CN111253951B - Preparation process of biological soil conditioner for reducing soil acidity - Google Patents
Preparation process of biological soil conditioner for reducing soil acidity Download PDFInfo
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Abstract
The invention discloses a biological soil conditioner for reducing soil acidity and a preparation process thereof, and relates to the technical field of agriculture. The preparation method comprises the steps of oxidizing bacterial cellulose, mixing the oxidized bacterial cellulose with collagen and nano bentonite for reaction to obtain a composite material, reacting the composite material, freeze-drying the composite material, reacting the composite material with a modifier to obtain porous microspheres treated by the modifier, mixing the porous microspheres treated by the modifier with modified sodium lignosulfonate cultured by mixed fungi, stirring the mixture in a 90% ethanol solution for reaction, filtering, drying, and adding potassium humate to obtain the biomass conditioner. The biomass conditioner prepared by the invention can effectively adjust the pH value of acid soil and has excellent adsorption effect on metal ions.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a preparation process of a biological soil conditioner for reducing soil acidity.
Background
Soil acidification refers to a process in which a soil absorptive complex receives a certain amount of exchangeable hydrogen ions or aluminum ions to leach alkaline (salt) ions in soil. The soil contains a large amount of aluminum hydroxide, and after the soil is acidified, the weathering of primary and secondary minerals containing aluminum in the soil can be accelerated to release a large amount of aluminum ions, so that an aluminum compound which can be absorbed by plants is formed, and the plants can be poisoned and even die due to long-term and excessive aluminum absorption. Soil acidification can change the soil structure, cause soil impoverishment and ridged, influence the normal development of plants, induce plant diseases and insect pests, reduce the yield of crops, and the strong acid environment can also accelerate the filtration of toxic metals to the surrounding water body.
The soil acidity concept can be divided into two categories, one being active and one being latent. The active acid is a direct reflection of the hydrogen ion concentration in the soil solution, and the strength of the active acid is expressed by pH, and the smaller the soil pH is, the stronger the soil active acid is. Latent acids are defined by hydrogen ions and aluminum ions in an exchange state, and when the ions are in an adsorption state, the latent acids do not show out, and when the ions are exchanged into a soil solution, the hydrogen ions increase in concentration to show acidity, and the process is reversible.
The soil conditioner is a product which is prepared by using agricultural water-retaining agent and natural peat or other organic matters rich in organic matters and humic acid as main raw materials and biological active ingredients and nutrient elements as auxiliary materials through a scientific process, and has three remarkable soil conditioning performances of water retention, fertilizer increase and air permeability. The soil hardening can be broken, the soil can be loosened, the soil air permeability can be improved, the soil volume weight can be reduced, the soil microbial activity can be promoted, and the soil fertilizer water permeability can be enhanced; the fertilizer has the functions of improving soil, controlling desert, preserving water, resisting drought, enhancing the disease resistance of crops, improving the yield of the crops, improving the quality of agricultural products, recovering the original ecology of the crops and the like, and greatly improves the survival rate of tree planting and the yield of the agricultural products; improve the quality of agricultural and forestry products and restore the natural appearance of the agricultural and forestry products.
The existing biological soil conditioner generally adopts various microbial strains, organic fertilizers, humic acid, mineral substances, medium trace elements and the like, and some soil conditioners are added with new components such as Chinese herbal medicines, amino acids and the like, but the existing soil conditioners can not effectively treat acidified soil, and the treated soil is subjected to salinization to a certain degree due to good water retention of the soil conditioners, so that the soil conditioner with the functions of controlling soil acidification, good metal ion immobilization and wide market prospect is realized.
Disclosure of Invention
Aiming at the problems that the soil conditioner in the prior art has poor metal ion adsorption performance and poor soil acidity control, the invention provides a biological soil conditioner for reducing soil acidity and a preparation process thereof.
In order to achieve the purpose, the invention provides the following technical scheme:
the biological soil conditioner for reducing soil acidity is characterized by mainly comprising the following raw material components in parts by weight: 20-32 parts of porous microspheres, 5-8 parts of potassium humate, 4-8 parts of mixed bacteria and 3-7 parts of modifier, wherein the porous microspheres contain bacterial cellulose and collagen and have excellent water retention performance, after the porous microspheres are added into a soil conditioner, the water retention performance of a product can be effectively improved, and meanwhile, due to the addition of the modifier, metal ions can be adsorbed between bentonite layers added into the porous microspheres, and the adsorbability of the porous microspheres in the later period is improved.
The biological soil conditioner for reducing soil acidity is characterized by further comprising the following raw material components in parts by weight: 10-12 parts of modified sodium lignosulfonate, wherein the modified sodium lignosulfonate can be added and cultured together with mixed fungi in the preparation process of a product, so that a large amount of mixed fungi are adsorbed in the structure of the modified sodium lignosulfonate, and after the modified sodium lignosulfonate is added into the product, the modified sodium lignosulfonate can be adsorbed to the surface of sodium bentonite in the porous microspheres under the action of the fungi and electrostatic force, and the surface of the adsorbed sodium bentonite has certain hydrophobicity.
The porous microspheres are prepared by mixing and reacting bacterial cellulose, collagen and sodium bentonite, wherein the mixed bacteria are acetobacter xylinum and bacillus pasteurianus according to the mass ratio of 1: 2 mixing the components.
Preferably, the modifier is a mixture of potassium chloride, calcium chloride and hexadecyl trimethyl ammonium bromide, and the modified sodium lignosulfonate is obtained by modifying sodium lignosulfonate with 1, 6-dibromohexane.
As optimization, the biological soil conditioner comprises the following raw material components in parts by weight: 30 parts of porous microspheres, 5 parts of potassium humate, 6 parts of mixed fungi, 5 parts of a modifier and 8 parts of modified sodium lignosulfonate.
As optimization, the preparation process of the biological soil conditioner for reducing soil acidity mainly comprises the following preparation steps:
(1) oxidizing bacterial cellulose, mixing the oxidized bacterial cellulose with collagen and sodium bentonite for reaction to obtain a composite material, reacting the composite material, and freeze-drying the composite material;
(2) mixing the substance obtained in the step (1) and a modifier in water for reaction, filtering and drying;
(3) mixing modified sodium lignosulfonate with mixed fungi in a culture solution, culturing, filtering, and drying;
(4) reacting the substance obtained in the step (2) with the substance obtained in the step (3) in an ethanol solution, filtering and drying to obtain a blank, and mixing the blank with potassium humate;
(5) and (4) performing index analysis on the substance obtained in the step (4).
As optimization, the preparation process of the biological soil conditioner for reducing soil acidity mainly comprises the following preparation steps:
(1) mixing bacterial cellulose and water according to the mass ratio of 1: 50-1: 60, adding potassium periodate which is 0.1-0.2 times of the mass of the bacterial cellulose, stirring for reaction, performing suction filtration to obtain pretreated bacterial cellulose, mixing the pretreated bacterial cellulose and an ethylene glycol solution according to the mass ratio of 1: 30-1: 40, performing suction filtration, washing, and drying to obtain modified bacterial cellulose; mixing collagen and water according to a mass ratio of 1: 100-1: 120, adding modified bacterial cellulose with the mass 1-2 times of that of the collagen and sodium bentonite with the mass 0.3-0.7 time of that of the collagen, stirring for reaction, performing suction filtration, washing, and freeze drying;
(2) mixing the substance obtained in the step (1) with a modifier according to a mass ratio of 6: 1-8: 1, mixing, adding water with the mass being 3-5 times that of the substance obtained in the step (1), stirring for reaction, filtering, and freeze-drying;
(3) mixing modified sodium lignosulfonate with mixed fungi in a mass ratio of 2: 1-3: 1, mixing, adding a culture solution with the mass 8-10 times that of the modified sodium lignosulfonate, mixing and culturing for 5-7 days, filtering, and drying;
(4) mixing the substance obtained in the step (2) with the substance obtained in the step (3) according to a mass ratio of 2: 1-3: 1, adding an ethanol solution which is 6-10 times of the mass of the substance obtained in the step (2), stirring for reaction, filtering, drying to obtain a blank, and mixing the blank with potassium humate according to a mass ratio of 10: 1-12: 1, mixing;
(5) and (4) performing index analysis on the substance obtained in the step (4).
Preferably, the modified sodium lignosulfonate in the step (3) is prepared by mixing sodium lignosulfonate and water in a mass ratio of 1: 10-1: 12, adding 1, 6-dibromohexane accounting for 0.8-1.2 times of the mass of the sodium lignosulfonate and potassium iodide accounting for 0.08-0.10 times of the mass of the sodium lignosulfonate, stirring for reaction, extracting with petroleum ether, filtering, removing an oil phase, and freeze-drying to obtain the modified sodium lignosulfonate.
As optimization, the culture solution in the step (3) is prepared by mixing sucrose and beef extract according to a mass ratio of 4: 1, mixing and adding citric acid with the mass of 0.01-0.02 time of that of the cane sugar, sodium hydrogen phosphate with the mass of 0.02-0.04 time of that of the cane sugar, agar with the mass of 0.2-0.3 time of that of the cane sugar, ethanol with the mass of 0.1-0.2 time of that of the cane sugar and water with the mass of 10-20 times of that of the cane sugar, and stirring and mixing to obtain a culture solution.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the invention, sodium bentonite is added during the preparation of the soil conditioner, and the modifier is used for modifying the porous microspheres containing the sodium bentonite, firstly, the added sodium bentonite has good water retention performance, after the sodium bentonite is added into a product, the water retention performance of the soil conditioner can be effectively improved, secondly, after the modifier is used for modifying the porous microspheres containing the sodium bentonite, sodium ions in the sodium bentonite can be replaced by metal ions in the modifier, and when the soil is acidic, the metal ions absorbed in the bentonite can be exchanged with hydrogen ions, so that the content of the hydrogen ions in the soil is reduced, and further the acidity of the soil is reduced;
(2) the invention uses modified sodium lignosulfonate when preparing soil conditioner, and adds the modified sodium lignosulfonate and mixed fungi into porous microspheres after being cultured together, on one hand, the modified sodium lignosulfonate can be adsorbed and fixed in a network structure of the modified sodium lignosulfonate after being cultured together with the mixed fungi, and can be adsorbed and fixed on the surface of sodium bentonite modified by a modifier in the porous microspheres under the action of electrostatic force after being added into products, and the surface of the modified sodium bentonite presents certain hydrophobicity, so that bacterial colonies can be fixed around the modified sodium bentonite, thereby preventing mass propagation of strains in the products, on the other hand, when the products absorb water, the modified sodium bentonite absorbs water and expands, a coating film formed by the modified sodium lignosulfonate is expanded, and the modified sodium lignosulfonate is dispersed in the porous microspheres, the exposure of active groups in the modified sodium lignosulfonate can improve the adsorption performance of metal ions of the product and prevent the salinization of soil, and the exposure of bacillus pasteurianus in the modified sodium lignosulfonate can decompose urea in the soil to form carbonate ions, and the carbonate ions form precipitates on the metal ions adsorbed by the modified sodium lignosulfonate and are fixed in a three-dimensional network structure of the modified sodium lignosulfonate, so that the acid in the soil can be neutralized, and the acidity of the soil can be reduced.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
To illustrate the method of the present invention more clearly, the following examples are given, and the method for testing each index of the soil acidity reducing biological soil conditioner prepared in the following examples is as follows:
acid control: the biological soil conditioner for reducing the soil acidity obtained in each example and the comparative product are sprayed on the soil surface with the pH value of 5 in an amount of 100g/m < 2 >, and the soil pH value after 24 hours of use is tested;
metal ion adsorption: the biological soil conditioner for reducing soil acidity obtained in each example and the comparative example were put in an aluminum ion solution with a concentration of 3mol/L at an amount of 2g/L, and the aluminum ion concentration after 6 hours of use was measured.
Example 1:
the biological soil conditioner for reducing soil acidity comprises the following raw material components in parts by weight: 30 parts of porous microspheres, 5 parts of potassium humate, 6 parts of mixed fungi, 5 parts of a modifier and 8 parts of modified sodium lignosulfonate.
A preparation process of a biological soil conditioner for reducing soil acidity mainly comprises the following preparation steps:
(1) mixing bacterial cellulose and water according to the mass ratio of 1: 60, mixing the mixture in a beaker, adding potassium periodate which is 0.1-0.2 times of the mass of the bacterial cellulose into the beaker, stirring and reacting for 5 hours at the temperature of 50 ℃ and the rotation speed of 300r/min, performing suction filtration to obtain pretreated bacterial cellulose, mixing the pretreated bacterial cellulose and a glycol solution with the mass fraction of 10% according to the mass ratio of 1:40, stirring and reacting for 3 hours at the temperature of 40 ℃ and the rotation speed of 320r/min, performing suction filtration to obtain a modified bacterial cellulose blank, washing the modified bacterial cellulose blank with deionized water for 6 times, and performing freeze drying to obtain modified bacterial cellulose; mixing collagen and water according to a mass ratio of 1: 120, adding modified bacterial cellulose 2 times of collagen and sodium bentonite 0.5 times of the mass of the collagen into a mixture of the collagen and water, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 320r/min, carrying out suction filtration to obtain a porous microsphere blank, washing the porous microsphere blank with deionized water for 3 times, and freeze-drying;
(2) mixing the substance obtained in the step (1) and a modifier according to a mass ratio of 8: 1, mixing, adding water which is 5 times of the mass of the substance obtained in the step (1), stirring and reacting for 3 hours at the temperature of 50 ℃ and the rotating speed of 320r/min, filtering, and freeze-drying;
(3) mixing modified sodium lignosulfonate with mixed fungi in a mass ratio of 3: 1, mixing the materials in a culture dish, adding a culture solution with the mass 8-10 times that of the modified sodium lignosulfonate into the culture dish, performing mixed culture at the temperature of 30 ℃ for 7 days, filtering, and drying at the temperature of 35 ℃;
(4) mixing the substance obtained in the step (2) with the substance obtained in the step (3) according to a mass ratio of 3: 1, mixing the mixture in a reaction kettle, adding an ethanol solution which is 10 times of the mass of the substance obtained in the step (2) and has the mass fraction of 90%, stirring and reacting for 5 hours at the temperature of 30 ℃ and the rotating speed of 400r/min, filtering to obtain a filter cake, drying the filter cake at the temperature of 35 ℃ to obtain a blank, and mixing the blank and potassium humate according to the mass ratio of 12: 1, mixing;
(5) and (4) performing index analysis on the substance obtained in the step (4).
Preferably, the modified sodium lignosulfonate in the step (3) is prepared by mixing sodium lignosulfonate and water in a mass ratio of 1: 12, adding 1, 6-dibromohexane accounting for 1.2 times of the mass of the sodium lignosulfonate and potassium iodide accounting for 0.10 time of the mass of the sodium lignosulfonate into a mixture of the sodium lignosulfonate and water, stirring and reacting for 5 hours at the temperature of 40 ℃ and the rotating speed of 300r/min, extracting with petroleum ether, filtering, removing an oil phase to obtain a modified sodium lignosulfonate blank, and freeze-drying the modified sodium lignosulfonate blank to obtain the modified sodium lignosulfonate.
As optimization, the culture solution in the step (3) is prepared by mixing sucrose and beef extract according to a mass ratio of 4: 1, adding citric acid with the mass of 0.02 time that of the sucrose, sodium hydrogen phosphate with the mass of 0.04 time that of the sucrose, agar with the mass of 0.3 time that of the sucrose, ethanol with the mass of 0.2 time that of the sucrose and water with the mass of 20 times that of the sucrose into the mixture of the sucrose and the beef extract, and stirring and mixing to obtain a culture solution.
Optimally, the modifier in the step (2) is prepared by mixing potassium chloride and calcium chloride according to the mass ratio of 1: 2, mixing, adding hexadecyl trimethyl ammonium bromide which is 0.1 time of the mass of the potassium chloride, stirring and mixing to obtain the modifier.
Preferably, the mixed fungus in the step (3) is prepared by mixing bacillus pasteurii and acetobacter xylinum according to a mass ratio of 3: 1, mixing to obtain mixed fungi.
Example 2:
the biological soil conditioner for reducing soil acidity comprises the following raw material components in parts by weight: 30 parts of porous microspheres, 5 parts of potassium humate, 6 parts of mixed fungi, 5 parts of a modifier and 8 parts of modified sodium lignosulfonate.
A preparation process of a biological soil conditioner for reducing soil acidity mainly comprises the following preparation steps:
(1) mixing bacterial cellulose and water according to the mass ratio of 1: 60, mixing the mixture in a beaker, adding potassium periodate which is 0.1-0.2 times of the mass of the bacterial cellulose into the beaker, stirring and reacting for 5 hours at the temperature of 50 ℃ and the rotation speed of 300r/min, performing suction filtration to obtain pretreated bacterial cellulose, mixing the pretreated bacterial cellulose and a glycol solution with the mass fraction of 10% according to the mass ratio of 1:40, stirring and reacting for 3 hours at the temperature of 40 ℃ and the rotation speed of 320r/min, performing suction filtration to obtain a modified bacterial cellulose blank, washing the modified bacterial cellulose blank with deionized water for 6 times, and performing freeze drying to obtain modified bacterial cellulose; mixing collagen and water according to a mass ratio of 1: 120, adding modified bacterial cellulose 2 times of collagen into a mixture of the collagen and water, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 320r/min, carrying out suction filtration to obtain a porous microsphere blank, washing the porous microsphere blank with deionized water for 3 times, and freeze-drying;
(2) mixing the substance obtained in the step (1) and a modifier according to a mass ratio of 8: 1, mixing, adding water which is 5 times of the mass of the substance obtained in the step (1), stirring and reacting for 3 hours at the temperature of 50 ℃ and the rotating speed of 320r/min, filtering, and freeze-drying;
(3) mixing modified sodium lignosulfonate with mixed fungi in a mass ratio of 3: 1, mixing the materials in a culture dish, adding a culture solution with the mass 8-10 times that of the modified sodium lignosulfonate into the culture dish, performing mixed culture at the temperature of 30 ℃ for 7 days, filtering, and drying at the temperature of 35 ℃;
(4) mixing the substance obtained in the step (2) with the substance obtained in the step (3) according to a mass ratio of 3: 1, mixing the mixture in a reaction kettle, adding an ethanol solution which is 10 times of the mass of the substance obtained in the step (2) and has the mass fraction of 90%, stirring the mixture for 5 hours at the temperature of 30 ℃ and the rotating speed of 400r/min, filtering the mixture to obtain a filter cake, drying the filter cake at the temperature of 35 ℃ to obtain a blank, and mixing the blank and potassium humate according to the mass ratio of 12: 1, mixing;
(5) and (4) performing index analysis on the substance obtained in the step (4).
Preferably, the modified sodium lignosulfonate in the step (3) is prepared by mixing sodium lignosulfonate and water in a mass ratio of 1: 12, adding 1, 6-dibromohexane accounting for 1.2 times of the mass of the sodium lignosulfonate and potassium iodide accounting for 0.10 time of the mass of the sodium lignosulfonate into a mixture of the sodium lignosulfonate and water, stirring and reacting for 5 hours at the temperature of 40 ℃ and the rotating speed of 300r/min, extracting with petroleum ether, filtering, removing an oil phase to obtain a modified sodium lignosulfonate blank, and freeze-drying the modified sodium lignosulfonate blank to obtain the modified sodium lignosulfonate.
As optimization, the culture solution in the step (3) is prepared by mixing sucrose and beef extract according to a mass ratio of 4: 1, adding citric acid with the mass of 0.02 time that of the sucrose, sodium hydrogen phosphate with the mass of 0.04 time that of the sucrose, agar with the mass of 0.3 time that of the sucrose, ethanol with the mass of 0.2 time that of the sucrose and water with the mass of 20 times that of the sucrose into the mixture of the sucrose and the beef extract, and stirring and mixing to obtain a culture solution.
Optimally, the modifier in the step (2) is prepared by mixing potassium chloride and calcium chloride according to the mass ratio of 1: 2, mixing, adding hexadecyl trimethyl ammonium bromide which is 0.1 time of the mass of the potassium chloride, stirring and mixing to obtain the modifier.
Preferably, the mixed fungus in the step (3) is prepared by mixing bacillus pasteurii and acetobacter xylinum according to a mass ratio of 3: 1, mixing to obtain mixed fungi.
Example 3:
the biological soil conditioner for reducing soil acidity comprises the following raw material components in parts by weight: 30 parts of porous microspheres, 5 parts of potassium humate, 6 parts of mixed fungi and 5 parts of modifier.
A preparation process of a biological soil conditioner for reducing soil acidity mainly comprises the following preparation steps:
(1) mixing bacterial cellulose and water according to the mass ratio of 1: 60, mixing the mixture in a beaker, adding potassium periodate which is 0.1-0.2 times of the mass of the bacterial cellulose into the beaker, stirring and reacting for 5 hours at the temperature of 50 ℃ and the rotation speed of 300r/min, performing suction filtration to obtain pretreated bacterial cellulose, mixing the pretreated bacterial cellulose and a glycol solution with the mass fraction of 10% according to the mass ratio of 1:40, stirring and reacting for 3 hours at the temperature of 40 ℃ and the rotation speed of 320r/min, performing suction filtration to obtain a modified bacterial cellulose blank, washing the modified bacterial cellulose blank with deionized water for 6 times, and performing freeze drying to obtain modified bacterial cellulose; mixing collagen and water according to a mass ratio of 1: 120, adding modified bacterial cellulose 2 times of collagen and sodium bentonite 0.5 times of the mass of the collagen into a mixture of the collagen and water, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 320r/min, carrying out suction filtration to obtain a porous microsphere blank, washing the porous microsphere blank with deionized water for 3 times, and freeze-drying;
(2) mixing the substance obtained in the step (1) and a modifier according to a mass ratio of 8: 1, mixing, adding water which is 5 times of the mass of the substance obtained in the step (1), stirring and reacting for 3 hours at the temperature of 50 ℃ and the rotating speed of 320r/min, filtering, and freeze-drying;
(3) mixing the substances obtained in the step (2) with mixed fungi according to the mass ratio of 7: 1, mixing the mixture in a reaction kettle, adding an ethanol solution which is 10 times of the mass of the substance obtained in the step (2) and has the mass fraction of 90%, stirring the mixture for 5 hours at the temperature of 30 ℃ and the rotating speed of 400r/min, filtering the mixture to obtain a filter cake, drying the filter cake at the temperature of 35 ℃ to obtain a blank, and mixing the blank and potassium humate according to the mass ratio of 12: 1, mixing;
(4) and (4) performing index analysis on the substance obtained in the step (3).
Optimally, the modifier in the step (2) is prepared by mixing potassium chloride and calcium chloride according to the mass ratio of 1: 2, mixing, adding hexadecyl trimethyl ammonium bromide which is 0.1 time of the mass of the potassium chloride, stirring and mixing to obtain the modifier.
Preferably, the mixed fungus in the step (3) is prepared by mixing bacillus pasteurii and acetobacter xylinum according to a mass ratio of 3: 1, mixing to obtain mixed fungi.
Comparative example:
the biological soil conditioner comprises the following raw material components in parts by weight: 30 parts of porous microspheres, 5 parts of potassium humate, 6 parts of mixed fungi and 5 parts of modifier.
A preparation process of a biological soil conditioner mainly comprises the following preparation steps:
(1) mixing bacterial cellulose and water according to the mass ratio of 1: 60, mixing the mixture in a beaker, adding potassium periodate which is 0.1-0.2 times of the mass of the bacterial cellulose into the beaker, stirring and reacting for 5 hours at the temperature of 50 ℃ and the rotation speed of 300r/min, performing suction filtration to obtain pretreated bacterial cellulose, mixing the pretreated bacterial cellulose and a glycol solution with the mass fraction of 10% according to the mass ratio of 1:40, stirring and reacting for 3 hours at the temperature of 40 ℃ and the rotation speed of 320r/min, performing suction filtration to obtain a modified bacterial cellulose blank, washing the modified bacterial cellulose blank with deionized water for 6 times, and performing freeze drying to obtain modified bacterial cellulose; mixing collagen and water according to a mass ratio of 1: 120, adding modified bacterial cellulose 2 times of collagen into a mixture of the collagen and water, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 320r/min, carrying out suction filtration to obtain a porous microsphere blank, washing the porous microsphere blank with deionized water for 3 times, and freeze-drying;
(2) mixing the substance obtained in the step (1) and a modifier according to a mass ratio of 8: 1, mixing, adding water which is 5 times of the mass of the substance obtained in the step (1), stirring and reacting for 3 hours at the temperature of 50 ℃ and the rotating speed of 320r/min, filtering, and freeze-drying;
(3) mixing the substances obtained in the step (2) with mixed fungi according to the mass ratio of 7: 1, mixing the mixture in a reaction kettle, adding an ethanol solution which is 10 times of the mass of the substance obtained in the step (2) and has the mass fraction of 90%, stirring the mixture for 5 hours at the temperature of 30 ℃ and the rotating speed of 400r/min, filtering the mixture to obtain a filter cake, drying the filter cake at the temperature of 35 ℃ to obtain a blank, and mixing the blank and potassium humate according to the mass ratio of 12: 1, mixing;
(4) and (4) performing index analysis on the substance obtained in the step (3).
Optimally, the modifier in the step (2) is prepared by mixing potassium chloride and calcium chloride according to the mass ratio of 1: 2, mixing, adding hexadecyl trimethyl ammonium bromide which is 0.1 time of the mass of the potassium chloride, stirring and mixing to obtain the modifier.
Preferably, the mixed fungus in the step (3) is prepared by mixing bacillus pasteurii and acetobacter xylinum according to a mass ratio of 3: 1, mixing to obtain mixed fungi.
Example of effects:
table 1 below shows the index analysis results of the biotype soil conditioner and the preparation process thereof using examples 1 to 3 of the present invention and the comparative example.
TABLE 1
It can be seen from the experimental data in table 1 that the addition of sodium bentonite and modified sodium lignosulfonate to the bio-type soil conditioner can effectively improve the acidity control and metal ion adsorption performance of the bio-type soil conditioner, thereby facilitating the growth of crops, as can be seen from comparison between examples 1 and 2, when no sodium bentonite is added to the product, no metal ion is attached to the modifier, so that during the use of the product, the product cannot fix hydrogen ions in the soil, and cannot form precipitates in cooperation with carbonate ions generated by bacillus pasteurii, thereby lowering the pH of the soil, and further lowering the acidity control of the product, as can be seen from comparison between examples 1 and 3, when no modified sodium lignosulfonate is added to the product, mixed colonies exist in the porous microspheres in the product, and during the storage process, the bacteria can decompose collagen in the porous microspheres, thereby reducing the usability of the product and greatly reducing the metal ion adsorption of the product due to the disappearance of active groups in the product.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference thereto is therefore intended to be embraced therein.
Claims (1)
1. A preparation process of a biological soil conditioner for reducing soil acidity is characterized by comprising the following specific preparation steps: (1) mixing bacterial cellulose and water according to the mass ratio of 1: 60, mixing the mixture in a beaker, adding potassium periodate which is 0.1-0.2 times of the mass of the bacterial cellulose into the beaker, stirring and reacting for 5 hours at the temperature of 50 ℃ and the rotation speed of 300r/min, performing suction filtration to obtain pretreated bacterial cellulose, mixing the pretreated bacterial cellulose and a glycol solution with the mass fraction of 10% according to the mass ratio of 1:40, stirring and reacting for 3 hours at the temperature of 40 ℃ and the rotation speed of 320r/min, performing suction filtration to obtain a modified bacterial cellulose blank, washing the modified bacterial cellulose blank with deionized water for 6 times, and performing freeze drying to obtain modified bacterial cellulose; mixing collagen and water according to a mass ratio of 1: 120, adding modified bacterial cellulose with the mass 2 times that of the collagen and sodium bentonite with the mass 0.5 times that of the collagen into a mixture of the collagen and water, stirring and reacting for 3 hours at the temperature of 45 ℃ and the rotating speed of 320r/min, carrying out suction filtration to obtain a porous microsphere blank, washing the porous microsphere blank with deionized water for 3 times, and freeze-drying; (2) mixing the substance obtained in the step (1) and a modifier according to a mass ratio of 8: 1, mixing, adding water which is 5 times of the mass of the substance obtained in the step (1), stirring and reacting for 3 hours at the temperature of 50 ℃ and the rotating speed of 320r/min, filtering, and freeze-drying; the modifier is prepared by mixing potassium chloride and calcium chloride according to a mass ratio of 1: 2, mixing, adding hexadecyl trimethyl ammonium bromide with the mass of 0.1 time of that of the potassium chloride, and stirring and mixing to obtain a modifier; (3) mixing modified sodium lignosulfonate with mixed fungi in a mass ratio of 3: 1, mixing the materials in a culture dish, adding a culture solution with the mass 8-10 times that of the modified sodium lignosulfonate into the culture dish, performing mixed culture at the temperature of 30 ℃ for 7 days, filtering, and drying at the temperature of 35 ℃; the modified sodium lignosulfonate is prepared by mixing sodium lignosulfonate and water in a mass ratio of 1: 12, adding 1, 6-dibromohexane accounting for 1.2 times of the mass of the sodium lignosulfonate and potassium iodide accounting for 0.10 time of the mass of the sodium lignosulfonate into a mixture of the sodium lignosulfonate and water, stirring and reacting for 5 hours at the temperature of 40 ℃ and the rotating speed of 300r/min, extracting with petroleum ether, filtering, removing an oil phase to obtain a modified sodium lignosulfonate blank, and freeze-drying the modified sodium lignosulfonate blank to obtain modified sodium lignosulfonate; the culture solution is prepared by mixing sucrose and beef extract according to a mass ratio of 4: 1, adding citric acid with the mass of 0.02 time that of sucrose, sodium hydrogen phosphate with the mass of 0.04 time that of sucrose, agar with the mass of 0.3 time that of sucrose, ethanol with the mass of 0.2 time that of sucrose and water with the mass of 20 times that of sucrose into a mixture of sucrose and beef extract, and stirring and mixing to obtain a culture solution; the mixed fungus is prepared by mixing bacillus pasteurii and acetobacter xylinum according to the mass ratio of 3: 1, mixing to obtain mixed fungi; (4) mixing the substance obtained in the step (2) with the substance obtained in the step (3) according to a mass ratio of 3: 1, mixing the mixture in a reaction kettle, adding an ethanol solution which is 10 times of the mass of the substance obtained in the step (2) and has the mass fraction of 90%, stirring and reacting for 5 hours at the temperature of 30 ℃ and the rotating speed of 400r/min, filtering to obtain a filter cake, drying the filter cake at the temperature of 35 ℃ to obtain a blank, and mixing the blank and potassium humate according to the mass ratio of 12: 1, mixing; obtaining the biological soil conditioner for reducing soil acidity.
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| CN114456008A (en) * | 2022-02-25 | 2022-05-10 | 峰景园林工程集团有限公司 | Modifying agent for improving tree transplanting survival rate and preparation method thereof |
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| CN111253951A (en) | 2020-06-09 |
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