influence of different drugs and disinfectants on the sporulation of rabbit coccidium oocysts [ J ]. experimental and comparative medicine, 2016, 36 (4): 301-, development and application of a standardized assessment for chemical discovery of coccodia oocysts [ J ]. Veterimental Parasitology, 2002, 103 (4): 299-308.), and the results are shown in Table 4.

The result shows that the disinfectant has obvious killing effect on fresh coccidian oocysts.

TABLE 4 sporulation-inhibiting effect of disinfectant on various chicken coccidia non-sporulated oocysts

Genus of Coccidium species	Rate of oocyst sporulation	Rate of oocyst inhibition
			Eimeria tenella	4.3％	95.4％
Eimeria necatrix	6.2％	93.4％
			Eimeria maxima	5.3％	94.4％
Eimeria acervulina	2.6％	97.2％
			Control	94.5％

Example 10

Test of inhibition and killing effect of disinfectant on chicken Eimeria tenella sporulated oocysts

1 materials and methods

1.1 animals

1 day old of Pudong yellow feather cocks.

1.2 disinfectant and sporulation oocyst treatment

A disinfectant: the disinfectant samples prepared in example 7 were each prepared as a 2% (W/V) aqueous solution by adding water just before use.

Sporulation oocyst treatment: suspending the Eimeria tenella sporulated oocysts and disinfectant aqueous solution in a 50mL centrifuge tube for 2h respectively, and suspending blank control sporulated oocysts by using deionized water for the same time. Washing with water, centrifuging at 3000rpm for 8min, discarding the liquid layer, centrifuging the precipitate with water for three times to remove residual disinfectant, and adding deionized water to the precipitate to obtain solution containing oocyst solution of 80000 oocysts/ml.

1.3.1 Experimental groups

40 1-day-old Pudong yellow-feathered cocks were used in the test and were randomly grouped into 4 groups after being raised in a healthy animal house for 14 days. Each group had 10. One group was a healthy control group, an infected control group and two disinfectant groups.

1.3.2 coccidian infection

After grouping is completed, 80000 sporulated oocysts are inoculated on the craw of the other groups except for a healthy control group, wherein blank control sporulated oocysts are inoculated on an infected control group; the disinfectant group was inoculated with disinfectant-treated sporulated oocysts.

1.4 clinical observations

During the test period, the feeding condition, mental state, morbidity, bloody stool and the like of the feed and drinking water of the test chicken are observed and recorded every day. And collecting the excrements of each group of chickens for counting the excrements oocysts in the fifth, sixth and seventh days after infection, and recording the highest value in the calculation of ACI. The death condition of the test chicken is observed every day during the test period, and the autopsy verification is well done. And on the eighth day, weighing each group respectively, killing all the groups, and observing and recording the weight increment and the caecum pathological change conditions of the test chickens.

1.5 evaluation of drug efficacy

1.5.1 mortality: the number of chickens killed by infection with coccidia was a percentage of the number of test chickens.

1.5.2 relative rate of gain: percentage of average weight gain of test chickens in each infected group to that of healthy control group.

1.5.3 Blind bowel variability values: the cecal lesion score lesion value was calculated as the average lesion score of the full panel by Johnson and Reid (1970).

1.5.4 oocyst value groups of cecal contents were homogenized and the number of oocysts per gram of cecal contents (OPG) was calculated using the Mc-Master's method. The oocyst ratio (number of oocysts in the negative control group or administration group ÷ number of oocysts in the positive control group) × 100%. If the ratio of the oocysts is 0-1%, the value of the oocysts is 0; the ratio of the oocysts is 2 to 25 percent, and the value of the oocysts is 5; the ratio of the oocysts is 26 to 50 percent, and the value of the oocysts is 10; the ratio of the oocysts is 51 to 75 percent, and the value of the oocysts is 20; the ratio of the oocysts is 6-100 percent, and the value of the oocysts is 40.

1.5.5 anticoccidial index (ACI) (mc. loughlin.1970): ACI ═ (relative rate of weight gain + survival) - (lesion value + oocyst value).

1.5.6 criterion ACI was found to be ineffective below 120, ineffective between 120 and 160, moderate at 160 and effective above 180 (compilation of veterinary test specifications, 2001).

1.6 results

The results show that when the oocysts treated by the disinfectant (disinfectant group) and the oocysts not treated (infected control group) are inoculated to chicks, the anticoccidial indexes of the two groups are obviously different, and the anticoccidial index of the disinfectant group achieves the effect of high-efficiency anticoccidial drugs. The disinfectant has obvious killing effect on the chicken Eimeria tenella sporulated oocysts.

TABLE 5 results of the test of the killing effect of the disinfectant on the sporulated oocysts of Eimeria tenella

Claims

1. The anti-coccidiosis disinfectant is characterized by being prepared from the following components in percentage by mass:

20 to 80 percent of chloride

80-20% of potassium hydrogen persulfate.

2. The anti-coccidiosis disinfectant is characterized by being prepared from the following components in percentage by mass:

40-60 percent of potassium hydrogen persulfate

20 to 60 percent of chloride

0 to 5 percent of acidifier

0 to 10 percent of surfactant

0 to 10 percent of corrosion inhibitor

0 to 5 percent of drying agent

0-0.2% of indicator.

3. The anti-coccidial disinfectant of claim 1 or 2, wherein the chloride is sodium chloride or potassium chloride.

4. The anti-coccidial disinfectant as claimed in claim 2, wherein the acidifier is a solid acidifier selected from one or more of sulfamic acid, malic acid and citric acid; the surfactant is sodium dodecyl benzene sulfonate; the corrosion inhibitor is sodium hexametaphosphate, sodium tripolyphosphate or sodium polyphosphate; the drying agent is anhydrous sodium sulfate or anhydrous magnesium sulfate; the indicator is amaranth, carmine, daily yellow or lemon yellow.

5. The disinfectant of claim 1 or 2 for use against coccidia is characterized in that it is added with water to prepare an aqueous solution with a concentration ranging from 1 to 5% for application to coccidia-inhibiting oocysts.