CN111228321A - Biological processing method of traditional Chinese medicine decoction pieces - Google Patents
Biological processing method of traditional Chinese medicine decoction pieces Download PDFInfo
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- CN111228321A CN111228321A CN202010204611.3A CN202010204611A CN111228321A CN 111228321 A CN111228321 A CN 111228321A CN 202010204611 A CN202010204611 A CN 202010204611A CN 111228321 A CN111228321 A CN 111228321A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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Abstract
The invention discloses a biological processing method of traditional Chinese medicine decoction pieces, which comprises the following steps: step1) to meet the purity requirement of pharmacopeia; step2) culturing rhodococcus; step3) biological processing: slicing the medicinal materials, putting the sliced medicinal materials into a rhodococcus solution, putting agar powder into the rhodococcus solution, fully mixing, wherein the concentration of the agar powder is 12-15g/L, and embedding the medicinal materials into slices for 18-25 hours at an ambient temperature of 27-38 ℃; step4) drying. The biological processing method of the traditional Chinese medicine decoction pieces adopts a low-temperature biological processing technology, thereby effectively retaining the beneficial components contained in the traditional Chinese medicine. In addition, the toxicity of the traditional Chinese medicine is obviously removed through the efficient degradation of the rhodococcus to the AFB 1.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine processing, in particular to a biological processing method of traditional Chinese medicine decoction pieces.
Background
The traditional Chinese medicine decoction pieces are prepared from traditional Chinese medicines according to the traditional Chinese medicine theory and the traditional Chinese medicine preparation method, and can be directly used as traditional Chinese medicines in traditional Chinese medicine clinical practice. The general traditional Chinese medicine decoction pieces are prepared by steaming and frying at high temperature, and the toxicity contained in the traditional Chinese medicine decoction pieces can be reduced after the steaming and frying. However, the beneficial elements contained in the steamed and fried traditional Chinese medicine decoction pieces are reduced due to evaporation and decomposition under the action of high temperature, so that the drug effect is reduced.
In addition, in large pharmaceutical factories, due to large medicine yield and various medicine types, a large amount of Chinese herbal medicines need to be stocked in a medicine warehouse for continuous medicine production, so that the storage time of some Chinese herbal medicines is as long as several years, and for the aged medicinal materials, if the medicinal materials are directly processed by a normal Chinese herbal medicine decoction piece method without treatment, more toxins such as aspergillus flavus (AFB1) and the like are left in the processed medicinal materials. At present, no report on how old medicinal materials are processed into traditional Chinese medicine decoction pieces is reported.
Disclosure of Invention
In view of the above, the present invention provides a biological processing method of decoction pieces of traditional Chinese medicine, which can significantly retain the beneficial components and the quality of the drug effect of traditional Chinese medicine. Meanwhile, the biological processing method can effectively remove the toxicity of the traditional Chinese medicine.
In order to achieve the purpose, the invention adopts the technical scheme that:
the biological processing method of the traditional Chinese medicine decoction pieces comprises the following steps:
step1) cleaning: cleaning the medicinal materials with clear water, soaking in 0.5-1.5% sodium hydroxide solution for 60-80 s, adding hydrochloric acid solution into the sodium hydroxide solution to adjust pH to neutral, standing for 60-70 s, and cleaning with clear water to reach the purity requirement of pharmacopoeia;
step2) culturing rhodococcus: firstly, preparing an LB liquid culture medium, wherein each liter of deionized water is added with 10g of tryptone, 5g of yeast extract and 10g of NaCl10g, then the pH value is adjusted to 7.0 by sodium hydroxide, and the LB liquid culture medium is prepared after autoclaving at 120 ℃ for 25 min; putting the rhodococcus into an LB liquid culture medium to be cultured for 2-3 days to obtain a rhodococcus solution;
step3) biological processing: slicing the medicinal materials, putting the sliced medicinal materials into a rhodococcus solution, putting agar powder into the rhodococcus solution, fully mixing, wherein the concentration of the agar powder is 12-15g/L, and embedding the medicinal materials into slices for 18-25 hours at an ambient temperature of 27-38 ℃;
step4) drying: taking the medicinal material slices in the step3 out of the LB culture medium containing the rhodococcus, cleaning, putting the medicinal material slices into a dryer for drying, controlling the drying temperature to be 40-55 ℃, and performing a sterilization step after drying, thereby preparing the biological processed traditional Chinese medicine decoction pieces.
Further, the rhodococcus is rhodococcus erythropolis.
Further, in the step1, the medicinal materials are cleaned by clear water and then are put into a 1% sodium hydroxide solution for soaking for 70 seconds.
Further, the environment temperature of the LB medium embedding the medicinal material slices in the step3 is 35 ℃.
The invention has the beneficial effects that:
1. the biological processing method of the traditional Chinese medicine decoction pieces adopts a low-temperature biological processing technology, thereby effectively retaining the beneficial components contained in the traditional Chinese medicine. In addition, the toxicity of the traditional Chinese medicine is obviously removed through the efficient degradation of the rhodococcus to the AFB 1.
2. The traditional Chinese medicine decoction pieces prepared by the biological processing method of the traditional Chinese medicine decoction pieces do not need to be processed at a high temperature, so that energy is saved.
Detailed Description
The invention mainly aims to provide a biological processing method of traditional Chinese medicine decoction pieces. In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described in detail with reference to specific embodiments.
The following takes ginseng, tuber fleeceflower root and kudzu root as examples, and the biological processing method of the traditional Chinese medicine decoction pieces is respectively used for processing:
example 1
The biological processing method of the traditional Chinese medicine decoction pieces comprises the following steps:
step1) cleaning: cleaning a ginseng medicinal material with clear water, soaking the ginseng medicinal material in a sodium hydroxide solution with the concentration of 0.5% for 60 seconds, adding a hydrochloric acid solution into the sodium hydroxide solution to adjust the pH to be neutral, standing for 60 seconds, and then cleaning with clear water to reach the purity requirement of pharmacopoeia;
step2) culturing rhodococcus: firstly, preparing an LB liquid culture medium, wherein each liter of deionized water is added with 10g of tryptone, 5g of yeast extract and 10g of NaCl10g, then the pH value is adjusted to 7.0 by sodium hydroxide, and the LB liquid culture medium is prepared after autoclaving at 120 ℃ for 25 min; putting the rhodococcus erythropolis into an LB liquid culture medium to be cultured for 2-3 days to obtain a rhodococcus erythropolis solution;
step3) biological processing: slicing the medicinal material, putting the sliced medicinal material into a Rhodococcus erythropolis solution, putting agar powder into the Rhodococcus erythropolis solution, fully mixing, wherein the concentration of the agar powder is 12g/L, and embedding the medicinal material slices for 18 hours at an ambient temperature of 27 ℃;
step4) drying: and (3) taking the medicinal material slices in the step (3) out of the LB culture medium containing the rhodococcus, cleaning, putting the medicinal material slices into a dryer for drying, controlling the drying temperature to be 40 ℃, and performing a sterilization step after drying to obtain the ginseng traditional Chinese medicine decoction pieces processed by the organisms.
Example 2
The biological processing method of the traditional Chinese medicine decoction pieces comprises the following steps:
step1) cleaning: cleaning polygonum multiflorum by using clear water, putting the polygonum multiflorum into a 1.5 percent sodium hydroxide solution, soaking for 80 seconds, adding a hydrochloric acid solution into the sodium hydroxide solution to adjust the pH to be neutral, standing for 70 seconds, and then cleaning by using clear water to meet the purity requirement of pharmacopoeia;
step2) culturing rhodococcus: firstly, preparing an LB liquid culture medium, wherein each liter of deionized water is added with 10g of tryptone, 5g of yeast extract and 10g of NaCl10g, then the pH value is adjusted to 7.0 by sodium hydroxide, and the LB liquid culture medium is prepared after autoclaving at 120 ℃ for 25 min; putting the rhodococcus into an LB liquid culture medium to be cultured for 2-3 days to obtain a rhodococcus solution;
step3) biological processing: slicing polygonum multiflorum and then placing the sliced polygonum multiflorum into a rhodococcus solution, placing agar powder into the rhodococcus solution to be fully mixed, wherein the concentration of the agar powder is 15g/L, and embedding the medicinal material slices for 25 hours at the environmental temperature of 38 ℃;
step4) drying: taking the polygonum multiflorum slices in the step3 out of the LB culture medium containing the rhodococcus, cleaning, putting the medicinal material slices into a dryer for drying, controlling the drying temperature to be 40-55 ℃, and performing a sterilization step after drying, thereby preparing the biologically processed polygonum multiflorum traditional Chinese medicine decoction pieces.
Example 3
The biological processing method of the traditional Chinese medicine decoction pieces comprises the following steps:
step1) cleaning: cleaning a kudzu root medicinal material with clear water, soaking the kudzu root medicinal material in a 1% sodium hydroxide solution for 70 seconds, adding a hydrochloric acid solution into the sodium hydroxide solution to adjust the pH value to be neutral, standing for 65 seconds, and then cleaning with clear water to reach the purity requirement of pharmacopoeia;
step2) culturing rhodococcus: firstly, preparing an LB liquid culture medium, wherein each liter of deionized water is added with 10g of tryptone, 5g of yeast extract and 10g of NaCl10g, then the pH value is adjusted to 7.0 by sodium hydroxide, and the LB liquid culture medium is prepared after autoclaving at 120 ℃ for 25 min; putting the rhodococcus into an LB liquid culture medium to be cultured for 2-3 days to obtain a rhodococcus solution;
step3) biological processing: slicing radix Puerariae, placing into Rhodococcus solution, placing agar powder into Rhodococcus solution, mixing thoroughly, wherein the concentration of agar powder is 14g/L, and embedding the slices at 35 deg.C for 18-25 hr;
step4) drying: and (3) taking the kudzu root slices in the step (3) out of the LB culture medium containing the rhodococcus, cleaning, putting the kudzu root slices into a dryer for drying, controlling the drying temperature to be 50 ℃, and then performing a sterilization step after drying, thereby preparing the bio-processed kudzu root traditional Chinese medicine decoction pieces.
The contents of the beneficial elements in the decoction pieces of the Chinese traditional medicine prepared in the examples 1 to 3 are compared with the contents of the beneficial elements in the decoction pieces of the Chinese traditional medicine respectively prepared by adopting the conventional processing method, and the contents are shown in the following table:
| example 1 | Example 2 | Example 3 | |
| Ginsenoside in Ginseng radix | 11.5％ | 12.2％ | 10.7％ |
| Emodin in Polygoni Multiflori radix | 15.0％ | 17.1％ | 16.5％ |
| Puerarin in radix Puerariae | 15.6％ | 15.7％ | 14.8％ |
From the detected data, the beneficial components contained in the Chinese medicinal decoction pieces prepared by the biological processing method of the invention are obviously higher than those in the Chinese medicinal decoction pieces prepared by high-temperature processing.
The above description is intended to describe in detail the preferred embodiments of the present invention, and the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (4)
1. A biological processing method of traditional Chinese medicine decoction pieces is characterized by comprising the following steps:
step1) cleaning: cleaning the medicinal materials with clear water, soaking in 0.5-1.5% sodium hydroxide solution for 60-80 s, adding hydrochloric acid solution into the sodium hydroxide solution to adjust pH to neutral, standing for 60-70 s, and cleaning with clear water to reach the purity requirement of pharmacopoeia;
step2) culturing rhodococcus: firstly, preparing an LB liquid culture medium, wherein each liter of deionized water is added with 10g of tryptone, 5g of yeast extract and 10g of NaCl10g, then the pH value is adjusted to 7.0 by sodium hydroxide, and the LB liquid culture medium is prepared after autoclaving at 120 ℃ for 25 min; putting the rhodococcus into an LB liquid culture medium to be cultured for 2-3 days to obtain a rhodococcus solution;
step3) biological processing: slicing the medicinal materials, putting the sliced medicinal materials into a rhodococcus solution, putting agar powder into the rhodococcus solution, fully mixing, wherein the concentration of the agar powder is 12-15g/L, and embedding the medicinal materials into slices for 18-25 hours at an ambient temperature of 27-38 ℃;
step4) drying: and (3) taking the medicinal material slices in the step (3) out of the LB culture medium containing the rhodococcus, cleaning, putting the medicinal material slices into a dryer for drying, controlling the drying temperature to be 40-55 ℃, and performing a sterilization step after drying. Thus preparing the biological processed traditional Chinese medicine decoction pieces.
2. The bioprocessing method of herbal pieces of decoction of traditional Chinese medicine according to claim 1, wherein said rhodococcus is rhodococcus erythropolis.
3. The bioprocessing method of herbal pieces ready for decoction of chinese medicine of claim 1, wherein in step1, the herbal pieces are washed with clean water and then soaked in 1% sodium hydroxide solution for 70 seconds.
4. The bioprocessing method of herbal pieces ready for decoction of traditional Chinese medicine of claim 3, wherein the environmental temperature of the LB medium embedding the herbal pieces in step3 is 35 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010204611.3A CN111228321A (en) | 2020-03-22 | 2020-03-22 | Biological processing method of traditional Chinese medicine decoction pieces |
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| CN202010204611.3A CN111228321A (en) | 2020-03-22 | 2020-03-22 | Biological processing method of traditional Chinese medicine decoction pieces |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112516251A (en) * | 2020-12-29 | 2021-03-19 | 联和东浩(厦门)药业有限公司 | Anoectochilus roxburghii traditional Chinese medicine decoction pieces and production process thereof |
-
2020
- 2020-03-22 CN CN202010204611.3A patent/CN111228321A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 呙于明主编: "《动物营养研究进展2016》", 31 October 2016, 中国农业大学出版社 * |
| 赵东睿: "中药中黄曲霉毒素的检测方法及防治措施", 《健康之友》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112516251A (en) * | 2020-12-29 | 2021-03-19 | 联和东浩(厦门)药业有限公司 | Anoectochilus roxburghii traditional Chinese medicine decoction pieces and production process thereof |
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