CN111234830A - Microbial modifier for improving soil aggregate structure and application thereof - Google Patents
Microbial modifier for improving soil aggregate structure and application thereof Download PDFInfo
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Abstract
The invention discloses a microbial improver for improving a soil aggregate structure. The application of a microbial improver for improving a soil aggregate structure in soil improvement treatment. The functional microbial agent is bacillus amyloliquefaciens and bacillus belgii, has the effect of promoting the growth of crops, can prevent diseases and promote growth while improving soil; the soil improvement treatment soil conditioner is prepared by using activated humic acid, decomposed tobacco stem and tobacco powder, tobacco stem and tobacco powder active carbon, clay minerals and functional microbial agents as raw materials, so that the pH value of soil is always kept stable, the permeability of the soil can be improved, the water retention and storage capacity of the soil is enhanced, the cation exchange capacity of the soil is increased, the aims of improving the soil structure, improving the physicochemical property, improving the soil fertility, maintaining water and soil and improving the polluted soil are finally achieved, and good environmental conditions are provided for the growth and development of crops and microorganisms and the high and stable yield of the crops.
Description
Technical Field
The invention belongs to the technical field of microbial preparation, and particularly relates to a microbial improver for improving a soil granular structure and application thereof.
Background
The granular structure is named as the best soil structure because it has important coordination effect on the balance of water, fertilizer, gas, heat, bacteria and other aspects of soil. However, in recent years, problems of soil hardening, salinization and reduction of soil nutrient utilization rate caused by soil acidification aggravation are endless, and problems of unreasonable fertilization phenomenon, soil over-utilization and the like cannot be effectively inhibited, so that the soil granular structure is seriously damaged, the permeability and soil water suction of soil are reduced, the water retention capacity and soil cation exchange capacity of soil are reduced, and the growth of crops is not facilitated.
Disclosure of Invention
The first object of the present invention is to provide a microbial improver for improving a soil granular structure; the second purpose of the invention is to provide the application of the microbial improver for improving the soil granular structure in the soil improvement treatment.
The first object of the present invention is achieved by: a microbial improver for improving a soil aggregate structure comprises the following raw materials in parts by weight: 400-700 parts of decomposed tobacco stem and tobacco powder, 80-120 parts of tobacco stem and tobacco powder active carbon, 60-100 parts of activated humic acid, 5-10 parts of functional microbial powder, 50-120 parts of kaolin, 50-130 parts of montmorillonite powder and 50-120 parts of light calcium carbonate; the functional microbial powder comprises Bacillus subtilis WSWFJ-24 powder, and the preservation number is as follows: CGMCC No.18272, and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WSWFJ-25 powder, wherein the preservation number is as follows: CGMCC No.18262, the weight part ratio of Bacillus subtilis WSWFJ-24 to Bacillus amyloliquefaciens WSWFJ-25 is 3-5: 3-5, and the effective viable count of the Bacillus subtilis WSWFJ-24 and Bacillus amyloliquefaciens WSWFJ-25 powder is not less than 1000 hundred million units/g.
The second object of the present invention is achieved by: application of a microbial strain for improving a soil granular structure in soil improvement treatment.
Has the advantages that: the functional microbial agent is Bacillus belgii and Bacillus amyloliquefaciens, has the effect of promoting the growth of crops, can enhance the resistance of the crops to various microbial infection diseases such as bacterial wilt, blight and blackness to a certain extent, and can prevent diseases and promote growth while improving soil; the soil improvement treatment soil conditioner is prepared by using activated humic acid, decomposed tobacco stem and tobacco powder, tobacco stem and tobacco powder active carbon, clay minerals and functional microbial agents as raw materials, wherein functional groups in the activated humic acid interact with soil sticky particles to promote the formation of a soil water-stable granular structure, improve the relationship of pore spaces in soil, improve the total porosity of the soil, increase the air permeability and the air permeability, enhance the buffering performance of the soil, greatly improve the permeability and the soil water suction of the soil, enhance the water retention and storage capacity of the soil and increase the cation exchange capacity of the soil, thereby coordinating the solid-liquid-gas three-phase proportion in the soil, enabling the soil to have proper firmness, pH value and proper soil temperature and water content conditions, further enhancing the activity of soil microorganisms, improving the biological activity of the soil, increasing the release of quick-acting nutrients and finally achieving the purpose of improving the soil structure, The method aims at improving physicochemical property, improving soil fertility, maintaining water and soil and improving polluted soil, and provides good environmental conditions for crops and microorganism growth and development as well as high and stable yield of crops.
Detailed Description
The present invention is further illustrated but not limited in any way by the following description, and any alterations or substitutions based on the teachings of the present invention are intended to fall within the scope of the present invention.
The invention provides a microbial improver for improving a soil aggregate structure, which comprises the following raw materials in parts by weight: 400-700 parts of decomposed tobacco stem and tobacco powder, 80-120 parts of tobacco stem and tobacco powder active carbon, 60-100 parts of activated humic acid, 5-10 parts of functional microbial powder, 50-120 parts of kaolin, 50-130 parts of montmorillonite powder and 50-120 parts of light calcium carbonate.
The functional microbial powder comprises bacillus WSWFJ-24 powder and bacillus WSWFJ-25 powder, and the weight portion ratio of the two is 3-5: 3-5.
The effective viable count of the powder of the bacillus WSWFJ-24 and the bacillus WSWFJ-25 is not less than 1000 hundred million units/g
The preparation method of the tobacco stem and tobacco powder active carbon comprises the following steps: grinding tobacco stems and tobacco powder into tobacco powder, sieving the tobacco powder with a 60-mesh sieve, drying the tobacco powder, reacting the tobacco powder with 60% phosphoric acid activating agent for 24 hours to obtain a mixed sample, putting the mixed sample into a muffle furnace at 250 ℃ for carbonization for 4 hours, taking out the carbonized sample, putting the carbonized sample into a constant-temperature drying oven, and cooling the carbonized sample to room temperature to obtain the tobacco stem and tobacco powder active carbon.
The iodine adsorption value of the tobacco stem and tobacco powder active carbon is not less than 1100mg/g, and the BET specific surface area is not less than 1100m2The absorption aperture is 1-2 nm.
The Bacillus WSWFJ-24 is named as Bacillus belezii (Bacillus velezensis) WSWFJ-24, and the preservation unit is as follows: the China general microbiological culture Collection center has the following preservation addresses: xilu No.1 Hospital No. 3, Beijing, Chaoyang, on the day of deposit: 29/08/2019, with a deposit number: CGMCC No. 18272.
The bacillus WSWFJ-25 is named as bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WSWFJ-25, and the preservation unit is as follows: the China general microbiological culture Collection center has the following preservation addresses: xilu No.1 Hospital No. 3, Beijing, Chaoyang, on the day of deposit: 29/08/2019, with a deposit number: CGMCC No. 18262.
The bacillus WSWFJ-24 and the bacillus WSWFJ-25 are respectively prepared into fermentation liquor obtained by liquid culture fermentation culture, thallus obtained by solid fermentation culture medium fermentation culture, or mixture thallus of the solid culture medium and the thallus, and are further prepared into wet thallus, powder or freeze-dried powder.
The preparation method of the fermentation liquor of the bacillus WSWFJ-24 and the bacillus WSWFJ-25 comprises the following steps: preparing shake flask fermentation liquor by shake flask fermentation of the preserved bacillus WSWFJ-24 and the preserved bacillus WSWFJ-25, mixing 70 percent of the shake flask fermentation liquor of the bacillus WSWFJ-24 and the shake flask fermentation liquor of the bacillus WSWFJ-25 with 10 percent of filler white carbon black to prepare bacillus WSWFJ-24 mother liquor and bacillus WSWFJ-25 mother liquor, preparing bacillus WSWFJ-24 mother powder and bacillus WSWFJ-25 mother powder by air blast drying, and then adding 10 percent of PVA and 2 percent of K into the bacillus WSWFJ-24 mother powder and the bacillus WSWFJ-25 mother powder respectively3PO4And finally, adding β -cyclodextrin with the concentration of 0.1 percent, and complementing the balance with white carbon black to 100 percent, and finally, crushing by a jet mill to prepare the wettable powder of bacillus WSWFJ-24 mother powder and bacillus WSWFJ-25 mother powder.
The activated humic acid is black powder, and the pH value is 8-9.
The kaolin, the montmorillonite powder and the light calcium carbonate are materials which are sieved by a 60-mesh sieve.
The microbial modifier contains 45.5-55.3% of organic matter, 18.6-19.8% of water, 7.8-8.7 of pH and 4.2-8.6 hundred million units/g of effective viable count of functional microorganisms.
The application of a microbial improver for improving a soil aggregate structure in soil improvement treatment.
The present invention will be further described with reference to the following examples.
Example 1
A microbial improver for improving a soil aggregate structure comprises the following raw materials in parts by weight: 400 parts of decomposed tobacco stem and tobacco powder sieved by a 60-mesh sieve, 80 parts of activated carbon of the tobacco stem and tobacco powder, 60 parts of activated humic acid with the pH value of 8-9, 5 parts of functional microbial powder with the effective viable count not lower than 1000 hundred million units/g, 50 parts of kaolin sieved by the 60-mesh sieve, 50 parts of montmorillonite powder sieved by the 60-mesh sieve and 50 parts of light calcium carbonate sieved by the 60-mesh sieve; wherein the iodine adsorption value of the tobacco stem and tobacco powder active carbon is not less than 1100mg/g, and the BET specific surface area is not less than 1100m2The absorption aperture is 1-2 nm; the functional microbial powder comprises Bacillus belgii WSWFJ-24 and Bacillus amyloliquefaciens WSWFJ-25 powder, and the weight part ratio of the two is 3-5: 3-5.
The product contains 45.5 percent of organic matter, 18.6 percent of water, 7.8 of pH and 4.2 hundred million units/g of effective viable count of functional microorganism.
Example 2
A microbial improver for improving a soil aggregate structure comprises the following raw materials in parts by weight: 550 parts of decomposed tobacco stem and tobacco powder sieved by a 60-mesh sieve, 100 parts of activated carbon of the tobacco stem and tobacco powder, 80 parts of activated humic acid with the pH value of 8-9, 7 parts of functional microbial powder with the effective viable count not lower than 1000 hundred million units/g, 85 parts of kaolin sieved by the 60-mesh sieve, 90 parts of montmorillonite powder sieved by the 60-mesh sieve and 85 parts of light calcium carbonate sieved by the 60-mesh sieve; wherein the iodine adsorption value of the tobacco stem and tobacco powder active carbon is not less than 1100mg/g, and the BET specific surface area is not less than 1100m2The absorption aperture is 1-2 nm; the functional microbial powder comprises Bacillus belgii WSWFJ-24 and Bacillus amyloliquefaciens WSWFJ-25 powder, and the weight part ratio of the two is 3-5: 3-5.
The product contains 50% of organic matter, 19.2% of water, 8.2 of pH and 6.4 hundred million units/g of effective viable count of functional microorganisms.
Example 3
A microbial improver for improving a soil aggregate structure comprises the following raw materials in parts by weight: 700 parts of decomposed tobacco stem and tobacco powder and 120 parts of tobacco stem and tobacco powder active carbon which are sieved by a 60-mesh sieve, 100 parts of activated humic acid with the pH value of 8-9, and no effective viable count10 parts of functional microbial powder less than 1000 hundred million units/g, 120 parts of kaolin sieved by a 60-mesh sieve, 130 parts of montmorillonite powder sieved by the 60-mesh sieve and 120 parts of light calcium carbonate sieved by the 60-mesh sieve; wherein the iodine adsorption value of the tobacco stem and tobacco powder active carbon is not less than 1100mg/g, and the BET specific surface area is not less than 1100m2The absorption aperture is 1-2 nm; the functional microbial powder comprises Bacillus belgii WSWFJ-24 and Bacillus amyloliquefaciens WSWFJ-25 powder, and the weight portion ratio of the two is 3-5: 3-5.
The product contains 55.3 percent of organic matter, 19.8 percent of water, 8.7 of pH and 8.6 hundred million units/g of effective viable count of functional microorganisms.
Example 4
A microbial improver for improving a soil aggregate structure comprises the following raw materials in parts by weight: 400-700 parts of decomposed tobacco stem and tobacco powder sieved by a 60-mesh sieve, 80-120 parts of tobacco stem and tobacco powder active carbon, 60-100 parts of activated humic acid with the pH value of 8-9, 5-10 parts of functional microbial powder with the effective viable count not lower than 1000 hundred million units/g, 50-120 parts of kaolin sieved by the 60-mesh sieve, 50-130 parts of montmorillonite powder sieved by the 60-mesh sieve and 50-120 parts of light calcium carbonate sieved by the 60-mesh sieve; wherein the iodine adsorption value of the tobacco stem and tobacco powder active carbon is not less than 1100mg/g, and the BET specific surface area is not less than 1100m2The absorption aperture is 1-2 nm; the functional microbial powder comprises Bacillus belgii WSWFJ-24 and Bacillus amyloliquefaciens WSWFJ-25 powder, and the weight portion ratio of the two is 3-5: 3-5.
The microbial modifier contains 45.5-55.3% of organic matter, 18.6-19.8% of water, 7.8-8.7 of pH and 4.2-8.6 hundred million units/g of effective viable count of functional microorganisms.
Example 5
Influence of microbial improver for improving soil aggregate structure on soil physicochemical property in soil improvement treatment application
1. Purpose of experiment
The influence of the product provided by the invention on the physicochemical property of the soil is tested by taking the soil conservation as a starting point, so that a certain technical support is provided for the application of the product provided by the invention.
2. Materials and methods
2.1 the test site is Qinglong town of Anning city, Yunnan province microorganism fermentation engineering research center, Inc.
2.2 the test material is the microbial improver for improving the soil aggregate structure provided in embodiments 1 to 4 of the present invention.
2.3 design of the experiment
The test is carried out by adopting a cell mode, an open ground is divided into four cells, the area of each cell is 5m multiplied by 5m, 5Kg of the product provided by the invention is applied to each cell, the soil used for the test is loam, the initial pH of a soil layer of 0-20cm is 5.5 on average through measurement, the organic matter is 8.31g/Kg, the total nitrogen is 115.44mg/Kg, the quick-acting phosphorus is 12.87mg/Kg, the quick-acting potassium is 104.28mg/Kg, and the soil volume weight is 1.54g/cm3The test setup is detailed in table 1:
TABLE 1 design of the experiments
2.4 test management
Unified management is carried out during the test period, and samples are taken to determine the physical and chemical properties of the soil after ten days of treatment.
3. The results and analysis are shown in Table 2:
TABLE 2 influence of different formulations of the products provided by the invention on the physicochemical properties of the soil
From table 2, it can be seen that the products of the present invention treated with T1, T2, T3 and T4 all have an improvement effect on soil, the pH of soil is averagely increased by 0.8 compared with that before treatment, the organic matter is averagely increased by 1.02% compared with that before treatment, the total nitrogen is averagely increased by 0.51%, the available phosphorus is averagely increased by 0.95%, the available potassium is averagely increased by 1.20%, and the volume weight of soil is averagely decreased by 6.33%.
4. Discussion and conclusions
The product provided by the invention can obviously improve the physical and chemical properties of soil, increase the pH value of the soil, improve the content of organic matters in the soil and the content of nutrients in the soil, obviously reduce the volume weight of the soil of 0-20cm and thicken a soil plough layer.
Example 6
Influence of microbial improver for improving soil aggregate structure on growth of flue-cured tobacco in soil improvement treatment application
1. Purpose of the experiment
The tobacco is taken as a research object, the influence of the product provided by the invention on the growth of the flue-cured tobacco is discussed, and data and technical support are provided for the application and popularization of the product.
2. Test materials and methods
2.1 the test site was conducted in Mike Lauramura, Lu and Cui, Kunming, City.
2.2 test materials
The test varieties are: yunyan 87
Fertilizer to be tested: using local conventional fertilizer applications, the test set-up controls: conventional fertilization, demonstration treatment: the product provided by the invention is additionally applied on the basis of conventional fertilization.
2.3 design of the experiment
The demonstration adopts small-area field-by-field comparison, the local conventional fertilization formula is 10-12-24, 50kg of fertilizer is applied per mu, and a middle-layer ring-fertilization method is adopted as a base fertilizer for application. And the other water and fertilizer management is consistent with the local management.
2.4 survey items
And (5) surveying the agronomic characters of the flue-cured tobacco after 30 days, 50 days and 80 days of transplantation, and analyzing the results.
3. Analysis of results
3.1 the effect of the product provided by the invention on the growth of flue-cured tobacco in the bulk stage is shown in Table 3:
TABLE 3 Effect of the products of the present invention on the growth of the cured tobacco at the bulk stage
| Treatment of | Plant height | Stem enclosure | Pitch of | Number of blades | Maximum leaf length | Maximum leaf width |
| Control | 29.14 | 4.75 | 2.07 | 11.52 | 41.53 | 18.33 |
| Demonstration of | 29.46 | 4.83 | 2.04 | 12.17 | 42.86 | 18.70 |
Note: the units of the test data are cm.
As can be seen from Table 3, the plant height, stem circumference, leaf number, maximum leaf length and maximum leaf width of the flue-cured tobacco planted in the demonstration mode are all higher than those of the control area in the bulk period, and are respectively increased by 1.10%, 1.68%, 5.64%, 3.20% and 2.02% compared with the control area.
3.2 the influence of the product provided by the invention on the vigorous long-term growth of flue-cured tobacco is shown in Table 4:
table 4, the effect of the product provided by the invention on the vigorous long-term growth of flue-cured tobacco
Note: the units of the test data are cm.
As can be seen from Table 4, the plant height, stem circumference, pitch, leaf number, maximum upper leaf length and leaf width, maximum middle leaf length and leaf width, and maximum lower leaf length and leaf width of the flue-cured tobacco in the demonstration area where the product provided by the invention is applied are all higher than those in the control area in the vigorous growth period, and are respectively increased by 1.25%, 0.73%, 1.20%, 1.26%, 2.77%, 0.74%, 1.20%, 5.45%, 1.19% and 3.06% compared with the control area.
3.3 the effect of the product provided by the invention on the mature period growth of flue-cured tobacco is shown in Table 5:
TABLE 5 Effect of the products of the present invention on the mature stage growth of flue-cured tobacco
Note: the units of the test data are cm.
As can be seen from Table 5, the flue-cured tobacco added with the product provided by the invention has increased plant height, stem circumference, leaf number, maximum leaf length and leaf width of upper leaves, maximum leaf length and leaf width of middle leaves and maximum leaf length and leaf width of lower leaves compared with the control area in the vigorous growth period by 1.11%, 0.43%, 6.10%, 0.88%, 1.23%, 0.74%, 3.75%, 0.43%, 0.19% respectively.
4. Conclusion
From the analysis, the multiple agronomic indexes of the flue-cured tobacco in the group period, the vigorous growing period and the mature period are higher than those of the control in the product area provided by the invention, which shows that the application of the product provided by the invention plays a certain role in promoting the growth of the flue-cured tobacco and promotes the absorption of the tobacco plants to soil nutrients.
Claims (10)
1. A microbial amendment for improving soil aggregate structure, comprising: the feed comprises the following raw materials in parts by weight: 400-700 parts of decomposed tobacco stem and tobacco powder, 80-120 parts of tobacco stem and tobacco powder active carbon, 60-100 parts of activated humic acid, 5-10 parts of functional microbial powder, 50-120 parts of kaolin, 50-130 parts of montmorillonite powder and 50-120 parts of light calcium carbonate.
2. A microbial amendment for improving a soil aggregate structure according to claim 1, characterized in that: the functional microbial powder comprises 3-5 parts by weight of bacillus WSWFJ-24 and 3-5 parts by weight of bacillus WSWFJ-25 powder, and the number of effective viable bacteria in the bacillus WSWFJ-24 and the bacillus WSWFJ-25 powder is not less than 1000 hundred million units/g.
3. A microbial amendment for improving a soil aggregate structure according to claim 1, characterized in that: the preparation method of the tobacco stem and tobacco powder active carbon comprises the following steps: grinding tobacco stems and tobacco powder into tobacco powder, sieving the tobacco powder with a 60-mesh sieve, drying the tobacco powder, reacting the tobacco powder with 60% phosphoric acid activating agent for 24 hours to obtain a mixed sample, putting the mixed sample into a muffle furnace at 250 ℃ for carbonization for 4 hours, taking out the carbonized sample, putting the carbonized sample into a constant-temperature drying oven, and cooling the carbonized sample to room temperature to obtain the tobacco stem and tobacco powder active carbon.
4. A microbial amendment for improving a soil aggregate structure according to claim 1 or 3, characterized in that: the iodine adsorption value of the tobacco stem and tobacco powder active carbon is not less than 1100mg/g, and the BET specific surface area is not less than 1100m2The absorption aperture is 1-2 nm.
5. A microbial strain according to claim 2 for use in improving the structure of a soil granule, wherein: the Bacillus WSWFJ-24 is named as Bacillus belezii (Bacillus velezensis) WSWFJ-24, and the preservation number is as follows: CGMCC No. 18272.
6. A microbial strain according to claim 2 for use in improving the structure of a soil granule, wherein: the Bacillus WSWFJ-25 is named as Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) WSWFJ-25, and the preservation number is as follows: CGMCC No. 18262.
7. The microbial strain for improving soil granular structure according to claim 2, wherein: the bacillus WSWFJ-24 and the bacillus WSWFJ-25 are respectively prepared into fermentation liquor obtained by liquid culture fermentation culture, thallus obtained by solid fermentation culture medium fermentation culture, or mixture thallus of the solid culture medium and the thallus, and are further prepared into wet thallus, powder or freeze-dried powder; the preparation method of the fermentation liquor of the bacillus WSWFJ-24 and the bacillus WSWFJ-25 comprises the following steps: preparing shake flask fermentation liquor by shake flask fermentation of the preserved bacillus WSWFJ-24 and the preserved bacillus WSWFJ-25, mixing 70 percent of the shake flask fermentation liquor of the bacillus WSWFJ-24 and the shake flask fermentation liquor of the bacillus WSWFJ-25 with 10 percent of filler white carbon black to prepare bacillus WSWFJ-24 mother liquor and bacillus WSWFJ-25 mother liquor, preparing bacillus WSWFJ-24 mother powder and bacillus WSWFJ-25 mother powder by air blast drying, and then adding 10 percent of PVA and 2 percent of K into the bacillus WSWFJ-24 mother powder and the bacillus WSWFJ-25 mother powder respectively3PO4And finally, adding β -cyclodextrin with the concentration of 0.1 percent, and complementing the balance with white carbon black to 100 percent, and finally, crushing by a jet mill to prepare the wettable powder of bacillus WSWFJ-24 mother powder and bacillus WSWFJ-25 mother powder.
8. The microbial strain for improving soil granular structure according to claim 1, wherein: the activated humic acid is black powder, and the pH value is 8-9.
9. The microbial strain for improving soil granular structure according to claim 1, wherein: the microbial modifier contains 45.5-55.3% of organic matter, 18.6-19.8% of water, 7.8-8.7 of pH and 4.2-8.6 hundred million units/g of effective viable count of functional microorganisms.
10. Use of a microbial amendment according to any one of claims 1 to 9 for improving soil aggregate structure in a soil improvement treatment.
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| CN112279739A (en) * | 2020-10-16 | 2021-01-29 | 云南和瑾科技有限公司 | Parent-type soil remediation agent and preparation method thereof |
| CN112586509A (en) * | 2020-11-13 | 2021-04-02 | 遵义医科大学 | Compound microorganism effervescent tablet for preventing tobacco bacterial wilt and promoting growth, and preparation method and application thereof |
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